Theranostics
·
2018
·
IF 8.537
Shulian Bai,Bangtian Xu,Yongcan Guo,Juhui Qiu,Wen Yu,Guoming Xie
While detection of microRNA with or without signal amplification is highly informative, nanosensors with high specificity for cell-specific RNA detection are rare. Methods: In this study, a tetrahedral DNA nanostructure (TDN) with a specific function was combined with gold nanoparticles (Au-NP) possessing fluorescence quenching effects and a large surface area to fabricate a fluorescence resonance energy transfer based nanosensor (Au-TDNN). The presence of miR-21 (target) can separate the fluorescent dye-labeled detection probe on Au-TDNNs from Au-NPs, which separates the donor and acceptor, thus inducing an intensive fluorescence signal. High specificity for discerning point mutation targets was achieved by rationally designing the nucleic acid strand displacement reaction to occur spontaneously with ΔG 0 ≈ 0 based on thermodynamic parameters; under this condition, slight thermodynamic changes caused by base mismatch exert significant effects on hybridization yield. Results: Chemically synthesized DNA of three single-base-changed analogues of target, let-7d, and miR-200b were tested. A discrimination factor (DF) of 15.4 was produced by the expected detection probe on Au-NPs for proximal single-base mismatch. As the control group, the DF produced by an ordinary detection probe on Au-NPs only reached 2.4. The feasibility of the proposed strategy was also confirmed using hepatocyte cancer cells (HepG2). Conclusion: This improved nanosensor opens a new avenue for the specific and easy detection of microRNA in live cells.
研究领域:
分子生物学
·
纳米医学
·
生物传感技术
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Liang Shi,Liangliang Lin,Lijiang Zhang,Lili Wu,Junjie Chi,Keqing Shi,Xing Dong
Tumor markers (TMs) are crucial indicators of preneoplastic and neoplastic diseases, which provide the basis for the accurate diagnosis of malignant tumors. We present a diagnostic platform utilizing magnetic photonic microspheres (mPCMs) for simultaneous detection of various sorts of TMs, specifically for reliable breast cancer identification. The multiplex detection capability of mPCMs stems from the color encoding function of the photonic crystal structure formed by self-assembled silica nanoparticles, while their magnetic responsiveness is achieved through ferric oxide nanoparticle doping. The ordered nanostructure offers a large specific surface area for biological detection, and the magnetically controlled movement enhances the capture efficiency of target biomarkers. Specifically, CA 15–3, miRNA-21, and exosomes are used as target biomarkers, reflecting different aspects of breast cancer biology. Our multivariate detection platform effectively identifies proteins, nucleic acids, and exosomes through specific binding and base complementary pairing. Thus, mPCMs provide a novel approach for the combined analysis of diverse biomarkers.
研究领域:
生物医学工程
·
肿瘤学
·
纳米技术
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生物传感器
·
分子诊断学
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Qingyou Cai,Fanfan Wang,Jingying Ge,Zhiguo Xu,Mei Li,Hui Xu,Hua Wang
Herein, we reported the G-wire-based self-quenched fluorescence probe and its application in ultrasensitive microRNA (miRNA) detection by combining with target-activated isothermal cascade amplification. The terminal-single-fluorescein (FAM)-labeled G-rich oligonucletides self-assembled into G-wire nanostructures (G-wires) with K + and Mg 2+ . Thereafter, the G-wires brought terminal-labeled FAM into close proximity, as a result, the self-quenched signal probe formed. Besides, when there was the target miRNA, target-activated isothermal cascade amplification converted miRNA into the copious trigger DNA. After hybridization between trigger DNA and the self-quenched probe, the G-wires were splited and forced the apart of proximate FAM, and then the self-quenched probe displayed an “on” mechanism. Therefore, the approach gave a limit of detection (LOM) of 0.82 aM to miRNA-21 and could be implemented within a wide linear range of 2 aM to 2 nM. This approach was able to distinguish the single-mismatched miRNA-21, which was selective and sensitive in detecting human spiked serum samples.
