ACS Nano
·
2022
·
IF 18.027
Dandan Wang,Yong Wang,Haijuan Li,Yanchao Han,Ping Hu,Kongshuo Ma,Mordechai Sheves,Yongdong Jin
Nanofluidics is an emerging hot field that explores the unusual behaviors of ions/molecules transporting through nanoscale channels, which possesses a broad application prospect. However, in situ probing bioactivity of functional proteins on a single-molecule level by a nanofluidic device has not been reported, and it is still a big challenge in the field. Herein, we reported a biological nanofluidic device with a single-protein sensitivity, based on natural proton-pumping protein, bacteriorhodopsin (bR), and a single SiNx nanopore. Nanofluidic single-molecule probing of bR proton-pumping activity and its light response were achieved under applied voltage of 0 V, by biologically self-powered steady-state ionic current nanopore sensing. Green-light irradiation of the device led to the monitoring of a steady-state proton current of ∼3.51 pA/per bR trimer, corresponding to charge density of 815 μC/cm2 generated by each bR monomer, which far exceeded the previously reported value of 1.4 μC/cm2. This finding and method would promote the development of artificial biological and hybrid nanofluidic devices in biosensing and energy conversion applications.
研究领域:
纳米流体学
·
生物物理学
·
生物传感
·
能量转换
✓ 已验证
DOI
高影响因子
ELECTROPHORESIS
·
2019
·
IF 2.754
Qian Yang,Bin Su,Yafeng Wang,Wanhao Wu
In this work, an efficient electroosmotic pump (EOP) based on the ultrathin silica nanoporous membrane (u-SNM), which can drive the motion of fluid under the operating voltage as low as 0.2 V, has been fabricated. Thanks to the ultrathin thickness of u-SNM (∼75 nm), the effective electric field strength across u-SNM could be as high as 8.27 × 10 5 V m −1 in 0.4 M KCl when 1.0 V of voltage was applied. The maximum normalized electroosmotic flow (EOF) rate was as high as 172.90 mL/min/cm 2 /V, which was larger than most of other nanoporous membrane based EOPs. In addition to the ultrathin thickness, the high porosity of this membrane (with a pore density of 4 × 10 12 cm −2 , corresponding to a porosity of 16.7%) also contribute to such a high EOF rate. Moreover, the EOF rate was found to be proportional to both the applied voltage and the electrolyte concentration. Because of small electrokinetic radius of u-SNM arising from its ultrasmall pore size (ca. 2.3 nm in diameter), the EOF rate increased with increasing the electrolyte concentration and reached the maximum at a concentration of 0.4 M. This dependence was rationalized by the variations of both zeta potential and electrokinetic radius with the electrolyte concentration.
研究领域:
材料科学
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纳米技术
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Yang Yang,Wei Ma,Baiyang Chen,Chong Peng,Wang Luo,Huan He
Haloacetic acids (HAAs) are a group of disinfection byproducts ubiquitously found in disinfected drinking water and they are currently regulated by some governments. Conventional HAAs analytic methods employ either gas chromatography (GC), two-dimensional ion chromatography (2D-IC), or ion chromatography with electrospray ionization tandem mass spectrometry (IC-ESI-MS/MS) to reduce the effect of interfering compounds and enhance detection resolution; however, they are either laborious in sample preparation procedures or too expensive to be used by common laboratories. To bypass these problems, this study proposes to analyze HAAs by employing a one-pump column-switching ion chromatography (OPCS-IC) that allows direct sample injection without requiring extraction and derivatization pretreatments. Unlike 2D-IC method, which uses two pumps and two parallel sets of IC columns, this method applies only one pump and one column. The tailored OPCS-IC removes coexisting anions from water and enriches HAAs simultaneously, thereby distinguishing HAAs from interfering anions and magnifying the signals of HAAs. For example, when HAAs were enriched by five times, the coexisting F − , Cl − , Br − , NO 3 – , and SO 4 2− were removed by 62.4%, 93.9%, 99.7%, 99.9%, and 98.9%, respectively. Meanwhile, the recoveries of HAAs dosed into real samples ranged from 84% to 102%, similar to those obtained from conventional GC method, meaning that the convenience of this method did not compromise its performance. The study hence proves an easy-to-use and relatively cheaper method for measuring HAAs in drinking water.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
·
2023
·Vol. 396
·期 8
·1759-1771
·
IF 3.6
Liu Fei,Xu Yanjun,Wang Li,Ma Xifeng,Zhang Zhen,Zhuang Xiaomei
