COLLOIDS AND SURFACES B-BIOINTERFACES
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2025
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IF 5.6
Jingyi Qu,Xinyue Yang,Zelin Sang,Yijia Zhang,Xinliang Zhu,Yang yang Qu,Zhenhua Chen,Cheng Li,Lei Li
Hyperglycemia usually causes microvascular damage, which leads to chronic wound healing and is highly susceptible to bacterial infections. Overdependence on antibiotics accelerates the evolution of drug-resistant strains, making the treatment of diabetes related infected wounds more complex and difficult. Therefore, it is urgent to develop new antibacterial materials and therapeutic regimens, especially for diabetic wound infected with drug resistant bacteria. Herein, we propose a strategy based on black phosphorus nano-snowflakes (BPSFs) enhanced hydrogel (BPSFs@H), in which the BPSFs function as photothermal-responsive nanozymes, dedicated to the repair of diabetic infected wounds. The designed BPSFs are near-infrared (NIR) triggered photothermal responsive, and are effective in inhibiting methicillin-resistant Staphylococcus aureus (MRSA) by generating reactive oxygen species (ROS). BPSFs@H hydrogel exhibited remarkable efficacy in promoting cell migration and neovascularization. Moreover, it demonstrated the capacity to modulate the secretion of bioactive components by macrophages, driving them toward an anti-inflammatory phenotype. Animal studies revealed that BPSFs@H hydrogel substantially promoted the regeneration of wound epithelial tissue and enhanced the healing efficacy of infected wounds. Through mechanisms including augmented collagen deposition, improved local inflammatory response, and accelerated angiogenesis, this hydrogel significantly enhances the wound repair process by promoting re-epithelialization.
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高影响因子
ACS Applied Materials & Interfaces
·
2022
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IF 10.383
Shanshan Qi,Ruifeng Luo,Xiaoqin Han,Wenbiao Nie,Naijing Ye,Chaomei Fu,Fei Gao
Rhein (RH), a natural anthraquinone compound, is considered an effective treatment candidate for ulcerative colitis (UC), whose multiple biological activities contribute to UC, including anti-inflammation, antioxidation, intestinal barrier repair, and microflora regulation. However, the application of RH is severely limited by its low water solubility, low bioavailability, and poor colonic targeting. Although some nanoparticles have been developed for the oral delivery of RH, most of them mainly highlighted only one effect of some drug delivery strategies but the above multiple biological activities. Therefore, a multiple polysaccharide-based nanodelivery system, comprising chitosan (CS) and fucoidan (FU), with pH/reactive oxygen species (ROS) sensitivity and mucosal adhesion, was developed and first used to load RH as a comprehensive treatment for UC. Briefly, RH-F/C-NPs were prepared using the polyelectrolyte self-assembly method; the average size of RH-F/C-NPs was 233.1 ± 5.7 nm, and the encapsulation rate of RH was 93.67 ± 1.60%. And it could maintain gastric stability and release RH in the colon with the designed pH/ROS sensitivity contributed by the polysaccharide-based structures. Cellular uptake experiments showed that both NCM 460 cells and RAW 264.7 cells had a good uptake of RH-F/C-NPs. Importantly, the effects of RH were highlighted in in vivo experiments, the results of which showed that RH-F/C-NPs could significantly reduce DSS-induced inflammation by inhibiting the TLR4/NF-κB-mediated anti-inflammatory pathway, the Nrf2/HO-1-mediated antioxidant pathway, colonic mucosal barrier repair, and intestinal microflora regulation. In addition, pharmacokinetic studies have shown that F/C-NPs contribute to the increase in the plasma concentration and the accumulation of RH in the colon to some extent. In short, this study is the first to develop an oral multiple polysaccharide-based nanosystem with pH/ROS dual sensitivity to study the “one stone four birds” therapeutic effect of RH on UC.
