JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2025
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IF 3.5
Haoming Guo,Nan Xiao,Yabo Liang,Xueke Liu,Peng Wang,Donghui Liu
BACKGROUND The threat to bees of neonicotinoid pesticides (NEOs) has raised concern. However, data on the disruption of the bee nervous system following long-term low-dose exposure are lacking. In this study, six NEOs were quantified in 31 honey samples from China, and their behavioral effects on bees were evaluated. RESULTS Neonicotinoid pesticide detection rates ranged from 35.48% to 83.87%, with mean residue concentrations of 2.39–6.73 μg kg⁻¹. Clothianidin showed the highest detection frequency (83.87%), with an average residue of 5.54 μg kg −1 . To assess neurotoxic effects at actual residue levels, bees were fed NEO-contaminated syrup for 21 days. This exposure significantly downregulated eight neurotransmitters, particularly 5-hydroxytryptamine (5-HT) and tryptophan (Trp), impairing normal physiological function. Behavioral assays showed reductions of 31.71% to 68.53% in displacement distance, 27.13% to 40.06% in movement rate, and 64.58% to 77.14% in inter-individual contact time. CONCLUSION This study confirmed that the NEO residues in honey could hinder bees' ability to communicate and forage, with potential adverse effects on the development of bee populations. It demonstrated that bees exposed to real-world residue levels of NEOs exhibit neurological impairments. © 2025 Society of Chemical Industry.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2024
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IF 4.3
Gan Wu,Zhaoyang Ding,Xilin Dou,Ze Chen,Jing Xie
Histamine has been known as a momentous cause of biogenic amine poisoning. Therefore, the content of histamine in foods is strictly required to be controlled within a certain range. Here, an aptamer fluorescent sensor was developed for detection of histamine. Poly [(9, 9-di-n-octylfluorenyl-2, 7-diyl)-alt-(benzo [2,1,3] thiadia-zol-4, 8-diyl)] (PF8BT) and the styrene maleic anhydride copolymer (PSMA) were used for the preparation of PF8BT-Polymer dots (PF8BT-Pdots). PF8BT-Pdots and the cyanine3-phosphoramidite (Cy3) were linked through aptamer to achieve the ratiometric detection for histamine. PF8BT-Pdots were partly quenched by Cy3 due to the fluorescence resonance energy transfer (FRET), when the histamine molecule was recognized by aptamer on the surface of PF8BT-Pdots. A linear range (3–21 μmol/L) was obtained for histamine detection with a low limit of detection (LOD = 0.38 μmol/L). PF8BT aptamer Pdots (PF8BT-A) were used to detect histamine in simply treated aquaculture water and tuna. The cell imaging of HeLa cells presented a good biosecurity and outstanding fluorescent imaging capability of PF8BT-A. The aptamer fluorescent sensors provided a new platform for rapid and accurate detection of histamine in aquatic products and had great potential for the application in food safety and quality control.
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BIOTECHNOLOGY AND BIOENGINEERING
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2025
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IF 3.6
Man Zhao,Mengying Yu,Huiru Yuan,Yiting Shen,Zhiqiang Liu,Yuguo Zheng
Carcinine, a valuable imidazole dipeptide with antioxidant and therapeutic properties, faces biosynthesis challenges due to enzyme aggregation and substrate inhibition. In this study, an integrated strategy combining linker peptide engineering and immobilization was applied to address these challenges and enhance carcinine production. Rational design of linker peptides (D 5 , L 2 , L 3 ) in the sfp-Ebony fusion protein enabled its highest soluble expression in WSL 2 E strain, achieving 93.1% conversion efficiency—3.5-fold higher catalytic efficiency than WSGE strain. Response surface methodology optimized sodium alginate-polyvinyl alcohol (SA-PVA) immobilization parameters (5% PVA, 3% SA, 2.3% CaCl₂), yielding excellent immobilized WSL 2 E@SA-PVA cells with 95.93% activity recovery. Structural characterization by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) confirmed the formation of a porous SA-PVA matrix that protected cells from harsh conditions. The immobilized biocatalyst exhibited superior operational stability (retaining > 80% activity after 7 cycles) and storage stability (maintaining 44.89% activity after 14 days at 4°C). Fed-batch scale-up (50 mL) achieved a record carcinine titer of 71.13 mM, mitigating the inhibitory effect of high substrate concentrations through phased substrate feeding. This study provides a scalable biocatalytic platform for industrial carcinine production, effectively addressing key bottlenecks in biocatalyst stability and substrate tolerance.
