APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2022
·Vol. 106
·期 7
·2677-2688
·
IF 5.56
Li Jintao,Duan Yingce,Hu Ziyi,Yang Fan,Wu Xiangli,Zhang Ruiying
Abstract Lentinula edodes is one of the most important commercially cultivated edible mushrooms. It is well known that gypsum (CaSO 4 ·2H 2 O) supplementation in sawdust medium increases the yield of L. edodes , while the physiological mechanisms remain unclear. Our previous study showed that the acidification of the medium to pH 3.5–4.0 was essential for the growth of L. edodes . In this study, it was found that the oxalic acid excreted by L. edodes was responsible for the acidification of the medium. The biosynthesis of oxalic acid was regulated by the ambient pH and buffer capacity of the medium. To acidify the sawdust medium, the concentrations of total and soluble oxalate were 51.1 mmol/kg and 10.8 mmol/kg, respectively. However, when the concentration of soluble oxalate was 8.0 mmol/kg, the mycelial growth rate decreased by 29% compared with the control. Soluble oxalate was toxic to L. edodes , while soluble sulfate was nontoxic. CaSO 4 reacted with soluble oxalate to form nontoxic insoluble CaC 2 O 4 and the strong acid H 2 SO 4 . When the CaSO 4 supplemented in sawdust medium was more than 25 mmol/kg, the soluble oxalate decreased to less than 1 mmol/kg, and the mycelial growth rate increased by 32% compared with the control. In conclusion, gypsum improved the growth and yield by relieving the toxicity of oxalate and facilitating the acidification of sawdust medium. Key points L. edodes excretes oxalic acid to acidify the ambient environment for growth. Soluble oxalate is toxic to L. edodes. Gypsum increases growth by reacting with oxalate to relieve its toxicity. Graphical abstract
研究领域:
真菌学
·
植物生理学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2016
·Vol. 163
·期 10
·A2240
·
IF 3.014
Cheng Ma,Yuehong Shu,Hongyu Chen
An environmental friendly recycling process is investigated to recover lead as leady oxide from spent lead-acid battery paste. In this study, sodium oxalate as a desulfurizer and oxalate acid as a reducing agent have been used to leach three major components (PbSO4, PbO2 and PbO) respectively to obtain the optimal reaction conditions for real industrial spent lead paste leaching. In this sodium oxalate and oxalate acid leaching system, no extra reducing agent was needed any more to reduce PbO2. The results show that sodium oxalate exhibits much higher desulfurization efficiency for PbSO4, and PbO2 can be reduced easily in oxalate acid solution under the optimum conditions with an ratio of 1.25:1, an ratio of 2:1 at 60°C for 70 min. Lead oxalate precursor is calcined in air or N2, porous α-PbO is the only product in air and the calcination products are PbO and metallic Pb in N2 confirmed by morphology and structure characterizations, which is appropriate for lead-acid battery manufacturing. The recycling products were reused directly in lead-acid battery assembling as positive active materials and the assembled batteries show a good cyclic stability in 50 discharge/charge cycles.
研究领域:
化学工程
·
材料科学
·
环境科学
✓ 已验证
DOI
高影响因子
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
·
2020
·Vol. 324
·期 2
·715-729
·
IF 1.137
Wei Hongfu,Dong Faqin,Chen Mulan,Zhang Wei,He Miao,Liu Mingxue
As jarosite and oxalic acid are ubiquitous in nature, we investigated a remediation technology for uranium-contaminated site by photoinduced reduction coupled with adsorption of jarosite. Jarosite exhibited excellent adsorption properties for uranium, with a highest adsorption capacity 453.98 mg g −1 . With the presence of oxalic acid, U(VI) was removed at a reduction ratio of 30.71% under irradiation. It may be that oxalic acid promoted the reduction of Fe(III) to Fe(II) in jarosite, and then Fe(II) reduced U(VI) to U(IV). The results confirmed the applicability of the remediation by adsorption using jarosite and light-induced reduction.
