ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Huan Du,Fengrong Lv,Renye Yue,Yuzhen Yu,Xinyu Xu,Zhe Li,Baode Chen,Cheng Zhang,Yu Peng,Wei Cheng,Sulai Liu,Xiaobing Zhang,Guosheng Song
Adenosine triphosphate (ATP) is a central mediator of cancer cell metabolism and signaling, driving processes such as proliferation, invasion, metastasis, and therapeutic resistance. Current ATP imaging modalities-including fluorescence, chemiluminescence/bioluminescence, photoacoustic imaging, and magnetic resonance imaging (MRI)-face inherent trade-offs among sensitivity, spatial resolution, and tissue penetration capability. Here, a dual-mode probe is reported that synergistically couples the anatomical resolution of MRI with the high-sensitive, long-lasting signals of afterglow imaging for visualization of ATP in the tumor microenvironment. The probe is constructed via DNA hybridization between an afterglow unit and a T 1 -weighted MRI contrast agent, with an ATP-specific aptamer mediating probe disassembly upon ATP binding. This design generates simultaneous turn-on afterglow and MRI signals, enabling the imaging of intracellular and extracellular ATP fluctuations. Metabolic modulators-coenzyme Q10 (an ATP activator) and 2-deoxy-D-glucose (2-DG, a glycolysis inhibitor)-are applied to demonstrate dynamic, bidirectional tracking of ATP levels in vivo. Furthermore, by monitoring ATP depletion during synergistic metabolic therapy (cisplatin + 2-DG), a correlation between imaging readouts and therapeutic efficacy is established. The work provides anintegrated MRI/afterglow imaging platform for high-contrast, real-time ATP sensing in cancer, offering a powerful tool for elucidating tumor metabolism and evaluating metabolic interventions.
研究领域:
分子成像
·
癌症代谢
·
生物医学工程
·
纳米医学
·
诊断影像学
✓ 已验证
产品关联
DOI
高影响因子
Nano Today
·
2021
·
IF 20.722
Weiqiang Lin,Hongxing Liu,Lingwu Chen,Junxing Chen,Dong Zhang,Qingqing Cheng,Fang Yang,Qinsong Zeng,Tianfeng Chen
Bladder tumor intravesical instillation is usually compromised by hypoxia and low target efficiency. Herein, a nanoscale oxygen generator ( [email protected] ) encapsulating superparamagnetic iron oxide nanoparticles (SPIONs) and organoselenium (SeD) with PLZ4 peptide as the BCa-targeting moiety was constructed for intravesical instillation to overcome the resistance caused by hypoxia and achieve the real-time MR imaging-guided theranosis of patient-derived bladder tumors. The real-time monitoring of intact bladders excised from 3 BCa patients showed that [email protected] could not only precisely target the tumor region inside the bladder but also specifically enhance MRI contrast efficacy. As expected, [email protected] could efficiently accumulate in BCa cells and could “infect” different batches of cells, thus enhancing its tumor permeability. Moreover, [email protected] consumed excess H 2 O 2 inside the tumor through the Fenton reaction, which relieved hypoxia resistance and effectively inhibited the proliferation of BCa cells. Taken together, these results suggest that the nanoscale oxygen generator [email protected] hold potential for application in MRI-guided preclinical BCa theranosis by ameliorating hypoxic conditions and elevating chemotherapeutic efficacy.
研究领域:
肿瘤学
·
纳米技术
·
医学影像
·
癌症治疗
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Yaqin He,Caizhi Lv,Xiandeng Hou,Lan Wu
Taking advantage of the superior biocompatibility, good stability in a wide pH and temperature range, as well as its strong affinity with DNA of hydroxyapatite (HAp), tetrahedral DNA nanostructures (TDNs) conjugated with AS1411 aptamer ( anti -nucleolin overexpressed on tumor cell membranes) were employed as affinity ligands to construct a novel mono-dispersed HAp based probe with Gd 3+ doping (Apt-TDNs-GdHAp) for MR imaging. The adsorption of TDNs on the nano-HAp surface facilely accomplished the construction of the Apt-TDNs-GdHAp probes. Meanwhile, the use of hydrophilic TDNs not only favored the phase-transfer from the oil phase to the aqueous phase, but also enhanced the mono-dispersion of this probe due to the well-ordered distribution of TDNs on the surface of nano-HAp. Moreover, Apt-TDNs-GdHAp probe with a better mono-dispersion and crystalinity achieved twice higher longitudinal relaxivity (r 1 value) than that of GdHAp synthesized by microwave-assisted method (Microwave-GdHAp), exhibiting much more excellent T 1 -weighted imaging performance. With the introduction of TDNs, the stability and the tumor-targeting accessibility were also greatly improved, showing its great potential for further bio-applications.
