INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Chaoyi Shen,Zian Deng,Jingshan Rao,Zhichao Yang,Yonghui Li,Di Wu,Kunsong Chen
In this work, multi-fluid mixing solution blow spinning was applied to develop gelatin/pullulan composite nanofibers, and then the nanofibers were glycated to enhance the physical properties. The results show that the grafting degree of the nanofibers increased significantly from 17.5 % to 36.0 % as the glycation time increased, and the morphology results indicated that 72 h of glycation did not destroy the structure of the nanofibers. FTIR results show that the glycation consumed the the–NH 2 groups, cleaved sugar units of polysaccharide, and affected the secondary structure of the protein. The glycation enhanced the thermal stability and improved the rigidity of the nanofibers. Besides, after 120 h of glycation, the water contact angle of nanofibers increased from 0° to 79.1°, and the water vapor transmission rates decreased from 12.49 to 8.97 g mm/m 2 h kPa, indicating the enhanced hydrophobicity and barrier properties. In addition, the glycation improved the water stability of the nanofibers, which increased the applicability of the gelatin/pullulan nanofibers in food packaging. The present work provides a green and efficient method for improving the physical properties of gelatin/pullulan nanofibers.
研究领域:
材料科学
·
纳米技术
·
生物技术
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Tian Wang,Boya Hao,Shilin Xu,Jie Meng,Tao Wen,Jian Liu,Haiyan Xu
RNA interference is a very useful tool for clinical treatment as well as mechanistic studies of leukemia. However, leukemia cells are known as hard-to-transfected by non-virus carriers. The low capacity of endocytosis and lysosomal escape of synthetic carriers are two obstacles for successful RNAi in leukemia cells. In this work, we established a universal powerful strategy for mediating effective RNAi in different types of leukemia cells by sequentially using spermine-modified pullulan (PS) as siRNA carrier and desloratadine (DL) to promote the lysosomal escape. It was shown that the complex of PS and siRNA could be largely internalized by human T-cell acute lymphoblastic leukemia cells, acute myeloid leukemia cells and chronic myeloid leukemia cells in serum-containing media, and the internalized complex was able to escape from the lysosomes by the aid of DL, resulting in effective RNAi against the key genes for the different leukemia cells.
研究领域:
肿瘤学
·
分子生物学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Siyao Cheng,Cheng Zhang,Junjian Li,Xihao Pan,Xinrang Zhai,Yingzhi Jiao,Yan Li,Wei Dong,Xiaoliang Qi
Magnetic nanoparticles that can be employed as Fenton-like catalysts Fenton-like catalysts are attractive materials for degrading antibiotics. In this study, we facilely prepared novel magnetic pullulan ( MP ) hydrogels by doping modified magnetic nanoparticles into pullulan matrices, which could enhance catalytic degradation performance and strengthen the stability of resulting hydrogels. This is the first time that MP hydrogels have been fabricated successfully and used as Fenton-like catalysts for tetracycline hydrochloride (TCH) degradation. MP hydrogels were characterized and their catalytic TCH degradation abilities were also investigated. The optimized conditions (pH value, Fe 3 O 4 content, H 2 O 2 content and TCH concentration) for TCH degradation were investigated. The optimized system showed excellent degradation efficiency for TCH. Further, the degradation mechanism was comprehensively studied. Finally, synthesized MP hydrogels showed impressive reusability and stability in the cycle experiment. Thus, our findings would open new possibilities to develop magnetic hydrogels in eliminating antibiotic contaminants.
