CATALYSIS LETTERS
·
2023
·
IF 2.8
Su Zheng,Sun Yu,Zhang Qi,Wang Jia,Zhou Wenqi,Zhou Suyin,Zhang Jinlong,Liu Yongdi,Zhou Liang,Lei Juying
Photocatalytic cofactor regeneration often encounters the problem of insufficient stability of reduced nicotinamide adenine dinucleotide hydrogen (NADH) regeneration due to the influence of reactive oxygen species. Here, we obtained Pt-loaded CdS nanorods (CdS NRs) with a one-dimensional (1D) rod-like structure by simple hydrothermal and photo-deposition methods. The yield of NADH improved by 52.54% within 40 min. Superoxide radical (·O 2 − ) had been proven to be the main reactive species that leaded to unwanted oxidation after NADH regeneration. After loading Pt, the formation of Schottky junctions between Pt and CdS NRs promoted the separation and migration of photogenerated carriers, resulting in a tendency of ·O 2 − to shift toward hydroxyl radical (·OH). ·OH produces additional intermediates in the oxidation of NADH compared to ·O 2 − , which inhibits the unwanted oxidation of NADH. Meanwhile, the conduction band position of Pt/CdS shifted positively and the ability to reduce O 2 was weakened, thus the ·O 2 − in the system was reduced, which weakened the oxidation of NADH and significantly improved the stability of NADH regeneration. These studies provide a new reference for applying loaded metal catalysts to the stable regeneration of NADH. Graphical Abstract
研究领域:
催化
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材料科学
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光化学
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生物化学
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DOI
高影响因子
MICROCHIMICA ACTA
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2023
·Vol. 190
·期 7
·1-9
·
IF 5.7
Wang Wenxi,Ruan Shuyan,Su Zhixiong,Xu Peipei,Chen Yujia,Lin Zheng,Chen Jingbo,Lu Yudong
A novel S-CNF-based nanocomposite was created using sulfonated cellulose nanofiber (S-CNF) to enable the detection of NADH in serum by surface-enhanced Raman spectroscopy (SERS). The numerous hydroxyl and sulfonic acid groups on the S-CNF surface absorbed silver ions and converted them to silver seeds, which formed the load fulcrum. After adding a reducing agent, silver nanoparticles (Ag NPs) were firmly adhered to the S-CNF surface to form stable 1D “hot spots.” The S-CNF-Ag NP substrate demonstrated outstanding SERS performance, including good uniformity with an RSD of 6.88% and an enhancement factor (EF) of 1.23 × 10 7 . Owing to the anionic charge repulsion effect, the S-CNF-Ag NP substrate still maintains remarkable dispersion stability after 12 months of preservation. Finally, S-CNF-Ag NPs’ surface was modified with 4-mercaptophenol (4-MP), a special redox Raman signal molecule, to detect reduced nicotinamide adenine dinucleotide (NADH). The results showed that the detection limit (LOD) of NADH was 0.75 μM; a good linear relationship ( R 2 = 0.993) was established in the concentration range 10 −6 – 10 −2 M. The SERS nanoprobe enabled rapid detection of NADH in human serum without any complicated sample pretreatment and provides a new potential to detect biomarkers. Graphical
研究领域:
分析化学
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纳米技术
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生物医学工程
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生物传感器
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DOI
高影响因子
BIORESOURCE TECHNOLOGY
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2014
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IF 5.039
Yu-Cai He,Zhi-Cheng Tao,Xian Zhang,Zhen-Xing Yang,Jian-He Xu
An NADH-dependent reductase (CmCR) from Candida magnoliae was discovered by genome mining for carbonyl reductases. After CmCR was overexpressed in Escherichia coli BL21, a robust reductase-producing strain, recombinant E. coli CCZU-K14, was employed for the efficient synthesis of ethyl ( S )-4-chloro-3-hydroxybutanoate (( S )-CHBE) from the reduction of ethyl 4-chloro-3-oxobutanoate (COBE). After the optimization, the optimum reaction conditions were obtained. Notably, E. coli CCZU-K14 had broad substrate specificity in reducing both aliphatic and aromatic substrates, and excellent enantioselectivity of CCZU-K14 was observed for most of the tested substrates, resulting in chiral alcohols of over 99.9% ee . Moreover, COBE at a high concentration of (3000 mM) could be asymmetrically reduced to ( S )-CHBE in the high yield (>99.0%) and high enantiometric excess value (>99.9% ee ) after 14 h. Significantly, E. coli CCZU-K14 shows high potential in the industrial production of ( S )-CHBE and its derivatives (>99.9% ee ).
