SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Yunlei Zhou,Fei Li,Hanwen Wu,Yan Chen,Huanshun Yin,Shiyun Ai,Jun Wang
A selective and sensitive electrochemical aptasensing strategy was developed for kanamycin detection based on target-triggered single-strand DNA adsorption on MoS 2 nanosheets and enzymatic signal amplification. Firstly, a Y-shape double-stranded DNA (dsDNA) was designed containing probe DNA, assist DNA (labeled with biotin at its 3′-terminal) and aptamer DNA. Then, in the presence of kanamycin, aptamer DNA can be released from the Y-shape dsDNA structure due to its interaction with kanamycin to form stable conjugate, which will destroy the Y-shape dsDNA structure and lead to the presence of two-fragment of single-stranded DNA (ssDNA) on the remained half-hybrid DNA structure (containing probe DNA and assist DNA). Based on the strong interaction between ssDNA and MoS 2 nanosheets, the half-hybrid DNA structure can be captured on MoS 2 nanosheets modified electrode surface, which triggers the subsequent immobilization of streptavidin-alkaline phosphatase (SA-ALP) compound. Under the catalysis of ALP, p -nitrophenol phosphate (PNPP) is hydrolyzed to produce p -nitrophenol (PNP). Based on the linear relationship between the electrochemical oxidation signal of PNP and kanamycin concentration, kanamycin can be detected with the detection limit of 0.029 nM (S/N = 3). The aptamer-MoS 2 nanosheets based electrochemical biosensor shows good selectivity, reproducibility for kanamycin detection.
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Qisen Xiang,Xiufang Liu,Junguang Li,Shengnan Liu,Hua Zhang,Yanhong Bai
This work aimed to evaluate the effects of dielectric barrier discharge (DBD) plasma on inactivation of spoilage yeast Zygosaccharomyces rouxii ( Z. rouxii ), in apple juice. Results showed that DBD plasma treatment at 90 W for 140 s resulted in about 5-log reduction of Z. rouxii in apple juice. The levels of extracellular nucleic acids and proteins as well as contents of H 2 O 2 and NO 2 − in yeast extract-peptone-dextrose (YPD) medium increased significantly after DBD plasma treatment at 90 W for 40–200 s. The increases in membrane permeability and generation of reactive species would likely contribute to DBD plasma-mediated inactivation of Z. rouxii . DBD plasma caused significant changes in pH, titratable acidity, and certain color parameters of apple juice, but had no effect on the contents of total soluble solids, reducing sugar, and total phenolics. This study provides key implications for the application of DBD plasma in fruit juice processing.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2018
·Vol. 410
·期 11
·2665-2669
·
IF 3.307
Zhang Xiu-Xiu,Song Yi-Zhen,Fang Fang,Wu Zhi-Yong
On-site rapid monitoring of nitrite as an assessment indicator of the environment, food, and physiological systems has drawn extensive attention. Here, electrokinetic stacking (ES) was combined with colorimetric reaction on a paper-based device (PAD) to achieve colorless nitrite detection with smartphone. In this paper, nitrite was stacked on the paper fluidic channel as a narrow band by electrokinetic stacking. Then, Griess reagent was introduced to visualize the stacking band. Under optimal conditions, the sensitivity of nitrite was 160-fold increased within 5 min. A linear response in the range of 0.075 to 1.0 μg mL −1 ( R 2 = 0.99) and a limit of detection (LOD) of 73 ng mL −1 (0.86 μM) were obtained. The LOD was 10 times lower than the reported PAD, and close to that achieved by a desktop spectrophotometer. The applicability was demonstrated by nitrite detection from saliva and water with good selectivity, adding 100 times more concentrated co-ions. High recovery (91.0~108.7%) and reasonable intra-day and inter-day reproducibility (RSD < 9%) were obtained. This work shows that the sensitivity of colorless analyte detection-based colorimetric reaction can be effectively enhanced by integration of ES on a PAD.
