JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
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2024
·
IF 4.5
Xue Zhang,Jiang Chen,Yuan Yin,Shijun Xiao,Rui Zhang,Haiyang Guo,Tong Yang,Tongyu Zhou,Siyan Zhang,Yang Yang,Caili Bi,Xiao-Jun Li
Targeted delivery of chemotherapeutic agents to the cancerous cells is of fundamental importance and the key step of the targeted delivery system design is to choice a specific receptor. Glypican-3 (GPC3) is a known cell membrane-associated oncofetal proteoglycan that is specifically up-regulated in the fast-growing hepatocarcinoma cells but rarely expressed in the normal healthy liver. Therefore, GPC3 may be a perfect targeting receptor for delivery treatment of hepatocellular carcinoma (HCC). In the present study, a GPC3 specific-targeting nanoliposomal drug delivery system was constructed with GPC3 targeting peptide (named as L5 peptide) modification and anticancer drug cantharidin encapsulation, which aims to realize cantharidin targeted treatment of liver cancer. In our study, nanoliposome specifically targeting GPC3 receptor was successfully constructed with particle size of 127.9 nm and a spherical shape. The targeted liposomes were stable under 4 °C or room temperature for almost 15 days. The drug release from L5-modified liposomes was controlled and delayed. Their targeted delivery properties were characterized by relatively more accumulation in the GPC3 highly expressed HepG2 cells than that of GPC3 lowly expressed Huh-7 cells. The competition results also indicated that the liposomes with L5 peptide modification could competitively bind to GPC3 receptor. Moreover, the liposomes showed colocalization with the lysosome after 3 h incubation. The L5-modified liposomal CTD had an augmented cell growth inhibition and tumor inhibition than that of the free CTD on HepG2 cells and GPC3 highly expressed tumor mice. Our study establishes a new strategy for improving HCC treatment and chemotherapeutic agent targeting delivery.
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APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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2025
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IF 3.3
Guo Jing,Cui Jiayi,Xun Mingyue,Zhang Wencheng,Xian Mo,Zhang Rubing
L- threo -3-hydroxyaspartic acid (L-THA) is a non-proteinogenic amino acid that has garnered significant attention due to its diverse biological activities. However, the synthesis of L-THA through enzymatic and whole-cell catalysis requires the expensive substrate L-aspartic acid or L-asparagine, and co-substrate α-ketoglutarate, which limits their large-scale application. Here, this is the first report of engineering E. coli as a cell factory for de novo production of L-THA from glucose by fermentation. Firstly, the asnO gene encoding asparagine hydroxylases from Streptomyces coelicolor was heterologously expressed in E. coli to yield the L-THA producing strain. The formation and configuration of L-THA were characterized by LC–MS and HPLC after FDAA derivatization. Secondly, the pathway genes aspC and asnB , which encode aspartate aminotransferase and asparagine synthase, respectively, were overexpressed to enhance L-THA titer from 49.9 to 90.84 mg/L. Thirdly, the efforts were made to improve the key precursor L-aspartic acid pool by overexpressing the aspartase encoding gene aspA and knocking out aspartate kinase (AK) III encoding gene lysC . The best strain CC03 was obtained and L-THA titer reached 278.3 mg/L in a shake flask, representing an approximately 5.6-fold increase compared to the original strain. Ultimately, 2.87 g/L L-THA was obtained after 32 h fed-batch fermentation. This research underscores the potential use of E. coli fermentation as a feasible platform for de novo biosynthesis of L-THA from glucose, which is amenable to industrial application.