研究领域:
分子生物学
·
生物化学
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纳米技术
·
癌症诊断
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DOI
PubMed
高影响因子
RSC Advances
·
2012
·Vol. 2
·期 18
·7140-7145
·
IF 0
Yunlei Zhou,Mo Wang,Xiaomeng Meng,Huanshun Yin,Shiyun Ai
A novel signal amplified electrochemical microRNA (miRNA) biosensor was developed here using hemin-G-quadruplex as signal unit. A hairpin structure DNA probe (43 bases) was immobilized on the gold nanoparticles (AuNPs) modified gold electrode surface with the segment at its 3′-end complementary to miRNA-21 (22 bases) and the segment at its 5′-end complementary to capture DNA (21 bases). Upon hybridization with the target miRNA-21, the hairpin structure was unfolded, which was further hybridized with capture DNA loaded on AuNPs. The AuNP contained two kinds of DNA; one was complementary to the hairpin structure DNA probe, while the other was aptamer for hemin. The electrochemical signal of hemin self-assembled in the center of G-quadruplex structure of the aptamer was measured using chronoamperometry. The miRNA-21 target was analyzed with a detection limit of 3.96 pM. Using isolated total RNA from human gastric carcinoma BGC-823 cells, human breast adenocarcinoma MCF-7 cells, human hepatocarcinoma HepG2 cells and human colon cancer HT-29 cells, the assay detected specifically miRNA-21 and over-expression of oncogene miRNA-21 was demonstrated.
研究领域:
生物化学
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生物技术
·
分析化学
·
癌症研究
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产品关联
DOI
SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Yuanhui Ma,Xi Liu,Minjie Yang,Ling Zha,Ying Huang,Dong Sun,Ruizhuo Ouyang,Yuqin Jiang,Yuqing Miao,Baolin Liu
MicroRNA-21 (miRNA-21) is a key oncogenic biomarker for early cancer diagnosis, yet its ultrasensitive detection in serum remains challenging due to limitations of conventional methods such as low sensitivity, high background, and poor biocompatibility. This study develops an electrochemical biosensor for femtomolar miRNA-21 detection by integrating Y-shaped DNA scaffold-enhanced catalytic hairpin assembly (Y-CHA) and Bi@PtAg nanoclusters (NCs). This design achieved an ultra-low detection limit, approximately 2.8-fold lower than that of the conventional sensor. The Y-CHA strategy spatially confines hairpin probes (H1 and H2) within a Y-DNA scaffold, significantly enhancing signal amplification and hybridization efficiency. Meanwhile, the core-shell structured Bi@PtAg NCs exhibit 2.1-fold higher H₂O₂ reduction catalytic activity than monometallic nanoparticles and demonstrate low cytotoxicity (approximately 80% cell viability at 200 μg·mL⁻¹). The biosensor achieves an ultra-low detection limit of 0.05 fM with a wide linear range from 1 fM to 100 pM (R² = 0.9982). It also displays excellent specificity in distinguishing miRNA-21 from mismatched and non-complementary RNAs, good stability (87.1% signal retention after 8 days), and reliable performance in serum samples (93.0–99.7% recovery, RSD < 5%). This study develops a versatile electrochemical biosensing platform for miRNA analysis and disease biomarker detection.
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DOI
高影响因子
Science China-Chemistry
·
2020
·Vol. 63
·期 5
·731-740
·
IF 6.356
Wang Yingfei,Li Yuyi,Zhang Yue,Ren Kewei,Ju Huangxian,Liu Ying
Array based detection techniques with fluorescence signal reading is a powerful tool for multiple targets analysis. However, when applied fluorescence array for microRNA detection, time-consuming multi-steps surface signal amplification is usually required due to the low abundance of microRNA in total RNA expressions, which impairs detection efficiency and limits its application in point of care test (POCT) manner. Herein, DNA cascade reactors (DCRs) functionalized photonic crystal (PC) array was fabricated for express and sensitive detections of miRNA-21 and miRNA-155. DCRs were assembled by interval conjugation of self-quenched hairpin DNA probes to single strand DNA nanowire synthesized by rolling circle amplification, which generated cascade DNA hybridization reactions in response to target miRNA with instant fluorescence recovery signal. PC array patterns with multi-structure colors further amplified fluorescence with their respective photonic bandgaps (PBGs) matching with the emission peaks of fluorescence molecules labelled on DCRs. The as-prepared DCRs functionalized PC array demonstrated express and sensitive simultaneous detections of miRNA-21 and miRNA-155 with hundreds fM detection limits only in 15 min, and was successfully applied in fast quantifications of low abundance miRNAs from cell lysates and spiked miRNAs from human serum, which would hold great potential for disease diagnosis and therapeutic effect monitoring with a POCT manner.
