CHEMICAL ENGINEERING JOURNAL
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2022
·
IF 16.744
Dian Dai,Mingxiang Qv,Dongyang Liu,Chunming Tang,Wei Wang,Qirui Wu,Zhihong Yin,Liandong Zhu
Magnetic harvesting of microalgae depends on the interface interactions between microalgal cells and flocculants, and thus it is important to find a reasonable evaluation method understand these interface interactions. In this effort, we discussed the formation mechanism and pH regulation of microalgae magnetic harvesting via magnetic chitosan (Fe 3 O 4 @SiO 2 -CS) synthesized by a micro-emulsion method. The flocculation performances and mechanisms during the harvesting of microalgae by the novel flocculant were evaluated. The synthesis process and structure were determined by XRD, FT-IR, SEM and XPS, and the results indicated that Fe 3 O 4 @SiO 2 -CS was successfully prepared. The collection efficiency of 96.52 % was achieved for Chlorella vulgaris within 3 min using Fe 3 O 4 @SiO 2 -CS with the Fe 3 O 4 @SiO 2 and CS mass ratio of 1:3 under pH 1.0. Moreover, this study introduced the two-dimension fractal dimension and extended Derjaguin-Landau-Verwey-Overbeek (E-DLVO) model to evaluate the harvesting performances and mechanisms of Fe 3 O 4 @SiO 2 -CS. Experimental results showed that a strong electrostatic interaction was formed under acidic conditions between Fe 3 O 4 @SiO 2 -CS and microalgae cells with D 2 = 1.82 (fractal dimension value). The hydrophobic tryptophan-like amino acid was observed in the slime-extracellular polymeric substances (S-EPS) secreted by microalgae. S-EPS was found to be a significant substance to promote harvesting performance when the system was neutral or alkaline. This work provides deeper insights into microalgae harvesting by Fe 3 O 4 @SiO 2 -CS as well as reasonable mechanisms to illustrate the interaction of Fe 3 O 4 @SiO 2 -CS and microalgae under different pH values.
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高影响因子
MOLECULES
·
2022
·Vol. 27
·期 11
·3593
·
IF 4.927
Meng Wei,Xiaochang Liu,Peng Xie,Yuanhua Lei,Haojie Yu,Aiyun Han,Libin Xie,Hongliang Jia,Shaohua Lin,Yueyu Bai,Baozhong Sun,Songshan Zhang
This study focused on characterizing the volatile profiles and contributing compounds in pan-fried steaks from different Chinese yellow cattle breeds. The volatile organic compounds (VOCs) of six Chinese yellow cattle breeds (bohai, jiaxian, yiling, wenshan, xinjiang, and pingliang) were analyzed by GC-Q-Orbitrap spectrometry and electronic nose (E-nose). Multivariate statistical analysis was performed to identify the differences in VOCs profiles among breeds. The relationship between odor-active volatiles and sensory evaluation was analyzed by partial least square regression (PLSR) to identify contributing volatiles in pan-fried steaks of Chinese yellow cattle. The results showed that samples were divided into two groups, and 18 VOCs were selected as potential markers for the differentiation of the two groups by GC-Q-Orbitrap combined multivariate statistical analysis. YL and WS were in one group comprising mainly aliphatic compounds, while the rest were in the other group with more cyclic compounds. Steaks from different breeds were better differentiated by GC-Q-Orbitrap in combination with chemometrics than by E-nose. Six highly predictive compounds were selected, including 3-methyl-butanal, benzeneacetaldehyde, 2-ethyl-6-methyl-pyrazine, 2-acetylpyrrole, 2-acetylthiazole, and 2-acetyl-2-thiazoline. Sensory recombination difference and preference testing revealed that the addition of highly predictive compounds induced a perceptible difference to panelists. This study provides valuable data to characterize and discriminate the flavor profiles in pan-fried steaks of Chinese yellow cattle.Keywords:Chinese yellow cattle;volatile organic compounds;GC-Q-Orbitrap;E-nose;sensory evaluation;multivariate statistical analysis
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
·
IF 8.025
Qiang Chen,Chunjing Guo,Xiudi Zhou,Yanguo Su,Huimin Guo,Min Cao,Jing Li,Yue Zhang,Weiyi Zhao,Xin Gao,Shuqi Mi,Daquan Chen
Currently, very limited therapeutic approaches are available for the drug treatment of atherosclerosis(AS). H 2 S-donor is becoming a common trend in much life-threatening research. Several studies have documented that H 2 S-lyase is predominantly present in endothelial cells. N-Acetylneuraminic acid (SA), natural carbohydrate, binds specifically to the E -selectin receptor of endothelial cells. Meanwhile, recent studies related to Chondroitin sulfate have excellent target binding ability with CD44 receptor. We conjecture that the N-Acetylneuraminic acid and Chondroitin sulfate modified nanomicelles not only enhances the accumulation of the drug but also cleaves the H 2 S donor in the lesion, thus one stone two birds. Given these findings, we synthesized two kinds of nanoparticles, Carrier I (SCCF) and Carrier II (SCTM), for atherosclerosis to validate our guesses. Initially, S-allyl-L-cysteine and 4-methoxyphenylthiourea were used as H 2 S donors for SCCF and SCTM, respectively. After the introduction of ROS-sensitive groups. Then, micelles with N-Acetylneuraminic acid and Chondroitin sulfate were prepared to load rapamycin(RAP). Further, in atherosclerosis Oil Red O staining (ORO) results confirmed remarkable treatment effect with [email protected] and [email protected] Thus, we conclude that the effect of dual-targeting nanomicelles with ROS-sensitive H 2 S donor based on N-Acetylneuraminic acid and Chondroitin sulfate will have a better role in atherosclerosis.
