ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Jing Lei,Ling Zhang,Mei Li,Wei Liu,Yan Jin,Baoxin Li
Oxygen vacancy is one intrinsic defect in metal oxide materials. Interestingly, we herein found that the surface oxygen vacancy can significantly enhance the catalytic activity of Co3O4 nanowires in the luminol–H2O2 chemiluminescence (CL) reaction. 0.1 ng/mL Co3O4 nanowires containing 51.3% surface oxygen vacancies possessed ca. 2.5-fold catalytic activity of free Co2+ (the best metal ionic catalyst for the luminol–H2O2 CL reaction). The superior catalytic efficiency is attributed to the enhanced adsorption of H2O2 by surface oxygen vacancies, which in turn accelerates the cleavage of O–O bonds and generates •OH radicals. More importantly, the surface oxygen vacancy-rich Co3O4 nanowires retained about 90% catalytic activity after modification with antibodies. The surface oxygen vacancy-rich Co3O4 nanowires were used to label the secondary antibody, and one sandwich-type CL immunoassay of carcinoembryonic antigen was established. The detection limit was 0.3 ng/mL with a linear range of 1–10 ng/mL. This proof-of-concept work proves that surface oxygen vacancy-rich Co3O4 nanowires are suitable for labeling biomolecules in CL bioanalysis and biosensing.
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高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Xinyi Wang,Jing Wang,Houcheng Ding,Yongping Dong
Covalent organic framework (COF) materials exhibit potential application in electrochemical sensing and attract increasing research interests. In this study, a novel signal-off mode electrogenerated chemiluminescence (ECL) sensor based on gold nanoparticle decorated COF (Au@COF) composite was developed. The oxidation of luminol was promoted at ferrocene (Fc) modified electrode to generate strong anodic ECL signal. The resonance energy transfer (RET) between luminol ECL and Au@COF composite could inhibit ECL emission. The acetylcholinesterase (AChE) aptamer connected Au@COF composite was immobilized onto the electrode through DNA hybridization to construct ECL-RET system. The specific recognition between AChE and its aptamer could release Au@COF composite from the electrode surface to block the ECL-RET process and restore the ECL signal. As a result, an ‘‘off-on’’ type ECL sensor was constructed and used to detect AChE in the range of 0.5 nM-1 μM, with a detection limit of 1.7 × 10 -10 mol/L (3σ). The sensor has high sensitivity and good stability, indicating that COF composite has attractive potential application in ECL sensing field.
研究领域:
生物传感
·
分析化学
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高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 9
·1-11
·
IF 5.3
Lu Juan,Guo Yanjia,Shan Xiangyu,Song Yujia,Li Ruidan,Tian Li,Li Xuwen
A solid-state electrochemiluminescence (ECL) sensor was fabricated by immobilizing luminol, a classical luminescent reagent, on a Zn-Co-ZIF carbon fiber-modified electrode for the rapid and sensitive detection of procymidone (PCM) in vegetable samples. The sensor was created by sequentially modifying the glassy carbon electrode with Zn-Co-ZIF carbon fiber (Zn-Co-ZIF CNFs), Pt@Au NPs, and luminol. Zn-Co-ZIF CNFs, prepared through electrospinning and high-temperature pyrolysis, possessed a large specific surface area and porosity, making it suitable as carrier and electron transfer accelerator in the system. Pt@Au NPs demonstrated excellent catalytic activity, effectively enhancing the generation of active substances. The ECL signal was significantly amplified by the combination of Zn-Co-ZIF CNFs and Pt@Au NPs, which can subsequently be diminished by procymidone. The ECL intensity decreased proportionally with the addition of procymidone, displaying a linear relationship within the concentration range 1.0 × 10 –13 to 1.0 × 10 –6 mol L −1 (R 2 = 0.993). The sensor exhibited a detection limit of 3.3 × 10 –14 mol L −1 (S/N = 3) and demonstrated outstanding reproducibility and stability, making it well-suited for the detection of procymidone in vegetable samples. Graphical Abstract
研究领域:
分析化学
·
食品安全检测
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高影响因子
LUMINESCENCE
·
2026
·Vol. 41
·期 7
·e70567
·
IF 2.8
Fenqiang Luo,Liyan Zheng
Novel multifunctional metal–organic frameworks prepared by encapsulating luminol, hemin, and glucose oxidase within UiO-66 have been employed for the chemiluminescent determination of glucose via a one-step method. Benefitting from the encapsulating cascade catalyst with luminescent reagent in UiO-66, the as-prepared material can be utilized to fabricate practical glucose sensors with a wide response range, a low detection limit, and a short detection time. This promising sensing material is anticipated to have widespread applications in biotechnology and demonstrates significant potential for the green, selective, and sensitive detection of target analytes.
