Advanced Healthcare Materials
·
2025
·
IF 9.6
Chenglong Li,Shuhao Xu,Xin Li,Huaiyu Su,Can Qian,Yingying Hou,Sanjun Shi
Rheumatoid arthritis (RA) is a chronic autoimmune disorder characterized by joint inflammation, damage, and disability. Activated fibroblast-like synoviocytes (FLSs), abundant in RA synovium, crucially facilitate disease progression. These activated FLSs drive RA pathogenesis by upregulating adhesion molecules, proinflammatory cytokines, chemokines, and major histocompatibility complex class II (MHC-II). This upregulation facilitates their interaction with CD4 + T cells, triggering an inflammatory cascade that exacerbates synovial inflammation. To address this, a DSPE-mPEG 2000 -GPA-KLVFF-decorated liposome loaded with dexamethasone (DPGK@Lipo-Dex) is developed to precisely target FLSs in arthritic joints. DPGK@Lipo-Dex achieves efficient binding to FLSs through a specific enzymatic interaction between fibroblast activation protein-α (FAP-α) and GPA, along with prolonged retention in the joints due to the fibrillar transformation of KLVFF. In vitro studies demonstrate that DPGK@Lipo-Dex enhances cellular uptake and reduces MHC-II expression on aggressive FLSs. In adjuvant-induced arthritis (AIA) rats, DPGK@Lipo shows specific distribution patterns that target both inflamed joints and FLSs. Treatment with DPGK@Lipo-Dex leads to a downregulation of MHC-II expression, a decrease in the number of activated T cells within the synovium, and a reduction in levels of proinflammatory mediators. Overall, DPGK@Lipo-Dex effectively suppresses FLSs-related responses and ultimately results in inflammatory remission. This makes DPGK@Lipo-Dex a promising candidate for nano-therapeutic treatment in rheumatoid arthritis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yiling Chen,Yong Qin,Haixin Yi,Xiaoyu Cui,Xu Wang,Xiangyu Xu,Yongmei Chen,Fuchuan Li,Wenshuang Wang
Glucans, as versatile biomacromolecules, hold significant application potential across various industries. Volutharpa ampullacea perryi , an affordable deep-sea gastropod, demonstrates considerable commercial promise. In this study, a novel sulfated α-glucan (VpG) was isolated and identified from Volutharpa ampullacea perryi for the first time. Compared to other α-sulfated glucans, it has a high degree of sulfation (18.6 %) and an ultra-high molecular weight of approximately 2250 kDa. Further NMR analysis, including 1 H, 13 C, COSY, TOCSY, HSQC and HMBC, revealed that the anomeric signals at 5.35 ppm dominated. Therefore, VpG is mainly consisted of a repeating α-(1 → 4)-linked glucose backbone with β-(1 → 6) and β-(1 → 3)-linked glucosyl branches. Furthermore, bioactivity evaluation demonstrated that VpG significantly alleviates inflammation in dextran sulfate sodium-induced mouse models of ulcerative colitis, effectively inhibiting body weight loss by 20 % and colon shortening by 31.26 %. The strong anti-inflammatory activity of VpG indicates its potential in pharmaceutical development and provides a novel approach for the high-value utilization of Volutharpa ampullacea perryi .
