Biofuels Bioproducts & Biorefining-Biofpr
·
2024
·
IF 3.2
Yue Ouyang,Ying Liu,Longjin Tian,Yongyu Ding,Guozhi Fan,Cheng Pan,Qunpeng Cheng
Anaerobic digestion is a promising technology for the treatment of food waste (FW) but it requires optimization to improve its performance. This study investigated whether combining the addition of biochar with lipase pretreatment could improve the anaerobic digestion of FW. The results showed that biochar stimulated the release of soluble substances, resulting in an increase in the methane yield of groups with added biochar by 16.24% to 19.36% in comparison with the group without biochar. Lipase pretreatment substantially shortened the fermentation time required to complete the anaerobic digestion, from 15 to 9 days. Lipase pretreatment further improved microbial abundance and promoted the enrichment of functional microbes in the anaerobic digestion of FW mediated by biochar. It also changed the methane production pathway from acetotrophic to hydrogenotrophic, thereby ensuring the stability of the anaerobic digestion system in the presence of a high ammonia nitrogen concentration.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULAR CRYSTALS AND LIQUID CRYSTALS
·
2023
·
IF 0.7
Zihao Meng,Jian Wu,Qingyun Li,Youyan Liu,Aixing Tang
Lipase OF from Candida cylindracea was adsorbed onto surfactant-modified Na-bentonite (Bent), and the effects of the surfactants on the catalytic properties of immobilized lipase were studied in detail. The results showed that both the specific activity and enzyme loading of immobilized lipase varied significantly with modified support of different surfactants. The highest specific activity was obtained with Tween20-modified Bent: 11.9-fold higher than the non-modified sample. Upon modification by a single surfactant, two kinds of surfactants were combined to process the modification. It was surprising to find that the enzyme immobilized on CTAB (60%) and TX-100 (40%) co-modified Bent yielded a specific activity recovery of 133.4% indicating that the enzyme might be “activated”. Kinetic analyses for the hydrolysis of p-nitrophenyl palmitate (p-NPP) showed that Km decreased from 5.57 mg mL−1 of free lipase to 3.43 mg·mL−1 and Vmax increased 1.35-fold higher. The structure, surface morphology, and hydrophobicity of the modified bentonite and immobilize lipase were characterized via X-ray diffraction, Fourier transform-infrared spectroscopy, scanning electron microscopy, and contact angle measurements. We assumed that lipase molecules may be bound to the surface of the carrier, and the surface hydrophobicity and the change of the functional group caused by the modification of surfactant may be the key factor affecting lipase immobilization.
研究领域:
生物化学
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酶工程
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产品关联
DOI
高影响因子
Journal of Materials Chemistry A
·
2014
·Vol. 2
·期 43
·18339-18344
·
IF 0
Zhiming Chen,Wenhui Xu,Li Jin,Juanjuan Zha,Tingxian Tao,Ying Lin,Zhilin Wang
Amine-functionalized Fe3O4@C nanoparticles have been successfully synthesized on a large scale via a solvothermal route. The morphology, structure and properties of the Fe3O4@C nanoparticles were investigated through different analytical tools. Due to the magnetic nature and the presence of amine-functionalized groups, the as-prepared Fe3O4@C nanoparticles were employed as magnetic carriers for lipase immobilization. Our results indicate that the lipase loading amount on the magnetic Fe3O4@C nanoparticles was about 115.6 mg of protein per g. The factors related to the catalytic activity of the immobilized lipase on the magnetic carriers were systematically investigated. Hydrolytic activity tests reveal that the immobilized lipase retains about 93% of the free enzyme activity. It should be mentioned that the thermal stability of the immobilized lipase was strikingly higher than that of the free lipase. Most importantly, the immobilized lipase retained more than 68% of its initial activities after 10 times reuse. It is hoped that the amine-functionalized Fe3O4@C nanoparticles may find an application in biotechnology and catalysis.