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BIORESOURCE TECHNOLOGY
·
2014
·
IF 5.039
Yu-Cai He,Dan-Ping Zhang,Zhi-Cheng Tao,Xian Zhang,Zhen-Xing Yang
An NADH-dependent reductase (SsCR) was discovered by genome data mining. After SsCR was overexpressed in E. coli BL21, recombinant E. coli CCZU-A13 with high reductase activity and excellent stereoselectivity for the reduction of ethyl 4-chloro-3-oxobutanoate (COBE) into ethyl ( R )-4-chloro-3-hydroxybutanoate (( R )-CHBE) was screened using one high-throughput colorimetric screening strategy. After the reaction optimization, a highly stereoselective bioreduction of COBE into ( R )-CHBE (>99% ee ) with the resting cells of E. coli CCZU-A13 was successfully demonstrated in n -butyl acetate–water (10:90, v/v) biphasic system. Biotransformation of 600 mM COBE for 8 h in the biphasic system, ( R )-CHBE (>99% ee ) could be obtained in the high yield of 100%. Moreover, the broad substrate specificity in the reduction of aliphatic and aromatic carbonyl compounds was also found. Significantly, E. coli CCZU-A13 shows high potential in the industrial production of ( R )-CHBE (>99% ee ) and its derivatives.
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MICROCHIMICA ACTA
·
2016
·Vol. 183
·期 6
·1831-1837
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IF 4.831
Yang Zhen,Liu Yan,Lei Chong,Sun Xue-cheng,Zhou Yong
We report on a method for ultrasensitive detection and quantification of the pathogen Escherichia coli ( E. coli ), type O157:H7. It is using a tortuous-shaped giant magnetoimpedance (GMI) sensor in combination with an open-surface microfluidic system coated with a gold film for performing the sandwich immunobinding on its surface. Streptavidin-coated super magnetic Dynabeads were loaded with biotinylated polyclonal antibody to capture E. coli O157:H7. The E. coli -loaded Dynabeads are then injected into the microfluidics system where it comes into contact with the surface of gold nanofilm carrying the monoclonal antibody to form the immunocomplex. As a result, the GMI ratio is strongly reduced at high frequencies if E. coli O157:H7 is present. The sensor has a linear response in the 50 to 500 cfu·mL −1 concentration range, and the detection limit is 50 cfu·mL −1 at a working frequency of 2.2 MHz. In our perception, this method provides a valuable tool for developing GMI-based microfluidic sensors systems for ultrasensitive and quantitative analysis of pathogenic bacteria. The method may also be extended to other sensing applications by employing respective immunoreagents.
Fig. (a) Graphical illustration of the test setup. (b) Relationship of GMI signal vs. E. coli O157:H7 concentration.
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INSECT MOLECULAR BIOLOGY
·
2017
·Vol. 26
·期 5
·642-653
·
IF 2.844
J. Luo,Z. Li,C. Ma,Z. Zhang,J. J. Hull,C. Lei,S. Jin,L. Chen
Insect sex pheromones (SPs) are central to mate-finding behaviour, and play an essential role in the survival and reproduction of organisms. Understanding the roles, biosynthetic pathways and evolution of insect chemical communication systems has been an exciting challenge for biologists. Compared with Lepidoptera, little is known about the mechanisms underlying pheromone biosynthesis in Hemiptera. In this study, we isolated and characterized two new desaturase-like genes, termed Asutdes1 and Asutdes2 , from Adelphocoris suturalis , an important agricultural pest in China. Although the two genes encode an identical protein, Southern blot analysis revealed that they are duplicated genes. The Asutdes2 transcript is more abundant than Asutdes1 in the tissues tested, in particular the metathoracic scent gland and fat body. Silencing Asutdes expression in females by injecting double-stranded RNA ( dsAsutdes ) against a portion of the coding sequence shared by the two genes enhanced the production of (E)-4-oxo-2-hexenal, a component of the A. suturalis SP blend, and dramatically suppressed the sexual attractiveness of A. suturalis females. We conclude that dsAsutdes is associated with the SP biosynthetic pathway in A. suturalis .