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Microbiology Spectrum
·
2025
·
IF 3.8
Yuhan Yang,Panjie Hu,Yanchun Gong,Zeyong Zhong,Yichi Zhang,Fan Ye,Tieli Zhou,Jianming Cao,Zhongyong Wang
Multidrug-resistant gram-negative bacteria (GNB) have posed a serious public health hazard worldwide. Colistin (COL), the ultimate therapeutic option for treating GNB infections, has had escalating resistance rates concomitant with increased utilization. In this study, we attempted to increase the sensitivity to COL using a plant extract, α-terpineol (α-TP). Checkerboard and time-kill assays indicated that the combination of COL and α-TP exhibited pronounced synergistic antimicrobial effects against colistin-resistant (COL-R) GNB in vitro. Biofilm assessments revealed that COL combined with α-TP effectively inhibited biofilm development and eliminated established biofilms. Scanning electron microscopy indicated substantial disruption of bacterial cell membranes after 6 h of combined treatment. Additionally, cytotoxicity and erythrocyte hemolysis assays confirmed that α-TP maintained a degree of safety at synergistic concentrations. Mechanistically, the combination of COL and α-TP promoted the accumulation of reactive oxygen species and enhanced the permeability of both the inner and outer bacterial membranes. This study demonstrated that α-TP can restore the sensitivity of COL-R GNB to COL when used in combination.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Joule
·
2022
·
IF 46.048
Lingfeng Chao,Yingdong Xia,Xiaozheng Duan,Yue Wang,Chenxin Ran,Tingting Niu,Lei Gu,Deli Li,Jianfei Hu,Xingyu Gao,Jing Zhang,Yonghua Chen
Direct and stable α-phase formation of formamidinium lead triiodide (FAPbI 3 ) perovskite serves as an indispensable prerequisite for constructing stable and efficient perovskite solar cells. However, the α-phase formation is highly limited by molecular additives. Here, we report a strategy for directly fabricating stable α-phase of FAPbI 3 perovskite by controlling the precursor solution on the molecular level using methylammonium acetate (MAAc) ionic liquid. We found that a strong interaction between Ac − and Pb 2+ via C=O···Pb chelation enables the relaxation of Pb–I bonds and thus allows the effective insertion of formamidinium iodide (FAI) into soft Pb–I framework. Molecular dynamics simulations also show that the precursor solution has undergone an exchange of coordination between I − and Ac − to provide the reaction sites for FAI. With a one-step, antisolvent-treatment-free, and air-processing approach regardless of humidity, the corresponding photovoltaic cell exhibits an efficiency close to 24% along with long operational stability over 1,000 h.
✓ 已验证
DOI
高影响因子
Bioactive Materials
·
2025
·
IF 20.3
Yan Fu,Nan Lyu,Xia Tang,Ying Wang,Xiaohui Mou,Wentai Zhang,Siwei Yin,Mu Li,Na Bai,Nan Huang,Xin Li,Yifeng Lei,Jun Hou,Zhilu Yang
Timely endothelial regeneration and repair after stenting is essential for enhancing vascular healing performance. This necessitates that the stent surface be capable of maintaining a positive balance or sequential regulation among thrombosis, inflammation, and the growth behavior of smooth muscle cells (SMCs) and endothelial cells (ECs); however, a key challenge in achieving this goal remains. Herein, we report an immunomodulatory glutathione peroxidase (GPx) mimic-engineered α-lactalbumin self-assembling coating (LASC) for tailoring such surface multi-functionalities of a stent. The coating is achieved by first pre-coating an α-lactalbumin self-assembling nano-film driven by an oxidizing agent onto the stent surface, followed by covalent immobilization of GPx mimics (i.e., Cu II -tetraazamacrocyclic (Cu-DOTA) coordination complexes) onto the LASC matrix. Cu-DOTA-engineered LASC (Cu-DOTA/LASC) exhibits potent GPx-mimicking catalytic activity, substantially resists adsorption by blood components, and stably and continuously decomposes S-nitrosothiols into nitric oxide (NO) in blood. The long-acting release of NO could create an endothelium-mimicking microenvironment to prevent thrombosis formation, regulate inflammation, inhibit SMC proliferation, and enhance EC growth. As a supplement to the functions of NO, the α-lactalbumin exhibited synergistic effects on anti-thrombosis and immunoregulation. In vivo studies demonstrate that the Cu-DOTA/LASC-functionalized stents enabled rapid re-endothelialization, hence effectively reducing stenosis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2024
