MOLECULAR NUTRITION & FOOD RESEARCH
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2017
·Vol. 61
·期 12
·1700332
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IF 4.323
Xiaoyao Ma,Yuan Zhang,Zengyong Wang,Yunbing Shen,Man Zhang,Quandeng Nie,Yuanyuan Hou,Gang Bai
Scope Ursolic acid (UA) is a pentacyclicterpenoid carboxylic acid that is present in a wide variety of plant foods. There are many beneficial health effects that are attributed to the properties of UA. However, the specific cellular targets of UA and the mechanism underlying downstream signal transduction processes linked to the anti-inflammation pathway have not been thoroughly elucidated to date. Methods and results Chemical biology strategies such as target fishing, click reaction synthesis of a UA probe and molecular imaging were used to identify potential target proteins of UA. Cysteinyl aspartate specific proteinase 3 (CASP3) and its downstream signaling pathway were verified as potential targets by molecular docking, intracellular enzyme activity evaluation and accurate pathway analysis. The results indicated that UA acted on CASP3, ERK1 and JNK2 targets, alleviated inflammation-associated downstream multiple signal transduction factors, including ERK1, NF-κB and STAT3, and exhibited anti-inflammation activities. Conclusion As a natural dietary supplement, UA demonstrated anti-inflammation activity via inhibition of CASP3 and shows the potential to improve the therapy effect of several inflammation-associated diseases.
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Materials Science & Engineering C-Materials for Biological Applications
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2017
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IF 4.164
Wenjun Zhao,Jiayi Jin,Huimin Wu,Shengfu Wang,Chuanqi Fneg,Shuijin Yang,Yu Ding
The Cu@Pt/C nanocomposites have been synthesized via two-step reduction method. Electrochemical observations showed that the Cu@Pt/C had better electrocatalytic activity for the reduction of hydrogen peroxide than Pt/C, with a wide linear range between 0.50 μM and 32.56 mM, a high sensitivity of 351.3 μA mM − 1 cm − 2 , and a low detection limit of 0.15 μM (signal/noise = 3). Furthermore, the sensor based on Cu@Pt/C has potential applications due to its excellent long-time stability, good reproducibility and acceptable selectivity.
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JOURNAL OF ALLOYS AND COMPOUNDS
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2016
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IF 3.014
Jiayi Jin,He Mei,Huimin Wu,Shengfu Wang,Qinghua Xia,Yu Ding
The Cu 2 O@Pt nanoparticles were prepared by a two-step method, using Vulcan XC-72 carbon as support (donated as Cu 2 O@Pt/C). Transmission electron microscopy , X-ray diffraction and X-ray photoelectron spectroscopy were used to characterize the resulting Cu 2 O@Pt core-shell nanoparticles. The chemically modified electrode based on Cu 2 O@Pt/C nanocomposites was employed to eliminate the interference from ascorbic acid and uric acid for the sensitive and selective determination of dopamine. The electrochemical results indicate that the Cu 2 O@Pt/C nanocomposites show better electrocatalytic activity for the oxidation of dopamine than Pt/C nanocomposites with a linear range from 10 nM to 1027.16 μM, a detection limit of 3 nM (S/N = 3) and sensitivity of 638.0 μA mM −1 cm −2 . Moreover, the sensor exhibits excellent long-time stability, good reproducibility and can be applied for practical application.
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SENSORS AND ACTUATORS B-CHEMICAL
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2016
·
IF 4.758
Bin Wang,Yuanya Wu,Yanfen Chen,Bo Weng,Changming Li
A flexible free-standing paper sensor was fabricated for the first time via in situ growth of Cu nanoflower on reduced graphene oxide (RGO) sheets towards amperometrically non-enzymatic detection of glucose. The low cost of copper (Cu) and graphene made the prepared sensor inexpensive in comparison with sensors made from noble metal catalysts . This Cu-RGO paper sensor exhibited high sensitivity and good selectivity when utilised in glucose sensing attributed to the synergistic effects of graphene paper and Cu nanoflowers. The combination of excellent catalytic activity , largely reactive surface area of Cu nanoflower and good conductivity of graphene paper enabled the sensor to perform well in glucose detection. The prepared glucose sensor holds a great promise for inexpensive flexible electrochemical non-enzymatic glucose sensors.
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CHINESE CHEMICAL LETTERS
·
2016
·
IF 1.947
Li-Qing Xie,Yan-Hui Zhang,Fei Gao,Quan-An Wu,Piao-Yang Xu,Shan-Shan Wang,Ning-Ning Gao,Qing-Xiang Wang
A nanocomposite of polyaniline/reduced graphene oxide (PANI-rGO) was synthesized using a hydrothermal method. The product was characterized by FT-IR, Raman spectra, XRD, SEM and TEM. Then the hybrid material of PANI-rGO and Nafion (PANI-rGO-NF) was prepared and used to modify glassy carbon electrode for the trace determination of dopamine (DA) employing differential pulse voltammetry (DPV). It was found that the hybrid material showed good catalytic activity toward the oxidation of DA, and no response to ascorbic acid (AA) and uric acid (UA) was observed, suggesting a high selectivity of the sensor toward DA. The peak currents were linearly correlated with the concentration of DA in the range from 0.05 μmol/L to 60.0 μmol/L (R = 0.996) and 60.0 μmol/L to 180.0 μmol/L (R = 0.996) with a detection limit of 0.024 μmol/L (S/N = 3). The modified electrode also exhibited excellent repeatability and stability.
