ANALYTICAL SCIENCES
·
2022
·Vol. 39
·期 3
·313-323
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IF 1.967
Pan Yini,Wang Zhenxin,Xu Sen,Zhang Lingyi,Zhang Weibing
It has great significance in profiling specific proteins throughout for better understanding of complex pathological processes and in-depth pharmacological studies. In this work, an efficient protein profiling strategy was developed based on the specific protein–drug interaction. Sofosbuvir (SOF), as a first-line drug for the treatment of hepatitis C, was modified onto the surface of nanoparticles through stable chemical bonds to fabricate a novel magnetic separation material denoted as Fe 3 O 4 @SiO 2 @PAA@SOF. With sequence coverage as the screening parameter, nine proteins were profiled from fetal bovine serum (FBS) of which eight were liver related. Similarly, the strategy was applied to hepatocellular carcinoma (HCC) patient serum. Eight proteins were profiled and all of them were liver related, demonstrating the superb specificity and selectivity of this strategy for profiling liver-related proteins by virtue of protein–SOF interaction. When serum proteins from HCC patients were compared to those from healthy people, one unique differential protein (D3DQX7) was profiled, which was liver related and was a potential target for ameliorating liver diseases. For further research, this material design concept and protein profiling strategy can be extended to employ other drugs for corresponding studies.
Graphical abstract Sofosbuvir, as a therapeutic drug for liver diseases, was modified onto the surface of magnetic nanoparticles to fabricate the specific selective separation material (Fe 3 O 4 @SiO 2 @PAA@SOF). Based on protein–SOF interaction, the material was applied to adsorb specific proteins from different serum samples. After MS analysis, specific proteins, most of which were liver related, were successfully profiled from FBS and HCC patient serum, fully demonstrating the superb specificity and selectivity of this protein profiling strategy.
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高影响因子
Journal of Food Measurement and Characterization
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2022
·
IF 3.006
Ye Hua,Wan Tianxiang,Li Xinfu,Li Chao,He Kuo,Guo Yuanxin
Split aptamers are fragments of the parent aptamer which still possess binding ability to its target. They have shown great potential in the development of aptasensors. In this study, a fluorescence aptasensor was constructed employing the split aptamer as a cooperative recognition molecular probe and Graphene Oxide nanosheets (GO) as a quencher for rapid detection of kanamycin in chilled pork. Under optimal conditions,the proposed biosensor showed a good linear relationship in the range of 0.5–50 nM with the detection limit of 0.36 nM. The recoveries of the actual samples ranged from 97.6 to 103.5%. Furthermore, the test can be completed in 25 min. This study provides good reference for developing simple, economical, and rapid assay for other small molecular targets detection.
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Carbon Letters
·
2022
·Vol. 33
·期 2
·501-516
·
IF 3.117
Zhang Chunhong,Xu Zice,Sui Wenbo,Zang Junbo,Ao Yuhui,Wang Lu,Shang Lei
Epoxy resin (EP) is a thermosetting resin with excellent properties, but its application is limited due to its high brittleness and poor flame retardancy. Therefore, to solve this problem, a dispersion system of imidazole-containing ionic liquid ([Dmim]Es) and graphene in epoxy resin is designed based on the π–π stacking effect between imidazole and graphite layers. The study on the thermal and flame-retardant properties of the composites show that the modified [Dmim]Es–graphene nanosheets improved the flame retardancy, smoke suppression and thermal stability of epoxy resin. With the addition of 5wt% [Dmim]Es and 1% Gra, the exothermic rate (HRR) and total exothermic (THR) of the composites decrease by 35% and 30.2% compared with the untreated epoxy cross-linking, respectively. The limiting oxygen index reaches 33.4%, the UL-94 test rating reaches V-0. The characterization of mechanical properties shows that the tensile properties and impact properties increased by 13% and 30%, respectively. Through SEM observation, the addition of [Dmim]Es improves the dispersion of graphene in the EP collective and changes the mechanical fracture behavior. The results show that ionic liquid [Dmim]Es-modified graphene nanosheets are well dispersed in the matrix, which not only improves the mechanical properties of epoxy resin (EP), but also has a synergistic effect on flame retardancy. This work provides novel flame-retardant and graphene dispersion methods that broaden the range of applications of epoxy resins. Graphical abstract
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
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2022
·Vol. 30
·期 9
