Materials Today Communications
·
2021
·
IF 3.383
Ruizhe Lian,Jinxing Cao,Xiaohong Jiang,Aleksandr V. Rogachev
Chitosan/gelatin/poly (vinyl alcohol) (CS/Gel/PVA) composite films prepared by the tape-casting, with ciprofloxacin hydrochloride (CIP) as model drug incorporated, can prolong the drug release cycle. The results show that the diameter of CS/Gel/PVA/CIP composite films against Staphylococcus aureus could reach 15 mm after 30 days of sustained release in phosphate buffered solution. Moreover, the CIP release is divided into the rapid release stage and slow-release stage, which achieves rapid sterilization and long-term antibacterial effect. The results show that after 30 days of immersion in PBS, the CIP cumulative release concentration from CS/Gel/PVA/CIP composite films is 13.53 μg/mL, and the CIP cumulative release concentration reaches 87.8 % of the total release on the 3th day of immersion. Meanwhile, the thermal stability and mechanical properties of CS/Gel/PVA/CIP composite film can be improved when the CIP is added into CS/Gel/PVA composite films. Besides, the cell viability of CS/Gel/PVA/CIP composite films reaches 90.85 ± 5.50 % ( p < 0.05) after co-culture with MC3T3-E1 cells for 48 h, which proves that CS/Gel/PVA/CIP composite films have good cytocompatibility and biosecurity.
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Zhengwei Liu,Xiaoqiong Mo,Fenbo Ma,Sijing Li,Guofeng Wu,Bin Tang,Lijun Lin
Osteoarthritis (OA) is an age-related joint disorder and one of the leading causes of physical disability. In this study, we designed and synthesized a new polysaccharide complex, carboxymethyl chitosan strontium (CMCS-Sr), which is believed to have positive effects on relieving OA. The synthesized CMCS-Sr was structurally verified by SEM, EDS, FTIR, etc. The therapeutic effects of CMCS-Sr were evaluated using various biological experiments. The cell viability and apoptosis results reveal that CMCS-Sr can significantly promote the proliferation and suppress OA chondrocytes apoptosis in vitro . The immunofluorescence staining results suggest that CMCS-Sr facilitates the promotion of the secretion of Type II collagen (Col-II). The transcriptomic results support the observed positive effects of CMCS-Sr on inhibiting chondrocytes apoptosis and alleviating inflammatory reactions. Moreover, animal study demonstrates that CMCS-Sr effectively reduced articular cartilage damage and subchondral bone degradation. Therefore, we propose the use of CMCS-Sr as a promising candidate for relieving OA.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MATERIALS CHEMISTRY AND PHYSICS
·
2021
·
IF 4.094
Qiuhua Yuan,Ziqiang Zhang,Yuan Yang,Youliang Jian,Ruilong Li,Xiaoyi Dai,Wenshan Wu,Junxi Zhong,Chen Chen
Hydroxyapatite (HA) is widely used in bone repair and regeneration because of its composition and structure similar to human bone. However, the artificially synthesized HA has poor mechanical properties , so it is often necessary to add a second phase to the powder as a supporting phase to enhance its mechanical properties. Strontium (Sr) has the properties of promoting the growth of bone cells, which can increase bone formation in the body and reduce bone self-absorption. Nevertheless, Sr-doped HA composites have been rarely reported in the literature in recent years. In this paper, Sr-doped HA was employed to be complexed with chitosan (CS) for the first time to prepare a composite coating that is dense and uniform, with good adhesion and ductility, in order to expand its clinical applications. Therefore, a series of HA samples doped with different Sr mole fraction (0 mol% (pure HA), 0.5 mol%, 1 mol%, 2 mol%, 4 mol%) were prepared by chemical precipitation method. Then the HA/CS composite coating doped with Sr was prepared on the stainless steel substrate by spin-coating technology. The results showed that Sr 2+ ions had replaced part of Ca 2+ ions and have been successfully doped into the HA lattice. The synthesized HA particles showed a shortrod-like morphology, and the substitution of Sr ions promoted the growth of Sr-doped HA crystals and increased its grain size . In addition, the synthesized Sr-doped HA/CS composite coating is dense and uniform. In vitro cell culture experiments showed that all composite coatings have good biocompatibility. The HA/CS coating with 4 mol% Sr ion doping is more conducive to cell proliferation , adhesion and osteogenic differentiation.
