MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Yibo Yan,Ruiqin Zhu,Xin Wang,Cong Hu,Chuixiu Huang,Yan Shi,Hui Yan,Ping Xiang
A multi-structure recognition MIP was synthesized to target 11 synthetic cannabinoids covering six core structural classes. • An integrated MIP-LPME system was developed to overcome matrix interference in blood analysis. • Synergistic enhancement was achieved between MIP and LPME. • Ultrasensitive detection of 11 synthetic cannabinoids in blood was achieved with LOD of 2–4 ng/L. • New strategy for applying hydrophobic functional materials to complex biological matrices was proposed. Synthetic cannabinoids (SCs) pose significant challenges for forensic toxicology due to their diverse core structures and trace levels in blood. Molecularly imprinted polymers (MIPs) exhibit potential for group recognition of structurally diverse subclass of drugs, however, their direct application in blood is severely hindered by matrix interference. Conventional strategies such as sample dilution or surface modification fail to eliminate direct contact between materials and biological matrices. To address these challenges, we prepared a multi-structure recognition MIP (MR-MIP) capable of recognizing 11 SCs representing six core structural classes, and integrated it into the acceptor phase of a hollow fiber liquid-phase microextraction (HF-LPME) system. Kinetics analysis and mass distribution profiling of SCs during extraction illustrated a synergistic enhancement in which LPME protected the MIPs from blood matrix fouling while the MIPs enhanced LPME extraction efficiency through specific adsorption. The MIP-LPME system enabled simultaneous extraction of 11 SCs in whole blood, with limits of detection of 2–4 ng/L and limit of quantification of 4–10 ng/L. To the best of our knowledge, this is the first demonstration of integrating a hydrophobic MIP directly into the LPME acceptor phase for a one-step extraction, which overcomes the poor compatibility between hydrophobic functional materials and the conventional aqueous acceptor phase of LPME through tailored solvent engineering. This synergistic design not only enables highly sensitive and selective trace analysis of structurally diverse SCs in blood, but also provides a strategy for adapting LPME to hydrophobic functional materials, opening new avenues for group selective detection of diverse illicit drugs in complex biological matrices. Download: Download high-res image (152KB) Download: Download full-size image
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高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Lijun Huang,Yuliang Liu,Xingxin Sheng,Yongbo Zhu,Xinyi Li,Meng Liang,Hao Xie,Ming Zhong,Binbin Zhou
A novel MOFs hydrogel composite (MIP-CuMOFs@SA-CS) was created by integrating a molecularly imprinted metal–organic framework (MIP-CuMOFs) within a scaffold of sodium alginate-chitosan (SA-CS(Cu 2+ )). The MIP-CuMOFs@SA-CS served as a recognition element to construct an electrochemical sensor for the detection of trace amounts of chelerythrine (CHE). Reduced graphene oxide (rGO) and reduced graphene nanoribbons (rGNRs) were employed for electrode modification and served as the sensing platform. It was to enhance the stability and sensitivity attributes of the sensor system. The signal probe of copper hexacyanoferrate (CuHCF) was generated through the chemical reaction involving Cu 2+ within the composite structure of MIP-CuMOFs@SA-CS with potassium ferricyanide. The experimental results showed that the constructed sensor has a good linear relationship (50 ∼ 500 nmol L −1 ) and a low detection limit (13.83 nmol L −1 ). Moreover, the sensor exhibited good selectivity, recovery rates, and repeatability (RSD = 2.81 %). With the subjected to spiked serum samples, the demonstrating recovery percentages were in the range of 97.17 % to 101.73 %. The CHE molecularly imprinted electrochemical sensor constructed is capable of detecting trace amounts of CHE with the advantages of simplicity, rapidity, high sensitivity, and low cost, which provides a useful reference for the detection of low concentrations of CHE.
研究领域:
分析化学
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材料科学
·
生物医学工程
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高影响因子
TALANTA
·
2019
·
IF 4.916
Jie Liu,Yuli Wang,Xiaoxue Liu,Qunhui Yuan,Yang Zhang,Yingchun Li
Multiplex electrochemical sensors for amperometric detection of glutathione disulfide (GSSG), glutathione (GSH), cysteine (Cys), cystine (Cyss), β -nicotinamide adenine dinucleotide phosphate (NADP + ) and coenzyme II reduced tetrasodium salt (NADPH) were developed, in which analysis of Cyss, NADP + and NADPH are the first report using this sensing system. Specificity of these sensors were ensured by a layer of molecularly imprinted polymer (MIP) which was electropolymerized in situ with the analytes as template. All the sensors were tested with standard buffers and mouse blood samples, showing satisfactory performance towards the corresponding analytes. Dynamic concentration for the six analytes was in the range of 10 −11 –10 −8 mol/L with the detection limit down to 20 pmol/L. In addition, artificially synthesized MIP film on the electrodes allowed for good selectivity and stability. Real blood sample measurement proved that the sensors owned decent accuracy with recovery value ranging from 92%~112%. More importantly, blood samples from lung cancer patients and healthy donors were assayed by using the proposed sensors. Redox potentials ( E hc) were calculated based on the contents of these endogenic substances, which were utilized to reflect the health status of human body and help diagnose lung cancer. The levels of GSH, NADPH and the absolute value of Ehc (GSH/GSSG) in patients with lung cancer are significantly lower ( P < 0.01) than those in healthy people, while the contents of GSSG ( P < 0.01) are higher. The blood test results suggested that the content of GSH, NADPH, NADP + and Ehc (GSH/GSSG) might serve as biomarkers for lung cancer prediagnosis. These novel sensors for liquid biospy of cancer have cost–benefit and scalability advantage over current techniques, potentially enabling broader clinical access and efficient population screening.
