BIOMACROMOLECULES
·
2019
·
IF 5.667
Yanqian Wu,Yi Xiao,Yushu Huang,Yanyun Xu,Donglei You,Wei Lu,Jiahui Yu
A biodegradable brush-type copolymer PHF-g-(PCL-PEG) based on a cleavable polyacetal backbone and biodegradable side chain modified with polyethylene glycol (PEG) was synthesized in this paper. This particular structure was directional to facilitate the formation of spherical or rod-shaped micelles. Flow cytometry showed that rod-shaped micelles displayed enhanced cellular uptake compared to spherical micelles. Rod-shaped micelles were selected to investigate their drug delivery abilities in detail. In vitro experiments verified the pH-triggered drug release of DOX-loaded micelles, and the release rate of doxorubicin (DOX) was 77% at pH 5.0 and 26% at pH 7.4. In drug-release kinetic analysis, a double-exponential model achieved the best fit. The copolymer appeared to be almost nontoxic, while the DOX-loaded micelles showed equivalent cytotoxicity compared to DOX at high concentration. The endocytosis of DOX-loaded micelles was two times that of DOX. Our findings suggest that the pH-sensitive brush type copolymer could be a possible carrier in drug delivery.
研究领域:
生物材料学
·
药物递送系统
·
高分子化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2011
·
IF 2.971
Jianian Chen,Huijuan He,Shaoshun Li,Qi Shen
The aim of this study is to develop a simple and applicable HPLC method for the detection of vincristine in rat plasma after administration of poly(lactic- co -glycolic acid)–poly(ethylene glycol) (PLGA–PEG) nanoparticles loaded with vincristine sulfate (VCR). Vincristine was extracted from rat plasma and vinblastine sulfate was chosen as the internal standard (IS). Chromatographic separation of VCR and IS was achieved by a Dikma Dimonsil C 18 column (200 mm × 4.6 mm) with the mobile phase consisting of 0.02 M sodium dihydrogen phosphate–methanol (36:64, v/v, pH = 4.7) at a flow rate of 1.0 mL/min. The ultraviolet detection wavelength was set at 276 nm. The calibration curve was linear over a concentration range of 0.05–5.0 μg/mL. The intra-day and inter-day accuracy for three quality controls (QC) samples was 93.48–107.74% and 92.61–96.58%, respectively; the precision was less than 9%. The average method recoveries for vincristine from spiked plasma at all QC levels were over 83%; and extraction recoveries were between 66 and 70%. Vincristine was stable in rat plasma for one month at −80 °C, for 8 h at room temperature , as well as during three freeze–thaw cycles. This HPLC method was applied successfully to the pharmacokinetic study of vincristine in rats after a single intravenous injection of VCR in physiological saline (F-VCR) solution, VCR-loaded PLGA–mPEG nanoparticles with (NP1) and PLGA–PEG–folate nanoparticles (NP2) suspension, respectively. There were significant differences in main pharmacokinetic parameters between F-VCR and the nanoparticles. Both kinds of VCR-loaded nanoparticles displayed improved pharmacokinetic profiles.
研究领域:
药理学
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生物医学工程
·
分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF TOXICOLOGICAL SCIENCES
·
2019
·
IF 1.732
Yuan Chen,Xiaoxiao Fei,Chenqiao Ye,Qinqing Qian,Zhiqiu Ye,Siqi Xie,Jiamin Chen,Mingliao Zhu,Na Ran,Mingsheng Hou,Lin Xu,Zhangsen Yu
In the past few decades, upconversion nanoparticles (abbreviated as UCNPs) have been more widely applied in the biomedical fields, such as in vitro and in vivo upconversion fluorescent bioimaging, photodynamic therapy, biological macromolecular detection, imaging mediated drug delivery and so on. But meanwhile, there is still not much research on the acute toxicity of upconversion nanoparticles in vivo, such as acute hepatotoxicity. In this work, we studied the in vivo biodistribution and acute hepatotoxicity of multimodal targeted contrast agent NaLuF4:Gd,Yb,Er-PEG/PEI-FA nanoprobe, which were synthesized by the solvothermal method and modified with Polyethylene glycol (PEG), Polyetherimide (PEI), folic acid (FA) on the surface. The acute hepatotoxicity in mice was systematically assessed after tail vein injection of different concentration of UCNPs. The results showed that NaLuF4:Gd,Yb,Er-PEG/PEI-FA nanoparticles with an average diameter of 44.5 ± 10.4 nm, and three typical upconversion fluorescence emission bands at 520 nm, 540 nm and 660 nm under the excitation of 980 nm laser. In vivo distribution experiments results demonstrated that approximately 87% of UCNPs injected through the tail vein accumulate in the liver. In the acute hepatotoxicity test, the intravenously injection dose of UCNPs was 10, 40, 70 and 100 mg/kg, respectively. The body weight, blood routine, serum biochemistry, histomorphology and liver oxidative stress were detected and observed no significant acute hepatotoxicity damage under the injection dose of 100 mg/kg. In conclusion, NaLuF4:Gd,Yb,Er-PEG/PEI-FA nanoprobes are safe and reliable, and have potential applications in the field of tumor targeted multimodal imaging.