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FOOD CHEMISTRY
·
2025
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IF 9.8
Chunfeng Zhang,Yuandong Lin,Kevin M. Keener,Jun-Hu Cheng
The exploration of cost-effective methods for monitoring aquatic products freshness is significant. Metal nanoclusters (MNCs) show considerable potential in detecting food spoilage. A ligand modification strategy has been proposed as an effective approach for preparing high-performance MNCs. In this study, polyvinylpyrrolidone (PVP) and methylthioxanthine (MTU)-capped copper nanoclusters (PVP-MTU-CuNCs) with high quantum yield (43.76 %) were synthesized using an eco-friendly one-pot method. PVP improved the electron transfer and restricted the motion of MTU, enhancing photoluminescence, while its long-chain formed a physical barrier, effectively improving dispersibility and stability. PVP-MTU-CuNCs exhibited a satisfactory responsiveness to volatile basic nitrogens (VBNs), with detection limits of 0.34, 0.36, 0.33, 3.23, and 3.45 ppm for spermine, spermidine, histamine, cadaverine, and ammonia, respectively. An intelligent fluorescent label was developed for ammonia sensing and achieved visual semi-quantification and real-time tracking of the freshness of sea bass and shrimp, demonstrating its practical potential in assessing aquatic products freshness
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MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Lingcan Kong,Haixin Chen,Qiaoyan Liu
Excessive intake bioamines is harmful to human health, and thus the development of simple, rapid and efficient detection method of bioamines in food is urgent. Herein, an unprecedented method based on a commercial molecular sieve has been developed for the determination of four bioamines (cadaverine, tyramine, histamine and tryptamine) in fruit wines by dispersive solid phase microextraction (D-μSPE) coupled to liquid chromatography-triple quadrupole tandem mass spectrometry (LC-MS/MS). The extraction process of ultra-stable Y-type molecular sieve (USY) on bioamines belongs to physisorption, and the adsorption pattern is found in good accord with the Langmuir isotherm model. Theoretical and experimental analyses are further used to explore their binding energies, binding constants, and the maximum adsorption capacity. It is found that the adsorption is driven mainly by hydrogen bonds between USY and bioamines and water molecules. Moreover, the extraction efficiency is dependent on the binding constants and the maximum adsorption capacity of USY. The optimization of adsorption-desorption process involves the USY amount, extraction time, desorption solvent, desorption time, desorption number and cyclic usage number of USY. Under the optimum conditions, the limits of detection and quantification were found in the range of 0.2–2.5 μg L −1 and 0.6–8 μg L −1 with intra-day and inter-day precision and recoveries in a fruit wine in the range of 1.3 %–7.9 % and 91 %–104 %, respectively. Owing to the efficient enrichment and edulcoration functions of USY, the method shows negligible matrix effect and is simple, rapid, and has the potential to apply in routine work.