研究领域:
生物化学
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合成生物学
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酶工程
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DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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2014
·Vol. 173
·期 8
·2042-2053
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IF 1.687
He Yu-Cai,Yang Zhen-Xing,Zhang Dan-Ping,Tao Zhi-Cheng,Chen Chao,Chen Yi-Tong,Guo Fei,Xu Jian-He,Huang Lei,Chen Rui-Jie,Ma Xiao-Feng
The reductase (PgCR) from recombinant Escherichia coli CCZU-Y10 displayed high reductase activity and excellent stereoselectivity for the reduction of ethyl 4-chloro-3-oxobutanoate (COBE) into ethyl ( S )-4-chloro-3-hydroxybutanoate (( S )-CHBE). To efficiently synthesize ( S )-CHBE (>99 % enantiomeric excess (ee)), the highly stereoselective bioreduction of COBE into ( S )-CHBE with the whole cells of E. coli CCZU-Y10 was successfully demonstrated in a dibutyl phthalate-water biphasic system. The appropriate ratio of the organic phase to water phase was 1:1 ( v / v ). The optimum reaction temperature, reaction pH, cosubstrate, NAD + , and cell dosage of the biotransformation of 100 mM COBE in this biphasic system were 30 °C, 7.0, mannitol (2.5 mmol/mmol COBE), 0.1 μmol/(mmol COBE), and 0.1 g (wet weight)/mL, respectively. Moreover, COBE at a high concentration of (1,000 mM) could be asymmetrically reduced to ( S )-CHBE in a high yield (99.0 %) and high enantiometric excess value (>99 % ee). Significantly, E. coli CCZU-Y10 shows high potential in the industrial production of ( S )-CHBE (>99 % ee).
研究领域:
生物化学
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微生物学
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合成生物学
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PubMed
高影响因子
ACS Applied Nano Materials
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2025
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IF 5.5
Xintong Dong,Mengyuan Li,Xueyan Zhong,Mingqi Sun,Xiaoying Zhang,Ying Wang,Yao Wang,Ying Guo
Electrochemical biosensors based on nicotinamide adenine dinucleotide (NADH)-dependent dehydrogenases hold great potential in analytical applications. However, previous biosensors can lead to enzyme detachment and inactivation, thus limiting their applications. This study presents a novel sensing platform based on a three-dimensional MXene-graphene oxide sponge (3D MG). The nanoporous sponge was synthesized by polyethylene glycol (PEG) assisted cross-linking of MXene and small-sized graphene oxide (GO) nanosheets, thereby forming a high-strength porous conductive network and addressing the instability of enzyme-based biosensors. This sponge significantly enhances enzyme immobilization (reducing leaching by 62.27% compared to MXene films) and maintains enzymatic activity under extreme conditions. The 3D MG sensing platform was further fabricated into two biosensors by immobilizing NADH-dependent dehydrogenases (with glutamate dehydrogenase and formate dehydrogenase employed as model enzymes, respectively), using the (2,2′-bipyridyl) (pentamethylcyclopentadienyl)-rhodium complex ([Cp*Rh(bpy)Cl]+) as a mediator that enables the regeneration of bioactive NADH. The two biosensors exhibited low detection limits (0.332 μM for NH4+ and 0.188 mM for HCO3–), excellent reproducibility (relative standard deviation < 4%), and long-term stability (94.82% and 92.63% signal retention after 60 days). The fabrication of 3D MG with a robust nanoporous structure and an enzyme-friendly environment provides a strategy for designing long-life multifunctional enzyme-based biosensors.
