BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Xuehui Pang,Jihong Pan,Picheng Gao,Youying Wang,Liguo Wang,Bin Du,Qin Wei
It was reported that Proprotein convertase subtilisin/kexin type 6 (PCSK6) can promote the progression of rheumatoid arthritis to a higher aggressive status. In this work, a novel visible light induced photoelectrochemical (PEC) platform was designed to detect PCSK6 gene. ZnO@CdTe nanocable arrays/carboxylated g-C 3 N 4 used as the PEC signal generator . Hexagonal ZnO nanorods grew on ITO electrode firstly. CdTe were then electrodeposited on the ZnO nanorods surface to enhance the photogenerated h + /e − separation efficiency. Carboxylated g-C 3 N 4 was utilized to improve h + /e − separation efficiency and anchor the capture probes of PCSK6 gene by the covalent bonding effect. The 5′ and 3′ primers captured PCSK6 ssDNA by the specific recognition. The linear range was 10 pg/mL to 20.0 ng/mL with a detection limit of 2 pg/mL.
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CHEMICAL ENGINEERING COMMUNICATIONS
·
2016
·
IF 1.433
Lu Bai,Shu-Ni Li,Quan-Guo Zhai,Yu-Cheng Jiang,Man-Cheng Hu
In the present work, physicochemical properties, such as density (ρ), refractive index (nD) and viscosity (η), for six binary systems containing [Cnmim]Cl (Cnmim = 1-alkyl-3-methylimidazolium; n = 6, 8) and three dipolar aprotic solvents [N-methyl-2-pyrrolidinone, 1,3-dimethyl-2-imidazolidinone and 1,3-dimethyl-3,4,5,6-tetrahydro-2(1H)-pyrimidinone)] were measured at wide composition ranges and temperatures from T = 288.15 to 333.15 K at atmosphere pressure. Moreover, the derived properties, such as excess molar volumes (), apparent molar volumes (Vφ,i), deviations in refractive index (ΔnD), and isobaric thermal expansivity coefficients (αP), were calculated. The Redlich–Kister polynomial equation was fitted to the excess molar volumes and the deviation in refractive index data. The experimental results in this work allow us to discuss the interactions in the mixtures. The physicochemical properties of such systems may be useful for applications of imidazolium-based ionic liquids in the field of chemical engineering.
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CHEMISTRY OF MATERIALS
·
2024
·
IF 7.2
Yan Guo,Bin-Wen Liu,Chao-Hong Xie,Wen-Fa Chen,Ming-Xin Li,Xiao-Ming Jiang,Guo-Cong Guo
Rare-earth infrared nonlinear optical (IR NLO) compounds have garnered significant attention due to their abundant electronic structures. However, their NLO efficiency has been relatively modest, attributed to the localization of valence electron shells in rare-earth metals. Traditional design strategies for IR NLO materials have predominantly focused on MQ4 (M = metal, Q = S or Se) tetrahedra, with limited exploration of MQ6 octahedra. We introduce a novel approach to enhance NLO effects by incorporating distorted mixed anionic octahedral M(S/X)6 groups. To illustrate this strategy, we undertake structure modification and optimization of IR NLO performance using the Y–Ga–Ge–S system as an example. The synthesis of a new rare-earth chalcogenide, Y6Ga2GeS14 (1), serves as a foundation. Further halogen atom substitution yields Y6Ga4.2S13.6Cl3.4 (2), Y6Ga2.83Ge2S16.75Cl (3), and Y6Ga2.83Ge2S16.5Br1.5 (4). All compounds demonstrate remarkable NLO performance, including moderate NLO coefficients (0.17–0.70 × AgGaS2), large laser-induced damage thresholds (LIDTs) (3.78–6.19 × AgGaS2), and a broader transmission range (0.5–14.9 μm). The incremental enhancement of NLO efficiency in compounds 1–4 can be attributed to symmetry breaking induced by halogen atom substitution and increased distortion of the octahedral M(S/X)6 motifs in their structures, validating the effectiveness of our strategy. Notably, this strategy concurrently increases the band gap and LIDT, overcoming the NLO–LIDT incompatibility, being strikingly different from other reported approaches. This work provides new insights into optimizing the NLO performance of rare-earth IR NLO compounds through the modulation of octahedral motifs.
