INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yalan Wu,Nan Zhao,Yiwei Jiang,Xiaodong Zheng,Ting Yu,Fujie Yan
Yeast-derived cell wall polysaccharides possess numerous biological activities, but their application in postharvest preservation is rarely reported. The aim of this research was to investigate the effects of Kluyveromyces marxianus soluble cell wall polysaccharide (SCWP) on preventing the infection of Penicillium expansum in pear fruit. The results showed that K. marxianus SCWP treatment could significantly improve the resistance of pear fruit to P. expansum , with respect to Saccharomyces cerevisiae -derived SCWP. Composition of both SCWPs was mannan with the main chains consisting of a → 6)-α-D-Manp-(1 → unit and the branch structure formed by → 2)-α-D-Manp-(1 except that K. marxianus SCWP took on a shorter side chain and a rougher surface than S. cerevisiae SCWP. In addition, mechanisms of K. marxianus SCWP on stimulating resistance response were associated with the apparent oxidative burst, increased gene expression and enzyme activity of antioxidant and defense systems in pear fruit. Our findings suggest that K. marxianus SCWP can be used as an innovative and promising candidate for preventing postharvest fungal decay and extending fruit shelf life.
研究领域:
农业科学
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植物病理学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
·
2026
·Vol. 23
·期 5
·e71299
·
IF 2.9
A-Long Zhang,Zheng-Biao Zou,Yan-Qing Guo,Wen-Si Su,Chun-Lan Xie,Li-Sheng Li,Cheng-Hai Gao,Xian-Wen Yang
Two new polyketides ( 1–2 ) were isolated from the deep-sea-derived fungus Penicillium citrinum W17, along with 39 known compounds ( 3 - 41 ). The structures were established by 1D and 2D NMR, HRESIMS data analysis. Compounds 10 , 35 , and 36 displayed potent inhibitory effects against RSL3-induced ferroptosis with EC 50 values of 2.7 µM, 4.9 µM, and 5.4 µM, respectively. Graphical From the deep-sea-derived fungus Penicillium citrinum W17, two new polyketides and 39 known compounds were isolated. Three compounds of 2,3,4-trimethyl-5,7-dihydroxy-2,3-dihydrobenzofuran ( 10 ), penicitrinone A ( 35 ), and penicitrinol B ( 36 ) significantly inhibit RSL3-induced ferroptosis with EC 50 values of 2.7 µM, 4.9 µM, and 5.4 µM, highlighting deep-sea fungi as promising sources of anti-ferroptosis agents.
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DOI
高影响因子
CHEMISTRY & BIODIVERSITY
·
2023
·Vol. 20
·期 9
·e202300991
·
IF 2.9
Hong-Yu Tan,Yu Yang,Rui Xu,Xue Zhao,Shuai-Ming Zhu,He-Xiang Gong,Zi-Lin Wang,Yue Lu,Hong-Wei Liu,Chang-Wei Li
A new polyaromatic metabolite, ent -herqueidiketal ( 1 ), and a new phenalenone derivative, epi -peniciherqueinone ( 2 ), along with twelve known compounds 3 – 14 , were isolated from the fungus Penicillium herquei YNJ-35, a symbiotic fungus of Pulveroboletus brunneopunctatus collected from Nangunhe Nature Reserve, Yunnan Province, China. The structures of 1 – 14 and the absolute configurations of 1 and 2 were determined by their spectroscopic data or by their single-crystal X-ray diffraction analysis or optical rotation values. Compound 1 showed strong antibacterial activity against Staphylococcus aureus (ATCC 29213) with minimum inhibitory concentration (MIC) of 8 μg/mL. In the cytotoxicity assays, compound 1 showed weak inhibitory activity against breast cancer MCF-7 and mice microglial BV2 cells with half maximal inhibitory concentration (IC 50 ) of 17.58 and 29.56 μM; compound 14 showed stronger cytotoxicity against BV2 and MCF-7 cells with IC 50 values of 6.57 and 10.26 μM. Graphical
研究领域:
真菌学
·
天然产物化学
·
药理学
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DOI
高影响因子
Marine Life Science & Technology
·
2026
·
IF 5.7
Zhang Yong,Guo Yan-Qing,Li You,Zou Zheng-Biao,Chen Zhe-Wen,Zhao Rong,Capon Robert J.,Wu Tai-Zong,Xie Chun-Lan,Yang Xian-Wen
A systematic chemical investigation of the deep-sea-derived fungus Penicillium limosum ZEN48 resulted in the isolation of four new indole-diketopiperazine alkaloids, limopiperazines A–D ( 1 – 4 ), alongside 16 known analogues ( 5 – 20 ). The structures of the new compounds were determined through comprehensive spectroscopic analysis, quantum chemical calculations, X-ray crystallography, and biogenetic considerations. Limopiperazine C ( 3 ) potently inhibited osteoclast differentiation and disrupted actin ring formation. Integrated RNA sequencing, RT-qPCR and molecular docking revealed that limopiperazine C exerts the anti-osteoclastogenic effect by modulating the ferroptosis signaling pathway via targeting heme oxygenase-1 (Hmox-1), positioning it as a promising lead compound for developing anti-osteoporotic agents.
