Stem Cell Research & Therapy
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2021
·Vol. 12
·期 1
·1-16
·
IF 6.832
Zhou Ping,Shi Jia-Min,Song Jing-E,Han Yu,Li Hong-Jiao,Song Ya-Meng,Feng Fang,Wang Jian-Lin,Zhang Rui,Lan Feng
Background Derivation of osteoblast-like cells from human pluripotent stem cells (hPSCs) is a popular topic in bone tissue engineering. Although many improvements have been achieved, the low induction efficiency because of spontaneous differentiation hampers their applications. To solve this problem, a detailed understanding of the osteogenic differentiation process of hPSCs is urgently needed. Methods Monolayer cultured human embryonic stem cells and human-induced pluripotent stem cells were differentiated in commonly applied serum-containing osteogenic medium for 35 days. In addition to traditional assays such as cell viability detection, reverse transcription-polymerase chain reaction, immunofluorescence, and alizarin red staining, we also applied studies of cell counting, cell telomerase activity, and flow cytometry as essential indicators to analyse the cell type changes in each week. Results The population of differentiated cells was quite heterogeneous throughout the 35 days of induction. Then, cell telomerase activity and cell cycle analyses have value in evaluating the cell type and tumourigenicity of the obtained cells. Finally, a dynamic map was made to integrate the analysis of these results during osteogenic differentiation of hPSCs, and the cell types at defined stages were concluded. Conclusions Our results lay the foundation to improve the in vitro osteogenic differentiation efficiency of hPSCs by supplementing with functional compounds at the desired stage, and then establishing a stepwise induction system in the future.
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高影响因子
CATALYSIS LETTERS
·
2021
·Vol. 151
·期 8
·2291-2301
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IF 3.186
Fan Xizheng,Yang Jingyi,Pang Qingqing,Liu Zhongyi,Zhang Panke,Yang Jing-He
Abstract Construction of heterogeneous Pd/SiO 2 catalyst via the pollution-free strategy marked strong electrostatic adsorption has been reported for the application to Suzuki–Miyaura cross-coupling reactions. The exposed negatively charged oxygen groups, which were converted from the hydroxyl groups on the surface of silica under the alkaline atmosphere, could effectively anchor palladium species to form ultrafine Pd nanoparticles (Pd NPs) with an average particle size of 1.3 nm and high dispersion (43%). Pd/SiO 2 catalyst was endowed with the excellent catalytic performance which was that the yield of the Suzuki–Miyaura reaction between bromobenzene and phenylboronic acid at 40 °C was > 99% for 30 min and the TOF was ~ 80,000 h −1 . The catalyst could be easily recovered and recycled by facile procedure without a significant decrease in catalytic activity, which was able to maintain the 90% yield after repeated for 8 times. In addition, a continuous flow reaction device was designed using the Pd/SiO 2 catalyst to effectively improve the production efficiency of biphenyl. Graphic Abstract Pd/SiO 2 catalyst constructed with the strategy of strong electrostatic adsorption (SEA) possesses uniformly dispersed and highly exposed Pd sites which can be easily transformed into electron-deficient Pd δ+ and strengthened stability for itself due to its strong interaction with the front surface of the carrier, and has been endowed the outstanding catalytic performance for Suzuki − Miyaura cross-coupling reaction.
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JOURNAL OF MATERIALS ENGINEERING AND PERFORMANCE
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2021
·Vol. 30
·期 2
·1567-1576
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IF 1.819
Ma Jingling,Jia Xingliang,Ma Mingsheng,Zhang Chenfei,Ren Fengzhang,Wang Guangxin
To limit corrosion and retain activity of magnesium anode, magnesium–air batteries with double solutions: 0.5 M Mg(ClO 4 ) 2 - N , N -dimethylformamide (DMF)/0.6 M NaCl-H 2 O, 0.5 M Mg(ClO 4 ) 2 –acetonitrile (AN)/0.6 M NaCl-H 2 O are prepared. Cathodic and anodic reactions proceed in inorganic and organic solutions separately. Discharge performance, discharge morphology, and corrosion behavior of magnesium anode are researched. Results obtained show that organic electrolytes separate anode from the aqueous electrolyte, thus improving anode utilization rate. Due to 0.6 M NaCl solution used in air cathode side, the batteries show higher discharge voltages. A better discharge performance has been observed in double electrolytes at 1 mA·cm −2 discharge. Results of discharge morphology and electrochemical impedance spectroscopy agree well with discharge performance. The discharge mechanism of magnesium anode in different solutions is also discussed.