·
IF 5.1
Zhihao Xu,Hezhong Ouyang,Haoda Zhang,Lei Sun,Shasha Li,Ruijiang Liu
A highly sensitive label-free electrochemical aptamer biosensor with magnetically induced self-assembly had been developed to detect gastrin-releasing peptide precursor (ProGRP), which is not only a highly specific tumour marker for small-cell lung cancer (SCLC), but also very stable in serum, and thus had robust clinical utility. Based on the advantages of high specific surface area and facile separation and recycling, magnetic α-Fe 2 O 3 /Fe 3 O 4 nanorods with the average length of 175 nm, the average diameter of 28 nm, and the saturation magnetization of 66.9 emu/g were used as the matrix, and Au nanoparticles (AuNPs) were loaded on the surfaces of the nanorods to enhance the electrochemical signal. The aptamer double-stranded probe with high specificity to the target protein was bound to the surface of α-Fe 2 O 3 /Fe 3 O 4 @Au. The thiol-modified oligonucleotides could quickly and stably bind to AuNPs to form Au-S, which provided a guarantee for the immobilization of the probe and the controllable self-assembled molecular layer. Then, the sensor after capturing the target was self-assembled on the surface of the magnetic glassy carbon electrode (MGCE) by liquid phase incubation for electrochemical detection. The construction and detection performance of the sensor were evaluated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV). Under the optimized conditions, the sensor had remarkable linearity in the range of 0.1 pg/mL - 100 ng/mL (R 2 = 0.998), the limit of detection (LOD) was 17.51 fg/mL, while the limit of quantitation (LOQ) was 58.39 fg/mL. Additionally, the biosensor had excellent stability, reproducibility and anti-interference ability, as well as satisfactory recovery in spiked serum samples (99.46 %–103.32 %).
✓ 已验证
产品关联
DOI
高影响因子
ChemistrySelect
·
2025
·Vol. 10
·期 35
·e01362
·
IF 2
Changshun Lv,Xiangru Feng,Boyuan Jian,Xue Jiang,Tianhui Liu,Leijiao Li,Qingshuang Wang
One of the main challenges in wound healing is bacterial infection, and the emergence of antibiotic resistant superbugs in recent years has exacerbated these concerns. In this study, we developed a chitosan/tannic acid/poloxamer 407/poloxamer 188 thermo-sensitive hydrogel (CTPP). The bioactive compound α-Mangostin (α-M) extracted from mangosteen fruit was incorporated into CTPP (CTPP@α-M). The results indicate that CTPP@α-M can slowly and continuously release α-M within 11.5 h. In addition, CTPP@α-M effectively inhibits bacterial proliferation, accelerates collagen deposition, reduces inflammatory response, and promotes rapid wound healing in the presence of wound infection. The biosafety experiment showed that the hydrogel had excellent biocompatibility. Overall, these findings suggest that CTPP@α-M has the potential to become a potential treatment for repairing infectious wounds. Graphical In this study, CTPP hydrogel and CTPP @ α - M drug carrying hydrogel were prepared by physical crosslinking method. CTPP shows excellent performance and can be converted to semi-solid gel at 37 ° C. CTPP @ α - M showed strong inhibitory effects on MRSA and accelerated wound healing, reducing the expression of pro-inflammatory cytokines. The data show that CTPP @ α - M is a highly effective antibacterial thermosensitive hydrogel with excellent performance.
