EXPERIMENTAL CELL RESEARCH
·
2023
·
IF 3.7
Hanqing Zhu,Hongliang Zhang,Jiahui Guo,Chao Zhang,Quanwu Zhang,Fenghou Gao
Mdm2 promotes the ubiquitination and degradation of p53, while Mdm2-p60 can bind to p53 and reduce the Mdm2-induced p53 ubiquitination to improve its stability. USP2a can deubiquitinate and stabilize Mdm2, whether USP2a can regulate Mdm2-p60 needs to be further confirmed and elucidated. We found that oxidative stress can up-regulate USP2a at the post-transcriptional level and induce USP2a to be oxidized by forming inter-subunit disulfide bonds . The oxidized USP2a is closely related with cell apoptosis. In apoptotic cells, oxidized USP2a has enhanced protein stability and further stabilizes Mdm2-p60 through deubiquitination , and the USP2a-Mdm2-p60-p53 axis plays a role in cell apoptosis. Altogether USP2a is oxygen sensitive, oxidized USP2a exerts apoptotic effects through the Mdm2-p60-p53 axis, which provides an experimental basis for regulating p53 apoptotic signaling by targeting USP2a.
研究领域:
细胞生物学
·
分子生物学
·
癌症研究
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产品关联
DOI
PubMed
高影响因子
Polymer Chemistry
·
2021
·Vol. 12
·期 26
·3832-3841
·
IF 5.582
Taisheng Wang,Haoxiang Wang,Lei Shen,Na Zhang
Mechanochromic polymers (MCPs), which respond to mechanical stimuli via an optical signal change, are regarded for their application in smart materials, such as force sensors. Although rapid progress in MCPs has been witnessed in recent years, MCPs systems that can distinguish different magnitudes of stretching or compression stress through multicolor transformation are still hard to achieve. In this paper, we report our findings on a multicolor mechanochromic elastomer that can discriminate between low and high stretching stress. The key point of our design depends on the introduction of two mechanophores, which present different fluorescence changes in response to mechanical stimuli. These mechanophores were elaborately introduced to the multi-network elastomer though a multiple swelling–UV polymerization process. Under relatively low tensile stress (≲1.8 MPa), the formation of a blue-fluorescence anthracene derivative was observed, while under high tensile stress (≳1.8 MPa), both mechanophores were activated. Thus, the elastomer exhibited a blue-white and further red fluorescence. Although the present results remain preliminary, the macroscopic fluorescence change at different levels of stress could indicate where the stress is concentrated, which would be beneficial to elucidate the mechanism of material failures.
研究领域:
材料科学
·
化学
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DOI
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2014
·
IF 2.009
Rong-Yu Li,Xiao-Mao Wu,Xian-Hui Yin,You-Hua Long,Ming Li
Given the importance of finding alternatives to synthetic fungicides, the antifungal effects of natural product citral on six plant pathogenic fungi ( Magnaporthe grisea, Gibberella zeae, Fusarium oxysporum, Valsa mali, Botrytis cinerea , and Rhizoctonia solani ) were determined. Mycelial growth rate results showed that citral possessed high antifungal activities on those test fungi with EC 50 values ranging from 39.52 to 193.00 µg/mL, which had the highest inhibition rates against M. grisea . Further action mechanism of citral on M. grisea was carried out. Citral treatment was found to alter the morphology of M. grisea hyphae by causing a loss of cytoplasm and distortion of mycelia. Moreover, citral was able to induce an increase in chitinase activity in M. grisea , indicating disruption of the cell wall. These results indicate that citral may act by disrupting cell wall integrity and membrane permeability, thus resulting in physiology changes and causing cytotoxicity. Importantly, the inhibitory effect of citral on M. grisea appears to be associated with its effects on mycelia reducing sugar, soluble protein, chitinase activity, pyruvate content, and malondialdehyde content.