研究领域:
环境科学
·
材料科学
·
化学工程
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Mingke Ding,Weijie Wu,Ruiling Liu,Ben Niu,Huizhi Chen,Xiangjun Fang,Hangjun Chen,Chaoyi Shen,Haiyan Gao
Blueberries are susceptible to microbial contamination and mechanical damage after harvesting, thereby accelerating their quality deterioration. Therefore, in the present work, thyme essential oil@halloysite nanotubes (TEO@HNTs)-loaded pullulan/gelatin/PVA (PGP) aerogels with antimicrobial activities and cushioning properties were developed to address these issues. The results showed that TEO achieved a 91.1 % encapsulation efficiency in HNTs and hydrogen bonding interactions were formed between TEO and HNTs. TEO@HNTs improved the crystallinity, thermal stability, compression properties, and surface hydrophobicity of the PGP aerogels. The TEO@HNTs-loaded aerogels exhibited a sustained release of TEO and antimicrobial activity against E. coli (inhibition zone of 13.92 mm), S. aureus (inhibition zone of 16.55 mm), and B. cinerea . Moreover, the aerogels offered good cushioning for blueberries when subjected to mechanical impact, thus maintaining their quality during storage. In addition, cell cytotoxicity analysis showed that cell viability exceeded 94 %, indicating the excellent biocompatibility of the TEO@HNTs-loaded aerogels. The above results suggested promising prospects for the development of a multifunctional aerogel to maintain the quality of food products, such as blueberries, which are susceptible to microbial contamination and mechanical damage.
研究领域:
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Polymer Materials
·
2025
·
IF 4.7
Zuodong Qin,Jin Yan,Leyong Wang,Meifeng Wang,Asad Nawaz,Zhenmin Cao,Gregory J Duns,Wubliker Dessie,Yao Chen,Xiaofang Luo
Burns are one of the most common causes of skin barrier damage and are prone to a range of problems, including wound infection. The development of a multifunctional dressing that is self-adhesive, anti-infective, and also provides sustained drug release is essential. In this study, polydopamine microspheres encapsulating an antimicrobial peptide were introduced into pullulan and gelatin hydrogels, and microsphere hydrogel composites were prepared in order to enhance the certain self-adhesive and antimicrobial properties. Antibacterial experiments showed that the composite hydrogel inhibited Staphylococcus aureus and Escherichia coli by 100%. The composite also showed good cytocompatibility, with cell survival rates above 90% in the AMP@PDA/Pu/Gel group when the microsphere concentration was 5 mg/mL. Mouse burn experiments showed that AMP@PDA/Pu/Gel hydrogels could also provide good bacterial inhibition. In addition, hydrogels reduced inflammation and promoted the formation of collagen and blood vessels. Furthermore, we selected the corresponding inflammatory factors (TNF-α, TGF-β1, TGF-β3) to test the anti-inflammatory properties of the composite hydrogel in burn wounds. The findings reveal that AMP@PDA/Pu/Gel hydrogels have good prospects for application in anti-infection treatment, wound repair and a type of burn wound dressing.
研究领域:
生物材料
·
组织工程
·
药学
·
生物医学工程
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
Current Drug Delivery
·
2023
·Vol. 20
·期 10
·1465-1473
·
IF 2.4
Huang Ren F.,Guo Yan,Yao Chaoling,Wu Wanming,Ou Linyang
Background: Many dyes or radioactive markers used for sentinel lymph node (SLN) have the shortcomings of false positive and radiation injury. Indocyanine green (ICG) seems to have a lower false positive rate and tissue damage, without a clear field of vision during the operation.
Methods: For the shortcomings, we successfully synthesized three anionic pullulan materials, changed the degree of hydrophobic for size controlling (< 50nm) to prepare CHP nanoparticles (NPs) and changed the succinyl degree to prepare CHPC NPs with different negative surface potential.
Results: The size of those NPs were less than 50nm under (transmission electron microscope) TEM, with hydrodynamic size of 90.67 ± 2.2 nm of CHP, 105.8 ± 1.7 nm of CHPC1 and 115.9 ± 2.3 nm of CHPC2. Moreover, the Zeta potential of CHP, CHPC1 and CHPC2 were -1.9 ±
0.2 mV, -9.6 ± 0.3 mV and -19.4 ± 0.7 mV. The size of ICG-loading CHP, CHPC1 and CHPC2 NPs increased to 109.4 ± 2.7 nm, 113.8 ± 1.2 nm and 30.6 ± 3.5 nm, as the zeta potential decreased to -2.7 ± 0.4 mV, -12.5 ± 1.6 mV and -23.1 ± 1.2
mV. With the increasing degree of succinyl, the size increased and the zeta potential decreased. At the same time, the higher degree of succinyl drug-loading NPs have lower release and have increased the stability of ICG. We found that the blank-NPs had no significant toxicity to normal cells
(HSF), as the ICG@CHP group had larger toxicity than the CHPCs and control. Moreover, the cellular uptake was decreased with the increased degree of succinyl. Conclusion: In this study, we successfully prepared CHPC2 carriers with the maximum negative surface charge, for follow-up research
and providing new ideas for SLN.
