TALANTA
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2025
·
IF 6.1
Yang Xu,Cuixia Guo,Yuxuan Yang,Chongqi Zhou,Lizhong Zhang,Han Li,Zishuo Song,Bei Wang,Yonghong He,Ke Cao
Protein-polyphenol interactions are crucial in food chemistry, yet current detection methods have limitations. This study innovatively uses optical weak measurement. Key variables like central wavelength shifts were measured in systems with chlorogenic acid, tea polyphenols, and various proteins. A linear relationship between polyphenol concentration and wavelength shift was found, and a new calculation strategy for binding constants and sites was developed, showing consistent trends with fluorescence quenching. The method is label-free and applicable to non-immobilized biomolecules, offering cost-effective and accurate detection. However, it's sensitive to solution fluctuations and complex samples. Future research should optimize the device and detection techniques to improve stability and capture interaction dynamics. This study provides a valuable tool for food chemistry research.
研究领域:
食品化学
·
生物物理学
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2025
·
IF 5.7
Linhua Li,Qingyin Li,Chunle Zhang,Zhengjiang Cao,Chang Liu,Rifang Luo,Yunbing Wang,Xiaoxi Zeng,Ping Fu
Chronic wounds are a common and serious complication. Abnormal microenvironments such as excessive oxidative stress, chronic inflammation, bacterial infection, and cellular dysfunction severely inhibit the wound healing process, leading to increased rates of amputation and mortality in diabetic patients. In this study, a novel polyphenol–activated protein coating (PDA/HK@LZM) was successfully constructed via the adsorption of lysozyme on the surface of a polyphenol nanocomposite comprising dopamine and honokiol. The coating effectively maintains the integrity of secondary and tertiary protein structures and preserves up to 91% of the lysozyme activity. Moreover, the PDA/HK@LZM coating achieves the spatiotemporal regulation of the chronic wound microenvironment. In the initial stage, lysozyme dominates the immune response by enhancing macrophage phagocytosis and modulating inflammatory factors, while honokiol synergizes with free radical scavenging and antimicrobial activity; all three synergistically enhance the wound self-cleaning function. In the repair stage, exposed honokiol regulates inflammation, polarizing macrophages from the pro-inflammatory M1-type to the anti-inflammatory M2-type and accelerating wound repair. Moreover, the release of honokiol into the microenvironment prevents the formation of vascular microthrombi, facilitating the delivery of nutrients and oxygen. Full-thickness skin wound experiments confirmed the effectiveness of the PDA/HK@LZM coating dressing in promoting rapid healing of chronic wounds. This study introduces a novel strategy for constructing polyphenol–activated protein coatings with spatiotemporal modulation wound microenvironment, opening new possibilities for the efficient treatment of chronic wounds.
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PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Qi Xie,Wei Chen,Zijian Huang,Yuan Gao,Haoyu Zhang,Zhanhao Zhou,Samira Batur,Chuansheng Fu,Jiao Zhang,Conglian Yang,Junjun Liu,Li Kong,Zhiping Zhang
Despite the nascent stage of research into protocells and prototissues, their substantial potential has increasingly positioned them as a focal point in recent years. The bottom-up reconstruction of life systems is gradually garnering widespread attention. In this study, a type of protocells and corresponding prototissues based on polyphenol coacervate droplets were developed via hydrogen bond interactions. The droplets exhibited properties reminiscent of cytoplasm, enriching a wide range of substances. Upon further modification with lipid bilayers, the protocells not only achieved a structural resemblance to natural cells, but also effectively maintained the stability. Prototissues formed by the orderly aggregation of protocells can not only encapsulate living cells, but also maintain the structural integrity and function of both artificial and living cells. At the macroscopic level, the prototissues were injectable, customizable in shape, bioadhesive, and capable of long-term retention in vivo. Moreover, the prototissues showed no obvious inflammatory responses. Polyphenol-based protocells and tissues with unique characteristics may offer new insights for research and applications in organoids, transplantation, and medical devices.