研究领域:
植物病理学
·
真菌学
·
天然产物化学
·
农业科学
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DOI
PubMed
高影响因子
EXPERIMENTAL BIOLOGY AND MEDICINE
·
2020
·
IF 3.139
Xue-Liang Dang,Long-Fei Yang,Lei Shi,Long-Fei Li,Ping He,Jie Chen,Bei-Jie Zheng,Peng Yang,Ai-Dong Wen
Overdose of acetaminophen (APAP) is responsible for the most cases of acute liver failure worldwide. Hepatic mitochondrial damage mediated by neuronal nitric oxide synthase- (nNOS) induced liver protein tyrosine nitration plays a critical role in the pathophysiology of APAP hepatotoxicity. It has been reported that pre-treatment or co-treatment with glycyrrhizin can protect against hepatotoxicity through prevention of hepatocellular apoptosis. However, the majority of APAP-induced acute liver failure cases are people intentionally taking the drug to commit suicide. Any preventive treatment is of little value in practice. In addition, the hepatocellular damage induced by APAP is considered to be oncotic necrosis rather than apoptosis. In the present study, our aim is to investigate if glycyrrhizin can be used therapeutically and the underlying mechanisms of APAP hepatotoxicity protection. Hepatic damage was induced by 300 mg/kg APAP in balb/c mice, followed with administration of 40, 80, or 160 mg/kg glycyrrhizin 90 min later. Mice were euthanized and harvested at 6 h post-APAP. Compared with model controls, glycyrrhizin post-treatment attenuated hepatic mitochondrial and hepatocellular damages, as indicated by decreased serum glutamate dehydrogenase, alanine aminotransferase, and aspartate aminotransferase activities as well as ameliorated mitochondrial swollen, distortion, and hepatocellular necrosis. Notably, 80 mg/kg glycyrrhizin inhibited hepatic nNOS activity and its mRNA and protein expression levels by 16.9, 14.9, and 28.3%, respectively. These results were consistent with the decreased liver nitric oxide content and liver protein tyrosine nitration indicated by 3-nitrotyrosine staining. Moreover, glycyrrhizin did not affect the APAP metabolic activation, and the survival rate of ALF mice was increased by glycyrrhizin. The present study indicates that post-treatment with glycyrrhizin can dose-dependently attenuate hepatic mitochondrial damage and inhibit the up-regulation of hepatic nNOS induced by APAP. Glycyrrhizin shows promise as drug for the treatment of APAP hepatotoxicity.
研究领域:
药理学
·
肝病学
·
毒理学
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DOI
PubMed
高影响因子
Communication Culture & Critique
·
2015
·Vol. 9
·期 4
·499-516
·
IF 0
Darling-Wolf Fabienne
In the United States, anime is often branded as a quintessentially Japanese genre whose attractiveness to foreign audiences comes from its mix of exoticism and universal human values. Some animated texts do not, however, easily fit this characterization. One example is the series Heidi, Girl of the Alps, which spread to some 35 countries starting in the late 1970s. This article explores the consequences of scholars' failure to engage with Heidi despite its significance as an extremely globally influential text and an exemplar of Miyazaki's early work. Exploring how Heidi resonates with dimensions of Japanese culture and of Miyazaki's oeuvre, it demonstrates how “reclaiming” Heidi can help us develop a more sophisticated understanding of transcultural dynamics under conditions of globalization.
✓ 已验证
DOI
NATURE IMMUNOLOGY
·
2024
·
IF 27.7
Xia Yun,Li Xin,Bie Nana,Pan Wen,Miao Ya-Ru,Yang Mei,Gao Yan,Chen Chuang,Liu Hanqing,Gan Lu,Guo An-Yuan
Combination therapy is a promising therapeutic strategy to enhance the efficacy of immune checkpoint blockade (ICB); however, predicting drugs for effective combination is challenging. Here we developed a general data-driven method called CM-Drug for screening compounds that can boost ICB treatment efficacy based on core and minor gene sets identified between responsive and nonresponsive samples in ICB therapy. The CM-Drug method was validated using melanoma and lung cancer mouse models, with combined therapeutic efficacy demonstrated in eight of nine predicted compounds. Among these compounds, taltirelin had the strongest synergistic effect. Mechanistic analysis and experimental verification demonstrated that taltirelin can stimulate CD8 + T cells and is mediated by the induction of thyroid-stimulating hormone. This study provides an effective and general method for predicting and evaluating drugs for combination therapy and identifies candidate compounds for future ICB combination therapy.