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DOI
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2020
·
IF 4.303
Zehua Zhang,Shuhua Li,Dandan Sun,Ying Yang,Zhanhai Wei,Changlu Wang,Laifeng Lu
Penicillium digitatum acidifies the extracellular environments of fruit during decay development, this pH fluctuation decreased the efficacy of biological approaches under commercial conditions. Here, we explored the interaction between P. digitatum and an antagonistic yeast Rhodosporidium paludigenum to investigate potential mechanism behind the battle. P. digitatum was found in acidified citrus fruit tissue at pH 4.25 to 3.98 as mold infection progressed. Metabonomic data showed that gluconic, glucaric, and malic acids markedly increased upon infection at pathogen-fruit interaction sites. At concentrations > 1 × 10 6 cells mL −1 in liquid culture containing glucose or fructose instead of lactose, R. paludigenum could effectively control green mold. High concentrations of six different acids suppressed yeast growth even in the presence of sufficient glucose. Conversely, at ≤200 mmol L −1 , malic and gluconic acids promoted yeast growth in reverse. Cultivation of R. paludigenum with gluconic acid enhanced its ability to control green mold. This study provides new insights into the mechanisms by which extracellular acidification caused by acids produced by P. digitatum discriminates against competing microbes, thereby enhancing antagonistic yeast biocontrol of green mold in citrus.
研究领域:
植物病理学
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微生物学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Yiran Wang,Qiya Yang,Esa Abiso Godana,Yu Zhang,Hongyin Zhang
Penicillium expansum is a pathogen causing enormous postharvest losses of fruits, especially apples. In this study, we first investigated the morphological changes of P. expansum within apple wounds during infectious process by microscopic observation. We found that conidia swelled and secreted potential hydrophobin in 4 h, germinated in 8 h, and finally formed conidiophores in 36 h, a critical control time point to prevent the second contamination of spores. We then compared the transcript accumulation of P. expansum in apple tissues and liquid culture at 12 h. In total, 3168 and 1318 up-regulated and down-regulated genes were identified. Among them, genes regarding the biosynthesis of substances such as ergosterol, organic acid, cell wall degrading enzymes, and patulin were induced in expression. Pathways were activated, including autophagy, the mitogen-activated protein kinase, and pectin degradation. Our findings provide insights into the lifestyle and the mechanisms of P. expansum invading apple fruits.
研究领域:
植物病理学
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分子生物学
·
真菌生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF NATURAL PRODUCTS
·
2022
·
IF 4.803
Zhenzhen Zhang,Xueqian He,Xiaomin Zhang,Dehai Li,Guangwei Wu,Zhenzhen Liu,Chao Niu,Lanping Yang,Wenting Song,Zhanlin Li,Zhenhui Wang
Nineteen new talaroenamine derivatives, talaroenamines F1–F19 (1–19), were isolated from the Yellow River wetland derived Penicillium malacosphaerulum HPU-J01 by use of a one-pot/two-stage precursor-directed biosynthesis approach. During this approach, the initial precursor p-methylaniline was first used as a carrier to capture the biologically synthesized cyclohexanedione to produce talaroenamine F, and then the other aniline derivatives were employed to replace the p-methylaniline fragment of talaroenamine F to generate the final products. LC–MS analysis showed that only four compounds (2, 8, 10, and 12) could be produced by the traditional precursor-directed biosynthesis in which the aniline precursors were added simultaneously. Compound 14 was cytotoxic against the K562 cell line with an IC50 value of 2.2 μM. This work demonstrated the one-pot/two-stage precursor-directed biosynthesis could improve substrate acceptance leading to the production of diverse talaroenamines.