·23372-23385
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IF 5.19
Ji Yuanhuo,Wang Xiwen
A heavy metal ion adsorbent (HFPANF) with high surface area was obtained from polyacrylonitrile fibers with fibrillation and alkali hydrolysis, and an activated carbon fiber filter was prepared by using HFPANF as the binder. The surface area of polyacrylonitrile was 48.64 m 2 /g due to fibrillation, which also led to the carboxyl content of the HFPANF up to 3.4 mmol/g. Batch adsorption experiments on Cu 2+ and Pb 2+ showed that the adsorption capacities of HFPANF for Cu 2+ and Pb 2+ were 47.5 mg/g and 54.3 mg/g. The adsorption kinetics showed that the adsorption reached equilibrium at 90 min and that the adsorption followed the pseudo-second order model. It indicates that the adsorption process is chemisorption. HFPANF formed a single tooth chelate with Cu and a double tooth chelate with Pb. HFPANF-ACF filter was prepared by wet molding technique. When the HFPANF content was 30%, the filter reached a compressive strength of 15.37 MPa and its maximum flux was 180 L/h. 2.5 mg/L of Cu and Pb were used for dynamic adsorption experiments and the heavy metal removal rate was still above 95% after filtering 600 L. The pressure drop of HFPANF-ACF filter was much smaller compared with that of GAC filter due to the combined effect of fibrillated nanofibers and ACF, which can improve the filtration efficiency of the filter. Graphical Abstract
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MOLECULAR NEUROBIOLOGY
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2022
·Vol. 60
·期 2
·524-544
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IF 5.682
Wu Mengyu,Liao Wenli,Zhang Ruyi,Gao Yuting,Chen Tao,Hua Liangliang,Cai Fei
Accumulating clinical and epidemiological studies indicate that learning and memory impairment is more prevalent among people with diabetes mellitus (DM). PTP1B is a member of protein tyrosine phosphatase family and participates in a variety of pathophysiological effects including inflammatory, insulin signaling pathway, and learning and memory. This study was aimed to investigate the effects of CA, a specific inhibitor of PTP1B, on spatial learning and memory impairment in diabetic mice caused by high-fat diet and injection of streptozotocin. We found that the protein expressions of PTP1B increased in hippocampal CA1, CA3, and PFC regions of diabetic mice. Network pharmacology results showed that PTP1B might be one of the key targets between diabetes and cognitive dysfunction, and CA might alleviate DM-induced cognitive dysfunction. Animal experiments showed that CA ameliorated DM-induced spatial learning and memory impairment, and improved glucose and lipid metabolic disorders. Moreover, administration of CA alleviated hippocampal structure damage and enhanced the expressions of synaptic proteins, including PSD-95, SYN-1, and SYP in diabetic mice. Furthermore, CA treatment not only significantly down-regulated the expressions of PTP1B and NLRP3 inflammatory related proteins (NLRP3, ASC, Caspase-1, COX-2, IL-1β, and TNF-α), but also significantly up-regulated the expressions of insulin signaling pathway–related proteins (p-IRS1, p-PI3K, p-AKT, and p-GSK-3β) in diabetic mice. Taken together, these results suggested that PTP1B might be a targeted strategy to rescue learning and memory deficits in DM, possibly through inhibition of NLRP3 inflammasome and regulation of insulin signaling pathway.
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APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
·
2022
·Vol. 128
·期 11
·1-11
·
IF 2.983
Zhang Liang,Zhang Ran,Cui Lili,Li Tao,Gu Dayong,He Jian’an,Zhang Sixiang
The sensitization effect of graphene oxide (GO) on surface plasmon resonance (SPR) chip sensors has been widely reported. However, the absence of a fixed value for selecting the GO solution concentration is a significant limitation for reproducing the experiments reported in the literature. In this study, linear functionalized polyethylene glycol (SH-PEG-NH 2 ) is used to modify the surfaces of bare gold chips, using flow and immersion methods to produce GO-film-covered SPR sensor chips. Deionized water and phosphate buffered saline are applied as refractive index variants to compare the changes in sensor performance following chip modification, and to determine the optimal concentrations of GO solution under the respective modification methods. Using a sensor chip modified with the optimal GO concentration enhances the ability of a 10-year-old device to detect the dengue virus (DENV) E-protein by 357% and enables the detection of antibody protein concentrations as low as 62.5 ng/mL. Satisfactory results were also obtained using a DENV-positive serum to verify the chip usage. The results of this study are of significance for future efforts involving the rapid preparation of accurate and low-cost GO-SPR biosensor chips.
