SENSORS AND ACTUATORS B-CHEMICAL
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2016
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IF 4.758
Linlin Zhao,Juan Li,Yuhong Liu,Yuan Wei,Jin Zhang,Jinzha Zhang,Qing Xia,Qiang Zhang,Wenbo Zhao,Xiaoqiang Chen
We reported a novel ECL immunosensor for detection of carcinoembryonic antigen (CEA) based on the reduced graphene Oxide-BaYF 5 :Yb, Er (rGO-BaYF 5 :Yb, Er) nanocomposites. The rGO-BaYF 5 :Yb, Er nanocomposites, used as electrode luminescence materials, were prepared by in situ hydrothermal method, and characterized by TEM, XRD, UV–vis spectra, and upconversion fluorescence spectra. Further, poly (diallyldimethylammonium chloride) (PDDA), Au nanoparticles (AuNPs), and Anti-CEA were successively assembled on the surface of rGO-BaYF 5 :Yb, Er nanocomposites modified-electrode for fabricating ECL immunosensor. The results of the ECL measurements of CEA indicated that this novel ECL immunosensor exhibited high sensitive response to CEA in a linear range of 0.001–80 ng mL −1 with a low detection limit of 0.87 pg mL −1 , good selectivity and satisfactory stability.
研究领域:
生物医学工程
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分析化学
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纳米技术
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Yilei Lu,Xiujun Rong,Peng Wu,Jian Shou,Long Chen,Fang Luo,Cuiying Lin,Jian Wang,Bin Qiu,Zhenyu Lin
The discrepancy of the electrostatic interaction of negatively charged signal molecules to long and short DNA strands of the modified electrode surface has been used for the first time to the develop an electrochemiluminescence (ECL) biosensor for human papillomavirus 16 (HPV 16) DNA detection. The short single-stranded capture probe (CP) was modified first on the surface of the gold electrode, which only has a small amount of negative charge. The electrostatic interaction between the negatively charged tris(2,2′-bipyridyl) ruthenium(II) chloride hexahydrate-doped SiO2 nanoparticles (Ru@SiO2 NPs) and CP is weak, hence Ru@SiO2 NPs easily diffuse to the surface of the electrode to generate a strong ECL signal. Hybrid chain reaction (HCR) amplification products (long strand dsDNA) were prepared in homogeneous solution in advance. When the target was present, the dsDNA can be connected on the electrode surface and cause the enhancement of the negative charge on the electrode surface. Owing to electrostatic interaction and steric hindrance, Ru@SiO2 NPs are difficult to diffuse to the electrode surface, resulting in a significantly reduced ECL signal. The decrease of ECL signal is linearly correlated with the logarithm of the HPV concentration under optimal conditions, with the detection range being 0.1 fM −5 pM with a limit of 1.41 aM. This innovative methodology expands the application of electrostatic interaction in ECL sensing, but can also easily develop biosensors for detecting other targets by changing the DNA sequence used in this strategy.
研究领域:
生物医学工程
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分析化学
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
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IF 4.8
Ziying Zhong,Yun Tang,Yanran Chen,Chunfang Wang,Faqiong Zhao,Baizhao Zeng
The widespread use of tetracycline unavoidably causes residue problems, hence its monitor in foods and environment is important. In this work, a simple but sensitive electrochemiluminescence (ECL) sensor is prepared for tetracycline detection. The sensor is constructed using TiO 2 nanoparticle, ZIF-5 loaded Ru(bpy) 3 2+ (Ru@ZIF-5), and molecularly imprinted poly-o-phenylenediamine. The TiO 2 NP acts as a co-reaction accelerator for tri-n-propylamine (TPrA) by producing electrons and holes at a certain potential to promote the production of TPrA . ZIF-5 has a large specific surface area and rich functional groups, thus can well immobilize a large amount of illuminant Ru(bpy) 3 2+ . The electrodeposited molecularly imprinted polymer acts as a recognition element. Due to the synergic effect of different components, the resulting ECL sensor shows a wide linear range of 10 nM–50 μM, high selectivity, low detection limit, and good stability. It can be used for the detection of tetracycline in food and environmental samples.
