RSC Advances
·
2018
·Vol. 8
·期 53
·30512-30519
·
IF 2.936
Jian-Xiu Li,Yan-Yan Huang,Xian-Rui Chen,Qi-Shi Du,Jian-Zong Meng,Neng-Zhong Xie,Ri-Bo Huang
Acetoin is an important platform chemical with a variety of applications in foods, cosmetics, chemical synthesis, and especially in the asymmetric synthesis of optically active pharmaceuticals. It is also a useful breath biomarker for early lung cancer diagnosis. In order to enhance production of optical (S)-acetoin and facilitate this building block for a series of chiral pharmaceuticals derivatives, we have developed a systematic approach using in situ-NADH regeneration systems and promising diacetyl reductase. Under optimal conditions, we have obtained 52.9 g L−1 of (S)-acetoin with an enantiomeric purity of 99.5% and a productivity of 6.2 g (L h)−1. The results reported in this study demonstrated that the production of (S)-acetoin could be effectively improved through the engineering of cofactor regeneration with promising diacetyl reductase. The systematic approach developed in this study could also be applied to synthesize other optically active α-hydroxy ketones, which may provide valuable benefits for the study of drug development.
研究领域:
生物催化
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药物化学
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代谢工程
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生物技术
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产品关联
DOI
PubMed
高影响因子
IONICS
·
2016
·Vol. 22
·期 11
·2225-2233
·
IF 2.119
Lu Juanjuan,Liu Yuelin,Liu Xinhe,Lu Xiaoquan,Liu Xiuhui
In this article, we constructed an electrochemical sensor for highly sensitive determination of β-nicotinamide adenine dinucleotide (NADH) using the poly(diallyldimethylammonium chloride)-functionalized reduced graphene oxide nanocomposite modified electrode (PDDA-rGO/GCE). The nanocomposite was characterized by transmission electron microscopy, Fourier transform-infrared spectrometry, ultraviolet-visible absorption spectrometry, cyclic voltammetry, and electrochemical impedance spectroscopy. The experimental results demonstrated that the fabricated sensor exhibited excellent electrocatalytic activity on the oxidation of NADH with a decreased about 200 mV in overpotential and 5.0-fold increment in the oxidation peak current compared to a bare GCE. Applying cyclic voltammetry, a wide linear range from 1.0 × 10 −7 to 2.9 × 10 −3 M with low limit of detection (LOD) of 3.4 × 10 −8 M and high sensitivity of 463.3 μA mM −1 cm −2 were obtained, which was superior to other NADH electrochemical sensors reported previously. Furthermore, the fabricated NADH sensor possessed excellent selectivity, reproducibility, and stability. The oxidation mechanism of NADH at the PDDA-rGO/GCE was also discussed in detail.
A simple NADH sensing platform based on PDDA-rGO composite is constructed, which exhibits an ultra-low detection limit ( 3.4×10-8 M) towards the oxidation of NADH, and the electrochemical reaction mechanism of NADH is discussed in detail.
研究领域:
生物电化学
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生物传感器
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纳米生物技术
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分子诊断
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转化医学
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电分析化学
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DOI
高影响因子
ChemPhotoChem
·
2022
·Vol. 6
·期 8
·e202200067
·
IF 3.679
Yaoqiang Wang,Yu Jin,Zishuai Wang,Gang Xiao,Haijia Su
Graphical Cascading : A two-pot, light-dark cascade NADH regeneration procedure was developed. First, g-C 3 N 4 nanosheets photocatalyzed the conversion of triethanolamine (TEOA) to glycolaldehyde, which reduced NAD+ to NADH in the dark reaction step, thus avoiding the photo-deactivation of NADH. Photocatalytic regeneration of NAD(P)H is a green and promising method which utilizes clean solar energy. However, we found in this study that light exposure of NADH will cause the deactivation of the regenerated NADH. Therefore, we used ultrathin g-C 3 N 4 nanosheets as photocatalyst and developed a two-pot light-dark cascade procedure for NADH regeneration to avoid the light exposure and the deactivation of NADH. This two-pot procedure included a first light reaction step where triethanolamine was converted to glycolaldehyde through visible light photocatalysis which could reduce NAD + to NADH through a non-catalytic process in the followed dark reaction step. Using this procedure, the NADH productivity reached 1.625 mmol ⋅ h −1 ⋅ g −1 and was about 29 times higher that obtained in the one-pot method. The regenerated NADH using the two-pot procedure could act as cofactor of alcohol dehydrogenase to catalyze the reduction of acetaldehyde, illustrating the application potential of this procedure to aid enzyme catalyzed redox reactions.