研究领域:
生物传感器
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纳米材料
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电化学
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酶工程
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分析化学
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材料科学
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DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2022
·
IF 4.598
Minling Ding,Tongtong Hou,Huizhe Niu,Nan Zhang,Ping Guan,Xiaoling Hu
In this paper, a sensing platform for the electrocatalytic oxidation of nicotinamide adenine dinucleotide (NADH) was proposed. It was prepared by the electropolymerization of poly(thionine-methylene blue) (PTH-MB) in ternary nature deep eutectic solvent (NADES) on a glassy carbon electrode (GCE) that was modified with electrochemically reduced graphene oxide (ERG). The modified electrode was denoted as PTH-MB NADES -ERG/GCE. Cyclic voltammetry (CV), chronocoulometry (CC), electrochemical impedance spectroscopy (EIS), scanning electron microscope (SEM), Raman spectroscopy, and Ultra Violet-Visible Spectroscopy (UV–Vis) were used to characterize the preparation processes. The electrochemical performances of the electrode in NADH oxidation were studied via CV and current-time methods. The NADH oxidation overpotential was lower on PTH-MB NADES -ERG/GCE compared to that on GCE, ERG/GCE, and PTH-MB NADES /GCE. The PTH-MB NADES -ERG/GCE showed excellent electrocatalytic activity for NADH oxidation with a linear range of 0.51–3333.33 µM (R 2 = 0.9534) and a detection limit of 0.159 nM. The sensor was further tested for NADH determination in artificial urine samples, showing promising biomedical applications.
研究领域:
分析化学
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电化学
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材料科学
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生物医学工程
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Zongwen Wang,Xiating Li,Feng Zhang,Yu Gao,Jintian Cheng,FengFu Fu
It is an urgent need to develop simple and high-throughput methods for simultaneously screening and detecting multiple or groups of sulfonamides (SAs) in animal-derived foods since various SAs were alternately used in animal husbandry to avoid generating drug resistance. We herein developed a novel HCl-reduced nicotinamide adenine dinucleotide I (NADH)-ascorbic acid (AA)-mediated gold nanobipyramids (AuNBPs) growth system, which can precisely regulate the growth rate of AuNBPs, to generate two colorful and stable AA-corresponding multicolor signal channels with different sensitivities. Based on the HCl-NADH-AA-mediated AuNBP growth system, we further developed a dual-channel multicolor immunoassay for simultaneously realizing rapid screening and detection of 5 SAs (sulfamethazine, sulfamethoxydiazine, sulfisomidine, sulfamerazine, and sulfamonomethoxine) by using a paper-based analytical device for sensitively and stably reading out the signal and a broad-specificity anti-SAs antibody as a bio-receptor. The developed immunoassay has more color changes, a wider linear range, excellent specificity and stability, and two multicolor signal channels (L-channel and H-channel) with different sensitivities. The H-channel exhibited 7–8 SAs-corresponding color changes and can be used to detect 5 target SAs with a visual detection limit of 0.1–0.5 ng/mL and a spectrometry detection limit of 0.05–0.16 ng/mL. The L-channel exhibited 7–9 SAs-corresponding color changes and can be used to detect 5 target SAs with a visual detection limit of 2.0–6.0 ng/mL and a spectrometry detection limit of 0.40–1.47 ng/mL. The developed immunoassay was successfully used to simultaneously screen and detect low-concentration and high-concentration of target SAs in milk and fish muscle samples with a recovery of 85–110% and an RSD (n = 5) < 8%. The visual detection limit of our immunoassay is much lower than the maximum residue limit of total SAs in edible tissue. All above features make our immunoassay a promising assay for simultaneously realizing the rapid screening and quantitative determination of multiple SA residues in food by bare eye observation. It must be mentioned that our immunoassay may be expended as a general method for the simultaneous visual screening and detection of other drugs using the corresponding antibody as a recognition probe.
