查找使用阿拉丁产品的科研文献

An injectable thermosensitive PLGA–PEG–PLGA hydrogel integrated with coordination-driven self-assembled MTX–Mn nanoparticles for enhanced melanoma therapy via mitochondrial dysfunction

Xiaolong Xu,Yuze Zhou,Junhao Chen,Lujing Fei,Min Qi

The treatment of malignant melanoma, an aggressive skin cancer with high recurrence and metastatic potential, often presents challenges due to systemic toxicity and multidrug resistance in current therapies. In this study, we develop a localized therapeutic platform by integrating methotrexate–manganese (MTX–Mn) coordination nanoparticles into a thermosensitive PLGA–PEG–PLGA (PPP) hydrogel to achieve sustained and site-specific drug delivery for melanoma treatment. MTX–Mn nanoparticles were synthesized through coordination-driven self-assembly and comprehensively characterized by FTIR, 1H NMR, TEM, EDS, and zeta potential analyses, confirming successful coordination and uniform nanoscale features. In vitro experiments demonstrated that MTX–Mn significantly inhibited melanoma cell proliferation and migration. Flow cytometry further revealed that MTX–Mn induced pronounced G0/G1 cell-cycle arrest and effectively promoted apoptosis, which was confirmed by TUNEL staining. Mechanistic investigations indicated that MTX–Mn triggered severe mitochondrial dysfunction, including ultrastructural damage, elevated mitochondrial ROS generation, and collapse of mitochondrial membrane potential (JC-1), suggesting a mitochondria-associated antitumor mechanism. In vivo, peritumoral administration of PPP/MTX–Mn markedly suppressed tumor growth in a melanoma-bearing mouse model, reduced the tumor burden, and significantly decreased Ki-67 expression. Histological evaluation of major organs by H&E staining revealed no obvious pathological abnormalities, supporting good in vivo biosafety. Overall, this MTX–Mn-loaded thermosensitive hydrogel provides a promising strategy for effective and safe localized melanoma therapy.

Oxidation-responsive PEG-poly(α-lipoic acid) nanoparticles for coenzyme Q10 delivery attenuate hepatic ischemia-reperfusion injury via ROS scavenging and ferroptosis inhibition

Yu Guan,Jinyu Liu,Jing Yang,Sufang Chen,Weifeng Yao,Shengyuan Gong,Chunsheng Xiao,Mingqiang Li,Gangjian Luo,Ziqing Hei

Hepatic ischemia-reperfusion injury (IRI) is characterized by an acute surge of reactive oxygen species (ROS) upon reperfusion, leading to oxidative damage and cell death. Ferroptosis, a form of iron-dependent lipid peroxidation-driven cell death, has recently been implicated in hepatic IRI, compounding the injury. Here, we present an oxidation-responsive nanoparticle system designed to mitigate liver IRI by scavenging ROS and inhibiting ferroptosis. We synthesized a PEGylated poly(α-lipoic acid) (PEG-PαLA) copolymer that self-assembles into nanoparticles encapsulating the lipophilic antioxidant coenzyme Q10 (CoQ10). The PEG-PαLA/CoQ10 nanoparticles have an average diameter of ~100 nm and release CoQ10 preferentially under oxidative conditions. In vitro, the nanoparticles efficiently neutralized free radicals and protected hepatocytes from oxidative injury. In a mouse model of partial hepatic IRI, treatment with PEG-PαLA/CoQ10 nanoparticles significantly reduced liver injury markers, preserved liver histology, and abrogated lipid peroxidation, indicating suppression of ferroptosis. Our results demonstrated that PEG-PαLA/CoQ10 nanoparticle is a novel antioxidant nanomedicine that synergistically attenuates ROS-mediated damage and ferroptotic cell death in hepatic IRI. These findings highlight a promising strategy for protecting organs from ischemia-reperfusion damage by targeting oxidative stress and ferroptosis with responsive biomaterial carriers.