研究领域:
纳米技术
·
生物医学工程
·
分子生物学
·
材料科学
·
医学成像
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomedical Materials
·
2024
·
IF 3.9
Chen Huang,Wentao Huang,Yixuan Meng,Chengqian Zhou,Xiaozhuan Wang,Chunyu Zhang,Yuzhen Tian,Wei Wei,Yongsheng Li,Quan Zhou,Yukuan Tang,Wenli Chen
Atherosclerosis is a chronic inflammatory disease characterized by the accumulation of cholesterol within the arterial wall. Its progression can be monitored via magnetic resonance imaging (MRI). Ultrasmall Superparamagnetic Particles of Iron Oxide (USPIO) (<5 nm) have been employed as T1 contrast agents for MRI applications. In this study, we synthesized USPIO with an average surface carboxylation of approximately 5.28 nm and a Zeta potential of -47.8 mV. These particles were phagocytosed by mouse aortic endothelial cells (USPIO-MAECs) and endothelial progenitor cells (USPIO-EPCs), suggesting that they can be utilized as potential contrast agent and delivery vehicle for the early detection of atherosclerosis. However, the mechanism by which this contrast agent is delivered to the plaque remains undetermined. Our results demonstrated that with increasing USPIO concentration during 10-100 μg/mL, consistent change appeared in signal enhancement on T1-weighted MRI. Similarly, T1-weighted MRI of MAECs and EPCs treated with these concentra-tions exhibited a regular change in signal enhancement. Prussian blue staining of USPIO revealed substantial absorption into MAECs and EPCs after treatment with 50 μg/mL USPIO for 24 h. The iron content in USPIO-EPCs was much higher (5 pg Fe/cell) than in USPIO-MAECs (0.8 pg Fe/cell). Our results confirmed that macrophages are more likely to bind to MAECs treated with AuNPs-PEI-siRNANC (control) for 72 h than to MAECs treated with AuNPs-PEI-siRNACD40 (reduced CD40 expression), thus confirming CD40 targeting at the cellular level. When USPIO-MAECs and MAECs (control) were delivered to mice (high-fat-fed) via tail vein injection respectively, we observed a higher iron accumulation in plaques on blood vessels in high-fat-fed mice treated with USPIO-MAECs. We also demonstrated that USPIO-EPCs, when delivered to high-fat-fed mice via tail vein injection, could indeed label plaques by generating higher T1-weighted MRI signals 72 h post injection compared to controls (PBS, USPIO and EPCs alone).
In conclusion, we synthesized a USPIO suitable for T1-weighted MRI. Our results have confirmed separately at the cellular and tissue and in vivo level, that USPIO-MAECs or USPIO-EPCs are more accessible to atherosclerotic plaques in a mouse model. Furthermore, the high expression of CD40 on the cell surface is a key factor for targeting and USPIO-EPCs may have potential therapeutic effects.