研究领域:
药学
·
纳米技术
·
生物医学工程
·
肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2026
·
IF 12.5
Kaipei Luo,Qiuxia Li,Siyin Chen,Qingya Jia,Yuxin Wu,Weiwei Liao,Yufei Su,Liping Chen,Xiao Wang,Zhimin Chen,Xiaofang Li,Ling Li,Lu Yang
Astragaloside IV (ASIV) is a triterpenoid saponin with promising potential in treating ulcerative colitis (UC), but its efficacy is hindered by poor solubility and rapid systemic clearance. Gliadin, an amphiphilic protein, has been widely used as a nanocarrier. Here, the novel edible pullulan-stabilized gliadin nanocomplexes (PL-Gli NPs) were developed to deliver ASIV. Interaction force analysis revealed that the formation of PL-Gli NPs involved in electrostatic interaction, hydrophobic interaction, and hydrogen bonding. ASIV was encapsulated into PL-Gli NPs via hydrophobic interaction to form ASIV-loaded nanocomplexes (ASIV@PL-Gli NPs). The resulting nanocomplexes had a particle size of (381.5 ± 5.3) nm and high encapsulation efficiency of 93.00 %. Notably, the stability of ASIV@PL-Gli NPs was improved under harsh conditions owing to the incorporation of pullulan. Furthermore, ASIV@PL-Gli NPs exhibited sustained release, with only 63.69 % cumulative release of ASIV at 10 h. Importantly, ASIV@PL-Gli NPs markedly enhanced anti-UC efficacy of ASIV, evidenced by a 51.81 % increase in colon length, a 53.16 % reduction in disease activity index scores, and significant decreases in pro-inflammatory cytokine levels. This work first employs pullulan and gliadin as oral nanocarriers for the delivery of ASIV, providing great potential for functional foods in UC treatment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2020
·
IF 4.006
Yifu Chu,ChengCheng Gao,Xiaoya Liu,Ni Zhang,Tian Xu,Xiao Feng,Yuling Yang,Xinchun Shen,Xiaozhi Tang
This study was aimed at evaluating the effect of pullulan coating incorporated with cinnamon essential oil (CEO) nanoemulsion on the shelf life and senescence of fresh strawberries during room storage. Pullulan-CEO nanoemulsion prepared using ultrasound treatment had smaller particle size (162.1 nm) and more uniform distribution of oil droplets. After six days of storage, pullulan-CEO nanoemulsion coating remarkably lowered the loss in fruit mass, firmness, total soluble solids and titratable acidity of strawberries. In addition, pullulan-based nanoemulsion coated strawberries showed the strongest antimicrobial activity against bacteria and molds (2.544 and 1.958 log CFU/g, respectively) compared to control and pure pullulan coating groups. It is mainly due to the antimicrobial activities of CEO dispersed in nano size in pullulan matrix. This study revealed that pullulan-CEO nanoemulsion coating showed great potential to prolong the shelf life of fresh strawberries during room storage.
研究领域:
食品科学
·
植物病理学
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2014
·Vol. 6
·期 23
·9198-9201
·
IF 1.938
Rui Wang,Jian Wu,Fang Zhang,Liu Wang,Feng Ji
1% (w/v) concentration of pullulan as a cross-priming isothermal amplification (CPA) reaction additive has a great acceleration effect on nucleic acid amplification. Using this property of pullulan, on-point specific detection of nucleic acid by lateral-flow dipstick (LFD) within 20 minutes was achieved with initial DNA template of 750 copies.
