INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
·
IF 8.2
Zhe Li,Ao Liu,Hailian Wu,Abid Naeem,Qimeng Fan,Zhengji Jin,Hongning Liu,Liangshan Ming
Cellulose nanocrystals (CNCs) extracted from the waste shell of Camellia oleifera Abel ( C. oleifera ) are gaining attention as valuable materials. In this study, CNCs were extracted from the agricultural waste shell of C. oleifera through phosphoric acid and sulfuric acid hydrolysis , respectively. Firstly, we optimized the alkaline treatment process for cellulose isolation by using response surface methodology. Furthermore, the properties of CNCs were investigated by neutralizing them with NaOH and NH 3 ·H 2 O, and by dialysis in water. In addition, the characterization methods including FT-IR, TGA , AFM and TEM were used to analysis the properties of the synthesized CNCs. Finally, CNCs were studied for their application in essential oil-based Pickering emulsions. CNCs obtained from sulfuric acid showed the smallest particle size and good dispersibility. Moreover, the release profiles of essential oils in the emulsions were followed by Peppa's kinetic release model. The antibacterial activity of the emulsions against E. coli and S. aureus showed that CNCs-stabilized emulsions enhanced the antibacterial activity of essential oils. Therefore, neutralization treatments may enhance the properties of CNCs, and CNCs stabilized Pickering emulsions can enhance antibacterial activity of essential oil. This study provides insight into the potential application of CNCs derived from C. oleifera waste shells.
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Frontiers in Pharmacology
·
2024
·
IF 4.4
Niu Jingming,Jia Xiaoyan,Yang Nian,Ran Yuanquan,Wu Xia,Ding Furong,Tang Dongxin,Tian Minyi
Camellia oleifera is a medicine food homology plant widely cultivated in the Yangtze River Basin and southern China due to its camellia oil. Camellia oleifera bud and fruit exist simultaneously, and its bud is largely discarded as waste. However, C. oleifera bud has been used in traditional Chinese medicine to treat a variety of ailments. Thus, the purpose of this study was to identify the chemical components of C. oleifera bud ethanol extract (EE) and first evaluate its anticancer effects in non-small cell lung cancer A549 cells. Based on UHPLC-Q-Orbitrap-MS analysis, seventy components were identified. For anticancer activity, C. oleifera bud EE had remarkable cytotoxic effect on non-small cell lung cancer A549 (IC50: 57.53 ± 1.54 μg/mL) and NCI-H1299 (IC50: 131.67 ± 4.32 μg/mL) cells, while showed lower cytotoxicity on non-cancerous MRC-5 (IC50 > 320 μg/mL) and L929 (IC50: 179.84 ± 1.08 μg/mL) cells. It dramatically inhibited the proliferation of A549 cells by inducing cell cycle arrest at the G1 phase. Additionally, it induced apoptosis in A549 cells through a mitochondria-mediated pathway, which decreased mitochondrial membrane potential, upregulated Bax, activated caspase 9 and caspase 3, and resulted in PARP cleavage. Wound healing and transwell invasion assays demonstrated that C. oleifera bud EE inhibited the migration and invasion of A549 cells in a dose-dependent manner. The above findings indicated that C. oleifera bud EE revealed notable anticancer effects by inhibiting proliferation, inducing apoptosis, and suppressing migration and invasion of A549 cells. Hence, C. oleifera bud ethanol extract could serve as a new source of natural anticancer drugs.
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FEMS MICROBIOLOGY LETTERS
·
2018
·Vol. 365
·期 7
·
IF 1.735
Qian Bingjun,Yin Lirong,Yao Xiaomin,Zhong Yaoguang,Gui Juan,Lu Feifeng,Zhang Fuming,Zhang Jianhua
The saponins, as components of tea seed meal, are undesirable hemolytic components and should be degraded for reducing their hemolytic activity in order to be used in animal feed. In this study, β-glucuronidase was verified to be a potent hydrolase of tea seed saponins to reduce their hemolytic activity and a β-glucuronidase-producing Lactobacillus crustorum strain was screened from raw bovine milk. Next, solid-state fermentation with the isolated L. crustorum and a Bacillus subtilis natto strain, which can produce cellulase and hence improve the fermentation performance of tea seed meal, was carried out for detoxification of tea seed meal. The 50% hemolytic dosage (HD50) value of tea seed saponins was increased from 6.69 to 27.43 μg mL−1. The results of LC–MS analysis showed that the percentage of saponin aglycones increased from 30.95 to 84.25% after the fermentation. According to the roles of sugar moieties in hemolytic activity, and the enzymatic hydrolysis characteristics of β-glucuronidase, the degradation of tea seed saponins from glucosides to aglycones may contribute to the reduction of hemolytic activity. Therefore, tea seed meal may be used as animal feed after fermentation with the tested saponin-degrading microbial strains.