Anaprazole, a new proton pump inhibitor (PPI), is designed for the treatment of acid-related diseases, such as gastric ulcers and gastroesophageal reflux. This study explored the in vitro metabolic transformation of anaprazole. The metabolic stabilities of anaprazole in human plasma and human liver microsomes (HLM) were analyzed by liquid chromatography-tandem mass spectrometry (LC–MS/MS). Then, the contribution (%) of non-enzymatic and cytochrome P450s (CYPs) enzyme-mediated anaprazole metabolism was assessed. To obtain the metabolic pathways of anaprazole, the metabolites generated in HLM, thermal deactivated HLM, and cDNA-expressed recombinant CYPs incubation systems were identified by ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC/Q-TOF–MS). Results showed that anaprazole was very stable in human plasma and unstable in HLM. The contribution (%) of non-enzymatic vs . CYPs enzyme-mediated metabolism was 49% vs . 51%. CYP3A4 was the major enzyme (48.3%), followed by CYP2C9 (17.7%) and CYP2C8 (12.3%), in responsible for the metabolism of anaprazole. Specific chemical inhibitors targeting CYP enzymes notably blocked the metabolic transformation of anaprazole. Six metabolites of anaprazole were identified in the non-enzymatic system, whereas 17 metabolites were generated in HLM. The biotransformation reactions mainly included sulfoxide reduction to thioether, sulfoxide oxidation to sulfone, deoxidation, dehydrogenation, O-dealkylation or O-demethylation of thioether, O-demethylation and dehydrogenation of thioether, O-dealkylation and dehydrogenation of thioether, thioether O-dealkylation and dehydrogenation of thioether, and O-dealkylation of sulfone. Both enzymatic and non-enzymatic metabolisms contribute to the clearance of anaprazole in human. Anaprazole is less likely to develop drug-drug interactions in clinical use compared to other PPIs.
研究领域:
药理学
·
药物代谢
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2023
·
IF 4.2
Suzhou Dai,Yonggao Yin,Yikai Wang,Xuanlin Liu,Maurizio Peruzzini,Francesco Barzagli
Chemical heat pump based on ammonium carbamate (AC) is a promising low-grade heat utilization technology. It is essential to know the chemical equilibrium properties in the application of AC-based technologies. AC is usually mixed with solvents to realize circulation. However, the existing studies mainly focus on AC in the solid phase. Therefore, the influence of solvents on the chemical equilibrium of AC was experimentally investigated in this work. The results confirm the solvent effect on the equilibrium pressure of AC in the range of 10∼90 °C. For solvents capable of dissolving AC, the equilibrium pressure increases with both AC concentration and temperature. The solvent effect is mainly due to the influence on both the reaction enthalpy and entropy change terms. An equilibrium pressure–temperature–concentration relationship of AC solution is established. These results provide essential parameters for the design and operation of low-grade heat management and utilization systems based on AC.
研究领域:
热力学
·
化学工程
·
能源技术
✓ 已验证
产品关联
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2025
·
IF 7.3
Mengdi Xu,Hermela Ejegu Feysa,Xiaoqing Ni,Guangbiao Xu,Qingshuai Yan,Hua Shen,Yueqi Zhong,Xingjie Wang
Optimized management of wound exudate is pivotal in modulating the wound healing microenvironment and facilitating tissue regeneration. Conventional dressings, however, are constrained by limited absorption capacity and a slow drainage rate, frequently resulting in excessive exudate accumulation that hinders the healing process. In this study, we developed hydrophilic kapok fiber gauze (H-KG) and Janus kapok fiber gauze (J-KG) through a sustainable and scalable hydroentanglement forming process combined with chemical modification, leveraging the unique hollow tubular structure of kapok fibers. H-KG and J-KG achieved rapid absorption, high capacity storage, and efficient evaporation through synergistic wettability modulation and capillary action-enhanced liquid transport within the hollow structure. The kapok fiber-based gauze demonstrated remarkable sustainable liquid self-pumping capabilities, exhibiting a saturation absorption rate of approximately 1346% with complete liquid evaporation achieved within 1 h. In vivo experiments revealed that both H-KG and J-KG significantly mitigated wound exudate accumulation compared with cotton gauze (CG), thereby accelerating wound healing. Quantitative analysis showed that on day 12 post-treatment, the H-KG and J-KG exhibited residual wound areas measuring 48.23% and 34.41%, respectively, of CG. This work elucidated the mechanistic role of hollow fiber in exudate management while proposing an innovative wound dressing with dynamic fluid regulation capabilities.