研究领域:
化学
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材料科学
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生物技术
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环境科学
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DOI
高影响因子
Molecular Catalysis
·
2023
·
IF 4.6
Bing-Yi Yang,Zhi-Neng You,Jiang-Tao Xue,Jiang Pan,Chun-Xiu Li,Jian-He Xu
7β-Hydroxysteroid dehydrogenases (7β-HSDHs) play an important role in the enzymatic synthesis of ursodeoxycholic acid (UDCA), which is a value-added compound with a range of pharmacological activities. Since the cofactor NADH is much cheaper than NADPH, the production of UDCA using an NADH-dependent 7β-HSDH has better prospects than that using an NADPH-dependent 7β-HSDH. However, a major bottleneck in UDCA biosynthesis is the poor catalytic activity of the current NADH-dependent 7β-HSDHs. In this work, a new and natively NADH-dependent Rs 7β-HSDH from Roseococcus sp. was identified, which showed a high specific activity of 63.3 U mg −1 protein toward 7-oxo-lithocholic acid, with a catalytic efficiency ( k cat / K M ) of 515 mM –1 s –1 . In a preparative biotransformation (100-mL scale) using Rs 7β-HSDH and an NAD + -dependent 7α-HSDH, with O 2 /NOX and HCOO − /FDH systems for the regeneration of cofactors (NAD + and NADH), 25 mM chenodeoxycholic acid was completely converted to UDCA in one-pot two-step cascade, with an 80% isolated yield and a total turnover number (TTN) of 6586 for Rs 7β-HSDH. The environmental factor ( E -factor) of this process was 8.37 when water was excluded, much lower than those obtained from other processes reported so far, indicating a great potential of this Rs 7β-HSDH for more sustainable and cleaner enzymatic production of UDCA.
研究领域:
生物技术
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药物化学
·
酶学
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产品关联
DOI
高影响因子
AUSTRALIAN JOURNAL OF CHEMISTRY
·
2021
·
IF 1.321
Yuqin Liao,Ruiyun You,Min Fan,Shangyuan Feng,Dechan Lu,Yudong Lu,Yuqin Liao,Ruiyun You,Min Fan,Shangyuan Feng,Dechan Lu,Yudong Lu
Nicotinamide adenine dinucleotide (NADH) is an important coenzyme involved in various metabolic processes of living cells. As an important biomarker, NADH is associated with breast cancer and Alzheimer’s disease. In this paper, silver plated gold core–shell nanoparticles containing Raman signal molecules were synthesised on the basis of bare gold. Using the Raman peak corresponding to the 4-mercaptobenzonitrile (MB) silent region C≡N vibration for quantification, while avoiding competition with the precious metal surface binding site to be measured, it can also be free from the interference of endogenous biomolecules. On the one hand, it can correct the working curve, on the other hand, it can avoid competing with the binding site. Compared with the core–shell structure prepared here, the limit of detection (LOD) for NADH was only 10−5 M for bare gold and the LOD for the core–shell structure prepared on the basis of bare gold was 3.3 × 10−7 M. In terms of correction, with Rhodamine 6G (R6G) as a Raman signalling molecule, the R2 value before SERS detection and correction is only 0.9405, and the R2 value after correction increases to 0.9853. The unique fingerprint peak of SERS was used to realise the quantitative detection of NADH, which realizes the detection of NADH in complex biological samples of serum and provides the possibility for expanding the early diagnosis of breast cancer.
研究领域:
分析化学
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生物医学工程
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癌症研究
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神经退行性疾病
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2011
·Vol. 174
·期 1
·31-39
·
IF 2.578
Cui Lin,Ai Shiyun,Shang Kun,Meng Xiaomeng,Wang Chengcheng
We have prepared a glassy carbon electrode modified with poly-2,6-pyridinedicarboxylic acid and with magnetic Fe 3 O 4 nanoparticles. This modification enhances the effective surface area and the electrocatalytic oxidation of nicotinamide adenine dinucleotide (NADH) in addition to providing positively charged groups for electrostatic assembly of the phosphate group of NADH. The modified electrode responds linearly to NADH in the range from 5 × 10 −8 to 2.5 × 10 −5 M and gives a lower detection limit of 1 × 10 −8 M. It displays satisfactory selectivity and reproducibility. The sensor was applied to rapid screening of plant extracts for their antioxidant properties.
Poly-2,6-pyridinedicarboxylic acid (PDC) was fabricated by electropolymerizing 2,6-pyridinedicarboxylic acid with cyclic voltammetry (CV) on the glassy carbon electrode (GCE) surface. The magnetic Fe 3 O 4 nanoparticles treated with aminopropyltriethoxysilane (APTS) modified on the PDC/GCE to form APTS-Fe 3 O 4 /PDC composite film. The APTS-Fe 3 O 4 /PDC film had enhanced the effective electrode surface area and provided positively charged groups for electrostatic assembly of phosphate group of NADH.