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INDUSTRIAL CROPS AND PRODUCTS
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2024
·
IF 5.6
Changyang Qiu,Zhihong Chen,Fenjuan Hu,Hailing Wu,Lu Wang,Meixia Liu,Kai Zhou
The seed cake of Camellia oleifera Abel. (SCOC), a big residue produced after Camellia oil extraction, enriches with many bioactive components. However, it is often discarded directly due to the lack of cost-effective extraction. Herein, a three-phase partitioning (TPP) system is first time tried for the simultaneous extraction of three major ingredients i.e. residual oil, protein, and polysaccharide from SCOC. Results from the extraction yields show that the established method is more efficient or at least comparable to conventional techniques. The developed method takes only 1.5 h and can be carried out at mild temperature of 40 °C, making it a cost-effective method. The successful scaling up of the extraction system at a 30 L extraction tank confirms its scalability. Furthermore, compared with conventional extraction methods, the TPP extracts shows better product quality. These facts, along with simultaneous extraction features of the method, allow us to use TPP as a promising extraction technology of SCOC biomolecules.
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POLYMER COMPOSITES
·
2024
·
IF 4.8
Xianyue Liang,Zhixiang Huang,Yonghui Zhou,Chuanshuang Hu,Dengyun Tu,Litao Guan
The preparation of wood-plastic composites (WPCs) with high filling fibers and excellent physical properties remained a great challenge. This study used Camellia oleifera shell (COS, agroforestry product waste) as raw material, and prepared 60 wt% COS/high-density polyethylene (HDPE) WPCs using profile extrusion method through pretreating fibers including hot water, hot steam, alkali, acid, extraction, and fine fiber screening. After pretreatments, the relative contents of cellulose and lignin increased, the relative contents of hemicellulose and extract decreased significantly, and the length-diameter ratio was improved. The flexural strength, tensile strength and impact strength of hot water and steam heat treated composites reached 31.4, 17.1 MPa and 6.9 kJ/m 2 , 31.8, 16.8 MPa and 6.7 kJ/m 2 , respectively. Compared with untreated 28.8, 14.7 MPa and 5.5 kJ/m 2 , the advantages were more obvious. Alkali treatment and acid treatment improved the thermal stability and residue rate of the material. Extraction treatment and alkali treatment significantly reduced the processing torque force, which was conducive to processing, while acid treatment was the opposite. The highest water absorption and thickness expansion rate were 13.75% and 3.60% respectively at 7 days after alkali treatment, which were considerably higher than 5.60% and 0.78% without treatment, while other pretreatments improved the dimensional stability of the material. Highlights High filling Camellia oleifera shell waste/HDPE composites were prepared. Physical and chemical pretreatments improved interfacial adhesion of the composites. Heat treatments significantly improved mechanical performance of the composites.
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Journal of Energy Storage
·
2025
·
IF 9.8
Lihua Cao,Bingchen Li,Jie Zeng,Xinyan Lin,Caizhi Zeng,Yafang Zhang,Jianbing Liu,Fei Xia,Li Zhang,Zhiyong Xu,Xue Hou
The discontinuous ion and electronic transport caused by randomly distributed powders lead to inferior ionic conductivity and large electronic transmission resistance. All carbon hybrid films electrode, the synergistic combination Camellia Oleifera pollen with reduced graphene oxide (rGO) greatly improves the operation of conductive carbons and doping, and can be easily adjusted from 3D doped porous carbon (3DPC), self-standing 3D doped carbon hybrid films composed of porous carbon and graphene (3DPC-G), to self-standing 3D graphene films (3DG), can be an excellent method. The high oxygen (28.6 %), nitrogen (3.29 %) and sulfur (0.91 %) content of 3DPC-G film electrode, in which porous carbon tightly adhered to the surface of graphene to form complementary synergistic effects of conductivity and 3D interconnected hierarchical porous structure, can efficiently promote the electron transfer and ion transport. Thus, 3DPC-G-based supercapacitor achieved an impressive energy density of 14.6 Wh kg −1 (71.3 mWh cm −2 ) and cycle stability (>90 % after 10,000 cycles). Meanwhile, 3DPC-G//Zn ZIHC delivered a high energy density of 190.89 mWh cm −2 at 0.22 mW cm −2 and long cycle stability (83.5 % after 10,000 cycles). Such a synergistically enhance films may throw light on the rational design of carbon electrodes for practical high-performance flexible supercapacitors.
