Journal of Nanoelectronics and Optoelectronics
·
2018
·Vol. 13
·期 7
·1041-1047
·
IF 1.069
Dong Yuhuan,Ma Feng
A type of nanofiber membrane of polyvinyl butyral (PVB)/OP-10/lipase was prepared using electrostatic spinning technology. The results of environmental scanning electron microscopy (ESEM) indicate that the microscopic morphology of PVB/OP-10/lipase membrane spun under 14 kV of voltages,
10 %wt concentration of emulsifier (relative to the amount of PVB) and 10 cm of the distance from the receiver to the needlepoint is uniform. The stability of immobilized and free lipase was studied under different pH, voltages and temperature and storage time. It is showed compared with the
free enzyme, the immobilized enzyme has better temperature-resistant and better storage stability. The immobilized enzyme has recycling activity of 65% after 10 times uses. The esterification rate of lauric acid with lauryl alcohol catalyzed by immobilized lipase can get to 72% after 20 hours
of reaction. While the reaction of lauric acid with lauryl alcohol catalyzed by free lipase cannot occur.
研究领域:
生物化学
·
材料科学
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DOI
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2010
·
IF 2.193
Gangfeng Ren,Hongwei Yu
After analyzing protein surface structure, the opposite side of the activity site of esterase BioH was observed to be hydrophilic. Hence subsequently, a hydrophilic-modified solid support was selected to immobilize enzyme through oriented adsorption. The immobilized enzyme remained 86% of its original activity under the acylation of 1-phenylethanol, much more than those immobilized on other solid supports with low hydrophilicity (20–35.6%), as the mode of the immobilization could facilitate enzyme to expose its active site to the substrate. This demonstrated that the strategy to select solid support based on the analysis of protein surface structure was successful. It was also noticed that the substrate of the reaction was obviously adsorbed onto the support, therefore the adsorption effect could not be ignored and it was necessary to obtain the adsorption isotherm of the substrate for a more authentic result. The parameter K m shifted from 884.56 to 296.09 mM after immobilization. This was possibly mainly caused by the increment of substrate concentration around the enzyme due to the substrate adsorbing on the support. It could be another explanation for why those parameters were changed after enzyme immobilization in addition to some existing proposed reasons such as stabilizing conformation, facilitating dispersion of enzyme, etc.
研究领域:
生物技术
·
酶学
·
蛋白质工程
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DOI
高影响因子
Insects
·
2025
·Vol. 17
·期 1
·3
·
IF 2.9
Zhengyan Wang,Qiong Luo,Yifan Liu,Tianwei Ye,Yujia Zhang,Wei Xu
In agricultural production, the extensive use of insecticides has led to the persistent presence of residues such as deltamethrin in the environment, posing threats to ecosystems and human health. Utilizing microbes for environmental bioremediation presents a feasible approach. Studies have found that insects with strong insecticide tolerance often harbor gut bacteria capable of degrading insecticides. These gut bacteria are becoming an excellent reservoir of insecticide-degrading bacteria with bioremediation potential. However, in practical applications, the activity of free bacterial cells is easily affected by environmental factors, limiting the stability of their remediation efficacy. This study focused on a Microbacterium sp. strain isolated from insects. By optimizing culture conditions and the sodium alginate immobilization process, the remediation efficiency of the immobilized strain in contaminated soil and water was investigated. The results demonstrated that the immobilized strain significantly outperformed free bacteria in deltamethrin degradation performance in both contaminated soil and water, while also exhibiting good stability and reusability. This research provides an effective method for the remediation of deltamethrin-contaminated environments. Extensive application of deltamethrin on agricultural products results in serious contamination of the environment. Its negative impact on environmental and public health necessitates the development of environmental remediation technologies. Detailed investigations of microbial degradation of deltamethrin may be useful for the development of bioremediation strategies. In this study, the deltamethrin removal capability of a bacterial strain, Microbacterium sp., previously isolated from the gut of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) was first investigated. When 3 mL of the bacterial solution (OD 600 = 1) was inoculated into 97 mL of MS media containing 200 μg/mL deltamethrin, it could remove 45.7% deltamethrin after 64 h of incubation. This strain grew fastest in LB media with an inoculum volume of 3% in pH 7 at 175 rpm, 25 °C. To enhance its environmental tolerance, this strain was immobilized with sodium alginate. Microbacterium sp.-containing calcium alginate microspheres (CAMs) exhibited an enhanced deltamethrin removal capability compared to free bacteria, and CAMs generated by immobilization with 2% sodium alginate and 3% CaCl 2 cross-linking for 4 h possessed the maximum deltamethrin removal capability. The ultrastructure of Microbacterium sp.-containing CAMs prepared under optimal conditions was a three-dimensional mesh structure with pores and dense features, and the bacteria grew well in the immobilized carrier. After being reused five times, the deltamethrin removal rate of immobilized Microbacterium sp. still reached over 50%. When Microbacterium sp. was inoculated into deltamethrin-contaminated water or soil for 48 h, the deltamethrin removal rate of immobilized bacteria was 1.4 times higher than that of free bacteria. These results suggest that Microbacterium sp.-containing CAMs possess an excellent deltamethrin removal capability and good reusability, showing great potential for the remediation of deltamethrin-contaminated environments.