研究领域:
生物医学工程
·
毒理学
·
纳米技术
·
分子影像
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2017
·
IF 3.671
Yurui Xu,Sajid Asghar,Liu Yang,Zhipeng Chen,Hongying Li,Wenwen Shi,Yibo Li,Qinqing Shi,Qineng Ping,Yanyu Xiao
Nanoparticles based on chitosan hydrochloride (CSH)-hyaluronic acid (HA)-PEG were prepared for delivering curcumin (CUR) (CUR-PNPs) to brain tumor. CUR-PNPs of 245.9 nm and spherical morphology were obtained at optimized CSH/HA/PEG20000/CUR ratios with negative charge of about −27.2 mV and EE of approximately 93.3%. Cytotoxicity studies showed that CUR-PNPs improved drug’s anticancer activity in rat glioma cells (C6). The cellular uptake mechanism study showed active targeting of CUR-PNPs into C6 cells by HA mediated endocytosis . Clathrin-coated pit mediated endocytosis, clathrin-mediated endocytosis and macropincytosis were also identified as the entry pathways of PNPs into C6 cells. Pharmacokinetics of preparations in rats after i.v. administration further proved the superiority of CUR-PNPs (3.98 times greater than the area under the curve of CUR solution). In conclusion, CUR-PNPs might be a promising carrier for the therapy of brain tumors.
研究领域:
药学
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肿瘤学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
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2019
·
IF 7.015
Haicheng Jiang,Qi Zhao,Panpan Wang,Jun Ma,Xuedong Zhai
Fabricating high-flux, low-fouling gravity-driven membranes (GDM) has proved to be an extremely challenging work. In this study, a novel tailored amphiphilic multi-arms polymer poly(propylene glycol)-silane-poly(ethylene glycol) (PPG-Si-PEG) was synthesized and blended into Polyvinylidene Fluoride (PVDF) matrix to fabricate GDM via nonsolvent induced phase separation method. The incorporation of multi-arms polymers affected phase separation and surface segregation behaviors, resulting in the formation of more porous structure, which were demonstrated by scanning electron microscopy (SEM). Moreover, the hydrophilic PEG arms in polymers were enriched onto membrane surface through surface segregation and utilized to improve the hydrophilicity of membranes, which were confirmed by Fourier transform infrared (FTIR), X-ray photoelectron spectroscopy (XPS) and contact angle measurement. The separation and antifouling properties of the membranes were evaluated by using gravity as the driving force, employing bovine serum albumin (BSA) as the model foulant. The water flux of membranes increased from 4.6 L m −2 h −1 for the control membrane to 12.1 L m −2 h −1 for PVDF/PPG-Si-PEG∼5% membrane. The antifouling properties of modified membranes were effectively improved in comparison with that of control membrane. The flux recovery ratio, especially for PVDF/PPG-Si-PEG∼5% membrane, was up to 99.4%. This study provided a new method for antifouling modification of GDM.
