查找使用阿拉丁产品的科研文献

A Magnetic Colorimetric Aptasensor Based on Fe3O4@CQDs Nanocomposites for Ultrasensitive Detection of Glypican-3

Lingling Fan,Cunhong Cen,Yuru Wu,Xiaohua Deng,Yuxuan Shi,Yuxing Huang,Xiaohong Tan,Shidong Li,Guiyin Li

Hepatocellular carcinoma (HCC) remains one of the most lethal malignancies worldwide, underscoring the urgent demand for early diagnostic strategies to improve patient survival rates. Glypican-3 (GPC3), a highly specific biomarker of HCC, has emerged as a promising target for early detection. Herein, we developed an innovative magnetic colorimetric aptasensor using ferrosoferric oxide@carbon quantum dots (Fe₃O₄@CQDs) nanocomposites. The Fe₃O₄@CQDs nanocomposites combine the magnetic properties of Fe₃O₄ with the unique optical characteristics of CQDs, exhibiting intrinsic peroxidase‑like activity. The animo-modified GPC3 aptamer (GPC3_Apt) was covalently conjugated onto the Fe₃O₄@CQDs nanocomposites surface via EDC/NHS chemistry to form the recognition probe (Fe₃O₄@CQDs-GPC3_Apt). Upon specific binding between the aptamer and GPC3, the resulting complexes (Fe₃O₄@CQDs-GPC3_Apt-GPC3) modulate the catalytic oxidation of o-phenylenediamine (OPD) by H₂O₂, generating a yellow oxOPD product whose absorbance intensity correlated with GPC3 concentration. The proposed colorimetric aptasensor demonstrated a detection limit of 3.16 ng/mL and a linear range of 10–200 ng/mL, with high specificity against interfering proteins. It was successfully validated in human serum samples, yielding recoveries between 99.27% and 109.33%. Owing to its operational simplicity and cost‑effectiveness, this platform offers transformative potential for point-of-care HCC screening. Beyond advancing GPC3 detection, the work provides a flexible design paradigm that can be adapted for assays targeting other disease biomarkers.

Na3MnZr(PO4)3 fast ionic conductor modulates zinc flux to achieve homogeneous Zn plating/stripping

Mingjun Wu,Yiqing Ouyang,Mingjian Zhao,Yuyao Wu,Zhengying Wang,Qiang Ru

An artificial interphase of Na 3 MnZr(PO 4 ) 3 (NMZP) is engineered on the zinc metal surface as a fast ion conductor to efficiently modulate zinc flux and stabilize the anode. Fabricated via a sol-gel method, NMZP possesses a NASICON-type open 3D framework, high structural stability, robust corrosion resistance, and exceptional ionic conductivity, providing favorable pathways for Zn 2+ diffusion. The NMZP interphase modulates Zn 2+ flux through ionic confinement effects while promoting desolvation of [Zn(H 2 O) 6 ] 2+ species prior to deposition, thereby suppressing parasitic reactions. This dual mechanism ensures uniform zinc nucleation and deposition beneath the protective layer, effectively inhibiting dendritic growth and enhancing cycling stability. Benefiting from robust protective function of the NMZP layer, the NMZP@Zn symmetric cell achieves stable cycling beyond 650 hours at 3.0 mA cm -2 and 1.0 mAh cm -2 with minimal polarization. This study establishes an engineered interphase strategy for realizing long-lived, dendrite-free zinc-ion batteries.