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TOXICOLOGY LETTERS
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2017
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IF 3.858
Huaicheng Chen,Zongming Song,Shilong Ying,Xi Yang,Wei Wu,Qiufan Tan,Xin Ju,Wencan Wu,Xin Zhang,Jia Qu,Yi Wang
Retinal ischemia reperfusion (I/R) injury is common in many ophthalmic diseases. Recent studies have shown that toll-like receptor 4 (TLR4) is involved in ischemic retinal injury. Activation of TLRs requires specific accessory proteins such as myeloid differentiation protein 2 (MD2), which facilitate in ligand responsiveness. Therefore, inhibiting MD2 may be a novel approach to modulate TLR4 signaling and deleterious downstream effects in ischemic retinal injury. We used human Müller MIO-M1 cells treated with tert-butyl hydroperoxide (TBHP) to establish an in vitro I/R model of oxidative injury and tested the therapeutic effect of inhibiting MD2. Furthermore, we inhibited MD2 in a mouse model of retinal I/R injury and confirmed the results using MD2 knockout mice. Our studies show that pharmacological inhibition of MD2 prevented TBHP-induced reactive oxygen species (ROS) generation, inflammation and subsequent apoptosis in Müller cells. We also show that retinal I/R injury in mice induced functional deficits, increased ROS levels, inflammation and apoptosis. These pathological changes were not observed in MD2 knockout mice and attenuated when MD2 was inhibited in wildtype mice. In addition, we discovered that the mechanism of these therapeutic effects involved regulation of NADPH oxidase 4 (NOX4)-MD2-TLR4 complex formation. This study provides evidence that MD2 plays a key role in the pathogenesis of retinal I/R damage by participating in TLR4-NOX4 complex formation and elaboration of oxidative and inflammatory damage. Hence, inhibition of MD2 may reduce TLR-dependent damage during retinal I/R injury.
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APPLIED CATALYSIS B-ENVIRONMENTAL
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2020
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IF 16.683
Yizhang Wu,Dongxin Yang,Wei Xu,Renjie Song,Mengmeng Li,Yong Wang,Boye Zhou,Niandu Wu,Wei Zhong,Hong-ling Cai,Juan Tu,Dong Zhang,X.S. Wu
Graphitic Carbon Nitride (g-C 3 N 4 ) has extensive application prospect of photodynamic therapy (PDT), which can be attributed to the biocompatibility and photosensitivity. However, it suffers from the near-ultraviolet absorption edge and low reactive oxygen species (ROS) release rate. This work exhibits an atomic-thickness water-soluble g-C 3 N 4 with alkali Zn 2+ and K + modification (MCN), whose absorption edge is tuned from 460 nm for GCN to the entire visible-light region (663 nm), and bandgap is reduced to 1.94 eV. Both K + - bridging intercalation and the in-plane Zn-N significantly enhance the photocatalytic performance, as well as inhibit the overexpression of glutathione (GSH) significantly. The synergistic effect make that the release rate of ROS is about 45.16 % (7.95 % for pristine g-C 3 N 4 ), accomplishing a considerable inactivation efficiency of malignant melanoma A375. This work fundamentally releases the limitation of g-C 3 N 4 as a PDT photosensitizer, revealing its multifunctional promise to cancer therapy.
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JOURNAL OF HAZARDOUS MATERIALS
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2026
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IF 10.6
Jiajia Wu,Shijie Yang,Guanhong Lu,Xiao Wang,Jing Sun
Efficient photocatalytic abatement of volatile organic compounds (VOCs) critically relies on effective separation of photogenerated electron-hole pairs and the generation of reactive oxygen species (ROS). Constructing S-scheme heterojunctions is an effective strategy to promote charge separation while preserving strong redox potentials. Herein, WO 3 /MIL-100(Fe) composites featuring interfacial Fe-O-W bonds were synthesized via a one-pot hydrothermal method for photocatalytic degradation of gaseous toluene. The optimized 15%WO 3 /MIL-100(Fe) heterojunction exhibits a 1.3-fold enhancement in toluene removal efficiency (52.6%) and twice the CO 2 conversion rate relative to pristine MIL-100(Fe), along with enhanced degradation for o-xylene and styrene, demonstrating broad-spectrum applicability. The interfacial internal electric field (IEF) in WO 3 /MIL-100(Fe) promotes efficient charge carrier separation and accelerates electron transfer. The S‑scheme heterojunction directs the reductive electrons in the MIL-100(Fe) conduction band and oxidative holes in the WO 3 valence band to generate superoxide radicals (•O 2 ⁻) and hydroxyl radicals (•OH), respectively, enabling efficient ring opening and deep degradation. Moreover, the load of WO 3 enhances Lewis acidity and introduces additional adsorption sites, strengthening the interaction with toluene. This work highlights the rational design of MOF-based S-scheme heterojunctions for durable volatile organic compound abatement.