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BIORESOURCE TECHNOLOGY
·
2020
·
IF 7.539
Yanzhi You,Xiankun Zhang,Pengfei Li,Fuhou Lei,Jianxin Jiang
Camellia oleifera shell (COS) is a worthy byproduct in woody edible oil production enriched in hemicellulose and lignin. This paper aims to explore the high-value transformation of COS for the production of xylooligosaccharides (XOS) with main degree of polymerization (DP) of 2–5 by the catalysis of ZnCl 2 . The effect of pretreatment temperature, reaction time and ZnCl 2 concentration on the contents and DP distributions of XOS were analyzed. Moderate reaction conditions tended to achieve high content XOS, and the maximum value 61.38% and 14.39 g/L of XOS yield and concentration, respectively, peaked at 170 °C for 30 min using 0.5% (w/w) ZnCl 2 . The first time the solid residues derived from the production process of XOS were used as the precursor for the co-production of activated carbons (AC). The maximum iodine values and BET surface area were 5623.94 mg/g and 1244.46 m 2 /g, respectively, using 2.20 M ZnCl 2 as the activating agent .
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Molecular Catalysis
·
2017
·
IF 0
Ke Zhao,Shufen Liu,Kexin Li,Ziying Hu,Yukun Yuan,Liushui Yan,Huiqin Guo,Xubiao Luo
Sulfonic acid groups functionalized aromatic carbon microspheres (Ar-CMSs–SO 3 H) were first successfully fabricated directly from waste Camellia oleifera shells by simple steps in the assistance of phloroglucinol . As a heterogeneous solid Brønsted acid catalyst, the as-prepared Ar-CMSs–SO 3 H showed considerably high activity because of its numerous spherical microstructure and abundant surface sulfonic acid groups (–SO 3 H). Subsequently, the as-prepared Ar-CMSs–SO 3 H was applied to the synthesis of biofuels 5-ethoxymethylfurfural (EMF) from biological platform compounds 5-hydroxymethylfurfural (HMF). The catalytic results showed that both sulfonation conditions in the course of Ar-CMSs–SO 3 H preparation and catalytic reaction conditions in the course of EMF synthesis had a significant effect on the EMF yield and HMF conversion rate.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Chunfang Zhu,Meng Zhang,Qiaoling Tang,Qian Yang,Jing Li,Xuan He,Yong Ye
Sapogenin is the main block of Camellia oleifera saponin, which was purified and structurally modified by the C28 acylation reaction to synthesize 19 new derivatives. The growth and biofilm inhibition of Staphylococcus aureus and Escherichia coli was measured to evaluate their antibacterial effects. A three-dimensional quantitative structure–activity relationship (3D-QSAR) assay indicated that the antibacterial activities were significantly enhanced after sapogenin was modified with an aromatic ring or heterocyclic ring and electron-withdrawing substituents at the meta or para position. Among them, the derivative of sapogenin with a 2-mercapto-4-methyl-5-thiazolyl acetyl group obviously destroyed bacterial biofilm and made bacteria lysis. 3D-QSAR provides practical information for the structural design of sapogenin derivatives with strong antibacterial activity, and the C. oleifera sapogenin derivative 28-O-(2-mercapto-4-methyl-5-thiazolyl)-3β,16α,21β,22α-O-tetrahydroxy-oleantel-2-ene-23-aldehyde (S-16) is an effective candidate as an antibacterial agent for the prevention of bacterial resistance against antibiotics.