研究领域:
分析化学
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医学
·
生物技术
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PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Ji Yang,Zheng-Zhong Lin,Hui-Ping Zhong,Xiao-Mei Chen,Zhi-Yong Huang
A novel fluorescent probe based on CdTe QDs coated with molecularly imprinted polymers (MIP) was prepared for the determination of leucomalachite green (LMG) with the method of synchronous fluorescence quenching. When the wavelength interval of excitation and emission positions (Δl) was set as 340 nm, the strongest synchronous fluorescence emission of MIP-coated QDs was achieved at 260 nm, enabling the maximum fluorescent quenching of LMG because of the inner filter effect. According to this principle, a synchronous fluorescence quenching method for the rapid detection of LMG in fish was developed. MIP-coated QDs were synthesized using acrylamide (AM) and ethylene glycol dimethacrylate (EGDMA) as functional monomers and cross-linker, respectively. MIP-coated QDs with an average diameter around 67 nm were obtained at the optimum conditions (the molar ratio of 1:8:20 of LMG, AM and EGDMA, 60 mL of acetonitrile, 28 h of polymerization time). The synchronous fluorescence of MIP-coated QDs was linearly quenched by LMG at concentrations from 0.1 to 20 μmol L −1 . Detection limit was 30 nmol L −1 (3 σ , n = 9) with spiked recoveries from 94.0% to 107.8% in fish samples. The results indicate that the method can be applied to the accurate and rapid detection of LMG residues in fish samples.
研究领域:
分析化学
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食品安全
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环境科学
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高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2023
·
IF 6
Hai Chi,Guoqin Liu
Aflatoxin B1 (AFB1) is a toxic metabolite produced by fungi with substantial environmental and health concerns. Thus, the detection of AFB1 has attracted increasing attention. In this study, a fluorometric sandwich biosensor was developed for the detection of AFB1, which was prepared by combining molecularly imprinted polymer (MIP) with an aptamer. Porous carbon (PC) generated by the carbonization of ZIF-8 provided abundant sites for the MIP layer to achieve high-performance recognition of AFB1. The aptamer-modified CdTe/ZnS achieved efficient sandwich analysis of AFB1 as a signal probe. The sandwich biosensor exhibited excellent selectivity and high sensitivity with a dynamic linear range of 0.01–20 ng/mL and good reliability in spiked edible oil samples as low as 4.0 pg/mL. In sum, the developed sandwich biosensor is promising for the detection of AFB1 in food and the environment, with great potential in the control of other challenging food hazards .
研究领域:
分析化学
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食品科学
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环境科学
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高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Zijun Niu,Yingkun Shi,Sijia Liu,Yu Lv,Songlei Wang
In this study, a new electrochemical sensing platform for sulfamethazine (SDM) was prepared using MIP/CNT/MoS 2 -CoNi/GCE as an electrode modifier on a glassy carbon electrode (GCE). Firstly, theoretical simulations and quantum calculations based on density-functional theory (DFT) accelerate the prediction of the template molecule-to-functional monomer ratio. Nanocatalytic materials (CNT/MoS 2 -CoNi) loaded with metal ions Co and Ni were synthesised by a simple solvothermal method and then drop-coated onto GCE. The molecularly imprinted polymer (MIP) film with specific recognition sites can be generated on the modified electrode by electropolymerization. The successful formation of nanocatalytic materials was assessed by various characterisations such as scanning electron microscopy and X-ray diffraction. Under optimal conditions, the constructed sensor showed excellent detection efficiency for the oxidation of SDM in phosphate buffer solution (PBS, pH = 8.0) with a linear range of 0.1 μM to 800 μM and a detection limit of 0.033 μM. The sensor exhibits exceptional stability, reproducibility, and anti-interference capabilities, rendering it suitable for the detection of SDM residues in meat.