研究领域:
分析化学
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生物电化学
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传感器技术
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产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
·
2017
·Vol. 92
·期 9
·2477-2487
·
IF 3.135
Niyomukiza Samuel,Teng Bao,Xian Zhang,Taowei Yang,Meijuan Xu,Xin Li,Irene Komera,Tuyishime Philibert,Zhiming Rao
BACKGROUND 2,3-Butanediol (2,3-BD) has a wide range of applications in chiral molecular synthesis, biofuel additives, and in food flavor additive manufacturing. Fermentation is a favorable method for 2,3-BD production. However, it requires much time and produces several NADH related byproducts which compete with 2,3-BD production. Bacillus subtilis has an excellent ability for 2,3-BD production by biocatalysis. However, its production is limited by low intracellular NADH and the reversible property of acetoin reductase (AR/2,3-BDH). The whole cell biocatalyst process with two different NADH regeneration systems was designed for efficient production of 2,3-BD in B. subtilis 168. RESULTS Formate dehydrogenase and glucose dehydrogenase for NADH regeneration were successfully co-expressed with acetoin reductase in B. subtilis 168. After optimization of biocatalyst bioconversion conditions, B. subtilis 168/pMA5- bdhA-Hpa II- fdh yielded 74.5 g L −1 of 2, 3-BD with 9.3 g L −1 h −1 productivity by fed batch and 115.4 g of 2,3-BD was achieved using same batch bacterium by three repeated batch bioconversions. On the other hand, 63.7 g L −1 of 2, 3-BD was produced with 7.92 g L −1 h −1 productivity by B. subtilis 168/pMA5- bdhA-Hpa II- gdh . To our knowledge, the volume productivity obtained here is the highest ever reported for biocatalysis. CONCLUSION A higher productivity of 2,3-BD from acetoin was achieved by whole cell biocatalysis with NADH regeneration systems in B. subtilis 168. This approach can be applied for NADH related bio-based chemicals production to improve titer, yield and productivity. © 2017 Society of Chemical Industry
研究领域:
生物化学
·
微生物学
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代谢工程
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DOI
高影响因子
Molecular Catalysis
·
2024
·
IF 3.9
Xuecai Wei,Xiaoyang Yue,Quan Yuan,Ying He,Li Ma,Liya Zhou,Guanhua Liu,Yunting Liu,Yanjun Jiang
Ethyl ( S )-4‑chloro-3-hydroxybutyrate [( S )-CHBE] is attractive as a necessary intermediate for the production of highly valued products in pharmaceutical industries. Nowadays, alcohol dehydrogenases play an increasingly essential role in the asymmetric synthesis of chiral alcohols. In this work, a nicotinamide adenine dinucleotide (NADH)-dependent alcohol dehydrogenase from Pseudomonas bacterium ( Pb ADH) was obtained by genome mining method and identified to exhibit an excellent tolerance against the high substrate and product concentrations. For the regeneration of NADH, Bacillus megaterium glucose dehydrogenase ( Bm GDH) was successfully co-expressed with Pb ADH in E. coli cells. So much as 3.5 M (574 g/L) ethyl 4-chloroacetoacetate (COBE) can be converted to ( S )-CHBE completely with a > 99 % ee value under the catalysis by recombinant cells, even in a monophasic aqueous media with a single batch charge of substrate. In addition, in order to verify the substrate scope of Pb ADH, six other α/β-ketone esters and aromatic ketones were also evaluated as substrates at high substrate concentrations (100–650 g/L), and the corresponding chiral alcohols with highly optical purity were also achieved by co-expressed E. coli cells.
研究领域:
生物化学
·
酶工程
·
制药工业
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产品关联
DOI
高影响因子
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2017
·Vol. 19
·期 33
·22472-22481
·
IF 4.123
Jiying Liang,Xue Yu,Tiangang Yang,Menglu Li,Li Shen,Yue Jin,Hongyun Liu
In this paper, poly(N-isopropylacrylamide-co-3-aminophenylboronic acid) (P(NIPAM-co-APBA)) copolymer films were successfully electropolymerized on the Au electrode surface. The electroactive probe ferrocene carboxylic acid (FCA) in solution showed reversible thermal-, glucose- and pH-responsive on–off cyclic voltammetric (CV) behaviors at the film electrodes. The comparative experiments demonstrated that the thermo-responsive property of the film electrode was ascribed to the PNIPAM component of the films, whereas the glucose- and pH-sensitive behaviors came from the PAPBA constituent. The reduced form of nicotinamide adenine dinucleotide (NADH) could be electrocatalytically oxidized by FCA at the film electrodes, which would greatly amplify the multi-responsive CV signal difference between the on and off states. On the basis of these results, a binary 4-input/4-output logic circuit was fabricated with temperature, glucose, pH and NADH as inputs and the CV responses at 4 different levels as outputs. Moreover, a ternary CONSENSUS logic circuit was established on the same platform, which was the first report on the combination of ternary logic gate and bioelectrocatalysis without using enzymes. This work provided a novel idea for constructing complicated biocomputing systems by increasing the number of inputs/outputs with multi-sensitive interfaces and by designing new types of multi-valued logic gates on the basis of bioelectrocatalysis.
