Foods
·
2022
·Vol. 11
·期 19
·3130
·
IF 5.561
Juanli Du,Hao Wu,Xu Jing,Yonghe Yu,Zhisheng Yan,Jianhai Zhang
Developing a simple and effective method for measuring carbaryl residues in food is urgent due to its widespread use and the associated health risks in agriculture, as well as various defects in existing detection techniques. The COF (TpPa-NH2)@Fe3O4nanocomposite (amino modification) was synthesized via a two-step method and used as an adsorbent for the extraction of carbaryl from food samples in this study. The results indicated that COF (TpPa-NH2)@Fe3O4can rapidly and successfully capture carbaryl directly from samples via π–π stacking and hydrophobic interactions, achieving maximum adsorption within 5 min under a small adsorbent quantity using a fluorescence spectrophotometer. Under the optimized conditions, carbaryl exhibited good linearity in the range of 0.2–120 µg·kg−1, and the limit of detection was 0.012 µg·kg−1. The recoveries of the samples were 96.0–107.4%. This method has broad application prospects for the monitoring of carbaryl in food.Keywords:magnetic solid-phase extraction;COF (TpPa-NH2)@Fe3O4nanocomposite;fluorescence spectroscopy;carbaryl;food samples
研究领域:
食品安全
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分析化学
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Jutao Hu,Xiaoyu Su,Lei Yuan,Kaiyi Zheng,Xiaobo Zou,Zongbao Sun,Xuechao Xu,Wen Zhang
Accurate and sensitive detection of chloramphenicol (CAP) in natural samples is essential for ensuring human health. Herein, an enzyme-regulated fluorescence sensor using Fe 3 O 4 @COF/Fe 3+ probe, is developed for CAP determination. Fe 3 O 4 @COF, synthesized via hydrothermal method , exhibits dual functions as a magnetic carrier and signal probe. Bovine serum albumin conjugated-chloramphenicol, adsorbed on the surface of Fe 3 O 4 @COF, competes with CAP for antibody binding. The antibody interacts with alkaline phosphatase via the biotin-streptavidin system. Meanwhile, ascorbic acid , produced from the enzyme-catalyzed reaction dominated by alkaline phosphatase, effectively restores the fluorescence of Fe 3 O 4 @COF that is quenched by Fe 3+ . After experimental verification and gradual optimization, a logarithmic linear relationship between CAP concentration and fluorescence intensity is established in the range of 2 × 10 −4 ∼10 μg mL −1 , with a good limit of detection (9.2 × 10 −5 μg mL −1 ). Proposed method exhibits excellent stability (15 days) and reusability (8 cycles), providing a sensitive and reliable method for accurate CAP detection. The readouts show good agreement with HPLC and recoveries during laboratory and natural CAP analysis.
研究领域:
分析化学
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生物化学
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环境科学
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食品安全
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Ruiwen Li,Heng Fan,Hao Shen,Shaoning Yu
Given the frequent occurrence of multidrug-resistant bacteria (MDRB), which pose a severe threat towards public hygiene, it is important to construct facile and sensitive strategies for the detection of MDRB. We developed a fluorescent/colorimetric dual-mode biosensing platform for the analysis of MDRB by integrating the remarkable peroxidase-like activity of boric acid-decorated fluorescent framework hybrid (MOF@COF) nanozyme and enhanced bacterial affinity of DNA programmable multivalent aptamer scaffold. The efficiency and sensitivity of the proposed analysis approach were dramatically increased due to richer aptamers and high-performance MOF@COF nanozyme. Compared with commonly used single recognition methods, multivalent aptamers and bacteria-affinity boric acid groups on the nanozyme surface could identify multiple sites of MDRBs to improve site utilization for ultrasensitive biosensing. The platform realized selective detection of Acinetobacter baumannii (AB) and Pseudomonas aeruginosa (PA) in two modes with consistent results. In colorimetry and fluorescence modes, the linear ranges of AB were 10 2 -10 8 and 10–10 8 CFU/mL with corresponding detection limits of 12 and 2 CFU/mL, and the linear ranges of PA were 10 2 -10 8 and 10–10 8 CFU/mL with corresponding detection limits of 10 and 3 CFU/mL, respectively. More importantly, the biosensing platform could be applied for MDRB quantification in biological specimens, including human serum, cerebrospinal fluid, and urine with remarkable recoveries (96.8–105.0%) in both modes, indicating the bright prospect of the biosensing platform for MDRB determination in clinical diagnosis.