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Innovative Food Science & Emerging Technologies
·
2024
·
IF 6.3
Qian Lin,Wei Liang,Mengting Yan,Wenqing Zhao,Wenhao Li
To improve the processing suitability of sorghum flour for staple food applications of sorghum, this study uses E -beam irradiation (EBI) to pre-treat it to investigate the influence of EBI dose on the structure and properties of the mixed flour-dough-bread. The results showed that EBI enhanced mixed flour solubility by improving the components' structure or functionality of sorghum flour, reducing its swelling power, viscosity, and thermal properties. The fermentation properties of the mixed dough reached optimal at 4 kGy, and were almost identical to those of wheat dough. In addition, the microstructure of the mixed flour dough was optimized at 4 kGy, and the textural properties of its bread were also improved. Low-dose EBI can improve the processing suitability of sorghum flour and reduce the destruction of the gluten network of mixed doughs by miscellaneous grains , which is a promising application for processing other cereal-based products.
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Frontiers in Pharmacology
·
2024
·
IF 4.4
Zhang Jinju,Lin Danlin,Wu Yuting,Chen Lixia,Ma Zhiguo,Wu Menghua,Liu Xindan,Zhang Ying,Cao Hui
Background: Equus asinus L. [Equidae; Asini Corri Colla] (donkey-hide gelatin, E-Jiao) is a traditional Chinese medicine renowned for its exceptional blood-supplementing effect. However, the specific components that contribute to its efficacy remain elusive. This study aimed to demonstrate that peptides are responsible for E-Jiao’s blood-supplementing effect and to explore the specific peptides contributing to its efficacy.Methods: The low molecular weight peptides of E-Jiao (LMEJ) were obtained using an in vitro digestion method. LMEJ and peptides in the rat bloodstream were characterized by peptidomics analysis. The blood-supplementing effect of LMEJ was assessed using blood-deficient zebrafish and mouse models. The effect of the peptides detected in rat blood was evaluated using the same zebrafish model, and network pharmacology analysis was performed to investigate the underlying mechanisms.Results: A total of 660 unique peptides were identified within LMEJ. Both E-Jiao and LMEJ significantly alleviated myelosuppression in mice but only LMEJ attenuated myelosuppression in zebrafish. After the administration of E-Jiao to rats, 67 E-Jiao-derived peptides were detected in the bloodstream, 41 of which were identical to those identified in LMEJ. Out of these 41 peptides, five were synthesized. Subsequent verification of their effects revealed that two of them were able to alleviate myelosuppression in zebrafish. Network pharmacology study suggested that E-Jiao may exert a blood-supplementing effect by regulating signaling pathways such as JAK-STAT, IL-17 and others. These results indicated that peptides are at least partially responsible for E-Jiao’s efficacy.Conclusion: This study provides a crucial foundation for further exploration of the bioactive components of E-Jiao.
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BIORESOURCE TECHNOLOGY
·
2016
·
IF 4.917
Yu-Cai He,Zhi-Cheng Tao,Jun-Hua Di,Liang Chen,Lin-Bing Zhang,Dan-Ping Zhang,Gang-Gang Chong,Feng Liu,Yun Ding,Chun-Xia Jiang,Cui-Luan Ma
It was the first report that the concentrated hydrolyzates from the enzymatic hydrolysis of dilute NaOH (3 wt%)-soaking rice straw at 30 °C was used to form [Bmim]PF 6 -hydrolyzate (50:50, v/v) media for bioconverting ethyl 4-chloro-3-oxobutanoate (COBE) into ethyl ( R )-4-chloro-3-hydroxybutanoate [( R )-CHBE] (>99% e.e. ) with recombinant E. coli CCZU-A13. Compared with pure glucose, the hydrolyzates could promote both initial reaction rate and the intracellular NADH content. Furthermore, emulsifier OP-10 (20 mM) was employed to improve the reductase activity. Moreover, Hp-β-cyclodextrin (0.01 mol Hp-β-cyclodextrin/mol COBE) was also added into this bioreaction system for enhancing the biosynthesis of ( R )-CHBE from COBE by E. coli CCZU-A13 whole-cells. The yield of ( R )-CHBE (>99% e.e. ) from 800 mM COBE was obtained at 100% in the [Bmim]PF 6 -hydrolyzate (50:50, v/v) media by supplementation of OP-10 (20 mM) and Hp-β-CD (8 mM). In conclusion, an effective strategy for the biosynthesis of ( R )-CHBE was successfully demonstrated.