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DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2020
·Vol. 100
·期 10
·3812-3821
·
IF 2.614
Ou Chen,Lanhua Yi,Lili Deng,Changqing Ruan,Kaifang Zeng
BACKGROUND Penicillium digitatum is one of the most important pathogens causing citrus green mold, leading to significant economic losses. Traditionally, synthetic fungicides are used to control diseases. However, the side effects of fungicides should not be ignored. Thus, antagonistic yeasts were proposed to be safe and effective alternatives for managing diseases. Orchards are excellent sources of naturally occurring antagonists against pathogens. Therefore, in the present study, antagonistic yeasts obtained from orchards were screened, and the possible biocontrol mechanisms of the most promising yeast were investigated. RESULTS Seventy-eight isolates of yeasts (15 species of 10 genera) were obtained from citrus orchards. In in vitro assays, 16 strains showed antifungal activity against Pichia digitatum and 15 strains showed biocontrol potential against green mold on Olinda oranges. Pichia galeiformis (BAF03) exhibited the best antagonistic activity against P. digitatum during 6 days storage at 25 °C and a good antagonistic activity during 29 days at 4 °C. Pichia galeiformis (BAF03) could colonize and amplify quickly in wounded citrus. Scanning electron microscopy results showed that the citrus wound was colonised by the yeast. A total of eight volatile organic compounds (VOCs) were identified by gas chromatography-mass spectrometry The VOCs produced by P. galeiformis (BAF03) efficiently inhibited P. digitatum . CONCLUSION Pichia galeiformis (BAF03) isolated from a citrus orchard showed potential to control postharvest green mold of citrus. The possible mechanisms of action likely include competition for space and nutrients as well as production of VOCs.
研究领域:
植物病理学
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微生物学
·
农业科学
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DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2025
·
IF 4
Duo Lai,Delin Wang,Jian Qin,Qingli Zhuang,Xuehua Shao,Weiqiang Xiao
Emblica blue mold, a fungal disease caused by Penicillium choerospondiatis , poses a major threat to the industry of Phyllanthus emblica . Currently, resistance to chemical fungicides has been a challenge to its effectiveness on this disease. To explore an effective botanical fungicide as alternatives for chemical fungicides to inhibit P. choerospondiatis , 10 botanical fungicides were selected for antifungal activity test against P. choerospondiatis mycelial growth. Results showed that physcion was the most effective one with a 50 % effective concentration (EC 50 ) of 3.30 mg/L. Furthermore, physcion had a strong inhibition activity to spore germination and pathogenicity of P. choerospondiatis , both in vitro and in vivo . Its antifungal mechanisms investigations indicated that physcion could alter the mycelium morphology, disrupt cell membrane integrity, increase cell membrane permeability, reduce ergosterol content, and induce excessive reactive oxygen species (ROS). Moreover, transcriptome analysis revealed that physcion might interfere with ergosterol biosynthesis and oxidoreductase activity, leading to significant cell membrane dysfunction and oxidative stress in P. choerospondiatis . This study lays the groundwork for elucidating how physcion harms the pathogen and provides promising insights into eco-friendly alternatives for controlling this postharvest disease in P. choerospondiatis .
研究领域:
植物病理学
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真菌学
·
采后病害防治
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天然产物化学
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真菌生理学
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转录组学
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产品关联
DOI
PubMed
高影响因子
FUNGAL GENETICS AND BIOLOGY
·
2022
·
IF 3.883
Jiazhen Qin,Jianwen Teng,Zhongyu Li,Ning Xia,Baoyao Wei,Li Huang
Similar to Pu-erh tea, Liupao tea is a post-fermented tea that is produced through natural fermentation by microorganisms. Penicillium citrinum is involved in multiple production processes of Liupao tea that can produce citrinin, a secondary metabolite with renal toxicity; however, the effect of P. citrinum on the quality of Liupao tea has not been investigated yet. Citrinin production is regulated by approximately 16 biosynthesis genes. However, little is known about the genetic background of citrinin in the complex Liupao tea system. In the present study, we cultured P. citrinum on potato dextrose agar and Liupao tea powder media and analyzed the changes of its nutritional components in Liupao tea. We selected six citrinin biosynthesis genes identified in Monascus exhibiting homology and high sequence similarity to those in P. citrinum and further analyzed the expression of citrinin biosynthesis genes in Liupao tea and the changes in citrinin yield. The results showed that the changes in nutritional components of Liupao tea were closely related to the growth and metabolism of P. citrinum and the quality of the tea. Decreases in the contents of soluble sugars (from 10.29% to 9.58%), soluble pectins (from 3.71% to 3.13%), free amino acids (from 3.84% to 3.14%), and tea polyphenols (from 22.84% to 18.78%) were noted. The Spearman’s correlation analysis indicated that P. citrinum growth can improve the tea quality to some extent. Quantitative real-time PCR demonstrated that ctnA gene was a positive regulator of citrinin production regardless of the culture medium used. ctnA and orf5 expressions greatly influenced the metabolism of citrinin by P. citrinum in Liupao tea. In conclusion, the citrinin biosynthesis genes, ctnA and orf5 , may be the promising targets for developing strategies to control P. citrinum infection and citrinin biosynthesis in Liupao tea.