研究领域:
合成生物学
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生物物理学
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材料科学
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生物工程
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细胞生物学
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组织工程
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高影响因子
ADVANCED MATERIALS
·
2025
·
IF 26.8
Jun Cheng,Yao Shen,Yulu Gu,Tongyue Xiang,Hui Shen,Yi Wang,Zhenyang Hu,Zhen Zheng,Zhilong Yu,Qin Wu,Yinghui Wang,Tiancong Zhao,Yunfei Xie
A low-cost, high-precision, and secure real-time system for monitoring food freshness can significantly improve spoilage issues, yet traditional colorimetric sensor arrays often suffer from chemical dyes’ high toxicity and limited color changes. Here, a metal-polyphenol network colorimetric sensor array (MPN-CSA) is built for detecting total volatile base nitrogen (TVB-N) markers of meat freshness. The multi-level competitive coordination process between the metal-polyphenol system and amine substances endows the system with color changes far beyond those of traditional dyes (reaching a detection limit of 300 ppb). By integrating convolutional neural network (CNN) technology, an online platform is developed for monitoring meat freshness, achieving an overall detection accuracy rate of 99.83%. This environmentally friendly, economically viable MPN-CSA that monitors the freshness of meat in complex storage environments can be incorporated into food packaging boxes, enabling consumers and suppliers to assess the freshness of meat in real-time, thus helping to reduce food waste and prevent foodborne illnesses.
研究领域:
食品科学
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化学传感
·
人工智能应用
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产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2026
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IF 13.8
Yanqiu Zhang,Mingchuan Yang,Lejia Shi,Biaohua Zhang,Ruixia Dong,Yao Zhang,Wenxi Li,Xiangchun Zhang,Dongxu Wang
EGCG nanoparticles (ENPs) enhance the antibacterial activity by disrupting biofilms and membrane structures. • Zein/ENP composite films show improved mechanical properties and water barrier performance. • The active Zein/ENP films successfully double the shelf life of strawberries and apples. • Biodegradable Zein/ENP films exhibit ideal environmental and biological safety. Tea polyphenols (−)-epigallocatechin-3-gallate (EGCG) oxidation self-assembled nanocomposites (ENP) were synthesized through a one-step self-assembly process without templating or seeding agents and incorporated into zein-based edible films for the purpose of fruit preservation. The ENP nanocomposites maintained the core structural characteristics of EGCG and demonstrated excellent antioxidant activity, and antibacterial properties against E. coli and S. aureus . As a coating, ENP significantly reduced strawberry decay, weight loss, and nutrient degradation during storage. When integrated into zein films, ENP improved structural density, mechanical properties, water vapor permeability, antioxidant capacity, and light transmittance, without affecting the crystallinity of the zein-based films. The zein-based ENP edible films effectively extended the shelf life of strawberries and fresh-cut apples, surpassing the performance of both pure zein and polyethylene films. Mechanistic investigations have demonstrated that ENP compromised bacterial biofilm and structure integrity, leads to the leakage of intracellular contents, and inhibited ATPase activity. Biological safety assessment showed that the ENP nanocomposites exhibited negligible toxicity to zebrafish and seed germination, and the zein-based ENP film has ideal degradation performance. Taken together, zein-based films incorporating ENP offer a sustainable, effective, and safe approach for packaging in fruit preservation. To provide a simple and eco-friendly packaging solution, this study integrated tea polyphenol oxidative self-assembled composites (ENP) into zein-based edible films. This integration enhanced the mechanical strength, moisture resistance, and light transmittance of the films without disrupting the crystallinity of the natural zein. The active zein/ENP films exhibited robust antioxidant and antimicrobial activities, significantly extending the shelf life of strawberries and fresh-cut apples. Download: Download high-res image (360KB) Download: Download full-size image
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DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
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2020
·
IF 7.183
Yanchao Xu,Yirong Xiao,Wentian Zhang,Hongjun Lin,Liguo Shen,Renjie Li,Yang Jiao,Bao-Qiang Liao
Synthesis of a continuous and intergrown metal-organic framework (MOF) membrane remains a challenging issue due to the grain boundary defects and intercrystalline cracks in MOF thin film . In this study, a ZIF-8 membrane was fabricated by using a plant polyphenol tannic acid (TA) layer as an intermediate layer to overcome this issue. Specifically, a TA layer was constructed on a polyethersulfone substrate via a facile and environmentally friendly aqueous coating process . Thereafter, the above modified membrane was immersed into a Zn(Ac)2 and Hmim mixture solution for in-situ growing a ZIF-8 thin film on membrane surface. It was found that the abundant pyrogallol groups in TA layer could coordinate with Zn 2+ ions, promote heterogeneous nucleation and contribute to a continuous and intergrown ZIF-8 thin film. The as-prepared ZIF-8 membrane showed a pure water permeance of 3.6 L m −2 h −1 bar −1 with NaCl and Na 2 SO 4 rejections of 64.7% and 92.2% respectively, overwhelming the controlled ZIF-8 membrane without TA as intermediate layer in both permeability and selectivity. This membrane is one of few MOF membranes that have a divalent salt rejection higher than 90%, demonstrating its great potential application in efficient desalination.