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高影响因子
MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 5
·348
·
IF 5.4
Wang Jing,Fang Zihan,Wang Xinyi,Dong Yongping,Ding Houcheng
Two covalent organic framework (COF) materials—TAPA-TFPB-COF and TAPA-BTT-COF—were synthesized through Schiff-base condensation reactions, and their catalytic performance in the luminol-H 2 O 2 cathodic electrochemiluminescence (ECL) system was systematically investigated. The results demonstrated that TAPA-BTT-COF exhibited superior peroxidase-like activity, which could effectively catalyze the decomposition of H 2 O 2 to generate reactive oxygen species (ROS) and significantly enhance the ECL intensity. Based on this catalytic mechanism, a highly sensitive and selective method for the detection of tyrosine (Tyr) was established, with a linear detection range from 1.0 × 10 − 8 to 1.0 × 10 − 4 mol L − 1 and a detection limit as low as 6.2 × 10 − 10 mol L − 1 . This study innovatively explored and utilized the intrinsic enzyme-like activity of COF materials as a substitute for traditional biological enzymes to construct a stable, cost-effective, and ultrasensitive enzyme-free ECL sensing platform, which might further expand the biosensing application of COF materials.
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高影响因子
RSC Advances
·
2026
·Vol. 16
·期 30
·27756-27764
·
IF 6.1
Chunchun Wei,Mixia Chen,Wei Hu,Fei Yan,Yu Cui
An enzymatic electrochemiluminescence (ECL) sensor based on a ternary luminol system is developed for highly sensitive detection of uric acid (UA). The platform integrates nanochannel-confined platinum nanoparticle (PtNPs) and nitrogen-doped graphene quantum dot (NGQDs) nanocomposites (PtNPs–NGQDs) as coreaction accelerators, with urate oxidase (Uox) covalently immobilized at the nanochannel entrances. In this design, an amino-functionalized vertically ordered mesoporous silica film (NH2-VMSF) confined the PtNPs–NGQDs within its nanochannels, while Uox is attached to the outer surface. The confined PtNPs–NGQDs exhibit a synergistic catalytic effect, amplifying the ECL signal of the luminol–H2O2 system by 16.1-fold. In the presence of UA, immobilized Uox catalyzes its oxidation to generate H2O2, which is subsequently decomposed by the PtNPs–NGQDs nanocomposite, leading to enhanced ECL emission. Under optimized conditions, the sensor exhibits two linear response ranges for UA (0.01–1 µM and 1–50 µM) and a low detection limit of 8.1 nM (S/N = 3). The sensor also demonstrates good selectivity and stability, enabling reliable UA quantification in complex urine samples. This work provides a simple and versatile strategy for constructing sensitive ECL enzymatic platforms suitable for the detection of metabolites.