Multilayered PCL@PEG/Al₂O₃-ADP phase change composite film with foldability, leakage-proof and flame retardancy for lithium-ion battery thermal management

Tianren Zhao,Yuanqing Cao,Jiarui Dong,Hong Zhang,Chengzhi Wang,Zengguo Feng,Haibo Jin,Aiying Zhang

The quest for ever-growing energy density of lithium-ion batteries dictates urgent concerns about their thermal safety. Phase change materials (PCMs) are considered promising for battery thermal management, yet their extensive application is restricted by inherent limitations such as leakage risk, low thermal conductivity, and flammability. Here, we fabricate a multilayered PCL@PEG/Al₂O₃-ADP (CEAD) composite film through strategy of coaxial electrospinning and dip-coating. Benefiting from the effective PEG encapsulation, thermally conductive Al₂O₃ fillers, and an ADP coating for flame retardancy, the composite film demonstrates excellent shape stability with no leakage even after 12 h of continuous exposure at 50 °C, high phase change enthalpy, improved thermal conductivity, and self-extinguishing behavior. Its notable foldability also ensures facile installation. When wrapped around a lithium-ion battery, the film maintains the battery temperature below 45 °C under a 5C discharge rate, which is 13 °C lower compared to the bare one. Thus, the composite film provides simultaneous efficient thermal management and flame retardancy, meeting critical safety requirements for high energy density lithium-ion batteries. This work offers a viable pathway for developing multifunctional thermal management materials suitable for advanced battery systems and other electronics.

Enhanced cycling performance for all-solid-state lithium ion battery with LiFePO4 composite cathode encapsulated by poly (ethylene glycol) (PEG) based polymer electrolyte

Huihui Zeng,Xiaoxiao Ji,Fangchang Tsai,Qunchao Zhang,Tao Jiang,Robert K.Y. Li,Hengchong Shi,Shifang Luan,Dean Shi

The poor contact between the cathode and the solid polymer electrolyte (SPE) is one of the primary causes for the low specific capacities of lithium ion batteries . In this work, a composite cathode material containing LiFePO 4 nanoparticles encapsulated by PEG-based polymer electrolyte (coated LiFePO 4 ) are prepared in order to increase the interfacial contact area between SPE and cathode materials. The LiFePO 4 /SPE/Li half-cell battery with coated LiFePO 4 (C-LFP) and uncoated LiFePO 4 (U-LFP) are assembled and their electrochemical performances are studied. The optimal LiFePO 4 /SPE weight ratio in the composites cathode is 60/15. When the cathode thickness is 100 μm, the specific capacity of battery with C-LFP can reach up to 138 mAhg −1 , which is 2.5 times higher than that with U-LFP under 1C charge-and-discharge rate at 60 °C. Moreover, the capacity retention of C-LFP is 91% after 300 cycles, while that of U-LFP is only 18%. Large cracks emerge in the cathode with U-LFP, while no damage can be found in that with C-LFP after 300 cycles at 1C. This should be ascribed to the existence of polymer buffer layers among LiFePO 4 nanoparticles to alleviate the stress induced by their volume changes during the charge-and-discharge cycling process. This enhancement effect increases along with the increase of cathode thickness. When the cathode thickness increases up to 200 μm, the specific capacity of battery with C-LFP cathode is almost 4 times higher than that with U-LFP.

The properties of mesoporous silica nanoparticles functionalized with different PEG-chain length via the disulfide bond linker and drug release in glutathione medium

Zhifei Xie,Huameng Gong,Mingxing Liu,Hongda Zhu,Honghao Sun

In this paper, a novel drug-loaded material (MSNs-SS-PEG) was obtained by grafting the thiol-linked methoxy polyethylene glycol (MeOPEG-SH) onto the thiol-functionalized mesoporous silica nanoparticles (MSNs-SH) via the disulfide bond linker. In our designed experiment, three different chain lengths of PEG (PEG1000, PEG5000, and PEG1000-PEG5000) were used. The silica materials were characterized by Fourier transform infrared spectroscopy (FT-IR), dynamic light scattering, field emission scanning electron microscopy, transmission electron microscopy, nitrogen adsorption–desorption measurements, and X-ray diffraction. The morphology of the MSNs-SS-PEG was spherical with an average diameter of about 150 nm. Due to the covalent modification of hydrophilic MeOPEG, the MSNs-SS-PEG was coated by a thin polymer shell, showing stable and inerratic MCM-41 type mesoporous structure as well as high specific surface areas and large pore volumes. Moreover, the releases of doxorubicin hydrochloride (DOX) from these materials at 10 mM of glutathione were investigated. The PEG functionalization could effectively cap drugs in the mesoporous channels. The release of DOX from the MSNs-SS-PEGn revealed redox-responsive characteristic. The obtained results showed that the MSNs-SS-PEG might be promising drug delivery carrier materials, which could play an important role in the development of drug delivery.

