Frontiers in Nutrition
·
2026
·
IF 5.5
Yao Sun,Wei Zheng,Xianling Yang,Jiayu Deng,Qipin Gao,Duoduo Xu,Yang Gao
IntroductionGrifola frondosa is an edible and medicinal mushroom rich in bioactive polysaccharides, but the relationships among molecular-weight distribution, compositional and linkage features, biological activity, and in vivo distribution remain incompletely characterized.MethodsPolysaccharides from G. frondosa were extracted, purified, and separated into three molecular-weight fractions: PP-1 (>10 kDa), PP-2 (1-10 kDa), and PP-3 (< 1 kDa). The fractions were characterized by total sugar, acidic sugar and protein contents, monosaccharide composition, apparent molecular-weight distribution, and methylation analysis. Their preliminary bioactivities were evaluated using S180, HepG2, and RAW 264.7 cell-based assays. PP-3 was subsequently labeled with fluorescein isothiocyanate followed by iodination, and iodine content was quantified using inductively coupled plasma mass spectrometry (ICP-MS) to evaluate its preliminary in vivo distribution.ResultsAmong the three fractions, PP-3 showed the highest sugar content and a relatively simple detected monosaccharide profile under the present Glc/Gal-standard-based analysis, with methylation analysis suggesting abundant terminal Glcp residues and limited internal glucose linkages. PP-3 exhibited the strongest preliminary in vitro antiproliferative activity against S180 sarcoma and HepG2 hepatocellular carcinoma cells under the present screening conditions. It also enhanced RAW 264.7 macrophage proliferation and increased nitric oxide, TNF-α, IL-1β, and IL-6 production. The ICP-MS method showed good linearity, precision, stability, reproducibility, and recovery. After oral administration in rats, iodine-labeled PP-3-derived material showed limited systemic absorption, with the highest blood absorption rate observed at 2 h, and iodine signal was mainly detected in the liver and kidney.DiscussionBecause ICP-MS detects iodine rather than the intact carbohydrate backbone, the biodistribution findings should be interpreted as preliminary tracer-based distribution data for iodine-labeled PP-3-derived material rather than a full pharmacokinetic profile of the native PP-3 fraction. Overall, this study identifies PP-3 as a low-molecular-weight carbohydrate/oligosaccharide-rich fraction with the strongest preliminary in vitro bioactivity among the tested G. frondosa fractions and provides an ICP-MS-based strategy for tracing iodine-labeled PP-3-derived material, supporting further investigation of mushroom-derived polysaccharide-derived fractions for functional-food and natural-product applications.
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高影响因子
TALANTA
·
2025
·
IF 6.1
Jiawei Geng,Lei Zhao,Qiang Wei,James C. Hower,Shifeng Dai
Coal fly ash is a potential source of critical metals, and accurate trace element analysis is essential for evaluating its resource value and environmental impact. Inductively Coupled Plasma - Mass Spectrometry (ICP-MS) is widely used due to its low detection limits and its capability of multi-element determination. In this study, a mixture of acids (5 mL HF + 2 mL HNO 3 for each 50 mg sample) was used for microwave-assisted digestion of fly ashes from eight Chinese coal-fired power plants and NIST Standard Reference Materials (SRMs) 1633b and 1633c.For low-alumina samples and SRM 1633c, ICP-MS results were consistent with those from X-ray fluorescence spectroscopy (XRF). However, for alumina-rich fly ashes, ICP-MS significantly underestimated most elements. In the worst case, the ICP-MS/XRF ratios for Sc, Ga, Rb, Sr, Y, Ba, La, and Ce in a single sample ranged from 0.37 to 0.68. XRD and EPMA analyses revealed that the formation of AlF 3 during digestion caused co-precipitation and trace element loss. To address this issue, a digestion method using ultrafine grinding (<23 μm) and lower test portion mass (<20 mg) was developed. This modification effectively suppressed AlF 3 formation by lowering the aluminum concentration in solution, resulting in improved ICP-MS/XRF ratios (>0.84) for alumina-rich samples. The method was further validated with additional high-alumina samples and SRMs 1633b and 1633c. This study proposes a simple yet effective pretreatment method to enhance the reliability of ICP-MS results for critical metal determination, which can serve as a valuable reference for elemental analysis of alumina-rich geological and environmental samples.