研究领域:
生物技术
·
催化科学
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产品关联
DOI
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2022
·Vol. 194
·期 11
·5119-5131
·
IF 3.094
Yang Yang,Wang Jia,Yang Bo,Lan Dongming,Wang Yonghua
The amino acid residues lining the substrate binding pocket play quite an important role during the lipase catalytic process. The conversion of those residues might cause a dramatic change in the lipase properties, such as the substrate selectivity of lipase. In our study, T237 residue sitting on the entrance of the catalytic pocket in lipase MAS1 was important for the catalytic performance. When replacing polar Thr with the positively charged Arg, the synthesis ratio of partial glycerides/triglycerides increases to 6.32 rather than 1.21 of MAS1 wild type (WT), as the substrate ratio of glycerol and fatty acids is 1:3. And the fatty acid preference shifted to long-chain substrates for mutant T237R rather than middle-chain substrates for MAS1 WT. Molecular docking analysis revealed that hydrophobic and side chain properties of Arg might contribute to the change of the MAS1 lipase catalytic performance. This work would pave a way for the accurate rational transformation of the lipases to produce value lipid for industrial application. Graphical abstract
研究领域:
生物化学
·
酶学
·
生物技术
·
蛋白质工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2023
·
IF 4.1
Hong Zhang,Xin Zhang,Lei Wang,Bo Wang,Xu Zeng,Bo Ren,Xiaodong Yang
A novel lignin-enhanced graphene aerogel (LGA) was prepared by one-step hydrothermal synthesis, and lipase from Pseudomonas sp. (PSL) was immobilized on LGA successfully by interfacial activation. The catalytic activity and enantioselectivity of LGA-PSL for the preparation of (S)-2-octanol by an enantioselective transesterification were improved obviously. The characterization of LGA and LGA-PSL was performed. X-ray diffraction and Fourier transform infrared spectroscopy demonstrated the formation of numerous electrostatic and hydrogen bonds between lignin and graphene in the aerogel structure. In addition, the specific surface area pore size analyzer (BET) test proved that the introduction of lignin significantly increased the specific surface area and pore size of the aerogel material, which improved the immobilization efficiency of lipase in the aerogel. The introduction of lignin has changed the original lamellar structure of the graphene oxide (GO) aerogels. The lignin cross-linked with the GO lamellae through hydrogen bonding, causing a porous structure to form between the original lamellae, thus increasing their specific surface area. The immobilized lipase (LGA-PSL) was used for the preparation of (S)-2-octanol by an enantioselective transesterification, and the reaction conditions for this enzymatic transesterification had been optimized. LGA-PSL exhibited a high catalytic performance and could be reused four times in this reaction. Based on these results, LGA as an immobilization carrier had potential applications in the industrial application of lipase.
研究领域:
材料科学
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生物催化
·
酶工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2022
·
IF 6.449
Shuai Wang,Yunxiao Wang,Shunxin Wang,Shuang Guo,Dongyu Gu,Jifeng Wang,Yi Yang
In this study, R. pseudoacacia flowers exhibited potential lipase ( Aspergillus niger ) inhibitory activity. To quickly explore the active compound, ligand fishing was adopted for screening the ligand from R. pseudoacacia flowers by lipase immobilized on magnetic polyamidoamine dendrimer-coated multi-walled carbon nanotubes . After ligand fishing, the target compound was separated purposefully by optimizing the separation conditions of high-speed counter-current chromatography (HSCCC). As results, kaempferol with the IC 50 of 327.64 ± 15.33 μg/mL was separated and identified. The further molecular docking results indicated that kaempferol was interacted with amino acid residue in the active site of lipase through van der Waals force, hydrogen bonds and Pi-Pi T-shaped interactions, yielding the binding energy of − 6.43 kcal/mol. The present method not only revealed the lipase inhibitory substances in R. pseudoacacia , but also provided an effective means for the screening of other natural substances.