研究领域:
食品科学
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微生物学
·
分子生物学
·
制茶技术
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DOI
PubMed
高影响因子
Frontiers in Nutrition
·
2021
·
IF 6.576
Yang Ruopeng,Chen Xiu,Huang Qiang,Chen Chuying,Rengasamy Kannan R. R.,Chen Jinyin,Wan Chunpeng (Craig)
Penicillium digitatum is the most severe pathogen that infects citrus fruits during storage. It can cause fruit rot and bring significant economic losses. The continuous use of fungicides has resulted in the emergence of drug-resistant strains. Consequently, there is a need to develop naturally and efficiently antifungal fungicides. Natural antimicrobial agents such as clove oil, cinnamon oil, and thyme oil can be extracted from different plant parts. They exhibited broad-spectrum antimicrobial properties and have great potential in the food industry. Here, we exploit a novel cinnamaldehyde (CA), eugenol (EUG), or carvacrol (CAR) combination antifungal therapy and formulate it into nanoemulsion form to overcome lower solubility and instability of essential oil. In this study, the antifungal activity evaluation and transcriptional profile of Penicillium digitatum exposed to compound nanoemulsion were evaluated. Results showed that compound nanoemulsion had a striking inhibitory effect on P. digitatum in a dose-dependent manner. According to RNA-seq analysis, there were 2,169 differentially expressed genes (DEGs) between control and nanoemulsion-treated samples, including 1,028 downregulated and 1,141 upregulated genes. Gene Ontology (GO) analysis indicated that the DEGs were mainly involved in intracellular organelle parts of cell component: cellular respiration, proton transmembrane transport of biological process, and guanyl nucleotide-binding molecular function. KEGG analysis revealed that metabolic pathway, biosynthesis of secondary metabolites, and glyoxylate and dicarboxylate metabolism were the most highly enriched pathways for these DEGs. Taken together, we can conclude the promising antifungal activity of nanoemulsion with multiple action sites against P. digitatum. These outcomes would deepen our knowledge of the inhibitory mechanism from molecular aspects and exploit naturally, efficiently, and harmlessly antifungal agents in the citrus postharvest industry.
研究领域:
微生物学
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分子生物学
·
植物病理学
·
植病学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2023
·
IF 4.7
Duo Lai,Delin Wang,Xuehua Shao,Jian Qin,Qingli Zhuang,Hanhong Xu,Weiqiang Xiao
Blue mold induced by Penicillium choerospondiatis is a primary cause of growth and postharvest losses in the fruit of Phyllanthus emblica . There is an urgent need to explore novel and safe fungicides to control this disease. Here, we demonstrated osthole, a natural coumarin compound isolated from Cnidium monnieri , exhibited a strong inhibitory effect on mycelia growth, conidial germination rate and germ tube length of P. choerospondiatis , and effectively suppressed the blue mold development in postharvest fruit of P. emblica . The median effective concentration of osthole was 9.86 mg/L. Osthole treatment resulted in cellular structural disruption, reactive oxygen species (ROS) accumulation, and induced autophagic vacuoles containing cytoplasmic components in fungal cells. Transcriptome analysis revealed that osthole treatment led to the differentially expressed genes mainly enriched in the cell wall synthesis, TCA cycle, glycolysis/ gluconeogenesis , oxidative phosphorylation . Moreover, osthole treatment led to increase genes expression involved in peroxisome , autophagy and endocytosis . Particularly, the autophagy pathway related genes ( PcATG1 , PcATG3 , PcATG15 , PcATG27 , PcYPT7 and PcSEC18 ) were prominently up-regulated by osthole. Summarily, these results revealed the potential antifungal mechanism of osthole against P. choerospondiatis . Osthole has potentials to develop as a natural antifungal agent for controlling blue mold disease in postharvest fruits.
研究领域:
植物病理学
·
分子生物学
·
药理学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）