OPTICAL MATERIALS
·
2019
·
IF 2.687
Chunmei Huang,Ying Qian
For highly sensitive and selective detection of ClO − over other reactive oxygen species (ROS) and anions, a fluorescent probe MN-BODIPY by introducing a morpholine group into the 8-position of BODIPY which could be used for targeting lysosome and improving water solubility was designed and synthesized. As a result of the C=N isomerization mechanism at 2-position of BODIPY, the probe alone generated extremely weak fluorescence. However, in the presence of ClO − , the probe exhibited a dramatic enhancement of fluorescence intensity by a 29-fold increase at 530 nm accompanying with quantum yield changing from 0.03 to 0.25. Accordingly, the probe displayed fast response for ClO − within 60 s. The limit of detection (LOD) for ClO − was calculated as 16.5 nM from the titration experiments, which is lower than most previously reported ClO − -selective probes. Moreover, MN-BODIPY had been successfully employed for monitoring exogenous and endogenous hypochlorous in MCF-7 cells with satisfying results, perhaps it was feasible for detecting abnormal contents of hypochlorous in a biological system and accomplished the goal of maintaining normal human activities. At last, the fluorescence imaging showed that MN-BODIPY was capable of localizing lysosomes in living cells.
研究领域:
分析化学
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生物化学
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细胞生物学
·
材料科学
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产品关联
DOI
高影响因子
TALANTA
·
2017
·
IF 4.162
Yan Ji,Li-Jun Xia,Liu Chen,Xiao-Feng Guo,Hong Wang,Hui-Juan Zhang
Hydrogen sulfide (H 2 S) is a critical biological messenger in numerous physiological and pathophysiological processes. Small-molecule fluorescent probes combined with fluorescent microscopy have been developed for the sensitive detection of H 2 S. Here, we designed and synthesized a long-wavelength BODIPY-based probe (TMSDNPOB) based on the thiolysis of dinitrophenyl ether according to photo-induced electron transfer theory and the results of computational calculation. 4,4-Difluoro-8-phenyl-1,5,7-trimethyl-3-(4-(2,4-dinitrophenoxy)styryl- 4-bora-3a,4a-diaza-s-indacene (TMSDNPOB) is nearly non-fluorescent, but the reaction product (TMSHOB) emits strong red fluorescence at 592 nm when excited at 574 nm. The long wavelengths of the designed probe indicate low background interference from biological matrix and little photo damage on fluorescence imaging of H 2 S. With the advantages of the turn-on probe for H 2 S including high sensitivity, high selectivity , good biocompatibility and low toxicity, the probe has been used for imaging H 2 S in living cells and liver tissues.
研究领域:
化学
·
生物化学
·
分子生物学
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荧光成像
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生物信号
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Sheng-Song Li,Yong-Chao Zheng,Xiao-Ming Zhu,Hong-Bo Wang,Long-Hui Liang,Xu-Zhe Wang,Ling Yuan,Fa-Heng Zhang,He Zheng,Chong-Lin Zhao
A fluorescent BODIPY probe (SO-BOD) conjugated with a 2-salicylaldoxime group via an ethynyl spacer was designed and synthesized for highly sensitive and selective detection of DCNP (a simulant of Tabun). The probe exhibited significant fluorescent quenching in the presence of Et 3 N, and 30-fold fluorescence enhancement was achieved in the presence of DCNP. Integration of experimental and computational methods well illustrated that these fluorescence variations were attributed to the intramolecular photo-induced electron transfer (PET) process. Except for the low limit of detection (34 nM) to DCNP, SO-BOD also showed a rapid response (t 1/2 = 175 s) and high selectivity. Furthermore, naked-eye detection of DCNP was carried out in both the liquid and vapor phases by test strips immobilized with the probe.
研究领域:
化学
·
分析化学
·
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
DYES AND PIGMENTS
·
2018
·
IF 3.767
Zhuo Ye,Chao Xiong,Jian Pan,Dongdong Su,Lintao Zeng
A series of highly photostable, lysosome-targeted BODIPYs with tunable fluorescence from green to the near-infrared region was developed. The probes were found to show an accurate lysosome-targeting ability with high selectivity and high photostability under continuous excitation irradiation. The results show that these lysosome-targeted BODIPYs offer a simple and convenient tool for fluorescence imaging of lysosomes, which will be valuable for understanding the activity and function of lysosomes in related cells.
