Analytical Methods
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2014
·Vol. 6
·期 18
·7496-7501
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IF 1.938
Xiaoqian Ouyang,Yaping Ding,Xi Ba,Shuqing Gu
A poly-Ni(II)–curcumin (curcumin: 1,7-bis[4-hydroxy-3-methoxyphenyl]-1,6-heptadiene-3,5-dione) composite film modified electrode was prepared for amperometric determination of β-nicotinamide adenine dinucleotide (NADH). Scanning electron microscopy and Energy Dispersive X-ray spectroscopy were used to examine the morphology and interface property of the Ni(II)–curcumin film. Under optimum conditions, the amperometric detection of NADH provided a wide linear detection range (0.3–300 μM), high sensitivity (230.02 μA mM−1 cm−2) and low limit of detection (LOD = 0.18 μM, S/N = 3). Moreover, the modified electrode showed good stability and reproducibility, indicating that it can improve the analysis signal of NADH in a very convenient way.
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RSC Advances
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2017
·Vol. 7
·期 42
·25978-25986
·
IF 3.108
Ce Cheng,Shengfeng Peng,Ziling Li,Liqiang Zou,Wei Liu,Chengmei Liu
The poor water solubility and bioavailability of curcumin can be improved by encapsulating it into liposomes. However, the existing encapsulation technologies, such as the thin film method and the ethanol injection method, are complex and require the use of organic solvents. In this study, an organic solvent-free and easily scalable encapsulation technique was studied by utilizing the pH-dependent solubility properties of curcumin. Phospholipid was dissolved in water to form liposomes. Curcumin was deprotonated and dissolved under alkaline conditions and then encapsulated into the liposomes after acidification. Morphological observation and X-ray diffraction analysis confirmed that curcumin liposomes had been successfully prepared. Curcumin liposomes prepared by the pH-driven method were stable during storage. During in vitro digestion, curcumin liposomes prepared by the pH-driven method showed similar bioaccessibility to those prepared by the thin film method and higher bioaccessibility than those prepared by the ethanol injection method. The pH-driven method, which is organic solvent-free and easily scalable for industrial production, is thus a promising method for the preparation of curcumin liposomes.
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Polymers
·
2025
·Vol. 17
·期 7
·830
·
IF 4.9
Hashimu Juma,Cunshi Zhao,Qingbo Wang,Yunfeng Guo,Xinyan Fan,Wuming Fan,Linlin Zhao,Jiayi Sun,Dong Wang,Yonggui Wang
Polybutylene adipate-terephthalate (PBAT) offers a convincing ecological alternative to the traditional fossil-based plastics due to its biodegradability and robust mechanical properties. The objective of this study is to develop PBAT-based bio-composite films through incorporating functionalized cellulose nanocrystals (CNC) and curcumin (CUR). In order to improve the interfacial compatibility with the PBAT matrix and co-doping with CUR, CNC was modified using dodecyl succinic anhydride (DxCNC). In this ternary bio-composite system, CUR functioned as a bio-based antioxidant and antimicrobial agent. The presence of CUR also provides excellent UV-shielding properties, whereas the DxCNC effectively enhances the controlled release of CUR. The synergistic effect between DxCNC and CUR in boosting antimicrobial properties, with the inhibition values forE. coliandS. aureusreached 1.82 log CFU/cm2and 2.12 log CFU/cm2, respectively. These findings indicate DxCNC/CUR/PBAT ternary composite films as a promising material for eco-friendly packaging products.
