Communications Biology
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2025
·Vol. 8
·期 1
·1-13
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IF 5.1
Hu Bo,Deng Yuping,Lu Tao,Ren Miaomiao,Liu Kuitun,Rao Cong,Guo Hailiang,Su Jianya
Synthetic insecticides have been widely used for the prevention and control of disease vectors and agricultural pests. However, frequent uses of insecticides have resulted in the development of insecticide resistance in these insect pests. The resistance adversely affects the efficacy of insecticides, and seriously reduces the lifespan of insecticides. Therefore, resistance management requires new strategies to suppress insecticide resistance. Here, we confirm that CncC/Maf are the key regulators of various detoxification genes involved in insecticide resistance in Spodoptera exigua . Then, we develop a cell screening platform to identify the natural compound inhibitors of CncC/Maf and determine that sofalcone can act as a CncC/Maf inhibitor in vitro and in vivo. Bioassay results showed that sofalcone significantly enhanced the toxicity (more than 3-fold) of chlorpyrifos and lambda-cyhalothrin against S. exigua larvae. Finally, we demonstrate that sofalcone can greatly improve the susceptibility of S. exigua larvae to insecticides by inhibiting the activity of the ROS/CncC-dependent detoxifying enzymes and downregulating the expression levels of detoxification genes. CncC/Maf inhibitors can be used as broad-spectrum synergists to overcome insecticide resistance in pest populations. Altogether, our results demonstrate that reduced expression of detoxification genes resulting from suppression of transcriptional regulation of these genes contributes to controlling insecticide resistance, which provides a very novel and high-efficiency green resistance management strategy.
研究领域:
昆虫学
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分子生物学
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害虫管理
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Xiaojun Wang,Binbin Liu,Zhili Liu,Jing Li,Runhua Lu,Haixiang Gao,Canping Pan,Wenfeng Zhou
The main goal of this study is to create a CS-CMC-SF aerogel consisting of chitosan sodium carboxymethylcellulose and silk fibroin. The aerogel is designed to remove types of dyes from water while also being environmentally friendly. This innovative adsorbent has been optimized for extracting both cationic and anionic dyes from solutions. It incorporates chitosan sodium carboxymethylcellulose and silk filament fibers to enhance its strength . Experimental data illustrates that the CS-CMC-SF aerogel possesses remarkable adsorption capabilities - 5461.77 mg/g for Congo Red (CR), 2392.83 mg/g for Malachite Green (MG), and 1262.20 mg/g for Crystal Violet (CV). A kinetic study aligns with the pseudo-second-order kinetic model suggesting predominant chemisorption phenomena occur during adsorption process. Isotherm analysis further identifies multilayered adsorption occurring on irregularly shaped surfaces of the aerogel while thermodynamic assessments validate exothermic and spontaneous characteristics inherent in its absorption mechanism. Several analytical methods such as SEM, FT-IR, XRD, and XPS were employed to examine physicochemical attributes tied to this unique material design conceptually; identifying mechanisms including pore filling, π-π interactions, ion exchange activity, electrostatic connections along with hydrogen bonding inducing overall superior performance output. Furthermore substantial soil biodegradability alongside compostable features associated with our proposed CS-CMC-SF aerogels established it's potential suitability within applications demanding sustainable options thereby validating its underlying ecological credibility.
