CHEMOSPHERE
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2024
·
IF 8.1
Jie Li,Haoran Song,Tao Luo,Yan Cao,Linfeng Zhang,Qun Zhao,Zhanchao Li,Xuewei Hu,Junjie Gu,Senlin Tian
Exposure to ozone (O 3 ) and nitrogen dioxide (NO 2 ) are related to pulmonary dysfunctions and various lung diseases, but the underlying biochemical mechanisms remain uncertain. Herein, the effect of inhalable oxidizing gas pollutants on the pulmonary surfactant (PS, extracted from porcine lungs), a mixture of active lipids and proteins that plays an important role in maintaining normal respiratory mechanics , is investigated in terms of the interfacial chemistry using in-vitro experiments; and the oxidative stress induced by oxidizing gases in the simulated lung fluid (SLF) supplemented with the PS is explored. The results showed that O 3 and NO 2 individually increased the surface tension of the PS and reduced its foaming ability; this was accompanied by the surface pressure-area isotherms of the PS monolayers shifting toward lower molecular areas, with O 3 exhibiting more severe effects than NO 2 . Moreover, both O 3 and NO 2 produced reactive oxygen species (ROS) resulting in lipid peroxidation and protein damage to the PS. The formation of superoxide radicals (O 2 •– ) was correlated with the decomposition of O 3 and the reactions of O 3 and NO 2 with antioxidants in the SLF. These radicals, in the presence of antioxidants, led to the formation of hydrogen peroxide and hydroxyl radicals ( • OH). Additionally, the direct oxidation of unsaturated lipids by O 3 and NO 2 further caused an increase in the ROS content. This change in the ROS chemistry and increased • OH production tentatively explain how inhalable oxidizing gases lead to oxidative stress and adverse health effects. In summary, our results indicated that inhaled O 3 and NO 2 exposure can significantly alter the interfacial properties of the PS, oxidize its active ingredients, and induce ROS formation in the SLF. The results of this study provide a basis for the elucidation of the potential hazards of inhaled oxidizing gas pollutants in the human respiratory system.
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CHEMICAL ENGINEERING JOURNAL
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2024
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IF 13.3
Yanghan Sun,Yajun Xie,Hanyan Zou,Yuhua Chen,Zhuo Wen,Qun Liang,Xinyu Peng,Jiuxi Sui,Jiajia Chen,Yuanke He,Yuxuan Wu,Lizhong Guo,Guixue Wang,Guangchao Zang,Yuchan Zhang
Conductive hydrogel films, as integral elements of flexible sensors, hold promising applications across diverse fields. However, their cost-effective and straightforward production, ensuring both excellent mechanical properties and conductivity, remains a formidable challenge. In our research, we developed a multifunctional conductive hydrogel film using a freeze-thaw cycling-annealing method. That process applied freeze-thaw cycling to reorganize the hydrogel’s cross-linking structure and improve the polyvinyl alcohol molecules’ crystallinity through annealing. The thickness of the fabricated film was approximately 100 μm, and the limit of detection and sensitivity for glucose were 2.82 μM and 117.6 μA /mM*cm 2 respectively, and the sensitivity factor during the bending process was 2.61, which demonstrated excellent biocompatibility, mechanical properties, and electrochemical properties. It also facilitates wireless, real-time electrophysiological signal monitoring and sweat glucose tracking in the human body. In summary, the freeze-thaw cycling/annealing approach simplified and accelerated hydrogel film preparation. This development is poised to resolve the intricacies of existing hydrogel film production methods and broaden the application scope of such films in wearable devices.