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2024
·
IF 5.4
Mengyi Zhang,Ju Liang,Ying Liang,Xuening Li,Wenlan Wu
In the realm of intracellular drug delivery, overcoming the barrier of endosomal entrapment stands as a critical factor influencing the effectiveness of nanodrug delivery systems. This study focuses on the synthesis of an acid-sensitive fatty acid derivative called imidazole-stearic acid (IM-SA). Leveraging the proton sponge effect attributed to imidazole groups, IM-SA was anticipated to play a pivotal role in facilitating endosomal escape. Integrated into the lipid core of solid lipid nanoparticles (SLNs), IM-SA was paired with hyaluronic acid (HA) coating on the surface of SLNs loading with curcumin (CUR). The presence of IM-SA and HA endowed HA-IM-SLNs@CUR with dual functionalities, enabling the promotion of endosomal escape, and specifical targeting of liver cancer. HA-IM-SLNs@CUR exhibited a particle size of ~228 nm, with impressive encapsulation efficiencies (EE) of 87.5% ± 2.3% for CUR. Drugs exhibit significant pH sensitive release behavior. Cellular experiments showed that HA-IM-SLN@CUR exhibits enhanced drug delivery capability. The incorporation of IM-SA significantly improved the endosomal escape of HA-IM-SLN@CUR, facilitating accelerated intracellular drug release and increasing intracellular drug concentration, exhibiting excellent growth inhibitory effects on HepG2 cells. Animal experiments revealed a 3.4-fold increase in CUR uptake at the tumor site with HA-IM-SLNs@CUR over the free CUR, demonstrating remarkable tumor homing potential with the tumor growth inhibition rate of 97.2%. These findings indicated the significant promise of HA-IM-SLNs@CUR in the realm of cancer drug delivery.
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Scientific Reports
·
2024
·Vol. 14
·期 1
·1-15
·
IF 3.8
Chen Linli,Guo Xiaojing,Lin Wen,Huang Yingying,Zhuang Suling,Li Qianfeng,Xu Jianhua,Ye Shengnan
Lytic induction therapy was devised to selectively combat malignancies associated with Epstein–Barr virus (EBV) by triggering viral reactivation from latency. At present, the major challenges of lytic induction therapy are to maximize reactivating efficiencies and meanwhile minimize infectious virion production. C210, a novel curcumin derivative with potent Hsp90 inhibitory activity, was explored for EBV-reactivating and virion-producing effects in EBV-positive nasopharyngeal carcinoma (NPC) and gastric carcinoma (GC) cell lines. And the molecular mechanisms underlying these effects were determined. Follow C210 treatment, EBV lytic RNAs and proteins were upregulated, but infectious virions were not produced. Knockdown of heat shock protein 90 (Hsp90) induced expression of lytic RNAs and proteins, and diminished C210-driven EBV lytic induction. Pretreatment with an X box binding protein 1 (XBP1) inhibitor reduced C210-induced EBV lytic RNA. Furthermore, we demonstrated that C210 inhibited the binding of Hsp90 with its clients, signal transducer and activator of transcription 3 (STAT3) and xeroderma pigmentosum group B-complementing protein (XPB), which subsequently promoted their proteasomal degradation. Degradation of STAT3 by C210 enhanced the EBV-reactivating and anticancer capacity of suberoylanilide hydroxamic acid (SAHA). Depletion of XPB blocked SAHA-induced expression of late viral genes and production of infectious virions. These results elucidate a novel Hsp90 inhibitor targeting EBV lytic phase and extend the research on lytic induction strategy, which may offer reference value in the treatment of EBV-positive malignancies.
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INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Wei Tan,Yin Tian,Chunlong Zuo,Yongli Zhang,Xiaoyu Liu,Ziyin Ye,Lu Bai,Yuanlin Ren,Xiaohui Liu
With the depletion of petrochemical resources and the escalating severity of white pollution, replacing petrochemical resources with bio-based resources aligns with sustainable development strategy. In this work, a novel biomass flame retardant (PCDAC) derived from curcumin was intercalated into expandable graphite (EG) to construct an intrinsically intumescent flame retardant system (EG@PCDAC), which was then melt blended with polylactic acid (PLA) to fabricate flame retardant, anti-dripping and smoke suppressive PLA composite (PLA/EG@PCDAC). The residual char of PLA/EG@PCDAC was 14.27 wt% under N 2 atmosphere, while reaching UL-94 V–0 rating. Furthermore, the peak heat release rate and total heat release of PLA/EG@PCDAC were reduced by 46.95 % and 19.02 % compared with original PLA, and the total smoke production and peak smoke production rate also decreased by 20.74 % and 72.09 %, respectively. This work offered green and feasible inspiration for exploring biomass flame retardants and renewable PLA-based functional materials.