✓ 已验证
产品关联
DOI
高影响因子
Advanced Energy Materials
·
2023
·Vol. 13
·期 23
·2300451
·
IF 27.8
Jun He,Dewang Li,Hongli Liu,Junyan Xiang,Jiaxv Bai,Yuting Ren,Zhongyue Wang,Meng Xia,Xunjie Yin,Longfei Yuan,Fei Zhang,Shirong Wang
α -phase formamidine lead iodine ( α -FAPbI 3 ) as a competitive candidate to pursue high-performance perovskite solar cells (PSCs) suffers from the weakness of random nucleation and disordered growth in chasing high-grade polycrystalline films. Here, single-crystal seeds crystallization (SCSC) method is proposed to obtain high-quality α -FAPbI 3 perovskite films for the first time. Six single-crystals seeds (CsPbBr 3 , CsPbI 3 , FAPbBr 3 , FAPbI 3 , MAPbBr 3 , and MAPbI 3 ) are employed to regulate α -FAPbI 3 perovskite crystallization. These seeds act as nucleation sites and allow the perovskite to grow directly. The rapid crystallization significantly improves the crystallinity of perovskite films with uniform morphology, large grain size, and less defects. Of particular note, FAPbBr 3 seed prefers to grow α -FAPbI 3 epitaxially from FAPbBr 3 @PbI 2 heterostructure through a halogen anion exchange process. The average grain size of FAPbBr 3- perovskite film is boosted to 2.6 µm, and the trap density reduced by 11 times. The resultant FAPbBr 3 -PSCs achieve a maximum power conversion efficiency (PCE) of 23.84% with a high open-circuit voltage of 1.18 V. The unencapsulated PSC retains 91% of the initial PCE after 3000 h of storage at ≈30–50% relative humidity and preserves 98.5% of the original value after 155 h illumination with maximum power point tracking.
✓ 已验证
DOI
高影响因子
JOURNAL OF APPLIED ENTOMOLOGY
·
2022
·Vol. 146
·期 7
·823-837
·
IF 2.183
Xuanyu He,Zhaodong Hao,Hui Li,Tian Xu,Ruixu Chen,Xiaohong Xia,Shouyin Li,Dejun Hao
Understanding the mechanism of insect resistance to toxic effects of phytochemicals can provide an insight into plant–herbivore interactions. Monochamus alternatus Hope, a main vector of pine wood nematode ( Bursaphelenchus xylophilus ), prefers to infest masson pine ( Pinus massoniana Lamb) and causes huge economic and environmental losses. α-Pinene is the primary component of resin produced by masson pine and plays a crucial role in defending against herbivorous insects, but its defensive effects on M . alternatus larvae are barely known. Here, we explored the physiological metabolism in M . alternatus larvae that were fumigated with (+)-α-pinene, (-)-α-pinene, β-pinene, (R)-(+)-limonene, myrcene and (-)-α-phellandrene. (+)-α-Pinene showed the highest fumigant toxicity to M . alternatus larvae among these monoterpenes, and the fourth instar larvae possessed the highest resistance to this compound at the larval stage. Comparative transcriptome analysis detected 423 up-regulated and 311 down-regulated genes in the larvae fumigated with (+)-α-pinene, which were mainly related to detoxification, energy and protein metabolism. Among the differentially expressed genes encoding for detoxification enzymes, 14 P450s, 13 UGTs, 1GSTs and 3ABCs were dramatically up-regulated in the larvae fumigated with (+)-α-pinene. Silencing of P450s via RNA interference led to an increased mortality in the larval fumigated with (+)-α-pinene. Piperonyl butoxide exposure significantly increased the mortality and inhibited the expressions of P450s, UGTs and ABCs in the larvae fumigated with (+)-α-pinene. These results suggested an important role of P450s in the resistance of M . alternatus larvae to (+)-α-pinene fumigation, which may facilitate the understanding of terpenoid detoxification in M . alternatus larvae.
✓ 已验证
DOI
高影响因子
MATERIALS CHARACTERIZATION
·
2022
·
IF 4.537
Yahui He,Shihong Zhang,Yi He,Hongjie Li,Teng He,Ruxia Song,Bo Liu,Zhifei Zhang,Yi Fan
Ni-P/α-ZrP composite coatings were successfully prepared by electroless plating, in which α-ZrP nanosheets were synthesized by reflow method. The surface morphology, element composition, structure and valence composition of the composite coating were analyzed by SEM, EDS, XRD and XPS, respectively. In the reciprocating friction experiment and electrochemical test, the corrosion and wear behaviour of the composite coatings were analyzed. The results show that α-ZrP nanosheets can significantly improve the corrosion and wear resistance of Ni P alloys. With the enrichment of α-ZrP nanosheets, the properties of coatings showed a tendency of increasing first and then slightly decreasing. Ni-P/α-ZrP (1.00 g/L) coating has the lowest porosity and the best corrosion resistance, and its corrosion rate (6.01 × 10 −3 mm/a) is about 1/5 of that of pure Ni P coating (3.12 × 10 −2 mm/a). Ni-P/α-ZrP (1.50 g/L) coating has the best wear performance, its average coefficient of friction (COF) is reduced from 0.568 to 0.167, and the specific wear rate is reduced from 7.13 ± 0.42 (× 10 −6 ) to 0.36 ± 0.06 (× 10 −6 ) (about 1/20 of pure Ni P coating).