研究领域:
生物材料
·
生物医学工程
·
组织工程
·
伤口愈合
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流体传输材料
·
纤维科学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2024
·
IF 8.1
Yuge Yan,Xiaofeng Gu,Mingyang Zhou,Zhijie Wang,Xifen Gong,Yu Chen,Qingyu Li,Jiang Liu
Here we use the electrochemical hydrogen pump derived from a protonic ceramic cell (PCC) to realize the first demonstration of separating hydrogen from simulated peanut shell pyrolysis gas (64% H 2 , 26% CO, 4% CO 2 , and 6% CH 4 ). The single PCC is configured with a thick Ni–BaCe 0.7 Zr 0·1 Y 0·2 O 3-δ cathode, a thin BaCe 0·7 Zr 0·1 Y 0·18 Fe 0·02 O 3-δ electrolyte, and an Ag–Ce 0.8 Gd 0.2 O 1.9 anode with an effective area of 1.5 cm 2 . At 650 °C and a feeding current density of −500 mA cm −2 , a hydrogen flux of 2.34 ml min −1 cm −2 , a Faradaic efficiency of 62%, and a hydrogen recovery rate of 29% are achieved. In addition, 20 wt% Ni is mixed into the Ag-GDC electrode to achieve a hydrogen flux of 3.68 ml min −1 cm −2 and a Faradaic efficiency of 97%. Additionally, a 20-h measurement for PCCs with the Ni-mixed electrode shows the hydrogen fluxes and Faradaic efficiencies of above 1.49 ml min −1 cm −2 and 98%, respectively.
研究领域:
能源科学
·
材料科学
·
环境工程
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Zekun Li,Jitao Liu,Mengshuang Chi,Xue Miao,Hanxiao Yang,Weiqi Cui,Aifang Yu,Junyi Zhai
On the road to large-scale industrialization of triboelectric nanogenerators (TENGs), higher output is the constant quest. Here, inspired by the ubiquitous synergies in nature, an ultra-high-output TENG is proposed based on the synergistic effect of internal modification of materials along with the external integration of a charge pumping module. The carefully chosen poly (vinylidene fluoride-co-hexafluoropropylene) (P(VDF-HFP)) film possesses strong polarization and high permittivity, which leads to excellent triboelectric properties, and can be further enhanced by doping BaTiO 3 (BTO) nanoparticles . Meanwhile, the elaborately designed and optimized charge pumping module endows it with the ability to pump affluent charges. As a consequence, a TENG based on 8 µm P(VDF-HFP)/BTO 1 wt% composite film can achieve an ultra-high total transfer charge density (TTCD) of 3.5 mC/m 2 under optimal charge pumping conditions. Compared to the output of the P(VDF-HFP)/BTO-based TENG (0.1 mC/m 2 ) and the output of the P(VDF-HFP)-based TENG under charge pumping (2.6 mC/m 2 ), the output of the P(VDF-HFP)/BTO-based TENG under charge pumping is greater than the sum of the above two devices, which indicates that the proposed synergistic effect for designing an ultra-high-output TENG is remarkable and successful. Moreover, a novel charge transfer pattern based on synergistic effect is proposed, expounding the role inherent modifications of P(VDF-HFP) and external integration of charge pumping modules play. This work presents a concept that is at the forefront of contemporary designs for ultra-high output TENGs, providing insight into the preparation of high-performance TENGs in a state-of-the-art manner.
研究领域:
能源材料科学
·
纳米技术
·
物理学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2024
·
IF 5.6
Mimi Sun,Chongbo Ma,Mohammed Y. Emran,Ahmed Kotb,Jing Bai,Ming Zhou
The advanced devices that function fully without the need for external accessories are regarded as a pinnacle goal in the design and construction of modern ones. Staphylococcus aureus ( S. aureus ), a prominent human pathogen, is responsible for causing a wide variety of infections and chronic diseases. Herein, we present the first instance of a fully integrated wireless microfluidic immunosensing system (FIWMIS) capable of conducting point-of-care S. aureus monitoring in real samples of S. aureus -spiked commercial purified drinking water and S. aureus -spiked watermelon juice. The development of the proposed FIWMIS became a reality by conquering significant engineering hurdles in seamlessly integrating a microfluidic unit for liquid sample transport without the need of an external pump, an immunosensing unit for S. aureus monitoring, and an electronic control unit for signal conversion and wireless transmission. Such full integration culminated in a FIWMIS that upholds its pump-free, wireless, and low-cost characteristics for portable monitoring of S. aureus .
