CERAMICS INTERNATIONAL
·
2023
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IF 5.2
Hongyan Chen,Zi-yang Huang,Wenjun Chen
Hydroxyapatite (HA), chitosan (CS), ZrO 2 and MgO suspensions were distributed by zonal electrophoresis (ZE), and then HA/CS/ZrO 2 /MgO composite coating on the surface of titanium substrate was fabricated by electrophoretic deposition (EPD) in a reverse electric field subsequently. An HA/ZrO 2 /MgO gradient composite coating ( Z1 ) was fabricated by calcination at 700 °C for 120 min. At the same time, another HA/ZrO 2 /MgO composite coating ( E1 ) was directly fabricated only by electrophoretic deposition for comparison, where ZrO 2 and MgO were osteogenic trace element compounds (OTECs). The morphology, structure, composition, wettability , apatite-forming ability, antibacterial efficacy of the composite coating Z1 were investigated by XRD , FT-IR, FESEM, EDS, contact angle goniometry, Lloyd's universal testing machine, electrochemical workstation, immersing in simulated body fluid, and inhibition experiments. The results showed that the contents of Ca, Zr, and Mg elements had a gradient distribution in direction of coating thickness. With the addition of ZrO 2 and MgO, the contact angle of Z1 is significantly reduced from 52.5° to 27.3°. Remarkably, Z1 has more improved properties than E1 , mainly manifested in the bonding strength of Z1 and titanium substrate up to 29.3 MPa, exceeding the standard requirements for bonding strength of HA coating implants (15 Mpa, iso13779-2) and as can be observed from cyclic voltammetry (CV) curve and Tafel polarization curve, Z1 exhibit superior electrochemical stability and strong corrosion resistance when immersed in HEPES simulated body fluid (H–SBF). In addition, after immersing in H–SBF for 24 d, the surface of the Z1 was completely covered by carbonated hydroxyapatite (CHA) and the Ca/P of the coating increased from 1.67 to 1.99. Furthermore, the antibacterial effect test of Z1 showed that the antibacterial rates of Z1 against Escherichia coli and Staphylococcus aureus reached to 98.2 % and 86.3 %, respectively.
研究领域:
材料科学
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生物医学工程
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产品关联
DOI
高影响因子
LANGMUIR
·
2025
·
IF 3.9
Ning Guan,Qin Chen,Xinyue Zhang,Kai Chen,Haiyan Feng,Cunao Feng,Dekun Zhang,Shirong Ge
Polyether ether ketone (PEEK) is widely used in orthopedics due to its good mechanical properties and biocompatibility; however, its bioactivity and osseointegration ability are poor. In this article, PEEK materials were dual-modified by surface sulfonation treatment and the loading of active hydrogels (phosphorylated gelatin/chitosan/hydroxyapatite) to improve their bioactivity and osseointegration. Initially, a microstimulated sulfonation process was employed to construct an optimized porous structure on the PEEK surface, with a porosity of 54.63% and an average pore size of 3.38 μm, which significantly improved hydrophilicity and promoted the adhesion and growth of osteoblasts. Subsequently, utilizing the adhesive properties of polydopamine (PDA), the phosphorylated gelatin/chitosan/hydroxyapatite composite hydrogel (PG/CS/HA hydrogel) was loaded onto the surface of SPEEK, resulting in the construction of a highly active and strongly osteogenic SPEEK-PG/CS/HA material. The results showed that the dual modification of the porous sulfonated structure and bioactive hydrogel significantly enhanced the hydrophilicity, biocompatibility, mineralization ability, and osseointegration of the PEEK. Specifically, the cell proliferation capacity increased by 32% after 7 days of coculture on MC3T3-E1 cells; the alizarin red staining rate after 14 days of coculture reached 72.49%, representing a 111% improvement; and the expression levels of osteogenic genes ALP, OPN, and RUNX2 increased by 132%, 151%, and 131%, respectively, demonstrating excellent in vitro osteogenic induction potential. The SPEEK-PG/CS/HA material effectively solves the problem of bioinertia in the PEEK material, offering a novel modification strategy for orthopedic implants.
