MEDICAL SCIENCE MONITOR
·
2018
·
IF 1.894
Zeng Li,Xiao-chang Liu,Rui Li,Jun Chang
Background Beta-secretase 1 (BACE1) is a rate-limiting enzyme in the generation of amyloid beta peptides, which are associated with Alzheimer’s disease (AD). It has been reported that Schisandrin B could improve cognitive functions in animal models of AD, but the underlying mechanisms are not completely understood. Material/Methods In this research, in order to investigate the effects of Schisandrin B on amyloid-β (Aβ) metabolism and its mechanisms, amyloid precursor protein (APP) and its proteolytic products were determined by enzyme-linked immunosorbent assay (ELISA), western blotting, and RT-PCR after incubation of N2a/Swe cells with Schisandrin B. Results The results indicated that Schisandrin B can significantly reduce the level of secretion of Aβ 40 and Aβ 42 secreted in N2a/Swe cells. Additionally, there was nonsignificant change in APP level after Schisandrin B treatment. Treatment of Schisandrin B dramatically reduced the mRNA and protein expression levels of BACE1. Moreover, Schisandrin B treatment resulted in a reduction of protein level of sAPPβ, an APP fragment cleavage by BACE1. Conclusions These results suggest that Schisandrin B inhibits the transcription and translation of BACE1, suppresses the activity of BACE1, and ultimately attenuates Aβ generation, which provides a novel mechanism for the regulation of Aβ metabolism by Schisandrin B.
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PubMed
高影响因子
Toxins
·
2024
·Vol. 16
·期 11
·494
·
IF 3.9
Chenying Yang,Li Ding,Qiyi He,Xiya Chen,Haiting Zhu,Feng Chen,Wanzhou Yang,Yuexin Pan,Zhiyuan Tai,Wenhao Zhang,Zeyuan Yu,Zening Chen,Xiaodong Yu
Kraits are venomous snakes of the genusBungarusfrom the familyElapidae. Their venom typically demonstrates neurotoxicity; however, the toxicity is significantly influenced by the snake’s species and geographical origin. Among theBungarusspecies,Bungarus suzhenaeandB. bungaroideshave been poorly studied, with little to no information available regarding their venom composition. In this study, a proteomic approach was employed using LC-MS/MS to identify proteins from trypsin-digested peptides. The analysis revealed 102 venom-related proteins from 18 distinct functional protein families in the venom ofB. suzhenae, with the primary components being three-finger toxins (3-FTx, 25.84%), phospholipase A2(PLA2, 40.29%), L-amino acid oxidase (LAAO, 10.33%), Kunitz-type serine protease inhibitors (KUN, 9.48%), and snake venom metalloproteinases (SVMPs, 6.13%). In the venom ofB. bungaroides, 99 proteins from 17 families were identified, with primary components being 3-FTx (33.87%), PLA2(37.91%), LAAO (4.21%), and KUN (16.60%). Enzymatic activity assays confirmed the presence of key venom enzymes. Additionally, the LD50 values forB. suzhenaeandB. bungaroideswere 0.0133 μg/g and 0.752 μg/g, respectively, providing a reference for toxicity studies of these two species. This research elucidates the proteomic differences in the venoms of these two species, offering a foundation for developing antivenoms and clinical treatments for envenomation.
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高影响因子
DALTON TRANSACTIONS
·
2021
·Vol. 50
·期 42
·15413-15421
·
IF 4.39
Jingwei Liu,Yanjie Liang,Shao Yan,Dongxun Chen,Shihai Miao,Weili Wang
Luminescent materials that emit in the narrowband ultraviolet-B (NB-UVB; 310–313 nm) spectral region have attracted considerable attention due to their unique spectral features, which endow them with great potential applications in the fields of photochemistry and photomedicine. However, NB-UVB persistent luminescent materials are relatively lacking, especially materials that are excitable by natural sunlight. Here we report the NB-UVB persistent luminescence of Gd3+ in (Y,Gd)3Ga5O12:Bi3+ garnets by making use of the persistent energy transfer from Bi3+ to Gd3+. The optimal Bi3+ and Gd3+ concentrations for the maximum energy transfer efficiency are determined and persistent NB-UVB light emission with a peak wavelength at 313 nm and an afterglow time of more than 24 h is successfully achieved in the (Y,Gd)3Ga5O12:Bi3+ phosphor. More importantly, the as-synthesized NB-UVB persistent phosphors are also excitable by the widely available natural sunlight and exhibit exceptional NB-UVB persistent luminescence performance. Benefitting from the visible-blind emission feature, interference-free capability from indoor ambient light and self-sustained optical characteristic, the developed sunlight-excitable NB-UVB persistent phosphors here not only hold great promise for covert optical tagging applications, but also open new opportunities for optical data storage in a bright indoor environment.