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INTERNATIONAL DAIRY JOURNAL
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2024
·
IF 3.1
Zhiwen Ge,Dan Wang,Mingsheng Dong,Wenting Zhao,Pan Wang,Peiyou Qin,Xiaoyan Zhao
The functional properties of foods are closely related with its matrix. This study examined the impact of curcumin-fortified Lactobacillus helveticus MB2-1 fermented milk gel on the intestinal lipid digestion and bioaccessibility of curcumin through dynamic digestion method. The gel properties, microstructure, oil droplets stability, free fatty acid (FFA) release and curcumin bioaccessibility during digestion of the curcumin-fortified milk gels fermented by L. helveticus MB2-1 or tradition commercial starters ( Streptococcus thermophilus and Lactobacillus bulgaricus ) were investigated and compared. The curcumin-fortified L. helveticus MB2-1 fermented milk gel was found to present higher apparent viscosity, more thicker properties, and firmer texture, which affect the gastrointestinal digesta status together with the release of oil droplets and solubilized curcumin, promote lipid digestion as well as enhance the bioaccessibility of curcumin during digestion. These results may provide valuable references for the design of therapeutic dairy products beneficial to health in terms of food matrix.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2024
·
IF 4.9
Liyin Zhang,Shanshan Dai,Li Zhou,Xia Liu,Yuanqiang Zhu,Xiaowen Zhao
This study aimed to develop a surfactant-free microemulsion (SFME) which based on hydrophobic deep eutectic solvent (DES), intended to prospect its extraction effect of curcuminoids. A menthol and n-octanoic acid (ME-OA 1:2) hydrophobic DES is used as oil phase to SFME which have low viscosity, higher stability and better solubility for extracting curcuminoids. The hydrophobic DES was successfully formed which was demonstrated by 1HNMR and FT-IR. The ternary phase diagram and the microstructure of microemulsions was determined via visual titration, light scattering experiment and electrical conductivity. SFME consisting of ME-OA/Ethanol/H 2 O (1/7/2 mass ratio) had been constructed to investigate its ability to extract curcuminoids from turmeric rhizome utilizing HPLC. And the it was tested the extracted curcuminoids as a function of extraction time by UV–vis spectra. Using the single-factor experiments and response surface methodology (RSM), the optimal extraction conditions by ultrasound-assisted SFME were identified as follows: liquid/solid ratio of 12 g/g, extraction time of 63 min, and ultrasonic time (300 W) of 45 min. The total extraction amounts of curcuminoids could reach 61.39 mg/g powder under the best conditions, and the main factor to influence the final extraction effect was extraction time. The molecular dynamic simulation was performed to investigate the interaction between SFME and curcuminoids. Compared with the single component solvent system of ethanol, acetone, ME-OA and water, curcuminoids were stronger interaction energy with solvent molecules and higher hydrogen bond number in SFME. SFME formed the O/W structure of encapsulation solubilization for curcuminoids, in order to promote the dissolving of curcuminoids.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Ruoyuan Huang,Wenjie Sun,Wenzhao Li,Rui Hu,Rui Meng,Zheng Peng,Rongbo Yang,Tianyu Huang,Jinhui Du,Luoran Shang,Congying Xie
Radiation dermatitis, as a major side effect of radiotherapy, is a form of skin injury linked to the production of reactive oxygen species (ROS). Development of functional patches with excellent ROS scavenging ability and biosafety is crucial for radioactive dermatitis repair. Herein, we present a nanohybrid hydrogel patch composed with natural-derived substances. The patch possesses dual functionalities of chemically scavenging ROS and physically absorbing radiation, thereby alleviating radiation damage. Curcumin@tannic acid (Cur@TA) nanoparticles are synthesized through spontaneous coordination of TA and iron ions (Fe 3+ ), enabling the encapsulation of Cur. The nanoparticles are then loaded into methacrylate gelatin (GelMA) hydrogel patches, which endow the hydrogel with tissue adhesion as well as photothermal properties. Simultaneously, the patch exhibits powerful ROS scavenging capability and promotes M2 polarization of macrophages. Additionally, the high-water content of the GelMA hydrogel enables physical absorption of low-energy X-rays, providing radiation shielding. Based on these characteristics, we have demonstrated the augmented therapeutic efficacy of the hydrogel patch loaded with Cur@TA nanoparticles in the mouse model of radiation dermatitis. Our findings introduce a novel therapeutic approach for radiation protection and treatment, with potential clinical applications.