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BioScience Trends
·
2018
·Vol. 12
·期 2
·201-207
·
IF 1.844
Guiyan Yuan,Rui Zhang,Xuwang Chen,Benjie Wang,Ruichen Guo
An economical, rapid, and sensitive method of gas chromatography-mass spectrometry (GC-MS) was developed and validated to determine the presence of six pesticides (dichlorvos, acetochlor, atrazine, chlorpyrifos, α-endosulfan, and β-endosulfan) in human plasma. The pesticides were extracted with acetonitrile and concentrated using anhydrous sodium sulfate. Then, the target compounds were analyzed and quantified with GC-MS using borneol as an internal standard. Separation was performed on a HP-5MS capillary column (30 m × 0.25 mm × 0.25 µm) with temperature programming. Detection was accomplished under electro-spray ionization (ESI) in selected ion monitoring (SIM) mode. Under optimized conditions, satisfactory linear ranges of 0.05-10 μg/mL were obtained for all of the analyzed pesticides. The linear correlation coefficients were greater than 0.99. The average recovery was between 86.8 and 106.5%. The inter- and intra-day precision ranged from 1.7-14.5% and 4.2-13.8%, respectively. Dichlorvos was unstable in plasma both at room temperature and when frozen. The other five pesticides were stable after storage at – 20°C for 17 days and two freeze-thaw cycles. Thirty-five plasma samples from 15 patients with acute self-poisoning were analyzed using this method. Dichlorvos was found in 13 plasma samples with a mean concentration of 0.289 μg/mL, and atrazine was found in 6 with a mean concentration of 0.261 μg/mL. Acetochlor was found in one plasma sample (0.153 μg/mL). This method is simple, reliable and cost-effective. It takes little time and does not waste solvents, and it can be used to routinely detect six pesticides in patients with acute poisoning.
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JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2023
·
IF 2.6
Lingli Zhang,Renjie Xu
The mole fraction of 3-amino-6-bromopyridazine in methanol, ethanol, n-propanol, isopropanol, n-butanol, acetone, toluene, propylene glycol (PG), N,N-dimethylformamide (DMF), and water and methanol/ethanol//PG/DMF + water was experimentally measured by the isothermal saturation method from 278.15 to 323.15 K under 101.2 kPa. The solubility range of 3-amino-6-bromopyridazine is from 2.574 × 10–5 (water, 278.15 K) to 2.415 × 10–2 (DMF, 323.15 K). Accordingly, under the given conditions, the mixing of DMF and water is superior to other combinations in terms of dissolution. The solubility of 3-amino-6-bromopyridazine in mono-solvents was correlated with Apelblat, van’t Hoff, and λh models. Jouyban–Acree (J-A), modified Apelblat–Jouyban–Acree (A-J-A), and van’t Hoff–Jouyban–Acree (V-J-A) models were applied to calculate the solubility of 3-amino-6-bromopyridazine in four solvent mixtures. In terms of thermodynamic properties, the fusion enthalpy (ΔfusH = 37.01 kJ mol–1) and melting point (Tm = 472.15 K) of 3-amino-6-bromopyridazine are obtained by a differential scanning calorimeter. The interaction between the solute and solvent molecules as well as the interaction generated inside the above molecules could be analyzed through the calculation of the extended Hildebrand solubility parameter approach. The results showed that hydrophobic action of intramolecular groups and self-association of solute and solvent occurred in different concentration ranges.
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CARBOHYDRATE POLYMERS
·
2023
·
IF 11.2
Yazhen Wu,Xiaoxiao Li,Zhengyu Jin,Birte Svensson,Yuxiang Bai
How to improve the solubility of linear dextrins (LD) and retain their characteristic helix amphiphilic cavities with flexible embedding capability, is a question worth exploring without adding new chemical groups. The strategy presented in this study is to attach a highly flexible (α-1 → 6) glucochain at the reducing end of LD by preparing a new type of dextrin, referred to as single-arm linear dextrin (SLD). In the actual synthesis, an (α-1 → 6) linked oligosaccharide of DP ¯ 10.7 (PDI = 1.28) was formed by extension of glucose units onto sucrose (2 M) by using L940W mutant of the glucansucrase GTF180-ΔN firstly. Next using γ-CD as glucosylation donor γ-CGTase extended this (α-1 → 6) glucochain with (α-1 → 4) bonds. SLD is a chimeric glucosaccharide comprising an (α-1 → 4) linked part ( DP ¯ 10.5) attached to the nonreducing end of an (α-1 → 6) glucochain as verified by enzyme fingerprinting and 1 H NMR. Furthermore, SLD was validated to show greatly improved solubility and dispersibility of resveratrol in water, as indicated by a 3.12-fold enhancement over the solubility in the presence of 0.014 M SLD. This study provided a new strategy for solving the solubility problem of LD and opens possibilities for new design of the fine structure of starch-like materials.