研究领域:
分析化学
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材料科学
·
电化学
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高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Qi Wang,Xiaoxia Bai,Fei Liu,Peipei Li,Qianlin Tang
Early diagnosis of cancer is crucial to effective treatment of the patients and to higher survival rates. Tumor marker detection as an effective and noninvasive cancer diagnosis method has important clinical significance. N 1 , N 12 -diacetylspermine (DAS) is a new tumor marker. The development of its detection methods is an urgent need for medical diagnosis and human health monitoring. The conventional detection methods of DAS are labor-intensive and time-consuming approaches. In this work, a novel electrochemical detection platform was established by integrating an organic electrochemical transistor (OECT) with a microfluidic chip and applied for trace measurement of DAS. The sensing unit is a gate electrode in the OECT modified with molecularly imprinted polymers (MIPs), which can be used as an artificial receptor to realize label-free detection. The high specific recognition ability of MIPs films combined with the amplified function of an OECT yielded a highly sensitive and selective electrochemical detection chip. The integrated microfluidic channel controlled the exact amount of solution to be delivered and provided real-time detection of DAS. The experimental results indicated that changes in channel current was proportional to DAS concentrations from 1 nM to 10 μM, with the detection limits of 0.8 nM. It is the first demonstration of the integration of a microfluidic system with a MIP-gated OECT for real-time tumor marker detection. The study results of this work helped to develop Point-Of-Care (POC) tools for the early diagnosis of lung cancer, colon cancer and breast cancer.
研究领域:
生物医学工程
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分析化学
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癌症诊断
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高影响因子
LUMINESCENCE
·
2021
·Vol. 37
·期 2
·348-356
·
IF 2.464
Shilin Shen,Zerong Long,Yi Lu,Jia Chen
A novel double-windows fluorescence sensor for carbofuran (CF) detection was successfully developed based on rare-earth Eu,Tb-doped Y 2 O 3 @SiO 2 -based molecularly imprinted nanoparticles (MINs) with a multilayer core-shell structure. The recognition process of the MINs for CF was fairly fast and needed only ~8 min to reach a dynamic equilibrium. Interestingly, one fluorescence attenuation window was found with an increase in CF concentration ( Q ) from 0.1 to 10 μg ml −1 and with a limit of detection (LOD) of 0.04 μg ml −1 at 544 nm belonging to the Tb 3+ emission, as well as another fluorescence enhanced window within the CF concentration range 10–100 μg ml −1 (LOD = 4 μg ml −1 ) at 617 nm of Eu 3+ emission in the dispersed rare-earth-doped MIN colloidal aqueous solution. Luminescence resonance energy transfer from CF to Eu 3+ and an inner filter effect of CF towards Tb 3+ , as well from the two independent detection windows were clearly observed simultaneously. The competition experiment displayed hardly any marked interference during detection of CF following addition of its analogues (carbaryl, isoprocarb, aldicarb, methomyl, and etofenprox). Moreover, the MINs could also be applied to accurately detect CF in rhubarb and wolfberry samples with recoveries of 85.7–92.2%. This sensing system has high specific recognition and a wide detection range for CF and provides new opportunities for pesticide detection.
研究领域:
分析化学
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纳米技术
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环境科学
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农药检测
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PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 6
·1-10
·
IF 5.3
Zhong Yujun,Li Zhiyang,Zhang Anlin,Peng Yuqing,Zhou Hao,Wang Bin,Xie Lianwu,Guo Yaping
A novel electrochemical sensor, MIP/Cu-MOF/rGO/AuNPs/GCE, was developed by depositing gold nanoparticles, coating Cu-MOF/GO on the surface of glassy carbon electrode (GCE) before electroreducing graphene oxide (GO) to rGO and covering molecularly imprinted membrane by electropolymerization for highly sensitive detection of electroneutral organophosphorus pesticide residues in agricultural product. Cyclic voltammetry, differential pulse voltametry, scanning electron microscopy, energy-dispersive spectroscopy, and atomic force microscopy were used to characterize the imprinted sensor. Several key factors such as chitosan concentration, suspension volume, pH of polymerization solution, and polymerization scanning rate during preparation of the imprinted sensor were optimized in detail. When electroneutral phosmet was used as a template, the linear range of MIP/Cu-MOF/rGO/AuNPs/GCE for detecting phosmet was 1.00 × 10 −14 –5.00 × 10 −7 mol/L with the limit of detection of 7.20 × 10 −15 mol/L at working potentials of − 0.2 to 0.6 V. The selectivity, reproducibility, and repeatability of MIP/Cu-MOF/rGO/AuNPs/GCE were all acceptable. The recoveries of this method for determining phosmet in real samples ranged from 94.2 to 106.5%. The MIP/Cu-MOF/rGO/AuNPs/GCE sensor could be applied to detect electroneutral pesticide residues in organisms and agricultural products. Graphical Abstract
研究领域:
分析化学
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环境科学
·
农业科学
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PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 187
·期 1
·1-10
·
IF 5.479
Li Yue,Wang Yan,Wang Mingchao,Zhang Jinyue,Wang Qingwei,Li Hongji
In a new method for the determination of trace levels of chlorophenols in river waters, detection via surface-enhanced Raman scattering (SERS) is combined with recognition by a molecularly imprinted polymer (MIP). Nanoparticles of type Cu 2 O@Ag were synthesized by attaching silver particles to the surface of Cu 2 O nanoparticles. The Cu 2 O@Ag were then coated with a layer of a MIP that was obtained by atom transfer radical polymerization using from methacrylic acid as monomers and 2,6-dichlorophenol as the template. The morphology of Cu 2 O is found to be flower-like. The Cu 2 O@Ag-MIPs displays a strong SERS effect. Following removal of the template by rinsing with the mixture solution of methanol/acetic acid (9/1, v /v), the material was used to selectively bind 2,6-dichlorophenol, 2,4-dichlorophenol and 2,4-dichlorophenol. The SERS peak intensity at 1580 cm −1 increases linearly with the concentration of the various chlorophenols in the range from 10 nM to 1 mM, and the detection limit is 5.8 nM. The imprinting factor is 4.62. The method was applied to the analysis of (spiked) river water, with recoveries ranging from 91.8 to 115.4% and relative standard deviations of <4.5%.
