MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 10
·1-9
·
IF 6.232
Liu Hui,Liu Bowen,Huang Pengcheng,Wu Yangyang,Wu Fang-Ying,Ma Lihua
Gold nanoparticles (AuNPs) catalyze the mild reaction between the weak reducing agent kojic acid (KA) and silver ions (Ag + ) to form Au@Ag bimetallic NPs by the combination of the intrinsic catalysis with plasmonic properties This is proposed as a novel optical assay to determine the tyrosinase (TYRase) concentration. The nanoparticles have been characterized by UV-vis spectroscopy, transmission electron microscope (TEM) images, and X-ray photoelectron spectroscopy (XPS). The sensing mechanism is based on the fact that KA binds to TYRase by chelating with dicopper active site of TYRase and the introduction of TYRase restrains the Au@Ag bimetallic NP formation by the precedent binding with KA. A clear color variation from yellow to pink and UV-vis spectral changes are observed at the optimal wavelength of 410 nm. The assay works in the range 0.13~0.73 U mL −1 with a detection limit (LOD) of 0.019 U mL −1 . The impact from matrix interfering substances including glucose, uric acid, common oxidases, and amino acids is negligible. The applicability is demonstrated by quantitative determination of TYRase in human serum samples with 74 to 89% recovery and RSD less than 4.0%, which accords with the level for bio-sample analysis.
Schematic presentation of colorimetric assay for tyrosinase (TYRase) based on the inhibition effect on silver deposition onto catalytically active gold nanoparticles (AuNPs) and its application with a smartphone. Tyrosinase (TYRase); silver ions (Ag + ); kojic acid (KA); gold nanoparticles (AuNPs); gold-silver core-shell nanoparticles (Au@Ag NPs)
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高影响因子
MOLECULAR NEUROBIOLOGY
·
2020
·Vol. 57
·期 12
·5324-5335
·
IF 4.5
Chen Guang,Gao Cheng,Yan Ya’nan,Wang Tao,Luo Chengliang,Zhang Mingyang,Chen Xiping,Tao Luyang
Intracerebral hemorrhage (ICH) is a form of stroke, characterized by high morbidity and mortality and currently lacks specific therapy. ICH leads to endoplasmic reticulum (ER) stress, which can induce neurological impairment through crosstalk with programmed cell death (PCD). Pyroptosis, a newly discovered form of PCD, has received attention because of its close relationship with some certain diseases, such as traumatic brain injury and ischemic and hemorrhagic stroke. However, the relationship between ER stress and pyroptosis in ICH remains unclear. In this study, we investigated the role of ER stress in evoking neuronal pyroptosis and related mechanisms in a mouse ICH model. We used tauroursodeoxycholic acid (TUDCA) to inhibit ER stress and observed that TUDCA reduces neuronal pyroptosis and has a neuroprotective role. We explored the potential mechanisms underlying the regulation of neuronal pyroptosis by ER stress through testing the expression of interleukin-13 (IL-13). We found that ER stress inhibition alleviates neuronal pyroptosis through decreasing the expression of IL-13 after ICH. In summary, this study revealed that IL-13 is involved in ER stress–induced neuronal pyroptosis after ICH, pointing to IL-13 as a novel therapeutic target for ICH treatment.
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IONICS
·
2020
·Vol. 26
·期 12
·5897-5906
·
IF 2.394
Gu Fengling,Liu Wenbin,Song Yonghai,Liu Li-Min,Zhu Yongmei
Herein, a new activated porous carbon (APC) was proposed by carbonizing waste biomass of pinecone which could be obtained in large quantities and subsequently activation of zinc chloride. Then small Co 3 O 4 nanoparticles were loaded on the surface and holes of APC to prepare the final Co 3 O 4 /APC nanocomposites. The as-prepared APC had a lotus root-like and layered porous structure with a large number of mesopores and micropores. The APC reduced the agglomeration of small Co 3 O 4 particles which were uniformly anchored on the surface of APC. When applied for the anode of lithium-ion batteries, the Co 3 O 4/ APC nanocomposites had a reversible capacity of 625 mAh g −1 after 200 cycles at the current density of 500 mA g −1 . The Co 3 O 4/ APC nanocomposites also had a good rate performance. Above all, this work could transform waste biomass into sustainable resource, and the raw materials were easy to obtain in large quantities.
