International Journal of Nanomedicine
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2019
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IF 4.471
Wenchao Wu,Jiangqing Wu,Qiafan Fu,Chenhao Jin,Fangyuan Guo,Qinying Yan,Qingliang Yang,Danjun Wu,Yan Yang,Gensheng Yang
Purpose: Clinical applications of curcumin (Cur) have been greatly restricted due to its low solubility and poor systemic bioavailability. Three-arm amphiphilic copolymer tricarballylic acid-poly (ε-caprolactone)-methoxypolyethylene glycol (Tri-CL-mPEG) nanoparticles (NPs) were designed to improve the solubility and bioavailability of Cur. The present study adopted a microchannel system to precisely control the preparation of self-assembly polymeric NPs via liquid flow-focusing and gas displacing method.Methods: The amphiphilic three-arm copolymer Tri-CL-mPEG was synthesized and self-assembled into nearly spherical NPs, yielding Cur encapsulated into NP cores (Cur-NPs). The obtained NPs were evaluated for physicochemical properties, morphology, toxicity, cellular uptake by A549 cells, release in vitro, biodistribution, and pharmacokinetics in vivo.Results: Rapidly fabricated and isodispersed Cur-NPs prepared by this method had an average diameter of 116±3 nm and a polydispersity index of 0.197±0.008. The drug loading capacity and entrapment efficiency of Cur-NPs were 5.58±0.23% and 91.42±0.39%, respectively. In vitro release experiments showed sustained release of Cur, with cumulative release values of 40.1% and 66.1% at pH 7.4 and pH 5.0, respectively, after 10 days post-incubation. The results of cellular uptake, biodistribution, and in vivo pharmacokinetics experiments demonstrated that Cur-NPs exhibited better biocompatibility and bioavailability, while additionally enabling greater cellular uptake and prolonged circulation with possible spleen, lung, and kidney targeting effects when compared to the properties of free Cur.Conclusion: These results indicate that Tri-CL-mPEG NPs are promising in clinical applications as a controllable delivery system for hydrophobic drugs.
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Nutrients
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2018
·Vol. 10
·期 7
·855
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IF 4.196
Mohammad Hosein Farzaei,Mahdi Zobeiri,Fatemeh Parvizi,Fardous F. El-Senduny,Ilias Marmouzi,Ericsson Coy-Barrera,Rozita Naseri,Seyed Mohammad Nabavi,Roja Rahimi,Mohammad Abdollahi
Oxidative stress has been considered a key causing factor of liver damage induced by a variety of agents, including alcohol, drugs, viral infections, environmental pollutants and dietary components, which in turn results in progression of liver injury, non-alcoholic steatohepatitis, non-alcoholic liver disease, liver fibrosis and cirrhosis. During the past 30 years and even after the major progress in the liver disease management, millions of people worldwide still suffer from an acute or chronic liver condition. Curcumin is one of the most commonly used indigenous molecules endowed by various shielding functionalities that protects the liver. The aim of the present study is to comprehensively review pharmacological effects and molecular mechanisms, as well as clinical evidence, of curcumin as a lead compound in the prevention and treatment of oxidative associated liver diseases. For this purpose, electronic databases including “Scopus,” “PubMed,” “Science Direct” and “Cochrane library” were extensively searched with the keywords “curcumin or curcuminoids” and “hepatoprotective or hepatotoxicity or liver” along with “oxidative or oxidant.” Results showed that curcumin exerts remarkable protective and therapeutic effects of oxidative associated liver diseases through various cellular and molecular mechanisms. Those mechanisms include suppressing the proinflammatory cytokines, lipid perodixation products, PI3K/Akt and hepatic stellate cells activation, as well as ameliorating cellular responses to oxidative stress such as the expression of Nrf2, SOD, CAT, GSH, GPx and GR. Taking together, curcumin itself acts as a free radical scavenger over the activity of different kinds of ROS via its phenolic, β-diketone and methoxy group. Further clinical studies are still needed in order to recognize the structure-activity relationships and molecular mechanisms of curcumin in oxidative associated liver diseases.Keywords:curcumin;hepatotoxicity;liver diseases;oxidative stress;systematic review
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Analytical Methods
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2014
·Vol. 6
·期 18
·7496-7501
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IF 1.938
Xiaoqian Ouyang,Yaping Ding,Xi Ba,Shuqing Gu
A poly-Ni(II)–curcumin (curcumin: 1,7-bis[4-hydroxy-3-methoxyphenyl]-1,6-heptadiene-3,5-dione) composite film modified electrode was prepared for amperometric determination of β-nicotinamide adenine dinucleotide (NADH). Scanning electron microscopy and Energy Dispersive X-ray spectroscopy were used to examine the morphology and interface property of the Ni(II)–curcumin film. Under optimum conditions, the amperometric detection of NADH provided a wide linear detection range (0.3–300 μM), high sensitivity (230.02 μA mM−1 cm−2) and low limit of detection (LOD = 0.18 μM, S/N = 3). Moreover, the modified electrode showed good stability and reproducibility, indicating that it can improve the analysis signal of NADH in a very convenient way.