✓ 已验证
产品关联
DOI
高影响因子
COMPOSITES PART B-ENGINEERING
·
2021
·
IF 9.078
Riwang Li,Yuhui Zhang,Zhen Lin,Qiqi Lei,Yi Liu,Xinyang Li,Mingxian Liu,Gang Wu,Simin Luo,Huajun Wang,Xiaofei Zheng,Lihua Li,Ningjian Ao,Zhengang Zha
Breast cancer is one of the frequently occurring malignant tumors in the world. Breast resection, high tumor recurrence rate and high dose chemotherapy cause great pain to patients. Is there a way to prevent tumor recurrence efficiently while promoting new tissue generation after tumor resection? Based on this, an injectable biocompatible thiolated chitosan (CSSH) hydrogel with pH-response was designed to be used for accurate treatment of tumor cells in the initial stage of tissue resection and for tissue reconstruction later. To improve the mechanical strength of hydrogels, reduce the toxic and side effects, and delay the release of doxorubicin (DOX), halloysite nanotubes (HNTs) were modified to obtain thiolated HNTs (HNTs-SH), then loaded DOX ( [email protected] ), and further cross-linked CSSH to form [email protected] /HNTs-SH Gel. The results showed that HNTs-SH can be evenly dispersed in the gel matrix, which improve the compressive strength of the hydrogels. In vitro DOX release experiments showed that the hydrogels were pH sensitive, DOX released slowly at normal physiological pH, but released quickly in the acidic microenvironment of tumors. Cell experiments showed that [email protected] /HNTs-SH Gel released DOX can be taken up by MCF-7, thereby effectively inhibited its growth. In vivo recurrence experiments showed that the carriers can reduce the toxic and side effects of DOX, and effectively inhibited the recurrence and repair the defect tissue after tumor resection. Therefore, the drug-loaded gel is expected to be used as a carrier for local accurate and effective drug release, especially for inhibiting recurrence and repairing defective tissues after tumor resection.
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE RESEARCH
·
2021
·
IF 2.104
Lin Yue,Meihong Zheng,Min Wang,Imran Mahmood Khan,Bin Wang,Xiaoyuan Ma,Chifang Peng,Zhouping Wang,Wenshui Xia
The objectives of the present study are to synthesize a series of chitosan oligosaccharide-O-Terpenol (COS–O-Ter) derivatives and their implication to evaluate in vitro antibacterial activity . Herein, a general strategy is described for preparing COS–O-Ter derivatives, including substitution and deprotection reactions . The structures of COS–O-Ter derivatives were characterized by FT-IR, 1 H NMR, XRD, TGA , and elemental analysis . COS–O-Ter derivatives revealed the excellent solubility and in vitro antibacterial activity. Moreover, their antibacterial activities were more sensitive to Staphylococcus aureus ( S. aureus ) than Escherichia coli ( E. coli ) indicating the effective potential application of COS–O-Ter derivatives as natural antibacterial agents . The aforementioned study opens a pave to expand the application scope of COS and its derivatives in the food and pharmaceutical industries .
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Carbohydrate Polymer Technologies and Applications
·
2020
·
IF 0
Wentao Huang,Liping Zhu,Dezhong Liu,Jiefu Li,Shuguang Yang
Alginate (ALG) and chitosan (CHI) are naturally occurring polymers, and present broad applications in various fields. Owing to the electrostatic interactions between the two polymers, ALG and CHI are combined to prepare fibers via diffusion controlled in-situ polyelectrolyte complexation. A map of the concentration combination of spinning and coagulation solution is presented for the fabrication of the complex fibers. When concentration combination meets the requirements for fiber formation, increasing the concentration of the spinning or coagulation solution will slow down the diffusion, which will affect the composition and mechanical properties of the prepared fibers. Prolonging complexation time or increasing temperature will enhance the diffusion, and more polyelectrolytes with opposite charged-groups will be taken from the coagulation solution, hence the fibers modulus will increase. In addition, the fiber composition and initial modulus are found to be correlated, and the inner structure of the fiber is revealed using the fluorescence imaging technique.