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ANALYTICAL CHEMISTRY
·
2024
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IF 6.7
Hao Liu,Jiang Ren,Lebao Mao,Chengyi Xiong,Xiuhua Zhang,Shengfu Wang,Wei-Hua Huang,Miao-Miao Chen
Hydrogen peroxide (H2O2) levels play a vital role in redox regulation and maintaining the physiological balance of living cells, especially in cell mechanotransduction. Despite the achievements on strain-induced cellular H2O2 monitoring, the applied voltage for H2O2 electrooxidation possibly gave rise to an abnormal expression and inadequate accuracy, which was still an inescapable concern. Hence, we decorated an interlaced CuO@TiO2 nanowires (NWs) semiconductor meshwork onto a polydimethylsiloxane film-supported gold nanotubes substrate (Au NTs/PDMS) to construct a flexible photoelectrochemical (PEC) sensing platform. Under white light irradiation, CuO@TiO2 NWs synergistically exhibited great stretchability and the PEC platform enabled stable photocurrent responses from the reduction of H2O2 even during mechanical deformation. Moreover, the admirable biocompatibility and an almost negligible open circuit voltage of +0.18 V for the CuO@TiO2 NWs/Au NTs/PDMS sensor guaranteed human umbilical vein endothelial cells (HUVECs) adhesion tightly thereon even under continuous illumination for 30 min. Finally, the as-proposed stretchable PEC sensor achieved sensitive and true-to-life monitoring of transient H2O2 release during HUVECs deformation, in which H2O2 release was positively correlated to mechanical strains. This investigation opens a new shade path on in situ cellular sensing and meanwhile greatly expands the application mode of the PEC approach.
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Nano-Micro Letters
·
2024
·Vol. 16
·期 1
·1-15
·
IF 31.6
Pan Yexin,Yu Ruohan,Jiang Yalong,Zhong Haosong,Yuan Qiaoyaxiao,Lee Connie Kong Wai,Yang Rongliang,Chen Siyu,Chen Yi,Poon Wing Yan,Li Mitch Guijun
Electronic waste (e-waste) and diabetes are global challenges to modern societies. However, solving these two challenges together has been challenging until now. Herein, we propose a laser-induced transfer method to fabricate portable glucose sensors by recycling copper from e-waste. We bring up a laser-induced full-automatic fabrication method for synthesizing continuous heterogeneous Cu x O (h-Cu x O) nano-skeletons electrode for glucose sensing, offering rapid (< 1 min), clean, air-compatible, and continuous fabrication, applicable to a wide range of Cu-containing substrates. Leveraging this approach, h-Cu x O nano-skeletons, with an inner core predominantly composed of Cu 2 O with lower oxygen content, juxtaposed with an outer layer rich in amorphous Cu x O (a-Cu x O) with higher oxygen content, are derived from discarded printed circuit boards. When employed in glucose detection, the h-Cu x O nano-skeletons undergo a structural evolution process, transitioning into rigid Cu 2 O@CuO nano-skeletons prompted by electrochemical activation. This transformation yields exceptional glucose-sensing performance (sensitivity: 9.893 mA mM −1 cm −2 ; detection limit: 0.34 μM), outperforming most previously reported glucose sensors. Density functional theory analysis elucidates that the heterogeneous structure facilitates gluconolactone desorption. This glucose detection device has also been downsized to optimize its scalability and portability for convenient integration into people’s everyday lives.
