PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2025
·
IF 4
Xin-Yv Li,Yu-Jie Liu,Xian-Hua Meng,Chu Gong,Jun-Li Yang,Yin Qiang,Jun Wang,Xiu-Dan Hou
Anthracnose is a highly contagious and destructive plant disease caused by Colletotrichum gloeosporioides . The objective of this study was to investigate the antifungal activity and mechanism of ginsenoside Rk1 ( GRk1 ) against C. gloeosporioides. In vitro experiments demonstrated that GRk1 exhibited substantial antifungal activity, with EC 50 values of 64.58 mg L −1 , surpassing the plant-derived pesticide d -limonene . Additionally, GRk1 could effectively suppress the dissemination of anthracnose spots in postharvest fruit. By observing the microstructure of C. gloeosporioides , it was observed that the mycelium structure underwent notable changes, and autophagosomes engulfed the organelles in the experimental groups. Fluorescent staining revealed specific signals in mycelial autophagosomes, and Western Blot analysis confirmed the upregulation of the autophagy-related protein Atg8. These findings indicate that GRk1 may activate the autophagy process by promoting Atg8 expression. Proteomic analysis revealed that the expression of Sec20 and Mpv17 proteins was significantly downregulated, whose dysregulated expression is associated with the crosstalk between SNAREs and peroxisomes during vesicle trafficking and metabolic processes. Assessment of catalase (CAT) activity, hydrogen peroxide (H₂O₂) content, and superoxide anion (O₂ − ) levels further demonstrated that GRk1 could disrupt intracellular reactive oxygen species (ROS) homeostasis in mycelia. Moreover, the introduction of the ROS scavenger N -acetylcysteine (NAC) markedly inhibited autophagy, indicating that GRk1 promotes autophagy by inducing oxidative stress. Collectively, GRk1 inhibits pathogens via the ROS-autophagy axis. These findings suggested that GRk1 holds potential as a promising alternative to fungicides for the biological control of anthracnose.
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CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Jinlong Li,Zihuan Zhao,Qihang Wei,Hongman Hou,Gongliang Zhang,Jingran Bi
This study integrates UiO-66-COOH metal–organic frameworks (MOFs) with pectin (Pec) to precisely release potassium cinnamate (PC) to develop a pectinase-responsive active fruit-preservation system (UiO-66-COOH@PC/Pec). Carboxylated UiO-66 with nanoporosity was fabricated to achieve a high loading amount of PC (235 mg·g −1 ) via the synergistic effects of hydrogen bonding, as shown by the loading kinetics and reduced density gradient analyses. A gatekeeper was established by grafting Pec onto the surface of the MOF (UiO-66-COOH@PC/Pec), thereby “locking” PC to prevent leakage. Moreover, an enzyme-responsive controlled-release behavior was developed under pectinase stimulation, achieving up to 95 % PC release within 9 h through Fickian diffusion and only 25.3 % in enzyme-free conditions by non-Fickian diffusion and matrix erosion. With significant long-term inhibiting activity against both bacteria and fungi, UiO-66-COOH@PC/Pec successfully extended the shelf life of strawberries from 2 to 5 days at 25 °C. Moreover, UiO-66-COOH@PC/Pec exhibited good water solubility and negligible toxicity, indicating that it can be easily removed from fruit surfaces and offers high consumer acceptance. These results provide a novel MOF-based controlled-release strategy for smart food-packaging applications.
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Fermentation-Basel
·
2026
·Vol. 12
·期 2
·102
·
IF 4.1
Xun Fu,Qingyu Nie,Xiang Li,Penghao Tan,Tingting Feng,Chunmei Xiong,Wenling Zhang,Yan Zhang,Sujin Li,Lixin Zhang
In recent years, there has been substantial global progress in screening yeasts for fermenting various specialty fruits, especially non-Saccharomyces species known for their contributions to aroma enhancement. This study focused on mature fruits and soil samples collected from orchards located in the main production region ofC. reticulatacv. ‘Dahongpao’ (CRCD) in China, with the objective of isolating specialized non-Saccharomycesyeasts suitable for producing CRCD fruit wine. After enrichment cultivation, seven characteristic yeast strains were isolated and purified. These isolates were identified asCandida parapsilosis,Meyerozyma caribbica,Candida quercitrusa, andMeyerozyma guilliermondiithrough a combination of microscopic morphology and molecular biology methods, which also includedPichia fermentans,Pichia kudriavzevii, andPichia kluyveri. The strains’ fermentation potential, ethanol production rates, and tolerance levels were assessed, leading to the selection ofCandida parapsilosis,Candida quercitrusa,Pichia fermentans,Pichia kudriavzevii, andPichia kluyverifor further fermentation experiments. The commercial yeast La-Ma was used as a control. Analysis of volatile organic compounds (VOCs) in the fruit wine samples was performed using Gas Chromatography–Ion Mobility Spectrometry (GC-IMS). A total of 42 different VOCs were identified, with esters being the most prevalent. The fingerprint profiles demonstrated notable differences between the fruit wine samples fermented with selected yeasts and those fermented with commercial yeasts. Principal component analysis (PCA) indicated thatPichia kluyveridisplayed the most significant divergence from both commercial and other selected yeasts. The samples contained notable VOCs such as 2-methyl-1-butanol, pentanal, 3-methyl-2-butenal, propyl acetate, butyl acetate, isobutyl acetate, isopentyl acetate, and 3-methyl-2-butenyl acetate, while the methanol production was observed to be lower compared to other samples. Consequently, this strain has the potential to produce distinctive fruit wine.