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Nano Research
·
2020
·Vol. 13
·期 4
·959-966
·
IF 8.183
Wang Lin,Yue Qiuyan,Pei Chengjie,Fan Huacheng,Dai Jie,Huang Xiao,Li Hai,Huang Wei
Recently, transition metal dichalcogenides (TMDCs) nanoscrolls have exhibited unique electronic and optical properties due to their spiral tubular structures, which are formed by rolling up monolayer TMDCs nanosheets. Inspired by the excellent physical and chemical properties of TMDCs van der Waals heterostructures (vdWHs), it is highly desirable to scroll TMDCs vdWHs for potential optoelectronic applications. In this work, WS 2 /MoS 2 vdWHs nanoscrolls were massively prepared by dropping aqueous alkaline droplet on chemical vapor deposition (CVD)-grown bilayer WS 2 /MoS 2 vdWHs, which were formed by growing monolayer WS 2 islands on top of monolayer MoS 2 nanosheets simultaneously. The optical microscopy (OM), atomic force microscopy (AFM), ultralow frequency (ULF) Raman spectroscopy and transmission electron microscopy (TEM) were utilized to characterize the WS 2 /MoS 2 vdWHs nanoscrolls. As-obtained WS 2 /MoS 2 vdWHs nanoscrolls exhibited new ULF breathing mode as well as shear mode peaks due to the strong interlayer interaction. Notably, the photosensitivities of WS 2 /MoS 2 vdWHs nanoscrolls-based devices were about ten times higher than those of WS 2 /MoS 2 vdWHs-based devices under blue, green and red lasers, respectively, which could be attributed to the ultrafast charge transfer at alternative WS 2 /MoS 2 and MoS 2 /WS 2 multi-interfaces in scrolled structure. Our work suggested that TMDCs vdWHs scrolls could be promising candidates for optoelectronic applications.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2020
·Vol. 27
·期 14
·17043-17053
·
IF 3.056
Lin Wenting,Zhang Xiaohan,Li Ping,Tan Yongzhen,Ren Yuan
As a commonly used anti-diabetic drug, metformin (MEF) is frequently detected in different water bodies which pose a potential threat to human health and the ecological environment. In this study, oxidative degradation of MEF under ultraviolet (UV) light was studied, and its influencing factors, photolysis mechanism, and intermediates identification carried out as well. The results showed that the hydroxyl radical contributed 73% during the 6 h MEF photolysis process among the reactive oxygen species (ROS). In addition, triplet excited-state organic matter and singlet oxygen also played a role in the photolysis process. The reaction rates of hydroxyl radical and singlet oxygen with MEF are (6.45 ± 0.4) × 10 9 and (5.4 ± 0.7) × 10 6 L·(mol s) −1 , respectively. By calculating the light screening effect of environmental factors, it is found that the presence of NO 3 − and Cl − had a greater excitation effect on ROS than the screening effect, and generally promoted the photolysis rates of MEF from 90.3 to 193.5% and from 16.1 to 80.6% during the 6-h reaction process, respectively. For bicarbonate and fulvic acid, the light screening effects were dominant and inhibited photolysis rates by 10–52% and 13–71%, respectively. The results demonstrated that the photoreactivity of environmental factors in water is the cause of the different photodegradation rates of MEF. The oxidative degradation product of MEF under UV light was detected by UPLC/Q-TOF as methylbiguanide (MBG), 2,4-diamino-1,3,5-triazine (2,4-DAT), biguanide (BGN), 1,1-dimethylguanidine (1,1-DiMBG), 4-amino-2-imino-1-methyl-1,2-dihydro-1, 3,5-triazine (4,2,1-AIMT), and 2-amino-4-methylamino-1,3,5-triazine (2,4-AMT). The result which showed that the primary sites of ∙OH attacked is consistent with that of density functional theory calculation.
Graphical abstract
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高影响因子
CELLULOSE
·
2020
·Vol. 27
·期 7
·3989-4005
·
IF 4.21
Li Shengnan,Huang Shuo,Xu Fang,Zhao Tianfu,Zhang Fengxiu,Zhang Guangxian
Abstract An eco-friendly hydrophobic flame retardant, ammonium salt of octadecyl phosphate (AMOP) was synthesized and its structure was verified by 1 HNMR, 13 CNMR and 31 PNMR. AMOP imparted flame retardancy and superhydrophobicity to cotton fabrics simultaneously. The water contact angles and limiting oxygen index values of treated cotton reached 161.8° and 27%, respectively. After 30 laundering cycles, the hydrophobicity sustained very well. Thermogravimetric analysis and thermogravimetric-infrared spectroscopy suggested AMOP changed the thermal decomposition path of cotton fibers and the treated sample released less flammable gas during the thermal decomposition process. Cone calorimetry results showed the total heat release and the heat release rate of treated cottons were much lower than those of control cottons. Fourier-transform infrared spectroscopy, energy dispersive X-ray analysis and X-ray photoelectron spectroscopy suggested the AMOP successfully grafted on cotton fabrics through P-O-C groups. Scanning electron micrography showed AMOP caused a rough surface.