研究领域:
材料科学
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环境工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS
·
2016
·Vol. 54
·期 16
·1612-1623
·
IF 3.318
Yunjiao Gu,Shuxue Zhou,Huali Luo,Limin Wu,Wei Gao,Jinlong Yang
Controllable phase segregation adjustment for immiscible polymer blends has always been tough, which hinders the development of amphiphilic antifouling coatings from more accessible blends. Herein, methacrylated poly(dimethylsiloxane) (PDMS-MA) was synthesized and mixed with poly(ethylene glycol)methylether methacrylate (PEG-MA). It was interestingly discovered that these PDMS-MA/PEG-MA blends displayed upper critical solution temperatures (UCST) due to thermo-induced conformational change of PEG-MA and the UCST changed with PDMS-MA/PEG-MA mass ratios. Micro-/nano-phase segregation, nanophase segregation, or homogenous morphology were therefore achieved. These PDMS-MA/PEG-MA blends with different mass ratios were UV-cured under varying temperatures to fabricate coatings. Their surface morphology and wettability are readily adjusted by phase segregation. For the first time, highly hydrophilic surface was achieved for coatings with microphase segregation because of the exposure of PEG-rich domains, which exhibited an enhanced protein resistance against bovine serum albumin (BSA). Anti-bacterial performance ( Shewanella loihica ) was also observed for these PDMS/PEG coatings. © 2016 Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2016 , 54 , 1612–1623
研究领域:
材料科学
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生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
COLLOID AND POLYMER SCIENCE
·
2016
·Vol. 295
·期 1
·259-266
·
IF 1.89
Shi Yanan,Zhu Haibo,Ren Yan,Li Keke,Tian Baocheng,Han Jingtian,Feng Dongxiao
Poly(ethylene glycol)-poly(D,L-lactide) (PEG-PLA), a type of copolymer widely used for drug delivery, was synthesized in the present work based on previous studies, which is used for bovine serum albumin (BSA)-loaded micelle formulation. The micelles exhibited less than 100 nm in size with narrow polydispersity indices (PDI) of smaller than 0.3, and showed an encapsulation efficiency of 69%. BSA was encapsulated in the PEG-PLA micelle successfully exhibiting intact structure indicated by native polyacrylamide gel electrophoresis (Native-PAGE), fluorescence spectroscopy and fourier transform infrared spectroscopy (FTIR) analysis methods. It investigated the effects of freeze-drying, varying amount of cryoprotectants (sucrose, mannitol and lactose), and ultrasonication treatment on the size, and size distribution. The micelles were agglomerated in the freeze-drying process demonstrated by transmission electron microscope (TEM) and dynamic light scattering (DLS) analysis. Five percent of mannitol and ultrasonication for 90 s were found producing a significant reduction in agglomeration as well as a sharp decrease in size. The results demonstrated suitable cryoprotectant, and ultrasonication are beneficial to control the size and distribution of protein-loaded PEG-PLA micelle.
研究领域:
药剂学
·
药物递送
·
聚合物科学
·
蛋白质工程
✓ 已验证
产品关联
DOI
高影响因子
Nanomaterials
·
2023
·Vol. 13
·期 2
·268
·
IF 5.3
La Shi,Xiaolong Fu,Yang Li,Shuxin Wu,Saiqin Meng,Jiangning Wang
The mechanical properties of HTPE/PEG interpenetrating polymer network (IPN) binders were systemically studied with molecular dynamics (MDs) simulations and experiments. In this study, an algorithm was used to construct the crosslinking interpenetrating polymer network models and then the mechanical behaviors of Hydroxyl-terminated polyethylene glycol-tetrahydrofuran co-polyether/poly ethylene glycol (HTPE/PEG) IPN models were analyzed at a molecular scale. Firstly, glass transition temperatures (Tg), mean square displacement (MSD) and mechanical properties of IPN crosslinked model simulations showed that better thermomechanical parameters appeared at low temperatures, which were in good agreement with the experimental methods, including dynamic mechanical analysis and uniaxial tensile. Then bond-length distribution was performed to verify the crosslinked structures between prepolymers and curing agents. FTIR-ATR spectra analysis of four IPN binder specimens also gave a convictive result to the special interpenetrating polymer network of polyether polyurethane binders. Cohesive energy density and friction-free volume explained how the micro-structures of IPN crosslinked models and the force of inter-molecule chains affected the mechanical behaviors of the HTPE/PEG polyurethane matrix. Lastly, the morphology of IPN binder specimen tensile fracture indicated the mechanism at different temperatures. These studies were helpful in understanding the mechanical properties of HTPE/PEG interpenetrating polymer network binders and provide molecular insight into mechanisms of mechanical behaviors, which would guide the property improvement of HTPE propellant.Keywords:HTPE/PEG;IPN;mechanical properties;molecular dynamic simulation;crosslinking structures
研究领域:
高分子科学
·
材料科学
·
机械工程
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产品关联
DOI
PubMed
高影响因子
Artificial Cells Nanomedicine and Biotechnology
·
2019
·
IF 4.462
Dinglingge Cao,Xingxian Zhang,MD. Akabar,Yuan Luo,Hao Wu,Xue Ke,Tianyuan Ci
The aim of this research is to utilize a hybrid system of liposomal doxorubicin (DOX-Lip) loaded thermogel (DOX-Lip-Gel) to realize the steady sustained delivery of doxorubicin (DOX), a small hydrophilic drug, for the treatment of breast cancer locally. Herein, liposomal doxorubicin was prepared via the traditional film dispersion method with the particle size of 75 nm and drug entrapment efficiency of 86%. And, the triblock copolymer of poly (D, L-lactide-co-glycolide)-b-poly (ethylene glycol)-b-poly (D, L-lactide -co-glycolide) (PLGA-PEG-PLGA) was synthesized via ring-opening polymerization to prepare the thermosensitive hydrogel through dissolving the polymers in DOX-Lip solution. The liposome loaded hydrogel was in a sol state at room temperature and converted into the gel state at body temperature and would degrade gradually during the time in vivo. The drug release of DOX out of DOX-Lip-Gel could be in a steady sustained manner up to 11 days without significant burst release as compared to that of DOX-loaded hydrogel (DOX-Gel). An orthotopic breast cancer model was adopted to evaluate the in vivo antitumor efficacy. And, the results revealed DOX-Lip-Gel had better antitumor efficiency as well as lower side effects.
