Journal of Future Foods
·
2025
·
IF 7.2
Pengfei Dong,Shuang Wu,Youfa Wang,Ning Yu,Vijaya Raghavan,Jin Wang
Sesame allergy has emerged as a public health issue due to its persistent occurrence, severe symptomatic manifestations, and widespread incidence globally. Among sesame allergens, 2S albumins (Ses i 2) is one of the widely recognized allergens, and its precise detection is urgently needed to prevent sesame-related allergies. In this work, a composite structure was designed and synthesized by combining metal ion self-assembled metal organic frameworks (MOFs) and imine constructed covalent organic frameworks (COFs) (represented by MOF@COF), which constructing a high-sensitivity sensing system for identification and detection of Ses i 2. A novel hybrid material, comprising a strategically assembled combination of UIO-66-NH 2 and TA-COF, was crafted to yield a functionalized UIO-66-NH 2 @TA-COF composite, demonstrating remarkable electrochemical performance. As a result, a significant quantity of aptamer strands can adhere to the UIO-66-NH 2 @TA-COF surface resulting from robust π-π stacking interactions. With the change in the relative concentration of Ses i 2 from 5 to 650 nM, the UIO-66-NH 2 @TA-COF sensor exhibited a low detection limit of 0.13 nM, which demonstrates its extremely high sensitivity. In addition, the UIO-66-NH 2 @TA-COF sensor exhibited excellent reproducibility, stability and specific recognition ability to Ses i 2. Therefore, this innovative sensor exhibits great potential for real-world applications in food safety.
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MOLECULAR NEUROBIOLOGY
·
2025
·
IF 4.3
Li Ying,Wang Lei,Cao Yan,Lu Hujing,Zhou Jiawen,Zhao Panpan,Guan Xinying,Dong Zibo
Neuropathy is a common chronic complication of type 1 diabetes mellitus (T1D), an autoimmune disease in which neuroinflammation is considered a key pathological mechanism. P21-activated kinase 4 (PAK4) is a significant factor in this neuroinflammatory process. Higenamine hydrochloride (HGN) possesses anti-inflammatory properties. This study investigated the effects of HGN on T1D and its potential regulation of neuroinflammation via PAK4. We established a T1D mouse model using an intraperitoneal injection of streptozotocin (STZ), followed by HGN administration. The PAK4 inhibitor PF3758309 was given 2 weeks before the experiment ended. Pathological alterations in brain tissues were examined through Nissl and H&E staining. Cognitive function and behavior were assessed using the Morris water maze, elevated plus maze, and open field tests. Molecular docking, cellular thermal shift analysis (CETSA), drug affinity responsive target stability (DARTS) assays, immunofluorescence, and immunoblotting were employed to investigate the binding of HGN to PAK4, its nuclear translocation, and microglial polarization. HGN mitigated brain damage, memory loss, anxiety, and behavioral dysfunction. It also reduced the levels of proinflammatory cytokines (TNF-α and IL-1β) and markers (CD16b, CD11b, and iNOS). Mechanistically, HGN increased PAK4 stability and inhibited M1 polarization via the CRTC2-CREB pathway. PF3758309 diminished the protective effects of HGN and elevated the serum levels of brain damage indicators (S100β and NSE) and inflammatory factors. HGN effectively reduces neuroinflammation and neurological dysfunction in T1D by targeting PAK4, suggesting its potential as a therapeutic agent for diabetes-related complications. Graphical
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Peng-Wei Chen,Lin-Song Li,Yu-Meng Zhang,Bang-Bang Liu,Xianjiao Tang,Liping Chen,Xiaojing Yang,Mei-Xia Zhao
In recent years, most photosensitizers suffer from poor photothermal conversion efficiency (PCE), vulnerability to aggregated-caused quenching under physiological conditions, and are highly dependent on oxygen, which seriously limit their application in cancer therapy. In this study, DTIR780 was an aggregation induced emission (AIE) photosensitizer, which was synthesized through the originality of molecular engineering. Because DTIR780 had a special organelle targeting lysosomes and mitochondria, it had excellent PCE (PCE = 28.9 %), and could simultaneously generate Type I reactive oxygen species (ROS): O 2 ·- and ·OH and Type II ROS: 1 O 2 , showing significantly enhanced tumor phototoxicity in both normoxia and hypoxic tumor microenvironments. In vivo testing demonstrated that DTIR780 NPs was efficiently accumulated at the tumor site while significantly inhibiting tumor growth in a subcutaneous H22 tumor mouse model. In summary, this lysosome and mitochondria targeted DTIR780 NPs has great potential in clinically image-guided type I photodynamic therapy (PDT) and photothermal therapy (PTT), offering a novel avenue for developing high-performance type I PDT/PTT photosensitizers for the efficient treatment of hypoxia tumors.