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LUMINESCENCE
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2017
·Vol. 33
·期 1
·174-180
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IF 1.509
Wei Bian,Xuan Wang,Yakun Wang,Haifen Yang,Jialin Huang,Zongwei Cai,Martin M. F. Choi
In this present study, a fluorescent probe was developed to detect curcumin, which is derived from the rhizomes of the turmeric. We used a simple and economical way to synthesize boron and nitrogen co-doped carbon dots (BNCDs) by microwave heating. The maximum emission wavelength of the BNCDs was 450 nm at an excitation wavelength of 360 nm. The as-prepared BNCDs were characterized by multiple analytical techniques such as transmission electron microscopy, X-ray photoelectron spectroscopy, X-ray diffraction, and infrared spectroscopy. The synthesized carbon nanoparticles had an average particle diameter of 4.23 nm. The BNCDs exhibited high sensitivity to the detection of curcumin at ambient conditions. The changes of BNCDs fluorescent intensity show a good linear relationship with the curcumin concentrations in the range 0.2–12.5 μM. This proposed method has been successfully applied to detect the curcumin in urine samples with the recoveries of 96.5–105.5%.
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RSC Advances
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2017
·Vol. 7
·期 27
·16401-16409
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IF 3.108
Xian Sun,Yingping Luo,Liwei Huang,Bo-Yang Yu,Jiangwei Tian
Multidrug resistance (MDR) is a major obstacle to the success of cancer chemotherapy, which is closely associated with the overexpression of P-glycoprotein (P-gp) and the endolysosomal capture of drug. Therefore, to explore a strategy that could down-regulate P-gp expression and promote drugs escaping from the endolysosome is highly desired for overcoming MDR in chemotherapeutic treatment. This work reports a peptide-functionalized mesoporous silica nanomedicine (MSN) for encapsulation of curcumin (CUR) and doxorubicin (DOX) to significantly reverse the MDR in consequence of rapid endolysosomal escape and the inhibition of P-gp function. The nanomedicine is modified with the peptide of GFLGHHHRRGDS to encapsulate CUR and DOX with high loading efficiency and content. It can be selectively uptaken by the DOX resistant MCF-7/ADR cells via the specific recognition between RGDS and αvβ3 integrin to enter into lysosomes. Afterwards, the GFLG is cleaved by the lysosomal cathepsin B to release CUR and DOX from MSN. Furthermore, the imidazole-rich HHHR peptide can induce effective lysosomal membrane permeability which is beneficial for drug escape from lysosome, CUR-mediated inhibition of P-gp, and nuclear import of DOX in MCF-7/ADR cells. Confocal fluorescence imaging, flow cytometry and MTT assays demonstrate that the combination of CUR and the peptide-functionalized MSN markedly enhances the intracellular DOX concentration and achieves a successful chemotherapeutic treatment to MCF-7 or MCF-7/ADR cells, thus this work offers an effective strategy to overcome MDR.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2017
·
IF 3.671
Yurui Xu,Sajid Asghar,Liu Yang,Zhipeng Chen,Hongying Li,Wenwen Shi,Yibo Li,Qinqing Shi,Qineng Ping,Yanyu Xiao
Nanoparticles based on chitosan hydrochloride (CSH)-hyaluronic acid (HA)-PEG were prepared for delivering curcumin (CUR) (CUR-PNPs) to brain tumor. CUR-PNPs of 245.9 nm and spherical morphology were obtained at optimized CSH/HA/PEG20000/CUR ratios with negative charge of about −27.2 mV and EE of approximately 93.3%. Cytotoxicity studies showed that CUR-PNPs improved drug’s anticancer activity in rat glioma cells (C6). The cellular uptake mechanism study showed active targeting of CUR-PNPs into C6 cells by HA mediated endocytosis . Clathrin-coated pit mediated endocytosis, clathrin-mediated endocytosis and macropincytosis were also identified as the entry pathways of PNPs into C6 cells. Pharmacokinetics of preparations in rats after i.v. administration further proved the superiority of CUR-PNPs (3.98 times greater than the area under the curve of CUR solution). In conclusion, CUR-PNPs might be a promising carrier for the therapy of brain tumors.