研究领域:
环境科学
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材料科学
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化学工程
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水处理
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PEST MANAGEMENT SCIENCE
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2026
·
IF 4.2
Wanwan Wang,WenTao Tang,Haiyun Rui,Qing Gao,Yichen Feng,Long Xu,Yonggen Lou
BACKGROUND Chemical elicitors offer a promising strategy for sustainable pest control by activating plant defense responses. The elicitor 4-fluorophenoxyacetic acid (4-FPA) induces resistance in rice against the white-backed planthopper (WBPH; Sogatella furcifera ) by triggering deposition of phenolic polymers that form a physical feeding barrier. However, whether piercing-sucking insects possess counter-defense strategies against such elicitor-induced resistance remains unknown. RESULTS We found that infestation by gravid WBPH females (WBPH-GF) systemically suppressed 4-FPA-induced phenolic polymer deposition without degrading pre-formed deposits, thereby restoring nymph survival. Metabolomic analysis revealed that WBPH-GF reprogrammed 4-FPA metabolism from side-chain cleavage to 4-fluorophenol toward conjugation with l -aspartic acid and l -glutamic acid, with the 4-FPA-aspartate conjugate increasing approximately 56-fold. This shift correlated with up-regulation of the amide synthetase gene OsGH3.8 and maintenance of aspartate and glutamate levels. Crucially, this process requires jasmonate (JA) signaling, as demonstrated by JA-deficient mutants and exogenous methyl jasmonate application. CONCLUSION Our findings reveal that WBPH-GF overcomes 4-FPA-induced resistance by hijacking the host JA pathway to amplify a latent metabolic shunt that diverts the elicitor into inert amino acid conjugates, thereby depleting the substrate for defensive polymer formation. This work uncovers a previously uncharacterized counter-defense strategy wherein a herbivore co-opts plant hormone signaling to metabolically disarm an exogenous elicitor, providing important insights for designing next-generation plant chemical elicitors with metabolic stability. © 2026 Society of Chemical Industry.
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RSC Advances
·
2024
·Vol. 14
·期 7
·4315-4323
·
IF 3.9
Chun Wang,Yu Shan,Yuli Shen,Weng Fu,Jing Li,David Blersch,Wei Wu,Suan Shi,Lujia Han
During the utilization of lignocellulosic biomass such as corn stover, many by-products are produced in the pretreatment process that can severely inhibit the activity of microbes in the fermentation step. To achieve efficient biomass conversion, detoxification is usually required before microbial fermentation. In this study, the prehydrolysate from dilute acid pretreatment of corn stover was used as a lactic acid fermentation substrate. Biochars made from corn stover (CSB), cow manure (CMB), and a mixture of corn stover and cow manure (MB) were applied for the detoxification of the prehydrolysate. All three types of biochar had a porous structure with a specific surface area ranging from 4.08 m2 g−1 (CMB) to 7.03 m2 g−1 (MB). After detoxification, both the numbers of inhibitors and their concentrations in the prehydrolysate decreased, indicating that the biochars prepared in this study were effective in inhibitor removal. The concentration of lactic acid obtained from the prehydrolysate without detoxification was only 12.43 g L−1 after fermentation for 96 h with a productivity of 0.13 g (L h)−1. Although the specific area of CMB was the lowest among the three biochars, the CMB-treated prehydrolysate resulted in the highest lactic acid concentration of 39.25 g L−1 at 96 h with a productivity of 0.41 g (L h)−1. The lactic acid bacteria in the CMB-treated prehydrolysate grew faster than the other two biochars, reaching an OD value of 8.12 at 48 h. The results showed promise for the use of agricultural wastes to make biochar to increase the yield of lactic acid fermentation through the detoxification process.