Graphic abstract
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MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 3
·1-8
·
IF 6.232
Mei He,Shu Huawei,Lv Mengyu,Liu Wei,Wang Xuedong
Phenyl-modified graphitic carbon nitride nanosheets (Ph- g -C 3 N 4 NSs) were synthesized by a thermal copolymerization and ultrasonic exfoliation method. The Ph- g -C 3 N 4 NSs are used as a fluorescent assay for determination of thiram. The results of X-ray photoelectron spectroscopy, 13 C solid-state nuclear magnetic resonance and Fourier transform infrared spectra confirm that phenyl group is integrated into the heptazine network of g -C 3 N 4 . Compared to the g -C 3 N 4 NSs, the Ph- g -C 3 N 4 NSs show bigger stokes shift about 185 nm and higher fluorescence intensity. The fluorescence of Ph- g -C 3 N 4 NSs is quenched by Cu 2+ via the photo-induced electron transfer mechanism, which then recovers in the presence of thiram. The fluorescence restoring of Ph- g -C 3 N 4 NSs is correlated with the concentration of thiram. Under the optimized conditions, the fluorescent intensity of g -C 3 N 4 NSs at excitation/emission wavelengths of 310/455 nm give a linear range of 33.0–670 nM with detection limit of 9.90 nM. While fluorescent assay based on the Ph- g -C 3 N 4 NSs show the linear range of 6.70–1300 nM at excitation/emission wavelengths of 310/495 nm with detection limit of 2.01 nM.
Schematic representation of fluorescent “on-off-on” assay based on phenyl-modified graphitic carbon nitride nanosheets (Ph- g -C 3 N 4 NSs) for determination of thiram.
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高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 412
·期 8
·1915-1923
·
IF 3.637
Ge Minghao,Sun Jiaojiao,Chen Mingli,Tian Jingjing,Yin Huancai,Yin Jian
Due to its important role in tumor development and treatment, hyaluronidase (HAase) has been widely investigated in vitro and in vivo. However, such investigation was limited by the absence of sensitive and in situ detection methods. Herein, a hyaluronic acid (HA) hydrogel based on the fluorescence resonance energy transfer (FRET) effect was constructed for the detection of HAase. FITC and AuNPs were covalently coupled with two HA derivatives respectively to form a fluorescent donor-acceptor pair. In the presence of HAase, the hydrogel established by cross-linking of HA derivatives was hydrolyzed specifically. The FRET effect in the hydrogel disappeared and the fluorescence intensity increased proportionally with the changes in the concentration of the HAase. Experiments proved that the HAase sensing system had a wide response range (0.5–100 U/mL), good anti-interference, and excellent biocompatibility. When the hydrogel was used for 3D culture of lung cancer cells, in situ fluorescent response could be achieved.
Graphical abstract
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高影响因子
Scientific Reports
·
2020
·Vol. 10
·期 1
·1-12
·
IF 3.998
Akram Naeem,Guo Jia,Ma Wenlan,Guo Yuan,Hassan Afaq,Wang Jide
The exploration of advanced water treatment technologies e.g. heterogeneous photocatalysis is the most promising way to address organic pollution issues. Semiconductors based bimetallic photocatalysis with wide bandgap, have displayed splendid degradation performance in the UV light region, but their extension to the visible light/near infra-red region is still a matter of great concern. CuO, Co(OH) 2 , CoO and Co(OH) 2 /CuO nanocomposites were synthesized via simple co-precipitation method and further practiced for Rhodamine B (RhB) decomposition by introducing per-sulfate (PS) as a sacrificial agent. Results revealed that Co(OH) 2 /CuO catalyst had shown robust catalytic activity for RhB photodegradation (degradation time 8 min, k = 0.864 min −1 ) under light illumination, significantly less (12–60 times) than the other reported bimetallic catalysts. Catalyst also have verified excellent performance for a broader pH range (5–9) with excellent stability. Main reactive species responsible for the photocatalytic reaction were sulfate (SO 4 •− ) and superoxide (O 2 • ) radicals, duly verified by ESR and by using radical scavengers. With outstanding recycling abilities, this is probably the fewer successful attempt for RhB decolorization and can be highly favorable for effluent treatment by using the synergic effect of absorption and photodegradation.