研究领域:
生物医学工程
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生物材料
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组织工程
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骨科
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表面改性技术
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Yingru Huang,Jiarui Ren,Yufeng He,Xiangbin Sun,Zhiyan Ma,Rongmin Wang
The overused of antibiotics had led to major environmental issues and drug-resistant bacteria, which inevitably harmed the environment and public health. In this work, in order to obtain environmentally friendly bi-functional materials to combat with residual antibiotics and bacterial contamination, ZIF-8, a typical Zn based zeolitic imidazolate framework (ZnZIF) with excellent chemical stability, was conjugated to hydroxyapatite nanowire bundles (HAnBs) by a simple in-situ synthesis. The obtained ZnZIFs@HAnBs was characterized by SEM, EDS, XPS, FT-IR, XRD and BET. The effects of catalysts, catalyst dosage, TC initial concentration, PMS concentration and pH on TC degradation were investigated. Notably, ZnZIFs@HAnBs (94.0%) exhibited excellent degradation effect of tetracycline (TC) within 15 min, which was 1.7 and 5.2 times than that of ZIF-8 (55.9%) and HAnBs (18.1%) under optimal conditions, respectively. Moreover, after recycling 10 times, the degradation rate still more than 86.8%. The degradation mechanism was confirmed by free radical quenching experiment coupling with electron paramagnetic resonance (EPR), and the 1 O 2 and O 2 •- were the major reactive oxygen species in TC degradation. Furthermore, it presented excellent antibacterial activity (95.0%) against E. coli and S. aureus , which was attributed to the synergistic effect of sustained release of Zn 2+ and phosphate in ZnZIF@HAnBs. In summary, an eco-friendly bi-functional materials of HA nanowire bundles could be synthesized by simple strategy, and it could be applied in the fields of environmental remediation, as well as in treatment of antibiotic and bacterial pollution.
研究领域:
环境科学
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材料科学
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化学工程
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产品关联
DOI
高影响因子
JOURNAL OF THE EUROPEAN CERAMIC SOCIETY
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2024
·
IF 5.8
Chang Peng,Ming-Zhu Pan,Jia-Min Wu,Jin-Feng Yan,Qian-Xun Liu,Xiao-Yan Zhang,Xiu-Mei Wang,Yu-Sheng Shi
Bone defects resulting from bone tumor resections are often complicated with residual cancer cells. To address the challenge, the magnetothermal porous structured bone scaffolds of hydroxyapatite-Fe 3 O 4 -MgO (HA-FM) were fabricated by Vat Photopolymerization (VPP). This study improved curing behavior by doping Mg(OH) 2 into Fe 3 O 4 powder via the chemical deposition method. Mg(OH) 2 functioned as a pore-forming agent during the sintering process, decomposing into MgO to enhance biocompatibility. A two-step debinding method combined with a carbon powder embedding sintering process was used employed to resolve the conflict between the removal of photosensitive resin and the oxidation of Fe 3 O 4 . After sintering at 1200°C, the porosity of the composite ceramics reached 69% and a compressive strength of 2.28 MPa. In vitro mineralization tests showed that doping with Fe 3 O 4 and Mg(OH) 2 could promote the scaffolds’ degradation in simulated body fluid (SBF), beneficial to mineralization process. In vitro cell proliferation and adhesion experiments showed that ceramic samples were not cytotoxic and could promote osteogenic differentiation. The composite scaffolds exhibited magnetothermal properties, achieving a temperature increase of 8.2°C in the alternating magnetic field of 92 G and 100 kHz, indicating potential for cancer treatment.