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ELECTROPHORESIS
·
2022
·Vol. 43
·期 11
·1148-1160
·
IF 3.595
Yaqi Yao,Shanshan Wang,Rui Zhou,Ye Shang,Kunze Du,Jun He,Jin Li,Lin Ma,Yanxu Chang
A novel method of reverse migration micellar electrokinetic chromatography (RM-MEKC) using 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate) as oxidation-free radical was developed to screen the major antioxidants from Sanyetangzhiqing (SYTZQ), which is a new patent drug for diabetes. For simultaneous detection and separation of 2,2′-bis (3-ethylbenzothiazoline-6-sulfonic acid) ammonium salt (ABTS + ) and chemical components of SYTZQ, the key factors were optimized and the method was validated under the optimal conditions. All the relative standard deviations of recovery, precision, and stability were below 6.6%. Based on these, the RM-MEKC method has been successfully used to screen the main antioxidants from SYTZQ tablets. Five compounds, including rosmarinic acid, salvianolic acid B, lithospermic acid, hyperoside, and isoquercetin, were separated and identified as the major antioxidants. There was a linear relationship with correlation coefficient of 0.923 between the total amount of major antioxidants and total antioxidative activity of SYTZQ. Consequently, these five ingredients could be selected as combinatorial markers for quality control of SYTZQ, and the established method was concluded to be a simple, reliable, and powerful tool to screen and quantify active compounds for quality evaluation of SYTZQ.
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EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
·
2019
·
IF 4.833
Wei Zhao,Long He,Tian-Le Xiang,Ya-Jie Tang
The colchicine binding site of tubulin is an attractive molecular target domain for cancer therapies. However, there was no FDA approved drug for targeting colchicine domain. Our previous crystallography discovered that a potential binding site of αT5 loop-αH7 nearby colchicine domain was beneficial for introducing affinity fragment. In this work, benzo heterocycles (i.e., indole , indazole and quinoline) with the high affinity ability of αT5 loop-αH7 were chosen as affinity fragment to modify the molecule structure of podophyllotoxin for improving the tubulin binding affinity . 4β- NH -(benzo heterocycles)-4-desoxy-podophyllotoxin were synchronously located at α/β interface of tubulin through providing affinity fragment to αT5 loop-αH7 (i.e., α178Ser, α182Val, α241Phe) and colchicine domain (i.e., β241Cys, β124ASP). 4β- NH -(6″-aminoindole)-4-desoxy-podophyllotoxin not only exhibited nanomolar antitumor potency in vitro but also destroyed solid tumor growth without lethal toxicity in vivo . The correctness of rational drug design was strictly demonstrated by bioactivity test.
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JOURNAL OF SEPARATION SCIENCE
·
2019
·Vol. 42
·期 15
·2482-2490
·
IF 2.516
Lingping Cheng,Jianfeng Cai,Qing Fu,Yanxiong Ke
6-(4-Aminophenyl)-5-methyl-4,5-dihydro-3(2H)-pyridazinone is a key synthetic intermediate for cardiotonic agent levosimendan. Very few studies address the use of chiral stationary phases in chromatography for the enantioseparation of this intermediate. This study presents two efficient preparative methods for the isolation of ( R )(−)-6-(4-aminophenyl)-5-methyl-4,5-dihydro-3(2H)-pyridazinone in polar organic solvent chromatography and supercritical fluid chromatography using polysaccharide-based chiral stationary phases and volatile organic mobile phases without additives in isocratic mode. Under optimum conditions, Chiralcel OJ column showed the best performance ( α = 1.71, Rs = 5.47) in polar organic solvent chromatography, while Chiralpak AS column exhibited remarkable separations ( α = 1.81 and Rs = 6.51) in supercritical fluid chromatography with an opposite enantiomer elution order. Considering the sample solubility, runtime and solvent cost, the preparations were carried out on Chiralcel OJ column and Chiralpak AS column (250 × 20 mm i.d.; 10 µm) in polar organic mode and supercritical fluid chromatography mode with methanol and CO 2 /methanol as mobile phases, respectively. By utilizing the advantages of chromatographic techniques and polysaccharide-based chiral stationary phases, this work provides two methods for the fast and economic preparation of ( R )(−)-6-(4-aminophenyl)-5-methyl-4,5-dihydro-3(2H)-pyridazinone, which are suitable for the pharmaceutical industry.