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JOURNAL OF CONTROLLED RELEASE
·
2026
·
IF 12.4
Sheng Zhao,Yongyi Qu,Wei Chen,Ruotong Li,Jing Zhu,Yunyun Wu,Lunli Xiang,Lin Li,Hongrui Zhu,Jixi Zhang
Nanocomposite hydrogel enables spatial redox compartmentalization. • Nanoparticle-released neocuproine hijacks endogenous Cu(I) pools. • Mitochondrial Cu(I) redistribution induces immunogenic cuproptosis. • Extracellular antioxidant components buffer ROS to preserve immunity. • Preoperative in situ vaccination elicits durable immune memory. Cuproptosis-based in situ tumor vaccination provides a unique opportunity to transform tumor destruction into systemic antitumor immunity. However, the oxidative stress required for efficient tumor killing simultaneously disrupts immune competence through copper toxicity and immunosuppressive remodeling. Herein, we develop a spatial redox compartmentalized nanocomposite hydrogel platform to resolve this paradox by selectively amplifying intracellular cuproptosis while preserving the extracellular immune niche. Following peritumoral co-injection of gallic acid-grafted chitosan (CS-GA), dopamine-grafted oxidized hyaluronic acid (OHA-DA), and neocuproine-caffeic acid (NCP-CA) nanoparticles, the hydrogel establishes distinct redox microdomains for tumor eradication and immune protection. Specifically, NCP selectively exploits endogenous exchangeable Cu(I) pools and redirects copper toward mitochondria, where copper valence transformation and ferredoxin 1-mediated activation induce dihydrolipoamide S -acetyltransferase oligomerization, mitochondrial proteotoxic stress, and cuproptotic immunogenic cell death. Meanwhile, CA treatment was associated with increased AMPK phosphorylation and a 63.5% reduction in PD-L1 expression. Simultaneously, catechol-mediated redox cycling by CS-GA&OHA-DA continuously scavenges extracellular reactive oxygen species, alleviating oxidative immunosuppression and restoring T-cell viability to 94.4%. After 14 days of treatment, a substantial tumor regression was observed with a tumor volume reduction exceeding 64.2%. In a contralateral tumor rechallenge model, enhanced infiltration of CD8 + T-cells (40.8%) and CD8 + T EM -cells (26.4%) contributed to 75.6% inhibition of tumor recurrence and 93.3% suppression of metastasis. By exploiting endogenous copper resources rather than exogenous copper supplementation and spatially decoupling tumoricidal oxidation from immune-protective antioxidation, this hydrogel platform establishes a new approach for cuproptosis-based in situ vaccination, inducing durable antitumor immune memory and effectively preventing postoperative tumor recurrence and metastasis. Download: Download high-res image (299KB) Download: Download full-size image
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BIORESOURCE TECHNOLOGY
·
2026
·
IF 8.2
Zhongqi Dong,Yu Zhang,Qian Wu,En Yang,Jinping Yan
TtHSF2β-I regulates lignin transformation across multiple substrate levels. • Multi-scale framework links natural biomass to defined lignin systems. • TtHSF2β-I may relieve TtHSF2a-mediated repression by interfering with oligomerization. • TtHSF2β-I improves aromatic compound transformation and utilization. White-rot fungi are the primary biological decomposers of lignocellulosic biomass owing to their powerful lignin-modifying enzyme (LMEs) system. Here, we analyzed the regulatory role of TtHSF2β-I, an alternatively spliced variant of heat shock transcription factor (HSF) in Trametes trogii S0301, at three levels of substrate complexity: native poplar wood, synthetic lignocellulosic media, and specific lignin-derived monomers. Overexpression of TtHSF2β-I resulted in extensive disruption of poplar wood lignocellulose, whereas its deletion impaired fungal growth and substrate degradation. Transcriptomic analysis revealed that overexpression of TtHSF2β-I enhanced the expression of numerous genes encoding