✓ 已验证
产品关联
DOI
RSC Advances
·
2020
·Vol. 10
·期 73
·45130-45138
·
IF 3.119
Li Li,Shasha Han,Sengqun Zhao,Xurui Li,Bingmi Liu,Yu Liu
Metal–organic frameworks (MOFs) are composed of both organic linkers and metallic ions, which have emerged as excellent drug delivery agents for the treatment of cancer and other diseases. Currently, MOF studies are mainly focused on intravenous administration, while studies dedicated to oral administration are relatively scarce. In this study, five MOFs, namely UiO-66, UiO-66-NH2, UiO-66-COOH, UiO-67 and Zr-NDC, were synthesized, of which Zr-NDC had the largest drug loading capacity for 5-FU. Next, a chitosan (CS) modified Zr-NDC was developed to provide a strong impetus for the oral administration of 5-FU. In vitro release experiments of fluorescein isothiocyanate (FITC)-labeled chitosan demonstrated that the cumulative release rates of FITC-labeled chitosan in artificial gastric juice and artificial intestinal fluid were about 20% and 90%, respectively. The in vitro drug release profiles showed that under the protection of CS-MOF, the release of 5-FU into an acidic environment was only 20%, but the release in artificial intestinal fluid reached 70%. Pharmacokinetic analysis revealed that the coating of chitosan on the surface of MOFs exerted a controlled drug release effect, and further improved the oral bioavailability of 5-FU. These findings suggest that CS coating can break through the limitation of MOF intolerance to acid. It is expected that CS-MOF@5-FU can serve as a potential drug delivery system for the oral administration of 5-FU.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2020
·
IF 4.389
Chenmeizi Wang,Ling Wang,Qingle Zhang,Lu Cheng,Haitao Yue,Xin Xia,Huimin Zhou
Addition of nanomaterials into polymer matrix is a promising avenue to reinforce the mechanical properties of composites. In this work, chitosan-based hydrogels reinforced by cellulose nanofibers (CNFs) have been prepared by chitosan as matrix. The effect of CNFs (1vt%, 1.5vt% and 2vt%) on the structure-property relationship in mechanical, swelling capability, pH sensitivity and antibacterial have been investigated, respectively. Different from normal reinforced particle, CNFs with special fiber shape and abundant hydrogen bonding can form interconnected porous structure with chitosan hydrogel, which can spread stress and defer permanent damage under compressing. And the compressive strength increases nearly 20 % and swelling capacity achieve 140 %, when 1.5vt% CNFs added. Thanks to CNFs which obtained from apocynum venetum generated positive effect on the antibacterial rate against E. coli and S. aureus of composite hydrogel. It proves that CNFs display an excellent mechanical and antibacterial enhancement in composite, and it provided a new prospect for the rational selection of the different shapes and aspect ratio of reinforced materials. The obtained CNFs reinforced composite hydrogels could be potentially applied in antibacterial biological and food packaging area.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2020
·Vol. 25
·期 17
·3812
·
IF 3.267
Jifeng Li,Ting Fang,Wenjing Yan,Fei Zhang,Yunhui Xu,Zhaofang Du
In this study, oxidized chitosan grafted cashmere fibers (OCGCFs) were obtained by crosslinking the oxidized chitosan onto cashmere fibers by amide covalent modification. A novel method was developed for the selective oxidation of the C6 primary hydroxyls into carboxyl groups for chitosan. The effect of oxidization reaction parameters of HNO3/H3PO4–NaNO2mediated oxidation system on the oxidation degree, structure, and properties of chitosan were investigated. The chemical structure of the oxidized chitosan was characterized by solid-state cross-polarization magic angle spinning carbon-13 Nuclear Magnetic Resonance (CP/MAS13C-NMR), Fourier transform infrared spectroscopy (FT-IR), and its morphology was investigated by scanning electron microscopy (SEM). Subsequently, the effect of the oxidized chitosan grafting on OCGCF was examined, and the physical properties, moisture regain, and antibacterial activity of OCGCFs were also evaluated. The results showed that oxidation of chitosan mostly occurred at the C6 primary hydroxyl groups. Moreover, an oxidized chitosan with 43.5–56.8% carboxyl content was realized by ranging the oxidation time from 30 to 180 min. The resulting OCGCF had a C–N amido bond, formed as a result of the reaction between the primary amines in the cashmere fibers and the carboxyl groups in the oxidized chitosan through the amide reaction. The OCGCF exhibited good moisture regain and remarkable bacteriostasis against bothStaphylococcus aureusandEscherichia colibacteria with its durability.Keywords:chitosan;cashmere fiber;oxidization;graft;antibacterial property