研究领域:
医学影像
·
心血管疾病
·
分子生物学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2020
·Vol. 12
·期 7
·4473-4490
·
IF 6.895
Jinmao Zou,Shangxiang Chen,Yaqing Li,Linjuan Zeng,Guoda Lian,Jiajia Li,Shaojie Chen,Kaihong Huang,Yinting Chen
Precise diagnosis and effective treatment are crucial to the prognosis of pancreatic ductal adenocarcinoma (PDAC). Magnetic iron oxide nanoparticles (IONPs) are superior magnetic resonance imaging (MRI) contrast agents, while antibodies are significant immunotherapy reagents. Herein, we firstly generated a novel nanocomposite combining triple single chain antibodies (scAbs) and IONPs for the detection and treatment of PDAC. Methods: Triple scAbs (scAbMUC4, scAbCEACAM6, scFvCD44v6, MCC triple scAbs) were conjugated to the surface of polyethylene glycol modified IONPs (IONPs-PEG), forming the IONPs-PEG-MCC triple scAbs nanocomposite. Characterization of the nanocomposite was performed, and its cytotoxicity, specificity, and apoptosis induction were evaluated. In vivo MRI study and anti-pancreatic cancer effect assessment were performed in tumor-bearing nude mice. Results: The size of the IONPs-PEG-MCC triple scAbs nanocomposite was about 23.6 nm. The nanocomposite was non-toxic to normal pancreatic ductal epithelial cells, and could specifically bind to and be internalized by MUC4/CEACAM6/CD44v6-expressing PDAC cells. With an r2 relaxivity of 104.2 mM−1 s−1, the IONPs-PEG-MCC triple scAbs nanocomposite could significantly shorten the MRI T2-weighted signal intensity both in vitro and in vivo. The IONPs-PEG-MCC triple scAbs nanocomposite also showed a favorable anti-pancreatic cancer effect. Conclusion: In the present study, the IONPs-PEG-MCC triple scAbs nanocomposite was firstly confirmed as a bi-functional nanocomposite in both MRI and treatment, providing its critical clinical transformation potential in PDAC detection and treatment.
研究领域:
纳米技术
·
医学影像
·
肿瘤学
·
生物医学工程
·
药学
✓ 已验证
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2020
·
IF 3.83
Jinyue Zhang,Mingshi Jin,Yong Il Park,Longyi Jin,Bo Quan
Manganese silicate nanoparticles have recently attracted great attention due to the effectiveness of T 1 -weighted magnetic resonance imaging (MRI) contrast agents (CAs) in the mildly acidic and high glutathione (GSH) conditions of the tumor microenvironment. Herein, ultra-small hollow manganese silicate (UHMS) nanoparticles were prepared via a facile, economical, and eco-friendly method based on a hydrothermal process using small silica nanoparticles as both the silicon source and templates. The nanoparticles showed remarkable biocompatibility and dispersion stability after modification with polyethylene glycol (PEG). Owing to their ultra-high longitudinal relaxation rate (r 1 ) at low pH values and high GSH levels, the [email protected] nanoparticles have performed an excellent pH/GSH-responsive T 1 -weighted MRI. Meanwhile, the nanoparticles showed insignificant cytotoxicity in vitro and in vivo. Therefore, the [email protected] nanoparticles show promise as T 1 -MRI CAs in the tumor microenvironment and may be used to assist in clinical cancer diagnosis.
研究领域:
纳米技术
·
材料科学
·
医学成像
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Qiuhui Hu,Yongzhao Su,Siying Ma,Peiying Wei,Chengbin He,Di Yang,Yue Qian,Youqing Shen,Xiaoxuan Zhou,Zhuxian Zhou,Hongjie Hu
Liver fibrosis is the critical stage in the development of chronic liver disease (CLD), from simple injury to irreversible cirrhosis. Timely detection and intervention of liver fibrosis are crucial for preventing CLD from progressing into a fatal condition. Herein, we developed iron oxide (Fe3O4) nanoparticles (IONPs) and ferulic acid (FA) coencapsulated poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs), followed by surface modification with cRGD peptides (cRGD-PLGA/IOFA) for integrin-targeted clinical magnetic resonance imaging (MRI)-traceable treatment of liver fibrosis. The cRGD peptide linked on the surface of the PLGA/IOFA NPs could specifically bind to the overexpressed integrin αvβ3 on activated hepatic stellate cells (HSCs) in the fibrotic liver, enabling the high-sensitive clinical MR imaging (3 T) and precise staging of liver fibrosis. The FA encapsulated in cRGD-PLGA/IOFA showed excellent efficacy in reducing oxidative stress and inhibiting the activation of HSCs through the transforming growth factor-β (TGF-β)/Smad pathway. Notably, the IONPs encapsulated in cRGD-PLGA/IOFA NPs could alleviate liver fibrosis by regulating hepatic macrophages through the NF-κB pathway, lowering the proportion of Ly6Chigh/CD86+, and degrading collagen fibers. The FA and IONPs in the cRGD-PLGA/IOFA produced a synergistic enhancement effect on collagen degradation, which was more effective than the IONPs treatment alone. This study demonstrates that cRGD-PLGA/IOFA NPs could effectively relieve liver fibrosis by acting on macrophages and HSCs and provide a new strategy for the clinical MRI-traceable treatment of liver fibrosis.
