LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Shuxian Zhang,Xiaofei Wang,Xiaoxuan Fan,Keshuai Liu,Gidion Wilson,Xueqin Ma,Guoning Chen
Lactose can cause health problems in patients with lactose intolerance. β-Galactosidase is commonly used to catalyze the hydrolysis of lactose, thereby producing low-lactose or lactose-free products. However, most existing methods employ free enzymes, which significantly increases the economic cost. In this study, the polyurethane sponge (PUS) was selected as the carrier, and the cross-linked enzyme aggregates (CLEAs) were loaded onto the carrier after the sponge was modified. The reaction products were characterized by scanning electron microscopy, X-ray photoelectron spectroscopy, Fourier infrared transform spectroscopy, and simultaneous thermal analysis, respectively. The loading amount of β-galactosidase under the optimal conditions was 63 U/g support . The prepared PUS@β-galactosidase-CLEAs was successfully used for the hydrolysis of lactose in milk and could hydrolyze 228–304 μg of lactose in 30 min with good stability (RSD = 4.4%) and reusability. The method proposed in this study effectively combined the advantages of sponge and cross-linking enzyme aggregates, successfully achieving the decomposition of lactose in milk and rapid separation of the product. It is worth mentioning that this study provided a more effective and convenient industrial option for the production of lactose-free milk.
研究领域:
食品科学
·
生物工程
✓ 已验证
产品关联
DOI
高影响因子
Joule
·
2026
·
IF 37.1
Haoxuan Liu,Yating Shi,Wei Ran,Hua Zhong,Fei Zhang
Considering temperature variations is crucial for the practical applications of perovskite solar cells (PSCs). However, to date, no studies have reported mitigating potential lead-leakage issues in PSCs after thermal cycles (ISOS-T). In this study, we design and synthesize a stable lead adsorption film, PGP, which maintains its high flexibility and optical transparency over numerous days of thermal cycling. Under simulated harsh environmental conditions, the lead sequestration efficiency (SQE) of the film remains above 98% for both flexible and rigid PSCs and modules, without negatively affecting the performance and stability. After thermal cycling tests according to ISOS-T protocols, this film still maintains an SQE above 98%. Additionally, the film also demonstrates a 99% SQE in perovskite-silicon tandem solar cells. This is the first report to achieve a high SQE in rigid and flexible devices after thermal cycling, representing a significant step forward toward the commercialization and practical applications of PSCs.
✓ 已验证
DOI
高影响因子
Journal of Environmental Sciences
·
2025
·
IF 6.3
Junhui Li,Hanjie Chen,Hongling Bu,Yanfu Wei,Meiqing Chen,Wenpo Xu,Xiangying Hao,Peng Yuan
The interaction between divalent iron (Fe(II)) and humic substances (HS) is crucial for geochemical cycles in terrestrial and aquatic environments, influencing soil phosphorus (P) cycling and aquatic eutrophication. However, the impact of Fe(II)-HS interactions on P sequestration remains unclear. This research employed controlled lab experiments and advanced characterization techniques, such as X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), and Scanning Electron Microscopy (SEM), to study the mechanisms of P fixation by Fe(II)-HS complexes under neutral pH and anaerobic conditions. Results indicate that Fe(II) availability is crucial for P fixation, more so than HS content. Elevated Fe/P ratios boost P removal by encouraging vivianite formation, even in the presence of HS, reducing dissolved P to below 0.3 mg/L at Fe/P of 2.2. HS primarily hinders P adsorption through competitive ligand exchange. Solution pH also impacts P fixation, with higher pH levels (≥7) enhancing P immobilization. While HS binds Fe(II) and prevents hydrolysis, Fe(II) hydrolysis products like Fe(OH) 2 /Fe(OH) 3 − can co-precipitate and remove P. XRD analysis indicated vivianite crystals form at pH 7, but their diffraction peak intensity decreases under acidic or alkaline conditions, with formation controlled by Fe species and HS. The potential pathways of P in the Fe(II)-HS system include P combining with Fe(II) to form vivianite, P combining with HS to form HS-P composites, and P combining with Fe(II)-OM species to form carbon-containing vivianite. These insights improve understanding of the Fe-C-P biogeochemical cycle and P mobility in natural environments.
