SENSORS AND ACTUATORS B-CHEMICAL
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2021
·
IF 7.46
Xuechi Yin,Leina Dou,Huilan Hu,Ting Du,Sijie Liu,Man Zhao,Yanru Wang,Jianlong Wang,Daohong Zhang
Here, to realize the simultaneous, simple, and highly sensitive detection for nitrofurazone and furazolidone, an immune-scaffold relying immunochromatographic assay was proposed. In this multi-detection immunochromatographic assay (MDICA), the immune-scaffold is composed of labeled anti-antibody IgG (Ab 2 ) and two anti-analyte monoclonal antibodies (Abs 1 ). A nanocomposite of MIL-101 (Cr)@AuNPs is used as the signal tag, where the unique optical property and biocompatibility of AuNPs and the protective effect of metal-organic framework can enable the nanocomposite possesses powerful signals, conjugating ability with antibodies and enhanced colloidal stability. In the immune-scaffold platform, the labeled Ab 2 is the core trestle, on the one hand to exempt Ab 1 from labeling requirement and offer strong detection signals, on the other hand to orient Abs 1 to expose their antigen-binding sites as well as improve the identification and binding efficiency between antigen and antibody. Compared with randomly fixed antibody in the conventional probe, a 6-fold reduction of applied Ab 1 amount can be achieved to produce the similar signal intensity in the immune-scaffold based method. In brief, the proposed MDICA system can effectively simplify the construction steps of MDICA, improve the Abs 1 utilization efficiency, heighten analytical performances, and providing an efficient, valuable reference for the establishment of other MDICAs.
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高影响因子
IONICS
·
2021
·Vol. 27
·期 11
·4885-4895
·
IF 2.817
Qu Yuning,Yu Zehui,Wei Dan,Chen Ling,Zheng Zhe,Xiao Ningru,Wang Lili,Yu Jianguo
Pt-based materials have widely been explored as hydrogen evolution reaction (HER) catalysts, but the high cost of Pt catalyst limits its application in HER. Therefore, we report a platinum/polyvinyl-alcohol (Pt x -PVA) catalyst with high catalytic activity and low Pt loading. Pt x -PVA catalysts with a uniform distribution of the Pt nanoparticles (NPs) have successfully been synthesized by a simple one-step hydrothermal method. The size and morphology of Pt NPs can be easily adjusted by changing the amount of Pt precursor, easy to pick out the sample with the best performance. Among them, the Pt 1.88 -PVA catalyst exhibits the best electrocatalytic activity for HER in 0.5 M H 2 SO 4 solution. An overpotential of 34 mV on Pt 1.88 -PVA catalyst is obtained at a current density of −10 mA cm −2 , which is slightly better than the overpotential of the commercial Pt/C (42 mV). Similarly, the Tafel slope of 31 mV dec −1 on the Pt 1.88 -PVA catalyst indicates that the HER process is the Volmer-Tafel mechanism. However, the Pt loading of the Pt 1.88 -PVA catalyst is 18.8% times lower than that of the commercial Pt/C. Furthermore, the Pt x -PVA catalysts exhibit an excellent stability and durability. This is due to the coating effect of PVA for Pt NPs, which limits the migration and agglomeration of Pt NPs. This study provides a new approach for reducing the Pt load in Pt-based catalyst in the field of electrochemistry.
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高影响因子
TALANTA
·
2021
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IF 6.057
Yue Sun,Zhenzi Xu,Feifang Zhang,Zongying Li,Bingcheng Yang
We describe a reagent-free acid-base titration method, in which only water is added and the titrant is online electrodialytically produced. Electrodialytic eluent generator, a well-established technique in ion chromatography to produce high purity base or acid eluent through precise control of an electric current, has been for the first time used for titration, termed as electrodialytic titrant generator (ETG). Three kinds of titrants produced by ETG have been demonstrated, including potassium hydroxide , methanesulfuric acid and sulfuric acid. A series of titrants with different concentration up to at least 140 mM (e.g. KOH) could be accurately and reproducibly obtained by manipulating current and the KOH titrant showed extremely high purity. The titration results achieved by ETG were in a good agreement with those obtained by the regular way and their ratio was in the range of 0.9918 and 1.0034. Good accuracy and precision were achieved for ETG titration, as indicated by 2.2% of relative error and 0.17% of relative standard error. Its utility was demonstrated to measure pKa-differentiated capacity of anion exchange resins .