key lignocellulose-degrading enzymes. Using synthetic lignocellulose media and specific lignin-derived compounds, we further demonstrated that TtHSF2β-I controls the degradation and transformation of lignin-related compounds. Structural modeling and protein interaction assays suggested that TtHSF2β-I lacks direct DNA-binding activity, in contrast to TtHSF2α. Instead, it modulates transcription via protein–protein interactions, possibly interfering with TtHSF2α oligomerization to de-repress ligninolytic genes. In summary, our results indicate that alternative splicing plays a significant role in regulating ligninolysis in fungi. TtHSF2β-I, in particular, could be a useful target for engineering fungi with improved efficiency in biomass conversion or bioremediation. Download: Download high-res image (186KB) Download: Download full-size image
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INORGANIC CHEMISTRY
·
2026
·
IF 4.6
Pan Shifeng,Tong Hua,Li Haibo,Wang Yuansheng,Zhou Ziyang,Liu Wei,Ouyang Gangfeng
Currently, mainstream white light-emitting diodes (WLEDs)
fabricated
by combining a blue LED chip with Y 3 Al 5 O 12 :Ce 3+ yellow phosphor suffer from poor color rendering,
unstable color temperature, and blue light hazards due to the lack
of a red spectral component. In this study, a low-cost, easily synthesizable,
nontoxic, and highly stable red-emitting Cu(I)-based hybrid material, Cu 2 I 2 (2-et-3-mepz) (2-et-3-mepz = 2-ethyl-3-methylpyrazine),
is developed. This red-emitting material exhibits intense red emission
under ultraviolet/blue light excitation, with an emission peak at
628 nm and CIE chromaticity coordinates of (0.628, 0.372), which are
close to the standard red light. Experimental and theoretical analyses
reveal that its luminescence mechanism primarily originates from hybrid
metal/halide-to-ligand charge transfer ( 3 M/HLCT) and cluster-centered
( 3 CC) transitions. A WLED device fabricated by combining Cu 2 I 2 (2-et-3-mepz) with commercial blue and green phosphors
achieves an Ra value as high as 93.0. Furthermore, Cu 2 I 2 (2-et-3-mepz) also exhibits excellent scintillation performance
with a light yield of 12,647 photons MeV –1 . A flexible
scintillation screen prepared using this material achieves a spatial
resolution of 9.45 lp mm –1 , demonstrating its potential
application value in X-ray imaging. This work not only achieves high-performance
WLED devices but also provides a new strategy for developing low-cost,
environmentally friendly red-emitting materials.
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APPLIED CATALYSIS B-ENVIRONMENTAL
·
2026
·
IF 19.7
Ruirui Wang,Hasnain Nawaz,Linbo Huang,Sheng Zhong,Shuang Wei,Bin He,Ruixia Liu,Suojiang Zhang
Electrochemical CO 2 reduction to highly selective ethylene is particularly desirable yet challenging. The competition between methane and ethylene in the CO 2 RR process underscores the critical necessity of designing the catalyst for ethylene by suppressing the methane pathway. Herein, we introduced controllable Cu(II)/Cu(I) interfaces by regulating the ratio of ionic liquids (ILs) and H 2 O. The Cu x O-IL4 catalyst with main CuO/Cu 2 O structure, exhibits a high Faraday efficiency toward C 2 H 4 (∼54 %) and less CH 4 (∼1 %) under 250 mA·cm −2 current density at −1.18 V (vs RHE). Experimental and theoretical analyses demonstrate that Cu(I) sites favor CO bridge formation, while Cu(II) sites preferentially generate CO atop on Cu x O-IL4 catalysts. The Cu(II)/Cu(I) interface in Cu x O-IL4 facilitates the generation of CO bridge intermediates, thereby lowering the energy barrier for *CO dimerization and also preventing *CO hydrogenation to CH 4 formation, thus enhancing the selectivity of CO 2 -to-ethylene conversion. The study provides a novel approach for precisely controlling CO adsorption configurations to enhance CO 2 RR selectivity toward high-value multicarbon products formation.