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高影响因子
JOURNAL OF MEDICAL VIROLOGY
·
2025
·Vol. 97
·期 5
·e70395
·
IF 4.6
Qiuhong You,Kaifeng Wang,Zhou Zhao,Heqi Zhou,Zhixian Lan,Hongyan Liang,Rui Deng,Wanying Li,Sheng Shen,Routing Wang,Kaikai Zhang,Dekai Zheng,Jian Sun
In chronic hepatitis B (CHB) patients under antiviral treatment, liver injury, as evidenced by elevated alanine transaminase (ALT), is associated with unfavorable outcomes and needs effective treatment. The interaction between gut microbiota and liver injury in CHB patients remains unclear. Using a case-control design, 28 cases with elevated ALT and 28 matched controls with normal ALT were randomly selected from CHB patients with viral control. Clinical characteristics were comparable between groups. Metagenomic sequencing revealed that Bacteroides fragilis was decreased in cases and exhibited the greatest disparity between cases and controls. Mice colonized by gut microbiota from cases exhibited more severe liver damage in both LPS-induced and MCD diet-induced liver injury models, and had a lower abundance of B. fragilis compared to mice colonized by gut microbiota from controls. Oral gavage of B. fragilis improved both LPS-induced and MCD diet-induced liver injury. Metabolomics analysis revealed that the levels of 7-Ketolithocholic acid (7-Keto-LCA) were positively correlated with B. fragilis and significantly increased in the cultural supernatant of B. fragilis . Consistently, 7-Keto-LCA exerted protective effects against both LPS-induced and MCD diet-induced liver damage. Targeting gut microbiota might be a promising therapeutic treatment for alleviation residual liver inflammation in CHB patients with viral control.
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Hongrui Zhang,Ya-nan Zhang,Like Li,Xuegang Li,Yong Zhao
A method for the determination of cholesterol using rhodamine derivatives as chromogenic substrates was proposed in this study. The oxidation of rhodamine B hydrazide (RBH) by hydrogen peroxide (H2O2) was catalyzed by a peroxide-mimicking enzyme to produce the fluorescent product. The reaction was further combined with the oxidase system to achieve indirect determination of cholesterol concentration, which can be catalyzed by cholesterol oxidase to produce H2O2 as an intermediate product. Optical fiber optofluidic lasers (FOFLs), as a sensitive biochemical detection platform, have the ability to distinguish small differences in the amount of fluorescence products generated by the reaction and were used to achieve sensitive cholesterol detection. Under optimized experimental conditions, the dynamic range of three orders of magnitude was obtained in the determination of H2O2 with a lower limit of detection (LOD) of 1.63 μM, and the assay time was as short as 3.5 min. On this basis, the designed cholesterol sensor obtained a linear range of 2.55–652.67 μM with a lower LOD of 2.55 μM and proved to have good selectivity. The possibility of cholesterol determination in human serum was initially tested. The sensor was also characterized by easy signal collection, wash-free procedure, controlled sample consumption, fast assay, low cost, and simple operation.