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Lin Zhang,Ruifen Zhang,Mingwei Zhang,Zhenlin Xu,Fei Huang,Xuchao Jia,Lihong Dong,Zhiming Wang,Mei Deng
A debittering method by multi-enzyme coupled hydrolysis for Shatianyu ( Citrus grandis L. Osbeck) whole fruit powder (SWFP) was established. Shatianyu whole fruit homogenate (SWFH) was hydrolyzed by pectinase and cellulase (1:1, w /w, pH 4.5, 50 °C for 2 h). The mixture was then hydrolyzed by naringinase under the optimal conditions: naringinase addition amount of 0.38% ( w /w FW), hydrolysis time for 3.0 h, and hydrolysis temperature at 50 °C. Subsequently, the hydrolyzed products were freeze dried to obtain PCNE-SWFP. Compared with SWFP, the major bitter flavonoids including naringin, melitidin and cigranoside B in PCNE-SWFP were degraded by 40.1%, 37.9% and 49.7%, respectively, and bitterness intensity was reduced by 68.7%. Notably, PCNE-SWFP still showed high total flavonoid content (18.2 mg CE/g DW), also exhibited an increased soluble dietary fiber content, antioxidant activity, as well as water adsorption, water swelling and oil adsorption capacity compared with SWFP.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2026
·
IF 5.4
Zhenzhen Wu,Chunyu Wang,Yingying He,Hongfu Bi,Feng Chen,Yong Ye,Gang Chen
To address petroleum-based packaging pollution and postharvest food waste, developing biodegradable composite films with tailored physicochemical properties is highly desirable. Herein, a sustainable composite film comprising soy protein isolate (SPI), cellulose nanocrystals (CNCs), and lignin–zinc oxide nanoparticles (LZNPs) was fabricated via Ca²⁺-mediated ionic cross-linking and non-covalent interactions. The synergistic hydrogen bonding and ionic cross-linking formed a robust three-dimensional polymer network, significantly enhancing the tensile strength of the film and establishing excellent barrier properties against water vapor, oxygen, and carbon dioxide. Specifically, the LZNPs functionalized with phenolic hydroxyl groups and Zn²⁺ ions endowed the film with strong antioxidant activity (free-radical scavenging rates >70%) and potent antibacterial activity (nearly complete inhibition of the growth of both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus ). Consequently, this high-performance composite film effectively preserved bananas, plums, and cherry tomatoes, extending their shelf life for over 10 days. Moreover, the film exhibited excellent biosafety in seed germination assays and achieved complete soil biodegradation within 30 days. This work provides a promising strategy for developing biodegradable multifunctional packaging materials and demonstrates significant potential for advancing sustainable fruit preservation. Download: Download high-res image (240KB) Download: Download full-size image
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Yueru Ma,Yuqing Yang,Zhiwei Liu,Hongyu Shi,Zixin Jin,Ziwuzhen Wang,Wei Sheng
High-sensitive fluorescence biomimetic sensor for CBZ is developed. • Ni-MOF is combined with specific adsorption capacity of MIP. • Ni-MOF@MIP probe shows high specificity and sensing sensitivity for CBZ. • Adsorption capacity of Ni-MOF@MIP for CBZ has good stability. A novel fluorescence biomimetic sensor with high selectivity and sensitivity for the detection of carbendazim (CBZ) in fruits, vegetables and grains is established. This sensor is assembled from nickel-based metal-organic framework (Ni-MOF) and molecularly imprinted polymer (MIP). Here, Ni-MOF serves as the fluorescent signal source and carrier, and MIP is coated on the surface of Ni-MOF as a biomimetic recognition element for CBZ to prepare Ni-MOF@MIP probe. The fluorescence of Ni-MOF@MIP will be quenched after adding CBZ, and the fluorescence difference can be applied to realize the quantitative detection of CBZ. The sensor shows wide linear range of 0.01 to 5.00 × 10 3 μg/L ( R 2 = 0.996), and the detection limit (LOD) is 8.4 × 10 −3 μg/L (S/N = 3). The recovery of CBZ in spiked fruits, vegetables and grains has been confirmed by high performance liquid chromatography (HPLC). The fluorescent sensor provides a promising strategy for the detection of CBZ residue in fruit, vegetable and grain samples. Download: Download high-res image (163KB) Download: Download full-size image
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Foods
·
2025
·Vol. 14
·期 13
·2266
·
IF 5.1
Teng Wei,Shengyue Zhong,Bin Huang,Kang Zha,Jing Li,Qiang Wen