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AMB Express
·
2021
·Vol. 11
·期 1
·1-11
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IF 3.298
Zhao Yirui,Sang Xue,Hao Hongshun,Bi Jingran,Zhang Gongliang,Hou Hongman
Controlling the content of biogenic amines (BAs) is critical to guarantee the safety of fermented aquatic products. The degradation characteristics and application potential of amine-negative starter cultures ( Virgibacillus halodenitrificans CGMCC 1.18601: G25 , Virgibacillus pantothenticus CGMCC 1.18602: G38) screened from grasshopper sub shrimp paste (Gssp) were studied. The enzymes of the two strains G25 and G38 that degrade BAs were amine oxidases (AOs) located on their respective cell membranes. The conditions that promoted the AO activity of Virgibacillus spp. were NaCl concentrations 5–10%, temperature 37 °C, pH 7.0 and ethanol concentrations 0–2%. Safety assessments (antibiotic susceptibility, biofilm activity and hemolytic activity) indicated that Virgibacillus spp. do not present a risk to human health, and this isolate can be confidently recommended as safe starter cultures for the food industry. Then, the two strains were cultured separately as starters and applied to the Gssp to analyze their influence on the flavor and quality of the product. As far as the bad flavors in Gssp such as sulfur-organic and sulf-chlor were concerned, the response values in the starter groups by G25 and G38 were significantly reduced by 39% and 65%, respectively. For the ability of strains to degrade BAs in Gssp, G25 degraded 11.1% of histamine, 11.3% of tyramine, 15.5% of putrescine and 4.1% of cadaverine; G38 significantly degraded 10.1% of histamine, 21.8% of tyramine, 18.1% of putrescine and 5.0% of cadaverine. These results indicated that the selected species could be used as starter cultures for the control of BA accumulation and degradation in Gssp.
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FOOD CHEMISTRY
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2025
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IF 9.8
Li-Jian Chen,Jiang-Yue Wang,Xu Zhao,Xiu-Ping Yan
A ratiometric fluorescent hydrogel microneedle (MN) patch was developed for rapid raw meat freshness detection. The sensor integrated fluorescein isothiocyanate (FITC) as a biogenic amine (BAs) indicator and rhodamine B (RhB) as an internal reference within a silk fibroin methacryloyl (SilMA) hydrogel matrix. Leveraging FITC's specific response to BAs, the patch exhibited visible color changes proportional to histamine concentrations. Optimizing FITC/RhB concentrations enabled sensitive BAs detection in meat samples within 8 min. The FITC/RhB@SilMA MN patch demonstrated high accuracy and repeatability in freshness assessment for chicken breast, pork, and salmon stored at 0 °C and 4 °C. Freshness classification was established using G + R/(R + G + B) values: <0.60 (fresh), 0.60–0.65 (low quality), >0.65 (spoiled). These values showed strong correlation with the Biogenic Amine Index (BAI), confirming method reliability. This work presents a novel on-site strategy for real-time meat quality monitoring, offering practical applications in food safety control.
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Smart Medicine
·
2025
·Vol. 4
·期 1
·e136
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IF 11.6
Yuwei Wang,Lijun Cai,Yuanyuan Zhang,Yan Cong,Yuanjin Zhao
Allergic contact dermatitis (ACD) is an inflammatory dermatitis with a high morbidity and recurrence rate. Scientific attention is focused on the development of safe and comfortable therapeutics of ACD. Herein, we propose a natural matrine-integrated pollen delivery system for the ACD treatment. Sunflower pollens were collected and defatted to serve as adhesive drug carriers for matrine. Specifically, the exquisite porous and hollow structures of the pollen shells can absorb matrine and realize the sustained drug release. Besides, the prickly surface morphology can strongly adhere to the inflamed skin sites, which can prolong the duration of the drug. By utilizing them in an ACD model and an acute pruritus model of mice, we have demonstrated that these matrine-integrated pollen shells can decrease the swelling degree of mice ears and weight loss, down-regulate inflammatory response, and improve the scratching times. These results indicate that our matrine-integrated pollen delivery systems have great potential to serve as natural topical preparations for skin disease therapy.