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高影响因子
BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Hang Ao,Jinshan Xiong,Wencheng Xiao,Wenrui Hu,Jie Wu,Huangxian Ju
Owing to the continuous mutations of the coronavirus, the sensitive and accurate detection of the SARS-CoV-2 nucleocapsid protein is still necessary in clinical diagnosis. Aim to the challenge of sensitivity in chemiluminescence (CL) imaging detection and the need for high-throughput clinical screening, this work used imidazole (Im) as an enhancer for hemin-DNA/luminol/H 2 O 2 CL system to propose a CL imaging method for SARS-CoV-2 nucleocapsid protein detection. In this system Im could reduce the hemin-DNA catalyzed oxidation energy of luminol by H 2 O 2 to promote the CL emission through the accelerated breaking of O-O bond of dianionic cyclic peroxide. The enhancement mechanism was demonstrated with theoretical calculations and several characterization techniques. By combining the proximity-induced initiator release with immunological recognition, an initiator-triggered DNA nanomachine was designed to achieve the release of abundant primers for activating hemin-DNA switch. This signal amplification strategy led to strong CL emission for highly sensitive imaging detection of the target. The Im-enhanced hemin-DNA/luminol/H 2 O 2 CL imaging assay exhibited a linear detection range over 4 orders of magnitude with a detection limit down to 2.75 pg/mL for SARS-CoV-2 nucleocapsid protein, exhibiting promising potential of both Im sensitization and the proposed DNA nanomachine to improve the sensitivity of high-throughput CL biosensing for the early diagnosis and clinical screening of the coronavirus and other diseases.
研究领域:
生物分析化学
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生物传感器
·
临床诊断
·
病毒学
·
纳米生物技术
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高影响因子
ELECTROCHIMICA ACTA
·
2014
·
IF 4.086
Zhiyong Guo,Shuping Du,Beibei Chen,Yuhong Sha,Bin Qiu,Xiaohua Jiang,Sui Wang,Xing Li
An ultrasensitive sandwich-type label-free electrochemiluminescence (ECL) immunosensor based on sombrero model for the detection of neurotensin (NT) in human serum and urine was developed. Graphene-hyaluronate-luminol (G-HY-luminol) composite was coated on the surface of the glassy carbon electrode to build the ECL immunosensor, which gave in-situ ECL signals by luminol. A secondary NT unlabelled antibody was used to form the final sandwich immunocomplex as a sombrero model, facilitating reduction of the ECL intensity to improve the sensitivity of detection significantly. Results showed that the inhibition of ECL intensity increased with the logarithm of NT concentration within a wide linear range from 0.001 pg cm −3 to 100 pg cm −3 . In addition, specificity, stability, reproducibility, regeneration and application were satisfactory. Therefore, this developed ECL immunosensor has a potential for practical detection of NT and NT-like peptides with small molecular weight in the clinical diagnostics.
研究领域:
生物医学工程
·
分析化学
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2011
·
IF 1.77
Wenwen Liu,Wei Cao,Weihua Liu,Kang Du,Pixue Gong
This study established a novel flow injection (FI) methodology for the determination of phenol in aqueous samples based on luminol chemiluminescence (CL) detection. The method was based on the inhibition that phenol caused on the hemin-catalysed chemiluminescence reaction between luminol and hydrogen peroxide in alkaline solution. Optimum conditions and possible mechanisms have been investigated. The linear range was 2.0 × 10 −9 to 4.0 × 10 −7 g mL −1 for phenol. The proposed method is sensitive with a detection limit of 4.0 × 10 −10 g mL −1 . The relative standard deviation for 11 measurements was 2.3% for 1.0 × 10 −7 g mL −1 phenol. The method was applied for the determination of phenol in waste water samples. The results obtained compared well with those by an official method.