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ANALYTICAL CHEMISTRY
·
2025
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IF 6.7
Hui Dong,Weitian Chen,Ke Xu,Linlin Zheng,Bingyu Wei,Ruiyu Liu,Jingru Yang,Tao Wang,Yanli Zhou,Yintang Zhang,Maotian Xu
Parkinson’s disease (PD) is a progressive neurodegenerative disorder characterized by the loss of dopaminergic neurons and the accumulation of alpha-synuclein. Glutathione (GSH), a key antioxidant, is significantly depleted in PD patients. This study presents a dual-mode detection strategy for selectively determining GSH using a single probe. A series of “turn-on” electrochemical and fluorescent probes were developed, with resorufin (Re) serving as the reporting unit and featuring specific GSH recognition sites. Among these, the 7-(3,5-dinitrophenoxy)-3H-phenoxazin-3-one (Re-DNP) probe was selected for its high selectivity as both a fluorescent and electrochemical probe. Its response to GSH was superior in comparison to that observed for hydrogen sulfide (H2S) and cysteine (Cys). For electrochemical detection using screen-printed carbon electrode (SPCE)/carbon nanotube (CNT) modified electrodes, the detection limit for GSH was 5 μM, with a linear range of 25–500 μM. In fluorescence detection, the probe produced a 78-fold increase in emission at 630 nm in the presence of GSH, with a strong linear correlation between fluorescence intensity and GSH concentration in the range of 10–700 μM, and a detection limit of 2 μM. When applied to real clinical serum samples, the probe demonstrated significantly lower GSH levels in both PD mice and human patients compared to healthy controls. This dual-mode detection method provides a sensitive and accurate tool for GSH detection, with potential applications in understanding GSH’s role in PD and related neurodegenerative diseases.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-15
·
IF 15.7
Liu Lu,Li Xiurong,Chen Yongxian,Gao Junbin,Jiang Yuejun,Ye Yicheng,Wang Peng,Peng Fei,Tu Yingfeng
Uricase (UOx)-based gout treatments are generally limited due in part to the accumulation of H 2 O 2 in an arthrosis environment characterized by a sluggish metabolism. Here we develop a self-cascade nanomotor with high efficiency toward simultaneous uric acid (UA) degradation and H 2 O 2 elimination on the basis of UOx and sodium citrate loading in amine functionalized hollow mesoporous silica nanoparticles (AHMSNs). Due to the inherent asymmetry of AHMSNs, the developed nanomotors can be actuated by ionic diffusiophoresis induced by the enzymatic UA degradation, thus enlarging the diffusion range within the joint cavity. H 2 O 2 generated during UA decomposition can be simultaneously eliminated by the loaded sodium citrate, resulting in a considerable improvement in mammalian cell viability. In vivo studies display powerful therapeutic effect in gouty models using this self-cascade nanomotor system. These results present the potential of nanomotors as active therapeutic agents for gout, providing a perspective on the application of enzyme-driven micro/nanomotors.
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BIOSENSORS & BIOELECTRONICS
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2024
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IF 10.7
Chen Ye,Diwei Shi,Yangguang Zhu,Peizheng Shi,Ningbin Zhao,Zhuang Sun,Zhe Zhang,Diming Zhang,Yaokang Lv,Wenqi Wu,Jiancheng Yu,Hassan Karimi-Maleh,He Li,Li Fu,Nan Jiang,Juewen Liu,Cheng-Te Lin
Simultaneous monitoring of key metabolites like dopamine, ascorbic acid, and uric acid is essential for early disease diagnosis and evaluating treatment. Electrochemical techniques are increasingly used for precise, point-of-care testing (POCT) of these metabolites. Herein, a sample pretreatment method called effervescent solid-phase extraction (ESPE) was proposed for efficient enrichment of trace analytes for electrochemical detection. In an ESPE process, effervescent tablets made of gold nanoparticle-decorated graphene oxide (Au/GO) were first self-dispersed in a test solution to promote the enrichment of analytes on the Au/GO adsorbents, followed by the addition of flocculant effervescent tablets to cause Au/GO sheets to form self-assembled aggregates, which then can be efficiently collected by the foam electrodes. The entire sample pretreatment process operates without external power and takes only 5 min. With the assistance of the ESPE method, our electrochemical sensors achieve an ultralow detection limit for dopamine, ascorbic acid, and uric acid of 80 pM, 1.8 nM, and 460 pM, respectively, which are two to three orders of magnitude lower than the results obtained by the drop casting technique. The enhancement mechanism of our approach is based on increasing the contact probability with analytes through dynamic dispersion of the Au/GO adsorbents, in contrast to the static diffusion mechanism relied on Brownian motion. We also show that combining the ESPE solution kit with a portable micro-electrochemical workstation can attain the same detection level as HPLC in real urine samples. The proposed ESPE approach holds great promise for POCT applications in 2D material-based biosensors.