Investigations on porous PLA composite scaffolds with amphiphilic block PLA-b-PEG to enhance the carrying property for hydrophilic drugs of excess dose

Tian Zhong,Yang Jiao,Lingling Guo,Jiamin Ding,Zhuping Nie,Lianjiang Tan,Ran Huang

To address concern of the dispersion property of hydrophilic drugs of excess dose loaded in a hydrophobic poly(lactic acid) (PLA) matrix, this work developed a PLA and PLA -b -polyethylene glycol (PEG) composite scaffold ( ) and studied its carrier properties for aspirin as a model hydrophilic drug. The porous functional scaffolds were prepared from PLA and PLA -b -PEG solutions with the dose of 5, 10, and 15 wt % aspirin preloaded. The products and control samples of pure PLA with the same loading amount for comparison were characterized by scanning electron microscopy and X-ray diffraction to examine the miscibility and porous structure. Rapid degradations in a strongly basic solution were performed to determine the actual loading amount and the encapsulation ratio. The in vitro release in phosphate buffer saline (PBS) at 37.5 °C indicated that the addition of amphiphilic block polymer may efficiently enhance the dispersion property and stabilize the release of hydrophilic drugs, especially with a high loading dose. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017 , 134 , 44489.

Fabrication of an ultrathin PEG-modified PEDOT:PSS HTL for high-efficiency Sn–Pb perovskite solar cells by an eco-friendly solvent etching technique

Pengju Guo,Jun Dong,Cunyun Xu,Yanqing Yao,Jiayu You,Hongyu Bian,Wenqi Zeng,Guangdong Zhou,Xiaofeng He,Meng Wang,Xianju Zhou,Min Wang,Qunliang Song

Poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) is widely used as a hole transport layer (HTL) in inverted perovskite solar cells (PSCs) because of its excellent hole transport capability and simple preparation process. According to theoretical studies, a thinner PEDOT:PSS HTL allows more incident light to enter the perovskite layer. However, the PEDOT:PSS HTL prepared by the traditional deposition process has some limitations, such as the thick HTL, abundant defects at the PEDOT:PSS/perovskite interface, etc. Is there a preparation process that can achieve the thinning and modification of the PEDOT:PSS HTL in one-step process at the same time? In this work, inspired by the semiconductor etching process, an eco-friendly solvent etching technique is applied during the spin-coating process to fabricate an ultrathin PEDOT:PSS HTL and simultaneously introduce a green passivator (polyethylene glycol, PEG) as the bridge molecule on the HTL upper surface. In this process, the green PEG aqueous etchant can etch the excess bulk PEDOT:PSS away to form an ultrathin PEDOT:PSS HTL with PEG molecules and rich PEDOT domains remained on its surface (PEG-PEDOT:PSS HTL). This well-designed HTL is successfully thinned to enhance its light transmission and can be better matched with the Sn–Pb perovskite layer. As a result, the power conversion efficiency of Sn–Pb PSCs using the PEG-PEDOT:PSS HTL is improved from 20.52% of the reference to 21.58%, and the stability of Sn–Pb PSCs is dramatically enhanced as well.

PEG-modified halloysite as a hydrophilic interaction and cation exchange mixed-mode sorbent for solid-phase extraction of biogenic amines in fish samples

Wang Tingting,Chen Yihui,Hou Chunyan,Qiao Xiaoqiang

A novel type of PEG-modified halloysite was prepared and used as a hydrophilic interaction and cation exchange mixed-mode sorbent for solid-phase extraction of biogenic amines in fish samples. The eluates were analyzed by high-performance liquid chromatography-ultraviolet detection after the derivatization with benzoyl chloride. The developed sorbent was characterized by scanning electron microscopy, infrared spectroscopy, X-ray diffraction, zeta potential analyzer, and thermo-gravimetric analysis. After the optimization of various parameters influencing the extraction efficiency, the PEG-modified halloysite-based SPE method was evaluated. The adsorption capacities of putrescine, spermine, phenethylamine, and histamine were as high as 9.3, 8.5, 5.7, and 5.6 mg g −1 , respectively. Satisfactory reproducibility of sorbent preparation was obtained with within-batch and batch-to-batch relative standard deviations (RSDs) lower than 3.9% and 8.6%, respectively. The biogenic amine spiking recoveries in fish samples ranged from 84.3 to 105.5% with good RSDs lower than 7.8%. Intra-day and inter-day precision, expressed as RSDs, were better than 8.8%. The limits of detection of histamine, putrescine, phenethylamine, and spermine were 9.4, 1.9, 0.5, and 0.9 μg L −1 , respectively. This work provides a new hydrophilic interaction and cation exchange mixed-mode sorbent and is successfully applied to the extraction of trace biogenic amines from fish samples. Graphical Abstract

