Nanoscale
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2018
·Vol. 10
·期 14
·6611-6619
·
IF 7.233
Jinyong Hu,Meiyan Wu,Li Jiang,Zhensheng Zhong,Zhangkai Zhou,Thitima Rujiralai,Jie Ma
The association of a plasmonic nano-antenna with single-molecule FRET technique presents new prospects to investigate the dynamics of biological molecules. However, the presence of a plasmonic nano-antenna significantly modifies the FRET rate and efficiency; this makes its applicability to the prevalent single-molecule FRET experiments unclear. Herein, using gold nanoparticle antennas of different sizes and DNA hairpins labelled with FRET pairs (Cy3 and Cy5) as the model system, we performed experiments to study the folding dynamics of single DNA hairpins at various salt concentrations. Our results indicate that gold nanoparticle antennas can enhance single-molecule fluorescence of Cy3 and Cy5 up to 3–5 folds, substantially reduce the FRET efficiency, and alter the obtained FRET efficiency histograms. However, the folding dynamics of DNA hairpins remains unaffected, and the correct kinetic and dynamic information can still be extracted from the seriously modified FRET efficiencies. Therefore, our experiments demonstrate the feasibility and compatibility for applying plasmonic nano-antennas to the mostly used single-molecule FRET assays, which provide a broad range of possibilities for the future applications of these nano-antennas in studying various essential biological processes.
研究领域:
生物物理
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分子生物学
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纳米技术
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PubMed
高影响因子
ACS Applied Nano Materials
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2023
·
IF 5.9
Rui Rui Yang,Pin Wang,Fangrui Cheng,bang lan,Yuxiang Yu,Jing Wang,Shi Ye
It is essential for luminescent materials to be compatible with complex bioenvironments when used for probing biological events. Förster resonance energy transfer (FRET) is a common process in gaining enhanced luminescence signals, in which the interaction between the donor and acceptor plays a decisive role in the optical properties of heterostructured bioprobes. Yet systematic investigations on the gradient amphiphilic and electrostatic interaction effect on luminescence in a complex environment are scarce. Heteronanostructures of Tb(C12H8N2)2(NO3)3 nanocrystals and Eu3+-doped oxide nanosheets in various solutions with varied double electric layers and amphiphilic capacities are chosen for the investigation. It is found that a complex redox process takes place and an interfacial Tb–O bond is formed during preparation of the heteronanostructures. According to the decay dynamics of Tb3+ and Eu3+ emission of the heteronanostructures in various amphiphilic alcohol solvents, the interfacial Tb3+ → Eu3+ energy transfer efficiency apparently displays an extraordinary dependence on alkyl chain length after excluding the refractive index effect, while the zeta potential data suggest that the interfacial electrostatic force on the heteronanostructures should be dominant via the electric double layer in alcohol solvents with short alkyl chain. As the length of alkyl chain increases, the amphiphilic force on the heteronanostructures with amphiphilic Tb(phen)2(NO3)3 and hydrophilic Eu3+-doped nanosheets tends to be overwhelming. These competitive forces strengthen the interaction between Tb3+ and Eu3+ and result in enhancement of the interfacial Tb3+ → Eu3+ energy transfer efficiency. The research helps design potential new bioprobes concerning the interfacial interaction in amphiphilic and salty biosystems.
研究领域:
材料科学
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生物医学工程
·
光学
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2014
·
IF 5.825
Jiaoyun Xia,Meihua Lin,Xiaolei Zuo,Shao Su,Lianhui Wang,Wei Huang,Chunhai Fan,Qing Huang
Contamination of heavy metal ions in an aquatic environment poses a serious threat to human health. More seriously, heavy metal ions are usually present in the environment in a mixture, and the synergetic toxicity of multiple heavy metal ions is revealed (Aragay et al. Chem. Rev. 2011, 111, 3433; Chu et al. Aquat. Toxicol. 2002, 61, 53). Unfortunately, most of the existing methods based on DNA sequences are focusing on the detection of one type of metal ions. Simple and multiplexed detection of multiple metal ions has been poorly investigated and remains challenging. Here, we re-engineered the DNA sequences for Pb2+, Hg2+, and Ag+, through which the binding of multiple metal ions initiated the self-assembly of these DNA sequences. On the basis of our rationally designed multicolor fluorescent labeling of the DNA sequences, cascade fluorescence resonance energy transfer (FRET) occurred. As a result, a fingerprint fluorescent spectrum was produced to indicate the presence of a single type of metal ions or multiple metal ions. The major advantages of our cascade FRET fingerprint technology include the following: (1) the “mix and read” detection mode in homogeneous solution is simple without the need of complicated instruments; (2) only single excitation is required to provide the cascade FRET fingerprint spectrum; (3) multiplexed detection capability can be realized intuitively and sensitively.
