查找使用阿拉丁产品的科研文献

Chemiluminescence of luminol enhanced by self-circulation heterogeneous Fenton reaction on the surface of CoFe2O4·MoS2 nanosheets

Zhiqiang Wang,Zhi Wang,Jing Wu

Exploring advanced materials to enhance chemiluminescence (CL) emission is an original approach and sheds light on improving sensitivity of CL analysis. Fenton material and its relative reactions are scarcely utilized in CL systems although they are commonly applied in environmental monitoring, biological detection, chemical analysis and life science researches. In this study, one kind of Fenton material CoFe 2 O 4 ·MoS 2 nanosheets were successfully synthesized and applied in enhancing the CL intensity of the luminol-H 2 O 2 system. An acidic microenvironment was constructed on the surface of CoFe 2 O 4 ·MoS 2 nanosheets and ensured a stable circulation of iron ions which were beneficial for reactive oxygen species generation and then dramatically enhanced the CL emission of luminol-H 2 O 2 system even in alkaline conditions. This work introduces a new approach to enhance traditional CL process and explores new application fields of heterogeneous Fenton catalase-like catalysts.

Co3O4/NiCo2O4 heterojunction as oxygen evolution reaction catalyst for efficient luminol anode electrochemiluminescence

Chulei Zhao,Chaoyun Ma,Fuping Zhang,Wenjun Li,Chenglin Hong,Fuxi Bao

Luminol has garnered significant attention from analysts as one of the most effective and commonly used electrochemiluminescence (ECL) reagents. However, the efficient luminescence of luminol anode is limited by the excitation of various reactive oxygen species (ROS). Typically, ROS are generated through co-reactive reagents and dissolved oxygen. Unfortunately, the former suffers from two drawbacks, namely biotoxicity and instability, while the latter cannot offer sufficient oxygen due to its limited solubility in aqueous solutions. Consequently, a low decomposition rate is usually obtained, leading to insufficient ROS. Therefore, there is an urgent need to develop efficient luminol anode systems. This study focuses on the use of zeolitic imidazolate framework-67 (ZIF-67) as a template, employing a controlled chemical etching method to create a ZIF-67/Ni-Co-layered double hydroxide (LDH). The intermediate composite is then annealed in air, resulting in the formation of a Co 3 O 4 /NiCo 2 O 4 double-shelled nanobox (DSNB) heterostructure . Due to its structural advantages, the DSNB exhibits excellent electrocatalytic performance in the oxygen evolution reaction (OER). Furthermore, it was found that both the intermediates and products of OER can directly participate in the luminol chemiluminescence process, ultimately resulting in a 700-fold increase in the electrochemiluminescence (ECL) signal compared to an equal molar concentration of luminol solution. This work not only establishes the OER-mediated ECL system but also deepens the understanding of the relationship between ROS and luminol, providing a new pathway to study the luminol anodic ECL luminescence system.

Aptamer cleavage optimization, binding mechanism analysis, and dual-chemiluminescence sensor for microcystin detection

Zhongmei Peng,Xuning Kang,Zhenlin Fan,Jingping Zhang,Yuzhu Song,Jinyang Zhang,Qinqin Han

This study aimed to develop a high-affinity and highly specific aptamer, microcystins (MCs)-12 using Capture-Systematic Evolution of Ligands by Exponential Enrichment technology based on immobilized libraries. This aptamer simultaneously recognized three conformations of microcystins (MC-LR, MC-RR, and MC-YR). The stem-loop structure of the aptamer sequence MCs-12 was cleaved and optimized by removing the bases step by step. The optimized sequence M-Apt-6, which had an affinity for MCs, was obtained with only 27 bases. In addition, the possible binding sites and mechanisms of M-Apt-6 for MC-LR, MC-RR, and MC-YR were predicted by circular dichroism, molecular docking, and molecular dynamics simulations. A chemiluminescence sensor was developed based on M-Apt-6 to detect MC-LR, MC-RR, and MC-YR in Dianchi Lake water simultaneously. The M-Apt-6 aptamer specifically bound to MCs. The aptamer not bound to MCs was adsorbed on AuNPs to produce a testable chemiluminescent signal in a luminol-AgNO 3 or luminol-H 2 O 2 system. The aggregation of AuNPs induced by the MCs/M-Apt-6 complex catalyzed the luminol-AgNO 3 or luminol-H 2 O 2 chemiluminescence reactions. The results showed a significant linear relationship between chemiluminescence intensity and MCs concentration. The luminol-AgNO 3 and luminol-H 2 O 2 systems showed good linearity for MCs concentration in the range of 0.5–25.0 ng/mL and 0.5–100.0 ng/mL, with the detection limits of 0.22 ng/mL and 0.01 ng/mL, respectively.