研究领域:
生物技术
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环境科学
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PEST MANAGEMENT SCIENCE
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2020
·Vol. 76
·期 12
·4036-4045
·
IF 3.75
Yifan Li,Hong Sun,Zhen Tian,Xinxin Su,Yue Li,Xuan Ye,Yifei Zhou,Shengli Zheng,Jiyuan Liu,Yalin Zhang
BACKGROUND Insect glutathione S-transferases (GSTs) play a crucial role in insecticide detoxification. However, there remains a distinct lack of information regarding the role of GSTs in the detoxification of Tolfenpyrad (TFP) in insects. RESULTS Real-time quantitative PCR showed significant upregulation of PxGSTs after exposure to TFP for 6 h. An in vitro inhibition assay showed that TFP could inhibit PxGSTδ, PxGSTε and PxGSTσ, and the most pronounced inhibitory effect was on PxGSTσ. Metabolism assays displayed that PxGSTσ was superior to other test PxGSTs in metabolizing TFP. The molecular docking of TFP and PxGSTσ revealed that the H-bond provided by the sidechains of Tyr107 and Tyr162 were key to the detoxification of TFP by PxGSTσ. Further tests using mutant PxGSTσ proteins at the sites of Tyr107 (PxGSTσY107A) and Tyr162 (PxGSTσY162A) corroborated that the individual replacement of Tyr107 and Tyr162 could greatly weaken the binding and metabolic abilities to TFP. CONCLUSION Metabolic interactions between the Plutella xylostella (L.) GSTs (PxGSTs) and TFP were deciphered. This study illustrates the molecular metabolism mechanism of PxGSTσ towards TFP and provides theoretical underpinnings for the design and optimization of novel TFP-like insecticides. © 2020 Society of Chemical Industry
研究领域:
昆虫学
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分子生物学
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农药科学
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SEPARATION AND PURIFICATION TECHNOLOGY
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2025
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IF 9
Tao Zhang,Xiaojuan Wang,Jie Gu,James M. Tiedje
Selective P O bond cleavage in organophosphorus pollutants under acidic conditions represents a key challenge in phosphatase-mimicking nanozyme design. Inspired by natural phosphatases, we designed and synthesized Zr x Co y -ZIF nanozymes featuring a Zr-N 4 coordination framework, achieving alkaline-free detoxification (t 1 / 2 =0.3 min, detoxification efficiency > 99 %). Theoretical analyses reveal that the oxygen-bridged proton shuttle activates interfacial water to supply protons for P O bond cleavage, while the Zr-Co electron transfer system reduces polarization energy barriers, enabling dual-site synergistic catalysis. Additionally, its magnetism facilitates material recovery, ensuring environmental friendliness. Notably, Zr x Co y -ZIF efficiently cleaves phosphodiester bonds and disrupts biofilms, removing > 90 % both Gram-positive and Gram-negative bacteria, with excellent antibacterial effects in real samples (lettuce, chicken, milk; removal rate > 85 %), demonstrating potential as a novel high-efficiency antimicrobial agent.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2022
·
IF 9.965
Min Wang,Guanghua Lu,Runren Jiang,Tianjian Dang,Jianchao Liu
In view of the increasing threat of overuse of broad-spectrum antibiotics to water environment, here, a series of small molecular intercalated bismuth oxychloride (SBC-X) composite photocatalysts were successfully constructed by a simple stirring synthesis at room temperature. Among them, SBC-0.5 showed excellent photocatalytic performance against the three target broad-spectrum antibiotics in visible light, which was 3.06 times, 5.93 times and 11.64 times higher than that of monomer for degrading tetracycline, norfloxacin and ciprofloxacin, respectively. Through analysis, it was found that the excellent photocatalytic degradation performance of SBC-0.5 was mainly attributed to the greatly improved specific surface area, which increased to 14 times of monomer, providing a large number of reaction sites for the subsequent photocatalytic degradation. Besides, intercalated molecules as charge transfer bridges between nanosheets greatly accelerated the efficiency of photogenerated charge transfer between layers. Free radical trapping experiments and electron spin resonance indicated that superoxide anion radicals played a major role in the photocatalytic degradation, followed by singlet oxygen. Furthermore, nine potential degradation intermediates were identified, and the toxicity was greatly reduced confirmed by ECOSAR software prediction and soybean seed germination and seeding growth experiment. Our work will provide useful information for the purification of wastewater containing antibiotics.