研究领域:
分析化学
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食品科学
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纳米生物学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
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2014
·Vol. 6
·期 18
·7496-7501
·
IF 1.938
Xiaoqian Ouyang,Yaping Ding,Xi Ba,Shuqing Gu
A poly-Ni(II)–curcumin (curcumin: 1,7-bis[4-hydroxy-3-methoxyphenyl]-1,6-heptadiene-3,5-dione) composite film modified electrode was prepared for amperometric determination of β-nicotinamide adenine dinucleotide (NADH). Scanning electron microscopy and Energy Dispersive X-ray spectroscopy were used to examine the morphology and interface property of the Ni(II)–curcumin film. Under optimum conditions, the amperometric detection of NADH provided a wide linear detection range (0.3–300 μM), high sensitivity (230.02 μA mM−1 cm−2) and low limit of detection (LOD = 0.18 μM, S/N = 3). Moreover, the modified electrode showed good stability and reproducibility, indicating that it can improve the analysis signal of NADH in a very convenient way.
研究领域:
电化学
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分析化学
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材料科学
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DOI
高影响因子
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2019
·Vol. 21
·期 44
·24572-24583
·
IF 3.567
Jiying Liang,Wenting Wei,Huiqin Yao,Keren Shi,Hongyun Liu
In this work, poly(N,N′-dimethylaminoethylmethacrylate-co-N-isopropylacrylamide) copolymer films were polymerized on the surface of Au electrodes with a facile one-step method, and Au nanoclusters (AuNCs) and tetraphenylethene (TPE) were synchronously embedded in the films, designated as P(DMA-co-NIPA)/AuNCs/TPE. Ferrocene dicarboxylic acid (FDA), an electroactive probe in solution displayed inverse pH- and SO42−-sensitive on–off cyclic voltammetric (CV) behaviors at the film electrodes. The electrocatalytic oxidation of nicotinamide adenine dinucleotide (NADH) mediated by FDA in solution could substantially amplify the CV response difference between the on and off states. Moreover, the two fluorescence emission (FL) signals from the TPE constituent at 450 nm and AuNCs component at 660 nm in the films also demonstrated SO42−- and pH-sensitive behaviors. Based on the aforementioned results, a 4-input/9-output biomolecular logic circuit was constructed with pH, Na2SO4, FDA and NADH as the inputs, and the CV signals and the FL responses at 450 and 660 nm at different levels as the outputs. Additionally, some functional non-Boolean devices were elaborately designed on an identical platform, including a 1-to-2 decoder, a 2-to-1 encoder, a 1-to-2 demultiplexer and different types of keypad locks. This work combines copolymer films, bioelectrocatalysis, and fluorescence together so that more complicated biocomputing systems could be established. This work may pave a new way to develop advanced and sophisticated biocomputing logic circuits and functional devices in the future.
研究领域:
生物化学
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纳米生物学
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分析化学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2022
·
IF 10.383
Weiwei Li,Chunhua Zhang,Ziman Zheng,Xiaoyu Zhang,Lin Zhang,Alexander Kuhn
Electrochemical regeneration of the reduced form of the nicotinamide adenine dinucleotide (NADH) cofactor catalyzed by immobilized [Rh(Cp*)(bpy)Cl]+ is a promising approach for the enzymatic synthesis of many valuable chemicals with NAD-dependent dehydrogenases. However, rational control of the efficiency is often limited by the irregular structure of the electrode/electrolyte interface and the accessibility of the molecular catalyst. Here, we propose an electrochemical system for NADH cofactor regeneration, based on highly ordered three- dimensional (3D) metal–organic framework (NU-1000) films. [Rh(Cp*)(bpy)Cl]+ is incorporated at the zirconium nodes of NU-1000 via solvent-assisted ligand incorporation (SALI), leading to a diffusion-controlled behavior, associated with an electron hopping mechanism. Varying the ratio of redox-active [Rh(Cp*)(bpy)Cl]+ and inactive postgrafting agents enables the elaboration of functional electrodes with tunable electrocatalytic activity for NADH regeneration. The exceptionally high faradic efficiency of 97%, associated with a very high turnover frequency (TOF) of ∼1400 h–1 for NADH regeneration, and the total turnover number (TTN) of over 20000 for the enzymatic conversion from pyruvate to l-lactate, when coupled with l-lactate dehydrogenases (LDH) as a model reaction, open up promising perspectives for employing these electrodes in various alternative bioelectrosynthesis approaches.