研究领域:
分子生物学
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纳米技术
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生物医学工程
·
临床诊断
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Xiaofang Liu,Qun Wang,Jiawei Li,Zhan Diao,Jingzhou Hou,Danqun Huo,Changjun Hou
Simultaneous detection of multiple breast cancer-associated miRNAs significantly raises the accuracy and reliability of early diagnosis. In this work, disposable carbon fiber paper serves as the biosensing interface, linking DNA probes via click chemistry to efficiently capture targets and signals efficiently. DNA probes have multiple recognition domains that trigger a cascade reaction through the helper probes and targets, resulting in two signals output. The signals are centrally encapsulated in the pore of the MIL-88(Fe)-NH2. The signal carriers are directed by signal probes to the recognition domains that correspond to the DNA probes. The biosensor is selective and stable, and it can quantify miRNA-21 and miRNA-155 simultaneously with detection limits of 0.64 and 0.54 fmol/L, respectively. Furthermore, it demonstrates satisfactory performance in tests conducted with normal human serum and cell lysate. Overall, this method makes a satisfactory exploration to realize an inexpensive and sensitive biosensor for multiple biomarkers.
研究领域:
生物技术
·
生物传感器
·
癌症研究
·
分子生物学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2016
·
IF 4.712
Kai Shi,Baoting Dou,Jianmei Yang,Ruo Yuan,Yun Xiang
The monitoring of microRNA (miRNA) expression levels is of great importance in cancer diagnosis. In the present work, based on two cascaded toehold-mediated strand displacement reactions (TSDRs), we have developed a label- and enzyme-free target recycling signal amplification approach for sensitive electronic detection of miRNA-21 from human breast cancer cells. The junction probes containing the locked G-quadruplex forming sequences are self-assembled on the senor surface. The presence of the target miRNA-21 initiates the first TSDR and results in the disassembly of the junction probes and the release of the active G-quadruplex forming sequences. Subsequently, the DNA fuel strand triggers the second TSDR and leads to cyclic reuse of the target miRNA-21. The cascaded TSDRs thus generate many active G-quadruplex forming sequences on the sensor surface, which associate with hemin to produce significantly amplified current response for sensitive detection of miRNA-21 at 1.15 fM. The sensor is also selective and can be employed to monitor miRNA-21 from human breast cancer cells.
研究领域:
生物化学
·
分子生物学
·
癌症诊断
·
纳米技术
·
电化学生物传感器
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 8
·1-9
·
IF 5.7
Xia Qing,Zheng Jintao,Bu Jianlan,Li Rui,Li Xinxin,Fan Shuting,Ling Kai,Jiang Hongyan
The development and performance of a DNA probe adsorbing Mn 2+ -modified black phosphorus (BP@Mn 2+ /DNA) hybrid nanosensor is reported that enables rapid detection of cancer-derived exosomal microRNAs (miRNAs) and exosomes. This two-dimensional (2D) nanosensor can spontaneously penetrate the lipid bilayer of exosome membranes owing to its ultra-thin geometry. Subsequently, the adsorbed probe specifically hybridizes with the target miRNA and then dissociates from the nanosensor surface, generating fluorescent signals. Therefore, the BP@Mn 2+ /DNA nanosensor can differentiate between colorectal cancer (CRC) cell-derived exosomes and those derived from intestinal epithelial cells through sensing of exosomal miRNAs. Furthermore, when the epithelial cell adhesion molecule (EpCAM) aptamer is adsorbed onto BP@Mn 2+ instead of the miRNA probe, the nanosensor is able to distinguish exosomes derived from the plasma of CRC patients from those of healthy controls by the recognition ability of the EpCAM aptamer. By utilizing this nanosensor, we were able to effectively differentiate cancer-derived exosomes through the direct detection of miRNA-21 within the exosomes, as well as the identification of specific exosomal membrane proteins. This nanosensor design paves the way for the development of rapid and efficient cancer-derived exosomal miRNA and exosome biosensing nanoplatforms. Graphical abstract
研究领域:
纳米技术
·
癌症生物学
·
分子诊断
·
生物传感器
·
细胞外囊泡
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2020
·
IF 3.807
Xialing Hou,Zhiguang Suo,Ziheng Hu,Xinying Zhang,Yingying Chen,Lingyan Feng
A label-free microRNAs (miRNAs) electrochemiluminescence sensor was developed based on the synergistically enhanced ECL signal using novel ECL nanomaterial assembled by tri-luminophores perylenetetracarboxylic acid (PTCA) graphite-like carbon nitride nanosheet (g-C 3 N 4 NS) and graphene carbon quantum dots (GQDs). The ECL intensity of our proposed nanocomposites increased significantly, by taking advantages of the synergistic promotion ECL mechanism of PTCA g-C 3 N 4 and GQDs. Meanwhile, a three-way junction (TWJ) structure was introduced into the sensor design, whose conformation can be specifically induced in the presence of target miRNA. Thus, the formation of TWJ structure can hinder the contact between the co-reactant Na 2 S 2 O 8 in the solution phase and the luminescent substrate, which produce a quenched ECL signal. Our proposed ECL biosensor provided a highly sensitive detection of miRNA-21 with a detection limit of 96 aM ranging from 100 aM to 1 nM. Furthermore, this ECL sensing system was able to successfully distinguish the target RNA from its homologous family and presented a promising response in human real samples. With excellent properties, the proposed ECL biosensor provided an efficient and ultrasensitive method for miRNAs detection.
