SCIENCE OF THE TOTAL ENVIRONMENT
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2019
·
IF 5.589
Pengfei Xu,Hao Du,Xin Peng,Yu Tang,Yaoyu Zhou,Xiangyan Chen,Jia Fei,Yong Meng,Lu Yuan
Remediation of polycyclic aromatic hydrocarbons (PAHs) in oily sludge has become the focus of attention. UV spectrophotometer analysis showed that four types of PAHs were found in sample, which including phenanthrene, anthracene, benzo( a )anthracene and benzo( b )fluoranthene. In order to degrade PAH effectively, the laccase reverse micelles system was proposed. The system protects laccase from being affected by organic phase. Reverse micelles were prepared by using isooctane to simulate oil. The optimum water content W 0 was 10 by measuring the electrical conductivity of the system. Under this condition, the effects of pH, temperature and ionic strength on the degradation rate of PAHs were investigated. Also, compared with that of non-immobilized laccase, the ratio between the secondary structures of laccase under different conditions was studied. The results showed that the highest laccase activity was obtained at pH 4.2 and 30 °C with 60 mmol/L KCl. Meanwhile, the structure of α-helix accounts for the largest proportion, and the ratio of α-helix in the laccase secondary structure in the laccase-reverse micelle system was higher than that of the non-immobilized one under this condition. Finally, predicting the reactive site of the degradation of polycyclic aromatic hydrocarbons was simulated by ORCA (Version 4.2.0). The application in oily sludge was further conducted. This study provides an effective method and basis for the degradation of PAHs in oily sludge.
研究领域:
环境生物技术
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污染生态学
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酶工程
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有机污染物降解
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土壤修复
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FOOD CONTROL
·
2025
·
IF 6.3
Fen Tang,Fei Ma,Du Wang,Mengxue Fang,Liangxiao Zhang,Peiwu Li,Li Yu
Aflatoxin B 1 (AFB 1 ) in peanut meal poses a significant risk to human and animal health, as well as to food safety and the feed industry. However, none of the detoxification technologies demonstrate significant potential for industrial applications in terms of efficacy, cost-effectiveness, and safety for detoxifying AFB 1 in peanut meals. To address this challenge, this study proposes a novel detoxifier by integrating attapulgite (ATP), a low-cost AFB 1 adsorbent, with laccase, an effective enzyme for AFB 1 degradation. By optimization of the synthesis conditions, including the initial laccase activity and volume, reaction temperature and time, as well as the pH of the buffer system, the modified ATP-supported laccase (MATP-La) with the highest loading capacity and enzymatic activity has been successfully developed. Stability experiments revealed that the immobilized laccase considerably improved the enzymatic stability compared to free laccase. The detoxification performance of MATP-La was evaluated across a broad range of AFB 1 concentrations. Results showed that the synergistic effect between MATP adsorption and laccase-facilitated degradation enabled MATP-La to achieve excellent detoxification efficiency. Specifically, adding only 0.1% of MATP-La to contaminated peanut meal resulted in over 90% AFB 1 detoxification within 24 h. Additionally, cytotoxicity experiments demonstrated the safety of MATP-La. In summary, this study not only offers a novel and practical approach for detoxifying AFB 1 from feeds but also effectively enhances the application potential of these materials.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2026
·
IF 5.4
Xiaolu He,Yanbao Zhao,Yuting Zhang
Smart environment-responsive nanopesticides hold great potential for enhancing foliar retention and utilization efficiency. We developed a pH laccase dual-responsive delivery system DFCS using flower-like silica nanocarriers FNCs modified with carboxymethyl chitosan and sodium lignosulfonate for dinotefuran delivery. The nanocarriers unique rough flower-like morphology and hydrophilic modification enabled strong foliar adhesion. DFCS achieved a high loading capacity of 39 %. The carboxymethyl chitosan sodium lignosulfonate coating served as a dual-functional barrier endowing excellent UV stability with a UV half-life of 254 min nearly four times that of commercial DNF SC and superior rainfall resistance with 45 % washout on Shanghai Green leaves versus 64 % of conventional formulations. DFCS exhibited significant insecticidal activity against Plutella xylostella with over 85 % mortality retained after 120 min of UV irradiation. This work proposes a novel nanoformulation strategy to improve pesticide efficiency and reduce environmental loss through intelligent adhesion-enhancing and dual-responsive design.