研究领域:
材料科学
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生物医学工程
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癌症治疗
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生物材料
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组织工程
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2025
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IF 5.7
Haowen Zhong,Zongyou Chen,Jiahao Huang,Xiao Yu,Chengyong Wang,Yue Zheng,Mengran Peng,Zhishan Yuan
High incidence and mortality rates of chronic wounds place a heavy burden on global healthcare systems. Achieving phased delivery of antimicrobial and regenerative drugs is crucial for promoting chronic wound healing. Herein, a microneedle (MN) patch with a biphasic release system was developed using a combination of solvent casting and spraying methods. Additionally, a copper/PDMS mold was introduced to address the issue of deformation in the chitosan material during drying on polydimethylsiloxane (PDMS). The MNs have a bilayer structure, with a hyaluronic acid (HA) coating loaded with doxycycline (DOX) for antibacterial action and a chitosan (CS) core loaded with vascular endothelial growth factor (VEGF) for promoting cell migration and proliferation. Notably, in vitro drug release studies showed that the coating drug was released by 98.8% within 10 hours, while the release of the core drug could be sustained for up to 70 hours. In vivo studies showed that chronic wounds on C57 mice treated with CS/HA-bilayer MNs achieved nearly complete healing by day 9. These wounds exhibited reduced inflammatory cell infiltration, increased epithelial tissue regeneration, and enhanced collagen deposition. This work integrates the staged management of bacterial infection and angiogenesis and offers promising prospects for enhancing chronic wound healing.
研究领域:
生物医学工程
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药剂学
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组织工程
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产品关联
DOI
PubMed
高影响因子
Materials Today Chemistry
·
2025
·
IF 6.7
Rui Zhang,Meilan Liu,Lin Bao,Huanchen Yan,Huahui Feng,Danyi Huang,Ying Ding,Min Chen
Chondrodysplasia presents with abnormal chondrocyte differentiation and altered cartilage extracellular matrix, which restricts cartilage and bone growth. Kartogenin (KGN) is recognized as a chondroinductive molecule, whereas the limitation of hydrophobicity and low bioavailability are obstacles to therapy. Nanocarriers for drug delivery can potentially defeat some of the challenges of drug delivery of hydrophobic species. Herein, ROS-responsive core-shell codelivery nanocarriers were developed for chondrogenesis with a core consisting of mesoporous silica nanoparticles (MSN) loaded with KGN and embedded with ultrasmall Prussian blue (USPB), and a shell consisting of selenized hyaluronic acid (Se-HA). MKP@Se-HA nanoparticles effectively improved the bioavailability of KGN. The embedded USPB enhanced structural stability and antioxidant properties, while Se-HA coating targeted chondrocytes via CD44 and employed diselenide bonds as ROS-responsive to achieve KGN release at lesion sites. In vitro and in vivo experiments demonstrated that MKP@Se-HA nanoparticles could promote chondrocyte proliferation and differentiation by upregulating SOX9 and Col II expression. Meanwhile, MKP@Se-HA nanoparticles had a positive effect in the zebrafish chondrodysplasia model by modulating the formation of cartilage matrix and reducing apoptotic cells. Overall, the prepared core-shell codelivery nanoparticles offer a promising strategy for improving chondrodysplasia by synergistically promoting chondrocyte proliferation and differentiation.
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产品关联
DOI
高影响因子
MATERIALS TECHNOLOGY
·
2020
·
IF 1.738
Ruonan Zhang,Leilei Zhang
To improve the surface bioactivity of carbon/carbon composites, functionalized carbon nanotube-gelatin-Zn doped hydroxyapatite (FIC-GL-ZnH) composite coatings were fabricated on carbon/carbon composites by a combination method of chemical vapor deposition, soaking and electrochemical deposition method. The morphology, bonding strength and in-vitro bioactivity of composite coating were investigated. Results showed that the functionalized carbon nanotube-gelatin could not only bond with the carbon/carbon composite substrate tightly by in-situ grown mode but also provide a three-dimensional network structure for the infiltration of Zn doped hydroxyapatite. The FIC-GL-ZnH coating with spherical shape had excellent bonding strength, which was about 2.3 times higher than that obtained for Zn doped hydroxyapatite coating. In-vitro bioactivity test demonstrated that the FIC-GL-ZnH coating could induce the formation of bone-like apatite with particle shape, exhibiting good in-vitro bioactivity. It was suggested that the FIC-GL-ZnH coating might be an effective method to improve the surface bioactivity of carbon/carbon composites.