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PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2019
·
IF 3.206
Lanyun Fang,Yumei Zhang,Beibei Lu,Li Wang,Xunping Yao,Ting Ge
The present paper proposed an analytical method for speciation analysis of inorganic antimony in inhalable particulate matter, PM2.5, using high performance liquid chromatography coupling with inductively coupled plasma mass spectrometry (HPLC-ICP-MS). A new extraction method consisted of reducing extraction and oxidizing extraction using EDTA as the key component in the extractant has obtained better extraction yield and preserves stability of both species during the extraction process. Stability of Sb species in EDTA-saturated nitric or tartric acid solution and in aqueous solution were carefully investigated. Using the optimized conditions, the method allows for the determination of Sb(III) and Sb(V) in PM2.5 samples with limits of 0.10 and 0.06 ng m −3 , respectively. The precision, expressed as relative standard deviation (RSD), was always lower than 8.5% and 6.6% for Sb(III) and Sb(V), respectively. The spiked recovery rates of Sb species at three different concentration levels were in the range of 90.0–110.0%. The extraction yield of the sequential extraction process confirmed by analysis of a certified reference material of urban particulate matter, NIST1648a, furnished by National Institute of Standards and Technology was 60%. This method was applied to determine Sb(III) and Sb(V) in PM2.5 samples collected in urban area in Ningbo City, China. The results showed that both Sb(V) and Sb(III) were found in most of the samples analyzed, Sb(V) being the main one, which represents 69–87% of the total Sb extracted in the considered samples, whereas Sb(III) represents the remaining 13–31%.
研究领域:
环境科学
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分析化学
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高影响因子
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
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2017
·Vol. 32
·期 6
·1186-1195
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IF 3.379
Shan Li,Beibei Chen,Man He,Bin Hu
Protein phosphorylation analysis is important for understanding cell regulations. Mass spectrometry (MS) is a powerful technique for peptide phosphorylation analysis. However, quantification of low abundance phosphoproteins in a complex mixture of proteins is still a challenge. Here we report the development of hollow fiber (HF) supported TiO2 monolithic microextraction combined with capillary high performance liquid chromatography (capHPLC)-inductively coupled plasma-collision reaction cell (ICP-CRC)-MS for the absolute quantification of phosphopeptides. The membrane pores (∼200 nm) of the HF effectively carry more TiO2 on its surface to enhance the adherence of the TiO2 monolith with the HF. By using β-casein as a model phosphorylated protein, we optimized the HF-supported TiO2 monolithic microextraction towards phosphopeptides. Under the optimal conditions, a 100-fold enrichment factor was obtained and the monoliths can be reused 40 times. By monitoring 31P16O with O2 as a reaction gas in the CRC, polyatomic mass interference at m/z 31 is avoided and a low detection limit (2.9 nM) of phosphorus is achieved. The method of HF-supported TiO2 monolithic microextraction-capHPLC-ICP-MS provided the detection limit for phosphopeptides at the nM level. The proposed method was applied to the quantification of phosphopeptides in milk and milk powder samples.
研究领域:
蛋白质组学
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分析化学
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生物化学
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高影响因子
TALANTA
·
2022
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IF 6.556
Wenxin Cui,Zhaoqing Cai,Qing Li,Haiyun Qu,Jiaqian Zheng,Dengguang Yu,Junfeng Chen,Zheng Wang
In this study, a laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) method for in-situ determination of yttrium and trace elements in yttrium-doped barium fluoride (BaF 2 : Y) crystals was proposed. A facile, micro-damage procedure for quantifying the segregation coefficient of doping elements was investigated, and it was found that the actual yttrium doping concentration increases from the seed end to the tail end in BaF 2 : Y crystals. In micro-area analysis, this method has higher mass sensitivity which was applied to quantify the impurity content and distribution during the growth of BaF 2 : Y crystals. Regression coefficient of calibration curve for each element ranged from 0.9918 to 0.9995. Detection limits (DLs) were 0.05, 0.03, 0.01 and 0.01 μg g −1 for Mg, Zn, Sr and Pb, respectively. The accuracy of the proposed method was verified by inductively coupled plasma mass spectrometry/atomic emission spectroscopy (ICP-MS/AES) with wet-chemical pretreatment. The objective of the presented work was to provide a less damaging and more novelty approach for crystal sample analysis.