研究领域:
纳米技术
·
生物医学工程
·
药理学
·
医学影像
·
肝病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nano Research
·
2020
·Vol. 13
·期 1
·273-281
·
IF 8.183
Zhang Tao,Jiang Zhenqi,Chen Libin,Pan Chunshu,Sun Shan,Liu Chuang,Li Zihou,Ren Wenzhi,Wu Aiguo,Huang Pintong
As nanomedicine-based clinical strategies have continued to develop, the possibility of combining chemotherapy and singlet oxygen-dependent photodynamic therapy (PDT) to treat pancreatic cancer (PaC) has emerged as a viable therapeutic modality. The efficacy of such an approach, however, is likely to be constrained by the mechanisms of drug release and tumor oxygen levels. In the present study, we developed an Fe(III)-complexed porous coordination network (PCN) which we then used to encapsulate PTX (PCN-Fe(III)-PTX) nanoparticles (NPs) in order to treat PaC via a combination of chemotherapy and PDT. The resultant NPs were able to release drug in response to both laser irradiation and pH changes to promote drug accumulation within tumors. Furthermore, through a Fe(III)-based Fenton-like reaction these NPs were able to convert H 2 O 2 in the tumor site to O 2 , thereby regulating local hypoxic conditions and enhancing the efficacy of PDT approaches. Also these NPs were suitable for use as a T 1 -MRI weighted contrast agent, making them viable for monitoring therapeutic efficacy upon treatment. Our results in both cell line and animal models of PaC suggest that these NPs represent an ideal agent for mediating effective MRI-guided chemotherapy-PDT, giving them great promise for the clinical treatment of PaC.
研究领域:
肿瘤学
·
放射学
·
纳米医学
·
治疗诊断学
✓ 已验证
产品关联
DOI
高影响因子
Applied Materials Today
·
2024
·
IF 7.2
Qinqin Xu,Xiaohua Ban,Lingjie Yang,Yanrong Chen,Fang Zhang,Yu Wang,Peng Cao,Meng Yu,Xiaohui Duan
Chemodynamic therapy (CDT), which uses metal ions to covert endogenous hydrogen peroxide into highly reactive hydroxyl radicals, is an newly-developing strategy for cancer therapy. However, poor drug delivery efficiency and restricted catalytic performance hinder the therapeutic potential of CDT. Herein, we constructed an in situ injectable hydrogel incorporating Fe/Mn-polydopamine nanoparticles and the ferroptosis inducer piperazine erastin (PE) (FMPEG) for mediating tumor-specific magnetic resonance imaging-visualized photothermal therapy (PTT)/CDT. Upon exposure to near-infrared light irradiation, FMPEG treatment exerted a PTT effect, which further accelerated the drug release behavior from FMP nanoparticles in response to the acidic tumor microenvironment. The released metal ions and PE enhanced the CDT effect at the tumor site. Furthermore, a long-term antitumor effect was obtained via the immunostimulatory effect of CDT/PTT by producing tumor-associated antigens. These findings, along with the unique advantages of injectable hydrogels in immune activation, prove the great potential of FMPEG administration as an efficient strategy for the clinical prevention of distant tumor metastasis of breast cancer.
研究领域:
肿瘤学
·
医学影像
·
纳米技术
·
癌症治疗
·
免疫疗法
✓ 已验证
产品关联
DOI
高影响因子
Nano-Micro Letters
·
2020
·Vol. 12
·期 1
·1-14
·
IF 12.264
Fang Hanyi,Li Mengting,Liu Qingyao,Gai Yongkang,Yuan Lujie,Wang Sheng,Zhang Xiao,Ye Min,Zhang Yongxue,Gao Mingyuan,Hou Yi,Lan Xiaoli
A biomimetic nanoprobe was built with cancer cell membrane-coated and Gd 3+ -doped upconversion nanoparticles.
The nanoprobe could be applied to in vivo UCL/MRI/PET multimodality precise imaging and successfully differentiated MDA-MB-231 tumor models through in vivo tri-modality imaging, which may be used for breast cancer molecular classification.