研究领域:
环境微生物学
·
生物修复技术
·
应用微生物学
·
农药降解
·
微生物发酵
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产品关联
DOI
PubMed
高影响因子
International Journal of Applied Glass Science
·
2025
·Vol. 17
·期 1
·e70012
·
IF 2.5
Mei Li,Zhaobo Li,Rugeng Liu,Meng Zhang,Wei Han
The proper disposal of nuclear waste is crucial to reducing the risks brought by the development of nuclear energy. In this study, zirconolite-based borosilicate glass–ceramics immobilized with praseodymium oxide (Pr 6 O 11 ) was studied and the influence of Pr 6 O 11 content on their stability was also investigated. The synthesis of zirconolite at 1300°C exhibited excellent crystallinity and morphology when doping different amounts of Pr 6 O 11 . A mixture of barium borosilicate glass and different amounts of ceramic raw materials was sintered at 1300°C. X-ray diffraction results showed that as the ceramic content increased, zirconolite has more crystallinity. The energy dispersive spectroscopy mapping of the glass–ceramics revealed an even distribution of praseodymium across the surface. By conducting chemical durability testing, the leaching rate of praseodymium (NR Pr ) in glass–ceramics was 2.208 ± 0.012 × 10 −6 g·m −2 ·day −1 at day 28. The results of the chemical durability tests indicate that zirconolite-based borosilicate glass–ceramics exhibit good leaching performance. This strong leaching performance is essential for the solidification of radioactive nuclides, ultimately facilitating the safe disposal of nuclear waste.
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 10.652
Wei Zhuang,Xuebo Quan,Zhenfu Wang,Wenfeng Zhou,Pengpeng Yang,Lei Ge,Byron Villacorta Hernandez,Jinglan Wu,Ming Li,Jian Zhou,Chenjie Zhu,Hanjie Ying
Compared to the traditional chemical reduction method, a green and efficient strategy was successfully applied to regulate the surface heterogeneity of graphene oxide (GO). In this work, different kinds of amino acids were used to reduce and modify GO. The reduction and adsorption disparities of amino acids contributed to the difference of structural heterogeneity in terms of density, thermal tolerance and disorder of the oxygen-containing groups of GO. The modification also resulted in discrepancies in surface properties of the GO samples, such as hydrophobicity and surface charge. The functionalized GO was employed as a carrier for the immobilization of lipase from Thermomyces lanuginosus (lipase TL). The storage stability, the temperature and pH sensitivity of the immobilized biocatalysts and the free lipase TL were studied. Lipase immobilized on functionalized GO demonstrates superior enzymatic loading and relative activity compared to GO, with a maximum increase of 70% and 47%, respectively. The relative activity of lipase immobilized on functionalized GO was 19.21 times more than that of commercial immobilized lipase TLIM. Through molecular dynamics simulations we concluded that the increase in the relative activity of lipase TL is mainly related to its adsorption orientation and slight conformational change. Thus, our research offered a green and effective strategy to regulate the heterogeneity of nanomaterials for a suitable microenvironment to enhance the performance of immobilized lipase.