Camellia chekiangoleosaHu. (C. chekiangoleosa) is a typical high-altitude oil-tea Camellia species. Due to altitude being an important factor affecting crop growth and quality, the influence of environmental conditions associated with low (60 m) and high (600 m) altitudes on the economic and quality characteristics of fruit ripening was assessed in this study. Our investigations showed that altitude has no influence on the growth pattern ofC. chekiangoleosafruit shells and seed oils, and the differences in samples between different altitudes gradually decreased with the ripening ofC. chekiangoleosa. Nevertheless, matureC. chekiangoleosafruit shells and seed oils from low and high altitudes showed some differences. Specifically, the fruit shells ofC. chekiangoleosacultivated in low-altitude areas contained more soluble sugar, protein, total polyphenols, total flavonoids, and tea saponin. Meanwhile, low-altitude cultivation elevated the abundance of α-tocopherol, β-sitosterol, β-amyrinol, flavonoids, and polyphenols in mature seed oils but decreased the oil yield. Moreover, few effects of altitude on fatty acid composition were observed in mature seed oils. Cluster and receiver operating characteristic (ROC) analysis indicated that the influence of altitude on the quality of mature seed oils was strongly associated with oil yield and α-tocopherol. Taken together, the present study suggests that when cultivatingC. chekiangoleosain low-altitude regions, more energy should be devoted to improving oil yield. The results of the fruiting process and quality trait variation inC. chekiangoleosaduring the low-altitude introduction process can provide an important theoretical basis for the introduction and cultivation of this oil-tea species.
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Food Chemistry-X
·
2026
·
IF 8.2
Mingyu Zhu,Zhiyun Peng,Guangcheng Wang
The fruit of Rosa roxburghii Tratt. (RRT) is frequently eaten in Guizhou Province, China. Thirty compounds from RRT fruit were identified by the bioaffinity-ultrafiltration method. Among them, quercetin, diosmetin, luteolin, and apigenin (IC 50 = 4.80 ± 0.12 μg/mL, 52.62 ± 0.26 μg/mL, 17.51 ± 0.27 μg/mL, 33.24 ± 0.32 μg/mL) exhibited significant α-glucosidase inhibitory activities. Based on multispectral and molecular docking analyses, these active compounds bind to the active site of α-glucosidase via at least four hydrogen bonds and van der Waals forces, thereby altering its hydrophobic environment and secondary structure. in vivo studies showed that compared to the AUC of postprandial glucose in the model (2809.33 ± 64.84 mM·min) and in the acarbose group (2525.40 ± 54. 28 mM·min), these compounds decreased postprandial blood glucose levels. Long-term administration of apigenin additionally improved lipid metabolic disorders. These results support the development of RRT fruit as a hypoglycemic functional food.
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POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2017
·
IF 3.248
Ting-Tiao Pan,Hongbin Pu,Da-Wen Sun
In this paper, fresh pear fruits and those infected by Alternaria alternata ( A. alternata ) were studied by confocal Raman microspectroscopy (CRM) to illustrate the changes in chemical compositions of cell wall. Firstly, Raman spectra of the cell wall of both fresh and infected fruits were collected with spatial resolution at micron level, and then label-free in situ imaging of chemical compositions in the cell wall were mapped. The results showed that there were significant changes in the signal intensity of cell wall, especially in the later stage of A. alternata infection. After 8 days of infection at room temperature, the signal intensities at 1086 and 871 cm −1 , which were associated with cellulose and pectin, were decreased by 58.50% and 58.67%, respectively, revealing changes in the main components of the infected cell wall infected. Meanwhile, the chemical images of the cell wall of both fresh and infected fruits were compared, indicating that A. alternata infection caused the alterations of morphological structure and chemical compositions in the cell wall in a time-dependent manner. Our results confirmed that CRM is a useful tool for the identification of compositional changes in the cell wall caused by fungal infection without the need for any chemical treatment. For the first time, the current research applied CRM in phytopathology for investigating interactive relationship between a pathogen and its host, thus offering a new way for in-depth study of pathogen-host interactions at cellular level.