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高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2020
·Vol. 193
·期 1
·205-217
·
IF 2.277
Wan Lijun,Gao Zhen,Wu Bin,Cao Fei,Jiang Min,Wei Ping,Jia Honghua
Corncob is an abundant agricultural residue containing high content of hemicellulose. In this paper, the hemicellulosic hydrolysate was prepared from the hydrolysis of corncob using the solid acid sulfated zirconia as a catalyst. According to response surface analysis experiments, the optimum conditions for preparing hemicellulosic hydrolysate catalyzed by sulfated zirconia were determined as follows: solid (sulfated zirconia)–solid (corncob) ratio was 0.33, solid (corncob)–liquid (water) ratio was 0.09, temperature was 153 °C, and time was 5.3 h. Under the optimized conditions, the soluble sugar concentration was 30.12 g/L with a yield of 033 g/g corncob. Subsequently, xylitol production from the resulting hemicellulosic hydrolysate was demonstrated by Candida tropicalis , and results showed that the yield of xylitol from the hemicellulosic hydrolysate could be significantly improved on a basis of decolorization and detoxification before fermentation. The maximum yield of xylitol from the hemicellulosic hydrolysate fermented by C. tropicalis was 0.76 g/g. This study provides a new attempt for xylitol production from the hemicellulosic hydrolysate.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2020
·Vol. 27
·期 33
·41437-41445
·
IF 3.056
Zhu Chao,Wang Huiqin,Ma Hongrui,Yang Yonglin,Li Fan
Humic-like substances are essential components of soluble organic matter in tannery wastewater. However, the tannery process can promote the abiotic humification in wastewater. Therefore, it is of great significance to clarify the pathway and degree of abiotic humification and the properties of the as-derived humic acid-like (HAL) complex polymers in the tannery process in order to control the refractory organic compounds. In the present study, considering the catechol-Maillard system and commercial humic acid (HA) as control, the polyphenol-Maillard humification in the tannery process was simulated under the catalysis of MnO 2 . Moreover, physicochemical and spectroscopic techniques were used to characterize the separated fractions of HAL further. As a result, it was found that the catechol-Maillard system with small molecule organic matter as precursor had higher humification degree. Furthermore, the ultraviolet-visible (UV-Vis), Fourier transform infrared (FTIR), and excitation-emission matrix (EEM) fluorescence spectrum of humic acid-like 0 (HAL0) derived from it was different from those of humic acid-like 1 and 2 (HAL1 and HAL2) of polyphenol-Maillard system, indicating the differences of polymer structure between them. In the polyphenol-Maillard system, tannin was the skeleton of polymerization or polycondensation reaction, and the high content of N and the H/C value of HAL2 indicated that in adding to amino acids, proteins promoted the humification, forming industry-specific HAL polymers with a high degree of aliphatic nature. Therefore, it can be concluded that controlling the raw materials in the tannery process (especially tannins), in order to reduce the occurrence of abiotic humification may be the key to improve the efficiency of wastewater treatment.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2020
·Vol. 18
·期 1
·1-13
·
IF 6.518
Wu Yiyong,Deng Peihong,Tian Yaling,Feng Jinxia,Xiao Jingyun,Li Junhua,Liu Jun,Li Guangli,He Quanguo
A method with high sensitivity, good accuracy and fast response is of ever increasing importance for the simultaneous detection of AA, DA and UA. In this paper, a simple and sensitive electrochemical sensor, which based on the polyvinylpyrrolidone (PVP)-graphene composite film modified glassy carbon electrode (PVP-GR/GCE), was presented for detecting ascorbic acid (AA), dopamine (DA) and uric acid (UA) simultaneously. The PVP-GR/GCE has excellent electrocatalytic activity for the oxidation of AA, DA and UA. The second-order derivative linear sweep voltammetry was used for the electrochemical measurements. The peak potential differences of DA-AA, DA-UA, and UA-AA (measured on the PVP-GR/GCE) were 212, 130 and 342 mV respectively. Besides, the over potential of AA, DA and UA reduced obviously, so did the peak current increase. Under the optimum conditions, the linear ranges of AA, DA and UA were 4.0 μM–1.0 mM, 0.02–100 μM, and 0.04–100 μM, respectively. The detection limits were 0.8 μM, 0.002 μM and 0.02 μM for AA, DA, and UA. The electrochemical sensor presented the advantages of high sensitivity and selectivity, excellent reproducibility and long-term stability. Furthermore, the sensor was successfully applied to the analysis of real samples.
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高影响因子
危险品化学品经营许可证（带存储）