MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 8
·1-10
·
IF 5.479
Hu Yunxia,Su Luyao,Wang Sui,Guo Zhiyong,Hu Yufang,Xie Hongzhen
A method is described for combined magnetic solid phase microextraction and electrochemiluminescent (ECL) detection of the antibiotic tetracycline. A nanocomposite of type Fe 3 O 4 @SiO 2 @ZnO was used as the sorbent. Tetracycline has a strong affinity for Zn(II) ion and therefore is well extracted by this sorbent. The loaded sorbent can be magnetically removed. The extraction efficiency at a 1.0 μM tetracycline concentration is around 88%. Detection is based on the use of carbon nanodots that were prepared form urea and ethylenediaminetetraacetic acid as raw materials. The electrochemical probe Ru(bpy) 3 2+ was added to generate double ECL when scanning the potential between −3.5 and + 2 V. The two ECL signals decreased with the increase of tetracycline concentration. Under optimized experimental conditions, the ratio of the two signals is linearly related to the logarithm of the tetracycline concentration in the range from 1.0 nM to 0.1 mM, with a 0.47 nM detection limit. The method was successfully applied to the determination of tetracycline in spiked milk. It exhibited good sensitivity, selectivity and accuracy due to ratiometric read-out and prior preconcentration of analyte.
Tetracycline (TC) has a strong affinity for Zn(II) ion and is well extracted by the Fe 3 O 4 @SiO 2 @ZnO nanocomposite. Ru(bpy) 3 2+ can generate double electrochemiluminescence signals based on the use of carbon nanodots (C-dots) as coreactant. The two signals decrease with the increase of tetracycline concentration. [Ru(bpy) 3 2+ ]* stands for excited state Ru(bpy) 2 2+ .
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TALANTA
·
2024
·
IF 5.6
Shuning Yang,Li Tian,Weiwei Fu,Huiling Li,Chao Li,Yujia Song,Ruidan Li,Yanjia Guo,Lun Zhao
The novel TiO 2 and Ni-MOF materials were synthesized and utilized for the detection of permethrin (PET). A highly sensitive solid-state electrochemiluminescence (ECL) sensor was developed based on Ni-MOF@Ru(bpy) 3 2+ and Au NPs@TiO 2 . In this sensing platform, Ru(bpy) 3 2+ -Tripropyl Amine (TPrA) was used as a luminescent signal, Ni-MOF acted as a carrier to carry more luminescent reagents Ru(bpy) 3 2+ . Au NPs acted as promoters facilitated electron transport and TiO 2 could further enhance the luminescence intensity of the system by synergistical interaction with Au NPs. The possible mechanisms of signal amplification were investigated. The ECL intensity decreased significantly with increasing PET concentration, enabling the determination of PET amount through the observation of the change in ECL signal intensity (ΔI). Under optimal experimental conditions, the linear range of PET concentration from 1.0 × 10 −11 mol L −1 to 1.0 × 10 −6 mol L −1 , with a detection limit of 3.3 × 10 −12 mol L −1 (3S/N). This method was successfully applied to determine PET in various vegetable samples.
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MOLECULES
·
2025
·Vol. 30
·期 15
·3130
·
IF 4.6
Jun Li,Jianlan Deng,Qian Tao,Chenyu Yan,Yuxuan Liu,Jianxiao Yang,Zhong Cao
Luminescent metal–organic frameworks (MOFs) are used for the detection of organophosphorus pesticides (OPs) due to their large surface area and pore volume as well as their special optical properties. However, most self-luminescent MOFs are not only complex to synthesize and unstable in water but also feature a “turn-off” sensing system, which has highly restricted their practical applications in OP detection. Herein, a “turn-on” fluorescence sensor based on the guest-induced luminescence MOF Ru(bpy)32+@UiO-66 was constructed, which realized the sensitive detection of OPs through a dual-enzyme system for the first time. Compared with self-luminescent MOFs, Ru(bpy)32+@UiO-66 was not only more easily synthesized but also had higher chemical and photostability in water. In this strategy, by means of the hydrolysis of AChE and ChOx, H2O2will be produced, which can oxidize Fe2+to Fe3+, thereby quenching the fluorescence of Ru(bpy)32+@UiO-66. In the presence of OPs, the activity of AChE can be inhibited, resulting in the inability to generate H2O2and Fe3+, which will turn on the fluorescence signal of Ru(bpy)32+@UiO-66. As a result, the Ru(bpy)32+@UiO-66 sensing system not only had high sensitivity for OPs detection but also possessed a satisfactory detection recovery rate for parathion-methyl in real samples, which provides a new approach for OP detection in food safety as well as environmental monitoring.