研究领域:
肿瘤学
·
免疫治疗
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
MATERIALS LETTERS
·
2019
·
IF 3.019
Cheng-Long Yu,Hang Liu,Jie Song,Jing Yang,Peng-Cheng Zhang,Fang-Lin Guo,Lin Hong,Huai-Bing Hui
We have reported for the first time the synthesis of mixed anatase and rutile TiO 2 under 27 MPa in water at the temperature as low as 375 °C. XRD patterns show that the content of rutile in the mixed phase TiO 2 is increased by 47.7% compared with the raw TiO 2 . The average particle size of the mixed phase TiO 2 is increased obviously compared with the raw TiO 2 . Compared with that of the raw TiO 2 , the band gap of the mixed phase TiO 2 is reduced to 2.94 eV while the absorbance is enhanced. Although the particle size increases, the mixed phase TiO 2 still exhibits better photocatalytic performance.
研究领域:
材料科学
·
无机化学
·
纳米材料
·
光催化
·
固体化学
✓ 已验证
DOI
高影响因子
EUROPEAN POLYMER JOURNAL
·
2020
·
IF 3.862
Xunzheng Su,Zhen Zhou,Jingcheng Liu,Jing Luo,Ren Liu
In this paper, a high-performance epoxy resin was synthesized from vanillin , and the polyimine-epoxy cross-linked network was prepared via in-situ formation of the Schiff base structure. The chemical structures, thermal and mechanical properties of vanillin-based epoxy resin (DADE) and diglycidyl ether of bisphenol A epoxy resin (DGEBA) were characterized by fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance spectra ( 1 H NMR), differential scanning calorimetry (DSC), and dynamic thermomechanical analysis (DMA). The results indicated that the DADE showed better thermal and mechanical properties than DGEBA after cured by the same primary amine (D230). The prepared DADE-D230 showed higher Tg (106℃), tensile strength (57.4 MPa) and elongation at break (3.1%) than DGEBA-D230 (98℃, 45.1 MPa, and 2.4%, respectively). Moreover, it can be recycled at a hot pressing, and the reprocessed samples showed a comparable performance with DGEBA.
研究领域:
材料科学
·
化学工程
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产品关联
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2022
·
IF 10.53
Lan-Qian Li,Yong-Jian Tang,Zhen-Liang Xu
In the practical application of nanofiltration (NF) membrane, regular chemical cleaning is necessary to eliminate the membrane fouling and ensure the normal operation of the system. Generally, the commercial NF membranes can be divided into MPD-TMC membranes and PIP-TMC membranes of which the permeability and pH resistance cannot be balanced. In this work, the ultra-low concentration monomers of MPD and TMC were used to fabricate the dense polyamide layer of NF membrane through a conventional interfacial polymerization reaction. The newly prepared membrane exhibited a smooth surface with very low roughness. Besides, the pore size distribution and desalination performance including water permeability and salt rejection of our membrane were comparable to the lab-made and commercial PIP-TMC membranes. The membrane was also confirmed to have an excellent separation efficiency to SO 4 2− /Cl − anions and good resistance to extreme pH conditions. Overall, this study presents a new insight for the preparation of high-performance NF membrane with good chemical stability.