研究领域:
纳米技术
·
生物医学工程
·
癌症研究
·
医学成像
✓ 已验证
DOI
PubMed
高影响因子
Journal of Clinical and Translational Hepatology
·
2022
·
IF 5.065
Feng-Yong Liu,Ming Shi,Xin Li,Hong-Jun Yuan,Xiao-Mei Tian,Yi-Mao Xia,Min Zhou,Fu-Sheng Wang
Background and Aims Stem cell transplantation is a potential treatment option for liver cirrhosis (LC). Accurately and noninvasively monitoring the distribution, migration, and prognosis of transplanted stem cells using imaging methods is important for in-depth study of the treatment mechanisms. Our study aimed to develop Au-Fe 3 O 4 silica nanoparticles (NPs) as tracking nanoplatforms for dual-modal stem cell imaging. Methods Au-Fe 3 O 4 silica NPs were synthesized by seed-mediated growth method and co-precipitation. The efficiency and cytotoxicity of the NPs-labeled bone marrow-derived mesenchymal stem cells (BM-MSCs) were evaluated by Cell Counting Kit-8 assays, ICP-MS, phenotypic characterization, and histological staining. The biodistribution of labeled BM-MSCs injected through different routes (the hepatic artery or tail vein) into rats with LC was detected by magnetic resonance imaging (MRI), photoacoustic imaging (PAI), and Prussian blue staining. Results Synthesized Au-Fe 3 O 4 silica NPs consisted of a core (star-shaped Au NPs) and an outside silica layer doped with Fe 3 O 4 NPs. After 24 h coincubation with 2.0 OD concentration of NPs, the viability of BM-MSCs was 77.91%±5.86% and the uptake of Au and Fe were (22.65±1.82) µg/mL and (234.03±11.47) µg/mL, respectively. The surface markers of labeled BM-MSCs unchanged significantly. Labeled BM-MSCs have osteogenic and adipogenic differentiation potential. Post injection in vivo , rat livers were hypointense on MRI and hyperintense on PAI. Prussian blue staining showed that more labeled BM-MSCs accumulated in the liver of the hepatic artery group. The severity of LC of the rats in the hepatic artery group was significantly alleviated. Conclusions Au-Fe 3 O 4 silica NPs were suitable MRI/PAI dual-modal imaging nanoplatforms for stem cell tracking in regenerative medicine. Transhepatic arterial infusion of BM-MSCs was the optimal route for the treatment of LC.
研究领域:
医学成像
·
再生医学
·
纳米技术
·
干细胞生物学
·
肝病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomaterials Science
·
2022
·Vol. 10
·期 5
·1317-1325
·
IF 7.59
Bengao Zhu,Xuefei Lv,Mengmeng Zhang,Huiying Wang,Senbin Chen,Jintao Zhu
Integrating magnetic resonance imaging (MRI)-targeted diagnosis with synergistic cascade treatments, such as chemo/chemodynamic therapy (CT/CDT), is highly desired for promoting the antitumor performance; however, the rational design of such “all-in-one” nanomedicine is still in its infancy. In this report, using MnO2 coated layered dihydroxide (LDH) as a carrier to load chemotherapy molecule 5-flurouracil (5-FU), a novel tumor microenvironment (TME) regulating nanodrug is formed: LDH/5-FU@MnO2. Combined guidance of CT/CDT and MRI is used to realize synergistic diagnosis and enhanced anti-tumor efficacy. MnO2 is converted into Mn2+ in the presence of reducing agent GSH, the in situ generated Mn2+, not only serves as the chemical fuel for the Fenton reaction, combining H2O2 depletion and ˙OH generation, but can also be used as a nuclear magnetic contrast agent for MRI. Moreover, the tumor acidic environment is able to trigger 5-FU release for initiating chemotherapy in the tumor zone. This “all-in-one” LDH/5-FU@MnO2 nanomedicine integrating multiple treatment modalities and magnetic resonance imaging properties, causes persistent modulation of the TME and exhibits effective antitumor theranostic performance. Such a sophisticated nanomedicine design not only achieves improved CT/CDT antitumor efficiency, but also realizes the activatable magnetic resonance imaging. This strategy combines the merits of each treatment, significantly enhancing the anticancer efficacy, and is anticipated to display promising potentials in the clinical translation plans.
研究领域:
纳米技术
·
癌症治疗
·
医学影像
·
药学科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）