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JOURNAL OF HAZARDOUS MATERIALS
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2026
·
IF 10.6
Like Wang,Bo Liu,Shuheng Wei,Yongkang Xi,Shouwei Yin
The development of enzyme carriers capable of concurrently enriching hydrophobic pollutants and facilitating efficient aqueous-phase biocatalysis is crucial for advanced wastewater treatment. Conventional inorganic mesoporous supports, with their uniformly hydrophilic or hydrophobic surfaces, are fundamentally limited in achieving this dual function. To address this challenge, we engineered a zein-based mesoporous particle (ZMP) that features intrinsic hydrophilic–hydrophobic partitioned structure, further stabilized by a TA/Fe 3 + coordination coating. This design creates a robust platform for high-performance enzyme immobilization, exhibiting an excellent turnover frequency. Specifically, the hierarchical architecture, comprising hydrophobic inner domains and hydrophilic outer surfaces, enables the selective adsorption of nonpolar pollutants while providing a favorable microenvironment for immobilizing hydrophilic enzyme. The TA/Fe 3+ network enhances enzyme retention without compromising substrate permeability. During phenol removal, the TA/Fe 3+ -coated ZMP-immobilized enzyme exhibited a 2.1-fold increase in the reaction rate constant and higher substrate affinity compared to the uncoated system. Furthermore, the spatially partitioned ZMP exhibited stronger hydrophobic adsorption and superior catalytic performance versus conventional mesoporous silica carriers, maintaining over 90% naphthalene removal efficiency even in complex, multi-pollutant systems. These findings highlight the structural advantages of zein-based mesoporous carriers in achieving efficient, selective, and stable biocatalysis under complex environmental conditions.
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PubMed
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FOOD HYDROCOLLOIDS
·
2023
·
IF 10.7
Lili Zheng,Minghan She,Binling Ai,Yang Yang,Xiaoyan Zheng,Shenwan Wang,Dao Xiao,Zhiguo Jiang,Zhanwu Sheng
An amyloid fiber-ferulic acid/chitosan-ferulic acid (AF-FA/CS-FA) double network hydrogel (DN) was synthesized in two steps after prefabricating micronetworks. Ferulic acid (FA) and laccase were used to form protein and polysaccharide micronetworks, respectively. Then, AF-FA and CS-FA were induced to form covalent cross-links using genipin (GP). The phase penetration behavior of the two biopolymers in different proportions could form a homogeneous microstructure. The higher structural complexity and mechanical integrity brought by this structure may be the main reason that it improved the controlled release ability of the gels. The results from a loading capacity experiment and infrared spectra showed that the DN had acquired the AF-FA network to provide a higher load rate (9.50%), possibly because adding the polysaccharide network had increased the proportion of β-sheet structures beneficial to loading FA. The DN hydrogel containing 0.7 wt% CS-FA (DN70) showed the most tightly ordered combination of protein and polysaccharide, which had the highest storage modulus (2552.82 Pa) and the lowest solubility (91.00%). The thermal and photodegradation constants of DN70 were reduced by 75.00% and 58.29%, respectively, compared to the FA monomer . The formation of advanced glycation end products (AGEs) was reduced by 44.75% in the cake with the addition of 0.5% (w/w) DN70. These results suggest that the AF-FA/CS-FA DN can improve the range of FA applications in food products and provide an attractive strategy for polyphenols as AGEs inhibitors in baked goods.