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高影响因子
TRIBOLOGY INTERNATIONAL
·
2021
·
IF 4.872
Hongxiang Yu,Zhiwen Zheng,Haijie Chen,Dan Qiao,Dapeng Feng,Zhenbin Gong,Guojun Dong
The tribochemical reaction rate of multiply alkylated cyclopentane (MAC) was studied from the perspective of tribo-oxidation. The activation energy of MAC oxidation reaction and respective decrease of activation energy owing to catalysis from iron and Fe 3 O 4 were determined by means of auto-oxidative tests. The sum of the reduced activation energy due to iron and Fe 3 O 4 was less than that in tribological test, which highlighted the crucial role of shear stress in accelerating tribo-oxidation reaction. Additionally, the experimental process was replicated by utilizing reactive molecular dynamics (MD) simulations. The simulation results revealed the significance of shear stress in tribo-oxidation reaction and the catalytic mechanism of iron, Fe 3 O 4 , and shear stress for promoting oxidation reaction was interpreted.
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 10
·1-9
·
IF 5.479
Yi Haoyang,Yan Zhiyu,Wang Lumei,Zhou Xiaotong,Yan Rui,Zhang Dongwei,Shen Guoqing,Zhou Shanshan
A fluorometric method is described for the determination of ofloxacin (OFL). It is based on the use of the fluorescent intercalator SYBR Green I (SG-I). The OFL-aptamer has G-quadruplex structures and can be recognized by SG-I. It results in strong fluorescence of SG-I. If OFL is present, OFL will bind to its aptamer to form stable complexes. This induces the despiralization of partial dsDNA regions, leads to changes in the structure of the aptamer. Thus, SG-I is released from the OFL-aptamer into solution. Hence, the fluorescence of SG-I drops. Fluorescence decreases linearly in the 1.1 to 200 nM OFL concentration range, and the limit of detection is 0.34 nM. The method shows good selectivity to much interference including analogues, hormones, pesticides. It is also effortless and fast with the times of measurement of <40 min. In addition, good recoveries of 91.3–119.0% were found for tap water, river water and artificial urine spiked with OFL with relative standard deviation (RSD) of ≤11.6%.
A sensitive fluorometric method is developed for ofloxacin (OFL) detection in aqueous samples based on the fluorescence intensity change of SYBR Green I (SG-I) with or without OFL.
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International Journal of Polymer Science
·
2019
·
IF 1.892
Weiping Du,Guiming Zhang,Song Wang,Lianjiang Tan,Huifang Chen
An optical polythiourethane based on m-xylylene diisocyanate (XDI) and 4-mercaptomethyl-3,6-dithia-1,8-octanedithiol (BES) has been studied. Triethylamine was adopted as a catalyst, and the solid-state isothermal cure reaction was carried out using FTIR spectroscopy, in the temperature range of 75°C-105°C. The -NCO absorption band of XDI was used to monitor the conversion of diisocyanate into polythiourethane. The reaction rate enhanced with an increase in the content of the catalyst, and the gel time determined by swelling test was shorter for the system with higher catalyst content. Kinetic parameters were calculated from the infrared spectrum data, and the results showed that the curing reaction of polythiourethane accords with first-order kinetic characteristics. The activation parameters obtained from the evaluation of kinetic data were , , and . The observed negative entropy of activation value supported the formation of a transition state in the cure reaction.