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产品关联
DOI
高影响因子
POLYMER ENGINEERING AND SCIENCE
·
2014
·Vol. 55
·期 5
·1039-1045
·
IF 1.441
Huimin Qi,Hongdong Duan,Xingjian Wang,Xia Meng,Xiaocong Yin,Lina Ma
In this work, a series of porous terpolymers with degrees of crosslinking at 30–65% were synthesized by suspension polymerization. Fe 3 O 4 nanoparticles were used to modify the porous terpolymer with degree of crosslinking at 30%. The resulting terpolymers with mean pore size of 10–30 nm were used as supports for immobilization of cellulases through covalent bondings. The Fourier transform infrared spectroscopy and thermal gravity analysis measurements demonstrated that cellulases were immobilized onto the porous terpolymers. The effects of pH and temperature on enzyme activity were investigated in acetate buffer medium by hydrolysis of sodium carboxymethyl celluloses. The reusability of immobilized cellulases was also investigated. The result showed that immobilized cellulases exhibited higher residual activities and enzyme activities than free cellulases in wide ranges of pH and temperature. We also found that the reusability of cellulases immobilized on terpolymers with magnetism was better than that of cellulases immobilized on terpolymers without magnetism. POLYM. ENG. SCI., 55:1039–1045, 2015. © 2014 Society of Plastics Engineers
研究领域:
高分子科学
·
酶工程
·
纳米材料
·
生物催化
·
应用化学
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Xinru Yang,Yuxin Zhang,Chunhui Zhang,Fuyu Li,Cuiping Wang,Zhiyuan Zhang,Kai Yu,Hongwen Sun,Baoshan Xing
Waste-derived zeolite efficiently removes Cd across various pH conditions. • pH conditions drive the shift in Cd removal mechanisms on zeolite. • Hydroxyl groups in soil dissolved organic matter enhance Cd fixation on zeolite. • Sustainable strategy integrates waste reuse with remediation. Soil dissolved organic matter (DOM), which is ubiquitously released during remediation of heavy metal-contaminated soil-water systems, exerts significant yet underexplored influence on heavy metal fate. Here, we systematically investigated the effect of soil DOM on cadmium (Cd) adsorption using industrial waste-synthesized zeolites, which showed high adsorption efficiency across pH 3.0–7.0. More importantly, adsorption-desorption experiments confirmed that soil DOM selectively enhanced Cd immobilization on zeolites through its functional groups. By integrating EEM-PARAFAC, FT-ICR-MS, and 2D-FTIR-COS analyses, we further identified the dominant soil DOM components and specific functional groups responsible for the immobilization. In soil DOM-free systems, Cd adsorption was primarily governed by Si/Al O bonds, whereas in soil DOM-amended systems, Cd retention was jointly controlled by zeolite surface sites and DOM-derived oxygen-containing functional groups, forming a stable zeolite-soil DOM-Cd ternary system, in which hydroxyl groups ( OH) played a dominant role in promoting Cd immobilization. This work provides new molecular-level insights into the critical role of soil DOM in enhancing heavy metal immobilization and offers a scientific basis for developing effective remediation strategies using waste-derived zeolites for treating Cd contaminated wastewater. Download: Download high-res image (145KB) Download: Download full-size image
✓ 已验证
DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2020
·
IF 3.644
Zang Yuan-Yuan,Yang Sha,Xu Yong-Qiang,Chen Zhi-Gang,Wu Tao
In this study, a strain with rutin degrading enzyme (RDE) activity was screened from moldy tartary buckwheat and subsequently identified as Fusarium spp. The structure and enzyme characteristics of CLEA-RDE formed by immobilization via cross-linking were then investigated. Further, the optimal catalysis conditions of CLEA-RDE in natural deep eutectic solvents (NADESs) serving as hydrolysis solvents were also investigated. The results of SEM and spectrum indicated that CLEA-RDE became more stable than free-RDE due to the cross-linking. Interestingly, CLEA-RDE showed a wider range of pH adaptation and higher tolerance to low temperatures (20 – 30°C) and hydrophobic environments. The results of orthogonal experiments revealed that the optimal condition for rutin hydrolysis was under pH 5.0 and 40oC with the degradation rate of 10.65 mg min−1 L−1. The preparation of CLEA-RDE without a carrier-based immobilization method reduces the loss of enzyme activity, improves the stability of the enzyme and can be applied to the investigation of immobilization of various enzymes, thus providing a referred idea for the improvement of catalysts in industrial production.