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International Journal of Nanomedicine
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2017
·
IF 4.3
Wen-Ting Zhu,Sheng-Yao Liu,Lei Wu,Hua-Li Xu,Jun Wang,Guo-Xin Ni,Qing-Bing Zeng
Background It has been widely reported that curcumin (CUR) exhibits anticancer activity and triggers the apoptosis of human A549 non-small-cell lung cancer (NSCLC) cells. However, its application is limited owing to its poor solubility and bioavailability. Therefore, there is an urgent need to develop a new CUR formulation with higher water solubility and better biocompatibility for clinical application in the future. Materials and methods In this study, CUR-loaded methoxy polyethylene glycol–polylactide (CUR/mPEG–PLA) polymeric micelles were prepared by a thin-film hydration method. Their characteristics and antitumor effects were evaluated subsequently. Results The average size of CUR/mPEG–PLA micelles was 34.9±2.1 nm with its polydispersity index (PDI) in the range of 0.067–0.168. The encapsulation efficiency and drug loading were 90.2%±0.78% and 9.1%±0.07%, respectively. CUR was constantly released from the CUR/mPEG–PLA micelles, and its cellular uptake in A549 cells was significantly increased. It was also found that CUR/mPEG–PLA micelles inhibited A549 cell proliferation, increased the cell cytotoxicity, induced G2/M stage arrest and promoted cell apoptosis. Moreover, the CUR/mPEG–PLA micelles suppressed the migration and invasion of A549 cells more obviously than free CUR. Additionally, CUR/mPEG–PLA micelles inhibited human umbilical vein endothelial cells migration, invasion and corresponding tube formation, implying the antiangiogenesis ability. Its enhanced antitumor mechanism may be related to the reduced expression of vascular endothelial growth factor, matrix metalloproteinase (MMP)-2, MMP-9 and Bcl-2 as well as the increased expression of Bax. Conclusion The mPEG–PLA copolymer micelles can serve as an efficient carrier for CUR. The CUR/mPEG–PLA micelles have promising clinical potential in treating NSCLC.
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BIOMEDICINE & PHARMACOTHERAPY
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2017
·
IF 2.759
Fangfang Teng,Peizong Deng,Zhimei Song,Feilong Zhou,Runliang Feng
In order to enhance water-solubility and realize controlled release while keeping synergistic effects of ketoconazole and curcumin , drug-loaded methoxy poly (ethylene glycol)-b-poly (ε-caprolactone) micelles were prepared through thin membrane hydration method. Transmission electric microscopy and dynamitic light scattering characterization revealed the formation of ketoconazole- and curcumin-loaded micelles with an average size of 44.70 nm and 39.56 nm, respectively. The drug-loaded micelles endowed the two drugs’ slow controlled release with water-solubility enhanced to 85 and 82000 folds higher than the corresponding raw drugs, respectively. In vitro antifungal activity test, chequerboard test and inhibition zone test indicated that efficacy of ketoconazole-loaded micelles was improved by introduction of curcumin-loaded micelles with a low fractional inhibitory concentration index (0.073). Biofilm formation inhibition assay also demonstrated that participation of curcumin-loaded micelles obviously strengthened the inhibition of fungal biofilms formation induced by ketoconazole-loaded micelles. The high synergistic activity of combinations is encouraging and the MPEG-PCL micelle is a potential drug delivery system for the combination of ketoconazole and curcumin.
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FOOD RESEARCH INTERNATIONAL
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2025
·
IF 8
Hang Liu,Yikun Liu,Shengfeng Peng,Lei Zhou,David Julian McClements,Suqiong Fang,Wei Liu
In this study, the application of banana-derived extracellular vesicles (EVs) as natural carriers for hydrophobic nutraceuticals was investigated for the first time. Curcumin was solubilized in an amorphous state within the hydrophobic domains of the EVs in banana juice. The bioaccessibility of the curcumin was considerably higher in the curcumin-loaded EVs (CEVs) than for pure curcumin crystals. The retention/release behavior of the curcumin in the CEVs was regulated by incorporating the curcumin-loaded banana juice into hydrogel beads, which were assembled from sodium alginate and banana pectin. The pectin and D-galacturonic acid contents of the banana juice were 0.87 % ± 0.11 % and 0.58 % ± 0.08 %, respectively. With the introduction of sodium alginate, the sphericity, hardness, chewiness, water holding capacity, surface smoothness and other physiochemical properties of the hydrogel beads were improved. Fourier Transform infrared analysis showed that hydrogen bonding played an important role in the formation of the hydrogel beads. The hydrogel beads had a higher swelling ratio under neutral conditions than under acidic conditions. Encapsulation of the curcumin-loaded banana juice within the hydrogel beads retarded curcumin release and enhanced the therapy potential of ulcerative colitis, which indicated that these delivery systems could be designed to regulate the release profile of the CEVs. Encapsulation of the curcumin also improved its resistance to heating, exposure and storage. In summary, we have shown that encapsulation of curcumin within plant-based EVs loaded into hydrogel beads can improve its functional performance.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2024