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JOURNAL OF ALLOYS AND COMPOUNDS
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2024
·
IF 5.8
Jingjing Liu,Liuwei Shi,Yu Shi,Qi Zeng,Yajie Yang
Co-ZIF is widely believed as a promising material which shows potential in MOFs based enzyme-free sensors. However, the research of Co-ZIF in glucose sensor filed has been hampered, due to its poor electronic conductivity and slow ion diffusion caused by closed rhombohedral dodecahedron structure. In this work, the obtained Co 0.95 Fe 0.05 -ZIF presents highly opened nanosheet structure on CC, resulting in rich open active sites and ions moving channels. Compared with other reported ZIFs, the Co 0.95 Fe 0.05 -ZIF/CC exhibits excellent sensing performance with a high sensitivity of 3232.30 μA mM −1 cm −2 in the range of 0.20–1.00 mM and a low detection limit of less than 5 μM, due to its highly opened nanosheet structure. The Co 0.95 Fe 0.05 -ZIF/CC also shows excellent selectivity, repeatability and reproducibility for glucose detection. Furthermore, the variation trend of test results measured by this saliva sensor without any sample pre-treatment, is consistent with that of commercial blood glucose meter, indicating its potential in real applications. This synthesis strategy also provides a new direction of structure construction for high performance MOFs in enzyme-free glucose sensor field.
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TALANTA
·
2024
·
IF 5.6
Yilin Wang,Xiaoyun Zhang,Maole Wei,Huigang Kang,Zhenqiang Zhang,Xiudan Wang,Cuiping Ma
Hypoxanthine (Hx), produced by adenosine triphosphate (ATP) metabolism, is a valuable indicator that determines the quality and degradation status of meat products and is also an important biochemical marker to certain diseases such as gout. The rapid emergence of paper-based enzyme biosensors has already revolutionized its on-site determination. But it is still limited by the complex patterning and fabrication, unstable enzyme and uneven coloration. This work aims to develop an eco-friendly method to construct engineered paper microfluidic, which seeks to produce reaction and non-reaction zones without any patterning procedure. Chito-oligosaccharide (COS), derived from shrimp shells, was used to modify nitrocellulose membranes and immobilize xanthine oxidase (XOD) and chromogenic agent of nitro blue tetrazolium chloride (NBT). After modification, micro fluids could converge into the modification area and Hx could be detected by XOD-catalyzed conversion. Due to the positively charged cationic basic properties of COS, the enzyme storage stability and the color homogeneity could be greatly strengthened through the electrostatic attraction between COS and XOD and formazan product. The detection limit (LOD) is 2.30 μM; the linear range is 0.05–0.35 mM; the complete test time can be as short as 5 min. The COS-based biosensor shows high specificity and can be used directly for Hx in complex samples such as fish and shrimp samples, and different broths. This biosensor is eco-friendly, nontechnical, economical and therefore a compelling platform for on-site or home-based detection of food freshness.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Qinghao Xiong,Shouhui Chen,Longsheng Pei,Jianming Liu,Yinan Yang,Meilin Lu,Yonghai Song
Covalent-organic frameworks (COF) and metal–organic frameworks (MOF) were promising precursors for porous carbon-based materials. Calcining individual COF or MOF is usually unfavorable when preparing electrochemical active materials that combine sensitivity, stability, and selectivity. In this work, calcining a core–shell structural Ni-MOF@COF TAPB-DVA prepared a yolk-shell structural composite (YS-Ni@NC), in which the N-doped carbon shell (NC) encapsulated the Ni nanoparticles yolk (NiNPs). A highly selective dopamine electrochemical sensor (YS-Ni@NC/GCE) was developed based on the YS-Ni@NC modified glassy carbon electrode (GCE). The NiNPs yolk in the derivates provided excellent electrocatalytic activity, and the N-doped carbon shell improved the composite conductivity, enhanced the structural stability, and reduced the nonspecific adsorption of uric acid and ascorbic acid through electrostatic repulsion force. The YS-Ni@NC/GCE electrode exhibited higher selectivity for dopamine than the control electrodes in the presence of uric acid and ascorbic acid due to the electrostatic interaction between YS-Ni@NC and the small molecules. At the optimal experimental conditions, the DA sensor showed a linear concentration range of 0.136∼70.0 μM with a detection limit of 0.041 μM. In addition, the electrochemical performance of the sensor was stable when stored at room temperature for 30 days. Preparation of the yolk-shell structural MOF@COF derivates provided a new approach for fabricating highly selective non-enzymatic dopamine electrochemical sensors.