研究领域:
生物化学
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纳米技术
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药理学
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植物科学
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Suo Wang,Yuan Ma,Yao Li,Xuan Ji,Xu Fei,Longquan Xu,Yi Wang,Jing Tian
It is still a challenge to design a supramolecular interaction system for immobilizing enzymes efficiently and stably. In this work, inspired by octopus suckers, we used cyclodextrin with a similar structure to the octopus sucker cavity as supramolecular interaction functional units of soft tentacles to immobilize lipase through physical adsorption . By modifying the surface of cyclodextrin with polar hydrophilic groups, the difference in hydrophilic and hydrophobic properties and stability within the molecule were effectively improved, thereby enhancing the cavity suction of cyclodextrin. The enzyme load of the soft tentacles reached 10 mg/g, and the lipase was adsorbed firmly under high strength mechanical action (the retention rate is 66.4%, other physical adsorption under the same conditions is 51.6%). The soft tentacle immobilized lipase showed good dispersibility in the organic phase and its water absorption characteristics could provide oil-water interface for lipase in organic phase reaction, promoting the forward transesterification reaction. Compared with free lipase, the soft tentacle immobilized lipase (L-CDST-Ms) showed the improved activity, which could reach 180% of that of free lipase. The L-CDST-Ms was applied to biodiesel production, and the conversion rate could reach 94%. The results showed that the immobilization method opened up a new way for the application of lipase in transesterification .
研究领域:
生物化学
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酶工程
·
可再生能源
✓ 已验证
产品关联
DOI
高影响因子
Journal of Nanoelectronics and Optoelectronics
·
2018
·Vol. 13
·期 7
·1041-1047
·
IF 1.069
Dong Yuhuan,Ma Feng
A type of nanofiber membrane of polyvinyl butyral (PVB)/OP-10/lipase was prepared using electrostatic spinning technology. The results of environmental scanning electron microscopy (ESEM) indicate that the microscopic morphology of PVB/OP-10/lipase membrane spun under 14 kV of voltages,
10 %wt concentration of emulsifier (relative to the amount of PVB) and 10 cm of the distance from the receiver to the needlepoint is uniform. The stability of immobilized and free lipase was studied under different pH, voltages and temperature and storage time. It is showed compared with the
free enzyme, the immobilized enzyme has better temperature-resistant and better storage stability. The immobilized enzyme has recycling activity of 65% after 10 times uses. The esterification rate of lauric acid with lauryl alcohol catalyzed by immobilized lipase can get to 72% after 20 hours
of reaction. While the reaction of lauric acid with lauryl alcohol catalyzed by free lipase cannot occur.
研究领域:
生物化学
·
材料科学
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 10.652
Wei Zhuang,Xuebo Quan,Zhenfu Wang,Wenfeng Zhou,Pengpeng Yang,Lei Ge,Byron Villacorta Hernandez,Jinglan Wu,Ming Li,Jian Zhou,Chenjie Zhu,Hanjie Ying
Compared to the traditional chemical reduction method, a green and efficient strategy was successfully applied to regulate the surface heterogeneity of graphene oxide (GO). In this work, different kinds of amino acids were used to reduce and modify GO. The reduction and adsorption disparities of amino acids contributed to the difference of structural heterogeneity in terms of density, thermal tolerance and disorder of the oxygen-containing groups of GO. The modification also resulted in discrepancies in surface properties of the GO samples, such as hydrophobicity and surface charge. The functionalized GO was employed as a carrier for the immobilization of lipase from Thermomyces lanuginosus (lipase TL). The storage stability, the temperature and pH sensitivity of the immobilized biocatalysts and the free lipase TL were studied. Lipase immobilized on functionalized GO demonstrates superior enzymatic loading and relative activity compared to GO, with a maximum increase of 70% and 47%, respectively. The relative activity of lipase immobilized on functionalized GO was 19.21 times more than that of commercial immobilized lipase TLIM. Through molecular dynamics simulations we concluded that the increase in the relative activity of lipase TL is mainly related to its adsorption orientation and slight conformational change. Thus, our research offered a green and effective strategy to regulate the heterogeneity of nanomaterials for a suitable microenvironment to enhance the performance of immobilized lipase.