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Food & Function
·
2018
·Vol. 9
·期 10
·5103-5114
·
IF 3.289
Zongyuan Wu,Zumeng Xia,Bangfu Wu,Fang Wei,Xin Lv,Ya Xie,Shuling Xu,Jiqu Xu,Hong Chen,Fenghong Huang
Atherosclerosis is a chronic disease associated with oxidative stress and inflammatory activation and is the main underlying trigger for cardiovascular disease. There are many cardiovascular health products in the market; in order to evaluate the effect of these products, in this paper, a novel lipid profiling platform was established using the shot-gun mass spectrum method for the analysis of free fatty acid and phospholipids, and the high performance liquid chromatography coupled with mass spectroscopy method for the analysis of lipid mediators and triacylglycerol, respectively, in serum from male apolipoprotein E-knock out mice after different diet interventions. Changes in the four lipids above, and pathways and regulation of lipid metabolism in mice from different groups were further investigated. The result showed that all cardiovascular health products showed some certain potential to alleviate atherogenesis and ameliorate inflammation; among them, lemon apple cider vinegar drink and seal oil could significantly decrease triacylglycerol in mouse serum. The establishment of this lipidome profiling platform helps to better understand the metabolism regulation and intervention mechanism of different cardiovascular health products in chronic diseases, such as atherogenesis. This platform could be applied to other cardiovascular health products and provide reliable lipid profiling data for their potential effect.
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FlatChem
·
2026
·
IF 5.7
Jian Yang,Zhixiang Liu,Xiang Liu
We report a green approach of preparing cationic surfactant with starch. • None of any organic solvent is used during the whole procedure. • The cationic surfactant differs entirely with conventional one in structure. Renewable carbohydrates provided abundant resources for the preparation of carbon dots (CDs), which could serve as a starting material for synthesis of fine chemicals. In this work, soluble starch was first formulated into an aqueous dispersion and then converted into starch-derived CDs (designated as SCDs) via hydrothermal treatment. Subsequently, SCDs were facilely and efficiently modified with n -dodecylamine in an aqueous medium (the modified product was denoted as SCDs-C12). Experimental results demonstrated that a dilute solution of SCDs-C12 reduced the surface tension of water from 72.0 to 31.9 mN·m −1 (25 °C), and its surface activity was comparable to that of some novel gemini surfactants. Additionally, the positively charged SCDs-C12 exhibited excellent performance in inhibiting the growth of E. coli , with a minimum inhibitory concentration of only 0.05 mg·mL −1 , outperforming many previously reported CD-based materials. In summary, this work developed an environmentally friendly and green strategy for the fabrication of multifunctional cationic surfactants. Download: Download high-res image (74KB) Download: Download full-size image
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FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Na Wang,Xiangyang Zhang,Li Xue,Zhen Zhang,Li Fu,Xuejiao Ren,Hui Zhang,Bo Zhang,Xiaoyu Jiang,Lili Zhang,Mei Wang
Daoguang nianwu (DGNW), a representative Chinese Chenxiang-type baijiu stored in wooden Jiuhai, features the distinctive “three aroma-types in one sip” profile. Six vintages (0, 1, 3, 5, 10 and 20 years) were analyzed via GC-E-Nose, GC-IMS, GC-MS-O, ROAV and chemometrics to characterize its aging aroma. A total of 130 aroma compounds were identified, with esters dominant at 43.08%. Young DGNW (0Y) exhibited more numerous key aroma-active components including ethyl 2-methylbutyrate, furfural, 4-ethylguaiacol and dimethyl trisulfide (DMTS), presenting “fruity”, “floral”, and “caramel” notes alongside “herbal, smoky” and “pickle” off-odors. In contrast, aged DGNW (5Y) displayed richer diversity with enhanced “sour”, “woody, fresh” notes attributed to hexanoic acid, ethyl octanoate, phenylethyl alcohol and heptanol, with complete elimination of off-odors. OPLS-DA revealed three aging stages: young (0Y), low-aged (1Y–3Y) and high-aged (5Y–20Y), identifying 5 years as the critical turning point with stabilization after 10 years. Cluster analysis (CA) validated 12 dual-functional key aging markers. Notably, most of 51 screened markers decreased during storage, including furfural—a pattern contrary to pottery-jar-aged baijiu. Based on these findings, we propose an “initial alkalinity to late acidity” hypothesis for the wooden Jiuhai aging environment, providing theoretical insights for optimizing wooden Jiuhai storage technology in Chinese baijiu production.
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危险品化学品经营许可证（带存储）