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PubMed
高影响因子
MOLECULES
·
2025
·Vol. 30
·期 8
·1717
·
IF 4.6
Chenghong Ao,Mai Shan,Yue Zhang,Xiang Li,Ying Kong,Xinwei Dong,Yang Liu,Danping Wu
Biochar has attracted considerable interest owing to its high adsorption capacity; however, the mechanisms through which environmental factors influence the release of adsorbed pollutants remain unclear. This study investigates the adsorption and desorption dynamics of Rhodamine B (RhB) on biochars B2 and B6, which were pyrolyzed at temperatures of 200 °C and 600 °C, respectively, under varying conditions. The results indicated that there was no significant difference in removal efficiency at low RhB concentrations; however, at a concentration of 600 mg/L, biochar B2 had a higher removal efficiency than B6, likely attributable to more adsorption sites. Increased temperatures were found to enhance desorption from both B2 and B6, with B6 exhibiting a faster desorption rate. This phenomenon may be due to the stronger hydrogen bonding between B2 and RhB, which could inhibit desorption. In addition, elevated pH values facilitated desorption, presumably through electrostatic repulsion. Under alkaline conditions, B2 released a greater amount of dissolved organic matter (DOM), leading to increased RhB desorption relative to B6. This study offers a valuable reference for evaluating the environmental risk associated with the application of biochar in real-world settings.
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高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Yaxin Yu,Fei Xia,Qi Cao,Hanyuan Zhang,Wanting Shi,Peng Zhang,Wenxiu Sun
The adoption of innovative active food packaging has become increasingly crucial for suppressing microbial proliferation and prolonging the shelf life of food items. In this research, we detail the development of novel photodynamic antimicrobial nanofiber films via the electrospinning method, employing polyvinyl alcohol (PVA) as the base material and erythrosine B (EB) as the photosensitizing agent. We examined the chemical composition, photophysical characteristics, and antibacterial properties of these films. FTIR spectroscopy validated the integration of EB within the nanofiber structures. Furthermore, EB enhanced the films' resistance to water, lowered water vapor permeability, and displayed moderate mechanical strength. The nanofiber films exhibited a remarkable capability for generating reactive oxygen species and showed significant antibacterial effectiveness against E. coli , S. aureus , P. putida , and P. aeruginosa , maintaining an overall migration limit of EB within the acceptable threshold of 10 mg/dm 2 . We utilized PVA/EB-G films along with tray packaging to preserve chilled horsemeat, assessing physicochemical alterations and microbial populations. The storage life of chilled horsemeat was prolonged by 6 days in comparison to controls that did not include PVA/EB-G films. The results of this investigation propose a novel strategy for harnessing photodynamic mechanisms in the preservation of chilled meat.
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PubMed
高影响因子
Molecular Therapy-Nucleic Acids
·
2021
·
IF 8.886
Jing Miao,Xiqin Yang,Xuwei Shang,Zhe Gao,Qian Li,Yun Hong,Jiaying Wu,Tingting Meng,Hong Yuan,Fuqiang Hu
Hepatitis B (HB) is a viral infectious disease that seriously endangers human health, and since there are no radical drugs to counter this, effective and safe therapies urgently need to be developed. HB virus (HBV) mainly infects hepatocytes (HCs), while the drugs are easily phagocytosed by Kupffer cells (KCs). In this study, the glutathione concentration difference between HCs and KCs was examined and utilized in an ideal drug-release strategy. Here, galactosylated chitosan-oligosaccharide-SS-octadecylamine (Gal-CSSO) was prepared to accurately deliver 10-23 DNAzyme DrzBC (blocking HBeAg expression) or DrzBS (blocking HBsAg expression) in targeted HB therapy. In vitro Gal-CSSO systems exhibited low cytotoxicity, endosomal escape, and glutathione responsiveness. The HBeAg and HBsAg secretion of HepG2.2.15 was significantly decreased by Gal-CSSO systems, and the maximum inhibition rates were 1.82-fold and 2.38-fold greater than those of commercial Lipofectamine 2000 (Lipo2000) systems. In vivo Gal-CSSO systems exhibited HC targeting and HC microenvironmental responsiveness without noticeable hepatotoxicity or systemic toxicity. The HBeAg and HBsAg titers of the HBV-infected mice were evidently decreased by Gal-CSSO systems, and the inhibition rates were 1.52-fold and 1.22-fold greater than those of Lipo2000 systems. This study presents a kind of glycolipid-like polymer micelles that promise efficient and safe gene therapy of HB.
