MICROCHEMICAL JOURNAL
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2020
·
IF 3.594
Na Guo,Yong-Peng Zou,Hong-Kun Li,Ping Kou,Zhi-Ming Liu,Yu-Jie Fu
Conventional approaches for extraction and enrichment of phytochemicals from plant matrix have many drawbacks such as high organic solvent consumption, low extraction yields, time-consuming and large labor for the enrichment process. In the present study, ultrasound-assisted deep eutectic solvents extraction (UAE-DESs) was developed for extraction and determination of the linarin from Chrysanthemum indicum L. flower (CIF). And a feasible strategy was performed to separate and recover the linarin from denatured DESs using AB-8 macroporous resins as adsorbents. Under the optimal extraction conditions, the maximum linarin extraction yield reached 14.23 mg/g with liquid–solid ratio 32 mL/g, extraction power 340 w, extraction time 32 min and deep eutectic solvent of choline chloride-ethylene glycol with the molar ratio 1:2 and water content 30% (v/v) as a extraction system, which was 1.21 times higher than 80% ethanol extraction. After macroporous resins enrichment, the recovery efficiency of linarin reached 81.55%. In addition, the DESs could be regenerated for extraction process, and macroporous resins could be further used for enrichment process with desired performance. This study provided an effective and convenient extraction and recovery method for linarin determination from CIF which was also applicable to other natural plants.
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DRUG DELIVERY
·
2020
·
IF 4.902
Tan Yang,Jianfang Feng,Qian Zhang,Wei Wu,Hailan Mo,Lanzhen Huang,Wei Zhang
A delivery system based on l-carnitine (LC) conjugated chitosan (CS)-stearic acid polymeric micelles has been developed for improving the oral bioavailability of paclitaxel (PTX) through targeting intestinal organic cation/carnitine transporter 2 (OCTN2). Stearic acid grafted chitosan (CS-SA), as micelle skeleton material, was synthesized by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-mediated coupling reaction. The PTX-loaded micelles were prepared by solvent evaporation-hydration method, and the ligand LC was conjugated onto the micelle surface by anchoring its derivative stearoyl group to the lipophilic core of micelle. The modified polymeric micelles showed regular spherical shapes with small particle size of 157.1 ± 5.2 nm and high drug loading capacity of 15.96 ± 0.20 wt%, and the micelle stability in water was supported by low critical micelle concentration of 14.31 ± 0.21 μg/ml. The drug-loaded micelles presented a slow and incomplete in vitro release, and the pharmacokinetic studies indicated the micelle carriers increased the relative bioavailability of PTX to 165.8% against the commercial formulation. The enhancement effect on intestinal absorption was also confirmed by the intracellular uptake of Caco-2 cells. The proposed micelle carrier system manifested a prospective tool for oral drug delivery.
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RSC Advances
·
2020
·Vol. 10
·期 24
·14218-14224
·
IF 3.119
Zai-Yu Li,Dan-Yang Gao,Zhi-Yong Wu,Shuang Zhao
Herein multi-walled carbon nanotubes (MWCNTs) were processed by ultrasonication and freeze-drying method. The morphology of the processed MWCNTs was examined by scanning electron microscopy. An original electrochemical sensor for the simultaneous detection of levodapa (LD), paracetamol (PA) and L-tyrosine (Tyr) was developed by dropcasting a mixture of processed MWCNTs and Nafion on a glassy carbon electrode. The as-prepared sensor was studied by cyclic voltammetry and differential pulse voltammetry. The peak currents of LD, PA and Tyr significantly increased compared to those obtained at bare glassy carbon electrodes or unprocessed MWCNTs modified electrodes. The peaks of LD, PA and Tyr were well-defined and obviously separated from each other. The linear ranges for detection of LD, PA and Tyr were 2.0–300.0, 2.0–180.0, and 2.0–120.0 μM, with a detection limit of 0.6, 0.5 and 0.8 μM (S/N = 3), respectively. Finally, the sensor was applied to detect LD, PA and Tyr in serum samples, and the results were satisfactory.