研究领域:
细胞生物学
·
生物化学
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DOI
高影响因子
SENSORS
·
2025
·Vol. 25
·期 20
·6332
·
IF 3.5
Junyu Qu,Yixuan Liu,Wenqiang Fang,Huitao Liu,Zhenbo Liu
Sulfur dioxide (SO2) is commonly employed as an antioxidant and preservative in food processing, but excessive intake of SO2can pose significant health risks. Therefore, accurate detection of sulfite content in food is crucial for ensuring food quality and safety. A novel fluorescent probe, BODIPY-Y, composed of a BODIPY derivative and an ethyl cyanoacetate group linked by a carbon–carbon double bond, was synthesized for detecting sulfur dioxide derivatives. When the BODIPY-Y probe interacts with SO32−, the probe exhibits enhanced fluorescence at 514 nm. Spectrometric experiments show that the probe exhibits high sensitivity (LOD: 0.263 μmol/L), a fast response time (50 s) and excellent selectivity for SO32−. Mechanistic studies confirm that the BODIPY-Y probe operates via an intramolecular charge transfer (ICT) mechanism. The carbon–carbon double bond in BODIPY-Y undergoes nucleophilic addition with SO32−, blocking the ICT process and resulting in a blue shift in the fluorescence spectrum. In addition, the probe was applied to quantify SO32−levels in real food samples. The measured concentrations of SO2in the white sugar and red wine were 15.93 μmol/L and 7.30 μmol/L, respectively, with recovery rates of 77.9–98.1%. This work presents a prospective chemical tool for monitoring sulfur dioxide derivatives in food products.
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产品关联
DOI
PubMed
高影响因子
Journal of Biomaterials and Tissue Engineering
·
2022
·Vol. 12
·期 8
·1537-1541
·
IF 0.144
Wang Jianan,Tian Chao,Cao Zhengguo
Here in this study, we proposed a polystyrene maleic anhydride (PSMA) stabilized Bodipy nanoparticles (PB NPs) in a one-pot approach for the photodynamic therapy (PDT) of prostate cancer. The nanoparticle formed by precipitation method was then employed to treat PC-3 cells and PC-3
tumor bearing nude mice model. It was shown that this platform showed promising anticancer performance than free bodipy with reduced side effects.
研究领域:
纳米医学
·
光动力疗法
·
癌症研究
·
药物递送
·
材料科学
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DOI
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2025
·
IF 4.7
Bei Jiang,Jintao Jiang,Xinrui He,Yilei Zhu,Chao Guo
Accurate quantification of total antioxidant capacity (TAC) is crucial for evaluating the overall antioxidant activity in biological systems and food products. This is of growing importance in diverse applications such as health risk assessment and nutritional recommendations. Here, we introduce a novel Förster resonance energy transfer (FRET)-based sensor for the determination of TAC. The sensor utilizes an iodine-mediated FRET mechanism, employing BODIPY as the donor and Nile Red as the acceptor, both encapsulated within polystyrene (PS) microspheres. In the presence of iodine, its lipophilicity facilitates adsorption into the PS microspheres, resulting in fluorescence quenching due to the heavy-atom effect. This quenching results in an increased distance between the unquenched donors and acceptors, thereby decreasing FRET efficiency. Conversely, the presence of antioxidants reduces iodine concentration, which increases FRET efficiency. This dynamic allows for the development of a ratiometric fluorescence method for TAC determination. Critically, the sensor demonstrates exceptional photostability and resilience to variations in pH and ionic strength, contributing to its analytical robustness. The proposed method exhibits a linear response to ascorbic acid concentrations ranging from 0 to 50 μM, exhibiting good sensitivity (LOD = 2.5 μM) and selectivity. Additionally, the method has been successfully applied to evaluate TAC in ascorbic acid chewable tablets and lemon juice, demonstrating its potential as a robust and efficient tool for TAC determination.
研究领域:
分析化学
·
生物医学工程
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产品关联
DOI
高影响因子
TEXTILE RESEARCH JOURNAL
·
2016
·
IF 1.299
Ying-di Mu,De-chao Guo,Yan-jun Hou,Shu-hong Wang
In this work, two kinds of 4, 4-difluoro-4-bora-3a, 4a-diaza-s-indacene (BODIPY) composite nanofibers with excellent emitting properties are reported. These contain 5,5-difluoro-1,3,7,9-tetramethyl-10-phenyl-5H-dipyrrolo[1,2-c:2′,1′-f][1,3,2]diazaborinin-4-ium-5-uide (DBDP) and 10-(4-(diphenylamino)phenyl)-5,5-difluoro-1,3,7,9-tetramethyl-5H-dipyrrolo[1,2-c:2′,1′-f][1,3,2]diazaborinin-4-ium-5-uide (TBDP). DBDP and TBDP, which were obtained using a one-pot process, were respectively incorporated into the poly(methylmethacrylate) (PMMA) matrix with different concentrations and thus formed two series of BODIPY luminescent nanofibers via electrospinning technology. The composite nanofibers were further characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA) and luminescence. The SEM images show that the composite nanofibers have a smooth and uniform surface with a diameter about 0.4 µm. FT-IR spectra analysis indicates that the BODIPY compounds were successfully doped into the PMMA matrices. The TGA results show that decomposition temperature of BODIPY nanofibers was increased to about 113℃ and 88℃ for TBDP and DBDP, respectively, compared with that of BODIPY compounds. Furthermore, the BODIPY nanofibers also exhibit excellent optical fluorescence properties compared with the BODIPY compounds in dichloromethane (BODIPY/DCM) or in a solid state.