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DOI
高影响因子
TISSUE & CELL
·
2020
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IF 1.837
Biao Hu,Zeng-Dong Meng,Yu-Qin Zhang,Li-Yuan Ye,Cheng-Jian Wang,Wei-Chun Guo
Background Ideal bone defect repair scaffolds should be biodegradable, biocompatible, bioactive, porous, and provide adequate mechanical support. However, it is challenging to fabricate such an ideal bone repair scaffold. Previously, we showed that 5 wt.% strontium-doped hydroxyapatite (Sr-HA) scaffolds prepared by spark plasma sintering (SPS) technology exhibited good biocompatibility. Moreover, unlike pure hydroxyapatite (HA) scaffolds, HA scaffolds containing strontium (Sr) exhibited superior bioactivity, higher proliferation rate of BMSCs and MG-63 osteoblast cells, as well as enhanced BMSCs differentiation. Methods In this study, we prepared pure HA scaffolds and 5 wt.% strontium containing Sr-HA scaffolds by SPS technology without adhesive, ammonium bicarbonate as pore former. Subsequently, scanning electron microscope (SEM) and X-Ray diffraction (XRD) were used to characterize the properties of Sr-HA and HA scaffolds. The ability of the scaffolds to repair bone defects was evaluated using a critical-sized rabbit tibia-bone defect rabbit model. Thirty 3-month-old New Zealand white rabbits were randomly divided into three groups (blank control group, Sr-HA scaffolds implanted group and HA scaffolds implanted group) with 10 rabbits in each group. These rabbits are sacrificed after 8 weeks and 16 weeks of surgery, and the repair effects of each scaffold were evaluated with X-ray, micro-CT, and HE staining. The three-point bending test was employed to assess the mechanical property of repaired bones. Results XRD pattern indicated that Sr-HA and HA scaffolds possess a similar crystal structure after sintering, and that incorporation of strontium did not form impure phase. SEM showed that the porosity of Sr-HA and HA scaffolds was about 40 %. Universal Testing Machine tests showed that Sr-HA scaffolds had better compressive strength than HA scaffolds. Bone defect was obvious, and the fibrous tissue was formed in the bone defects of rabbits in the blank control group after 8 weeks of surgery. Sr-HA and HA scaffolds enhanced osteointegration of the host bone, and extensive woven bone was formed on the surface of the Sr-HA scaffolds. After 16 weeks, the bone strump became blunt and a small amount of callus was formed in the blank control group. Comparatively, the scaffolds were substantially degraded in the Sr-HA scaffolds implanted group while scaffolds shadows still were observed in the HA implanted group. Bone remodeling and cavity recanalization were completely developed in the Sr-HA scaffolds group. The compressive strength of repaired bone in the Sr-HA scaffolds implantation group was higher than that of HA scaffolds implantation group after 8 weeks and 16 weeks of surgery. Conclusions Our results show that the Sr-HA composite scaffolds can effectively repair bone defects and have good biodegradable properties.
研究领域:
生物材料学
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骨组织工程
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生物医学工程
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再生医学
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生物力学
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口腔颌面外科
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纳米医学
✓ 已验证
DOI
高影响因子
MATERIALS LETTERS
·
2012
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IF 2.307
Quan Wu,Xianglin Zhang,Bin Wu,Wei Huang
The minimal demands for scaffolds include a guaranteed strength over time. In this study, a novel porous HA/β-TCP bioceramic scaffold with micro-ribs structure was fabricated by extrusion deposition technique and microwave sintering. Micro-ribs were placed at the center and corners of scaffold along the direction of load. Mechanical behaviors were studied to verify the strengthening effect of micro-ribs. Compared to the scaffold without micro-ribs, the average compressive strength of newly developed scaffold was remarkably improved from 28.3 MPa to 45.6 MPa under the porosity of 50%. Moreover, it also exhibited more stable and longer lasting mechanical strength during degradation in vitro. The effectiveness of micro-ribs on improving the mechanical performance of scaffolds provided a structural design reference for bone tissue engineering.