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ACS Applied Nano Materials
·
2026
·
IF 5.8
Weiwei Zhang,Dongqin Ye,Jingcheng Zheng,Yuwu Chi
Determination of the solvent polarity is important across various scientific and industrial fields. Classic empirical parameters for evaluating solvent polarity, such as Rohrschneider–Snyder parameters (P′), Reichardt’s ET(30), Kamlet–Taft parameters, dielectric constant (ε) measurement, and Hansen solubility parameters (HSPs) often suffer from complicated instrumentation and measurement operation, which seriously limits their applications in real-time and fast determination and selection of unknown solvent polarity. In this work, we utilize recently emerging perovskite quantum dots (PQDs) with extremely high fluorescence quantum yield, high sensitivity to solvent polarity, and facile preparation and surface engineering to to develop a visualized and disposable solvent polarity sensor based on dual-fluorescent PQDs@SiO2/Ru(bpy)32+@SiO2 (PQDs@SiO2/Ru(II)@SiO2) nanocomposites. Among the nanocomposites, the components of PQDs@SiO2 are stable in nonpolar solvents but not stable in polar solvents due to degradation. Meanwhile, the component Ru(bpy)32+ is stable in both polar and nonpolar solvents, giving a constant red background fluorescence. Therefore, when inserting the glass slide loaded with PQDs@SiO2/Ru(II)@SiO2 nanocomposites into a nonpolar solvent, it retainss green light emission since the fluorescence quantum yield of PQDs is much larger than that of Ru(bpy)32+. In contrast, when the glass slide contacts with polar solvents, its fluorescence color will change from green to yellow, then orange, and finally to red due to the instability of PQDs in this case. The complete color change time from initial green to final red (tccc) was proposed as a new type of solvent polarity parameter. This work not only provides an empirical parameter for evaluating solvent polarity but also opens up an avenue for detecting solvent polarity using functional nanomaterial.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Ying Yu,Xiaodan Wang,Mingyang Li,Dengyong Liu
5′-Guanosine monophosphate (5′-GMP) is one main source of freshness in broths. Herein, an electrochemical platform based on a novel ternary nanocomposite glassy carbon electrode modified with advantageously-united gold nanoparticles, 2,2′-bipyridine hydrated ruthenium (Ru(bpy) 2 Cl 2 ) and sulfonated multi-walled carbon nanotubes (SMWCNTs)was prepared and used to detect 5′-GMP. After conditions optimization, the best performance of the electrochemical sensor was found in acidic media, including high specificity, sensitivity and selectivity. The electrochemical sensor exhibited a wide linear range under the optimal conditions. The enhanced sensitivity of this sensor was attributed to the Ru(bpy) 2 Cl 2 and functionalized SMWCNTs that provided high electrical conductivity and electrocatalytic properties during electrochemical reaction . Precise analysis of 5′-GMP in actual broth samples showed satisfactory recovery. Thus, the sensor can be used in the market and food enterprises.
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MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Xueyuan Li,Xuezhong Du
Surface enhanced electrochemiluminescence (SEECL) emission of the Ru(bpy) 3 2+ (bpy = 2,2′-bipyridyl)/tripropylamine (TPA) system by [email protected] 2 core/shell nanoparticles (NPs) was achieved owing to the electromagnetic field enhancement induced by localized surface plasmon resonance (LSPR) of internal Au cores. The Ru(bpy) 3 2+ /TPA system in electrolyte solution showed a maximum ECL enhancement when a glassy carbon electrode (GCE) was modified with [email protected] 2 NPs ( [email protected] 2 /GCE; Au core, ca. 68 nm; silica shell, 12 nm). The ECL emission of the system in the case of [email protected] 2 /GCE was 29 fold stronger than the ECL emission in the case of bare GCE, ca. 39 fold stronger than the ECL emission in the case of hollow silica NP-modified GCE, and 100 fold stronger than the ECL