研究领域:
生物化学
·
材料科学
·
酶工程
✓ 已验证
DOI
高影响因子
JOURNAL OF POROUS MATERIALS
·
2019
·Vol. 27
·期 2
·549-554
·
IF 1.947
Chen Meng,Wu Haoming,Li Zhentao,Wu Keke,Jiao Yanpeng,Zhou Changren
Chitin/graphene oxide (Ch/GO) composite aerogel beads were fabricated by a simple and environmentally friendly method for lipase immobilization. The morphology and specific surface area of these beads were investigated by scanning electron microscopy (SEM) and Brunauer–Emmett–Teller (BET), respectively. The results revealed that the prepared beads showed three-dimensional porous structure, and GO was successfully bound to chitin with enhanced surface area and mechanical strength. Candida rugose lipase (CRL) was immobilized on Ch/GO aerogel beads under optimum conditions by adsorption and subsequent cross-linking with glutaraldehyde which is added in the presence of the unbound proteins by conjugation. The maximum immobilization capacity of Ch, Ch/GO-5, Ch/GO-10 were 129.9, 141.1, 151.9 mg/g support . The immobilized lipases exhibited good temperature and thermal stability in comparison to free CRL. After recycling 5 times, the immobilized lipases remained more than 90% of their initial activity and the activity of Ch/GO-10 could remain 84.2% of free CRL. With these desired characteristics, the design of Ch/GO composite aerogel beads for lipase immobilization may have potential applications in various lipase-based industrial processes.
研究领域:
生物化学
·
材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2017
·Vol. 17
·期 1
·370-376
·
IF 1.483
Xia Guo-Hua,Liu Wei,Jiang Xiao-Ping,Wang Xiang-Yu,Zhang Ye-Wang,Guo Jianyong
Magnetic Fe3O4 nanoparticles were prepared through hydrothermal method and coated with silica on the surface to obtain Fe3O4@SiO2 core–shell nanoparticles. After modification with different functional groups including aldehyde,
amine and diimide, the nanoparticles were used as carrier for covalent immobilization of lipase. The nanoparticles with aldehyde groups showed highest immobilization yield (52.8%) and efficiency (86.5%). And the immobilization conditions including pH, temperature and the concentration of enzyme
were optimized. After immobilization, the K
m of lipase was altered from 2.3 to 3.2 mM. The thermal stability and pH stability were enhanced by immobilization at the investigated conditions: pH 5.0–8.0 and temperature 30–70 °C. After 10 batches conversion of
4-Nitrophenyl palmitate into p-Nitrophenol, the immobilized lipase retained over 75% of the original activity. Compared with the commercial lipase Novozym435, the immobilized lipase showed better stability and higher catalytic efficiency. These results demonstrate that the immobilized lipase
on the modified Fe3O4@SiO2 magnetic nanoparticles has enhanced stability and reusability, which make lipase of potential interest in a number of industrial applications.
研究领域:
生物化学
·
纳米材料科学
·
酶工程
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF BIOTECHNOLOGY
·
2015
·
IF 2.871
Wei Li,Huan Wu,Benguo Liu,Xuedan Hou,Duanji Wan,Wenyong Lou,Jian Zhao
Dihydromyricetin is the principle component of the Chinese herbal tea Teng-cha and a promising ingredient for functional food and nutraceuticals, but its low solubility limits its application potentials. This study explored enzymatic acylation of dihydromyricetin to improve its solubility in lipid systems. Acylation was achieved with several lipases with the synthesis of a major (>86%) product and a minor product. Isolation and purification of the products by preparative HPLC followed by LC–MS, 13 C NMR, 1 H NMR and 2 D-HSQC NMR analyses showed that the major product was a dihydromyricetin monoester with the acylation site at the 3-OH group of C ring. Quantum chemical calculations revealed that the 3-OH had the lowest antioxidant activity , and therefore acylation at this site was expected to have minimum impact on the antioxidant activity. Several factors, including solvent, acyl donor, enzyme origin, molar ratio of substrates and reaction temperature and time, exhibited significant effects on the initial rate, conversion yield and regioselectivity of the reaction. Acylation occurred only with vinyl acetate as the acyl donor, and highest conversion yields were achieved with immobilized Penicillium expansum lipase and Novozyme 435 with DMSO and acetonitrile being the best solvents. In general, the acylation results were found to be superior to previous reports on acylation of aglycone flavonoids with respects to conversion yield and regioselectivity.
研究领域:
生物化学
·
食品科学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）