研究领域:
生物材料学
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骨组织工程
✓ 已验证
产品关联
DOI
高影响因子
Asian Journal of Pharmaceutical Sciences
·
2021
·
IF 6.598
Chaoqun Ma,Yichao Duan,Chaohui Wu,Erjuan Meng,Pingping Li,Zhenzhong Zhang,Chunhua Zang,Xueling Ren
There is growing empirical evidence that certain types of chemotherapy and phototherapy trigger immunogenic cell death and enhance the therapeutic anticancer efficacy of genetic immunotherapy. However, the main challenge is spatiotemporally co-delivering different drugs to maximize the therapeutic index of the combination therapy. In this study, a drug delivery system (HTCP-Au/shPD-L1/DOX) was designed with a polysaccharide-wrapped shell and a condensed DNA core. To construct the HTCP-Au vector, dodecyl side chains with a polyethylenimine (PEI) head were grafted onto hyaluronic acid, and AuNPs were grafted via Au-S bonds. During drug loading, PEI arrested shRNA plasmid DNA targeting programmed cell death ligand 1 (shPD-L1) via electrostatic interactions. It also formed a PEI-DNA core that was automatically enclosed when aliphatic hydrocarbons pulled the hyaluronic acid backbone. A hydrophobic interlayer consisting of dodecyl bridge chains between the PEI-DNA core and the hyaluronic acid shell was required to accommodate hydrophobic doxorubicin. In vitro and in vivo assays demonstrated that this core-shell drug delivery system could efficiently load and transport three different drugs and effectively target tumors. Moreover, it could activate the immune system, thereby providing promising therapeutic efficacy against tumor growth and metastasis.
研究领域:
癌症治疗
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纳米技术
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药物递送
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免疫疗法
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SURFACE & COATINGS TECHNOLOGY
·
2020
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IF 3.784
Cong Wu,Yufei Tang,Bobo Mao,Kang Zhao,Siyue Cao,Zixiang Wu
Coatings with piezoelectric characteristics were prepared on the titanium alloy's surface to improve its biological properties, so that the titanium alloy possessed a piezoelectric effect similar to that of bones, thereby promoting bone growth and shortening bone repair time. Compared with other piezoelectric ceramic coatings , PVDF bio-piezoelectric coatings have better piezoelectric properties. However, the PVDF coating is hydrophobic, which is not conducive to calcium deposition. In this study, HA/PVDF coating was obtained on the titanium surface, which indicated a piezoelectric effect. The hydrophilicity of the coating has been improved with the increase of HA content. Corona poling gives the HA/PVDF coating piezoelectric characteristics, and also effectively reduces the contact angle. Moreover, the contact angle of the polarized HA/PVDF coating decreased by 66.9%. The synergistic effect of the introduction of HA and the high piezoelectric coefficient promotes the coating's mineralization. Surprisingly, the poled 20HA/PVDF coating surface was entirely covered by apatite after soaking in simulated body fluids (SBF) for 1 day with physiological loading. Additionally, the coating with rapid apatite deposition ability allows titanium alloy implants to obtain excellent biological activity, which has potential application value in the rapid repair of bone defects.
研究领域:
生物材料学
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骨科医学
✓ 已验证
DOI
高影响因子
Scientific Reports
·
2018
·Vol. 8
·期 1
·1-8
·
IF 4.122
Li Xiyu,Zou Qin,Li Wei,Chen Haifeng
Hydroxyapatite (HA) material will be long-standing once implanted in bone tissue of the body. It should be considered to endow the osteogenic HA material with traceable fluorescence to realize a lifelong in vivo tracking. We prepared and utilized lanthanides-doped HA upconversion material, and revealed for the first time that the lanthanides (ytterbium (Yb) and holmium (Ho)) co-doped HA upconversion material was suitable for long-term or lifelong in vivo tracking, the lanthanide ions doped in the HA matrix would not affect the biocompatibility and osteogenesis, and the tissue autofluorescence could be effectively avoided by the HA:Yb/Ho upconversion material. Also the distribution in bone and osteointegration with bone of the HA:Yb/Ho material could be clearly discriminated by its bright fluorescence under NIR irradiation. The upconversion characteristic of the HA:Yb/Ho material provides a feasibility and promising prospect for lifelong in vivo tracking, and has an advantage in revealing the material-tissue interrelation. The material has important clinical application value in addition to its usefulness for scientific investigation.
研究领域:
生物医学工程
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材料科学
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骨科研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）