emission in the case of AuNP-modified GCE. To the best of our knowledge, the respective folds of ECL enhancement in the Ru(bpy) 3 2+ /TPA system by the LSPR-induced electromagnetic field of AuNPs are the largest reported to date. The ECL emission of TPA •+ radical cations was detected for the first time taking the advantage of the electromagnetic field enhancement. A SEECL platform based on the [email protected] 2 /GCE in the Ru(bpy) 3 2+ /TPA system was constructed for the detection of dopamine (DA). This is the first example that the AuNP-enhanced ECL emission was quenched by DA for the selective detection of DA, and a wide linear range of 0.01–600 μM directly relevant to DA concentration was obtained with a low detection limit of 0.004 μM and high sensitivity and accuracy. The mechanism that the ECL emission of the Ru(bpy) 3 2+ /TPA system was quenched by DA for the detection of DA was clearly elaborated. The level of DA in human serum was determined using the SEECL platform.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2013
·
IF 3.535
Meng Li,Hongmei Yang,Chao Ma,Yan Zhang,Shenguang Ge,Jinghua Yu,Mei Yan
In this paper, a novel and sensitive signal-off electrochemiluminescence (ECL) aptasensor was proposed for the detection of adenosine triphosphate (ATP) on a nanoporous gold (NPG) modified glassy carbon electrode. The NPG was easily synthesized by a selective dissolution of silver from silver/gold alloy in nitric acid . The ECL aptasensor consisted of two main elements: an ECL substrate, made by modifying the amino group functionalized Ru(bpy) 3 2+ -doped silica (Ru-silica), and an ECL intensity switch, which contains two probes, split from one aptamer. The first probe (ssDNA1) was functionalized with a thiol group at one end and attached to Ru-silica through the linking agent of N-succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate. The other one (ssDNA2) is ferrocene-labeled probe, which was functionalized with ferrocene tag at the amino group functionalized end. We demonstrated that the ssDNA1/ATP/ssDNA2 sandwich composites layer could be formed in the presence of ATP, and lead to a quenching of Ru(bpy) 3 2+ . However, only a slight decrease could be observed in the absence of ATP. Under optimal conditions, the proposed biosensor provided a wide linear response range over 0.1 pmol L −1 –10.0 nmol L −1 with a detection limit of 0.03 pmol L −1 .
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SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Hongkun Li,Qianqian Cai,Yangyang Guan,Zhikang Li,Guifen Jie,Hong Zhou
A multimode sensing and imaging platform based on surface modification strategy of Ru(bpy) 3 2+ doped SiO 2 nanoparticles (RuSiO 2 NPs) was constructed for versatile detection of glutathione (GSH). RuSiO 2 NPs as the luminophore models had outstanding fluorescence (FL) and electrochemiluminescence (ECL) signals. Furthermore, its FL signal could be captured by electrophoresis analyzer, and the ECL signal could be imaged with mobile phone to achieve visualization. So, a unique and convenient multi-mode sensing and imaging platform could be developed based on RuSiO 2 NPs. SiO 2 NPs could realize enrichment of Ru(bpy) 3 2+ based on the nanoconfined effect to achieve signal amplification, and its surface was rich with abundant hydroxyl functional groups, which offered a channel for surface modification. Sulfhydryl modification on the RuSiO 2 NPs was realized by using silane coupling agent, and KMnO 4 was used to in situ generate MnO 2 shell on the surface of RuSiO 2 NPs for quenching its ECL, FL, and colorimetric (CL) signals based on resonance energy transfer (RET). Target GSH could reduce MnO 2 shell to realize recovery of all signals, thus achieving the label-free multimode detection of GSH. Therefore, this work developed a multimode sensing and imaging platform without signal labels or layer-by-layer assembly of electrodes, which had the advantages of simple steps, low cost, and high detection accuracy, showing important application potential in the field of bioanalysis.