研究领域:
环境科学
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材料科学
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微生物学
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DOI
高影响因子
RENEWABLE ENERGY
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2025
·
IF 9.1
Jian Han,Sang-Hyun Pyo,Yong Xu
Resin adsorption is an effective technology for the fermentable sugar slurry detoxification in lignocellulosic biorefinery, but with the great limitation of the loss of resin capacity from inhibitor contamination and the extensive use of acid and base solutions for their regeneration. In this study, we developed a green and practical method with ultrasound assisting the depletion of poisoned resins in a reusable low concentration sodium hydroxide solution. The process, optimized at 50 % (325 W) ultrasonic power for 30 min, recovered 70 % resin adsorption capacity, balancing regeneration efficiency with minimal physical damage. SEM and FT-IR analyses were used to investigate the surface and chemical changes, while regeneration kinetics were modeled using the Boyd diffusion model. The regenerated resins were evaluated through multiple adsorption-desorption cycles and fermentation of detoxified acidic corncob hydrolysate (ACH) for xylonic acid production using Gluconobacter oxydans . During three rounds of regeneration, the productivity of xylonic acid was maintained between 7.05 g/L/h and 8.77 g/L/h with 100 % yield, which was improved 25 %–55 % over ACH without resin detoxification. This approach not only enhances the economic feasibility of lignocellulosic hydrolysate detoxification but also advances the biotechnological production of high-value chemicals from lignocellulosic biomass.
研究领域:
生物工程
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环境科学
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BIOSENSORS & BIOELECTRONICS
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2025
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IF 10.5
Xiao Liang,Zhuobing Zhang,Yilei Du,Ziliang Li,Nana Xu,Jian Gao,Huimin Qin,Liping Du,Song You,Jinshan Li,Weidong Liu,Yanhe Ma
Ochratoxins, toxic mycotoxins present in food and feed, pose a threat to human health due to their various toxic effects. Developing a safe, convenient, and rapid on-site detection tool for monitoring ochratoxins is essential for food and environmental safety, especially in developing countries. Although numerous effective ochratoxin sensing and detection systems are available, most do not combine detoxification with detection capabilities. In this work, an ochratoxin-detoxifying enzyme was cascaded with phenylalanine dehydrogenase to construct a colorimetric biosensor capable of simultaneously detecting and removing Ochratoxin A (OTA) and Ochratoxin B (OTB) contamination. Once the ochratoxins are detoxified into non-toxic OTα/OTβ and phenylalanine, 1-methoxy-5-methylphenazinium methyl sulfate (MPMS) functions as a signal reporter, converting the ochratoxin concentration into a colorimetric signal that gradually shifts from red to green. This allows for real-time visual observation with the naked eye. Additionally, OTA/OTB within a concentration range of 0–50 μg/mL can be quantitatively analyzed using a microplate reader or a smartphone. Under the optimized conditions, the colorimetric biosensor effectively eliminated and detected OTA/OTB at a spiked concentration of 5 μg/L in Chinese liquor samples within 1 h. Hence, the dual-enzyme cascade colorimetric biosensor has been demonstrated to be a safe, portable, rapid, and accurate tool for the detection and elimination of OTA/OTB in food.
研究领域:
生物传感技术
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食品安全
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环境安全
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酶催化
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分析化学
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PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2025
·
IF 4
Linlin Liu,Min Shi,Yanyan Wu,Jiali Hao,Junxiu Guo,Shanshan Li,Pingli Dai,Jing Gao
The widespread use of pesticides poses a significant threat to honeybee health by impacting their survival, behavior, immune function, and detoxification capacity. While phytochemicals such as resveratrol (RSV) have shown potential in mitigating oxidative stress and enhancing antioxidant defenses, their role in improving honeybee tolerance to pesticide exposure remains underexplored. In this study, we investigated the effects of RSV supplementation on honeybees exposed to three pesticides: dinotefuran (DIN), tebuconazole (TEB), and deltamethrin (DEL). The results showed that RSV supplementation significantly improved survival, feed intake, mobility, and gustatory sensitivity, indicating its protective effects against pesticide toxicity. Furthermore, RSV helped normalize impaired detoxification enzyme activities, including SOD, POD, catalase, and glutathione reductase, and reduced ROS levels and lipid peroxidation. Gene expression analysis revealed that RSV modulates Toll pathway-related genes like defensin and apidaecin , alleviating immune suppression caused by pesticides. Additionally, RSV influenced the insulin/insulin-like growth factor signaling (IIS) pathway by reducing ilp1 and inr1 expression, potentially mitigating metabolic stress. These findings demonstrate that protective effects of RSV may be linked to its ability to counter oxidative stress, restore mitochondrial function, and enhance energy metabolism. Furthermore, RSV is widely available, cost-effective, and easily incorporated into bee feed, making it feasible for large-scale application. This study highlights the protective role of RSV in pesticide detoxification in honeybees, offering new perspectives for honeybee health management and environmental toxicology research. By reducing the adverse effects of pesticides on honeybees, the application of RSV not only contributes to maintaining ecological balance but also supports sustainable agricultural practices. Future research should focus on optimizing its dosage, evaluating long-term effects, and investigating its impact on colony dynamics to facilitate its practical implementation in apiculture.