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Nature Communications
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2022
·Vol. 13
·期 1
·1-9
·
IF 17.694
Wang Yunhu,Wang Kunpeng,Dai Fangxu,Zhang Kai,Tang Haifeng,Wang Lei,Xing Jun
Artificial lighting consumes almost one-fifth of global electricity. As an efficient solid-state lighting technology, white light-emitting diodes (WLEDs) have received increasing attention. However, the white luminescence of the traditional WLEDs comes from multi-component emitters, which leads to complex device structure and unstable emitting color. Therefore, developing single-component materials with white-light electroluminescence is of significance for artificial lighting applications. Here, we fabricate single-component white-light electroluminescence devices based on an aromatic carbon nitride material and improve the performance of WLEDs by adjusting the carrier transport. The carbon nitride LEDs emit warm-white light, of which color coordinates and color temperature are (0.44, 0.52) and 3700 K. The optimized LEDs display a very low turn-on voltage of 3.2 V and achieve a milestone in the maximum luminance and external quantum efficiency of 1885 cd m −2 and 1.20%. Our findings demonstrate the low-cost carbon nitride materials have promising potential for single-component WLEDs application.
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Nature Communications
·
2022
·Vol. 13
·期 1
·1-11
·
IF 17.694
Xia Nan,Xing Jianpei,Peng Di,Ji Shiyu,Zha Jun,Yan Nan,Su Yan,Jiang Xue,Zeng Zhi,Zhao Jijun,Wu Zhikun
Nanoparticle assembly paves the way for unanticipated properties and applications from the nanoscale to the macroscopic world. However, the study of such material systems is greatly inhibited due to the obscure compositions and structures of nanoparticles (especially the surface structures). The assembly of atomically precise nanoparticles is challenging, and such an assembly of nanoparticles with metal core sizes strictly larger than 1 nm has not been achieved yet. Here, we introduced an on-site synthesis-and-assembly strategy, and successfully obtained a straight-chain assembly structure consisting of Ag 77 Cu 22 (CHT) 48 (CHT: cyclohexanethiolate) nanoparticles with two nanoparticles separated by one S atom, as revealed by mass spectrometry and single crystal X-ray crystallography. Although Ag 77 Cu 22 (CHT) 48 bears one unpaired shell-closing electron, the magnetic moment is found to be mainly localized at the S linker with magnetic isotropy, and the sulfur radicals were experimentally verified and found to be unstable after disassembly, demonstrating assembly-induced spin transfer. Besides, spin nanoparticles are found to couple and lose their paramagnetism at sufficiently short inter-nanoparticle distance, namely, the spin coupling depends on the inter-nanoparticle distance. However, it is not found that the spin coupling leads to the nanoparticle growth.
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CHINESE JOURNAL OF POLYMER SCIENCE
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2022
·Vol. 41
·期 1
·40-50
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IF 3.815
Wu Xin-Yu,Yang Jun,Wu Fang-Hui,Cao Wang-Bei,Zhou Tong,Wang Zhao-Yi,Tu Chen-Xi,Gou Zhong-Ru,Zhang Lei,Gao Chang-You
The dense networks of conventional hydrogel hinder the efficient cell penetration and thus tissue regeneration. In this study, a macroporous cryogel with excellent mechanical properties was designed and fabricated through cryogelation and crosslinking of methacrylated gelatin (Gel-MA) and gelatin grafted with 2-ureido-4[1 H ]-6-methyl-pyrimidinone (Gel-UPY). The successful modification of the two functional units on the side chain of gelatin was confirmed by 1 H-NMR and infrared spectroscopy. The macroporous structure of cryogel was verified by scanning electron microscopy and confocal microscopy. The compressive modulus of cryogel (110 kPa) was 2 magnitudes higher than that of the conventional hydrogel of the same chemical compositions (8 kPa). Both the hydrogel and cryogel exhibited excellent stress-recovery ability. The cryogel could well maintain the viability of chondrocytes and facilitated their migration into the scaffold interior. After being implanted into osteochondral defects in rabbits for 12 w, the Gel-MA/Gel-UPY cryogel better facilitated the regeneration of upper cartilage and subchondral bone compared to the conventional hydrogel.