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JOURNAL OF CHEMICAL RESEARCH
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2019
·
IF 0.67
He Li,Yijun Zhang,Dinghua Liu,Xiaoqin Liu
2,4-Dichlorophenylacetic acid is synthesized in high yield via the carbonylation of 2,4-dichlorobenzyl chloride, and various experimental conditions are evaluated. Xylene, bistriphenylphosphine palladium dichloride, tetraethylammonium chloride and sodium hydroxide in solution are added to the reaction system and held at 80 °C under a CO atmosphere. 2,4-Dichlorophenylacetic acid is obtained in a maximum yield of 95%, and a mechanism for 2,4-dichlorobenzyl chloride carbonylation is proposed. The reaction system provides a mild, effective and novel means by which to prepare phenylacetic acid derivatives from their corresponding benzyl chloride derivatives.
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BIOELECTROCHEMISTRY
·
2020
·
IF 4.722
Yafei Chen,Yajie Yang,Yu Wang,Ye Peng,Jinmei Nie,Guanyue Gao,Jinfang Zhi
Mycotoxin contamination in food and feed is a global concern because mycotoxin contamination can cause both acute and chronic health effects in humans and animals. In the present work, an Escherichia coli -based biosensor is described for the toxicity assessment of aflatoxin B 1 (AFB 1 ) and zearalenone (ZEN). In this electrochemical biosensor, E. coli is used as the signal recognition element, p -benzoquinone is used as the mediator, and a two-step reaction procedure has been developed to separate the mediator from the mycotoxins. The current value of the as-prepared microbial biosensor exhibits a linear decrease with concentrations of AFB 1 and ZEN in the range of 0.01–0.3 and 0.05–0.5 μg/mL, with detection limits reaching 1 and 6 ng/mL, respectively. The IC 25 values obtained by the present method are 0.25 and 0.40 μg/mL for AFB 1 and ZEN, which shows that the cytotoxicity of AFB 1 to E. coli is more severe than the cytotoxicity of ZEN to E. coli . The combined toxic effect of these two mycotoxins has also been explored, and synergistic biotoxicity has been observed. Moreover, the biosensor is successfully applied to the toxicity evaluation of mycotoxins in real samples, including peanut and corn oils. This work could provide new insight into mycotoxin and microorganism interactions and could establish a new approach for future mycotoxin detection.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2019
·
IF 3.375
Biaobiao Ma,Shiyong Sun,Huichao He,Rui Lv,Jianjun Deng,Tingting Huo,Yulian Zhao,Haili Yu,Lei Zhou
Photocatalysis is a green and sustainable technique for converting solar energy into energy-storage molecules. A novel type of metal-free photocatalyst consisting of polydopamine (PDA) surface-functionalized graphitic carbon nitride (g-C3N4) was fabricated to enable the highly efficient visible-light-driven photocatalytic production of reduced nicotinamide adenine dinucleotide (NADH) through the photomediated reduction of NAD+. The photocatalytic performance and kinetic processes were investigated to evaluate the efficiency of NADH regeneration by the PDA/g-C3N4 photocatalysts. It was shown that the PDA layer thus formed could promote the charge carrier transfer of g-C3N4 to increase the solar-to-chemical conversion efficiency under visible light irradiation. A photocurrent density three times higher than that of pristine g-C3N4 was obtained. The photocatalytic activity of the PDA/g-C3N4 photocatalysts appeared to be dependent on the pH, with an increase in the pH from 3.0 to 9.5 substantially enhanced the NADH yield. The 20 wt % PDA/g-C3N4 exhibited remarkably improved photocatalytic NADH regeneration efficiency, achieving an NADH yield of 94.7% with an initial reaction rate of 2.4 mmol·h–1·g–1, which is approximately six times higher than that of pristine g-C3N4. Our study thus identifies a promising photocatalyst for enhancing the photocatalytic efficiency for nicotinamide cofactor regeneration.