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 8
·1-12
·
IF 5.3
Xiao Rui,Wu Di,Liu Chun,Wu Tong,Ma Lingyan,Tan Long,Qi Wenjing
A novel electrochemiluminescence (ECL) enhanced method for sensitive, selective and accurate ECL detection of ascorbic acid (AA) is developed using tris (2,2′-bipyridine) ruthenium (II) (Ru(bpy) 3 2+ ) embedded into zirconium-based metal-organic frameworks (Ru(bpy) 3 2+ @UiO-67 MOFs). AA is oxidized by cerium ion (Ce 4+ ) into dehydroascorbic acid (DHA) and hydrolyzed into 2,3-diketogulonic acid (DKG). The oxidation product DKG has α-keto acid structure and can form acyl radical, which enhances the ECL of Ru(bpy) 3 2+ @UiO-67 MOFs nearly 220 times. Therefore, AA is used as an efficient ECL co-reactant of Ru(bpy) 3 2+ @UiO-67 MOFs and applied in highly sensitive ECL enhanced detection of AA from 0.1 μM to 100 μM with a limit of detection of 29 nM. More importantly, this method also has high selectivity in AA detection toward other common ions, substances with similar structure to AA and some reducing substances owing to specific α-keto acid structure of the oxidation product DKG of AA. It achieves ECL enhanced detection of AA in tablets, chewable tablets and beverages. Graphical
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ELECTROANALYSIS
·
2023
·Vol. 35
·期 9
·e202300052
·
IF 3
Qiaoyu Ding,Xi Bai,Jiali Ju,Jiajun Shi,Guixin Li
A sensitive electrochemiluminescence (ECL) biosensor for the specific DNA sequence of hepatitis C virus (HCV) was developed based on the efficient quenching effect of the ferrocene cluster functionalized gold nanoparticles (Fc@AuNPs) on the ECL of electrodeposited silica@Ru(bpy) 3 2+ -chitosan-graphene oxide nanocomposite (SiO 2 @Ru−CS−GO). Graphene oxide (GO) can accelerate electron transfer rate, thus improving the ECL of Ru(bpy) 3 2+ on electrode surface. The molecular beacons (MB) was fixed to SiO 2 @Ru−CS−GO by glutaraldehyde (GA) using the Schiff reaction between amino groups of chitosan (CS) and MB. The ECL of SiO 2 @Ru−CS−GO was depressed greatly by the Fc@AuNPs labelled at the end of MB, then, a stronger ECL was observed when the distance between Fc@AuNPs and SiO 2 @Ru−CS−GO increased after the hybridization of target DNA with MB. Under optimum conditions, the restored ECL intensity increased linearly with the target DNA concentration in the range of 1.0×10 −16 ∼1.0×10 −10 mol ⋅ L −1 , and the limit of detection (LOD) is 1.4×10 −17 mol ⋅ L −1 . The proposed method exhibits acceptable stability and reproducibility. In general, the constructed HCV biosensor can be used for the sensitive detection of HCV in human serum, suggesting potential application prospects in bioanalysis.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2020
·
IF 3.807
Zhimin Liu,Hao Wu,Xianguo Ge,Haijun Zhan,Leqian Hu
Graphitic carbon nitride quantum dots (g-CNQDs) with special structures have exhibited fascinating advantages over the common QDs. In this work, we report a novel Ru(bpy) 3 2+ /g-CNQDs electrochemiluminescence (ECL) system, and the effect of g-CNQDs on the ECL signal of Ru(bpy) 3 2+ was discussed. It was found that g-CNQDs containing in proposed ECL system played an important coreactant role in ECL signal enhancement of Ru(bpy) 3 2+ , which was proved by electrochemical, ultraviolet, and ECL experiments. Based on the quenching effect of catechol on the ECL signal of Ru(bpy) 3 2+ /g-CNQDs, a quenching-based ECL system was designed for catechol determination. The ECL sensing system demonstrated high sensitivity to catechol, with a linear range from 10 nM to 2 mM and a detection limit of 2.5 nM (S/N = 3). The sensor was adopted for catechol analysis in tea samples with satisfactory results.
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Sensors and Actuators Reports
·
2025
·
IF 7.6
Yanli Sun,Hongying Li,Tao Wang,Kai Zhang,Xueliang Wang
MicroRNA-17 (miR-17) is a critical biomarker linked to various cancers and diseases, underscoring the need for sensitive and selective detection methods. Herein, an ultra-sensitive electrochemiluminescent (ECL) sensor for miR-17 detection utilizing MOF-derived porous CuFe 2 O 4 nanospheres and CRISPR/Cas13a trans-cleavage-triggered hybridization chain reaction (HCR) amplification was presented. The porous CuFe 2 O 4 nanospheres possessed a high surface area and excellent catalytic activity, significantly enhancing ECL emission. To the best of our knowledge, this is the first report demonstrating that MOF-derived CuFe 2 O 4 can effectively enhance the electrochemiluminescence of Ru(bpy) 3 2 ⁺ for miR-17 detection. The CRISPR/Cas13a system provided miR-17 recognition and trans-cleavage activity, triggering HCR for signal amplification. Under optimized conditions, the biosensor exhibited a wide linear detection range from 20 aM to 5000 aM, with a detection limit of 9.23 aM. The system discriminated miR-17 from closely related miRNAs with negligible cross-reactivity, which demonstrated its outstanding selectivity. Practical applicability was confirmed by successful quantification of miR-17 in diluted human serum which achieved recovery rates between 95.2% and 104.8%. The results validated the biosensor’s potential for early disease diagnosis and clinical monitoring of miR-17-associated disorders.
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