研究领域:
药剂学
·
肿瘤学
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Science China-Materials
·
2024
·
IF 6.8
Ma Yanyan,Li Jifeng,Ding Tao,Feng Qichun,Du Zhaofang
Water loss rate is crucial in evaluating the efficiency of atmospheric water harvesting (AWH) materials. However, most moisture-absorbing salts and gels have fixed heat transfer rates, limiting the development of high-performance AWH materials. Herein, an anisotropic PEG/CS/MF nanocomposite (APCM) with adjustable thermal transfer efficiency is presented. APCM was synthesized using polyethylene glycol (PEG), melamine foam (MF), and chitosan (CS) solution through a freeze orientation method. The resulting material exhibits a stable oriented laminated structure formed by hydrogen bonding between PEG, CS, and MF. This unique structure imparts excellent mechanical properties. APCM’s large lamellar gaps and pore diameters enable rapid absorption of atmospheric water molecules at low temperatures without leakage (61.79 kg m −3 ). The compressible nature of APCM allows for efficient heat transfer at high temperatures, and the release of 80% of absorbed water within 15 min. In a proof-of-concept demonstration using a custom-built AWH device, each cubic meter of APCM achieved three AWH cycles within 24 h, producing over 185 kg of water. Therefore, this innovative design offers a promising solution for enhancing the efficiency of AWH, potentially addressing water scarcity issues in various regions.
研究领域:
材料科学
·
环境科学
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Zheyu Li,Yue Xiong,Shuangli Li,Jiang Zhu,Rui Hu,Ying Li,Yunhuang Yang,Maili Liu
Residual dipolar couplings (RDCs) are widely used in NMR for the investigation of protein structure and dynamics. However, commonly used RDC media ( e.g. , C 12 E 5 /Hexanol/H 2 O or PEG system, lipid bilayer or bicelle system) are unstable viscous liquids, making it challenging to obtain stable and homogeneous NMR samples by laborious manual operation. In this paper, we report a unique fishhook-shaped micromixer (FS-mixer) that enables fast and thorough mixing of high-viscosity PEG and bicelle media for RDC analysis. Numerical simulations and experimental characterizations proved that the micromixer could achieve a complete mixing of viscous liquids in 382.4 μs, improved significantly over current mixers. In compared to manually prepared RDC samples, the on-chip prepared samples have a better uniformity and alignment efficiency, and show more stable peak split in the 2 H spectrum. As a proof of concept, we prepared a RDC sample for INSM1 ZF1-2 protein by using this FS-mixer. The IPAP-HSQC spectra demonstrated that this strategy can efficiently prevent the generation of low resolution and distorted spectra, which could facilitate further refinement of the NMR structure. This micromixer-assisted sampling method greatly simplifies the procedure, increases the media uniformity and stability, and shortens the time in RDC analysis. We believe it could serve as a useful tool in RDC-based NMR analysis.
研究领域:
生物物理学
·
分析化学
·
结构生物学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Nian Jiang,Ling Qian,Qiang Peng,Shuqi Zhang,Wanqing Yue
Hydrogen peroxide (H 2 O 2 ) is produced in living organisms and is involved in a variety of redox-regulated processes. Therefore, the detection of H 2 O 2 is important for tracing the molecular mechanisms of some biological events. Here, we demonstrated for the first time the peroxidase activity of PtS 2 -PEG NSs under the physiological conditions. PtS 2 NSs were synthesized by mechanical exfoliation followed by functionalization with polyethylene glycol amines (PEG-NH 2 ) to improve their biocompatibility and physiological stability. Fluorescence was generated by catalyzing the oxidation of o-phenylenediamine (OPD) by H 2 O 2 in the presence of the PtS 2 NSs. The proposed sensor had a limit of detection (LOD) of 248 nM and a detection range of 0.5–50 μM in the solution state, which was better than or comparable to previous reports in the literature. The developed sensor was further applied for the detection of H 2 O 2 released from cells as well as for imaging studies. The results show that the sensor is promising for future applications in clinical analysis and pathophysiology.
研究领域:
生物化学
·
医学诊断
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）