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Coatings
·
2022
·Vol. 12
·期 11
·1734
·
IF 3.236
Jiaxin Zhao,Xiaoxiao Wu,Xushuo Yuan,Xinjie Yang,Haiyang Guo,Wentao Yao,Decai Ji,Xiaoping Li,Lianpeng Zhang
Cellulose is one of the most abundant, widely distributed and abundant polysaccharides on earth, and is the most valuable natural renewable resource for human beings. In this study, three different particle sizes (250, 178, and 150 μm) of Neosinocalamus affinis cellulose were extracted from Neosinocalamus affinis powder using bio-enzyme digestion and prepared into nanocellulose (CNMs). The cellulose contents of 250, 178, and 150 μm particle sizes were 53.44%, 63.38%, and 74.08%, respectively; the crystallinity was 54.21%, 56.03% and 63.58%, respectively. The thermal stability of cellulose increased gradually with smaller particle sizes. The yields of CNMs for 250, 178, and 150 μm particle sizes were 14.27%, 15.44%, and 16.38%, respectively. The results showed that the Neosinocalamus affinis powder was successfully removed from lignin, hemicellulose, and impurities (pectin, resin, etc.) by the treatment of bio-enzyme A (ligninase:hemicellulose:pectinase = 1:1:1) combined with NH3·H2O and H2O2/CH3COOH. Extraction of cellulose from Neosinocalamus affinis using bio-enzyme A, the smaller the particle size of Neosinocalamus affinis powder, the more cellulose content extracted, the higher the crystallinity, the better the thermal stability, and the higher the purity. Subsequently, nanocellulose (CNMs) were prepared by using bio-enzyme B (cellulase:pectinase = 1:1). The CNMs prepared by bio-enzyme B showed a network structure and fibrous bundle shape. Therefore, the ones prepared in this study belong to cellulose nanofibrils (CNFs). This study provides a reference in the extraction of cellulose from bamboo using bio-enzymes and the preparation of nanocellulose. To a certain extent, the utilization of bamboo as a biomass material was improved.Keywords:Neosinocalamus affinis;cellulose;nanocellulose;bio-enzymes;particle size
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CELLULOSE
·
2022
·Vol. 29
·期 16
·8919-8935
·
IF 6.123
Fan Shuang,Chang Wei,Fei Cheng,Zhang Zhongguo,Hou Bingbing,Shi Zhuxuan,Wang Huixin,Hui Yuchen
A wearable self-powered biosensor, fabricated from a stretchable and bendable textile matrix, senses glucose in urine. This textile matrix is made of cellulose fibers. A porous three-dimensional electrode with high flexibility, conductivity, and electroactive surface area is obtained by coating multi-wall carbon nanotubes and reduced graphene oxides onto a textile matrix based on cellulose fibers. After immobilizing glucose oxidase and laccase by the polymer embed method, the bioelectrodes show good enzymatic loading density and high electrocatalytic efficiency. The relative self-powered biosensor has a good linear response to glucose in the concentration range from 0 to 40 mM (R 2 = 0.995), with a satisfactory limit of detection at 3.95 mM, owing to the high electrocatalytic efficiency of the bioelectrodes. The biosensors have good selectivity, repeatability, and long-term storage stability, exhibit excellent stretching and bending performance, and show stability to a variety of pH, because of the catalytic specificity and tolerance of enzymes, the reasonable design of the biosensor, and the stable catalytic microenvironment. The recovery of the biosensor integrated with a diaper for glucose in artificial urine samples is between 95.6 and 105.9%, suggesting a promising determination for real samples. Graphical abstract
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
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2021
·
IF 5.816
Yuchen Hui,Huixin Wang,Wei Zuo,Xiaoyan Ma
A spider nest shaped multi-scale three-dimensional substrate consisting of reduced graphene oxide (RGO) and nickel foam is fabricated for enzymatic electrodes and biofuel cells. According to the excellent conductivity and large electroactive surface area of this special structure, the enzymatic electrodes show large enzymatic loading density, low electron transfer resistance and high electrocatalytic efficiency. The strong forces in the spider nest shaped structure and the excellent enzymatic embedding method ensure the stability of the glucose oxidase bioanodes and laccase biocathodes. The Michaelis–Menten constant value for the bioanodes to glucose is calculated to be 2.24 mM, which is close to the Michaelis–Menten constant for free glucose oxidase, implying a remarkably high enzymatic activity . Employed the obtained bioelectrodes with great properties, the relative glucose/oxygen biofuel cell has an open-circuit voltage of 0.70 V, with a high output power performance, and a maximum output power of 7.05 ± 0.05 mW cm −2 , owe to the high enzyme loading and low electron transfer resistance of the electrode based on the spider nest shaped structure. After 60 days of periodic storage experiments, the performance of the biofuel cell still maintained 84.2%, showing a good long-term stability.
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Nano-Micro Letters
·
2020
·Vol. 12
·期 1
·1-12
·
IF 12.264
Xu Weiqing,Kang Yikun,Jiao Lei,Wu Yu,Yan Hongye,Li Jinli,Gu Wenling,Song Weiyu,Zhu Chengzhou
The two functional groups (nitro and amino) were introduced into MIL-101(Fe) for tuning the atomically dispersed metal active sites.
Benefiting from both geometric and electronic effects, the nitro-functionalized MIL-101(Fe) shows a superior electronic structure of active sites and low reaction energy barrier for the HO* formation.
Nitro-functionalized MIL-101(Fe)-based biosensor was successfully employed to detect acetylcholinesterase activity and organophosphorus pesticide.