A Janus Li1.5Al0.5Ge1.5(PO4)3 with high critical current density for high-voltage lithium batteries

Wenping Zha,Yadong Ruan,Zhaoyin Wen

The application of Li 1.5 Al 0.5 Ge 1.5 (PO 4 ) 3 (LAGP) is hindered by chemical instability with lithium (Li) metal and poor interfacial compatibility with high-voltage cathodes. Herein, an in-situ Janus LAGP is fabricated to solve the above two issues simultaneously. On the cathodic side, a succinonitrile-based interlayer with good oxidation stability is applied to facilitate ion transport at the interface and inside the cathode. On the anodic side, Fluoroethylene carbonate (FEC)-based polymer interlayer is chosen to ameliorate side reactions on LAGP/Li interface and inhibit the dendrite growth. Consequently, the Li symmetric cell with the Janus LAGP possesses a high critical current density of 5.4 mA cm −2 and operates stably for over 500 h at a high current density of 1.0 mA cm −2 . Moreover, the Janus LAGP exhibits excellent ionic conductivity (4.76 × 10 −4  S cm −1 ), wide electrochemical window (0–5.2 V vs. Li + /Li) and stable interfacial compatibility with high-voltage cathodes. The solid-state lithium battery (LiNi 0.8 Co 0.1 Mn 0.1 O 2 /Li) delivers a maximum discharge capacity of 178 mAh g −1 with a retention of 85.7% after 100 cycles at 0.5C. Notably, when the mass loading is increased to 10.0 mg cm −2 , the battery delivers a maximum discharge capacity of 134.1 mAh g −1 (1.52 mAh) at 0.5C, and the capacity remains 76.1% after 100 cycles.

Discovery of N-hydroxy-4-(1H-indol-3-yl)butanamide as a histone deacetylase inhibitor

Jiang Bian,Yepeng luan,Chunbo Wang,Lei Zhang

A highly selective chemosensor for Al3+ based on 2-oxo-quinoline-3-carbaldehyde Schiff-base

Ke Zhang,Zheng-yin Yang,Bao-dui Wang,Shao-Bo Sun,Ying-Dong Li,Tian-rong Li,Zeng-chen Liu,Jun-mei An

A new Schiff-base ligand ( 1 ) with good fluorescence response to Al 3+ , derived from 2-oxo-quinoline-3-carbaldehyde and nicotinic hydrazide, had been synthesized and investigated in this paper. Spectroscopic investigation revealed that the compound 1 exhibited a high selectivity and sensitivity toward Al(III) ions over other commonly coexisting metal ions in ethanol, and the detection limit of Al 3+ ions is at the parts per billion level. The mass spectra and Job’s plot confirmed the 1:1 stoichiometry between 1 and Al 3+ . Potential utilization of 1 as intracellular sensors of Al 3+ ions in human cancer (HeLa) cells was also examined by confocal fluorescence microscopy.

General chemical synthesis of cyanidin-3-O-glycosides: configuration determination and structure-activity relationship analysis

Zipeng Zheng,Jun Hu,Weijie Zhou,Hongqing Yuan,Jianxia Sun,Yongbin Xu,Weibin Bai

Anthocyanins are a class of water-soluble flavonoid pigments widely distributed in plants, characterized by their flavylium cation core. Glycosylation modifications exert significant effects on the biological activities of anthocyanins with identical aglycones. This study accomplished the first chemical syntheses of cyanidin-3-O-galactoside (C3Gal) and cyanidin-3-O-arabinoside (C3Ara), providing a general glycosylation strategy. The configurations of β-D-galactopyranoside and α-L-arabinopyranoside were determined by 2D NMR. Comparative analysis of cyanidin-3-O-glucoside (C3Glu), C3Gal, and C3Ara revealed that C3Glu was the most stable, whereas C3Ara and C3Gal exhibited superior antioxidant activity. C3Ara also showed the strongest inhibition against α-amylase (IC 50  = 98.16 μM), consistent with molecular docking predictions. These results reveal the key structure-activity: hexoses (glucose, galactose) have higher stability and reduced activity. Pentosyl arabinosides have higher biological activity. This work provides a reliable preparation method and research direction for anthocyanin glycosides.