Schematic of the experimental setups (left) and the corresponding images (right) of the actual portable device.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Hydro-environment Research
·
2018
·
IF 2.087
Pengjun Li,Kairong Lin,Zhanqiang Fang,Wenfeng Zhang
In this study, degradation of nitrate in drinking water by nanoscale zero-valent iron prepared from steel pickling waste liquor(S-NZVI) was investigated. Transmission Electron Microscope (TEM), Scanning Electron Microscope (SEM) and Specific Surface Area Analyzer (BET) were used to characterize the properties of the synthesized nanoparticles. Effects of different parameters, such as S-NZVI dosage, initial nitrate concentration, and pH, on the denigration were conducted. The results showed that S-NZVI had the similar characteristic of the NZVI prepared by chemical agents. The experiments indicated that the slightly acid medium with the S-NZVI dosage of 2.8 g/L had a better removal effect of nitrate, and the high removal efficiency of 96% was observed after the reaction time of 2 h, which demonstrated it was feasible and effective to remove nitrate by S-NZVI. The kinetic studies indicated that the degradation rate of nitrate by S-NZVI followed the pseudo-first order kinetics. By analyzing the end-products of nitrate reduction, 94% of nitrate was converted into ammonia ions by S-NZVI and the others translated into a little amount of nitrite and nitrogen. It is a relatively easy and cost-effective method for nitrate removal, so S-NZVI has a great potential application in nitrate removal of drinking water. The ammoniac nitrogen pollution, the main product and second pollution from the denitrification by S-NZVI can be completely degraded by cation exchange resin. So the nitrogen pollution has been completely removed from drinking water polluted by nitrate.
✓ 已验证
产品关联
DOI
高影响因子
LAB ON A CHIP
·
2018
·Vol. 18
·期 19
·2994-3002
·
IF 5.995
Y. Shi,H. L. Liu,X. Q. Zhu,J. M. Zhu,Y. F. Zuo,Y. Yang,F. H. Jiang,C. J. Sun,W. H. Zhao,X. T. Han
Nitrite detection plays a very important role in environmental monitoring and for industrial purposes. The commonly used colorimetric analysis requires the measurement of a system's calibration curve by asynchronously preparing and detecting a dozen standard samples, leading to time-consuming, slow and cumbersome procedures. Here, we present a differential colorimetry method that determines the nitrite level based on the paired chromaticity gradient, formed by coupling the colour reaction into the microfluidic network. The two gradients reshape each other and contain enough information for the quantitative analysis of the sample being tested, without the need for a calibration curve. The independence of the two gradients of the absorbance change caused by the detecting system and water quality results in a high stability and anti-interference performance, with the assistance of its self-correcting ability. This differential colorimetry method requires little time and energy consumption as only one sample is needed. Standard nitrite solutions of 0.50 mM and 0.33 mM have been determined with an error of 1.16% and 0.50%, respectively. These measurements are advantageous in terms of greater stability by up to 10 times and accuracy by 6 times, compared with the calibration curve approaches. It is foreseeable that this differential colorimetry method will find a wide range of applications in the field of chemical detection.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Catalysis
·
2025
·
IF 13.1
Jieying Wan,Ji Yang,Na Yang,Yifei Sun,Chuansheng Hu,Yang Zhao,Xiaoyan Xu,Haifeng Qi,Xiaodong Li,Hao Zhang
Electrochemical nitrate reduction reaction (NO3–RR) presents a sustainable method for ammonia synthesis. Single-atom catalysts possessing the symmetric planar four-ligand structure (M-N4) serve as advantageous catalytic active sites for NO3–RR. However, the inherent extreme symmetry of the standard M-N4 structure limits the reaction kinetics. Herein, we introduce a symmetry-breaking iron single-atom catalyst coordinated with axial chlorine on nitrogen-doped carbon (Cl-Fe-NC) for NO3–RR. Cl-Fe-NC exhibits a 99.4% ammonia Faradaic efficiency (FE) at −0.28 V vs reversible hydrogen electrode (RHE) with a 9396.7 μgNH3 h–1 cm–2 yield rate at −0.68 V vs RHE, remarkably surpassing that of Fe-NC (<80%, 4330.9 μgNH3 h–1 cm–2 at the same potential). Operando synchrotron radiation Fourier transform infrared (SR-FTIR) spectroscopy confirms that key intermediates, such as *NO, *NO-Hx, and σ(N–H), are formed. Density functional theory (DFT) calculations attribute the optimized free energy of NO3–RR intermediates to the axial chlorine design, reducing the potential determination step barrier energy by up to 0.66 eV. The presence of axial Cl atoms modulates the symmetry of the single Fe atom, enhancing the adsorption of nitrate ions and the enrichment of critical intermediates during NO3–RR while inhibiting the hydrogen evolution reaction (HER). This discovery opens avenues for boosting electrochemical ammonia synthesis through the precise modulation of atomic structures by doping heteroatoms for symmetry breaking.