研究领域:
材料科学
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环境工程
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DOI
高影响因子
Acta Pharmaceutica Sinica B
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2022
·
IF 14.903
Zhejie Chen,Wei Hao,Caifang Gao,Yangyang Zhou,Chen Zhang,Jinming Zhang,Ruibing Wang,Yitao Wang,Shengpeng Wang
With the development of synthesis technology, modified messenger RNA (mRNA) has emerged as a novel category of therapeutic agents for a broad of diseases. However, effective intracellular delivery of mRNA remains challenging, especially for its sensitivity to enzymatic degradation. Here, we propose a polyphenol-assisted handy delivery strategy for efficient in vivo delivery of IL-10 mRNA. IL-10 mRNA binds to polyphenol ellagic acid through supramolecular binding to yield a negatively charged core, followed by complexing with linear polyetherimide and coating with bilirubin-modified hyaluronic acid to obtain a layer-by-layer nanostructure. The nanostructure specifically up-regulated the level of IL-10, effectively inhibited the expression of inflammatory factors, promoted mucosal repair, protected colonic epithelial cells against apoptosis, and exerted potent therapeutic efficacy in dextran sulfate sodium salt-induced acute and chronic murine models of colitis. The designed delivery system without systemic toxicity has the potential to facilitate the development of a promising platform for mRNA delivery in ulcerative colitis treatment.
研究领域:
药学
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分子生物学
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纳米技术
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炎症性肠病
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2022
·
IF 5.999
Xu Ding,Fengxia Wang,Hui Hu,Sarah Imhanria,Wei Wang,Ji Zhang
Green fabrication of unique structural nanoparticles has always been of increasing interest in many fields. Herein, a facile and green strategy of fabricating catkin-like CuAg nanocomposites using tea-polyphenols as reduction agent is reported. As-prepared nanocomposites have been characterized by a series of analysis. Physical characterizations show the synthesised of nanocomposites whose catkin-like special morphology. The electrochemical detection hydrogen peroxide (H 2 O 2 ) results show that, catkin-like CuAg nanocomposites have good sensitivity, stability and anti-interference and it could detect without any additional mediator or enzyme . Specifically, it shows good H 2 O 2 sensitivity of 2.55 μA mM −1 cm −2 with range of 0.1–120 mM. Therefore, the catkin-like CuAg nanocomposites prepared by an environmental-friendly synthetic strategy, would provide a good reference for other green syntheses in the future.
研究领域:
材料科学
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环境科学
·
分析化学
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DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
·
2023
·
IF 10.9
Di Chuan,Huan Hou,Yuelong Wang,Min Mu,Jinglun Li,Yangmei Ren,Na Zhao,Bo Han,Haifeng Chen,Gang Guo
Due to the hypoxic state of the tumor microenvironment (TME), photodynamic therapy (PDT) suffers from insufficient ROS production. The metal-polyphenol network-mediated Fenton reaction can generate reactive oxygen species (ROS) by consuming H 2 O 2 in TME, improving the inadequate ROS generation problem of PDT. Therefore, synergistic therapy combining PDT and Fenton response-based CDT is a promising approach for cancer treatment. Herein, a metal-polyphenol nanocomposite was deposited with gallic acid grafted hyaluronic acid and Fe 3+ to contrast a Ce6 nano-delivery system ( [email protected] NPs) for melanoma synergistic therapy. [email protected] NPs could be used as a Fenton reagent to induce the ·OH production and enhance the PDT effect of Ce6 to a certain extent. After 4 h of cellular uptake, the fluorescence intensity of Ce6 in the [email protected] NPs group was higher than 3 times that in the Ce6 group. The intracellular ROS generation level of the [email protected] NPs (L) group combining CDT and PDT was higher than that of the Ce6 group and [email protected] NPs group. In vitro and in vivo anti-melanoma studies show that [email protected] NPs (L) group exhibited better anti-melanoma than other groups. Together, [email protected] NPs provide a promising synergistic treatment potential for melanoma.