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PLANT BIOLOGY
·
2020
·Vol. 22
·期 4
·679-690
·
IF 2.167
A. Sami,F. A. Shah,M. Abdullah,X. Zhou,Y. Yan,Z. Zhu,K. Zhou
Melatonin has emerged as an essential molecule in plants, due to its role in defence against metal toxicity. Aluminium (Al) and cadmium (Cd) toxicity inhibit rapeseed seedling growth. In this study, we applied different doses of melatonin (50 and 100 µ m ) to alleviate Al (25 µ m ) and Cd (25 µ m ) stress in rapeseed seedlings. Results show that Al and Cd caused toxicity in rapeseed seedling, as evidenced by a decrease in height, biomass and antioxidant enzyme activity. Melatonin increased the expression of melatonin biosynthesis-related Brassica napus genes for caffeic acid O-methyl transferase ( BnCOMT ) under Al and Cd stress. The genes BnCOMT-1 , BnCOMT-5 and BnCOMT-8 showed up-regulated expression, while BnCOMT-4 and BnCOMT-6 were down-regulated during incubation in water. Melatonin application increased the germination rate, shoot length, root length, fresh and dry weight of seedlings. Melatonin supplementation under Al and Cd stress increased superoxide dismutase, catalase, peroxidase, ascorbate peroxidase, proline, chlorophyll and anthocyanin content, as well as photosynthesis rate. Both Cd and Al treatments significantly increased hydrogen peroxide and malondialdehyde levels in rapeseed seedlings, which were strictly counterbalanced by melatonin. Analysis of Cd and Al in different subcellular compartments showed that melatonin enhanced cell wall and soluble fractions, but reduced the vacuolar and organelle fractions in Al- and Cd-treated seedlings. These results suggest that melatonin-induced improvements in antioxidant potential, biomass, photosynthesis rate and successive Cd and Al sequestration play a pivotal role in plant tolerance to Al and Cd stress. This mechanism may have potential implications in safe food production.
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ANALYTICAL LETTERS
·
2021
·
IF 2.329
Zhipeng Su,Fanyan Ye,Kaiyu He,Tao Yang,Wang Li,Jiali Ren
The sensitive determination of pesticides constitutes an important aspect in ensuring food safety. Due to the disadvantages of traditional methods, such as low sensitivity, complex operation, and high cost, the development of an efficient and economical biosensor to detect pesticide residues is required. In this work, the dipeptide glycyl-L-histidine (GH) and divalent copper ions (Cu2+) were employed as bioorganic linkers and metal ions in the synthesis of the peptide-based metal organic framework (MOF) material GH-Cu BioMOF. Because of its properties of fluorescence quenching and adsorption of nucleic acids, GH-Cu BioMOF was used to develop a distinctive aptamer based method for the determination of pesticide residues in food. The GH-Cu BioMOF-based aptasensor was used for acetamiprid and demonstrated a linear response in the range from 0.15 to 3.00 µM with a detection limit of 137 nM, verifying the feasibility of this method for pesticide determination in food.
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NATURE BIOTECHNOLOGY
·
2021
·Vol. 39
·期 12
·1548-1555
·
IF 54.908
Fan Chuyao,Deng Qiang,Zhu Ting F.
Natural DNA is exquisitely evolved to store genetic information. The chirally inverted l -DNA, possessing the same informational capacity but resistant to biodegradation, may serve as a robust, bioorthogonal information repository. Here we chemically synthesize a 90-kDa high-fidelity mirror-image Pfu DNA polymerase that enables accurate assembly of a kilobase-sized mirror-image gene. We use the polymerase to encode in l -DNA an 1860 paragraph by Louis Pasteur that first proposed a mirror-image world of biology. We realize chiral steganography by embedding a chimeric d -DNA/ l -DNA key molecule in a d -DNA storage library, which conveys a false or secret message depending on the chirality of reading. Furthermore, we show that a trace amount of an l -DNA barcode preserved in water from a local pond remains amplifiable and sequenceable for 1 year, whereas a d -DNA barcode under the same conditions could not be amplified after 1 day. These next-generation mirror-image molecular tools may transform the development of advanced mirror-image biology systems and pave the way for the realization of the mirror-image central dogma and exploration of their applications.