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Frontiers of Optoelectronics
·
2019
·Vol. 11
·期 4
·348-359
·
IF 0
Liu Jikang,Li Junli,Tu Guoli
The electron transport layer (ETL) plays a crucial role on the electron injection and extraction, resulting in balanced charge transporting and reducing the interfacial energy barrier. The interface compatibility and electrical contact via employing appropriate buffer layer at the surface of hydrophobic organic active layer and hydrophilic inorganic electrode are also essential for charge collections. Herein, an ether chain functionalized fullerene derivatives [6,6]-phenyl-C 61 -butyricacid-(3,5-bis(2-(2-ethoxyethoxy)-ethoxy)-phenyl)-methyl ester (C 60 -2EPM) was developed to modify zinc oxide (ZnO) in inverted structure organic solar cells (OSCs). The composited ZnO/C 60 -2EPM interface layer can help to overcome the low interface compatibility between ZnO and organic active layer. By introducing the C 60 -2EPM layer, the composited fullerene derivatives tune energy alignment and accelerated the electronic transfer, leading to increased photocurrent and power conversion efficiency (PCE) in the inverted OSCs. The PCE based on PTB7-Th: PC 71 BM was enhance from 8.11% on bare ZnO to 8.38% and 8.65% with increasing concentrations of 2.0 and 4.0 mg/mL, respectively. The fullerene derivatives C 60 -2EPM was also used as a third compound in P3HT:PC 61 BM blend to form ternary system, the devices with addition of C 60 -2EPM exhibited better values than the control device.
研究领域:
材料科学
·
光电化学
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2019
·Vol. 30
·期 3
·2572-2584
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IF 2.195
Hu Fengxian,Cui Entian,Liu Hongxia,Wu Jing,Dai Yong,Yu Guiyun
Series of Bi 2 MoO 6 /NiAl-LDH hetero-junction photocatalysts were synthesized by a two-step hydrothermal method with a self-assembled process. It indicated that the in-situ intercalated NiAl-LDH showed a tight contact with Bi 2 MoO 6 layers and thus exhibited the rapid transfer of photogenerated electrons on the interface. The photocatalytic performance was evaluated by the degradation of rhodamine B (RhB) under visible light irradiation. The resulted Bi 2 MoO 6 /NiAl-LDH sample showed a higher visible-light photocatalytic activity for the degradation of RhB than any single component. The optimal LDH content in Bi 2 MoO 6 /NiAl-LDH is 15% with the degradation efficiency for more than 90% during 60 min. The excessive amount of NiAl-LDH in Bi 2 MoO 6 /NiAl-LDH could reduce the light absorption efficiency of Bi 2 MoO 6 and thus affected the generation of photoinduced electron–hole pairs. The enhanced photocatalytic activity and stability of Bi 2 MoO 6 /NiAl-LDH was attributed to the synergistic effects of the introduction of narrow band-gap NiAl-LDH and the construction of layered hetero-junction. Our synthetic method of self-assembly sheets has certain guided significance for the synthesis and application of other photocatalysts.
研究领域:
材料科学
·
环境科学
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APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
·
2019
·Vol. 125
·期 1
·1-11
·
IF 1.784
Deng Senlin,Yang Zebin,Lv Guojun,Zhu Yongqiang,Li Haichao,Wang Fumin,Zhang Xubin
A novel WO 3 nanosheets (WO 3 NS)/g-C 3 N 4 Nanosheets’ (g-C 3 N 4 NS) nanocomposite photocatalyst was fabricated through a simple calcination process. WO 3 NS was first prepared with the aid of P123 lamellar micelles and g-C 3 N 4 NS was synthesized by two-step calcination process. The nanocomposite photocatalyst was characterized by X-ray powder diffraction, scanning electron microscopy, Fourier transform infrared spectrometry, UV–Vis diffuse reflection spectroscopy, X-ray photoelectron spectroscopy, and photoluminescence. The novel nanocomposite photocatalyst demonstrated higher photocatalytic activity for rhodamine B degradation than corresponding g-C 3 N 4 NS. 20 wt% WO 3 NS/g-C 3 N 4 NS showed the highest photocatalytic performance, which was 4.70 times higher than that of pure g-C 3 N 4 NS. The results confirmed that the incorporation of WO 3 NS could definitely accelerate the transfer of photogenerated electrons and effectively promote the separation of electrons and holes, thereby improving the photocatalytic efficiency. The trapping experiments proved that the mechanism of photocatalytic degradation may be rooted in the formation of Z-scheme system.
研究领域:
材料科学
·
环境科学
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