研究领域:
环境科学
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分析化学
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纳米技术
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生物化学
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Gan Wu,Zhaoyang Ding,Xilin Dou,Ze Chen,Jing Xie
Histamine has been known as a momentous cause of biogenic amine poisoning. Therefore, the content of histamine in foods is strictly required to be controlled within a certain range. Here, an aptamer fluorescent sensor was developed for detection of histamine. Poly [(9, 9-di-n-octylfluorenyl-2, 7-diyl)-alt-(benzo [2,1,3] thiadia-zol-4, 8-diyl)] (PF8BT) and the styrene maleic anhydride copolymer (PSMA) were used for the preparation of PF8BT-Polymer dots (PF8BT-Pdots). PF8BT-Pdots and the cyanine3-phosphoramidite (Cy3) were linked through aptamer to achieve the ratiometric detection for histamine. PF8BT-Pdots were partly quenched by Cy3 due to the fluorescence resonance energy transfer (FRET), when the histamine molecule was recognized by aptamer on the surface of PF8BT-Pdots. A linear range (3–21 μmol/L) was obtained for histamine detection with a low limit of detection (LOD = 0.38 μmol/L). PF8BT aptamer Pdots (PF8BT-A) were used to detect histamine in simply treated aquaculture water and tuna. The cell imaging of HeLa cells presented a good biosecurity and outstanding fluorescent imaging capability of PF8BT-A. The aptamer fluorescent sensors provided a new platform for rapid and accurate detection of histamine in aquatic products and had great potential for the application in food safety and quality control.
研究领域:
食品安全
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生物化学
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分析化学
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DOI
PubMed
高影响因子
ANALYST
·
2023
·
IF 4.2
Jia Chen,Shengrong Yu,Zhiling Qian,Kangdi He,Bingqian Li,Yuting Cao,Keqi Tang,Shengjia Yu,Yong-Xiang Wu
Rapid, simultaneous, and sensitive detection of biomolecules has important application prospects in disease diagnosis and biomedical research. However, because the content of intracellular endogenous target biomolecules is usually very low, traditional detection methods can't be used for effective detection and imaging, and to enhance the detection sensitivity, signal amplification strategies are frequently required. The hybridization chain reaction (HCR) has been used to detect many disease biomarkers because of its simple operation, good reproducibility, and no enzyme involvement. Although HCR signal amplification methods have been employed to detect and image intracellular biomolecules, there are still false positive signals. Therefore, a target-triggered enzyme-free amplification system (GHCR system) was developed, as a fluorescent AND-gated sensing platform for intracellular target probing. The false positive signals can be well avoided and the accuracy of detection and imaging can be improved by using the design of the AND gate. Two cancer markers, GSH and miR-1246, were used as two orthogonal inputs for the AND gated probe. The AND-gated probe only works when GSH and miR-1246 are the inputs at the same time, and FRET signals can be the output. In addition to the use of AND-gated imaging, FRET-based high-precision ratiometric fluorescence imaging was employed. FRET-based ratiometric fluorescent probes have a higher ability to resist interference from the intracellular environment, they can avoid false positive signals well, and they are expected to have good specificity. Due to the advantages of HCR, AND-gated, and FRET fluorescent probes, the GHCR system exhibited highly efficient AND-gated FRET bioimaging for intracellular endogenous miRNAs with a lower detection limit of 18 pM, which benefits the applications of ratiometric intracellular biosensing and bioimaging and offers a novel concept for advancing the diagnosis and therapeutic strategies in the field of cancer.
研究领域:
生物化学
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分子生物学
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医学诊断
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2020
·
IF 3.594
Yong Li,Xuan Li,Hongliang Tan,Zhen-Zheng Huang
In this work, a turn-on fluorescent assay based on carbon dots/manganese dioxide nanosheet (CDs/MnO 2 ) assembly was developed for the detection of glucose. The fluorescence of CDs was first quenched by the addition of MnO 2 nanosheet to form CDs/MnO 2 assembly, which leads to a fluorescence resonance energy transfer (FRET) process from CDs to MnO 2 nanosheet. When H 2 O 2 was introduced into the quenched CDs solution, the MnO 2 nanosheet was destroyed due to the redox reaction between H 2 O 2 and MnO 2 nanosheet, and the fluorescence of CDs was recovered accordingly. On this basis, by coupling with the oxidation reaction of glucose catalyzed by glucose oxidase, which produces gluconic acid and H 2 O 2 , a fluorescent assay for the quantitative analysis of glucose can be proposed. Under optimal conditions, the fluorescent intensity of CDs increased linearly with increasing concentrations of glucose from 2 to 200 μM, and the detection limit towards glucose was calculated to be 0.83 μM. In addition, the proposed fluorescent assay was successfully applied to the determination of glucose in serum samples with satisfactory results. This proposed fluorescent assay is simple, cost-effective and environmentally friendly, and thereby holding great potential for diabetes management and biosensing applications.