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 107
·88456-88463
·
IF 3.84
Anqiang Zhang,Liujun Cheng,Shuanghao Hong,Caixia Yang,Yaling Lin
Polydimethylsiloxane (PDMS) is a widely used material for biomedical applications. In this work, a convenient method for the covalent modification of PDMS with carboxybetaine was developed and used to construct a biocompatible and anti-fouling coating. Following the preparation of a Si–H functionalized PDMS film by adjusting the molar ratio of the two components used in Sylgard 184 silicone elastomer networks, the allyl carboxybetaine (ACB) was grafted to the PDMS surface via a hydrosilylation reaction in the presence of a Karstedt's catalyst. ATR-FTIR and water contact angle measurements revealed that carboxybetaine was introduced to the PDMS surface successfully. The biocompatibilities of PDMS and carboxybetaine-modified PDMS (PDMS-CB) films were evaluated by cytotoxicity, hemocompatibility, and dynamic clotting time. The anti-fouling properties of PDMS-CB were evaluated by protein adsorption and bacterial adhesion measurements. The results showed that the carboxybetaine layer could enhance the biocompatibility of PDMS and reduce the adsorption of protein and adhesion of bacteria efficiently.
研究领域:
生物材料
·
医学工程
✓ 已验证
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2025
·Vol. 51
·期 26
·49745-49753
·
IF 5.6
Yusen Xing,Rugeng Liu,Wei Han,Mei Li
Zircon (ZrSiO 4 ), due to its excellent thermal stability and chemical durability, is an efficient solidification matrix for high-level radioactive waste (HLW). However, the traditional solid-state method has problems such as high synthesis temperature (>1600 °C), long reaction time (>24 h), and insufficient product purity. Thus in this study, the synthesis conditions were optimized to 1400 °C/6 h by adding 10 wt% MgO as a mineralizer, significantly reducing the reaction temperature and time. The mechanism of MgO as a mineralizer was investigated. XRD revealed that MgO promoted the transformation of m-ZrO 2 (monoclinic zirconia) to t-ZrO 2 (tetragonal zirconia) and reacted with SiO 2 to form MgSiO 3 , jointly lowering the reaction temperature and time. Based on this, the lattice substitution strategy was used to immobilize Sm 3+ (simulating trivalent actinides) in Sm x Zr (1-x) SiO 4 (0 < x < 0.5) solid solutions. XRD indicated that when x ≤ 0.45, a stable coexistence phase of Sm x Zr (1-x) SiO 4 (zircon structure) and m-ZrO 2 (monoclinic zirconia) was formed, and Sm 3+ successfully replaced Zr 4+ . When x > 0.45, the structure collapsed, with peak broadening and the appearance of a second phase. The maximum component (x = 0.45) was combined with borosilicate glass to prepare glass-ceramic solidification bodies. In the 90 °C static leaching test, the 28-day normalized leaching rate of Sm 3+ ( LR Sm ) was ∼10 −6 g m −2 d −1 . This strategy provides a new solution for the long-term immobilization of fission element.
研究领域:
材料科学
·
核废料管理
·
陶瓷学
·
固态化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF SOLID STATE CHEMISTRY
·
2021
·
IF 3.498
Yaxin Feng,Guilin Wei,Yi Liu,Wenhong Han,Yan Chen,Runjie Sun,Lin Peng,Meihua Ma,Yu Zhang,Zhentao Zhang,Xirui Lu
The immobilization of iodine waste is a challenge in the field of nuclear waste . In this work, iodine waste (silver-coated silica gel with different iodine loadings) was immobilized at low-sintering temperatures (500, 550, and 600 °C). XRD represented that the crystallized phases of the sintered matrix consist of AgI and B 2 O 3 . The crystallization behavior of boron can be found as the sintering temperature increased from 500 °C to 600 °C. FT-IR results proved that in the process of crystallization, part of the microstructure related to B–O stretching transformed from [BO 4 ] to [BO 3 ] units, which benefits low-temperature iodine waste immobilization due to the bonding energy of [BO 3 ] is higher than that of [BO 4 ]. Our work reveals the B-related microstructure transition in low-temperature immobilization iodine waste.