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APPLIED SURFACE SCIENCE
·
2019
·
IF 5.155
Liping Mei,Huanhuan Qiao,Fei Ke,Chuanyi Peng,Ruyan Hou,Xiaochun Wan,Huimei Cai
Zirconium dioxide-biochar (ZrO 2 /BC) with excellent fluoride adsorption properties, was successfully prepared by calcining the zirconium-impregnated byproduct from Camellia oleifera ( C. oleifera ) seed shell in a one-step method. Compared with C. oleifera seed biochar, the zirconium-impregnated C. oleifera seed shell biochar can effectively adsorb fluoride in water. The adsorption process is consistent with a pseudo second-order kinetic model and the Langmuir adsorption isotherm. Fluoride adsorption was tested in solutions ranging from pH to obtain the best pH value for adsorption. The fluoride adsorption mechanism was better understood by Zeta potential analysis. Thermodynamic studies indicated that the adsorption process was a spontaneous endothermic reaction. Scanning electron microscopy (SEM), Energy-Dispersive Spectroscopy (EDS) and X-ray diffraction (XRD) analyses revealed that the morphology and crystal form of the ZrO 2 /BC were improved and that fluoride could be effectively absorbed. Fourier-transform infrared (FTIR) and X-ray photoelectron spectroscopy (XPS) were used to show how ZrO 2 affects the surface of the zirconium-biochar and to show that the fluoride was removed from the water by ion exchange. This work can not only effectively remove fluoride from the water, but also reuse waste C. oleifera seed to reduce environmental pollution.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Guanfeng Deng,Pingjin Li,Heng Liang,Tao Chen,Lijun Zhou,Hongyu Yang,Xiaoyu Jiang,Chunbang Ding,Shiling Feng
Camellia oleifera leaves were byproduct of the C. oleifera industry which was rich in polysaccharides. Deep eutectic solvent-dual enzyme system (DES-dEAE) was established to achieve the simultaneous hydrolysis reaction of dual enzymes and DES extraction. Currently, there was a lack of research methods that simultaneously deal with the relationship between DES and enzymes and the effect of DES on the polysaccharide extraction rate. In the study, artificial neural network (ANN) and conductor-like screening model for real solvents (COSMO-RS) were used to screen the most suitable DES by predicting the pH and activity coefficient of DESs. Choline chloride and sorbitol constituted the best DES and were used to optimize the process conditions of DES-dEAE by response surface method (RSM). The polysaccharide yield was verified to be 21.46 mg/g with the optimal process conditions: liquid-solid ratio of 33:1, enzyme ratio of 3:1, extraction time of 15 min and water content of 40 %. The C. oleifera polysaccharides obtained by water, DES, and DES-dEAE extractions were observed by scanning electron microscopy and cell wall damage effect of DES-dEAE was confirmed microscopically. The study optimized the complex polysaccharide extraction system using bioinformatics methods, contributing new ideas to the development of simulated extraction.
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PHYTOCHEMICAL ANALYSIS
·
2018
·Vol. 29
·期 6
·639-648
·
IF 2.337
Yaqian Ma,Menghan Liu,Ting Tan,Aiping Yan,Lan Guo,Kun Jiang,Changheng Tan,Yiqun Wan
Introduction Camellia oleifera flowers are rich in flavonoids, but there has been little attention on their application. A simple and reliable method for determining the content of flavonoids in C. oleifera flowers would be very helpful for the utilisation of agriculture resources. Objective To develop an efficient analytical method for the determination of flavonoids in C. oleifera flowers by high-performance liquid chromatography-ultraviolet (HPLC-UV) detection. Methodology Preparing an environmentally-friendly and effective solvent – deep eutectic solvents (DESs) – for compound extraction. Then investigating the influential factors of ultrasound-assisted extraction with DESs by the Box–Behnken design combined with response surface methodology. Results DES-5 synthesised with choline chloride and lactic acid (1:2) acquired excellent extractability for four flavonoids (quercetin 3- O -rhamnoside, kaempferol 3- O -rhamnoside and their aglycones) with different polarity. The proposed method, which could simultaneously determine four flavonoids with HPLC-UV detection for the first time, displays satisfactory recovery yields and high precision with inter-day relative standard deviation lower than 5.80%. Conclusion DESs could be promising solvents for efficiently and selectively extracting bioactive compounds from plant materials, and the analytical method for flavonoids of C. oleifera flowers could provide reference value for its application and be used in other plant resources.
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危险品化学品经营许可证（带存储）