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BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Xi Yang,Wenxiong Shu,Yanqiong Wang,Yuan Gong,Chaoyang Gong,Qiushu Chen,Xiaotian Tan,Gang-Ding Peng,Xudong Fan,Yun-Jiang Rao
Turbidimetric inhibition immunoassay (TIIA) is a classic immunodiagnostic method that has been extensively used for biomarker detection. However, the low sensitivity of this technique hinders its applications in the early diagnosis of diseases. Here, a new concept, optofluidic laser TIIA (OFL-TIIA), is proposed and demonstrated for sensitive protein detection. In contrast to the immunoreaction in traditional TIIA, in which the single-pass laser loss is detected, the immunoreaction in the OFL-TIIA method takes place in a laser cavity , which considerably increases the loss induced by antigen-antibody complexes (AACs) via the amplification effect of the laser. A commercial IgG TIIA kit was selected as a demonstrative model to characterize the performance of OFL-TIIA. A wide dynamic range of five orders of magnitude with an exceptional limit of detection (LOD) (1.8 × 10 −10 g/L) was achieved. OFL-TIIA is a fast, sensitive, and low-cost immunoassay with a simple homogeneous and wash-free process and low-volume sample consumption, thus providing a new detection platform for disease diagnostics.
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高影响因子
Nanoscale
·
2019
·Vol. 11
·期 18
·8898-8905
·
IF 6.97
Luhong Fu,Xin Hu,Yunbo Li,Gongzhen Cheng,Wei Luo
Developing highly efficient and durable electrocatalysts for overall water splitting over a wide pH range is of great interest for practical applications, but still remains a challenge. Specifically, to the best of our knowledge, a 3-in-1 electrocatalyst that can efficiently catalyze overall water splitting in acidic, alkaline, and neutral electrolytes has not been reported so far. Herein, we report the colloidal synthesis of well-dispersed IrW nanobranches with branch architectures and describe how the morphology varies with the amount of W doping. As expected, they exhibit outstanding catalytic performance and durability for the hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) at all pH values, which are much higher than those of Ir nanoparticles (NPs), and most reported state-of-the-art electrocatalysts. More importantly, when further used as both an anode and cathode for overall water splitting in 0.1 M HClO4, 0.1 M KOH, and 1.0 M PBS (phosphate buffer solution), cell voltages of 1.58, 1.60, and 1.73 V, respectively, were achieved at a current density of 10 mA cm−2. The present work opens up a new avenue for designing electrocatalysts for pH-universal overall water splitting, especially for application in highly corrosive acidic media and neutral media with limited ionic concentrations.
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Scientific Reports
·
2019
·Vol. 9
·期 1
·1-8
·
IF 4.011
Wang Xiangnan,Liu Tao,Yu Jingkun
(4YSZ) 0.93 (Fe 2 O 3 ) 0.07 and 9 mol% Y 2 O 3 stabilized ZrO 2 (9YSZ) were synthesized by co-precipitation method and their crystalline structure, microstructure, electronic conductivity, total conductivity were characterized. A limiting current oxygen sensor was assembled with (4YSZ) 0.93 (Fe 2 O 3 ) 0.07 dense diffusion barrier and 9YSZ solid electrolyte by Pt sintered-paste method. Influences of temperature ( T ), oxygen concentration ( x (O 2 )) and water vapor pressure ( p (H 2 O)) on sensing characteristics of the limiting current oxygen sensor were investigated. The crystalline structure of (4YSZ) 0.93 (Fe 2 O 3 ) 0.07 and 9YSZ belong to cubic structure with \({\bf{Fm}}\overline{{\bf{3}}}{\bf{m}}\) . The total conductivity of (4YSZ) 0.93 (Fe 2 O 3 ) 0.07 is higher than that of 9YSZ and the electronic and total conductivities of the samples meet the linear relationship with 1000/ T . The limiting current oxygen sensor exhibits excellent sensing characteristics under test conditions. The effects of T , x (O 2 ) and p (H 2 O) are as follows: Log( I L · T ) depends linearly on 1000/ T , I L depends linearly on x (O 2 ) and I L is not significantly dependent on p (H 2 O).
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