Luminescence and energy transfer of the color-tunable phosphor Li6Gd(BO3)3:Tb3+/Bi3+, Eu3+

Peican Chen,Fuwang Mo,Anxiang Guan,Rongfang Wang,Guofang Wang,Siyu Xia,Liya Zhou

Bi 3+ /Tb 3+ , Eu 3+ co-doped Li 6 Gd(BO 3 ) 3 (LGBO) phosphors were synthesized via a traditional solid-state method. Phase purity was investigated using X-ray diffraction, absorption strength of the phosphors were investigated by UV–vis absorption spectra , and the photoluminescence properties of the phosphors were studied systematically. Results showed that the emission intensity of Bi 3+ , Eu 3+ co-doped LBOG was 2.76 times higher than that of Eu 3+ -doped LGBO irradiated at 275   nm, thereby implying the possibility of energy transfer from Bi 3+ to Eu 3+ . The excitation spectra of Tb 3+ , Eu 3+ co-doped LGBO phosphors are broader in comparison with single-doped phosphors and show tunable colors from green to yellow to orange-red when the ratio of Tb 3+ to Eu 3+ is adjusted These results demonstrate that LGBO:Tb 3+ , Eu 3+ phosphors have potential use in light-emitting diodes.

Preparation of corn starch-g-polystyrene copolymer in ionic liquid: 1-Ethyl-3-methylimidazolium acetate

Yongjun Men,Xiyan Du,Jianan Shen,Leli Wang,Zhengping Liu

The copolymer of starch grafted with polystyrene (starch- g -PS) was synthesized with high grafting percentage by utilizing the ionic liquid 1-ethyl-3-methylimidazolium acetate ([EMIM]Ac) as solvent and potassium persulfate as initiator. The effect of various parameters upon the polymerization were studied including: initiator concentration, styrene:starch weight ratio, the reaction time and temperature. Grafting percentages were calculated using an FT-IR calibration method, with values up to 114%. The resulting copolymer was characterized using FT-IR, SEM, WAXD and TGA, which demonstrated that polystyrene side chains were evenly distributed on the starch backbone. Our results indicate that ionic liquid dissolution of starch, prior to polystyrene grafting, is a versatile methodology for the synthesis of amphiphilic, polysaccharide-based graft copolymers, having high grafting percent.

LiTi2(PO4)3@carbon/graphene hybrid as superior anode materials for aqueous lithium ion batteries

Zhi Zhou,Wei Luo,Hao Huang,Shengxiong Huang,Yongji Xia,Nan Zhou,Zhangxing He

In this paper, LiTi 2 (PO 4 ) 3 @carbon/graphene (LCG) hybrid was prepared and investigated as anode for aqueous lithium ion batteries (ALIBs). The as-prepared hybrid was characterized by XRD , SEM, TEM and TGA . The electrochemical performances of nano-structured materials as anode were evaluated by cyclic voltammetry , electrochemical impedance spectroscopy and charge-discharge tests. The cell using LCG as anode exhibits excellent cycling performance both at relatively low and high rates. The capacity retention of LCG can remain 87.6% at 0.2   C after 200 cycles and 77.2% at 5   C after 1000 cycles compared with LiTi 2 (PO 4 ) 3 @carbon (0.2   C: 71.8%, 5   C: 58.1%). Moreover, LCG reveals superior rate capability with 80.8   mA   h   g −1 , 62.1   mA   h   g −1 at 10   C and 20   C, respectively. The outstanding electrochemical performance of LCG comes from the improved electrical conductivity and the forming of efficient conductive network. The results demonstrate that the graphene-modified LiTi 2 (PO 4 ) 3 @carbon is a promising anode for ALIBs.

Synthesis and luminescence properties of Eu3+, Dy3+ co-doped Ca3Bi(PO4)3 single-phase phosphor

Renping Cao,Caiping Chen,Fangrui Cheng,Ting Chen,Bang Lan,Lan Li,Ru Liu,Jing Wang