研究领域:
分析化学
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环境科学
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食品科学
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高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 414
·期 29
·8309-8315
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IF 4.478
Zhang Huixin,Du Lin,Wei Zhihao,Wang Xuemei,Sojic Neso,Zhou Xin,Wang Zonghua
Electrochemiluminescence (ECL) of luminol is a well-established methodology in analytical chemistry and bioimaging. Developing novel strategies to enhance the ECL signal of this model emitter is a challenging but rewarding task. In this work, we introduced the high-intensity focused ultrasound (HIFU), as a pretreatment means and a non-invasive way to trigger and boost the ECL signal with a 40-fold significant enhancement in the luminol-O 2 system without the addition of exogenous co-reactants. The superoxide anion (O 2 −• ) generated in situ by HIFU was the key initiator for boosting the ECL emission as demonstrated in this study for the first time. This promising co-reactant-free strategy could find potential applications for ultrasensitive ECL detection in the analysis of complex biological entities. Graphical abstract
研究领域:
分析化学
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生物成像
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超声技术
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生物分析
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
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IF 4.3
Haifeng Yao,Changbo Jia,Yongping Dong
Herein, a novel sandwich electrochemiluminescence (ECL) aptasensor was developed based on the resonance energy transfer (RET) with iridium complex doped silicate nanoparticles (SiO 2 @Ir) as energy donor and gold nanoparticles modified TiVC MXene (AuNPs@TiVC) as energy acceptor. Strong anodic ECL signal of SiO 2 @Ir was obtained through both co-reactant pathway and annihilation pathway. Electrochemical results showed that SiO 2 @Ir has good electron transfer rate and large specific surface area to immobilize more aptamers. AuNPs@TiVC apparently quenched the ECL signal of SiO 2 @Ir due to the ECL resonance energy transfer between them. In the presence of kanamycin (KAN), a sandwich type sensor was formed with the aptamer probes as connecters between the donor and the acceptor, resulting in the decrease of ECL intensity. Under the optimal condition, KAN could be sensitively detected in the range of 0.1 pg/mL to 10 ng/mL with a low detection limit of 24.5 fg/mL. The proposed ECL system exhibited satisfactory analytical performance, which can realize the detection of various biological molecules by adopting suitable aptamer.
研究领域:
分析化学
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生物传感
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纳米技术
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Mengxuan Zhang,Xuxiang Zheng,Lanyue Kou,Lizhu Xiao,Huan Cai,Tao Hu,Yujiao Ma,Anyi Chen,Min Zhao
In situ -engineered NU-1000@Gra gives 2.20-fold ECL enhancement via dual paths, surmounting single-component trade-off. • First Zr-MOF/graphene synergistically boosts ECL, lifting quantum yield from 11.89% to 15.69% via excited-state optimization. • TDN strategy enhances stability and anti-interference, enabling PD-L1 + tEV detection with LOD 899.35 particles/mL. • NGTA platform detects NSCLC with 100% sensitivity, 90.91% accuracy, 32.09-fold lower LOD than ELISA for tEV biopsy. Tumor-derived PD-L1 + extracellular vesicles (PD-L1 + tEVs) represent valuable liquid-biopsy biomarkers for non-small-cell lung cancer, whereas their reliable quantification is largely restricted by low abundance and complex matrix interference. Herein, we fabricated an in-situ engineered NU-1000@Graphene (Gra) nanohybrid as a kinetics-efficiency dual-boosted electrochemiluminescence (ECL) emitter to build the N U-1000@ G ra t etrahedral a ptasensor (NGTA). Benefiting from intimate interfacial coupling, Gra acted as a bifunctional accelerator in the NU-1000/S 2 O 8 2− system, accelerating interfacial electron transfer and sulfate radical production to realize a 1.58-fold improvement in ECL kinetics and a 1.39-fold enhancement in luminescence efficiency. Assisted by the tetrahedral DNA nanostructure scaffold that suppressed non-specific adsorption, the NGTA achieved an ultralow detection limit of 899.35 particles/mL for PD-L1 + tEVs. Preliminary clinical results demonstrated its diagnostic potential. This work underscores the significance of metal-organic framework (MOF)-Gra interfacial engineering for synergistic ECL amplification, providing a rational material design paradigm for high-performance liquid-biopsy biosensors.
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高影响因子
Sensors and Actuators Reports
·
2025
·
IF 7.6
Yanli Sun,Hongying Li,Tao Wang,Kai Zhang,Xueliang Wang
MicroRNA-17 (miR-17) is a critical biomarker linked to various cancers and diseases, underscoring the need for sensitive and selective detection methods. Herein, an ultra-sensitive electrochemiluminescent (ECL) sensor for miR-17 detection utilizing MOF-derived porous CuFe 2 O 4 nanospheres and CRISPR/Cas13a trans-cleavage-triggered hybridization chain reaction (HCR) amplification was presented. The porous CuFe 2 O 4 nanospheres possessed a high surface area and excellent catalytic activity, significantly enhancing ECL emission. To the best of our knowledge, this is the first report demonstrating that MOF-derived CuFe 2 O 4 can effectively enhance the electrochemiluminescence of Ru(bpy) 3 2 ⁺ for miR-17 detection. The CRISPR/Cas13a system provided miR-17 recognition and trans-cleavage activity, triggering HCR for signal amplification. Under optimized conditions, the biosensor exhibited a wide linear detection range from 20 aM to 5000 aM, with a detection limit of 9.23 aM. The system discriminated miR-17 from closely related miRNAs with negligible cross-reactivity, which demonstrated its outstanding selectivity. Practical applicability was confirmed by successful quantification of miR-17 in diluted human serum which achieved recovery rates between 95.2% and 104.8%. The results validated the biosensor’s potential for early disease diagnosis and clinical monitoring of miR-17-associated disorders.