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CHINESE JOURNAL OF CHEMISTRY
·
2026
·
IF 5.7
Shifeng Pan,Yuansheng Wang,Haibo Li,Ziyang Zhou,Zhennan Zhou,Wei Liu,Gangfeng Ouyang
Comprehensive Summary Traditional inorganic single-crystal scintillators face several adverse conditions, such as high cost, complex process and poor flexibility. To avoid these shortcomings, in this work, the low-cost benzyl bromide is used to efficiently synthesize the 0D organic-inorganic hybrid copper(I) halide compound Cu 5 I 4 Br 3 (bz-ted) 2 (Compound Y ) by the room-temperature solution method. The yields of the ligand (95%) and the scintillator (97%) are nearly 100%, and a single-batch production of 10.29 g is achieved, while the cost of the ligand raw material is drastically reduced. This material has an “all-in-one” (AIO) structure, with intramolecular π···π stacking interaction and Cu···Cu cuprophilic interactions together enhancing structural rigidity, resulting in its remarkable thermal, air, and light stability. Notably, Compound Y exhibits excellent yellow light-emitting properties with an outstanding photoluminescent quantum yield (PLQY = 90.66%) and an ultrahigh external quantum efficiency (EQY = 81.59%), which is investigated to be the emission from the self-trapped excitons (STEs). Moreover, it has favorable scintillation properties, with a low detection limit of 2.426 μGy air ·s −1 and a satisfactory spatial resolution of 17.7 lp·mm −1 . When blended with styrene-ethylene-butene-styrene (SEBS) to fabricate the flexible scintillation screen, it can withstand 200% stretching and 0°–90° bending, with no halo distortion in imaging. This work not only promotes the commercial application of AIO-structured materials, but also provides a new approach for the large-scale fabrication of low-cost and high-performance flexible X-ray scintillators.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Zhiyan Jiang,Junyao Liu,Caihua Shao,Siying Wang,Juan Xu,Ying Wang,Xiaoyu Lin,Haishun Xu,Xueqian Wu
Flavonoid compounds in Tetrastigma hemsleyanum Diels et Gilg ( T. hemsleyanum ) are essential for plant stress responses, yet their biosynthetic pathways and regulatory mechanisms remain poorly understood. This study identified and characterized two flavanone-3-hydroxylase ( F3H ) genes, Th F3H1 and Th F3H2, from T. hemsleyanum , which belong to the 2ODDs-DOXC28 subfamily. Tissue-specific expression analysis revealed that both genes are highly expressed in tuberous roots, with distinct stress response patterns observed. Sequence and phylogenetic analyses confirmed that these genes contain typical structural domains of the 2ODD family and are closely related to F3H proteins from various plant species. Subcellular localization studies indicated that Th F3H1 is predominantly cytoplasmic, while Th F3H2 is found both in the cytoplasm and the nucleus. In vitro enzymatic assays demonstrated that both enzymes catalyze the conversion of naringenin to dihydrokaempferol and small amounts of apigenin, with Th F3H2 exhibiting higher catalytic efficiency ( k cat / K m = 0.0093 s −1 ·μM −1 ). AlphaFold2 predictions and molecular docking analysis revealed the binding modes of Th F3H1 with itssubstrates. Site-directed mutagenesis further identified four key catalytic residues (Glu122, Lys196, Lys326, and Asp330). Furthermore, both genes were significantly upregulated under salt stress, and their heterologous expression enhanced the salt tolerance of Escherichia coli , underscoring their critical roles in plant stress responses. These findings enhance the understanding of plant F3H function and provide a molecular foundation for improving the quality and stress resistance of T. hemsleyanum .