研究领域:
环境科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
CANADIAN JOURNAL OF CHEMICAL ENGINEERING
·
2020
·Vol. 98
·期 11
·2321-2329
·
IF 1.687
Guojing Ji,Jie Ding,Qin Zhong
This study focused on the performances and kinetics of the transesterification reaction of diethyl oxalate with methanol to prepare ethyl methyl oxalate via microreactor technology. The conversion of 79.8% of diethyl oxalate (DEO) and the selectivity of 65.9% of ethyl methyl oxalate (EMO) was obtained under the following optimized conditions: the mole ratio of methanol to diethyl oxalate was 3.3:1, the temperature was 35°C, the K 2 CO 3 catalyst concentration was 15 mg/mL, and the residence time was 2.30 minutes. In the temperature range of 25°C to 38°C, the simplified dynamic model was found to obtain the reaction order (α = 2.30), frequency factor (k 0 = 2.377 × 10 5 ), and apparent activation energy (E = 31.86 kJ/mol). The macroscopic dynamic equation was derived from the experimental result of the transesterification reaction of two feedstocks, which can be obtained through a series of calculations.
研究领域:
化学工程
·
催化反应
·
有机合成
✓ 已验证
DOI
高影响因子
Molecular Catalysis
·
2024
·
IF 3.9
Zuwei Luo,Yun Shen,Di Fang,Chuanchao He,Yueqiang Cao,Wei Li,Yi-An Zhu,Jinghong Zhou,Xinggui Zhou
Support materials play vital roles in heterogenous catalysis via the so-called support effects, and understanding on the support effects is beneficial for the design of high-performance catalysts. Herein, we represent detailed investigations on the support effects of Ag catalysts for dimethyl glycolate (DMO) semi-hydrogenation to methyl glycolate (MG), where G6 silica with amorphous mesopores, SBA-15 with parallel mesopores and silica nanospheres (MSNS) with center-radial ones were employed as the supports. The hydrogenation activity decreases in the order of Ag/MSNS > Ag/SBA-15 > Ag/G6 while the MG selectivity in the order of Ag/G6 > Ag/MSNS > Ag/SBA-15 at the same reaction conditions, indicating remarkable support effects on both activity and selectivity. Structural characterizations, temperature programmed experiments and in-situ FTIR of DMO adsorption experiments demonstrate that the weak confinement effect of G6 leads to the larger sized Ag nanoparticles and weakened activation of DMO and H 2 , which contributes to low hydrogenation activity. In contrast, the ordered mesoporous SBA-15 and MSNS deliver well confinement effects for Ag nanoparticles and the activation of DMO and H 2 , but the distinct pore structures result in different activation and diffusion behaviors. Finally, DMO conversion and MG yield are well correlated with the competitive adsorption of H 2 versus DMO on Ag catalysts, indicating such competitivity as a descriptor for DMO semi-hydrogenation over Ag catalysts.
研究领域:
催化化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED CLAY SCIENCE
·
2024
·
IF 5.3
Fengyuan Wang,Min Yang,Yuchun Yang,Yanling Tian
With the escalating market demand for lithium, the development and efficient utilization of lithium resources have become crucial. This study introduced a method for leaching lithium from raw clay-type lithium ores using a composite sulfuric acid oxalic acid system. The experimental results revealed that the optimal conditions for the leaching process included roasting temperature of 600 °C, sulfuric acid concentration of 0.8 mol/L, liquid-solid ratio of 5 mL/g, leaching temperature of 90 °C, leaching duration of 90 min, and oxalic acid dosage of 2 g. Under these conditions, the leaching efficiency of lithium reached 93.45 %. The structural changes during the lithium leaching process and leaching mechanism were analyzed by XRD, SEM, TOF-SIMS. It was found that the minerals after mixed-acid leaching showed a loose morphology, a decrease in the average particle size, and a significant increase in the specific surface area as well as the pore volume, leading to improved lithium leaching efficiency. Furthermore, the mechanism underlying the mixed-acid leaching of lithium from clay-type lithium ores was explored. According to this mechanism, sulfuric acid first dissociated H + , which disrupted the mineral structure, allowing further destruction by oxalic acid. During this process, Li + was continuously replaced by H + and reacted with C 2 O 4 2− dissociated from oxalic acid to form water-soluble Li 2 C 2 O 4 .