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JOURNAL OF NANOPARTICLE RESEARCH
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2021
·Vol. 23
·期 1
·1-12
·
IF 2.253
Zhang Gongguo,Liu Zhiang,Fu Xiaowei,Zhao Wenjun,Yin Xunxiao,Niu Xiankang,Kong Rongmei,Qu Fengli,Zheng Yiqun
Branched Au nanocrystals with sub-10-nm arms are synthesized via a one-pot reduction in aqueous phase. The success of current work relies on the use of octadecyltrimethylammonium chloride as the shape-directing capping agent to facilitate the formation of branched products in high purity. Their overall size could be controlled in the range of 16–60 nm by varying the Au precursor amount or concentration of capping agent, and the diameter of arms is limited to sub-10-nm size range. Interestingly, Au nanocrystals with a unique morphology, sheet-wrapped dendrites, could be obtained when the concentration of a reductant was varied. The current strategy is also validated effective in the fabrication of dendritic products composed of other noble metals or alloys in addition to Au by simply using the alternative metal precursor. As compared to spherical counterparts, the current branched Au nanocrystals exhibited noticeable red-shift in UV-Vis extinction spectrum and enhanced catalytic activities for electrooxidation of ethanol in alkaline media. Graphical abstract
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POLYMER BULLETIN
·
2021
·Vol. 79
·期 1
·443-462
·
IF 2.87
Wang Chuanfeng,Chen Yingbo,Yang Kang,Hu Xiaoyu,Zhang Yufeng
In this study, a graphene oxide/polyvinylidene fluoride (GO/PVDF) tight hollow fiber membrane (HFM) with improved permeability was fabricated via one-step direct spinning process to fractionate the dyes and salts (NaCl and Na 2 SO 4 ) in textile wastewater. Scanning electron microscopy (SEM) indicated that the GO/PVDF HFM possessed a smooth and tight outer selective layer and a relatively porous and loose inner surface. The optimized HFM not only possesses a small mean pore radius of 1.14 nm, but also has a high pure water flux of 24.8 L/m 2 ·h·bar. The stronger negative charge (− 40 mV at pH = 7) was obtained by the GO/PVDF HFM. Due to the synergistic effects of size exclusion and charge repulsion, the resultant HFM exhibited excellent rejection for dyes (i.e., close to 100% for Congo red and Methyl blue, 98.9% for Eriochrome black T) and low salts rejection (i.e., 1% for NaCl, 11% for Na 2 SO 4 ). Besides, with appropriate addition of hydrophilic GO to the doped solution, the resultant membrane exhibited good hydrophilicity, antifouling property and excellent stability. The newly developed tight HFM would be of great significance for effective fractionation and treatment of textile wastewater.
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JOURNAL OF MATERIALS SCIENCE
·
2020
·Vol. 56
·期 5
·3755-3777
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IF 3.553
Huang Haoxin,Zhang Huiping,Yan Ying
Abstract Three types of carbon nanotubes coated on paper-like sintered stainless steel fibers (PSSF) were synthesized and applied for continuous catalytic wet hydrogen peroxide oxidation (CWPO) of phenol. The novel metal-free nitrogen-doped carbon nanotube-coated PSSF catalyst (N-CNTs/PSSF) was designed by direct chemical vapor deposition using melamine as solid precursor for the first time, and CNTs/PSSF and Fe 2 O 3 -deposited CNTs/PSSF (Fe-CNTs/PSSF) catalysts were synthesized for comparison. Characterization results showed that all the catalysts exhibited three-dimensional networks structure, with nanotubes wrapping on the stainless steel fibers successfully, and the N-CNTs showed distinct irregular corrugated morphology with nitrogen-doping level about 5.18 at.%. CWPO of phenol revealed that the N-CNTs/PSSF catalyst achieved favorable catalytic activity and highest stability during 100-h reaction (phenol and TOC conversions reached about 90% and 50%, respectively), while phenol conversions of CNTs/PSSF and Fe-CNTs/PSSF catalysts decreased over time to 72% and 58%, respectively. The N-CNTs/PSSF catalyst also showed the lowest Fe weight loss ratio (about 2.44 wt%) among the three catalysts. The favorable catalytic results of N-CNTs/PSSF can be ascribed to the synergistic effects of the strong affinity toward polar molecules of N-CNTs, as well as the enhancement in contact efficiency associated with three-dimensional networks structure of the PSSF. Graphical abstract
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Nature Communications
·
2020
·Vol. 11
·期 1
·1-9
·
IF 12.121
Li Hongdong,Han Yi,Zhao Huan,Qi Wenjing,Zhang Dan,Yu Yaodong,Cai Wenwen,Li Shaoxiang,Lai Jianping,Huang Bolong,Wang Lei