研究领域:
植物病理学
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光谱学
·
细胞生物学
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采后生理学
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微生物-宿主相互作用
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Xinru Liang,Yali Liu,Changde Song,Jinliang Ning,Chuanfang Zhao,Jiawen Li,Baiyi Zu,Xincun Dou
CNF biomacromolecules were developed as green MOF modulators. • Defective nanosized MOF with enhanced fluorescence activity was successfully prepared. • ICT effect modulation enables sensitive organophosphorus pesticide detection. • Food-grade multifunctional fruit coating was constructed via cellulose self-assembly. • The work offers new insight into cellulose high-value use in food & agricultural safety. Biomass-derived polysaccharide macromolecules have emerged as sustainable, biocompatible functional materials for green agricultural and food applications, owing to their tunable surface groups, self-assembly properties and environmental friendliness. MOFs hold great sensing potential for their superior structural properties, while pure MOFs have critical flaws (severe agglomeration, low mass transfer, poor specificity/stability) in practice, limiting their broader deployment. To address this challenge, a general strategy for structural regulation of UiO-66-OH using green and sustainable carboxylated cellulose nanofibrils (CNFs, a typical linear polysaccharide biomacromolecule) as the sole biogenic modulator and nano-template was proposed. Driven by the competitive coordination and nano-templating effects of CNFs, the defective nanosized UiO-66-OH features abundant active sites and amplified fluorescence signals, modulating the intramolecular charge transfer (ICT) effect for sensitive pesticide detection. In the presence of the target analyte, a distinct fluorescence enhancement was achieved, with an LOD of 1.40 μg/kg (far below the international glyphosate MRL of 0.1 mg/kg in grains) and robust anti-interference against 17 common interferents. Additionally, leveraging the self-assembly properties of CNFs polysaccharide, a food-grade multifunctional fruit coating was fabricated via spray coating, enabling in-situ visual monitoring of pesticide residues and fruit freshness, pesticide degradation, and long-acting fruit preservation. This work offers a rational material design insight and technical support for high-value utilization of cellulose-based biomacromolecules, as well as the green governance of agricultural pesticide residues and high-efficiency fruit preservation. Download: Download high-res image (179KB) Download: Download full-size image
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Foods
·
2026
·Vol. 15
·期 16
·2922
·
IF 6
Sen Huang,Zhihong Lu,Jiying Zhang,Yu Zhang,Zegang Li,Wanfu Yao,Yujiao Cheng
Fruit wines fermented from citrus fruits are highly popular worldwide. The flavor profiles of fruit wines (ELFW, SLFW, PLFW) fermented from three different varieties of lemons (Eureka lemon, ELFW; Sweet lemon, SLFW; and Perfume lemon, PLFW) were investigated by odor activity values combined with multivariate statistical analysis. Volatile compounds in fruit wines were qualitatively and quantitatively analyzed using solid-phase microextraction (SPME) coupled with gas chromatography–mass spectrometry (GC–MS) and multidimensional GC–MS (MDGC–MS). A total of 85 volatile compounds were identified across the samples, with ELFW containing the highest number (67), followed by SLFW (65) and PLFW (58). Alcohols (51.25–73.50%) dominated the volatiles, followed by hydrocarbons and esters. α-Terpineol, 4-terpineol, and d-limonene dominated ELFW, SLFW, and PLFW respectively. Forty-six characteristic flavor compounds were screened, and fenchol showed the highest OAVs. Using OPLS-DA combined with variable importance in projection (VIP), 17 key flavor markers were identified to distinguish flavor difference among three lemon cultivar-based fermented fruit wines.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Yue Yuan,Huafeng Tian,Ruru Huang,Hongtao Liu,Hua Wu,Gaiping Guo,Junsong Xiao
To extend the shelf life of sweet cherries ( Prunus avium L . ) and considering the environmental problems caused by traditional packaging materials, novel Zein/Gelatin-proanthocyanidins-zinc oxide nanoparticles (ZE/GE-PC-ZnO) and Zein/Gelatin-gallic acid-zinc oxide nanoparticles (ZE/GE-GA-ZnO) protein-based composite nanofiber films were prepared by electrospinning. According to the results, ZE/GE-PC-ZnO and ZE/GE-GA-ZnO films' contact angles were higher than those of Zein/Gelatin film by 28.91% and 21.27%, and their antioxidant activities were 5 and 9 times higher, respectively. Moreover, ZE/GE-PC-ZnO film showed good inhibitory activity against B. cinerea . On the eleventh day of the cherry packaging test, compared to unwrapped cherries, the losses of weight and firmness of wrapped fruit were reduced by more than 20% and 60%, respectively. Respiration time was delayed by 5 days, and the peak of ethylene release was decreased by nearly half. In conclusion, these two nanofiber films were viable packaging materials that fulfilled global strategies for green development.
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危险品化学品经营许可证（带存储）