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
·
2020
·Vol. 146
·期 4
·1629-1639
·
IF 2.731
Song Qingyi,Wu Hongjuan,Liu Hao,Wang Tian,Meng Weihua,Qu Hongqiang
A series of chitosan-modified inorganic oxyacid salt flame retardants (IOS-CS) were synthesized and used as flame retardants to improve the fire resistance of poly(vinyl chloride) (PVC) composites while improving the mechanical properties of the materials. The thermal stability of the material was analyzed by TG test; the flame-retardant and smoke suppression properties were evaluated by limiting oxygen index (LOI) and cone calorimeter test, and the mechanical properties were analyzed by tensile test and impact test. The TG results showed that the addition of flame retardant can promote the early cross-linking decomposition of PVC composites, not only advance the decomposition temperature of the material but also promote the formation of char layer and increase the char residual content. LOI and cone calorimeter test results showed that stannate chitosan (Sn-CS) can improve flame retardant and smoke suppression more effectively than other samples. Compared with pure PVC, the addition of Sn-CS increased its LOI from 26.6 to 30.5% and reduced the total heat release value and total smoke release by 18.23% and 52.55%, respectively. At the same time, the addition of the flame retardants had no large deterioration effect on the mechanical properties of the material. The tensile properties of flame-retardant PVC were slightly reduced compared with pure PVC, and the impact resistance was even improved to some extent.
✓ 已验证
产品关联
DOI
高影响因子
POLYMER DEGRADATION AND STABILITY
·
2020
·
IF 4.032
Xianzhen Dong,Qiao Cheng,Yanpiao Long,Chao Xu,Honglin Fang,Yuzhe Chen,Honglian Dai
Chitosan exhibiting excellent functional properties was found to be potential in a wide variety of industrial and biomedical applications. The lack of biological activities and the poor mechanical properties somewhat limit its applications. To address these limitations, chemical modification has been proved to be an efficient method. Here, we immobilized arginine-glycine-aspartic acid (RGD) on chitosan molecules and incorporated the chitosan with Poly(L-lactide-co-glycolide)-Poly(ethylene glycol) (PLGA-PEG) and β-tricalcium phosphate (β-TCP) nanoparticles to obtain a chitosan-based scaffold with enhanced mechanical and biocompatible performance. XPS and FT-IR confirmed the successful grafting of RGD peptides onto the chitosan chain. The mechanical properties, hydrophilicity and degradation behaviors of the membrane were characterized by stress-strain, water contact angle, and weight loss, respectively. Cytotoxicity, cellular adhesion, and cellular viability of the scaffolds were studied by MTT assay, cell adhesion, and cell survival experiments, respectively. The advantageous mechanical properties and the hydrophilic surface of the chitosan-based scaffold were achieved by blending with PLGA-PEG. The preliminary biocompatibility studies revealed that all the scaffolds were cell compatible, and the hydrophilic surface was more suitable for cell adhesion. The scaffolds grafted with RGD exhibited high cell adhesion rate than the pure chitosan. The results suggested that the surface hydrophilicity and biocompatibility of the chitosan-based scaffolds play an important role in enhancing cell adhesion and growth.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Qian Mao,Min Li,Shengjie Zhang,Xiujuan Zhang,Gaohong He,Wenjun Zhang
Alginate or chitosan microparticles as drug loading system performed pH-responsiveness and biocompatibility, yet with the burst-release and limited encapsulation. In order to improve the performance, herein, Pickering emulsion of chitosan-hydrophobic alginate nanocomposite (HSA-CS NCs) as the bio-stabilizer, was proposed as the drug-loading vehicle. Integrating the merits of HSA-CS and Pickering emulsion, such drug carrier of emulsion performed pH-response and biocompatibility from HSA-CS, and high loading capacity and rigid layer from Pickering emulsion, so as for the manipulated release behavior. With thorough investigation, via the various pH-response of HSA-CS nanocomposite in the continuous simulated gastrointestinal fluid, Pickering emulsion gradually released the loading drug (ibuprofen) out, performing the pH-triggered controlled-release behavior. Ibuprofen-loaded Pickering emulsions (30 mg/mL) released nearly none in SGF for 3 h, whereas in SIF, performed constant release in initial 5 h and continuous-release of 88.37% ibuprofen in 24 h with no drug-burst and high loading capacity, promisingly as the pH-responsive vehicle for drug delivery in oral route.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）