研究领域:
核废物处理
·
材料科学
·
无机化学
·
放射化学
·
环境工程
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Jing Zhou,Dapeng Li,Shutong Chen,Yao Xu,Xue Geng,Chaoran Guo,Yong Huang
The immobilization of sedimentary phosphorus (P) by capping sediments with amended calcium peroxide material (ACPM) and CaO 2 was investigated, the sediments and overlying water from a black-odor river. Under the capping with ACPM and CaO 2 , the pH and dissolved oxygen (DO) in the overlying water were higher than in the control. This contributed to the more loss of external P under the ACPM (the phosphate sorption was fitted well by the Langmuir equation, the maximum P sorption capacity up to 29.28 mg P g −1 ). Additionally, ACPM improves the decrease of the reduction materials (e.g., NH 4 + -N, Fe 2+ ) in the pore water compared to that associated with CaO 2 , resulting in a less obvious dissolved inorganic P (DIP) in the pore water, but the accumulation of DIP in the pore water with CaO 2 suggested an explosive release once the micro-environment conditions encounters adverse conditions. The sharp reduction of Fe/Al-P and Res-P contributes to the increase of DIP in the pore water, with a release of up to 390.60 mg P kg −1 DW (dry weight), whereas the value is only 62.55 mg P kg −1 DW when capped with ACPM. Correspondingly, the percentage of the bioavailable P to Tot-P decreases rapidly from 17.27% (control, 0–2 cm) to 7.21% (ACPM, 0–2 cm) and 10.57% (CaO 2 , 0–2 cm), indicating the obvious P immobilization. The implication is that the application of CaO 2 is not suitable for direct use, while the combination with other materials (e.g., WPS) is a better choice.
研究领域:
环境科学
·
水体修复
·
环境化学
·
污染控制
·
沉积物管理
✓ 已验证
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2019
·
IF 3.218
Dou Ying,Xing Yan Xu,Chuan Bao Cao,Zhuo Chen
Although lithium sulfur batteries (LSBs) possess extremely high specific capacity and ultrahigh energy density , the low conductivity and severe shuttle effect greatly restrict its practical application. Herein, we report a facile strategy to overcome the problems via a double-layer structure, in which one provides strong chemisorption by functionalized reduced graphene oxide (rGO) while the other confines polysulfides physically through coating a layer of rGO film on the electrode. The strategy not only alleviates the dissolution and diffusion of polysulfides but also inhibits polysulfides from penetrating into anode to some extent and enhances the electrical conductivity of electrode. The LSBs employing double-layer structure electrodes exhibit high initial capacity of 1353 mAh g −1 at 0.1 C and 78% capacity retention after 100 cycles, good cycling stability with only 0.056% capacity decay per cycle at 1 C, and remarkable rate capability with 92% reversible capacity retention. The current findings manifest that the double-layer structure can effectively improve the electrochemical performance via the dual synergistic effect of both chemical interaction and physical confinement.
研究领域:
材料科学
·
储能
·
电化学
✓ 已验证
DOI
高影响因子
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
·
2014
·Vol. 61
·期 5
·603-610
·
IF 1.322
Rongrong Li,Ling Jiang,Lidan Ye,Jie Lu,Hongwei Yu
The esterase BioH from Escherichia coli was covalently immobilized onto the surface of the functional magnetic nanosupport in an oriented manner. The surface of the Fe 3 O 4 nanosupport was modified with acyl azide groups or both acyl azide groups and hydroxyl groups. The protein loading of the support was increased from 55 to 99 mg/g by an improvement in hydrophilicity, and the activity retention of the immobilized esterase on the nanosupport was improved by 40% after hydrophilic modification (30% and 70% of the free BioH, respectively). For the BioH immobilized on the hydrophilic-modified nanosupport, the recovery activity remained 80% of the original activity after 10 times of recycling. The catalytic kinetics and thermo-/pH-stability of the immobilized esterase BioH were also determined and compared with those of the free enzyme. The comparatively high activity retention, improved thermo-/pH-stability, and good reusability of the immobilized enzyme indicate that oriented covalent immobilization is an efficient method for immobilizing esterase BioH.
研究领域:
纳米生物技术
·
酶工程
·
生物催化
·
应用微生物学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）