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry A
·
2025
·
IF 9.5
Ru Jia,Xiaoxue Zhang,Li Gan,Muhammad Tahir,Zhen-Feng Huang,Lun Pan,Ruijie Gao,Chengxiang Shi,Xiangwen Zhang,Guidong Yang,Ji-Jun Zou
The nitrate reduction reaction (NO3RR) represents a promising route for water treatment and NH3 generation. This process involves the deoxygenation of NO3− to form nitrite (NO2−), followed by its subsequent hydrogenation. However, discrepancies in the rates of these two steps result in a decrease in faradaic efficiency (FE) and NH3 yield rate. Herein, we demonstrated a tandem catalyst of (Cu7/Ag3)7-Ru3/C achieving a high NH3 yield rate of 3.45 mmol h−1 cm−2 (2.30 mol gcat−1 h−1) and a FE of 93.48% at −0.9 V vs. the reversible hydrogen electrode. The Cu/Ag heterostructure greatly enhanced the conversion of NO3− to NO2− over a wide potential window due to the synergistic effect, while Ru, selectively adsorbing NO2−, provided active hydrogen derived from water hydrolysis to facilitate NH3 synthesis. Furthermore, (Cu7/Ag3)7-Ru3/C exhibited stable performance in a membrane electrode assembly over 60 hours, achieving an average NH3 yield rate of 6.90 mmol h−1. The ammonium chloride solid product was successfully obtained using an air stripping methodology. In situ characterization revealed that the surface microenvironment of Ru influenced the adsorption configuration of *NO and on-top adsorbed NO was more favorable for ammonia synthesis compared to bridge-adsorbed NO. The overall reaction pathway involved stepwise deoxygenation to form *N and subsequent gradual hydrogenation.