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JOURNAL OF CONTROLLED RELEASE
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2026
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IF 12.4
Dongjing Wang,Xiaonan Li,Kunpeng Wei,Suyan Wang,Wen Zuo,Ze Xu,Chen Li,Hui Wang,Xueyan Zhou,Yanzhuo Zhang
Acute kidney injury (AKI) is a prevalent clinical syndrome associated with high morbidity, mortality, and a heavy medical burden. Oxidative stress, inflammation, and ferroptosis are key drivers of AKI, yet current pharmacotherapies are limited by suboptimal efficacy and systemic side effects. An efficient and precise kidney-targeted delivery system that co-delivers complementary therapeutics could overcome these challenges. Herein, we report a platelet membrane–coated, reactive oxygen species (ROS)-responsive nanozyme (BAPCs@PM) for synergistic AKI therapy. Ultrasmall CeO 2 nanodots (∼3 nm) were in situ mineralized on the outer layer of polydopamine cores, and hydrophobic baicalein was loaded into the mesoporous interior, yielding a stable and well-dispersed multifunctional composite nanozyme. Platelet membrane cloaking conferred renal targeting, immune evasion, and prolonged circulation. BAPCs@PM enabled ROS-triggered release of CeO 2 nanodots and sustained baicalein delivery at injured sites, amplifying local antioxidant, anti-inflammatory, and anti-ferroptotic activity. In a cisplatin-induced AKI model, BAPCs@PM markedly suppressed kidney injury molecule-1 upregulation, inhibited cleaved caspase-3 activation and apoptosis, enhanced mitochondrial protection, modulated macrophage polarization, reduced proinflammatory cytokine production, regulated iron homeostasis, restored glutathione peroxidase 4 expression, attenuated ferroptosis and lipid peroxidation, and ultimately improved renal function. Owing to their ultrasmall size, the ceria nanodots achieved efficient renal accumulation and rapid clearance with minimal systemic toxicity. This study provides an innovative biomimetic and responsive therapeutic strategy for AKI by combining ultrasmall nanozymes, natural bioactive molecules, and biomimetic membrane engineering.
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Advanced Agrochem
·
2026
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IF 9.2
Cuiyun Zhou,Qinghong Zeng,Chang Tang,Dan Sun,Zixuan Li,Shuilan Zhang,Tao Zheng,Zhaoyang Zhang,Mengke Wang,Shun He,Wa Gao
Nanomaterials offer new opportunities for sustainable control of crop diseases caused by soil-borne pathogens. Here, cobalt-encapsulated nitrogen-doped carbon nanotubes (Co@N-CNTs) with tubular and bamboo-like morphologies were synthesized and evaluated against tomato bacterial wilt. Co@N-CNTs showed strong antibacterial activity against Ralstonia solanacearum in vitro , with the tubular morphology being more effective. Mechanistic analyses indicated that Co@N-CNTs induced reactive oxygen species-mediated oxidative damage in R. solanacearum , resulting in DNA damage, cytoplasmic leakage, and membrane disruption. In pot experiments, Co@N-CNTs reduced disease severity and delayed progression, with efficacy comparable to Zhongshengmycin. Transcriptomic and biochemical analyses showed tissue-specific regulation of plant defense, reducing infection-induced SA accumulation in stems while enhancing the expression of several defense-related genes in leaves, whereas JA contents were not significantly altered. Co@N-CNTs showed no detectable adverse effects on tomato growth and exhibited low acute toxicity to Italian honeybees. Collectively, these findings demonstrate that Co@N-CNTs can control soil-borne bacterial diseases and hold promise for sustainable agriculture.