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IEEE SENSORS JOURNAL
·
2026
·
IF 4.5
Chengzhang Han,Jinli Li,Zhenyu Wang,He Lv,Yusuf Suleiman Dambatta,Jingjie Dai
Cardiovascular diseases remain a leading cause of death worldwide, with acute myocardial infarction (AMI) accounting for a significant proportion of fatalities. The rapid and accurate detection of cardiac troponin I (cTnI) is critical for early diagnosis of AMI. We report an innovative, highly sensitive, solidly mounted resonator (SMR) biosensor based on a c-axis-tilted scandium-doped aluminum nitride (Sc0.1Al0.9N) film for label-free cTnI detection. The SMR device, fabricated by magnetron sputtering and incorporating an acoustic Bragg reflector, is designed to operate in the shear acoustic mode. The sensor demonstrates a quality factor of 2030 and an effective electromechanical coupling coefficient of 2.6%. Functionalized with a self-assembled monolayer and monoclonal antibodies, the sensor achieves a detection limit of 3 pg/mL for cTnI in serum, with a rapid response time of 100 s and high selectivity against common interferents. The device also exhibits significant potential for integration with CMOS electronics, supporting the development of miniaturized, point-of-care (POC) diagnostic systems for cardiovascular diseases.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Meiling Lian,Haoquan Wang,Liuxing Chen,Da Chen,Qi Yang,Xuezhi Qiao
Electrochemical sensing devices often suffer from biofouling due to nonspecific adsorption of biomolecules and microorganisms in complex biological matrices. To address this challenge, we developed an efficient antifouling and self-sterilizing sensing interface by grafting polyethylene glycol (PEG) onto two-dimensional Ti 3 C 2 T x MXene nanosheets for the accurate detection of cardiac troponin I (cTnI), a gold-standard biomarker for acute myocardial infarction (AMI). The Mn 2+ -intercalated Ti 3 C 2 T x (Mn-Ti 3 C 2 T x ) exhibited enhanced electrochemical activity in a three-electron oxygen reduction reaction (3e - ORR), enabling in situ self-sterilization by activating molecular oxygen to generate hydroxyl radicals (•OH), which demonstrated potent antibacterial effects against both Gram-negative and Gram-positive bacteria. Density functional theory calculations revealed that Mn doping in MXene significantly reduced the free energy barrier of *OOH intermediate, promoting H 2 O 2 formation and subsequent reduction to •OH. The PEGylated Mn-Ti 3 C 2 (PEG@Mn-Ti 3 C 2 T x ) composite combined the superior hydrophilicity of PEG for effective protein repulsion with the enhanced conductivity of Mn-Ti 3 C 2 T x , enabling sensitive biosensing. Critically, the fabricated biosensor demonstrated high specificity for cTnI against common interferents and achieved reliable detection in spiked serum samples. It exhibited a broad detection range from 0.1 pg mL -1 to 500 ng mL -1 with an ultralow limit of 0.03 pg mL -1 . This work presents a novel strategy for combating biofouling and paves the way for designing robust biosensors capable of accurate and long-term operation in clinically relevant environments, holding great promise for point-of-care diagnostics.
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MEDICAL ONCOLOGY
·
2025
·Vol. 42
·期 7
·1-12
·
IF 3.5
Tang Qing,Huang Kun,Li Mengjie,Zhao Ziqiao,Deng Yuanle,Zuo Yueqi,Wang Ningyu,Zhu Yongxia
Ovarian cancer is a deadly gynecological malignancy often diagnosed at an advanced stage, with few treatment options available. Polyphyllin I (PPI), the primary active ingredient of Paris polyphylla , has shown excellent anti-cancer activity against various cancers. However, studies on PPI use and ovarian cancer are limited, and the potential mechanisms remain unclear. This study explored the effects of anti-ovarian cancer and the potential mechanisms of action of PPI. Methods such as MTT, colony formation, three-dimensional spheroid formation, flow cytometry, and western blotting were employed to assess the anti-proliferative effects of PPI on different ovarian cancer cell lines. RNA sequencing was used to discover potential mechanisms by evaluating the effects of PPI on transcriptional signatures and identifying differences in gene expression patterns. The results indicate that PPI demonstrated dose- and time-dependent anti-proliferative effects on ovarian cancer cell lines. PPI also up-regulated 1518 genes and down-regulated 800 genes. The genes showed enrichment in the “apoptosis” gene sets, while exhibiting negative enrichment in the “cell cycle” and “Myc signaling pathway” gene sets. PPI caused cell cycle arrest at the G0/G1 phase by increasing the expression of p21 and p27 and inhibiting the activity of the CDK2/cyclin E complex. PPI triggered cell apoptosis, which was associated with elevated cellular reactive oxygen species and mitochondrial membrane potential depolarization. Finally, PPI reduced c-Myc protein levels and affected mRNA levels of c-Myc pathway-related genes. PPI effectively suppressed ovarian cancer cell proliferation, indicating its potential as a treatment option for ovarian cancer.
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