Designing electrocatalysts with high-performance for both reduction and oxidation reactions faces severe challenges. Here, the uniform and ultrasmall (~3.4 nm) high-entropy alloys (HEAs) Pt 18 Ni 26 Fe 15 Co 14 Cu 27 nanoparticles are synthesized by a simple low-temperature oil phase strategy at atmospheric pressure. The Pt 18 Ni 26 Fe 15 Co 14 Cu 27 /C catalyst exhibits excellent electrocatalytic performance for hydrogen evolution reaction (HER) and methanol oxidation reaction (MOR). The catalyst shows ultrasmall overpotential of 11 mV at the current density of 10 mA cm −2 , excellent activity (10.96 A mg −1 Pt at −0.07 V vs. reversible hydrogen electrode) and stability in the alkaline medium. Furthermore, it is also the efficient catalyst (15.04 A mg −1 Pt ) ever reported for MOR in alkaline solution. Periodic DFT calculations confirm the multi-active sites for both HER and MOR on the HEA surface as the key factor for both proton and intermediate transformation. Meanwhile, the construction of HEA surfaces supplies the fast site-to-site electron transfer for both reduction and oxidation processes.
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Journal of Nanostructure in Chemistry
·
2021
·Vol. 11
·期 3
·353-366
·
IF 6.391
Zhang Mengmeng,Zhao Wenjing,Zhang Xia,Li Zhiwei,Yu Laigui,Li Xiaohong,Zhang Zhijun
Abstract With the development of modern engineering technology, the development of high-performance and multifunctional polymer materials has been promoted. In this work, a novel nanohybrid based on chitosan engraved zinc and iron mixed oxyhydroxide at the presence of halloysite nanotubes (HNTs) as the carrier, followed by surface capping with stearic acid to obtain super-hydrophobic HNT@CSZFMOH nanofiller. The as-obtained HNT@CSZFMOH nanofiller was incorporated into epoxy resin (EP) matrix. With the addition of 3 wt.% HNT@CSZFMOH, the peak heat release rate and peak smoke production of the EP nanocomposite decreased by 35% and 32%, respectively. This excellent flame retardancy is attributed to that the enhanced physical barrier derived from HNT@CSZFMOH, and the dense, highly graphitized char layer formed by catalytic charring of ultrafine FeOOH and ZnOOH nanoparticles. Meanwhile, the nanohybrid imparted outstanding hydrophobicity to the hydrophilic substrate EP with the water contact angle (CA) of 159°. EP-matrix nanocomposite exhibited desired self-cleaning performance as well as good resistance against corrosive liquids (acid and alkaline solutions) and ultraviolet irradiation. The present approach, with simple procedures and desired environmental acceptance, could provide feasible pathway to fabricating multifunctional polymer–matrix composites. Graphic Abstract
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Food Analytical Methods
·
2021
·Vol. 14
·期 4
·814-822
·
IF 3.366
Chen Yisheng,Huang Caihong,Xu Xueming
In recent years, there was increasing concerns on food safety issues associated with fluorescent whitening agents from package materials. Here, we reported the development of a facile and reliable method suitable for high-throughput quantification and identification of two common fluorescent whitening agents (FWA 184 and FWA 367) in cereal products (wheat and rice flour), platformed by high-performance thin-layer chromatography. First, the sample preparation and cleanup were rapidly accomplished via an optimized solid–liquid extraction. The extracts and reference standard were simultaneously separated on silica gel plates, using the mixture of toluene and ethyl acetate (10/0.3, v/v) as the mobile phase. Then, rapid quantification was performed with densitometry in fluorescent mode (365
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JOURNAL OF POROUS MATERIALS
·
2020
·Vol. 28
·期 2
·487-493
·
IF 2.183
Feng Lijuan,Qiu Lu,Wang Shunli,Chang Zhaosen,Lu Yan,Dong Anwang,Chen Qi,Sui Zhuyin
The direct conversion of tremendous greenhouse gas methane into various value-added chemical products or energy fuels is not only a concern associated with an economic interest but also related to environmental protection. However, neither industry nor academia has an effective or low-cost way to directly achieve this result. Herein, a high specific area (962 m 2 g −1 ) porous organic polymer was designed and used as a substance to support catalytic active sites, including PdBr 2 , Pd(OAc) 2 , and well-dispersed AuPd bimetallic nanoparticles which were reduced via hydrogen flow. The as-synthesized catalysts (HNU-2 and HNU-3) can not only maintain the porous and stable structure of their pristine matrix but also show extremely high catalytic performance in methane direct transformation systems for preparing methanol derivative methyl trifluoroformate. Our simple strategy provides a complementary alternative to application of methane via an efficient one-step conversion procedure in order to reduce its impact on climate change and obtain value-added chemicals.