研究领域:
分子生物学
·
食品科学
·
植物遗传学
✓ 已验证
产品关联
DOI
高影响因子
Catalysts
·
2018
·Vol. 8
·期 1
·13
·
IF 3.465
Li Xu,Guli Cui,Caixia Ke,Yanli Fan,Yunjun Yan
A kind of pH-responsive particle was synthesized using modified pullulan polysaccharide. The synthesized particle possessed a series of merits, such as good dispersity, chemical stability and variability of particle size, making it a suitable carrier for enzyme immobilization. Then,Burkholderia cepacialipase (BCL), a promising biocatalyst in transesterification reaction, was immobilized on the synthesized particle. The highest catalytic activity and immobilization efficiency were achieved at pH 6.5 because the particle size was obviously enlarged and correspondingly the adsorption surface for BCL was significantly increased. The immobilization enzyme loading was further optimized, and the derivative lipase was applied in chiral resolution. Under the optimal reaction conditions, the immobilized BCL showed a very good performance and significantly shortened the reaction equilibrium time from 30 h of the free lipase to 2 h with a conversion rate of 50.0% andeesat 99.2%. The immobilized lipase also exhibited good operational stability; after being used for 10 cycles, it still retained over 80% of its original activity. Moreover, it could keep more than 80% activity after storage for 20 days at room temperature in a dry environment. In addition, to learn the potential mechanism, the morphology of the particles and the immobilized lipase were both characterized with a scanning electron microscope and confocal laser scanning microscopy. It was found that the enlarged spherical surface of the particle in low pH values probably led to high immobilized efficiency, resulting in the improvement of enantioselectivity activity in chiral resolution.Keywords:immobilization;Burkholderia cepacialipase;chiral resolution;1-phenylethanol;pullulan
研究领域:
生物化学
·
酶工程
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2022
·
IF 5.518
Nitong Bu,Liying Huang,Guoyu Cao,Huanglong Lin,Jie Pang,Lin Wang,Ruojun Mu
Biopolymers , such as polysaccharides are increasingly concern in preparation of food packaging. However, those materials have weakness in properties of mechanics, hydrophobicity , and antibacterial, which limit their applications. In this work, we prepared emulsion (K-PL-CEOs) films with double network (DN) matrix by using konjac glucomannan (KGM) and Pullulan (PL), and incorporated with cellulose nanofibrils (CNF)-stabilized tea tree essential oil (TTEO) Pickering emulsions (CEOs). The effect of CEOs content on the structural and physicochemical properties of K-PL films was investigated. The results showed that CEOs were well dispersed in K-PL film forming matrix. The addition of CEOs could improve the thermal stability, UV-shielding, water resistance and mechanical properties of K-PL-CEOs films. Moreover, K-PL-CEOs films exhibited good antibacterial activity against E. coli and S. aureus . This paper demonstrated that K-PL-CEOs films can be potentially applied as food packaging.
研究领域:
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
MATERIALS TECHNOLOGY
·
2022
·
IF 3.297
Shichao Wu,Xiaobing Yang,Xiangrui Yang
Due to the complexity of cancer, combination therapy has been widely used in the clinic. The use of two kinds of anticancer agents with a synergistic effect may achieve excellent therapeutic effects, especially against drug-resistant tumours. In this paper, MTX and HCPT loaded pullulan nanoparticles (MHNPs) were prepared for tumour-targeted synergistic therapy. In the nanoparticles, MTX could serve as both a targeting ligand and a cytotoxic agent. The MHNPs possessed a size of 185.7 ± 16.7 nm and a sustained release profile of the dual drug over a period of 48 h. The cytotoxicity assays stated that the MHNPs exhibited an enhanced killing ability to HeLa cells than the individual drug loaded NPs. These results suggest that the MHNPs could be a potential carrier with an efficient antitumor effect for tumour-targeted drug delivery.
研究领域:
药学
·
癌症研究
·
纳米技术
·
药物递送系统
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）