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TOXICOLOGY
·
2025
·
IF 4.6
Han Gao,Hanyu Gao,Hao Yin
Emodepside, a novel antiparasitic agent, exhibits remarkable efficacy against nematodes. Recent research from a phase 2b clinical trial has demonstrated that the most frequent adverse event in the emodepside treatment group is blurred vision, but the mechanism of blurred vision remains largely unexplored. To provide references for secure medication and prospective therapeutic interventions of emodepside, we conducted an investigation into the cytotoxic effects of emodepside on the corneal stroma and its underlying mechanisms using an in vitro model of human corneal stromal (HCS) cells. The loading of the mini-chromosome maintenance 6 protein (MCM6) onto chromatin at replication origins by chromatin licensing and DNA replication factor 1 (CDT1) constitutes a crucial step in licensing DNA for replication. This study demonstrates that emodepside directly binds to the CDT1-binding domain (CBD) of MCM6, competitively blocking the MCM6-CDT1 interaction as confirmed by modified enzyme-linked immunosorbent assay and drug affinity responsive target stability assays. Molecular docking reveals that emodepside binds to CBD through hydrogen bonds with Lys754, Ile760, and Lys770. In HCS cells, this blockade results in the inhibition of DNA replication licensing and a decrease in DNA synthesis, as evidenced by the ethynyl-deoxyuridine incorporation assay, leading to cell cycle arrest at the G 0 /G 1 phase. Moreover, emodepside-induced cell cycle arrest in HCS cells leads to dose-dependent apoptosis and proliferation inhibition with the IC 50 value being 32.3 μM. In conclusion, this study not only provides new insights into the mechanism of emodepside-induced vision blur but also offers a valuable molecular tool for the research of MCM6-CDT1 interaction.
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JOURNAL OF SOLID STATE CHEMISTRY
·
2021
·
IF 3.498
Mei Yan,Wen-Dong Yao,Wenfeng Zhou,Wenlong Liu,Ru-Ling Tang
Inorganic gallates are attractive owing to their abundant structures and wide applications for nonlinear optics, magnetism , molecular sieve and catalysis. Herein, a new cesium cadmium gallate, CsCdGa 3 O 6 , has been synthesized by a solid-state reaction, which is the first I-II-III-O (I = alkali metals, II = divalent metals, III = Al, Ga, In) type oxide. It crystallizes in the centrosymmetric space group P 2 1 / c , and the three-dimensional Ga–O polyanionic framework is constructed by [Ga 4 O 14 ] 16− helical belts linked [Ga 6 O 6 ] 6+ and [Ga 8 O 8 ] 8+ cages. Thermal analysis indicates that it possesses high thermal stability. Various characterizations and results analysis including XRD , TG-DSC, IR, Raman spectrum , as well as electronic structure calculations were performed.
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Foods
·
2025
·Vol. 14
·期 8
·1292
·
IF 5.1
Chunxiao Li,Xiaolei Zhao,Xiaofei Yin,Shuting Zhang,Jinxing He
2-amino-1-methyl-6-phenyl-imidazo [4,5-b] pyridine (PhIP) is a harmful compound that is formed during the high-temperature processing of meat products, and the risk of cancer may be increased with the prolonged intake of foods containing high levels of PhIP. This study aimed to develop an innovative detection method specifically for PhIP in meat products. Utilizing hydrogel-molecular-imprinting electrochemical sensing technology, the preparation conditions of molecularly imprinted hydrogels (MIHs) were optimized. Consequently, a highly selective and rapid detection method for PhIP was successfully established, integrated with an electrochemical workstation. The results indicate that the prepared MIHs exhibit an excellent specific recognition performance for PhIP. The sensor exhibited a linear response within the concentration range of 1.0–200.0 ng/mL, with a detection limit of 0.07 ng/mL (S/N = 3) under optimized conditions. In addition, the accuracy and reliability of the method were verified by spiked recovery experiments, and the recoveries ranged from 75.9% to 108.8%, which demonstrated its high accuracy and potential for practical application.
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