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Journal of Physical Chemistry C
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2016
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IF 4.509
Yinghan Yan,Cuiling Zhang,Wei Gu,Caiping Ding,Xinchang Li,Yuezhong Xian
A facile and eco-friendly approach for the synthesis of water-soluble WS2 quantum dots (QDs) was developed via ultrasonication and a hydrothermal process from bulk WS2. In this strategy, the dispersity of bulk WS2 in aqueous phase was improved with the aid of a surfactant (hexadecyltrimethylammonium bromide, CTAB), which could shorten the exfoliation time and improve the exfoliation efficiency to form layered WS2 nanosheets. Through hydrothermal treatment, the nanosheets were further scissored into QDs with high quality. The QDs show excellent features with narrow size distribution, good water solubility, and stable fluorescence. We find that the fluorescence of WS2 QDs can be quenched by Fe3+ through photoinduced electron transfer, and a wide detection linear range for Fe3+ is acquired. It indicates that WS2 QD can be used as a “turn-off” probe for Fe3+. In the presence of lipoic acid (LA), the fluorescence was recovered due to the stronger interaction between LA and Fe3+ than WS2 QDs. A “turn-on” sensor for LA was developed with a linear range from 1 to 10 μM and a detection limit of 0.59 μM. The strategy might be suitable for the facile synthesis of other water-soluble transition metal dichalcogenide QDs. It is expected that the water-soluble QDs have great potential applications in biological system.
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SENSORS AND ACTUATORS B-CHEMICAL
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2016
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IF 4.758
Weibo Li,Dongping Qian,Qiuhong Wang,Yubin Li,Ning Bao,Haiying Gu,Chunmei Yu
In this work, an origami paper-based analytical device for glucose biosensor by employing fully-drawn pencil electrodes has been reported. The three-electrode system was prepared on paper directly by drawing with nothing more than pencils. By simple printing, two separated zones on paper were designed for the immobilization of the mediator and glucose oxidase (GOx), respectively. The used paper provides a favorable and biocompatible support for maintaining the bioactivities of GOx. With a sandwich-type scheme, the origami biosensor exhibited great analytical performance for glucose sensing including acceptable reproducibility and favorable selectivity against common interferents in physiological fluids. The limit of detection and linear range achieved with the approach was 0.05 mM and 1–12 mM, respectively. Its analytical performance was also demonstrated in the analysis of human blood samples. Such fully-drawn paper-based device is cheap, flexible, portable, disposable, and environmentally friendly, affording great convenience for practical use under resource-limited conditions. We therefore envision that this approach can be extended to generate other functional paper-based devices.
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MICROCHIMICA ACTA
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2016
·Vol. 183
·期 5
·1651-1657
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IF 4.831
Rao Hanbing,Ge Hongwei,Lu Zhiwei,Liu Wei,Chen Ziqi,Zhang Zhaoyi,Wang Xianxiang,Zou Ping,Wang Yanying,He Hua,Zeng Xianying
We report that copper nanoclusters (CuNCs) represent a viable fluorescent probe for ascorbic acid. The CuNCs were stabilized by tannic acid and characterized by high-resolution transmission electron microscopy, X-ray photoelectron spectroscopy, UV–vis, time-correlated single photon counting and fluorescence spectroscopy. It is found that the fluorescence of CuNCs (at excitation/emission peaks of 355/430 nm) is quenched by ferric ion due to electron-transfer between CuNCs and Fe(III) ions. If ascorbic acid is added, Fe(III) is reduced to Fe(II) and quenching is suppressed. The process is completed within 5 min. Under optimal conditions, a linear relation exists between fluorescence recovery and the concentration of ascorbic acid in the 0.5 μM to 10 μM concentration range. The detection limit is 0.11 μM. The method was applied to the determination of ascorbic acid in (spiked) fruit and vegetables and gave recoveries ranging from 89 to 110 %.
The fluorescence of tannic acid stabilized copper nanoclusters (TA-CuNCs) is quenched by Fe 3+ . Upon addition of ascorbic acid (AA), the redox reaction between Fe 3+ and ascorbic acid converts Fe 3+ to Fe 2+ , and this results in a dramatic fluorescence enhancement of the probe.