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高影响因子
Journal of Materials Chemistry A
·
2015
·Vol. 3
·期 46
·23352-23359
·
IF 7.443
Wuxue Zhao,Sheng Han,Xiaodong Zhuang,Fan Zhang,Yiyong Mai,Xinliang Feng
A new series of B, N-containing cross-linked polymers (PPs-BN) were achieved via Sonogashira cross coupling. These polymers exhibit very high carbon yields of around 70–80% even at 800 °C, which allow them to be efficiently converted to B/N co-doped porous carbons after pyrolysis at high temperature under an inert atmosphere. The materials have been fully characterized by Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analyses, nitrogen sorption measurements and X-ray photoelectron spectroscopy, revealing their high contents of boron and nitrogen up to ∼3.21% and ∼5.72%, respectively, as well as porous structures with the largest specific surface area of 291 m2 g−1. Their CO2 capacities reached 3.25 mmol g−1 at 273 K under 1 atm. In addition, the very high selectivity for CO2/CH4 with a ratio of more than 5 : 1 at 298 K renders them applicable for gas separation and purification.
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高影响因子
JOURNAL OF THE EUROPEAN CERAMIC SOCIETY
·
2022
·
IF 6.364
Wanli Zhao,HongLi Suo,Shuo Wang,Lin Ma,Lei Wang,Qiuliang Wang,Zili Zhang
MgB 2 superconductor pellets were synthesized through Mg gas infiltration method using nanosized- and microsized B powders. There was a marked difference in the superconducting properties of the two samples, particularly in the pinning force and dominant pinning mechanism. The microstructures of the samples were observed using HR-TEM and STEM-HAADF, and the results showed that the primary reason for the difference in the superconducting properties is the distribution of the nanosized second-phase particle MgO. Additionally, a feasible reaction model for the Mg gas infiltration method was established. Compared to the Mg liquid infiltration method, the gas infiltration showed better penetrability ability with a small amount of residual Mg. This study presents a novel synthesis process to fabricate an MgB 2 pellet with superior density and superconducting properties. This method can be used in multiple applications such as superconducting bearings, compact superconductor magnets, and magnetic shielding.
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高影响因子
COLLOID AND POLYMER SCIENCE
·
2014
·Vol. 292
·期 9
·2071-2082
·
IF 2.41
Fu Yutong,Xia Xinyu,Zhang Yan,Ye Jinhai,Lang Meidong
Functional star-shaped 4-arm poly(ethylene glycol)- b -poly[( ε -caprolactone- co - γ -amino- ε -caprolactone)] (4-arm PEG- b -P(CL- co -ACL) was synthesized through ring-opening polymerization. The structure of the copolymer was confirmed by 1 H NMR, Fourier transform infrared spectroscopy (FTIR), and gel permeation chromatography (GPC). To further understand the copolymers, the difference of the conversion rate between ε -caprolactone (CL) and γ -(carbamic acid benzyl ester)- ε -caprolactone (CABCL) and the detailed deprotection condition were studied. The thermal property of the copolymer was analyzed by WAXR and differential scanning calorimetry (DSC), which demonstrated that the thermal property could be well adjusted. The pH-responsive behavior of the copolymers was studied in detail by dynamic light scattering (DLS), pH titration, and pyrene fluorescence methods, which indicated that it could form micelles and exhibit pH responsibility. Moreover, the copolymer was nontoxic and had good biocompatibility according to the results by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay.
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高影响因子
SURFACE & COATINGS TECHNOLOGY
·
2015
·
IF 1.998
Wang Peng,Zhou Shanbao,Zhang Xinghong,Gui Kaixuan,Li Yongxia,An Jiadong,Han Wenbo
To protect graphite matrix from oxidation , B modified ZrB 2 –SiC coatings were prepared on SiC coated graphite by a two-step pack cementation . The microstructure, thermal cycling and oxidation resistant properties of the coatings were studied. The results show that the as-prepared coatings were mainly composed of SiC, ZrB 2 and Si. ZS50-1B, ZS50-3B and ZS50-5B showed good thermal cycling resistance with 97.4%, 98.2% and 98.6% weight retention after 15 thermal cycles between 1500 °C and room temperature. ZS50-7B and ZS50-9B showed good oxidation resistance with 93.5% and 97.5% weight retention after 19 h oxidation at 1500 °C.
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高影响因子
危险品化学品经营许可证（带存储）