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BIOPROCESS AND BIOSYSTEMS ENGINEERING
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2017
·Vol. 40
·期 6
·901-910
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IF 1.87
Zhang Qiang,Gong Jin-Song,Dong Ting-Ting,Liu Ting-Ting,Li Heng,Dou Wen-Fang,Lu Zhen-Ming,Shi Jin-Song,Xu Zheng-Hong
3-Hydroxypropionic acid (3-HP) is an important platform chemical in organic synthesis. Traditionally, 3-HP was produced by chemical methods and fermentation process. In this work, a novel enzymatic method was developed for green synthesis of 3-HP. A yeast strain harboring nitrile-hydrolyzing enzyme was newly isolated from environmental samples using 3-hydroxypropionitrile (3-HPN) as the sole nitrogen source. It was identified to be Meyerozyma guilliermondii CGMCC12935 by sequencing of the 18S ribosomal DNA and internal transcribed spacer, together with analysis of the morphology characteristics. The catalytic properties of M. guilliermondii CGMCC12935 resting cells were determined, and the optimum activity was achieved at 55 °C and pH 7.5. The enzyme showed broad substrate specificity towards nitriles, especially 3-HPN, aminoacetonitrile and 3-cyanopyridine. The presence of Ag + , Pb 2+ and excess substrate inhibited the enzyme activity, whereas 5% (v/v) ethyl acetate had a positive effect on the enzyme activity. M. guilliermondii CGMCC12935 resting cells by addition of 3% glucose could thoroughly hydrolyze 500 mM 3-HPN into 3-HP within 100 h and the maximal accumulative production of 3-HP reached 216.33 mM, which was over twofolds than the control group with no additional glucose. And this work would lay the foundation for biological production of 3-HP in industry.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2024
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IF 5.3
Qinqin Zhang,Yipin Zhang,Chenyang Qi,Jie Chen,Haonan Hu,Guitao Tan,Jing Tu
Reactive oxygen species (ROS) play a vital role in wound healing process by fighting against invaded bacteria. However, excess ROS at the wound sites lead to oxidative stress that can trigger deleterious effects, causing cell death, tissue damage and chronic inflammation. Therefore, we fabricated a core–shell structured nanomedicine with antibacterial and antioxidant properties via a facile and green strategy. Specifically, Prussian blue (PB) nanozyme was fabricated and followed by coating a layer of epigallocatechin-3-gallate (EGCG)-derived polymer via polyphenolic condensation reaction and self-assembly process, resulting in PB@EGCG. The introduction of PB core endowed EGCG-based polyphenol nanoparticles with excellent NIR-triggered photothermal properties. Besides, owing to multiple enzyme-mimic activity of PB and potent antioxidant capacity of EGCG-derived polymer, PB@EGCG exhibited a remarkable ROS-scavenging ability, mitigated intracellular ROS level and protected cells from oxidative damage. Under NIR irradiation (808 nm, 1.5 W/cm 2 ), PB@EGCG (50 µg/mL) exerted synergistic EGCG-derived polymer-photothermal antibacterial activity against Gram-negative Escherichia coli ( E. coli ) and Gram-positive Staphylococcus aureus ( S. aureus ). In vivo therapeutic effect was evaluated using a S. aureus -infected rat model indicated PB@EGCG with a prominent bactericidal ability could modulate the inflammatory microenvironment and accelerate wound healing. Overall, this dual-functional nanomedicine provides a promising strategy for efficient antibacterial therapy.
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TOXICON
·
2024
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IF 2.6
Qian Zhang,Xiuzhi Fei,Yue Li,Hengwei Zhang,Lu Chen,Jianping Ruan,Ning Dong
ABSTRACT Fluoride is a double-edged sword. It was widely used for early caries prevention while excessive intake caused a toxicology effect, affected enamel development, and resulted in dental fluorosis. The study aimed to evaluate the protective effect and mechanism of Epigallocatechin-3-gallate (EGCG) on the apoptosis induced by fluoride in ameloblast-like cells. We observed that NaF triggered apoptotic alterations in cell morphology, excessive NaF arrested cell cycle at the G1, and induced apoptosis by up-regulating Bax and down-regulating Bcl-2. NaF activated the insulin-like growth factor receptor (IGFR), and phosphatidylinositol-3-hydroxylase (p-PI3K), while dose-dependently down-regulating the expression of Forkhead box O1 (FoxO1). EGCG supplements reversed the changes in LS8 morphology, the cell cycle, and apoptosis induced by fluoride. These results indicated that EGCG possesses a protective effect against fluoride toxicity. Furthermore, EGCG suppressed the activation of p-PI3K and the down-regulation of FoxO1 caused by fluoride. Collectively, our findings suggested that EGCG attenuated fluoride-induced apoptosis by inhibiting the PI3K/FoxO1 signaling pathway. EGCG may serve as a new alternative method for dental fluorosis prevention, control, and treatment.
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BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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2024
·
IF 2.5
Xinlei Liu,Rui Liu,Yancheng Wang
Drug-induced liver injury (DILI) occurs frequently and can be life-threatening. Increasing researches suggest that acetaminophen (APAP) overdose is a leading cause of drug-induced liver injury. Indole-3-carboxaldehyde (I3A) alleviates hepatic inflammation, fibrosis and atherosclerosis, suggesting a potential role in different disease development. However, the question of whether and how I3A protects against acetaminophen-induced liver injury remains unanswered. In this study, we demonstrated that I3A treatment effectively mitigates acetaminophen-induced liver injury. Serum alanine/aspartate aminotransferases (ALT/AST), liver malondialdehyde (MDA) activity, liver glutathione (GSH), and superoxide dismutase (SOD) levels confirmed the protective effect of I3A against APAP-induced liver injury. Liver histological examination provided further evidence of I3A-induced protection. Mechanistically, I3A reduced the expression of apoptosis-related factors and oxidative stress, alleviating disease symptoms. Finally, I3A treatment improved survival in mice receiving a lethal dose of APAP. In conclusion, our study demonstrates that I3A modulates hepatotoxicity and can be used as a potential therapeutic agent for DILI.