✓ 已验证
产品关联
DOI
高影响因子
CHEMICO-BIOLOGICAL INTERACTIONS
·
2024
·
IF 4.7
Changfeng Zhong,Changmei Wang,Wei Li,Wenyuan Li,Xuemei Chen,Jieqing Guo,Yifan Feng,Xia Wu
Parkinson's disease (PD) is the fastest growing neurodegenerative disease in the world at present. Neuroinflammation plays an important role in Parkinson's disease. In our study, we initially screened magnolol/honokiol derivatives synthesized by our group for their potential anti-neuroinflammatory properties. This was done using LPS-activated BV-2 microglial cell and MPP + -induced PC-12 cell models. Most of derivatives had increased anti-inflammatory activities and decreased toxicities compared to raw materials. Then, compounds were scored with inflammatory factors IL-1β, TNF-α and IL-6 by molecular docking in silico . Our studies revealed the strongest binding compound HM568 which binds with honokiol and metformin. Furthermore, HM568 showed no acute toxicity in mice through acute toxicity. And it is stable under high temperature, high humidity and strong light irradiation. Combining cell experiments and computer results, HM568 was considered for further in vivo pharmacological validations. Intraperitoneal injection administration of MPTP into C57BL/6 mice was utilized as Parkinson's animal model. Results showed that administration of HM568 for 14 days in MPTP-PD mice led to a significant alleviation in weight loss and movement disorders. Further HM568 could significantly down-regulate the expression levels of inflammatory factors IL-1β, IL-6 and TNF-α in brain tissue of the mouse model, reduce the level of caspase-3 and the ratio of Bcl-2/Bax, and up-regulate the level of transforming factor TGF-β, thus producing anti-apoptosis and anti-neuroinflammatory effects on neuronal cells. In terms of pathological features, HM568 could reduce the infiltration of neuronal cells and alleviate the development of lesions, promote the transformation of microglia from M1 negative phenotype to M2 type, and reverse the reduction of TH-positive immune cells in mouse neurons induced by MPTP. The administration of HM568 could reduce the abnormal accumulation of α-syn, and thus produce neuroprotective effect on MPTP-PD mice. Cell experiments, molecular docking and animal experiments thus depict HM568 as a promising agent to delay neuronal degeneration in PD, and its mechanism is related to anti-neuroinflammation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Catalysis
·
2025
·
IF 13.1
Hengyuan Liu,Yongqi Jia,Xintong Huang,Yingzhe Liu,Qiang Yang,Zhuo Chen,Jianhong Xu
The electrocatalytic nitrate reduction reaction (NitrRR) has attracted great attention in clean ammonia production, but it has unsatisfactory selectivity and sluggish dynamics, owing to the complex eight-electron transfer process. While dendritic AuCu alloy is anticipated to offer competitive performance, significant challenges remain in terms of insufficient structural regulation and an unelucidated reaction enhancement mechanism because of the complexity involved in its preparation. To address these issues, we have developed a two-stage microfluidic platform that facilitates the stable fabrication and controllable regulation of AuCu nano dendrites (NDs). Notably, the Cu content in the resultant AuCu NDs reaches an impressive 35.34 At%, surpassing traditional liquid-phase reduction limitations. Furthermore, the dendrite structure has been thoroughly validated, revealing a clear structure–activity relationship. By employing precise manipulation, we have determined the optimal composition of AuCu NDs, achieving a remarkable ammonia yield of 21.93 mg h–1 cm–2 and a faradic efficiency of 93.30%. Additionally, DFT calculations further elucidate the performance enhancement mechanism, showing that Au3Cu1 sites in the AuCu NDs significantly reduce the energy barrier (0.28 eV) of the rate-determining step (RDS: *NO → *HNO), while excessive Cu deposition has an adverse effect. Our work contributes innovative guidance for the design and controllable fabrication of high-performance electrocatalysts.