In this work, we synthesize Ca 3 Bi(PO 4 ) 3 :Dy 3+ , Ca 3 Bi(PO 4 ) 3 :Eu 3+ , and Ca 3 Bi(PO 4 ) 3 :Eu 3+ , Dy 3+ . The crystal structure, phase purity, and the luminescence properties of samples are investigated, respectively. Ca 3 Bi(PO 4 ) 3 :Dy 3+ under excitation at 350 nm shows yellow emission because of the 4 F 9/2  →  6 H 15/2 , 6 H 13/2 , 6 H 11/2 , and 6 H 9/2 transitions of Dy 3+ . Ca 3 Bi(PO 4 ) 3 :Eu 3+ with excitation at 394 nm emits red-orange emission due to the 5 D 0 → 7 F 1 , 7 F 2 , 7 F 3 , and 7 F 4 transitions of Eu 3+ ion. Ca 3 Bi(PO 4 ) 3 :Dy 3+ , Eu 3+ with excitation at 394 nm mainly shows the emission of Eu 3+ , however, that with excitation at 350 nm mainly derive from Dy 3+ ion. We investigate the effects of the ratio between Dy 3+ and Eu 3+ in Ca 3 Bi(PO 4 ) 3 :Eu 3+ , Dy 3+ on the luminescence properties. The energy transfer from Dy 3+ to Eu 3+ in Ca 3 Bi(PO 4 ) 3 :Eu 3+ , Dy 3+ is found. We analyze the luminous mechanism of samples by energy level diagrams of Dy 3+ and Eu 3+ ions. The results are helpful for the research of other Dy 3+ and Eu 3+ co-doped luminescence materials.

Identification and characterization of a novel high-activity amylosucrase from Salinispirillum sp. LH10-3-1

Li Jing,Li Ziyi,Gong Hui,Ma Mengyi,Li Shuolei,Yang Huilin,Zhang Hailin,Liu Jianguo

Abstract In this study, a novel high-activity amylosucrase from Salinispirillum sp. LH10-3-1 (SaAS) was identified and characterized. The recombinant enzyme was determined as a monomer with a molecular mass of 75 kDa. SaAS protein exhibited the maximum total and polymerization activities at pH 9.0 and maximum hydrolysis activity at pH 8.0. The optimum temperature for total, polymerization, and hydrolysis activities were 40, 40, and 45 °C, respectively. Under the optimal pH and temperature, SaAS had a specific activity of 108.2 U/mg. SaAS also showed excellent salt tolerance and could retain 77.4% of its original total activity at 4.0 M NaCl. The addition of Mg 2+ , Ba 2+ , and Ca 2+ enhanced the total activity of SaAS. When the conversion of 0.1 M and 1.0 M sucrose was catalyzed at pH 9.0 and 40 °C for 24 h, the ratios of hydrolysis, polymerization, and isomerization reactions were 11.9:77.4:10.7 and 15.3:53.5:31.2, respectively. The α-arbutin yield of 60.3% was achieved from 20 mM sucrose and 5 mM hydroquinone catalyzed by SaAS. Key points • A novel amylosucrase from Salinispirillum sp. LH10-3-1 (SaAS) was characterized. • SaAS has the highest specific enzyme activity among all known amylosucrase. • SaAS has hydrolysis, polymerization, isomerization, and glucosyltransferase activities. Graphical Abstract

Electrochemiluminescence Aptasensor Based on Gd(OH)3 Nanocrystalline for Ochratoxin A Detection in Food Samples

Chunyuan Tian,Minggang Wei,Xiaobin Wang,Qing Hua,Feiyan Tang,Lijun Zhao,Xuming Zhuang,Feng Luan

In the present study, the electrochemiluminescence (ECL) properties of Gd(OH)3nanocrystals with K2S2O8as the cathode coreactant were studied for the first time. Based on the prominent ECL behavior of this material and the excellent specificity of the aptamer technique, an ECL aptasensor for the detection of ochratoxin A (OTA) was formulated successfully. Over an OTA concentration range of 0.01 pg mL−1to 10 ng mL−1, the change in the ECL signal was highly linear with the OTA concentration, and the limit of detection (LOD) was 0.0027 pg mL−1. Finally, the ECL aptasensor was further used to detect OTA in real samples (grapes and corn) and satisfactory results were obtained, which indicated that the built method is expected to be applied in food detection.Keywords:Gd(OH)3nanocrystals;electrochemiluminescence;aptasensor;ochratoxin A;food detection