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International Journal of Electrochemical Science
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2024
·
IF 1.3
Mengling Xu,Junjie You,Chuanqing Du,Zhuotong Yu,Siqing Cheng
Prussian blue (PB) has been broadly recognized as promising cathode materials for sodium-ion batteries (SIBs) due to its large interstitial space and robust frame structure, whereas their applications are impeded by unsatisfied long-term cyclability and poor rate capability due to its inherent lattice defects. Herein, we report to fabricate the mesoporous hollow Fe 4 (Fe(CN) 6 ) 3 (one kind of PB) hierarchical nanocrystal architecture by the hydrothermal crystallization and HCl etching of the mesoporous precursor Fe 4 [Fe(CN) 6 ] 3 cubic particles, leading to high crystallinity and large specific surface area. The resultant Fe 4 [Fe(CN) 6 ] 3 cathode for SIBs exhibits a good reversibility and cycling stability with a capacity retention of 75.6 % at 0.5 C after 200 cycles as well as superb rate capability featuring a stable discharge specific capacity of 30.2 mAh g −1 at as high as 10 C, demonstrating the fast kinetics and low polarization of electrochemical reaction, which might be ascribed to the unique architecture and high crystallinity. This offers a new route to designing PB cathode of for SIBs with high rate capabilities.
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Laser & Photonics Reviews
·
2024
·
IF 9.8
Ruiqing Zhang,Huidong Xie,Fu Wang,Qiyu Zhao,Lingyi Meng,Zuobin Tang,Binbin Su,Hu Liu
The luminescence property of various compounds is developed in the photoelectric applications field, while the near-infrared (NIR) dual-band emission of compounds is extremely challenging. Herein, 0D Cu(I)-based organic–inorganic module (ETA) 3 Cu 6 I 9 is synthesized to achieve NIR and cyan dual-band emission in metal halide systems. Density functional theory (DFT) calculations combined with comprehensive spectroscopic data reveal its emission mechanism. Under the excitation at 371 nm, (ETA) 3 Cu 6 I 9 shows the broadband NIR emission peaking at 775 nm with a large wavelength, attributed to the triple-cluster-center ( 3 CC) transition. Meanwhile, under 286 nm excitation, (ETA) 3 Cu 6 I 9 shows a bright cyan emission peaking at 490 nm, attributed to the metal-to-ligand charge transfer (MLCT) or halide-to-ligand charge transfer (XLCT) transitions. Moreover, (ETA) 3 Cu 6 I 9 presents relatively superior thermal and air stability. Benefiting from the good stability of (ETA) 3 Cu 6 I 9 , the as-fabricated NIR-LED device demonstrates great potential in biological imaging and night vision. This study opens up a new way for designing new non-toxic NIR and cyan dual-band emission materials, which guides to synthesize new materials in metal halide field.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Qin-Chao Wang,Zhaoquan Peng,Sha He,Hao Chen,Jinglong Du,Huilin Zang,Xiaoge Li,Xiuqin Zhan,Jie Han
Sodium superionic conductor NaTi 2 (PO 4 ) 3 has attracted significant interest as an anode material for sodium-ion batteries (SIBs). However, its practical application is hindered by its low inherent electrical conductivity, particularly at low temperatures. In this study, oxygen vacancies (V O ) were introduced into NaTi 2 (PO 4 ) 3 nanoribbons to enhance sodium storage performance at low temperatures. X-ray diffraction with Rietveld refinement, electron paramagnetic resonance, and X-ray photoelectron spectroscopy confirm that NaTi 2 (PO 4 ) 3 -2 nanoribbons (NTP-2) exhibit the richest V O concentration. These V O , which bridge TiO 6 octahedra and PO 4 tetrahedra, significantly enhance the antibonding interactions of Ti1–O2 and P1–O1 bonds, while stabilizing the bonding in NaTi 2 (PO 4 ) 3 . The energy barrier for Na + migration is reduced to 0.40 eV involving the V O . The optimized NTP-2 anode demonstrates superior low-temperature performance, maintaining a capacity of 106.1 mAh g −1 (about 96.1 % of its initial capacity) at −20 °C after 300 cycles. Additionally, the NTP-2 anode exhibits a moderate Na + diffusion coefficient of 1.47 × 10 –11 cm 2 s −1 at −20 °C. Furthermore, the Na 3 V 2 (PO 4 ) 3 //NTP-2 full cell retains a capacity of 64 mAh g −1 at −20 °C after 250 cycles, highlighting its potential for low-temperature applications. By integrating oxygen vacancies and nanoengineering, both electronic and ionic conductivities are significantly enhanced in NaTi 2 (PO 4 ) 3 , positioning promising applications for SIBs in low-temperature environments.