FRET-based analysis on the structural stability of polymeric micelles: Another key attribute beyond PEG coverage and particle size affecting the blood clearance

Chen Guo,Haoyang Yuan,Ying Yu,Zhencheng Gao,Yu Zhang,Tian Yin,Haibing He,Jingxin Gou,Xing Tang

Various attributes of micelles , such as PEG density and particle size, are considered to be related to blood clearance. The structural stability of micelles is another key attribute that will affect the in vivo fate. This study employed fluorescence resonance energy transfer (FRET) analysis to guide the preparation of polymeric micelles with different structural stability. Micelles prepared using copolymers with longer hydrophobic blocks showed higher structural stability; emulsification was a better method than nanoprecipitation to prepare stable micelles. The fast chain exchange kinetics and the high-water content of micellar cores explained the low structural stability of those micelles. Moreover, this study highlighted the importance of structural stability that affected blood clearance in concert with PEG length and particle size. One-third of the small and stable micelles were detected in the blood 24 h after injection. While unstable micelles would be cleared from the circulation within 4 h. Notably, there would be a threshold of structural stability. Micelles with structural stability below this threshold were quickly cleared even if they possessed a longer PEG length and a smaller size. In contrast, higher structural stability allowed polymeric micelles to maintain higher integrity in vivo and enhance tumor accumulation and anti-tumor efficacy. In conclusion, this study systematically analyzed the importance of the structural stability of micelles on the in vivo fate.

The detection of brucellosis antibody in whole serum based on the low-fouling electrochemical immunosensor fabricated with magnetic Fe3O4@Au@PEG@HA nanoparticles

Shuli Lv,Jinliang Sheng,Shiyi Zhao,Mingchao Liu,Lihua Chen

It is a novel competitive challenge for electrochemical biosensor to directly, rapidly and ultrasensitively detect the disease markers in the whole serum due to biofouling caused by the complexity of actual samples. In this paper, poly (ethylene glycol) (PEG) and hyaluronic acid (HA) were utilized to modify Fe 3 O 4 @Au nanoparticles (NPs). Based on the successfully preparation and characterization of Fe 3 O 4 @ [email protected] @HA NPs with TEM , SEM, XRD, FTIR and EDS , respectively, a novel immunosensor of brucellosis with high selectivity , sensitivity and almost perfect protein-resistant properties in various external environments, especially, in complex biological systems was fabricated. More importantly, this immunosensor is capable of assaying brucellosis antibody in 100% serum without suffering from any significant biological interference. In addition, a wide linear response range from 10 –15 g mL −1 to 10 –11 g mL −1 towards antibody in 100% serum and a low limit of detection (LOD) of 0.36 fg mL −1 (3σ, n = 13) are demonstrated, which indicates that this immunosensor has a promising potential in clinical diagnosis.

Tailor-made PEG-DA-CuS nanoparticles enriched in tumor with the aid of retro Diels–Alder reaction triggered by their intrinsic photothermal property

Jie Sheng,Beibei Ma,Qian Yang,Chao Zhang,Zhongying Jiang,Entomack Borrathybay

Green and Efficient PEG-Based Ultrasonic-Assisted Extraction of Polysaccharides from Tree Peony Pods and the Evaluation of Their Antioxidant Activity In Vitro

Xiaonan Zhang,Qingfeng Ban,Xibo Wang,Zhongjiang Wang

We adopted and developed an ultrasonic-assisted extraction method to obtain polysaccharides from tree peony pods using polyethylene glycol (PEG) as the solvent. The technological parameters have been designed as a single factor to enhance the tree peony pod polysaccharide extraction yield. Specific conditions (ultrasound irradiation power, 250 W; ultrasound irradiation time, 30 min; reaction temperature 50°C; liquid-solid ratio, 25 mL/g; and concentration of PEG, 0.2 g/mL) generated an experimental yield of 14.14% 0.44%. Subsequently, the monosaccharide composition of the tree peony pod polysaccharides was determined by HPLC using a 1-phenyl-3-methyl-5-pyrazolone precolumn derivatization method. The results indicated that tree peony pod polysaccharides contained mannoses, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose, and fucose with a molar ratio of 1.44 : 2.87 : 0.32 : 18.99 : 3.99 : 10.21 : 0.96 : 1.85 : 0.21. The tree peony pod polysaccharides obtained are mainly galacturonic acid and galactose, which are acidic polysaccharides. Finally, the antioxidant activities (DPPH and FRAP) of the tree peony pod polysaccharides were assessed, and the compounds exhibited moderate antioxidant activities.