研究领域:
化学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2020
·
IF 2.627
Dongmei Jia,Huamin Cai,Yongzheng Duan,Jiangbao Xia,Jia Guo
Chromium is a common harmful pollutant with high toxicity and low bearing capacity of soil and water. Excellent salinity resistance, a wide pH range, and high regeneration capacity were essential for qualified adsorbents used in removing hexavalent chromium (Cr(VI)) from polluted water. Herein, iron oxalate modified weak basic resin (IO@D301) for the removal of Cr(VI) was prepared by the impregnation method. The IO@D301 was characterized by scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), X-Ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). Owing to abundant amine, carboxyl groups and iron ions existing on the surface, IO@D301 possesses high adsorption and salinity resistance capacity for Cr(VI). The maximum adsorption capacity of IO301 towards Cr(VI) reached 201.30 mg·g −1 at 293 K and a pH of 5. The adsorption equilibrium was well fitted by the Freundlich model, and the adsorption process was described by the pseudo-first-order kinetics model as spontaneous and exothermic. The mechanism may be identified as electrostatic attraction, coordination, and reduction, which was confirmed by FT-IR and X-ray photoelectron spectroscopy.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
TEXTILE RESEARCH JOURNAL
·
2020
·
IF 1.926
Chen Li,Lijie Wang,Hong Xu,Jinxiang Dong
The builder is an important ingredient in detergents. Sodium oxalate is a good non-phosphate detergent builder, which has good calcium-removing properties and good wash performance in detergents. However, sodium oxalate-based detergent formulations face a tricky problem of “incrustation” on fabrics due to calcium oxalate deposition. If sodium oxalate is used in commercial detergents, then “incrustation” must be solved. In this study, we examined the effect of fabric type, anionic surfactant, polymer, and temperature on incrustation and soil redeposition (“secondary” washing effect) of a sodium oxalate-based detergent. The results showed that both the anionic surfactant and polymer influenced the deposition of calcium oxalate on fabrics. Overall, the amount of calcium oxalate deposition followed the order polyamide < polyester < cotton with the same formulation. Finally, good secondary washing effects (low ash deposition and high whiteness retention) formulations were selected by cumulative washing. The formulation of methyl ester sulfonate (MES)-sodium carboxymethyl cellulose (CMC), MES-polyvinyl pyrrolidone, and alcohol ether sulfate (AES)-CMC had better secondary effects, and the optimal combination of the 12 formulations was AES-CMC.
研究领域:
材料科学
·
表面化学
·
纺织工程
✓ 已验证
DOI
高影响因子
CRYSTAL GROWTH & DESIGN
·
2020
·
IF 4.089
Si Li,Weiwei Tang,Peng Shi,Mengya Li,Jie Sun,Junbo Gong
The therapeutics for nephrolithiasis have created an awareness of the potential effects of crystal modifiers on the biocrystallization process. The crystallization of calcium oxalate, a primary constituent of kidney stones, is confirmed to be inhibited by natural or synthetic modifiers, but the induction of calcium oxalate dihydrate and trihydrate, which is likewise effective for retardation of stone formation, is rarely focused on. Herein, we performed a systematic investigation of the effect of gallic acid, a natural polyphenol, on the nucleation of calcium oxalate from both experimental and simulation approaches. Our results reveal that the solution species of gallic acid alter the nucleation pathways of calcium oxalate and induce the formation of different types of amorphous structures corresponding to calcium oxalate hydrates. Different species of gallic acid regulate the formation of three types of amorphous calcium oxalates (ACOs) by incorporating the proper water contents and specific interactions. Fluorescence microscopy further demonstrates clearly the incorporation of gallic acid in the nucleated calcium oxalate precipitates. These findings unveil the nucleation mechanism of different calcium oxalate hydrates with the inclusion of modifiers and provide insights for an understanding of stone formation and its potential inhibitors.