研究领域:
材料科学
·
环境工程
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Chunyan Liao,Xiang Wang,Xueying Zhou,Dequan Wang,Ziyin Zhang,Yan Liu,Xiao Wu,Ying Chen,Yifeng Tan,Xin Dai,Pei Jing,Jie Pang,Xiao Xiao,Jie Liu,Xiaoming Liao,Shiyong Zhang
Despite great advances, the development of cancer drugs that can efficiently kill cancer cells while protecting noncancer cells has not been achieved. By using only dietary antioxidants vitamin C (VC) and (R)-(+)-lipoic acid (LA), we herein develop a nanodrug VC@cLAV featuring the above function. After entering cells, cLAV dissociates into LA and DHLA (dihydrolipoic acid, reduced form of LA) and releases VC and DHA (dehydroascorbate, oxidized form of VC). In cancer cells, the two redox pairs recycle each other and dramatically promote the intracellular reactive oxygen species production to kill cancer cells at low doses comparable to cytotoxic drugs. Oppositely in noncancer cells, the LA/DHLA and VC/DHA pairs exert anti-oxidant action to actively protect the organism by preventing the normal cells from oxidative stress and repairing cells suffering from oxidative stress. When compared with the first-line cytotoxic drug, VC@cLAV displayed superior therapeutic outcomes yet without side effects in diverse tumor models including patient-derived xenograft (PDX). This drug with efficient cancer cell killing and noncancer cell protection represents a new cancer therapy.
研究领域:
药学
·
肿瘤学
·
纳米技术
·
药物开发
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEUROMOLECULAR MEDICINE
·
2022
·Vol. 25
·期 1
·53-63
·
IF 4.103
Cheng Jing,Fan Yan-Qin,Zhang Wen-Fei,Zhang Guo,Zeng Kuo,Ye Zhang,Zhao Dan,Wu Li-Quan,Chen Zhi-Biao
Sirtuin-6 (SIRT6), a member of the sirtuins family of NAD ( +) dependent deacetylases, has been shown to have beneficial effects in ischemic stroke. However, the role of SIRT6 in intracerebral haemorrhage (ICH) has not reported. We observed that SIRT6 expression was down-regulated in human ICH patients and down-regulated in ICH-induced rat cortical neurons. We subsequently found that SIRT6 overexpression reduced brain tissue damage and increased neuronal survival in the ICH model of rats and hemin-induced cortical neurons. Our further study found that overexpression of SIRT6 can reduce inflammatory response by down-regulating the expression of NF-kB and thus promote the recovery of neurological function in ICH animals. In conclusion, SIRT6 can inhibit the expression of NF-kB and plays a neuroprotective role in ICH by inhibiting the NF-kB-mediated inflammatory response.SIRT6 could be a novel therapeutic target for ICH.
研究领域:
神经科学
·
分子生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Water
·
2025
·Vol. 17
·期 2
·161
·
IF 3
Nan Xiong,Yansen Guo,Yanyan Nie,Yuqing Yao,Zhemian Ying,Wei Zhang,Renduo Liu,Xiaoling Wu,He Zhou,Limin Zhou,Ying Wang,Jian He,Long Yan
As a well-known photocatalyst, TiO2still suffers from rapid electron–hole recombination and limited visible light absorption. To overcome these challenges, the combination of graphene and TiO2has been proposed. However, traditional methods such as ball milling and hydrothermal synthesis face limitations, including high energy consumption and complex procedures. Here, we develop a simple and industrially feasible method to prepare reduced graphene oxide (rGO)-coated TiO2nanoparticles, referred to as rGO-TiO2composites. The optimized rGO-TiO2composites exhibit an enhanced photocatalytic degradation of rhodamine B (RhB) under simulated sunlight conditions, about 99.95% for 4% rGO-TiO2within 80 min. The first-order reaction rate constant (k) of 4% rGO-TiO2(0.0867 min−1) is 5.42 times higher than that of nano TiO2(0.0135 min−1). The key reactive species involved in the degradation process are identified. Additionally, the effects of pH and NaCl concentration on the degradation efficiency of rGO-TiO2are also investigated. The 4% rGO-TiO2composite exhibits an excellent photocatalytic activity within the pH range of 3.87–11.89, and the NaCl concentration does not affect its photocatalytic efficiency. After characterization, the enhanced photocatalytic activity is ascribed to the introduction of rGO and the generation of surface oxygen vacancies (OV) and Ti3+in TiO2crystals.
研究领域:
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）