Neohesperidin Dihydrochalcone versus CCl4-Induced Hepatic Injury through Different Mechanisms: The Implication of Free Radical Scavenging and Nrf2 Activation

Chuanyang Su,Xiaomin Xia,Qiong Shi,Xiufang Song,Juanli Fu,Congxue Xiao,Hongjun Chen,Bin Lu,Zhiyin Sun,Shanmei Wu,Siyu Yang,Xuegang Li,Xiaoli Ye,Erqun Song,Yang Song

Neohesperidin dihydrochalcone (NHDC), a sweetener derived from citrus, belongs to the family of bycyclic flavonoids dihydrochalcones. NHDC has been reported to act against CCl4-induced hepatic injury, but its mechanism is still unclear. We first discovered that NHDC showed a strong ability to scavenge free radicals. In addition, NHDC induces the phase II antioxidant enzymes heme oxygenase 1 (HO-1) and NAD(P)H/quinone oxidoreductase 1 (NQO1) through the activation of the nuclear factor (erythroid-derived 2)-like 2 (Nrf2)/antioxidant response element (ARE) signaling. Further assays demonstrated that NHDC induces accumulation of Nrf2 in the nucleus and augmented Nrf2-ARE binding activity. Moreover, NHDC inhibits the ubiquitination of Nrf2 and suggests the modification of Kelch-like ECH-associated protein 1 (Keap1) and the disruption of the Keap1/Nrf2 complex. c-Jun N-terminal kinase (JNK) and p38 but not extracellular signal-regulated protein kinase (ERK) phosphorylations were up-regulated by NHDC treatment. Taken together, NHDC showed its protective antioxidant effect against CCl4-induced oxidative damage via the direct free radical scavenging and indirect Nrf2/ARE signaling pathway.

Mismatched catalytic hairpin assembly coupling hydroxylamine-O-sulfonic acid as oxide for DNA assay

Xu Hun,Yan Meng,Shanshan Wang,Hui Zhang,Xiliang Luo

Catalytic hairpin assembly (CHA) is an enzyme-free amplification method that it has been proved to be useful in signal amplification. In this work, hydroxylamine- O -sulfonic acid (HOSA) is used as an oxide for LumAuNPs chemiluminescence (CL) in mismatched catalytic hairpin assembly (MCHA) for the first time. Functionally, this system consists of two hairpin species, the biotin-labeled hairpin H1 and LumAuNPs-labeled hairpin H2, which are stable and unable to hybridize in the absence of target DNA. However, the MCHA is initiated in the presence of target DNA, which regenerates the target DNA to catalyze another new cyclic reuse and leads to the formation of numerous H1-H2 complexes. In this case, HOSA serves as an oxidizing agent reacted with LumAuNPs, which can generate strong CL. In this fascinating hairpin assembly amplification strategy, mismatched base sequences are designed in hairpin H2 to decrease nonspecific CHA products, which reduce the background signal significantly. Under the optimal experimental conditions, this approach shows high selectivity even toward single-base mismatch and improves sensitivity greatly for target DNA with a linear range of 2.5 fM–1 pM, achieving a detection limit as low as 0.8 fM. The developed method holds a great promise for further applications in early clinical diagnosis and therapy.