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Bioactive Materials
·
2026
·
IF 23.6
Xueheng Sun,Shangyu Xie,Ya Fang,Deteng Zhang,Wenhao Wang,Zeyue Sun,Jiaju Lu,Xiaokun Yue,Weize Kong,Dehao Fu,Xiang Xu,Kerong Dai,Jinwu Wang,Jie Wei,Xin Sun
The impaired regeneration of aged individuals presents major challenges for repairing bone defects. Within the senescent microenvironment (SME), excessive reactive oxygen species (ROS), chronic inflammation, the accumulation of senescent cells and local infection form a self-reinforcing vicious cycle that hinders healing. Here, we developed a multimodal ROS logic-gated therapeutic platform by integrating magnesium- and manganese-doped bismuth oxide with oxygen vacancies nanoparticles (MMBOx) embedded in a rapamycin (Rapa)-loaded, 3D-printed hydrogel scaffold. In vitro study, this platform combines pH-responsive peroxidase, oxidase-like activity and photothermal-enhanced sonodynamic effects, enabling on-demand ROS generation for efficient antibacterial elimination. Simultaneously, MMBOx and the hydrogel scavenge excess ROS, while Rapa promotes cellular autophagy to remove damaged mitochondria, enhances ROS regulation, and delays stem cell senescence. Moreover, MMBOx enhances glutathione (GSH) synthesis and metabolism by modulating the Keap1-Nrf2 signaling pathway, thereby boosting the intracellular GSH pool and increasing ROS tolerance to support a more youthful cellular phenotype. In vivo studies confirm that this platform alleviates infection in the infected skin defect model. Furthermore, it attenuates SME-associated chronic inflammation and cellular senescence, and promotes bone regeneration in the aged rat calvarial defect model. These findings offer a promising strategy for addressing deteriorated SME and enhancing bone repair in the elderly.
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Molecular Plant
·
2026
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IF 32.7
Jingjing Xing,Kaiwen Li,Wenwen Duan,Zhikun Duan,Yu Zhang,Aiyu Guo,Suting Wang,Ning Ding,Yaling Zou,Xiaobin Wei,Xiaoliang Zhao,Junli Zhang,Yuwei Song,Huazhong Shi,Siyi Guo,Chun-Peng Song
Reactive oxygen species (ROS) function as essential signaling molecules regulating diverse processes in plants. However, the mechanisms by which plants accurately perceive and distinguish between physiological and stress-induced ROS signals to coordinate growth and stress responses remain largely unclear. Here, we demonstrate that Radical-induced Cell Death1 (RCD1) functions as a redox sensor that undergoes a ROS-triggered phase separation transition. Three cysteine residues within RCD1 contribute to the formation of phase-separated condensates, and the transition from condensate to non-condensate determines the spatiotemporal interactions of RCD1 with the leaf morphogenesis-related transcription factor ASYMMETRIC LEAVES1 (AS1) or the stress-responsive transcription factor zinc-finger protein ZAT12. Therefore, the phase separation transition of RCD1 differentiates between growth- and stress-related ROS signals to orchestrate downstream gene regulation. The dynamic formation of transcriptional condensates and ROS-induced phase separation transition allow plants to rapidly distinguish and respond to growth and stress cues, potentially representing a conserved mechanism in eukaryotes.
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INTERNATIONAL IMMUNOPHARMACOLOGY
·
2026
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IF 5.6
Yu Zhang,Yue Zhang,Qin Xiang,Xiangxiang Fu,Yongli Zhang,Liming Tan,Huaiying Tang,Siqi Li,Dong Gong,Xu Guo,Yonghong Zhang,Qibin Wang,Li Chen,Tao Zheng
Type 2 diabetes mellitus (T2DM) and prostate cancer are closely linked, affecting overlapping age groups of predominantly male populations. Flutamide (FLU) is metabolized in the liver to hydroxyflutamide (OHF), exerting anti-androgenic effects and is mainly used for prostate cancer treatment. During its use, occasional adverse reactions such as elevated blood glucose and worsening conditions in T2DM patients have been observed, but the cause remains unknown. This study aims to investigate the effects and mechanisms of FLU on hepatic insulin resistance under T2DM conditions. In vitro , a primary mouse hepatocyte model of insulin sensitivity impairment induced by high glucose (HG) was established to observe the effects of FLU/OHF on cellular lipid accumulation, reactive oxygen species (ROS) production, NACHT, LRR, and PYD domains-containing protein 3 (NLRP3) signaling, and insulin signaling. NLRP3 inhibitors, ROS scavengers, and hepatocytes from Nlrp3 -knockout mice were used to explore the mechanisms of FLU. In vivo , a high-fat diet (HFD)/streptozotocin (STZ) induced-T2DM mouse model was established to observe the effects of FLU gavage on glucose and lipid metabolism, insulin sensitivity, and histopathology. The results showed that FLU exposure exacerbated lipid accumulation, ROS production, over-activation of NLRP3 signaling, and impaired insulin signaling in HG-treated hepatocytes. Inhibition of NLRP3, scavenging of ROS, or knockout of Nlrp3 eliminated the above effects of FLU/OHF. FLU exacerbated glucose and lipid metabolism disorders in HFD/STZ induced-T2DM mice, increased hepatic insulin resistance, and activated the hepatic NLRP3 signaling pathway. The results of this study suggest that FLU treatment is associated with exacerbated hepatic insulin resistance in mice with T2DM, an effect that may be mediated through ROS overproduction and activation of NLRP3 signaling.