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Journal of Zhejiang University-SCIENCE B
·
2018
·
IF 1.815
Yi-ying Zhao,Qiang Chu,Xu-er Shi,Xiao-dong Zheng,Xiao-ting Shen,Yan-zhen Zhang
To address the controversial issue of the toxicity of dental alloys and silver nanoparticles in medical applications, an in vivo-like LO2 3-D model was constructed within polyvinylidene fluoride hollow fiber materials to mimic the microenvironment of liver tissue. The use of microscopy methods and the measurement of liver-specific functions optimized the model for best cell performances and also proved the superiority of the 3-D LO2 model when compared with the traditional monolayer model. Toxicity tests were conducted using the newly constructed model, finding that four dental castings coated with silver nanoparticles were toxic to human hepatocytes after cell viability assays. In general, the toxicity of both the castings and the coated silver nanoparticles aggravated as time increased, yet the nanoparticles attenuated the general toxicity by preventing metal ion release, especially at high concentrations.
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Experimental and Therapeutic Medicine
·
2018
·Vol. 15
·期 5
·4284-4290
·
IF 1.41
Sha Feng,Jue Liu,Biao Cheng,Aiping Deng,Hong Zhang
It has been acknowledged that environmental stress is a risk factor for developing mental disorders. Chronic stress may contribute to the hyperactivation of the hypothalamic‑pituitary‑adrenal (HPA) axis and a sustained rise in the levels of glucocorticoids (GCs). A high concentration of corticosterone (CORT) damages neuronal PC12 cells. It has been reported that (‑)‑Epigallocatechin‑3‑gallate (EGCG), a major component of green tea, exhibits neuroprotective activity. However, the protective effect of EGCG on neuronal cells injured by CORT remains to be elucidated. The present study aimed to identify the effects of EGCG on CORT‑injured neuronal PC12 cells and its associated mechanisms of action. CORT‑injured PC12 cells were pretreated with EGCG with or without cyclopamine. Cell viability was assessed using an MTT assay, changes in cell morphology were observed using phase‑contrast microscopy, cellular apoptosis was assessed by Hoechst 33342 staining, cell proliferation was measured using a cell counting kit‑8 assay, mRNA levels were measured by reverse transcription‑quantitative polymerase chain reaction and protein expression was assessed using western blot analysis. The current study demonstrated that exposure to high concentrations of CORT induced cytotoxicity and downregulated the Sonic hedgehog pathway (Shh) in PC12 cells. These effects were attenuated by EGCG. However, the EGCG‑mediated neuroprotective effects, as well as upregulation of the Shh pathway were all attenuated by the Shh signaling inhibitor cyclopamine. These results indicate that EGCG protects PC12 cells from CORT‑induced neurotoxicity via activation of the Shh signaling pathway.
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MOLECULAR CELL
·
2024
·
IF 14.5
Wei Luo,Yunyuan Xu,Jie Cao,Xiaoyu Guo,Jingdan Han,Yuanyuan Zhang,Yuda Niu,Meiling Zhang,Yi Wang,Guohua Liang,Qian Qian,Song Ge,Kang Chong
While it is known that temperature sensors trigger calcium (Ca 2+ ) signaling to confer cold tolerance in cells, less is known about sensors that couple with other secondary messengers. Here, we identify a cold sensor complex of CHILLING-TOLERANCE DIVERGENCE 6 (COLD6) and osmotin-like 1 (OSM1), which triggers 2′,3′-cyclic adenosine monophosphate (2′,3′-cAMP) production to enhance cold tolerance in rice. COLD6, which is encoded by a major quantitative trait locus (QTL) gene, interacts with the rice G protein α subunit (RGA1) at the plasma membrane under normal conditions. Upon exposure to chilling, cold-induced OSM1 binds to COLD6, kicking out RGA1 from interaction. This triggers an elevation of 2′,3′-cAMP levels for enhancing chilling tolerance. Genetic data show that COLD6 negatively regulates cold tolerance and functionally depends on OSM1 in chilling stress. COLD6 alleles were selected during rice domestication. Knockout and natural variation of COLD6 in hybrid rice enhanced chilling tolerance, hinting design potential for breeding. This highlighted a module triggering 2′,3′-cAMP to improve chilling tolerance in crops.
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