An ultrasensitive chemiluminescence sensor for sub-nanomolar detection of manganese(II) ions in mineral water using modified gold nanoparticles

Qi Li,Yi He

A simple, rapid, facile and ultrasensitive chemiluminescence (CL) sensor was developed for detection of Mn 2+ in mineral water samples. The developed CL sensor was based on the enhancement effect of manganese ion (Mn 2+ ) on the CL between luminol-modified AuNPs and AgNO 3 system. In the absence of Mn 2+ , the CL intensity from the luminol-modified AuNPs and AgNO 3 was relative weak owing to the bad stability of superoxide anion radical (O 2 − ) which was the key intermediate for the CL reaction. However, the addition of Mn 2+ to this CL system would generate hydrogen peroxide (H 2 O 2 ) according to the specific reaction of Mn 2+ and O 2 − . The produced H 2 O 2 had a better stability and reaction activity, thereby enhancing the CL emission. This phenomenon allowed to selective and highly sensitive detection of Mn 2+ . Under the optimal sensing conditions, the developed CL sensor showed a wide linear range for Mn 2+ detection in the range of 1–600   nM, with a detection limit of 0.3   nM at a signal-to-noise of 3. This sensitivity was about 2–3 orders of magnitude higher than that reported techniques based on metal nanoparticles-based optical sensors . In addition, the developed CL sensor exhibited excellent selectivity toward Mn 2+ over other metal ions , and it had a good stability and reproducibility.

A chemiluminescence metalloimmunoassay for sensitive detection of alpha-fetoprotein in human serum using Fe-MIL-88B-NH2 as a label

Jingrong Zhou,Zhou Long,Yunfei Tian,Xiaodong Ding,Lan Wu,Xiandeng Hou

Electrochemiluminescence bipolar electrode array for the multiplexed detection of glucose, lactate and choline based on a versatile enzymatic approach

Yi Xiao,Linru Xu,Lian-Wen Qi

A simple, efficient and versatile biosensing platform capable of the multiplexed detection for glucose, lactate and choline was developed by the integration of bipolar electrochemistry and electrochemiluminescence (ECL) imaging. The sensing bipolar electrodes (BPEs) were simply modified via a one-step method adaptable to different enzymes. The biorecognition event happening between the substrate and the corresponding enzyme could be directly reported by the ECL emitted on the same pole from luminol and in situ generated H 2 O 2 . Under optimized conditions, the BPEs array was successfully applied for the determination of glucose, lactate and choline in the ranges of 0.01–1   mM, 0.01–1   mM and 0.02–5   mM, with the LOD of 7.57   μM, 8.25   μM and 43.19   μM, respectively. Owing to the improved stability of in situ generated H 2 O 2 , a whole series of analytes testing could be completed in the same BPE biochip. Subsequently, an array chip consisting of nine BPEs enabled the concomitant detection of glucose, lactate and choline, demonstrating the capability for multifunctional detection of biomolecules. This versatile analytical system could be easily extended to sensitive screening in a miniaturized device and point of care testing.

Highly luminescent S,N co-doped carbon quantum dots-sensitized chemiluminescence on luminol–H2O2 system for the determination of ranitidine

Jianqiu Chen,Juan Shu,Jiao Chen,Zhiran Cao,An Xiao,Zhengyu Yan

S,N co-doped carbon quantum dots (N,S-CQDs) with super high quantum yield (79%) were prepared by the hydrothermal method and characterized by transmission electron microscopy, photoluminescence, UV–Vis spectroscopy and Fourier transformed infrared spectroscopy. N,S-CQDs can enhance the chemiluminescence intensity of a luminol–H 2 O 2 system. The possible mechanism of the luminol–H 2 O 2 –(N,S-CQDs) was illustrated by using chemiluminescence, photoluminescence and ultraviolet analysis. Ranitidine can quench the chemiluminescence intensity of a luminol–H 2 O 2 –N,S-CQDs system. So, a novel flow-injection chemiluminescence method was designed to determine ranitidine within a linear range of 0.5–50 μg ml −1 and a detection limit of 0.12 μg ml −1 . The method shows promising application prospects.