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IF 4.9
Liyin Zhang,Shanshan Dai,Li Zhou,Xia Liu,Yuanqiang Zhu,Xiaowen Zhao
This study aimed to develop a surfactant-free microemulsion (SFME) which based on hydrophobic deep eutectic solvent (DES), intended to prospect its extraction effect of curcuminoids. A menthol and n-octanoic acid (ME-OA 1:2) hydrophobic DES is used as oil phase to SFME which have low viscosity, higher stability and better solubility for extracting curcuminoids. The hydrophobic DES was successfully formed which was demonstrated by 1HNMR and FT-IR. The ternary phase diagram and the microstructure of microemulsions was determined via visual titration, light scattering experiment and electrical conductivity. SFME consisting of ME-OA/Ethanol/H 2 O (1/7/2 mass ratio) had been constructed to investigate its ability to extract curcuminoids from turmeric rhizome utilizing HPLC. And the it was tested the extracted curcuminoids as a function of extraction time by UV–vis spectra. Using the single-factor experiments and response surface methodology (RSM), the optimal extraction conditions by ultrasound-assisted SFME were identified as follows: liquid/solid ratio of 12 g/g, extraction time of 63 min, and ultrasonic time (300 W) of 45 min. The total extraction amounts of curcuminoids could reach 61.39 mg/g powder under the best conditions, and the main factor to influence the final extraction effect was extraction time. The molecular dynamic simulation was performed to investigate the interaction between SFME and curcuminoids. Compared with the single component solvent system of ethanol, acetone, ME-OA and water, curcuminoids were stronger interaction energy with solvent molecules and higher hydrogen bond number in SFME. SFME formed the O/W structure of encapsulation solubilization for curcuminoids, in order to promote the dissolving of curcuminoids.
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INTERNATIONAL DAIRY JOURNAL
·
2024
·
IF 3.1
Zhiwen Ge,Dan Wang,Mingsheng Dong,Wenting Zhao,Pan Wang,Peiyou Qin,Xiaoyan Zhao
The functional properties of foods are closely related with its matrix. This study examined the impact of curcumin-fortified Lactobacillus helveticus MB2-1 fermented milk gel on the intestinal lipid digestion and bioaccessibility of curcumin through dynamic digestion method. The gel properties, microstructure, oil droplets stability, free fatty acid (FFA) release and curcumin bioaccessibility during digestion of the curcumin-fortified milk gels fermented by L. helveticus MB2-1 or tradition commercial starters ( Streptococcus thermophilus and Lactobacillus bulgaricus ) were investigated and compared. The curcumin-fortified L. helveticus MB2-1 fermented milk gel was found to present higher apparent viscosity, more thicker properties, and firmer texture, which affect the gastrointestinal digesta status together with the release of oil droplets and solubilized curcumin, promote lipid digestion as well as enhance the bioaccessibility of curcumin during digestion. These results may provide valuable references for the design of therapeutic dairy products beneficial to health in terms of food matrix.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Ruoyuan Huang,Wenjie Sun,Wenzhao Li,Rui Hu,Rui Meng,Zheng Peng,Rongbo Yang,Tianyu Huang,Jinhui Du,Luoran Shang,Congying Xie
Radiation dermatitis, as a major side effect of radiotherapy, is a form of skin injury linked to the production of reactive oxygen species (ROS). Development of functional patches with excellent ROS scavenging ability and biosafety is crucial for radioactive dermatitis repair. Herein, we present a nanohybrid hydrogel patch composed with natural-derived substances. The patch possesses dual functionalities of chemically scavenging ROS and physically absorbing radiation, thereby alleviating radiation damage. Curcumin@tannic acid (Cur@TA) nanoparticles are synthesized through spontaneous coordination of TA and iron ions (Fe 3+ ), enabling the encapsulation of Cur. The nanoparticles are then loaded into methacrylate gelatin (GelMA) hydrogel patches, which endow the hydrogel with tissue adhesion as well as photothermal properties. Simultaneously, the patch exhibits powerful ROS scavenging capability and promotes M2 polarization of macrophages. Additionally, the high-water content of the GelMA hydrogel enables physical absorption of low-energy X-rays, providing radiation shielding. Based on these characteristics, we have demonstrated the augmented therapeutic efficacy of the hydrogel patch loaded with Cur@TA nanoparticles in the mouse model of radiation dermatitis. Our findings introduce a novel therapeutic approach for radiation protection and treatment, with potential clinical applications.
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