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PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2020
·Vol. 44
·期 17
·7042-7050
·
IF 3.288
Jingjing Yuan,Lin Wang,Bo Xu,Ping Wang,Jiugang Yuan,Yuanyuan Yu,Chao Deng,Qiang Wang
Silk fibroins (SFs) have many attractive properties; however, the practical applications of pure fibroin materials are severely restricted by their low elasticity. To improve flexibility of regenerated SF membranes, carboxyl-terminated polyethylene glycol (cPEG) was introduced into fibroin chains using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS). Subsequently, laccase-mediated graft polymerization of polypyrrole (PPy) onto the SF-g-cPEG membrane was performed to achieve electrical conductivity. The results implied that cPEG was successfully grafted onto the SF chains; the elongation at break of the SF-g-cPEG membrane increased by three times compared to that of the untreated one. Meanwhile, the introduced cPEG promoted the binding of pyrrole radical cations onto fibroin membrane, resulting in an efficient deposition of a PPy conductive layer during the laccase treatment. Conductivity of the membrane of SF-g-cPEG/PPy meets the standard of conductive materials even after 48 cycles of bending testing. In addition, the composite membrane has acceptable in vitro biocompatibility. The present work provides a novel alternative for fabrication of flexible and conductive SF biomaterials, which would expand the applications of fibroin materials in wearable electronics.
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JOURNAL OF APPLIED ELECTROCHEMISTRY
·
2023
·
IF 2.9
Xia Zunbin,Zuo Wei,Li Haiying,Qiu Liewei,Mu Ruihua,Wang Qian,Liu Hongchen,Wang Huixin,Hui Yuchen
Herein, a self-powered lactate biosensor (SPLBs) was assembled to detect lactate in sweat based on flexible textile matrix enzymatic electrodes. A porous three-dimensional electrode with high flexibility and electrical conductivity was obtained by decorating composites of reduced graphene oxide and carboxylate multiwalled carbon nanotubes onto a cellulose fabric substrate. The excellent enzyme embedding method using a gel electrolyte showed a Michaelis–Menten constant of 1.46 mM, indicating the high enzymatic activity of lactate dehydrogenase. Moreover, the assembled (SPLBs) displayed a sensitivity of 3.16 µW mM −1 cm −2 with a linear range of 0–10 mM and a detection limit of 9.49 µM. Additionally, the biosensor has good tensile flexural stability, selectivity, and long-term stability. When integrated into clothing, the (SPLBs) recovered 91.5–102.3% of lactate from real sweat, with a relative standard deviation of less than 4.16%. This biosensor is promising for sensing lactate in human sweat. Graphical abstract
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CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Guolin Zheng,Zhe Jiang,Yifan Cui,Man Zhou,Yuanyuan Yu,Ping Wang,Qiang Wang
Enzymes, as green catalysts with high activity and substrate specificity, have attracted extensive interest in contrast to chemical catalysts with non-selectivity. However, the enzymes' activities are sensitive to external environments. Therefore, highly efficient strategies are encouraged to modulate the enzymatic activity and stability at the molecular level. Compared with conventional magnetic field, temperature, and pH-responsive enzymatic activities, the near-infrared (NIR) light-controlled activities have noninvasive and convenient controllability advantages, which remains a significant challenge. Besides, NIR light also possesses intense penetration and low phototoxicity compared with UV and blue lights. Herein, the supersmall platinum nanoparticles (Pt-NPs) were for the first time deposited on the laccase body (labelled as laccase-Pt), with the reduction of chloroplatinic acid hexahydrate by sodium borohydride. Fortunately, the synergetic catalysis between laccase and Pt-NPs was demonstrated. Moreover, with and without NIR light irradiation, laccase-Pt activities are 2.439 and 2.258 times of free laccase, respectively. NIR laser could also manipulate the reversible enzyme activity of laccase-Pt. Notably, the laccase-Pt displays a significant advance in three-dimensional structure compared with conventional immobilized and encapsulated enzymes. Then, the high-performance laccase-Pt was used as a sensitive biosensor to detect ascorbic acid, with a detection limit of 3.7 μM obtained by colorimetric approach and smartphone. Briefly, this work provides a novel and feasible idea for manipulating laccase activity by NIR laser, inspiring more scholars to control other natural enzyme activities. Simultaneously, it also gives clear proof for a better understanding of the component-activity relationship of the enzymes-metal nano-bio hybrid catalysts.
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