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Nature Communications
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2025
·Vol. 16
·期 1
·1-17
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IF 15.7
Wen Zhenzhen,Liu Tong,Xu Xutao,Acharya Nandini,Shen Zhida,Lu Yunkun,Xu Junjie,Guo Ke,Shen Shuying,Zhao Yuening,Wang Pinli,Li Shumin,Chen Weiyu,Li Hui,Ding Yimin,Shang Min,Guo Hongshan,Hou Yu,Cui Bijun,Shen Manlu,Huang Youling,Pan Ting,Qingqing Wang,Cao Qian,Wang Kai,Xiao Peng
Overcoming immunosuppression in the tumor microenvironment (TME) is crucial for developing novel cancer immunotherapies. Here, we report that IL-16 administration enhances the polarization of T helper 1 (Th1) cells by inhibiting glutamine catabolism through the downregulation of glutaminase in CD4 + T cells and increases the production of Th1 effector cytokine IFN-γ, thus improving anti-tumor immune responses. Moreover, we find that establishing an IL-16-dependent, Th1-dominant TME relies on mast cell-produced histamine and results in the increased expression of the CXCR3 ligands in tumor-associated macrophages (TAM), thereby improving the therapeutic effectiveness of immune checkpoint blockade (ICB). Cancer patients exhibit impaired production of IL-16, which correlates with poorer prognosis. Additionally, low IL-16 production is associated with unresponsiveness to immunotherapy in cancer patients. Collectively, our findings provided new insights into the biological function of IL-16, emphasizing its potential clinical significance as a therapeutic approach to augment anti-tumor immunity and sensitize ICB-based cancer immunotherapy.
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MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Yan Zhou,Jinlong Wang,Wangni Meng,Lian Xia,Yue Tang,Jianli Zhou,Yuangen Wu,Pengcheng Yan
Acetamiprid (ACE), a neonicotinoid pesticide broadly used in agricultural production, can accumulate in the food chain due to excessive or incorrect use, thereby posing a threat to human health. In this manuscript , a colorimetric aptasensor assisted by a smartphone was established, relying on the S-18 aptamer-mediated amplification of the laccase-mimic activity of spherical Ag 2 O nanoparticles (NPs). The S-18 aptamer strengthens the interaction and facilitates electron transfer between the Ag 2 O NPs and oxidative substrates, which significantly improves the laccase-mimic activity of the Ag 2 O NPs. When acetamiprid is present, the S-18 aptamer preferentially binds to the target molecule, forming a steady aptamer-acetamiprid complex. This binding interaction prevents the aptamer from promoting the catalytic behavior of the Ag 2 O NPs, producing a distinct and quantifiable color variation in the reaction system. Monitoring the concentration-dependent colorimetric response enables the quick and accurate identification of acetamiprid. The colorimetric aptasensor achieved a limit of detection (LOD) of 8.69 μg∙L −1 markedly lower than the maximum residue limits defined by the national standard (GB 2763–2019) for acetamiprid in fruits and vegetables. Furthermore, the aptasensor demonstrates satisfactory recovery rates in actual samples analyses of cabbage, apple, and tomato, confirming its practical potential for on-site food safety oversight.
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TALANTA
·
2025
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IF 6.1
Lian Xia,Jia Zheng,Jian Su,Yue Tang,Zhen Wu,Xiaojuan Niu,Yuangen Wu
Amygdalin, a cyanogenic glycoside abundant in apricot kernels, poses a food safety risk, requiring rapid detection that conventional chromatographic methods cannot fulfill for on-site application. Thus, this study aims to construct a novel colorimetric sensing platform and improve detection performance through a multivalent aptamer strategy. This pioneering work introduces a novel bivalent aptamer strategy for ultra-sensitive on-site amygdalin detection. The aptamers specific to amygdalin were screened using a five-segment single-stranded DNA library and Capture-SELEX, with truncated variants optimized to obtain a high-affinity core sequence. A key innovation involves the first design and synthesis of a bivalent aptamer that exploits synergistic binding to enhance target affinity. The bivalent aptamer (Bapt 4-4) exhibited doubled affinity for amygdalin compared to its monovalent truncated counterpart (Amyg 4–26), achieving a dissociation constant ( K d ) of (2.19 ± 1.03) nM. By integrating the bivalent aptamer with the laccase-like activity of silver oxide nanoparticles (Ag 2 O NPs), an aptasensor with high sensitivity and specificity was developed, yielding a detection limit of 0.008 μg mL −1 , which is lower than that of the monovalent aptamer-based sensor (0.0137 μg mL −1 ). This multivalent aptamer strategy provides an effective approach for highly sensitive amygdalin detection. Furthermore, the constructed bivalent aptasensor provides a robust technical tool for the on-site monitoring of plant-derived cyanogenic glycoside toxins, with broad application prospects in the domains of food safety and traditional Chinese medicine safety.
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