Graphical abstract
研究领域:
环境科学
·
分析化学
·
纳米技术
·
材料科学
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PubMed
高影响因子
JOURNAL OF POROUS MATERIALS
·
2023
·Vol. 30
·期 6
·2129-2137
·
IF 2.6
Tao Ruyu,Liu Hao,Xiang Pan,Lin Yichao,Zhou Junjie,Zhou Chen,Zhao Yayun,Tian Ziqi,Zhang Qiuju
Nanoscale Zr-based metal-organic framework (MIP-202) particles were successfully prepared via a seeds-assisted hydrothermal secondary synthesis. The uniformly-shaped MIP-202 particles exhibit excellent performance on selective CO 2 adsorption from CO 2 /CH 4 and CO 2 /N 2 mixtures. At 298 K and 1 bar, the uptake ratios of CO 2 /CH 4 and CO 2 /N 2 of MIP-202 particles are as high as 32.6 and 65.2, respectively. The IAST (ideal adsorbed solution theory)-predicted selectivities of CO 2 /N 2 (50/50, v/v) and CO 2 /CH 4 (50/50, v/v) reach to 4.5 × 10 10 and 102.2, respectively. The breakthrough experiments further demonstrate that the CO 2 /CH 4 and CO 2 /N 2 mixtures can be efficiently separated through an adsorption column packed with MIP-202 particles. In addition, the as-prepared MIP-202 particles had a low iso-enthalpy of adsorption of CO 2 of 32.95 kJ/mol, which is favorable for the regeneration of the adsorbent. Herein the as-prepared MIP-202 is a potential material for efficient separation of CO 2 from CH 4 or N 2 through an energy- and cost-saving CO 2 capture process.
研究领域:
材料科学
·
环境化学
·
能源工程
·
纳米技术
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DOI
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Wenyan Yan,Shuyue Li,Baoqing Bai,Ying Zhang,Jinhua Zhang,Tao Bo,Qinghua Wang,Ying Gu,Yukun Yang,Shuo Wang
A MIP-modified MOF/COF hybrid nanoplatform for PEC sensing, adsorption, and photocatalytic degradation was developed. • The MOF/COF hybrid enhanced photocurrent through improved light absorption and charge separation. • The MIP layer provided high selectivity. • The platform enabled ultrasensitive PAT detection and efficient adsorption-photocatalytic degradation. Rational design of photoactive materials is essential for the sensitive detection and efficient removal of hazardous contaminants. Herein, a visible-light-responsive covalent organic framework/metal-organic framework (COF/MOF) hybrid with uniform COF layers grown in-situ on the MOF surface, followed by the introduction of a molecularly imprinted polymer (MIP) layer via a sol-gel process, was constructed for the PEC sensing, adsorption, and photocatalytic degradation of patulin. The hybrid architecture enhanced photoelectrochemical performance by facilitating charge separation and improving light harvesting. The resulting PEC sensor exhibited a wide linear range (0.1 nM-1 mM) and a low detection limit (0.055 nM), along with good reproducibility and stability in real samples. Moreover, the MIP/MOF/COF hybrid enabled efficient adsorption and photocatalytic degradation of patulin, driven by photogenerated holes and superoxide radicals. This work provides a promising strategy for the efficient and highly sensitive detection and removal of contaminants. Download: Download high-res image (236KB) Download: Download full-size image
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高影响因子
危险品化学品经营许可证（带存储）