研究领域:
生物传感器
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分析化学
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纳米材料
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分子诊断
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癌症研究
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产品关联
DOI
高影响因子
TALANTA
·
2020
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IF 5.339
Libo Li,Bainian Chen,Lijun Luo,Xiaohong Liu,Xiaoya Bi,Tianyan You
In the present study, a novel self-enhanced electrochemiluminescence (ECL) aptasensor which combined self-enhanced ECL composite as signal response element and aptamer as the specific recognition element was firstly proposed for the sensitive and selective detection of Hg 2+ . Innovatively, the luminophore Ru(bpy) 3 2+ -doped silica nanoparticles functionalized with 3-aminopropyltriethoxysilane (NH 2 – [email protected] 2 ) and the co-reactant nitrogen doped graphene quantum dots (NGQDs) were bound together to form the self-enhanced ECL composite, NH 2 – [email protected] 2 -NGQDs, by electrostatic adsorption. Satisfactorily, high and stable luminous efficiency of NH 2 – [email protected] 2 -NGQDs was obtained benefited from the short electron transfer distance and low energy loss of self-enhanced-typal ECL composite. For the fabrication of the self-enhanced ECL aptasensor for Hg 2+ detection, the Hg 2+ aptamer, of which one end was connected to NH 2 – [email protected] 2 -NGQDs, and the other end was fixed on glass carbon electrode (GCE) surface through Au–S bond, was served as a bridge. Upon the addition of Hg 2+ , the aptamer was bent due to the formation of thymine-Hg 2+ -thymine (T-Hg 2+ -T) specific structure, which caused the self-enhanced ECL composite was close to the GCE surface. On this basis, a linearly enhanced ECL signal was acquired with the concentration of Hg 2+ in the range of 5.0 × 10 −11 M − 1.0 × 10 −6 M with excellent selectivity , repeatability and stability in 3 min for each assay. In addition, the proposed aptasensor showed satisfying accuracy and practicability for Hg 2+ analysis in tap and canal water verified by the inductively coupled plasma-mass spectrometry (ICP-MS) method.
研究领域:
分析化学
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环境科学
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纳米技术
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电化学
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DOI
PubMed
高影响因子
ChemistrySelect
·
2024
·Vol. 9
·期 23
·e202401018
·
IF 1.9
Hai Shuai,Hanfeng Cui,Jie Zhang,Huiting Hu,Qian Long,Xiaocui Yin,Fusheng Liao,Hao Fan,Zhiqiang Liu,Bo Wu
Graphical In this study, we present the novel combination of AE-TPEA with RuMOF to construct an electrochemiluminescence (ECL) sensor using a TPEA-RuMOF composite material specifically for copper ions (Cu 2+ ) detection. Using K 2 S 2 O 8 as a co-reactant, we explored the ECL behavior of the TPEA-RuMOF modified electrode, enabling the quantitative detection of Cu 2+ . Remarkably, this sensor exhibited a wide linear range from 1×10 −5 to 1×10 −9 M (R 2 =0.998) and boasted a detection limit as low as 0.41 nM, which is considerably below the EPA (Environmental Protection Agency) permissible copper ion concentration in drinking water of <1.3 mg/L. our synthesized probe offers enhanced stability and selectivity in copper ion detection. This indicates its considerable potential for high-performance copper ion detectors. We further validated the analytical applicability of this sensor using river water samples. In this study, we present the novel combination of AE-TPEA with RuMOF to construct an electrochemiluminescence (ECL) sensor using a TPEA-RuMOF composite material specifically for copper ions (Cu 2+ ) detection. Using K 2 S 2 O 8 as a co-reactant, we explored the ECL behavior of the TPEA-RuMOF modified electrode, enabling the quantitative detection of Cu 2+ . Remarkably, this sensor exhibited a wide linear range from 1×10 −5 to 1×10 −9 M (R 2 =0.998) and boasted a detection limit as low as 0.41 nM, which is considerably below the EPA (Environmental Protection Agency) permissible copper ion concentration in drinking water of <1.3 mg/L. our synthesized probe offers enhanced stability and selectivity in copper ion detection. This indicates its considerable potential for high-performance copper ion detectors. We further validated the analytical applicability of this sensor using river water samples.