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2015
·Vol. 3
·期 24
·4904-4912
·
IF 4.726
Jiangsheng Xu,Fang Zeng,Hao Wu,Shuizhu Wu
Compared to the generation of singlet oxygen in photodynamic therapy, photo-generation of nitric oxide (NO) would not be limited by the concentration of molecular oxygen. However, therapeutic applications of exogenous nitric oxide are usually limited by its short half-life and its vulnerability to many biological substances, thus straightforward and precise control over NO delivery may be critical to its therapeutic effects. Herein, we demonstrate a mitochondrial-targeting and photoactive NO-releasing system as an anticancer drug. Fabricated by covalently incorporating a photo-responsive NO-donor and a mitochondrial targeting ligand onto carbon dots, this nanosystem exhibits a multi-functional nature which combines mitochondrial-targeting, photocontrollable NO-releasing and cell imaging. Upon cellular internalization, the nanosystem could target mitochondria effectively. Furthermore, the system displays little dark toxicity under physiological temperature; but upon light irradiation, it could release NO, efficiently damage mitochondria and consequently cause prominent apoptosis of cancer cells. Moreover, evaluated by using MTT assay, this nanosystem shows high cytotoxicity towards two cancer cell lines. These observations provide new insights for exploiting NO in disease therapy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2015
·
IF 5.636
Tianwei Lv,Hongyuan Yan,Jiankun Cao,Shiru Liang
Hydrophilic molecularly imprinted resorcinol–formaldehyde–melamine resin (MIRFM) is synthesized in water and shows excellent molecular recognition in aqueous matrices. The double functional monomers resorcinol and melamine, and the cross-linker formaldehyde, are all hydrophilic, and then the hydrophilic groups (such as hydroxyls, imino groups, and ether linkages) can be introduced into MIRFM, which make the material compatible with aqueous samples. The general principle is demonstrated by the synthesis of MIRFM using sulfanilamide as a dummy template for the selective recognition to sulfonamides (SAs) in milk samples. Resorcinol and melamine can interact with the template mainly by hydrogen bonding and π–π interaction, which makes MIRFM and the analytes have strong affinity. Besides, melamine can improve the rigidity of MIRFM and accelerate the polymerization process, so there is no need to add base or acid as a catalyst, which guarantees the success of molecular imprinting. MIRFM shows higher recovery and improved purification effect for SAs, in comparison to silica, HLB, C18, and SCX. Because of its excellent hydrophilicity and specificity, MIRFM is promising to be applied in biological, environmental, and clinical fields.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Cleaner Production
·
2017
·
IF 5.715
Pengjun Li,Kairong Lin,Zhanqiang Fang,Kangming Wang
In this study, a novel bimetallic iron/nickel nanoparticles supported on biochar (BC@Fe/Ni) was established to degrade nitrate pollution in water. The nanocomposite was prepared from steel pickling waste liquor and sugarcane bagasse, which are made at low cost. The particle sizes of the nanocomposite ranged from about 10 nm to 20 nm and its specific surface area (59.83 m 2 /g) was about 71% bigger than that of nanoscale zero valent iron prepared from steel pickling waste liquor (S-NZVI), which proved that biochar had an excellent dispersal effect on bimetallic iron/nickel particles. Without controlling the pH, high nitrate removal rates over 93% could be realized in nitrate concentrations below 50 mg/L. The results of batch experiments demonstrated that the kinetics curves fitted the pseudo-first-order reaction well. And it was also found that higher dosages of the nanocomposite, lower initial nitrate concentrations, and acid medium facilitated nitrate degradation. In addition, the observed pseudo-first order rate coefficient in nitrate (20 mg/L) degradation rate by 4 g/L of the bimetallic iron/nickel supported on biochar was 30% faster than that by 2 g/L the bimetallic iron/nickel nanoparticles , due to the existence of biochar. And it was 75% faster than that by 2 g/L the nanoscale zero valent iron due to the existence of both biochar and the nanoscale zero valent nickel catalyst. Furthermore, iron and nickel ion pollution occurred in the denitrification system with bimetallic iron/nickel nanoparticles but not in that with the bimetallic iron/nickel nanoparticles supported on biochar, due to the biochar’s adsorption. After denitrification by the bimetallic iron/nickel nanoparticles supported on biochar, most of nitrate turned into ammonia (17.04 mg/L) and there only was a small amount of nitrite (0.024 mg/L) with high nitrate removal rate (99.5%). And most of ammonia can be absorbed by cation exchange resin with finial ammonia concentration of 0.58 mg/L. At last, the concentrations of nitrate (0.24 mg/L), nitrite (0.021 mg/L), and total nitrogen (0.84 mg/L) were lowest in the nanocomposite system. Overall, the bimetallic iron/nickel nanoparticles supported on biochar exhibited clear advantages over bimetallic iron/nickel nanoparticles and nanoscale zero valent iron with respect to degrading nitrate efficiently, preventing iron and nickel pollution and removing ammonia pollution in combination with cation exchange resin .
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）