Selective chemiluminescent sensor for detection of mercury(II) ions using non-aggregated luminol-capped gold nanoparticles

Qi Li,Jiahua Wang,Yi He

A selective chemiluminescent sensor was developed for the detection of mercury(II) ions (Hg 2+ ) in environmental water and soil supernatant samples using non-aggregated luminol-capped gold nanoparticles (AuNPs). Luminol-capped AuNPs can react with silver nitrate (AgNO 3 ) under alkaline environment , generating a strong chemiluminescence (CL) emission because AuNPs had excellent catalytic activity for luminol-AgNO 3 CL system. However, when Hg 2+ was added to the solution of luminol-capped AuNPs, it would be adsorbed on the surface of luminol-capped AuNPs according to the inherent affinity of AuNPs for Hg 2+ . The presence of Hg 2+ significantly inhibited the catalytic activity of AuNPs, leading to CL quenching. With the increasing of Hg 2+ concentration, the CL intensity gradually decreased. Under the optimal detection conditions, the developed CL sensor showed a good linear relationship in range of 10–600   nM with a limit of detection of 1   nM at a signal-to-noise ratio of three which was below the maximum allowable concentration for Hg 2+ in drinking water (10   nM) defined by United States Environmental Protection Agency. Also, the developed CL sensor exhibited high selectivity toward Hg 2+ over other environmentally relevant metal ions at high concentrations (500   μM).

A portable chemiluminescence imaging immunoassay for simultaneous detection of different isoforms of prostate specific antigen in serum

Anran Liu,Fang Zhao,Yuewu Zhao,Li Shangguan,Songqin Liu

A multianalyte chemiluminescence (CL) imaging immunoassay strategy for sensitive detection of different isoforms of prostate specific antigen (PSA) was developed. The microtiter plates were fabricated by simultaneously immobilizing of free-PSA (f-PSA) and total-PSA (t-PSA) capture antibody on nitrocellulose (NC) membrane. Each of the array were spotted in replicates of six spots within a spacing of 2   mm. 16 or 48 detection wells were integrated on a single NC membrane and each well could be used as a microreactor and microanalysis chamber. Under a sandwiched immunoassay, the CL signals on each sensing site were collected by a charge-coupled device (CCD), presenting an array-based chemiluminescence imaging. Soybean peroxidase (SBP) was used to label f-PSA or t-PSA monoclonal antibody . With the amplification effects of two enhancers, 3-(10′-phenothiazinyl) propane-1-sulfonate (SPTZ) and 4-morpholinopyridine (MORP), the CL intensity could significantly enhanced, which improved the sensing sensitivity and detection limit. Under the optimal conditions, the linear response to the analyte concentration ranged from 0.01–36.7   ng/mL and 0.02–125   ng/mL for f-PSA and t-PSA, respectively. The results for the detection of forty serum samples from prostate cancer patients and cancer-free patients showed good agreement with the clinical data, suggesting that the proposed assay had acceptable accuracy. The proposed CL imaging immunoassay possess high throughput and acceptable reproducibility, stability and accuracy, which made it great potential to available to distinguish different isoforms of PSA in serum samples.

Combining complement fixation and luminol chemiluminescence for ultrasensitive detection of avian influenza A rH7N9

Man Li,ZhuanZhuan Shi,ChangMing Li,Ling Yu

The complement fixation test (CFT) is a serological test that can be used to detect the presence of either a specific antibody or antigen to diagnose infections. In a conventional CFT, the assay result is determined by observing the clarity of the reaction solution or the sediment of red cells by the naked eye. Although the assay conditions are thereafter simplified, the sensitivity of the assay would be sacrificed due to the limitation of bulk observation. Inspired by the forensic scientists to examine blood at the scene of the crime, we rationally argued that the luminol chemiluminescence (CL) reaction could be applied in the CFT to sense physiological complement-mediated haemolytic phenomena for sensitive protein detection. The combination of the CFT and the luminol CL system was demonstrated in detection of rH7N9, a recombinant avian influenza virus protein. The testing can be accomplished within 2.5 h and the linear detection range covers 0.25 fg mL−1 to 25 ng mL−1. The feasibility of the CL based CFT in assaying a real biopsy was successfully demonstrated by specifically detecting rH7N9 and the carcinoembryonic antigen (CEA) in human serum. This new type of protein detection approach inherits the beauty of complement-mediated assay, such as being fast, and no protein immobilization, blocking and washing. In addition, the participation of luminol CL enables us to quantitatively analyse the intensity of a haemeolysis process, ameliorating the limitation of bulk observation in traditional CFT. It is anticipated that the luminol CL-CFT assay would be particularly suitable for investigation of small molecules, toxins, and short peptides.