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Scientific Reports
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2016
·Vol. 6
·期 1
·1-8
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IF 5.228
Cheng Qin,Ji Liudi,Wu Kangbing,Zhang Weikang
Using starch as the carbon precursor and different-sized ZnO naoparticles as the hard template, a series of porous carbon materials for electrochemical sensing were prepared. Experiments of scanning electron microscopy, transmission electron microscopy and Nitrogen adsorption-desorption isotherms reveal that the particle size of ZnO has big impacts on the porous morphology and surface area of the resulting carbon materials. Through ultrasonic dispersion of porous carbon and subsequent solvent evaporation, different sensing interfaces were constructed on the surface of glassy carbon electrode (GCE). The electrochemical behaviors of ascorbic acid (AA), dopamine (DA) and uric acid (UA) were studied. On the surface of porous carbon materials, the accumulation efficiency and electron transfer ability of AA, DA and UA are improved, and consequently their oxidation signals enhance greatly. Moreover, the interface enhancement effects of porous carbon are also controlled by the particle size of hard template. The constructed porous carbon interface displays strong signal amplification ability and holds great promise in constructing a sensitive platform for the simultaneous determination of AA, DA and UA.
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BIOSENSORS & BIOELECTRONICS
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2016
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IF 7.476
Xiangyu Jiang,Fenghua Geng,Yongxiang Wang,Jinhua Liu,Peng Qu,Maotian Xu
Quantitative fluorescence turn-on and colorimetric detection of glutathione (GSH) with rapid speed, low cost have attained much attention. Herein, we developed a sensitive fluorescence turn-on and colorimetric sensor for GSH based on the inner-filter effect (IFE), which is the first time to select oxTMB and TMPyP as the IFE absorber and fluorophore pair, respectively. The absorption band of oxTMB matches well with the emission band of TMPyP in the IFE-based fluorescent assay. In the absence of GSH, the absorption peak of oxTMB at 652 nm significantly overlaps with the emission of TMPyP, resulting in the efficient IFE and inhibition of the fluorescence of TMPyP. In the presence of GSH, the absorption intensity at 652 nm decreases, generating the recovery of the fluorescence of TMPyP. Therefore, this approach is demonstrated to be a novel candidate for detection of GSH, with high sensitivity and selectivity. The linear dynamic range for the concentrations of GSH is between 0.1 μM to 20 μM along with a limit of detection (LOD) of about 30 nM (calculated LOD as 3 σ /slope). Finally, this novel sensor was successfully applied for GSH detection in fetal calf serum , and satisfactory recovery was achieved.
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JOURNAL OF SOLID STATE ELECTROCHEMISTRY
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2016
·Vol. 20
·期 5
·1377-1389
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IF 2.327
Hui Yuchen,Ma Xiaoyan,Qu Fengjin,Chen Fang,Yu Jie,Gao Yan
A facile strategy is developed to fabricate an Au-CMCD/PCMCD electrode with high efficiency, universal, and selectivity electrocatalysis through electro-depositing carboxymethyl-β-cyclodextrins with gold nanoparticles (Au-CMCD) followed by employing electro-polymerization carboxymethyl-β-cyclodextrin (PCMCD). The chemical structure, microstructure, electrochemical activity, and electrocatalytic properties of Au-CMCD/PCMCD electrode are investigated. Due to the synergistic effect of the PCMCD and Au-CMCD layers, the as-prepared electrode exhibits an outstanding enhancement of universal electrocatalytic activity to eight kinds of crucial biomolecules and drugs, such as chloramphenicol, thymine, and glucose. Then, as a target analyte, glucose is used to investigate the sensing performances of Au-CMCD/PCMCD electrode. The amperometric detection of glucose shows a wide linear range expanding to 0.001∼110 mM with a low detection limit of 0.99 μM. More importantly, the absorption effect of PCMCD is stronger to glucose than other interfering species, which is favorable to avoid the influence of possible interfering substances. Furthermore, the glucose sensors reveal good stability and repeatability.
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TALANTA
·
2016
·
IF 4.035
Jian Li,Ning Zhang,Qingqing Sun,Zhanming Bai,Jianbin Zheng
A novel imprinted silica matrix-poly(aniline boronic acid) hybrid for electrochemical detection of dopamine (DA) was developed. Boronic acid functionalized conducting polymer was electrochemically prepared on Au electrode. The number of covalent binding sites toward DA templates was controlled by potential cycles. A precursory sol solution of ammonium fluorosilicate (as cross-linking monomer) containing DA was spin-coated on the polymer modified electrode . Under NH 3 atmosphere, the hydroxyl ions were generated in the solution and catalyzed the hydrolysis of fluorosilicate to form silica matrix. After this aqueous sol-gel process, an inorganic framework around the DA template was formed and the imprinted hybrid for DA was also produced. As revealed by scanning electron microscopy, UV-vis spectroscopy and cyclic voltammetry characterization, DA was embedded in the imprinted hybrid successfully. The affinity and selectivity of the imprinted hybrid were also characterized by cyclic voltammetry . The imprinted hybrid showed higher affinity for DA than that for epinephrine, and little or no affinity for ascorbic acid and uric acid due to the combined effects of covalent interaction, cavities matching and electrostatic repulsion. The imprinted hybrid sensor exhibited a quick response (within 5 min) to DA in the concentration range from 0.05 to 500 μmol L −1 with a detection limit of 0.018 μmol L −1 . The prepared sensor was also applied to detect DA in real samples with a satisfactory result.
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