研究领域:
分析化学
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环境科学
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材料科学
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传感器
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Qiao Liu,Ying Yang,Xing-Pei Liu,Yu-Ping Wei,Chang-Jie Mao,Jing-Shuai Chen,He-Lin Niu,Ji-Ming Song,Sheng-Yi Zhang,Bao-Kang Jin,Meng Jiang
A novel highly electrochemiluminescence (ECL) metal-organic frameworks (MOFs) have been successfully synthesized by combining CdSe quantum dots (QDs) and MIL-101 in one step by facile hydrothermal method. The as-prepared MIL-101-CdSe nanocomposites combine a high ECL activity and sensing selectivity derived from CdSe and the highly selective adsorption and efficient accumulation performance of MOFs. The selective accumulation effect of MOFs can greatly amplify the sensing signal and specificity of the ECL probe. The resulting nanocomposites were applied to develop an ultrasensitive ECL immunosensor for detection of carcinoembryonic antigen (CEA). This ECL immunosensor has a sensitive response to CEA in a linear range of 10 −12 mg mL −1 –10 −4 mg mL −1 with a detection limit of 0.33 fg mL −1 . The developed ECL immunosensor shows high sensitivity, good stability, specificity, and excellent reproducibility, and can be used for detection of real samples, which providing potential application in clinical detection.
研究领域:
材料科学
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分析化学
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生物医学工程
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Hongye Wang,Feng Wang,Tao Wu,Yang Liu
Rational design of electrochemiluminescence (ECL) reagents is essential for the development of ECL biosensors with superior performances. In this work, the assembly of tris(1,10-phenanthroline)ruthenium(II) [Ru(phen)32+] and tetrahedral chalcogenide nanoclusters of [Cd32S14(SC6H5)38]2– in the formation of complex nanoclusters (CdS–Ru) was developed, in which Ru(phen)32+ was uniformly encapsulated and dispersed at a molecular level in the chalcogenide nanocluster via multiple noncovalent interactions. It was observed that the promoted ECL emission was realized by the charge transfer between the tetrahedral CdS nanocluster and Ru(phen)32+ by the formation of the assembly complex, which was elucidated by cyclic voltammetry curves, ECL–potential curves, and in situ dynamic ECL spectra. Taking advantages of the facile charge transfer in the open framework CdS–Ru, a high ECL efficiency has been achieved with remarkable stability. Moreover, a solid-state ECL sensor based on the CdS–Ru modified electrode was fabricated for label-free detection of alkaline phosphatase (ALP) activity with a detection limit as low as 0.35 U/L and superior reproducibility. This solid-state ECL sensor also displayed favorable selectivity among various interferences and was applied for ALP activity analysis in human serum samples. These results implicated the potential applications of CdS–Ru for sensitive ECL analysis in complicated reaction systems and enlightened the rational design for self-enhanced and highly efficient ECL materials.
研究领域:
分析化学
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生物传感
·
纳米材料
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2024
·
IF 3.6
Xinyue Qian,Cheng Tan,Jing Zhang,Kang Wu,Anping Deng,Jianguo Li
The “antenna effect” is one of the most important energy transfer modes in lanthanide light-emitting polymers. In this study, novel luminescent nanostructured coordination polymers (Eu–PCP) were synthesized in one step using Eu3+ as the central metal ion and 5,10,15,20-tetrakis (4-carboxyphenyl) porphyrin (TCPP) as the organic ligand. The unique “antenna effect” observed between Eu3+ and TCPP leads to a substantial improvement in the electrochemiluminescence (ECL) emission efficiency. Eu–PCP exhibits good cathodic ECL characteristics. Additionally, Au@SnS2 nanosheets exhibit favorable electrical conductivity, biocompatibility, and a significant specific surface area. This makes them a suitable choice as substrate materials for the modification of electrode surfaces and capturing antigens. Being well known, the development of sensitive and rapid methods to detect chloramphenicol is essential for food safety. Based on this, we report a novel competitive electrochemiluminescence immunoassay to achieve ultra-sensitive and highly specific detection of chloramphenicol. The linear range was 0.0002–500 ng mL−1 and the detection limit was 0.09 pg mL−1. Apart from that, the experimental results proved that it provided a new analytical tool for the detection of antibiotic residues in food safety.
研究领域:
分析化学
·
食品科学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
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