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Chunqing Li,Qi Sun,Qiang Zhao,Xinjian Cheng
It’s of vital importance to detect heavy metals in environment and living cells. In this work, four near-infrared regions boron dipyrromethene (BODIPY) probes (QBPH, PBPH, QBP and PBP) are constructed based on two BODIPY precursors (QB, PB) for sensing of Fe 3+ . As expected, these four probes exhibit obvious colorimetric and ratiometric response to Fe 3+ . In addition, QBP and PBP display highly sensitive and selective performance for detection of Fe 3+ . More importantly, QBP and PBP are successfully applied to near infrared imaging and detection of Fe 3+ in living A549 cells; it indicates that these novel designed probes could be a useful tool for the studies of Fe 3+ in living cells.
研究领域:
生物化学
·
分析化学
·
细胞生物学
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DOI
高影响因子
MATERIALS & DESIGN
·
2016
·
IF 3.997
Xinjian Cheng,Yun Yu,Yiru Jia,Lian Duan
Three small molecular boron dipyrromethene (BODIPY) derivatives (B3a, B3b and B3c) were well-designed and synthesized at first, and then were incorporated into polyurethane to obtain macromolecular fluorescent sensors. These small molecular BODIPY derivatives exhibited high sensitivity and selectivity to Hg 2 + , Cr 3 + and Fe 3 + ions. The colors of these chemicals changed rapidly upon the addition of Hg 2 + , Cr 3 + and Fe 3 + ions. In addition, their fluorescence peaks shifted and was enhanced as well. B3a, B3b and B3c still remained the sensing properties when being introduced to polyurethane (PU). The functionalized PU films not only showed color change and fluorescence shift for detecting Hg 2 + , Cr 3 + and Fe 3 + ions as their small molecular counterparts, but also triggered a new emission peak in 500–600 nm. Besides, the functionalized PU film (PU-B3a as a sample) could absorb Hg 2 + ions more than 84 mg/g in unit time and volume. It suggested that as long as the structure of the small molecular sensor was remained, the fluorescent ability remained even it is introduced to the macromolecular chains. In addition, Glass transition temperature (T g ) of the PU films changed when B3a, B3b and B3c were introduced.
研究领域:
材料科学
·
环境科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Jian Xu,Hui Yu,Yue Hu,Mingzhong Chen,Shijun Shao
A novel gold nanoparticle (AuNP)-based sensor for detecting thiols in aqueous solution has been developed. Due to the weak N···Au interactions, meso -(4-pyridinyl)-substituted BODIPY (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) dyes were coordinated to AuNP surfaces, which effectively quenched the fluorescence of organic/inorganic hybrid systems. The fluorescent quenching mechanism was mainly ascribed to the highly efficient fluorescent resonance energy transfer (FRET) and the inner filter effect. In the presence of thiols, meso -(4-pyridinyl)-substituted BODIPY chromophore were displaced and released from the AuNP surfaces and thus restored the fluorescence of BODIPY chromophore. The modulation of the fluorescence quenching efficiency of BODIPY–AuNPs in the presence of thiols can achieve a large turn-on fluorescence enhancement (40-fold) in aqueous solution. The new AuNP-based fluorescence sensor displayed desired properties such as high specificity, relatively low detection limit (30 nM for Cys), appreciable water solubility and rapid response time (within 2 min for Cys/Hcy). Moreover, the sensor has been successfully applied for monitoring and imaging of intracellular thiols within living HeLa cells .
研究领域:
生物化学
·
纳米生物学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2020
·
IF 3.306
Muhammad Amir Masood,Yuncong Chen,Shankun Yao,Shumeng Li,Weijiang He,Zijian Guo
A new BODIPY based Pd (II) complex, Pd-BDPA has been developed as a dual fluorometric and colorimetric probe for the detection of sulfide anion. The sensing ability is based on the interaction affinity of the Pd (II) towards the sulfide anion which is highly selective and immediate with a detection limit of 240 nM. The interaction between Pd (II) and sulfide is not only highly selective over the other common anions but also not interfered by their coexisting. This selective interaction between them leads to the regeneration of free ligand resulting in the dramatic changes in both fluorescence and absorption spectra. The fluorescent changes lead to quenching the emission spectra which is linear with the concentration of sulfide in the range of 1-10 μM. In addition to fluorescence quenching, the interaction also resulted in the significant color switching from purple to blue which was easily distinguished by the naked eye. Another important advantage is that the Pd-BDPA coated paper-based strips show the sulfide selectivity and sensitivity by the color changes as is in the solution form, revealing its practical utility more conveniently. In addition, the Pd-BDPA probe can also successfully be utilized for the determination of sulfide in real water samples with satisfactory recovery results indicating its feasibility for environmental samples.
研究领域:
分析化学
·
环境科学
·
生物化学
✓ 已验证
DOI
高影响因子
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