研究领域:
生物医学工程
·
微生物学
·
传感器技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENT INTERNATIONAL
·
2019
·
IF 7.943
Qing-Lin Chen,Dong Zhu,Xin-Li An,Jing Ding,Yong-Guan Zhu,Li Cui
Growing evidences have demonstrated that heavy metal contamination can promote the proliferation of antibiotic resistance genes (ARGs) via co-selection. However, effects of nano-metal-materials on the occurrence and level of ARGs in the soil and plant, have not been fully explored. To gain insights into this impact, we conducted a pot experiment by adding nano‑silver particles (AgNPs) as a stimuli and Ag ion (AgNO 3 ) and tetracycline as a comparison. By using high throughput quantitative PCR, our results indicated that application of AgNPs (~20 nm and ~50 nm) at a concentration of 100 ppm resulted in no significant changes in the abundance of ARGs in either soil or phyllosphere ( P > 0.05). Nevertheless, the overall pattern of resistome, especially in soil, was shifted following AgNPs application, with a significance increase in the relative abundance of efflux pumps genes, which is an important mechanism for co-selection of ARGs by heavy metals. By comparison, Ag ion at an equivalent Ag mass of AgNPs markedly increased ARGs abundance and shifted ARGs profile in soil, indicating that free Ag ion had a stronger impact on ARGs than AgNPs. These findings provide new insights in assessing the risks of manufactured nanomaterials accumulated in the environment.
研究领域:
环境科学
·
微生物学
·
土壤学
·
植物科学
✓ 已验证
DOI
PubMed
高影响因子
IEEE SENSORS JOURNAL
·
2024
·
IF 4.3
Xuanxu Nan,Lingyi Xu,Li Yang,Yue Cui
The development of sensors for the detection of albumin concentrations in urine is essential for the long-term detection and treatment of kidney disease. Albumin is an important biomarker for kidney diseases. However, albumin concentrations in patient urine are often too high (more than 0.15 mg/mL), causing competitive lateral flow immunoassays to exceed detection ranges. In this work, we report for the first time a one-step, fluorescent lateral-flow quantification of human urine albumin using an in-situ minipump and clinical evaluation in renal patients, eliminating the need for external sample dilution. The device is constructed by integrating a lateral flow immunoassay strip and an in-situ minipump that sits above the sample/conjugate pad of the strip. The detection is based on competitive immunoassay by using fluorescent nanoparticles as the probe, and the in-situ pump performs on-chip dilution. The linear detection range with this device is 0.1-10 mg/mL for a 1000-fold dilution of the sample with the pump, and 1-100 mg/mL for a 10,000-fold dilution. The device can be used for the direct quantification of albumin in the urine of patients with clinical kidney disease. We anticipate the work can open up exciting new opportunities for the quantification of protein biomarkers, and their wide range of applications in clinical and home-settings.
研究领域:
医疗器械
·
临床诊断
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
Case Studies in Thermal Engineering
·
2022
·
IF 6.268
Ningning Zhang,Mengya Li,Haoyu Zhang,Yue Xiao,Cong Zhang,Shuo Yuan,Min He,Wenyu Yang
To realize the triple functions of “roadway support, thermal insulation and heat regulation” of pumping wet shotcrete (PWS), the microencapsulated phase change material (MPCM) with poly (lauryl methacrylate) as shell and paraffin wax as core was synthetized by interfacial polymerization. The surface morphology, particle size distributions, core-shell compositions, the performances of the thermal stability and phase change of the MPCM were analyzed. The results show that the synthetized MPCM has excellent performances and can be applied in PWS. To obtain the optimal mixed proportions of the key materials (i.e., ceramsite, sand and MPCM) in PWS, the key performance indices were employed in the orthogonal experimental design to test the multiple performances of the PWS containing MPCMs. The results show that these performances are influenced by the dosages of the key materials and the recommended dosages were: 112.5 kg/m 3 of ceramsite, 1250 kg/m 3 of sand and 100 kg/m 3 of MPCM. This study shows that the application of the MPCMs cannot only further reduce the thermal conductivity of PWS but also endow PWS with the function of heat regulation. A broad prospect of the application of MPCMs in underground cooling engineering can be expected.
研究领域:
土木工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）