✓ 已验证
DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2022
·Vol. 102
·期 10
·4065-4078
·
IF 4.125
Huwei Wu,Linping Shu,Tian Liang,Yanping Li,Yuanxiang Liu,Xiuli Zhong,Lingyu Xing,Wei Zeng,Rui Zhao,Xiaoyin Wang
BACKGROUND Lotus seedpods are an agricultural by-product of lotus ( Nelumbo nucifera Gaertn.), which is widely cultivated in Southeast Asia and Australia. Most lotus seedpods are considered waste and are abandoned or incinerated, resulting in significant waste of resources and heavy environmental pollution. For recycling lotus seedpods, the extraction optimization, physicochemical properties, antioxidant activity, and α -glucosidase inhibitory effect of the polysaccharides contained therein were investigated in this study. RESULTS Hot water extraction of lotus seedpod polysaccharides was optimized by using a response surface methodology combined with a Box–Behnken design, with the optimum conditions being as follows: a liquid/solid ratio of 25.0 mL g −1 , an extraction temperature of 98.0 °C, and an extraction time of 138.0 min. Under these conditions, an experimental yield of 5.88 ± 0.06% was obtained. Physicochemical analyses suggested that lotus seedpod polysaccharides belong to acidic heteropolysaccharides and are principally composed of rhamnose, arabinose, galactose, glucose, mannose, and galacturonic acid. The polysaccharides content has a broad molecular weight distribution (2.15 × 10 5 to 1.77 × 10 7 Da), an α -configuration, and mainly possesses smooth and sheet-like structures. Biological evaluations showed that the polysaccharides possessed good scavenging activity on 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, 1,1-diphenyl-2-picryl-hydrozyl, and hydroxyl radicals, and exerted an obvious inhibitory effect on α -glucosidase activity. Moreover, the polysaccharides content was determined to be a mixed-type noncompetitive inhibitor of α -glucosidase. CONCLUSION The results indicate that lotus seedpod polysaccharides have potential as natural antioxidants and hypoglycaemic substitutes. This study provides the theoretical bases for the exploitation and application of polysaccharides from lotus seedpod by-product resources. © 2022 Society of Chemical Industry.
✓ 已验证
DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
·
2021
·Vol. 18
·期 10
·e2100244
·
IF 2.408
Du-Xin Jin,Jun-Fang He,Ke-Qin Zhang,Xue-Gang Luo,Tong-Cun Zhang
Hypericum attenuatum Choisy is a traditional Chinese herbal plant with multiple therapeutic effects. In this study, bioactivity-guided fractionation of Hypericum attenuatum Choisy extracts afforded three major flavonoids (including astragalin, guaijaverin and quercetin), which possessed α-Glucosidase inhibitory activity with IC 50 values of 33.90±0.68 μM, 17.23±0.75 μM and 31.90±0.34 μM, respectively. Circular dichroism analysis revealed that all the three compounds could interact with α-glucosidase by inducing conformational changes of the enzyme. Molecular docking results indicated that they could bind to the active site in α-glucosidase, and the binding force was driven mainly by hydrogen bond. Additionally, isobolographic analysis of the interactions between two compounds showed that all the combinations presented a synergistic α-glucosidase inhibitory effect at lower concentrations, and the combination between quercetin and guaijaverin or astragalin exhibited the best synergistic effect. This research might provide a theoretical basis for the application of Hypericum attenuatum Choisy in treating hyperglycemia.
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）