研究领域:
材料科学
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分析化学
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高影响因子
ANALYTICA CHIMICA ACTA
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2026
·
IF 6.1
Xin Li,Qiao-Hui Zhong,Jia-Kai Guo,Mei-Ju Li,Gao-Bin Chu,Jie Li
N235 extraction resin enables efficient Pb separation from geological reference materials. • Matrix are removed with dilute HBr and water, followed by Pb separation using low-concentration HBr. • The purified Pb is ready for MC-ICP-MS analysis without any evaporation step. • The method reliability is validated using various reference materials. • Pb isotope ratios of JA-1a are reported for the first time. Lead isotopic variations provide crucial insights into geochemical reservoirs, environmental sources, and geological ages. To obtain precise Pb isotope data by MC-ICP-MS, we develop a simple and efficient separation procedure using trialkyl amine (N235) resin. Batch experiments show that N235 strongly adsorbs Pb in 0.4 mol L −1 HBr, weakly in HCl, and not at all in HNO 3 media. Specifically, the optimized column procedure achieved high Pb recovery (94.5%) and eluted Pb in 0.5 mol L −1 HNO 3 , allowing direct measurement without evaporation, thereby reducing procedural blanks. The method was validated with ten reference materials (GSR-1, GSR-2, GSR-3, GSR-7, GSP-2, BHVO-2, JA-2, JG-2, JB-2, and JA-1a). Measured 206 Pb/ 204 Pb, 207 Pb/ 204 Pb and 208 Pb/ 204 Pb ratios agreed with published values within uncertainty, with external reproducibility better than 0.05% (RSD). Owing to its simplicity, high recovery, low blank, and low cost, this method offers a practical pretreatment strategy for high-precision Pb isotope analysis in diverse geological samples. A very simple, low-blank, and effective Pb purification method with a self-made trialkyl amine extraction resin is developed to separate Pb from geological reference materials for high precision determination of Pb isotopic compositions by MC-ICP-MS. Download: Download high-res image (254KB) Download: Download full-size image
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高影响因子
SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY
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2025
·
IF 3.8
Yufeng Li,Hanxu Ji,Jingjing Xu,Xiuming Mei,Xiaoxiao Wu,Yongli Ye,Miao Yang,Diyao Jiang,Yuwei Qiang,Lan Jiang,Jungui Zhou,Chi Zhang,Xiulan Sun
A novel method for detecting Tilletia foetida teliospores in wheat was developed by integrating inductively coupled plasma mass spectrometry (ICP-MS) with an elemental labeling strategy. On the basis of an immunological method, capture magnetic beads coupled with a polyclonal antibody probe (MBs-pAb) were constructed to specifically enrich and label T. foetida teliospores. A gold nanoparticle-labeled monoclonal antibody was then added to bind to MBs-pAb. After dissociation of the resulting complexes using citric acid, the 197 Au signal was detected by ICP-MS, allowing for the quantitative detection of T. foetida spores. Under optimal conditions, the method had a limit of detection of 50 teliospores/mL and a linear range of 200–50,000 teliospores/mL. This new approach, which enables the rapid and highly sensitive detection of teliospores within 2.5 h, was successfully applied to wheat samples. The method shows significant potential for analyzing and monitoring common bunt of wheat, offering a robust tool for agricultural diagnostics and disease management.