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POLYMERS FOR ADVANCED TECHNOLOGIES
·
2021
·Vol. 33
·期 2
·534-545
·
IF 3.665
Chen Cheng,Yanling Lu,Junfeng Cai,Jun Yan,Shaojie Li,Shiguo Du
To improve the flame retardance and compatibility of ammonium polyphosphate (APP) via an environmentally friendly method, l -lysine (Lys), a bio-based amino acid, was selected as the surface-modifier for APP in this work. Lys-modified APP (L-APP) was prepared through the cation exchange reaction, and its morphology and chemical composition were characterized comprehensively. The characterization results demonstrated the fabrication of Lys shell structure on the surface of APP. Additionally, the thermogravimetric analysis illustrated that the char-forming ability of APP was significantly improved by the Lys shell structure. The flame retardance of APP and L-APP on epoxy resin (EP) was evaluated by the limiting oxygen index (LOI), vertical burning (UL-94), and cone calorimeter tests (CCT). The results showed that EP blending with 15 wt% L-APP (EP/L-APP15) passed V-0 rating, and got an LOI value of 31.7%. In the CCT, compared to the pristine EP, the peak heat release rate and total smoke production of EP/L-APP15 were decreased by 77.6% and 47.1%, respectively. It was confirmed that the good flame retardance and smoke suppression performance of L-APP can be attributed to the phosphorus-containing intumescent char residuals formed during combustion process. The tensile strain tests were conducted to assess the mechanical properties of EP composites. The results demonstrated that compared to pristine APP, L-APP possessed weakened impact on the tensile strain property of EP, which was beneficial to the practical application.
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Biomaterials Science
·
2021
·Vol. 9
·期 22
·7432-7443
·
IF 6.843
Chuyi Han,Qinghua Yu,Junhao Jiang,Xianming Zhang,Fan Wang,Mingyue Jiang,Ruihong Yu,Tao Deng,Chao Yu
Nitric oxide (NO) is a short-lived, bioactive gas that has been found to have affinitive effects on cardiovascular diseases as well as cancer biology, while NO deficiency may cause serious pathological responses. The existing chemically-synthesized NO donors have inevitable systemic toxicity and cannot be released adaptively. Hence, L-arginine, an endogenous NO precursor, merits investigation as a natural efficient NO donor. Herein, we designed amino acid-doped L-arginine CDs-based bioenzyme-responsive NO donors, which could adaptively replenish NO/ONOO− in response to different microenvironments. Our results indicated the mechanism of the NO/ONOO− supplementation of L-arginine-based CDs and their potential for nonpharmaceutical gas-involving theranostics for the first time.
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Molecular Catalysis
·
2021
·
IF 5.062
Wei Luo,Jiagui Hu,Jiapeng Lu,Huili Zhang,Xiaoping Wang,Yuantao Liu,Liqing Dong,Xiaobin Yu
ω-transaminase can mediate the asymmetric synthesis of chiral amines from aldehydes and ketones , which has important value in the synthesis of pharmaceutical intermediates. A novel ω-transaminase derived from Paracoccus pantotrophus ( Pp TA) was obtained and cloned in E. coli BL21(DE3) for expression. The enzyme has high activity for 2-ketobutyric acid and benzylamine , as well as for aromatic compounds with side chains longer than ethyl aliphatic hydrocarbons . Molecular simulation showed that the S-pocket in the active center is larger than those of other ω-transaminases. Thereafter, a whole-cell catalytic system was designed to prepare l -2-aminobutyric acid by cascading Pp TA and other enzymes. By using several strategies (regulation of RBS intensity, by-product decomposition and cofactor self-sufficiency), whole-cell cascade biocatalysis showed a high ee value (> 99%) and high yield (71%) in one pot reaction . This study therefore proposes an efficient biocatalyst for the synthesis of unnatural amino acids with the participation of ω-transaminase.