研究领域:
生物化学
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医学诊断
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纳米技术
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产品关联
DOI
高影响因子
Journal of Future Foods
·
2025
·
IF 7.2
Pengfei Dong,Shuang Wu,Youfa Wang,Ning Yu,Vijaya Raghavan,Jin Wang
Sesame allergy has emerged as a public health issue due to its persistent occurrence, severe symptomatic manifestations, and widespread incidence globally. Among sesame allergens, 2S albumins (Ses i 2) is one of the widely recognized allergens, and its precise detection is urgently needed to prevent sesame-related allergies. In this work, a composite structure was designed and synthesized by combining metal ion self-assembled metal organic frameworks (MOFs) and imine constructed covalent organic frameworks (COFs) (represented by MOF@COF), which constructing a high-sensitivity sensing system for identification and detection of Ses i 2. A novel hybrid material, comprising a strategically assembled combination of UIO-66-NH 2 and TA-COF, was crafted to yield a functionalized UIO-66-NH 2 @TA-COF composite, demonstrating remarkable electrochemical performance. As a result, a significant quantity of aptamer strands can adhere to the UIO-66-NH 2 @TA-COF surface resulting from robust π-π stacking interactions. With the change in the relative concentration of Ses i 2 from 5 to 650 nM, the UIO-66-NH 2 @TA-COF sensor exhibited a low detection limit of 0.13 nM, which demonstrates its extremely high sensitivity. In addition, the UIO-66-NH 2 @TA-COF sensor exhibited excellent reproducibility, stability and specific recognition ability to Ses i 2. Therefore, this innovative sensor exhibits great potential for real-world applications in food safety.
研究领域:
生物传感器
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食品科学
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纳米技术
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分析化学
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材料科学
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过敏研究
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产品关联
DOI
高影响因子
RSC Advances
·
2025
·Vol. 15
·期 11
·8111-8120
·
IF 4.6
Hao Zhang,Weihao Ma,Chunyu Qiang,Jiayuan Nie,Ling Ma,Yawei Zhang,Ke Wang
In our research, an emerging magnetic covalent organic framework (Fe3O4@SiO2–BD–DADB-COF) was formulated through Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), etc. Several parameters affecting the extraction process were refined. Accordingly, a novel method of determining 15 macrolides (MALs) in honey and water was established through the Fe3O4@SiO2–BD–DADB-COF as a magnetic solid-phase extraction (MSPE) adsorbent and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). In consequence, the standard curves for the 15 MALs exhibited an exceptional linearity from 0.1 to 200 μg L−1, and the correlation coefficients (R2) varied from 0.9990 to 0.9999. The recoveries fell between 70.01% and 115.56%, with the relative standard deviations (RSDs) being below 9.93% (n = 5). The detection limits reached 0.001–0.075 μg L−1 with the quantification limits being 0.004–0.228 μg L−1. Ultimately, the method was excellently applied to the analysis of MALs in honey and water.
研究领域:
分析化学
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环境科学
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食品科学
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产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2021
·
IF 6.707
Nan Zhang,Tao Bao,Yan Gao,Xianliang Xu,Sicen Wang
[email protected] composite was fabricated for greater adsorption capacity through the utilization of corncob, which is a natural, sustainable, and cellulose-rich resource. An in-situ growth strategy was implemented to graft UiO-66-NH 2 on carboxyl-terminal corncob induced by the thermal esterification of critic acid. Subsequently, [email protected] 2 @TpBD was constructed through the Schiff base reaction between 2,4,6-triformylphloroglucinol (Tp) and UiO-66-NH 2 as well as benzidine (BD). The ratio of UiO-66-NH 2 and TpBD was optimized to be 5:3 for enhancing adsorption capacity. Based on the π-π, hydrogen bonding, and -SO 2 -Zr-O- coordination effect, sulphonamides (SAs) were chosen to investigate the adsorption capacity of adsorbent using an in-syringe solid phase extraction (IS-SPE) method. The binding energy calculated using density functional theory (DFT) reached −37.45 Kcal/mol, which indicated the strong interactions between SAs and [email protected] 2 @TpBD. Combined with ultra-performance liquid chromatography (UPLC), a high sensitivity was obtained with low detection of 0.10 μg/L. The prepared [email protected] 2 @TpBD exhibited good reproducibility within relative standard deviations (RSDs) 3.1%-3.4% in intra-batch and 4.3%-8.4% in inter-batch. Moreover, the extraction capacity of adsorbent was not decreased in 20 recycles adsorption–desorption within RSDs 3.8%-8.5%. With features of permanent porosity, multiple active sites, and good renewability, [email protected] 2 @TpBD shows great potential towards industrial applications in the field of adsorption.