研究领域:
分析化学
·
生物化学
·
分子生物学
·
纳米技术
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Jiawei Shen,Tingting Li,Min Wang,Bo Yao
MicroRNAs (miRNAs) are a category of short non-coding RNA molecules which play essential roles in regulating gene expression. Correspondingly, the aberrant expression of miRNAs is commonly related to the development of various diseases including cancers and cardiovascular diseases. Hence, miRNA is considered as an ideal biomarker for disease diagnosis and its quantification is highly demanded. Herein, an isothermal and enzyme-free miRNA assay based on catalytic hairpin assembly (CHA) amplification coupled with inductively coupled plasma mass spectrometry (ICP-MS) detection is proposed. Two hairpin probes are employed in CHA reaction with rare earth element tag on H1 for ICP-MS detection and biotin on H2 for immobilization on magnetic beads. In the presence of target miRNA, the two independent probes open up their stem-loop structure and hybridize. The target miRNA will be released after the hybridization to trigger another CHA reaction. Ultimately, one target miRNA molecule promotes a number of CHA cycles and produces several copies of hybridized complex leading to the enhanced ICP-MS signal. Based on this approach, miRNA-141 was successfully analyzed with high sensitivity and specificity. The limit of detection (LOD) was 88 fmol with a linear range 0.1–2 pmol. Compared with fluorescence mediated CHA method and ICP-MS mediated basic sandwich method, the current strategy displayed the lowest LOD. The developed method was also applied to analyze miRNA-141 and miRNA-21 simultaneously to demonstrate its multiple detecting capability and practical applicability. Furthermore, this miRNA assay has the potential to become a high-throughput method by employing more distinct rare earth elements.
研究领域:
分子生物学
·
分析化学
·
生物医学工程
·
癌症研究
·
心血管疾病
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Weixiang Huang,Chunli Yang,Jiaxi Gao,Jingjing Ye,Ruo Yuan,Wenju Xu
Exploring the cooperative amplification of peroxidase-like metal nanocomposites and cycled hairpin assembly is intriguing for sensitive bioanalysis. Herein, we report the first design of a unique electrochemical biosensor based on mimicking Au@FeCo nanozymes and bicycled hairpin assembly (BHA) for synergistic signal amplification. By loading the enzyme-like FeCo alloy in Au nanoparticles (AuNPs), the as-synthesized Au@FeCo hybrids display great improvement of electronic conductivity and active surface area and excellent mimic catalase activity to H2O2 decomposition into •OH radicals. The immobilization of Au@FeCo in an electrode sensing interface is stabilized via the resulting electrodeposition in HAuCl4 while efficiently accelerating the electron transfer of electroactive ferrocene (Fc). Upon the immobilization of a helping hairpin (HH) via Au–S bonds, a specific DNA trigger (T*) is introduced to activate BHA operation through competitive strand displacement reactions among recognizing hairpin (RH), signaling hairpin (SH), and HH. T* and RH are rationally released to catalyze two cycles, in which the transient depletion of dsDNA intermediates rapidly drives the progressive hairpin assemblies to output more products SH·HH. Thus, the efficient amplification of Au@FeCo mimic catalase activity combined with BHA leads to a significantly increased current signal of Fc dependent on miRNA-21 analogous to T*, thereby directing the creation of a highly sensitive electrochemical biosensor having applicable potential in actual samples.
研究领域:
电化学
·
纳米技术
·
生物化学
·
生物传感器
·
催化
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）