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高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2026
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IF 19.9
Zhen Jiang,Xingyu Zhu,Haiguang Zhu,Ze Li,Rui Li,Junlong Geng,Xun Yuan
Psoriasis is a chronic inflammatory skin disease driven by oxidative stress, necessitating advanced antioxidant nanozymes capable of broad-spectrum reactive oxygen species (ROS) elimination. Here, we address this challenge through the rational design of a ternary synergistic nanocluster, Ag 14–20 Au 5–11 @FeTP, engineered via “atomic engineering” of an optimal AgAu alloy core and “molecular engineering” via Fe‑porphyrin ligand grafting. This dual-level engineering creates a metal–ligand synergistic interface that confers excellent multi-enzyme mimetic activity for scavenging H 2 O 2 , •O 2 − , and •OH. Coupled with enhanced skin penetration, the nanozyme demonstrates both therapeutic and preventive efficacy in a psoriasis mouse model, rivaling the first-line clinical drug betamethasone/calcipotriol, without observable systemic toxicity. Transcriptomic profiling reveals that its potent effect originates from a dual-pathway immunomodulation: simultaneous suppression of the pro-inflammatory IL‑23/Th17/IL‑17 and NF‑κB axes and enhancement of the anti-inflammatory Treg/Th2 pathways. This work establishes a generalizable “precision synergy” paradigm for designing high-performance nanocluster-based therapeutics against oxidative stress-related inflammatory diseases.
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Advanced Science
·
2026
·
IF 14.1
Weiyu Zhang,Zhongwei Yang,Yunfan Zhang,Longwei Wang,Xiaoyu Zhang,Jiahui Mao,Yizhi Dai,Yifan Yuan,Mingge Wang,Xin Yang,Xin Yu,Jing Liu,Chunying Chen
Periodontitis, a chronic inflammatory disease initiated and sustained by plaque microorganisms and host immune response, remains an intractable oral disease and a leading cause of tooth loss worldwide. Traditional mechanical debridement and adjunctive antibiotic or antiseptic therapy often shows limited efficacy due to the complex anatomical structure, concerns regarding antimicrobial resistance, and poor penetration and retention within the subgingival infection niche. To overcome this limitation, we designed a Mo-N coordinated nanozyme exhibiting synergistic mimetic activities of multiple enzymes, including peroxidase (POD)-like, oxidase (OXD)-like, and catalase (CAT)-like activity. Benefiting from Mo-N coordination and multi-enzyme mimetic behavior, Mo 5 N 6 nanozymes dynamically modulate local oxidative reactions within the gingival sulcus, thereby effectively damaging pathogenic bacteria while avoiding excessive oxidative stress. The nanozymes efficiently suppress anaerobic Gram-negative periodontal pathogens sensitive to elevated reactive oxygen species (ROS), facilitating efficient attenuation of pathogenic stimuli. This strategy not only enhances the periodontal microenvironment but also facilitates the restoration of commensal microbiota and regeneration of periodontal tissues, highlighting the therapeutic potential of Mo 5 N 6 nanozymes in periodontitis treatment.
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