研究领域:
生物化学
·
医学诊断
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DOI
高影响因子
Journal of Pharmaceutical Analysis
·
2025
·
IF 8.9
Yuexiang CHANG,Hao TIAN,Jia-Huan QU,Jiaming YUAN,Rongkai GU,TingTing ZHANG,Jincai WANG,Zhengjin JIANG
The N -terminal domain of influenza viral polymerase (PA N ), a highly conserved region with critical catalytic function related to viral RNA replication and transcription, is considered as a very promising anti-influenza drug target. There is an urgent need for highly efficient and rapid screening methods to identify potential PA N inhibitors (PA N Is) from complex matrices. In this work, a novel high-throughput screening (HTS) platform was established by coupling high performance liquid chromatography and high-resolution mass spectrometry (HPLC-HRMS) with a fluorescence resonance energy transfer (FRET)-based endonuclease activity assay through an at-line nanofractionation system (ANF). The proposed screening platform could rapidly identify potential PA N Is from plant extracts with good sensitivity (baloxavir at the half maximal inhibitory concentration (IC 50 ) could be detected) and reliability ( Z ’ factor of 0.77). This platform was then successfully applied to the screening of potential inhibitors against PA N /PA N I38T from an aqueous extract of Artemisiae Argyi and 17 potential PA N Is were identified. Among them, three compounds (cynarine, isochlorogenic acid B and isochlorogenic acid C) showed comparable inhibitory activity against PA N and even better activity against PA N I38T compared to baloxavir. This study not only established a novel high-throughput ANF-based PA N Is screening platform, but also proved the feasibility to discover PA N Is from complex TCMs, which has a great potential in future anti-influenza drug discovery.
研究领域:
病毒学
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药物筛选
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植物药理学
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生物分析技术
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2018
·
IF 3.997
Xue Gao,Bing Zhang,Qiang Zhang,Yiwei Tang,Xiuying Liu,Jianrong Li
As a rising star in the family of fluorescent material, graphene quantum dots (GQDs) have attracted great attention because of their excellent properties such as high photostability against photobleaching and blinking, biocompatibility, and low toxicity. Herein, strongly fluorescent GQDs have been prepared by one-step solvothermal method. Then three kinds of GQDs-porphyrin composites were assembled by means of noncovalent interaction. It was found that the functional groups on the peripheries of the porphyrin were important on the combination mode of porphyrin and GQDs. The fluorescence of GQDs was quenched in the presence of the porphyrin . The suggested quenching mechanism is through Förster resonance energy transfer (FRET). Tetrasodium-meso-tetra(4-sulfonatophenyl) porphine dodecahydrate (NaTSPP) was easy to react with GQDs and flat on the GQDs due to the strong hydrogen bond and π-π interaction. The photoelectronic activity of the GQDs-NaTSPP composite was higher than those of the other two GQDs-porphyrin composites. These results provided some hints for how to adjust the properties of the GQDs-porphyrin composites by modifying the combination mode between porphyrins and GQDs. These novel GQDs-porphyrin composites hold promise for various optical and luminescence-based applications.
研究领域:
材料科学
·
光学
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DOI
高影响因子
ORGANIC & BIOMOLECULAR CHEMISTRY
·
2017
·Vol. 15
·期 28
·6006-6013
·
IF 3.564
Liangbin Lin,Xiaoru Lin,Hongyu Guo,Fafu Yang
This study focuses on the construction of novel diphenylacrylonitrile-connected BODIPY dyes with high fluorescence in both solution and an aggregated state by combining DRET and FRET processes in a single donor–acceptor system. The first BODIPY derivatives with one, two, or three AIE-active diphenylacrylonitrile groups were designed and synthesized in moderate yields. Strong fluorescence emissions were observed in the THF solution under excitation at the absorption wavelength of non-emissive diphenylacrylonitrile chromophores, implying the existence of the DRET process between the dark diphenylacrylonitrile donor and the emissive BODIPY acceptor. In the THF/H2O solution, the fluorescence intensity of the novel BODIPY derivatives gradually increased under excitation at the absorption wavelength of diphenylacrylonitrile chromophores, suggesting a FRET process between diphenylacrylonitrile and BODIPY moieties. A greater number of diphenylacrylonitrile units led to higher energy-transfer efficiencies. The pseudo-Stokes shift for both DRET and FRET processes was as large as 190 nm.