研究领域:
生物化学
·
材料科学
·
酶工程
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DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2020
·
IF 3.644
Zang Yuan-Yuan,Yang Sha,Xu Yong-Qiang,Chen Zhi-Gang,Wu Tao
In this study, a strain with rutin degrading enzyme (RDE) activity was screened from moldy tartary buckwheat and subsequently identified as Fusarium spp. The structure and enzyme characteristics of CLEA-RDE formed by immobilization via cross-linking were then investigated. Further, the optimal catalysis conditions of CLEA-RDE in natural deep eutectic solvents (NADESs) serving as hydrolysis solvents were also investigated. The results of SEM and spectrum indicated that CLEA-RDE became more stable than free-RDE due to the cross-linking. Interestingly, CLEA-RDE showed a wider range of pH adaptation and higher tolerance to low temperatures (20 – 30°C) and hydrophobic environments. The results of orthogonal experiments revealed that the optimal condition for rutin hydrolysis was under pH 5.0 and 40oC with the degradation rate of 10.65 mg min−1 L−1. The preparation of CLEA-RDE without a carrier-based immobilization method reduces the loss of enzyme activity, improves the stability of the enzyme and can be applied to the investigation of immobilization of various enzymes, thus providing a referred idea for the improvement of catalysts in industrial production.
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DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 107
·88456-88463
·
IF 3.84
Anqiang Zhang,Liujun Cheng,Shuanghao Hong,Caixia Yang,Yaling Lin
Polydimethylsiloxane (PDMS) is a widely used material for biomedical applications. In this work, a convenient method for the covalent modification of PDMS with carboxybetaine was developed and used to construct a biocompatible and anti-fouling coating. Following the preparation of a Si–H functionalized PDMS film by adjusting the molar ratio of the two components used in Sylgard 184 silicone elastomer networks, the allyl carboxybetaine (ACB) was grafted to the PDMS surface via a hydrosilylation reaction in the presence of a Karstedt's catalyst. ATR-FTIR and water contact angle measurements revealed that carboxybetaine was introduced to the PDMS surface successfully. The biocompatibilities of PDMS and carboxybetaine-modified PDMS (PDMS-CB) films were evaluated by cytotoxicity, hemocompatibility, and dynamic clotting time. The anti-fouling properties of PDMS-CB were evaluated by protein adsorption and bacterial adhesion measurements. The results showed that the carboxybetaine layer could enhance the biocompatibility of PDMS and reduce the adsorption of protein and adhesion of bacteria efficiently.
研究领域:
生物材料
·
医学工程
✓ 已验证
DOI
高影响因子
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2017
·Vol. 17
·期 1
·370-376
·
IF 1.483
Xia Guo-Hua,Liu Wei,Jiang Xiao-Ping,Wang Xiang-Yu,Zhang Ye-Wang,Guo Jianyong
Magnetic Fe3O4 nanoparticles were prepared through hydrothermal method and coated with silica on the surface to obtain Fe3O4@SiO2 core–shell nanoparticles. After modification with different functional groups including aldehyde,
amine and diimide, the nanoparticles were used as carrier for covalent immobilization of lipase. The nanoparticles with aldehyde groups showed highest immobilization yield (52.8%) and efficiency (86.5%). And the immobilization conditions including pH, temperature and the concentration of enzyme
were optimized. After immobilization, the K
m of lipase was altered from 2.3 to 3.2 mM. The thermal stability and pH stability were enhanced by immobilization at the investigated conditions: pH 5.0–8.0 and temperature 30–70 °C. After 10 batches conversion of
4-Nitrophenyl palmitate into p-Nitrophenol, the immobilized lipase retained over 75% of the original activity. Compared with the commercial lipase Novozym435, the immobilized lipase showed better stability and higher catalytic efficiency. These results demonstrate that the immobilized lipase
on the modified Fe3O4@SiO2 magnetic nanoparticles has enhanced stability and reusability, which make lipase of potential interest in a number of industrial applications.
研究领域:
生物化学
·
纳米材料科学
·
酶工程
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF POROUS MATERIALS
·
2019
·Vol. 27
·期 2
·549-554
·
IF 1.947
Chen Meng,Wu Haoming,Li Zhentao,Wu Keke,Jiao Yanpeng,Zhou Changren
Chitin/graphene oxide (Ch/GO) composite aerogel beads were fabricated by a simple and environmentally friendly method for lipase immobilization. The morphology and specific surface area of these beads were investigated by scanning electron microscopy (SEM) and Brunauer–Emmett–Teller (BET), respectively. The results revealed that the prepared beads showed three-dimensional porous structure, and GO was successfully bound to chitin with enhanced surface area and mechanical strength. Candida rugose lipase (CRL) was immobilized on Ch/GO aerogel beads under optimum conditions by adsorption and subsequent cross-linking with glutaraldehyde which is added in the presence of the unbound proteins by conjugation. The maximum immobilization capacity of Ch, Ch/GO-5, Ch/GO-10 were 129.9, 141.1, 151.9 mg/g support . The immobilized lipases exhibited good temperature and thermal stability in comparison to free CRL. After recycling 5 times, the immobilized lipases remained more than 90% of their initial activity and the activity of Ch/GO-10 could remain 84.2% of free CRL. With these desired characteristics, the design of Ch/GO composite aerogel beads for lipase immobilization may have potential applications in various lipase-based industrial processes.