研究领域:
生物计算系统
·
生物电化学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Jia Chen,Li Wang,Yanni Huang,Zhan Li,Haijuan Zhang,Mohammad Chand Ali,Juewen Liu,Xingguo Chen,Hongdeng Qiu
The exploration of novel nanocomposite about two-dimensional (2D) nanoporous graphene (NPG) is of great significance for the further investigation of their outstanding properties and potential applications. Thus, an exceptionally facile and rapid strategy named combustion-reduction synthesis is reported. 2D NPG/cuprous oxide (NPG/Cu 2 O) nanocomposite can be obtained via combustion of graphene oxide (GO) and copper chloride in an oven at 450 °C for only one minute. By simply washing away Cu 2 O with acid, NPG was obtained. By burning away graphene, Cu 2 O nanoparticles were also obtained. As a new kind of hybrid materials, NPG/Cu 2 O nanocomposite presented much more excellent catalytic activity than the reduced GO/Cu 2 O nanocomposite, free GO, NPG and Cu 2 O nanoparticles. The outstanding catalytic property can be ascribed to the hybridization of Cu 2 O and 2D NPG architectures. With this new hybrid material, a chemiluminescence sensing platform was firstly constructed for fast, excellently sensitive and highly selective determination of nicotinamide adenine dinucleotide (NADH) with a detection limit of 3.5 nM. The as-established approach was successfully applied for quantitative determination of NADH in human serum and cell lysates. This strategy may open up a valuable avenue to fabricate a diversity range of nanocomposites containing nanoporous graphene and metal oxides with various potential applications.
研究领域:
材料科学
·
纳米技术
·
分析化学
·
生物传感器
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2024
·
IF 10.7
Sile Deng,Xiaoju Men,Muhua Hu,Xiao Liang,Yujuan Dai,Zhengkun Zhan,Zhongchao Huang,Haobin Chen,Zhuxin Dong
Reduced nicotinamide adenine dinucleotide (NADH) has a strong impact on physiological metabolism, and its concentration is related to metabolic and neurodegenerative diseases. A more reliable and accurate detection method for NADH quantitation is needed for early disease diagnosis and point-of-care testing. Aggregation-induced emission (AIE) materials are widely used to improve the sensitivity in analytes assays due to their anti-aggregation-caused quenching property. Here we developed TPA-BQD-Py AIE-dots transducers and evaluated its performance in NADH detection. The NADH concentration-dependent ratiometric sensing was based on electron transfer from TPA-BQD-Py AIE-dots to NADH with variable fluorescence intensity at 584 nm and 470 nm, resulting in high sensitivity (limit of detection at 110 nM), photostability, selectivity, and a rapid and reversible response. We further developed the application of TPA-BQD-Py AIE-dots transducers in in vivo NADH imaging using a smartphone and digital camera, respectively, demonstrating the potential for NADH point-of-care testing.
研究领域:
分析化学
·
生物医学工程
·
即时诊断
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荧光传感
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代谢组学
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产品关联
DOI
PubMed
高影响因子
BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2021
·Vol. 45
·期 2
·353-364
·
IF 3.21
Li Xue-Yong,Xu Meng-Qiu,Liu Hui,Zhou Qiang,Gao Jian,Zhang Ye-Wang
The combined cross-linked enzyme aggregates (combi-CLEAs) containing galactitol dehydrogenase (Gdh) and NADH oxidase (Nox) were prepared for l -tagatose synthesis. To prevent the excess consumption of cofactor, Nox in the combi-CLEAs was used to in situ regenerate NAD + . In the immobilization process, ammonia sulfate and glutaraldehyde were used as the precipitant and cross-linking reagent, respectively. The preparation conditions were optimized as follows: 60% ammonium sulfate, 1:1 (molar ratio) of Gdh to Nox, 20:1 (molar ratio) of protein to glutaraldehyde, and 6 h of cross-linking time at 35 °C. Under these conditions, the activity of the combi-CLEAs was 210 U g −1 . The combi-CLEAs exhibited higher thermostability and preserved 51.5% of the original activity after eight cycles of reuses at 45 °C. The combi-CLEAs were utilized for the preparation of l -tagatose without by-products. Therefore, the combi-CLEAs have the industrial potential for the bioconversion of galactitol to l -tagatose. Graphical abstract
研究领域:
生物技术
·
酶学
·
化学工程
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）