研究领域:
化学
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材料科学
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光学
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DOI
PubMed
高影响因子
Food & Function
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2017
·Vol. 8
·期 12
·4744-4751
·
IF 3.247
Lin Cao,Xunyu Song,Yukun Song,Jingran Bi,Shuang Cong,Chenxu Yu,Mingqian Tan
Naturally occurring nanoparticles may be present in food. In this paper we report the discovery of fluorescent nanoparticles (FNs) in Chinese mature vinegar. The physicochemical properties of these FNs were investigated. The FNs were shown to emit bright blue fluorescence under the irradiation of ultraviolet light, with a fluorescence quantum yield of 5.71%. They are highly soluble in aqueous solution, and exhibit excitation-dependent emission behavior. TEM characterization showed that the average size of FNs is about 1.40 ± 0.40 nm, and the morphology of FNs is mainly spherical. The physicochemical properties of FNs are similar to those of artificially synthesized carbon dots. Furthermore, the interaction between FNs and dopamine was studied, and the fluorescence of FNs varied gradually from a blue, to a cyan and finally to a green color with increasing dopamine concentration. The fluorescence decay dynamics revealed that Förster resonance energy transfer (FRET) occurred between the FNs and polymerized dopamine. This result suggests that the FNs from Chinese mature vinegar can interact with dopamine, which may add a new perspective to the potential health implications of foodborne nanoparticles.
研究领域:
食品科学
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生物化学
·
纳米技术
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DOI
PubMed
高影响因子
Analytical Methods
·
2023
·
IF 3.1
Danni Qin,Yaqi Han,Hao Jiang,Liang Hu
We synthesized a fluorescence ratiometric probe by combining coumarin and rhodamine B with ethylenediamine to sense Fe3+ and measure ionizing radiation doses. The presence of Fe3+ caused rhodamine to transition from a closed helical structure to an open-ring structure. Additionally, fluorescence resonance energy transfer (FRET) occurred between coumarin and rhodamine B. As a result, the fluorescence intensity at 405 nm (I405) due to coumarin was decreased, whereas that at 585 nm (I585) derived from open-ring structure rhodamine B was increased. The ratio of I585 and I405 (I585/I405) linearly increased as the Fe3+ concentration increased. The probe sensed Fe3+ in a 0–110 μM range, with a lower limit of detection (LOD) of 0.226 μM. Inspired by Fricke dosimeters, we extended the probe to measure X-ray doses using the fluorescence methodology. The probe measured X-ray doses in a 0–30 Gy range with a lower LOD of 0.5 Gy. Additionally, the dosing capability was independent of the dosing rates. Our probe showed potential for detecting Fe3+ and measuring ionizing radiation doses.
研究领域:
分析化学
·
放射生物学
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DOI
PubMed
高影响因子
Biomolecules
·
2025
·Vol. 15
·期 6
·841
·
IF 4.8
Yanyan Xue,Yi Sun,Yichun Xia,Xiuming Liu,Hua Dai
Riboswitches regulate gene expression through intricate dynamic conformational transitions, with divalent cation Mg2+and their ligands playing pivotal roles in this process. The dynamic structural mechanism by which the S-adenosyl-L-methionine (SAM) responsive SAM-VI riboswitch (riboSAM) regulates the downstream SAM synthase gene translation remains unclear. In this study, we employed position-selective labeling of RNA (PLOR) to incorporate Cy3-Cy5 into designated positions of riboSAM, applying single-molecule Förster resonance energy transfer (smFRET) method to track its conformational switches in response to Mg2+and SAM. smFRET analysis revealed that in the absence of Mg2+and ligand, riboSAM predominantly adopted a translation-activatingapoconformation. Physiological concentrations of Mg2+induced riboSAM to fold into dynamictransit-pandholo-pstates, creating a transient and structurally pliable binding pocket for ligand binding. SAM binding locks the dynamictransit-pandholo-pstates into their final stabletransitandholoconformations through conformational selection, turning off downstream cis-gene expression and completing feedback regulation of cellular SAM concentration. The observed synergistic regulatory effect of Mg2+ions and ligand on riboSAM’s conformational dynamics at single-molecule resolution provides new mechanistic insights into gene regulation by diverse riboswitch classes.
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高影响因子
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