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Surfaces and Interfaces
·
2025
·
IF 6.3
Pengcheng Jiao,Xiutao Yang,Yuying Wang,Hongwen Yu,Ying Zeng,Guopeng Zhu,Chunhui Wang,Deming Han
Rational design on the microstructure and chemical composition of transition metal-based electrode materials is critical for boosting electrochemical glucose detection. In this work, the hollow heterostructured CuO x @Co 3 O 4 nanotube arrays on Cu foam (CuO x @Co 3 O 4 NTAs/CF) were fabricated. Coupling Co 3 O 4 nanoparticles on CuO x nanotube modulates the electronic structure by heterostructure, enhancing charge transfer during electrocatalytic glucose oxidation. Moreover, the unique hierarchical tubular core-shell structure guarantees abundant well-distributed active sites and rapid pathways for glucose and electrolyte diffusion. Beneficial from the advanced structure, the sensor fabricated with CuO x @Co 3 O 4 NTAs/CF possesses a high sensitivity of 23347.4 μA mM⁻¹ cm⁻² at linear range of 10-527 μM and low limit of detection of 0.56 μM (S/N=3). In addition, the outstanding selectivity, reproducibility and long-term stability (94.5 % for 30 days) as well as good recovery rate in artificial saliva, sweat, and human serum samples are also accessible advantages for our fabricated sensor. The excellent sensing performance of CuO x @Co 3 O 4 NTAs/CF demonstrates its potential application for electrochemical glucose detection.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Yuxuan Zhu,Jing Qian,Ke Xu,Wanru Ouyang,Juan Yang,Nianjun Yang
Monitoring salivary glucose has gained increased attention as a non-invasive approach to prevent and diagnose diabetes. Since the glucose concentration in saliva is lower than that in blood, the development of effective electrocatalysts for highly sensitive glucose detection is thus of great significance. In this study, a hollow nanocage from NiCo-layered double hydroxides (LDH) modified with multi-walled carbon nanotubes (MWCNTs) is designed and further employed as the sensing material to construct a novel non-enzymatic glucose electrochemical sensor with high sensitivity. It is synthesized by utilizing pre-synthesized zeolitic imidazolate framework (ZIF-67)/MWCNTs as a sacrificial template and subsequently conducting a simple solvent-thermal reaction with Ni(NO 3 ) 2 . Such a hollow nanocage preserves the polyhedral framework structure of the used precursor, where the hollow frame-like structure of NiCo-LDH offers accessible catalytic sites, and MWCNTs provides good conductivity. Their combination brings in a bridging effect between MWCNTs and NiCo-LDH, further leading to a large electrochemical active area and excellent catalytic activity of this hollow nanocage. The constructed sensor exhibits a detection limit as low as 0.03 μM for the glucose detection as well as wide linear ranges of 0.1–3000 μM and 3000–9231.8 μM, corresponding to the sensitivity of 2.55 and 1.15 μA mM −1 cm −2 , respectively. Moreover, this sensor enables the tracking of glucose concentration change in salivary before and after food intake. This work offers new highly sensitive sensing materials and potentially valuable approaches for non-invasive glucose detection.
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