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CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Wenjuan Wang,Suping Ji,Qiang Xia
Recently, there has been a focus on using biopolymer-based particles to stabilize high internal phase Pickering emulsions (HIPPEs) due to the notable advances in biocompatibility and biodegradability. In this work, the complex particles of peanut protein isolate and carboxymethyl cellulose (CMC) with various substitution degrees (DS; 0.7 and 0.9) and weight average molecular weights (Mw; 90, 250, and 700 kDa) were prepared and characterized as novel stabilizers. For the obtained four types of morphologically distinct particles, the complex particles formed by CMC (0.9 DS and 250 kDa) showed cluster structures with an average size of 1.271 μm, equally biphasic wettability with three-phase contact angles of 91.5°, and the highest diffusion rate at the oil-water interface. HIPPEs stabilized by these particles exhibited more elastic behavior due to the smaller tanδ and higher viscosity, as well as excellent thixotropic recovery properties and stability against heating, storage, and freeze-thawing. Furthermore, confocal laser scanning microscopy verified that these particles formed a dense interfacial layer around the oil droplets, which could resist flocculation and coalescence between oil droplets during in vitro digestion. The improved bioaccessibility of curcumin-loaded HIPPEs made these delivery systems potentially apply in functional foods.
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JOURNAL OF PHARMACEUTICAL SCIENCES
·
2024
·
IF 3.7
Jiawei Han,Yang Yang,Yunjuan Hou,Mengyuan Tang,Yunran Zhang,Yijun Zhu,Xiaoqian Liu,Jue Wang,Yuan Gao
Intermolecular interactions between drug and co-former are crucial in the formation, release and physical stability of co-amorphous system. However, the interactions remain difficult to investigate with only experimental tools. In this study, intermolecular interactions of co-amorphous curcumin-piperine (i.e., CUR-PIP CM) during formation, dissolution and storage were explored by integrating experimental and modeling techniques. The formed CUR-PIP CM exhibited the strong hydrogen bond interaction between the phenolic OH group of CUR and the C O group of PIP as confirmed by FTIR, ss 13 C NMR and molecular dynamics (MD) simulation. In comparison to crystalline CUR, crystalline PIP and their physical mixture, CUR-PIP CM performed significantly increased dissolution accompanied by the synchronized release of CUR and PIP, which arose from the greater interaction energy of H 2 O-CUR molecules and H 2 O-PIP molecules than CUR-PIP molecules, breaking the hydrogen bond between CUR and PIP molecules, and then causing a pair-wise solvation of CUR-PIP CM at the molecular level. Furthermore, the stronger intermolecular interaction between CUR and PIP was revealed by higher binding energy of CUR-PIP molecules, which contributed to the excellent physical stability of CUR-PIP CM over amorphous CUR or PIP. The study provides a unique insight into the formation, release and stability of co-amorphous system from MD perspective. Meanwhile, this integrated technique can be used as a practical methodology for the future design of co-amorphous formulations.
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ACS Nano
·
2024
·
IF 15.8
Yixuan Liu,Feng Lin,Cheng Wu,Wanguo Liu,Hao Wang,Chunsheng Xiao,Xuesi Chen
The microenvironment after traumatic spinal cord injury (SCI) involves complex pathological processes, including elevated oxidative stress, accumulated reactive aldehydes from lipid peroxidation, excessive immune cell infiltration, etc. Unfortunately, most of current neuroprotection therapies cannot cope with the intricate pathophysiology of SCI, leading to scant treatment efficacies. Here, we developed a facile in situ reaction-induced self-assembly method to prepare aldehyde-scavenging polypeptides (PAH)-curcumin conjugate nanoassemblies (named as PFCN) for combined neuroprotection in SCI. The prepared PFCN could release PAH and curcumin in response to oxidative and acidic SCI microenvironment. Subsequently, PFCN exhibited an effectively neuroprotective effect through scavenging toxic aldehydes as well as reactive nitrogen and oxygen species in neurons, modulating microglial M1/M2 polarization, and down-regulating the expression of inflammation-related cytokines to inhibit neuroinflammation. The intravenous administration of PFCN could significantly ameliorate the malignant microenvironment of injured spinal cord, protect the neurons, and promote the motor function recovery in the contusive SCI rat model.
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