研究领域:
电化学
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催化
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生物电化学
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材料科学
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Yuanyuan Cai,Chongyang Liu,Jin Wang,Xuan Liu,Yujiao Zhang,Shuqing Yang,Lingxi Niu,Lingxing Zeng,Aihua Liu
Cu x O nanorods with excellent NADH peroxidase mimics were synthesized by a simple hydrothermal method . The catalytic oxidation of NADH to NAD cofactor strictly follows the enzymatic kinetics with high catalytic rate and strong affinity. The catalytic mechanism of Cu x O NRs was that in the presence of hydrogen peroxide, the catalytic oxidizing NADH to NAD + involving with O 2 .-. anion production, making it realistic to mutually convert between coenzymes . Considering that the mutual transformation of NADH/NAD cofactors plays an important role in biological function, combination of Cu x O NRs with alcohol dehydrogenase , a highly selective method for fluorimetric detection of ethanol was established. The as-proposed sensing platform is capable of dectecting alcohol with the limit of detection of 26.7 μM (S/N = 3) and applied in practical sample with satisfied accuracy and recovery. The as-developed regenerable NADH peroxidase mimics would also cast lights in biocatalysis , synthetic biology and bioenergy .
研究领域:
材料科学
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分析化学
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生物技术
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生物化学
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产品关联
DOI
PubMed
高影响因子
EXPERIMENTAL EYE RESEARCH
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2022
·
IF 3.77
Jun Chen,Yizhou Sun,Lei Chen,Yun Zhou
Diabetic retinopathy (DR) is a progressive vascular complication of diabetes mellitus (DM) and is related to retinal vascular abnormalities. NADH-Cytochrome B5 Reductase 2 (CBR2) has been implicated in angiogenesis , but the effect of CBR2 on angiogenesis and endothelial cell biological behavior in DR remains unclear. Here, we aimed to explore the effect of CBR2 on retinal vascular dysfunction under diabetic conditions. The histological analyses were performed to explore the effect of CBR2 on pathological change in streptozotocin (STZ)-induced diabetic rat retinas . The effect of CBR2 on endothelial cell function was explored by CCK-8, scratch wound, transwell, tube formation, and immunofluorescence assays in high glucose (HG)-stimulated human retinal microvascular endothelial cells (HRMECs). CBR2 expression was significantly downregulated in DM rat retinas and HG-stimulated HRMECs. Intravitreal injection of CBR2-expressing lentivirus under diabetic conditions reduced retinal angiogenesis, acellular capillary formation, and pericyte loss, along with decreased expression of hypoxia-inducible factor-1α (HIF-1α), cluster of differentiation 31 (CD31), and vascular endothelial growth factor A (VEGFA) in vivo . Moreover, CBR2 overexpression inhibited cell growth and tube formation and led to decreased expression of HIF-1α and VEGFA in HG-induced HRMECs. Interestingly, the repressive effects of CBR2 on cell proliferation , migration, and tube formation under HG conditions were strongly reversed when VEGFA was overexpressed. Overall, the key findings of our study suggested that CBR2 might alleviate retinal vascular dysfunction and abnormal endothelial proliferation during the process of DR by regulating VEGFA, providing a piece of potent evidence for DR therapy.
研究领域:
眼科学
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糖尿病
·
分子生物学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）