研究领域:
环境科学
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材料科学
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分析化学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Yingfei Hui,Yuli Wei,Hao Guo,Mingyue Wang,Liping Peng,Borong Ren,Yanyan Ma,Wu Yang
Glyphosate (Gly) overuse threatens public health and ecosystems, necessitating highly sensitive detection. This work developed a novel ratiometric fluorescent probe, CDs@Cu-MOF/TMTA-COF, by integrating carbon dots (CDs), metal organic frameworks (MOFs), and covalent organic frameworks (COFs). We encapsulated CDs within Cu-MOF in-situ grown onto sp 2 ‑carbon conjugated TMTA-COF. Gly binding to Cu 2+ causes MOF structural collapse, releasing CDs and enhancing its fluorescence at 425 nm. Simultaneously, Gly inhibits competitive absorption and acceptor-photoinduced electron transfer process between TMTA-COF and Cu-MOF, increasing COF fluorescence at 550 nm. This dual-signal change enables ratiometric Gly detection from 0.2 to 40 μM, with a detection limit of 0.07 μM. This probe was successfully used to measure Gly in soil, Yellow River water and soybean (recoveries: 95.3–104.1 %). Additionally, the back propagation neural network further enables fast detection of Gly. This work demonstrates the potential of MOF/COF hybrids for fluorescence sensing for pesticide analysis and paves the way for intelligent sensing platforms.
研究领域:
分析化学
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环境科学
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材料科学
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传感器技术
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纳米技术
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神经网络
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE CHINESE CHEMICAL SOCIETY
·
2020
·Vol. 67
·期 11
·2004-2011
·
IF 1.554
Na Zhang,Fei Wang,Chang-chen Cai,Qian Sun,Kai Zhang,Ai-xiang Li,Jun-ying Weng,Qiu-hong Li
Based on a facile and universal method using noncovalent bonding, here, polymer-modified covalent organic framework materials (COFs) were rapidly prepared. PNIPAM-modified COF (COF-PNIPAM) was synthesized by electrostatic self-assembly between COF prepared by low-cost melamine and terephthalaldehyde and carboxyl-terminated poly( N -isopropylacrylamide) (PNIPAM) prepared via reversible addition-fragmentation chain transfer polymerization. The carboxyl-terminated PNIPAM was characterized by Gel Permeation Chromatography and Ultraviolet and Visible Spectroscopy (UV–vis). COF and COF-PNIPAM were characterized by Fourier Transform Infrared Spectroscopes, Scanning Electron Microscope, Transmission Electron Microscope, X-Ray Diffractomer (XRD), and Brunner-Emmet-Teller (BET) measurements. The results showed that the COF-PNIPAM was successfully prepared. The Pickering emulsion properties of the COF-PNIPAM in a water-to-toluene mixed system were studied, and when the water-to-oil ratio is 2:1, a stable emulsion can be obtained. However, the results show that the PNIPAM chains of COF-PNIPAM nearly lose their temperature responsiveness.
研究领域:
材料科学
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化学工程
·
胶体与表面化学
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产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·Vol. 415
·期 27
·6771-6783
·
IF 4.3
Ma Wanwan,Chen Huan,Hou Hongwei,Hu Qingyuan,Bai Yu
Phosphatidylcholine (PC) and lysophosphatidylcholine (LPC), two types of phospholipids (PLs), have been reported to be closely correlated with head and neck cancers of laryngeal cancer (LC) and thyroid cancer (TC), which make their analysis crucial. TiO 2 @COF-based solid-phase microextraction (SPME) coupled to UHPLC-MS/MS was developed for the rapid and accurate detection of seven potential PL biomarkers from small amounts of serum in this work. The combination of TiO 2 and COF proves to be effective for the extraction of the target analytes. Under optimal conditions, the developed TiO 2 @COF-based SPME-UHPLC-MS/MS method revealed good linearity ( R 2 ≥ 0.997) with LODs ranging from 0.05 to 0.38 ng/mL for PLs, the extraction recoveries and matrix effects ranging from 83.09–112.03% and 85.38–113.67%, respectively. As a high-throughput pretreatment method, satisfactory probe-to-probe reproducibility rates of 2.7–10.1% were obtained. Finally, the TiO 2 @COF-based SPME-UHPLC-MS/MS method was applied to analyze LPC 14:0, LPC 16:0, LPC 18:0, LPC 18:1, LPC 19:0, PC 16:0/18:1, and PC 18:0 in serum samples from early LC patients ( n = 15), early TC patients ( n = 15), and healthy volunteers ( n = 15). The results indicated that cancer patients could be effectively differentiated from healthy controls using orthogonal partial least squares discriminant analysis (OPLS-DA). In conclusion, the established TiO 2 @COF-based SPME-UHPLC-MS/MS method is reliable for the rapid determination of the seven PLs in serum samples, which is promising for early diagnosis of head and neck cancers. Graphical abstract