研究领域:
昆虫学
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环境毒理学
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分子生物学
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JOURNAL OF HAZARDOUS MATERIALS
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2026
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IF 11.3
Munire Abuduaini,Yuxuan Sang,Zhangzhang Xie,Yuechao Zhang,Oumei Wang,Liangyan Li,Fanghua Liu
Chloramphenicol (CAP), a chlorinated nitroaromatic antibiotic, posed significant ecological and human health risks due to its persistence and toxicity. Although microbial degradation represented a promising remediation strategy, its practical application was often constrained by slow degradation kinetics and incomplete biotransformation. In this study, rapid and efficient CAP degradation by the Gram-positive anaerobe Clostridium pasteurianum DSM 525 was demonstrated. The strain achieved 83.6% removal of 400 mg L -1 CAP within 8 hours, and the degradation kinetics followed a first-order model (R 2 ≥ 0.91). Metabolite analysis revealed a nitroreduction pathway, with CAP initially transformed into 4-aminophenyl allyl alcohol and subsequently converted into a dichlorinated aromatic amine (AMCl 2 ). Integrated transcriptomic analysis uncovered a coordinated cellular response underlying the high degradation efficiency. Genes involved in the pentose phosphate pathway were significantly up-regulated, indicating enhanced NADPH supply. In parallel, a nitroreductase gene (log 2 FC = 2.43) responsible for antibiotic reduction and an EamA family transporter potentially involved in CAP uptake (log 2 FC = 2.07) were markedly induced, together with the up-regulation of genes encoding F 0 F 1 -ATP synthase, which was likely involved in ATP-dependent proton translocation and maintenance of transmembrane electrochemical gradients to support substrate transport. Concurrently, global stress-response mechanisms were activated to alleviate CAP-induced cellular stress. Collectively, this study elucidated the metabolic coordination underlying efficient CAP biodegradation and highlighted the potential of anaerobic Gram-positive bacteria for the bioremediation of nitroaromatic antibiotics.
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Small
·
2026
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IF 11.8
Runren Jiang,Hanqi Yue,Yuyue Liu,Yilin Deng
Developing efficient water purification technologies without external chemical inputs is critical for treating emerging contaminants. Herein, we report a Cu 2 WS 4 -CoPc composite piezocatalyst that enables efficient in situ H 2 O 2 production and pollutant degradation via enhanced oxygen activation. The introduction of CoPc induces interfacial electron transfer from Cu 2 WS 4 to CoPc and promotes O 2 activation through d-p orbital hybridization between Co and O 2 , as confirmed by density functional theory calculations showing a decrease in O 2 adsorption energy from +1.2 to −0.3 eV. Consequently, the composite exhibits significantly enhanced piezoelectric response, with increased piezoelectric constant and effective longitudinal piezoelectric coefficient. The optimized Cu 2 WS 4 -CoPc generates H 2 O 2 via a two-electron oxygen reduction pathway, with superoxide radicals (·O 2 − ) as the key intermediate, achieving a yield of 183.4 µM within 30 min, which far exceeds that of pristine Cu 2 WS 4 . Moreover, the catalyst degrades ciprofloxacin with a rate constant of 0.059 min −1 , approximately 4.5 times higher than pure Cu 2 WS 4 , and zebrafish toxicity assays confirm effective detoxification of degradation intermediates. This study provides a new strategy for enhancing piezocatalytic H 2 O 2 synthesis through strengthened oxygen activation and lays a theoretical foundation for designing efficient, environmentally benign water treatment technologies.
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