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高影响因子
GEOSTANDARDS AND GEOANALYTICAL RESEARCH
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2024
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IF 2.7
Xiuhong Liao,Tao Chen,Tao Luo,Yue Cai,Wen Zhang,Zuowei Yin,Zhaochu Hu
Tourmaline serves as a vital recorder of geological processes. However, suitable tourmaline reference materials (RMs) for elemental and stable isotopic composition in situ measurements are still limited. In this study, three tourmaline megacrysts (MD-B66, IM-B232 and BR-DG68) were characterised as potential RMs for B isotope measurements by LA-MC-ICP-MS and mass fraction determinations by LA-ICP-MS. Over 251 measurements with LA-MC-ICP-MS on ten randomly selected fragments from MD-B66 consistently yielded B isotope ratios of -7.74 ± 0.25‰ (2 s ), establishing MD-B66 as a suitably homogeneous primary reference material for high-precision, in situ microbeam B isotope measurements. Notably, the (long-term) intermediate precision of 0.25‰ (2 s ) for in situ B isotope measurements obtained using this reference material is comparable to that reported from solution MC-ICP-MS methods in the literature. Two other tourmaline megacrysts, with intermediate precision ranging from ± 0.48‰ to ± 0.61‰ (2 s ) for δ 11 B measurement, can be employed as secondary RMs for quality control. The mean δ 11 B values determined by solution MC-ICP-MS for MD-B66 (-7.71 ± 0.32‰, n = 12), IM-B232 (-13.17 ± 0.62‰, n = 8) and BR-DG68 (-13.85 ± 0.32‰, n = 12), with expanded uncertainties at the 95% confidence level, are consistent with those determined by LA-MC-ICP-MS. Among these three new RMs, BR-DG68 displays relatively homogeneous major and trace element mass fractions. Characterisation using both in situ and wet chemical techniques demonstrated the suitability of BR-DG68 as the first tourmaline reference material for elemental measurement by LA-ICP-MS, which would permit matrix-matched and therefore more accurate elemental measurement in tourmalines. Unlike electron probe microanalysis with B and Li contents calculated based on stoichiometric assumptions, direct and accurate measurements of the two low atomic number elements, along with other major and trace elements can be achieved by LA-ICP-MS with the aid of the newly developed tourmaline reference material BR-DG68. Overall, current and future studies in geochemistry may benefit from these newly proposed tourmaline RMs, which should lead to significantly improved precision and accuracy for in situ B isotope and elemental measurement.
研究领域:
地质学
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地球化学
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高影响因子
TALANTA
·
2026
·
IF 6.7
Jisheng Chen,Youyi Ni,Wenting Bu,Yue Luo,Bingjun Chen,Xuemei Liu,Chuting Yang,Junwen Wu,Hai Wang
Highly efficient and accurate analysis of ultra-trace anthropogenic 236 U in the presence of natural uranium isotopes is essential for nuclear forensics and environmental monitoring. In this study, we developed an integrated analytical method for the determination of 234 U, 235 U, 236 U, and 238 U by combining highly automated sample preparation with highly sensitive ICP-MS/MS detection. Automated total dissolution was employed to ensure complete extraction of both endogenous and exogenous uranium from solid matrices, while reproducible chemical separation was achieved using an automated platform equipped with regenerable UTEVA resin. This optimized procedure yielded exceptionally low operational blanks for 236 U at femtogram level. Moreover, the final eluent volume was minimized to 1.5 mL, allowing direct introduction into the ICP-MS/MS. By utilizing a membrane desolvation sample introduction system and a novel mass-shift mode (targeting UO 2 + species), the ICP-MS/MS sensitivity exceeded 4.1×10 6 cps/ppb, while the 235 UH + interference formation rate for 236 U was suppressed to 7.1×10 -10 . Consequently, the achieved detection limits for 236 U and the 236 U/ 238 U atom ratio were as low as 1.85 fg/g and 5×10 -12 , respectively. This method was successfully applied to determine the uranium isotopic composition in sediments from the adjacent sea area of the Daya Bay Nuclear Power Plant. These results demonstrate that the proposed method provides a robust and high-throughput solution for accurately quantifying of ultra-trace 236 U in environmental samples, such as those impacted by the global fallout.