研究领域:
癌症治疗
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纳米技术
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生物医学工程
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药学
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DOI
高影响因子
JOURNAL OF CONTROLLED RELEASE
·
2023
·
IF 10.8
Fang Zhu,Jianhua Zhang,Jian Zhong,Tianyou Wang,Yiwen Li,Zhipeng Gu
Iron-overload diseases are characterized by a variety of symptoms resulting from excessive iron stores, oxidative stress and consequent end-organ damage. Deferoxamine (DFO) is an iron-chelator that can protect tissues from iron-induced damage. However, its application is limited due to its low stability and weak free radical scavenging ability. Herein, natural polyphenols have been employed to enhance the protective efficacy of DFO through the construction of supramolecular dynamic amphiphiles , which self-assemble into spherical nanoparticles with excellent scavenging capacity against both iron (III) and reactive oxygen species (ROS). This class of natural polyphenols-assisted nanoparticles was found to exhibit enhanced protective efficacy both in vitro in an iron-overload cell model and in vivo in an intracerebral hemorrhage model. This strategy of constructing natural polyphenols- assisted nanoparticles could benefit the treatment of iron-overload related diseases with excessive accumulation of toxic or harmful substances.
研究领域:
医学
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药学
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DOI
PubMed
高影响因子
LANGMUIR
·
2024
·
IF 3.7
Mengyue Wang,Yu Wang,Nanjiong Pang,Mingyao Wang,Yunxiang He,Xiaoling Wang,Junling Guo
Ubiquitous antibiotics threaten human health and ecosystem sustainability, and existing removal strategies, especially conventional multistep water treatments, are primarily limited by the antibiotic-specific removal capability. Here, we explore the natural biomass, plant polyphenols, in the capture of various antibiotics with a facile treatment─polyphenol-mediated antibiotic-independent supramolecular coagulation (PMAC). The PMAC shows a superior performance in removing five tetracyclines and quinolones (up to 98.54%), even under complex environmental parameters, including different pH, the presence of inorganic particles and ionic strength, and the presence of conventional colloid-associated contaminants. Our mechanistic studies suggested that PMAC is capable of exerting multiple molecular interactions with various antibiotics, and the coordination-driven self-assembly further destabilizes the phenolic–antibiotic nanocomplexes, enabling an antibiotic-independent coagulation. Collectively, the combination of efficient remediation with inexpensive biomass suggests a simple and scalable method for the sustainable removal of antibiotics. Our strategy shows great promise as a cost-effective, facile approach to eliminate antibiotics capable of being integrated into the currently existing water treatment systems.
研究领域:
环境科学
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生物化学
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DOI
PubMed
高影响因子
BIOCHEMICAL JOURNAL
·
2023
·Vol. 480
·期 21
·1753-1766
·
IF 4.1
Cui Mengyu,Wei Yifeng,Tan Jason,Li Tong,Jiao Xinan,Zhou Yan
Phloroglucinol (1,3,5-trihydroxybenzene) is an important intermediate in the degradation of flavonoids and tannins by anaerobic bacteria. Recent studies have shed light on the enzymatic mechanism of phloroglucinol degradation in butyrate-forming anaerobic bacteria, including environmental and intestinal bacteria such as Clostridium and Flavonifractor sp. Phloroglucinol degradation gene clusters have also been identified in other metabolically diverse bacteria, although the polyphenol metabolism of these microorganisms remain largely unexplored. Here, we describe biochemical studies of polyphenol degradation enzymes found in the purple non-sulfur bacterium Rubrivivax gelatinosus IL144, an anaerobic photoheterotroph reported to utilize diverse organic compounds as carbon sources for growth. In addition to the phloroglucinol reductase and dihydrophloroglucinol cyclohydrolase that catalyze phloroglucinol degradation, we characterize a Mn2+-dependent phloretin hydrolase that catalyzes the cleavage of phloretin into phloroglucinol and phloretic acid. We also report a Mn2+-dependent decarboxylase (DeC) that catalyzes the reversible decarboxylation of 2,4,6-trihydroxybenzoate to form phloroglucinol. A bioinformatics search led to the identification of DeC homologs in diverse soil and gut bacteria, and biochemical studies of a DeC homolog from the human gut bacterium Flavonifractor plautii demonstrated that it is also a 2,4,6-trihydroxybenzoate decarboxylase. Our study expands the range of enzymatic mechanisms for phloroglucinol formation, and provides further biochemical insight into polyphenol metabolism in the anaerobic biosphere.
研究领域:
微生物学
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生物化学
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环境科学
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DOI
PubMed
高影响因子
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