研究领域:
分析化学
·
肿瘤学
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Peng Jin,Xiaoying Niu,Fang Zhang,Kui Dong,Hongxia Dai,Huige Zhang,Weifeng Wang,Hongli Chen,Xingguo Chen
Covalent organic frameworks (COFs), as one of the most significant members of the porous organic frameworks, have been well used in the photocatalysis owing to their outspread π-conjugated framework, high crystallinity and regular pore structure. Herein, after reducing the labile imine-linked COF-300 to the more stable amine-linked COF-300-AR, we for the first time demonstrated that COF-300-AR was the light-responsive oxidase mimic. COF-300-AR exhibited excellent oxidase-mimicking activity under purple light stimulation (λ = 400 nm), which can catalyze the oxidation of classical substrates such as 3,3′,5,5′-tetramethylbenzydine (TMB) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) by the formation of •OH and O2•– free radicals in the presence of dissolved oxygen. The COF-300-AR oxidase mimic has outstanding advantages of easy light control, high stability, good reusability, and highly catalytic oxidation capacity and has been applied to detect glutathione (GSH) levels in HL60 cells with good selectivity and high sensitivity. This study will broaden the sensing applications of COFs and offer a promising build block for the construction of artificial enzymes.
研究领域:
材料科学
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纳米技术
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分析化学
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生物化学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 11
·1-11
·
IF 5.3
Wei Yuxin,Fu Yang,Li Chuanhai,Chen Siyu,Xie Linzhi,Chen Mei
A electrochemical biosensor was designed utilizing a CRISPR Cas9n-driven DNA walker combined with gold-nanosphere-like covalent organic frameworks (COFs-AuNPs) to detect breast cancer markers (PIK3CA E545K ctDNA). The DNA walker probe is activated only in the presence of circulating tumor deoxyribonucleic acid (ctDNA), binding to a support probe to form a double strand that is then specifically cleaved by the Cas9n/sgRNA complex. This cleavage produces numerous DNA fragments for signal amplification. The COF-AuNPs as electrode materials facilitate electronic transfer and provide additional active sites for the immobilization of nucleic acid probes. This setup achieves a detection limit of 1.76 aM, demonstrating high sensitivity. Additionally, Cas9n improves the specificity of the sensor, accurately distinguishing a pair of base-mismatched sequences, and reducing the occurrence of false positives. Overall, the sensor exhibits excellent selectivity, reproducibility, and potential for early diagnosis of breast cancer. Graphical Abstract
研究领域:
生物技术
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癌症生物学
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纳米技术
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电化学
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分子诊断
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Mengying Liu,Jia Gao,Huixiao Yang,Yidi Zhang,Xinzhu Huang,Kaoqi Lian,Li Ma
Perfluorinated compounds (PFCs) are a class of widely distributed persistent organic pollutants (POPs), attracting increased attention. Consequently, there is an urgent demand for sensitive, reliable, and high-throughput methods to detect PFCs. This study used a monomer-mediated in situ growth strategy to design and synthesize Fe 3 O 4 @TFA–TAPA–COF. Accordingly, a novel method based on Fe 3 O 4 @TFA–TAPA magnetic solid-phase extraction combined with ultra-high-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) was developed to detect 22 ultra trace PFCs from water and honey samples. Several techniques used to characterize Fe 3 O 4 @TFA–TAPA–COF showed that it had a porous structure with a large surface area (102.84 m²/g). Under optimized parameters, the ranges for the limit of detection (LOD) in water and honey were 0.42–23.44 pg/L and 3.36–187.52 pg/kg, respectively. The results indicated that Fe 3 O 4 @TFA–TAPA–COF had high selectivity, high adsorption ability, and reusability. Additionally, the proposed method exhibited good accuracy, low LOD, and good reproducibility. This method was successfully applied to determine PFCs in 54 tap water and 40 honey samples in Shijiazhuang, China, and provided methodological reference and technical support for the risk monitoring of food quality and safety.
研究领域:
环境科学
·
分析化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）