研究领域:
分析化学
·
环境科学
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高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 8
·1-7
·
IF 5.705
Wu Yue,Wang Mengyao,Ouyang Hui,He Yong,Zhao Xiaoyan,Fu Zhifeng
An affinity-based protocol is described for the detection of Staphylococcus aureus ( S. aureus ). It is utilizing teicoplanin-functionalized magnetic beads as carriers. Teicoplanin, which binds to the walls of cells of S. aureus via five hydrogen bonds, acts as the recognition agent. Captured S. aureus is magnetically separated from the sample matrix and then specifically lysed by lysostaphin which cleaves the cross-linking pentaglycine bridges of peptidoglycan in the cell wall. Lastly, S. aureus is quantified via the inhibitory effect of released intracellular catalase on a chemiluminescent (CL) system composed of peroxidase, luminol, H 2 O 2 and p -iodophenol because catalase decomposes H 2 O 2 . S. aureus can be detected with CL response in the 140 to 1.4 × 10 7 CFU·mL −1 concentration range and a detection limit as low as 47 CFU·mL −1 at a signal-to-noise ratio of 3. The method was evaluated by analyzing spiked samples including milk, human urine and saline injection solutions. The reliability was demonstrated by a recovery test and by comparison with a conventional plate counting method.
An antibiotic-affinity protocol is developed to detect Staphylococcus aureus ( S. aureus ) by utilizing teicoplanin-functionalized magnetic beads (Teic-MBs) as carriers. S. aureus can be quantified by measuring the inhibition of luminol chemiluminescence (CL) signal by intracellular catalase.
研究领域:
微生物学
·
分析化学
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Shuting Cheng,Huimin Liu,Hui Zhang,Guanglei Chu,Yemin Guo,Xia Sun
An ultrasensitive electrochemiluminescence (ECL) aptasensor for detection of kanamycin (KAN) was constructed by using the chemiluminescence properties of luminol-hydrogen peroxide (H 2 O 2 ) in alkaline solution. Silver nanoparticles (AgNPs) could act as effective catalyzer to accelerate H 2 O 2 decomposition to produce various intermediate reactive oxygen species for signal amplification and thus increased the ECL intensity of luminol. When the target KAN was in the presence, ECL signal decreased obviously owing to the specific interaction between KAN and aptamer. Thus, the concentration of KAN could be determined according to the intensity of ECL. To further demonstrate the effect of aptasensor, we had conducted cyclic voltammetry on an electrochemical workstation and electrogenerated chemiluminescence analyzer, respectively. Upon the optimization of assay conditions, the proposed aptasensor displayed a wide linear range from 0.5 to 100 ng mL −1 with limit of detection of 0.06 ng mL −1 . The as-proposed aptasensor was not only capable of achieving a satisfactorily low detection limit but also possessed advantages of excellent accuracy, specificity and stability. Furthermore, this constructed ECL sensing platform was successfully applied to detect KAN in real samples and it would be of excellent potential to detect other antibiotics using the proposed method.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
环境科学
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高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Yu Du,Siqi Yu,Huangxian Ju
A quenching-typed electrochemiluminescence (ECL) immunosensing method was proposed for protein detection by the redox reaction of tungsten-based polyoxometalate nanoclusters (W-POM NCs) with free radical (O 2 •– ) as the intermediate of co-reactant H 2 O 2 . The immunosensor was constructed by co-immobilizing luminol loaded CeVO 4 /Au nanohybrids and capture antibody on an indium tin oxide electrode, which showed strong ECL emission in the presence of H 2 O 2 due to the catalysis of CeVO 4 /Au toward its electro-oxidation to produce O 2 •– via the reversible conversion between Ce 3+ and Ce 4+ . The W-POM NCs were encapsulated in the uniformly sized amino-silica nanosphere (SiO 2 ) to act as a label ( [email protected] 2 ) of the secondary antibody. Upon the sandwich-typed immunoreactions with the target, the W-POM NCs were introduced onto the immunosensor surface to reduce O 2 •– , and thus quenched the ECL emission of luminol-H 2 O 2 system. Using neuron-specific enolase (NSE) as a target, the proposed method showed a detection limit of 0.19 pg/mL and detectable range of 0.5 pg/mL to 18 ng/mL. The excellent performance of the proposed “ON-OFF” strategy demonstrated that the W-based quenching offers an effective tag for immunoassay of protein biomarkers.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
免疫学
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高影响因子
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