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高影响因子
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
·
2015
·Vol. 31
·期 3
·679-685
·
IF 3.466
WeiJuan Yang,Zhiming Xi,Xiaoxue Zeng,Ling Fang,Wenjing Jiang,Yongning Wu,LiangJun Xu,FengFu Fu
Early diagnostic methods are the most effective way to increase the survival rate of cancer patients. In this study, we present a novel method for sensitively and specifically counting cancer cells using a magnetic bead (MB)-based AuNP–aptamer labelling technique and inductively coupled plasma mass spectrometry (ICP-MS) detection. A MB-based AuNP–aptamer labelling technique can specifically recognize cancer cells and tag AuNPs to the cancer cells, which provides a platform for indirectly counting cancer cells via the detection of Au with ICP-MS. The method reported in this study combines the merits of a MB-based AuNP–aptamer labelling technique and ICP-MS detection, resulting in better specificity and stability, high sensitivity, short analysis time, and robust resistance to a complicated matrix. Especially, the employment of ICP-MS detection and the presence of MBs that facilitate the magnetic separation of target cells, not only greatly shorten the analysis time but also greatly enhance the resistance of our method to a complicated matrix. The proposed method can be used to count as few as 100 human hepatocellular carcinoma SMMC-7721 cells in 1 mL of serum with a recovery of 93–104% and a RSD < 4% (n = 5) within 3 hours. The success of this study provides a promising rapid approach for the early detection of human hepatocellular carcinoma cells in clinical diagnosis.
研究领域:
分析化学
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癌症生物学
·
医学诊断
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高影响因子
ANALYTICAL LETTERS
·
2024
·
IF 1.6
Chaoying Zhang,Qin Zhang,Yingchun Zhao,Dianquan Dong,Longjiang Huang
Monitoring TiO2 nanoparticles (NPs) that are released from functional textiles is significant for assessing the potential risk to consumers. However, determining the size distribution of TiO2 NPs is still a challenge due to agglomeration. The present study employed single particle–inductively coupled plasma–mass spectrometry (SP-ICP-MS) to quantify TiO2 NPs released from functional textiles. Through screening dispersants and validating the standard samples, an accurate method for determining the size and number of TiO2 NPs was developed. The validated procedure was successfully used to determine the release of TiO2 NPs from yarn in sweat and water media throughout the day. The result revealed that the yarn decorated with TiO2 NPs released approximately the same particle size distribution from 60 to 260 nm in both media. The released particle concentration was 0.094 µg/mL with a mean size of 136 nm in water and a particle concentration of 0.120 µg/mL with a mean size of 133 nm in sweat. Additionally, based upon transmission electron microscopy, anatase TiO2 NPs were identified. It is expected that this work is significant in the evaluation of the toxicological effects of multifunctional textiles modified by TiO2 NPs on human health.
研究领域:
环境科学
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毒理学
·
纳米材料科学
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高影响因子
NanoImpact
·
2016
·
IF 0
Xiao-Xia Zhou,Jing-Fu Liu,Fang-Lan Geng
Analysis of metal oxide nanoparticles (MO NPs) and their released metal ions (M n+ ) is essential for understanding their environmental effects and biological toxicity. For the first time, size exclusion chromatography (SEC) was coupled with inductively coupled plasma mass spectrometry (ICP-MS) for speciation analysis of MO NPs and their corresponding M n+ . By using a 1000 Å poresize silica column, and an acetate buffer (pH 7.0, 10 mM) containing 10 mM sodium dodecyl sulfate (SDS) as a mobile phase at a flow rate of 0.5 mL/min, M n+ and MO NPs with sizes smaller than the column poresize were baseline separated within 10 min, whereas MO NPs with sizes larger than the exclusion limit were filtered off by the column. More importantly, this mobile phase is able to avoid the dissolution of MO NPs during the SEC separation. The high recoveries (> 97%) of M n+ from the SEC column ensured their accurate quantification directly with the online coupled SEC-ICP-MS, while the quantification of MO NPs by subtracting the M n+ content from the total metal content, which was determined with ICP-MS after digestion without SEC separation, efficiently eliminating the influence of retention of large MO NPs in the column. MO NPs with different sizes and compositions including NiO, CoO, ZnO, CuO and CeO 2 NPs were analyzed with this proposed method, with low detection limits (0.016–0.390 μg/L for both M n+ and MO NPs) and relative standard deviations (< 0.4% for retention time and < 2.2% for peak area at 50 μg/L M n+ ). The proposed method was verified by analyzing Ni 2+ , Ce 4+ , NiO and CeO 2 NPs in various environmental waters, with spiked recoveries in the range of 80.8–105.1%. Our method has great potential for speciation analysis of MO NPs and their corresponding M n+ in the environmental waters.
研究领域:
分析化学
·
环境科学
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