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CURRENT MICROBIOLOGY
·
2020
·Vol. 78
·期 1
·383-388
·
IF 1.746
Zhou Jie,Chen Jianhao,Ma Jieyu,Xu Ning,Xin Fengxue,Zhang Wenming,Zhang Hao,Dong Weiliang,Jiang Min
A Gram-staining-negative, aerobic, non-motile, rod-shaped bacterium with degradation ability of chitin, designated strain YD-1 T , was isolated from landfill soil sample collected in Wenzhou, Zhejiang province, China. The growth of strain YD-1 T occurred optimally in the tryptone soy broth (TSB) with 1.0% NaCl at pH 7.0–8.0, 30 °C. Ubiquinone-8 (Q-8) was the predominant quinone. The polar lipids of strain YD-1 T consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, five glycolipids and four lipids. The major fatty acids were iso-C 15:0 (30.7%), iso-C 17:1 ω9c (23.2%), iso-C 11:0 (18.9%), iso-C 11:0 3-OH (6.8%) and iso-C 17:0 (5.9%). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain YD-1 T was affiliated to the genus Luteimonas with the highest similarity to Luteimonas marina KCTC 12327 T (97.3%), followed by Luteimonas aquatica DSM 22088 T (96.5%) and Luteimonas composti CCUG 53595 T (96.4%). The genomic DNA G + C content of strain YD-1 T was 71.8 mol%. Average nucleotide identity (ANI) and the digital DNA–DNA hybridizations (dDDH) for draft genomes between strain YD-1 T and Luteimonas marina KCTC 12327 T were 82.7% and 26.1%, respectively. On the basis of genotypic, phenotypic and chemotaxonomic data, strain YD-1 T is considered to represent a novel species to degrade chitin in the genus Luteimonas , for which the name Luteimonas wenzhouensis sp. nov. is proposed, with YD-1 T (= KCTC 72425 T = CCTCC AB 2019153 T ) as the type strain.
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Reaction Kinetics Mechanisms and Catalysis
·
2020
·Vol. 132
·期 1
·417-429
·
IF 1.52
Xie Qi,Miao Changxi,Lei Tianqi,Hua Weiming,Yue Yinghong,Gao Zi
Dehydrogenation of ethane assisted by CO 2 has been investigated over Au catalyst supported on Y-doped CeO 2 nanorod. The catalysts were characterized by XRD, TEM, N 2 adsorption, XPS, TG, H 2 -TPR and Raman spectroscopy. Enhanced activity can be obtained after the proper Y doping. The optimal catalyst Au/Ce 0.9 Y 0.1 exhibits an ethane conversion of 20.9% and nearly 100% selectivity to ethylene without any evident tendency of deactivation in 50 h. The reducibility of surface oxygen species is found to play a key role in the dehydrogenation assisted by CO 2 , which can be improved by proper Y doping into CeO 2 nanorod. The promoting effect of CO 2 on activity is evident over Au/Ce 0.9 Y 0.1 catalyst, due to the quick transformation of H 2 product into H 2 O by the reverse water gas shift reaction. The catalyst stability is also improved by the addition of CO 2 , attributed to the elimination of coke by Bouduoard reaction.
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