研究领域:
材料科学
·
生物物理学
·
化学
·
医学
✓ 已验证
DOI
高影响因子
JOURNAL OF CLINICAL LABORATORY ANALYSIS
·
2021
·Vol. 35
·期 8
·e23870
·
IF 2.352
Ying Shen,Xia Luo,Huijun Li,Qing Guan,Liming Cheng
Background Urinary oxalate can provide important clues for the screening and monitoring of children with primary hyperoxaluria (PH), which is a potentially life-threatening condition. However, little effort has been devoted to improve the oxalate assay in recent years. We have proposed a reliable and cost-effective high-performance liquid chromatography (HPLC) method for urinary oxalate determination. Methods Urine specimens were centrifuged after one-step derivatization, and the supernatants were subjected to HPLC analysis. Results The method was validated with consistent linearity from 0.0625 to 2.0 mmol/L with coefficients of variation ≤7.73%, good recovery, low carryover, satisfactory sample stability, and analytical specificity. The lower limit of quantification and the limit of detection were 0.03130 and 0.0156 mmol/L, respectively. Imprecision values were ≤2.92% and ≤16.6% for externally and internally produced controls, respectively. The pediatric reference interval of spot urinary oxalate to creatinine ratios was established together with its application in screening of PH in patients with renal diseases, revealing its successful deployment in our laboratory. Conclusions This reliable HPLC method could serve as a significant tool to determine urinary oxalate levels for screening and monitoring of children with PH in routine clinical laboratories.
研究领域:
医学检验
·
临床化学
·
儿科医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2023
·Vol. 12
·期 9
·1761
·
IF 5.2
Chunyan Zhu,Yunchang Fan,Xiujun Bai
Rosmarinus officinalisleaves (ROLs) are widely used as a popular culinary spice for flavoring food, in which carnosic acid (CA) and rosmarinic acid (RA) are the main active components. The extraction of CA and RA is limited by lowextraction efficiency and extraction rate. In this work, a microwave-assisted extraction (MAE) method using biodegradable, low-toxic and nonflammable solvents polyethylene glycols (PEGs) as extraction solvents was developed for theextraction of CA and RA from ROLs. Experimental results suggest that PEG-400 was a better choice compared with PEG-200, and the optimal extraction conditions were as follows: 45% of PEG-400, 4.3% of phosphoric acid, 20 s of microwave irradiation time at 280 W of microwave irradiation power, and a 10 mg mL−1solid–liquid ratio, respectively. The tissue structures of ROLs could be effectively disrupted by PEG-based MAE, leading to high CA and RA extraction efficiencies. The PEG-400 extract exhibited stronger 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging ability compared with butylated hydroxytoluene (BHT). Finally, compared with heating reflux extraction, ultrasound-assisted extraction, maceration, and MAE using ionic liquid and ethanol as extraction solvents, the developed PEG-400 based MAE exhibited the highest extraction ability and fastest extraction rate for CA and RA. These findings suggest that MAE using PEGs as extraction solvents is a promising method for the separation of bioactive compounds from natural plants.Keywords:antioxidant activity;green solvents;microwave-assisted extraction (MAE);Rosmarinus officinalisleaves (ROLs);carnosic acid (CA);rosmarinic acid (RA)
研究领域:
植物化学
·
食品科学
·
天然产物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOCHEMICAL SYSTEMATICS AND ECOLOGY
·
2025
·
IF 2
Lue Hong,Zerong Wu,Jiahui Gong,Jiahui Ma,Shengzhi Liu
Alocasia cucullata (Lour.) Schott is a member of the Araceae family and is characterized by its unique leaf morphology and vibrant flowers; thus, it is a valuable addition to gardens and interior decor. However, A. cucullata may contain toxic compounds, including calcium oxalate crystals, lectins, and alkaloids, and their distribution within the plant tissues remains poorly understood. In this study, untargeted metabolomics was used to analyse the distribution of the bioactive compounds across the roots, stems, leaves, and guttation of A. cucullata . The toxic effects of these tissues on the MDA-MB-231 human breast cancer cell line were evaluated using the MTT assay. Microscopic techniques were utilized to examine the microstructure of the guttation and stems, and the focus was on the morphology of the calcium oxalate crystals. Liquid chromatography and ELISA kits were used to quantify the calcium oxalate and lectin contents in the various tissues. The results demonstrated that the active constituents, primarily fatty acids, phenylpropanoids, flavonoids, peptides, and alkaloids, were concentrated in the roots. Notably, guttation exhibited no toxicity and lacked both calcium oxalate crystals and significant lectin levels. This research enhances the understanding of the bioactive composition and safety profile of A. cucullata and provides a theoretical foundation for future scientific investigations and safe cultivation, with implications for both scientific and industrial applications.
研究领域:
植物学
·
毒理学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）