研究领域:
生物化学
·
材料科学
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DOI
高影响因子
JOURNAL OF SOLID STATE CHEMISTRY
·
2021
·
IF 3.498
Yaxin Feng,Guilin Wei,Yi Liu,Wenhong Han,Yan Chen,Runjie Sun,Lin Peng,Meihua Ma,Yu Zhang,Zhentao Zhang,Xirui Lu
The immobilization of iodine waste is a challenge in the field of nuclear waste . In this work, iodine waste (silver-coated silica gel with different iodine loadings) was immobilized at low-sintering temperatures (500, 550, and 600 °C). XRD represented that the crystallized phases of the sintered matrix consist of AgI and B 2 O 3 . The crystallization behavior of boron can be found as the sintering temperature increased from 500 °C to 600 °C. FT-IR results proved that in the process of crystallization, part of the microstructure related to B–O stretching transformed from [BO 4 ] to [BO 3 ] units, which benefits low-temperature iodine waste immobilization due to the bonding energy of [BO 3 ] is higher than that of [BO 4 ]. Our work reveals the B-related microstructure transition in low-temperature immobilization iodine waste.
研究领域:
核废物处理
·
材料科学
·
无机化学
·
放射化学
·
环境工程
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Jing Zhou,Dapeng Li,Shutong Chen,Yao Xu,Xue Geng,Chaoran Guo,Yong Huang
The immobilization of sedimentary phosphorus (P) by capping sediments with amended calcium peroxide material (ACPM) and CaO 2 was investigated, the sediments and overlying water from a black-odor river. Under the capping with ACPM and CaO 2 , the pH and dissolved oxygen (DO) in the overlying water were higher than in the control. This contributed to the more loss of external P under the ACPM (the phosphate sorption was fitted well by the Langmuir equation, the maximum P sorption capacity up to 29.28 mg P g −1 ). Additionally, ACPM improves the decrease of the reduction materials (e.g., NH 4 + -N, Fe 2+ ) in the pore water compared to that associated with CaO 2 , resulting in a less obvious dissolved inorganic P (DIP) in the pore water, but the accumulation of DIP in the pore water with CaO 2 suggested an explosive release once the micro-environment conditions encounters adverse conditions. The sharp reduction of Fe/Al-P and Res-P contributes to the increase of DIP in the pore water, with a release of up to 390.60 mg P kg −1 DW (dry weight), whereas the value is only 62.55 mg P kg −1 DW when capped with ACPM. Correspondingly, the percentage of the bioavailable P to Tot-P decreases rapidly from 17.27% (control, 0–2 cm) to 7.21% (ACPM, 0–2 cm) and 10.57% (CaO 2 , 0–2 cm), indicating the obvious P immobilization. The implication is that the application of CaO 2 is not suitable for direct use, while the combination with other materials (e.g., WPS) is a better choice.
研究领域:
环境科学
·
水体修复
·
环境化学
·
污染控制
·
沉积物管理
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DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2019
·
IF 3.218
Dou Ying,Xing Yan Xu,Chuan Bao Cao,Zhuo Chen
Although lithium sulfur batteries (LSBs) possess extremely high specific capacity and ultrahigh energy density , the low conductivity and severe shuttle effect greatly restrict its practical application. Herein, we report a facile strategy to overcome the problems via a double-layer structure, in which one provides strong chemisorption by functionalized reduced graphene oxide (rGO) while the other confines polysulfides physically through coating a layer of rGO film on the electrode. The strategy not only alleviates the dissolution and diffusion of polysulfides but also inhibits polysulfides from penetrating into anode to some extent and enhances the electrical conductivity of electrode. The LSBs employing double-layer structure electrodes exhibit high initial capacity of 1353 mAh g −1 at 0.1 C and 78% capacity retention after 100 cycles, good cycling stability with only 0.056% capacity decay per cycle at 1 C, and remarkable rate capability with 92% reversible capacity retention. The current findings manifest that the double-layer structure can effectively improve the electrochemical performance via the dual synergistic effect of both chemical interaction and physical confinement.
研究领域:
材料科学
·
储能
·
电化学
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DOI
高影响因子
危险品化学品经营许可证（带存储）