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Waste and Biomass Valorization
·
2020
·Vol. 11
·期 12
·6967-6979
·
IF 2.851
Yao Yuan,Hou Hongying,Liu Xianxi,Meng Kun,Qiu Jinxu,Wang Lei,Zhu Jing
Abstract Abstract In this work, copper l -phenylalanine (abbreviated as Cu(L-Phe) 2 ) complex was extracted from the waste acid Cu-printed-circuit-board (Cu-PCB) etchant via a one-step facile liquid phase precipitate reaction for the first time. The corresponding application feasibility in lithium-ion battery (LIB) and the electrochemical Li-storage mechanism were also explored. The resultant Cu(L-Phe) 2 complex displayed a nanosheet-like morphology with 200 nm in thickness. When it was used as the anode active material of LIB, the reversible discharge specific capacities were 231.1 mAh/g at 100 mA/g and 47.2 mAh/g at 5 A/g, respectively. Furthermore, with the help of surfactant sodium dodecyl sulfate (SDS), Cu(L-Phe) 2 complex nanosheets rearranged into the orderly flower-like cluster with a diameter of 13 μm; correspondingly, the reversible discharge specific capacities also increased to about 278.8 mAh/g and 108.8 mAh/g, respectively, implying higher reversibility and cycle performance. Seemingly, orderly flower-like nanosheets clusters with the hierarchical structure may facilitate to modify the electrochemical Li-storage capacity of Cu(L-Phe) 2 complex anode. Clearly, the satisfactory results may open a new path to develop the circular economy mode of acid waste Cu-PCB etchant, which is very important for environmental protection and sustainable development. Graphic Abstract
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JOURNAL OF POLYMER RESEARCH
·
2019
·Vol. 26
·期 12
·1-9
·
IF 1.53
Luo Chun Hui,Sun Xin Xin,Wang Feng,Wei Ning,Luo Fa Liang
Environmentally responsive hydrogels are widely used in various applications. Facile synthesis of multiply stimuli-responsive hydrogels is necessary. Herein, a triple pH-, thermo- and ion-sensitive hydrogel, shorted as HSP, was synthesized by aqueous radical polymerization using L-serinyl acrylate as a monomer and PEG 180 DMA as a crosslinker. The compositions and microstructures of HSP are characterized by FT-IR, 1 H NMR and SEM. The swelling ratio of HSP is the lowest at pH = 3.0 while increases sharply far away from this pH value. Meanwhile, HSP expands with elevated temperatures or ionic strengths, and reaches a plateau when they above 60 °C or 3.0 mol/L, respectively. In addition, these swelling processes are reversible under alternative changing in extern stimuli, and these cycles can be repeated at least 5 times. Furthermore, the release of sodium salicylate can be easily mediated by pH values, temperatures and ion concentrations, all above indicating that HSP is a promising material for controlled drug delivery.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Hongwei Pan,Zengwen Cao,Yunjing Chen,Xiangyu Wang,Shiling Jia,Huili Yang,Huiliang Zhang,Lisong Dong
A comparative study on interfacial crystallization of poly( l -lactic acid) (PLLA) with different stereoregularity (PLLA2003D 96.0%, PLLA4032D 98.5%, and PLLA290 99.2%) surrounding macroscopic basalt fibers (BF) has been carried out in polymer composites. The effect of stereoregularity on the interface crystallization was systemically studied by microstructure. From polarized optical microscopy (POM) analysis, it was found that the transcrystallinity of PLLA was influenced by temperature. Moreover, stereoregularity significantly affects the crystallization kinetics . It was also found that increased molecular mobility providing faster orientation with high stereoregularity and low molecular weight. It might be the reason for the observation that the PLLA290 samples crystallized more rapidly than the PLLA4032D samples. Appropriate molecular stereoregularity exhibits a clear increase in extensional stress that is directly correlated with the crystalline orientation of the crystallization samples. The mechanism of formation of transcrystallinity underwent the process of “a mismatch in thermal expansion coefficients” with the extensional stress.
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危险品化学品经营许可证（带存储）