MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Zhao-Liang Wang,Yu-Han Shen,Xin Sun,Ze-Hao Li,Xin-Yu Wang,Zheng Zhao
Hydrogen peroxide (H 2 O 2 ) quantification in biomedicine is an important indicator for different biological tissues. In this study, we developed a visual detection for H 2 O 2 sensing based on gold nanozyme-silk fibroin (AuNCs-SF) hydrogel hybrid system with good compatibility between silk fibroin hydrogel and BSA-AuNCs. The AuNCs-SF hydrogel exhibited a fast response to H 2 O 2 directly by fluorescence change and showed high sensitivity (limit of detection of 0.072 mM) and well stability. Moreover, the hydrogel possessed high selectivity for H 2 O 2 in FBS solution and excellent biocompatibility to L929 cells (mouse fibroblasts). Therefore, the AuNCs-SF hydrogel hybrid system has a great potential in the fields of clinical diagnosis for visual detection of H 2 O 2 .
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BioNanoScience
·
2025
·Vol. 15
·期 2
·1-10
·
IF 3.2
Cai He-Qing,Han Lu,Xue Xin-Yu,Zhang Kou,Wang Tian-Hao,Pan Fu-Hong,Sun Zhi-Cheng,Liu Ru-Ping,Hu Kun,Wei Yen
Gold nanocages (AuNCs) exhibit tunable local surface plasmon resonance (LSPR) properties and exceptional drug loading and releasing capabilities, attributes stemming from their hollow interiors and ultra-thin porous walls. These features underscore their promising applications in the biomedical field. In this study, we fabricated AuNCs with LSPR peaks at 810 nm via chemical etching of Ag nanocube templates with sharp edges using a precisely controlled volume of chloroauric acid. To impart gene-loading functionality, we chemically anchored thiol-modified polyethyleneimine (PEI) onto the surfaces of AuNCs through robust Au–S bonds, generating a novel gene carrier, AuNCs-PEI. Subsequently, we evaluated its photothermal efficacy using a femtosecond laser and assessed cytotoxicity via MTT assay. The binding affinity of AuNCs-PEI for EGFP plasmids was characterized by gel retardation assays. Furthermore, the gene delivery efficiency of AuNCs-PEI was examined in 293 T cells by quantifying green fluorescence protein signals using inverted fluorescence microscopy. These findings demonstrate that engineered AuNCs-PEI not only facilitates gene loading for therapeutic applications but also enables photothermal therapy (PTT).
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2025
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IF 4.6
Hong Qin,Zhixian Xie,Lixue Ren,Ruihan Chen,Wanjun Long,Shuo Wang,Luyu Wei,Liling Wang,Wei Lan,Hengye Chen,Haiyan Fu
Carbaryl, a broad-spectrum carbamate insecticide, is widely used to enhance agricultural productivity; however, its hazardous residues pose significant food safety risks. In this study, dual-emission nanohybrids (CD@AuNCs) were synthesized by leveraging the active groups on CDs and BSA-AuNCs. The nanohybrids facilitated the ratiometric fluorescence detection of Carbaryl based on an aggregation-induced emission enhancement (AIE) mechanism. The sensor exhibited a linear range of 1–100 mg/kg and a detection limit of 0.95 mg/kg, which is below the maximum residue limit for Carbaryl (5 mg/kg, Chinese National Standard GB 2763–2021). The method was validated through recovery experiments in actual food samples, yielding results consistent with those obtained by LC-MS analysis. This sensitive, stable, and ratiometric fluorescence method thus offers a reliable analytical strategy for detecting Carbaryl in food matrices, demonstrating significant potential for applications in food safety monitoring and pesticide residue analysis.
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JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2017
·
IF 2.625
Shuang Zhao,Zhengping Li,Yunxiao Li,Jun Yu,Guohua Liu,Ruping Liu,Zhao Yue
Herein we present the development of a bovine serum albumin (BSA)-coated fluorescent gold nanocluster (BSA-AuNC)/graphene oxide (GO) hybrid nanocomposite-based photoelectrochemical (PEC) biosensor, which can be used for multichannel detection of both hydrogen peroxide (H 2 O 2 ) and dopamine (DA) within one chip at negative and positive potentials, respectively. BSA-AuNC-based biosensors generally exhibit poor PEC sensing performance owing to their poor separation of photoexcited electron-hole pairs and low electrode coverage. Consequently, improved AuNC/GO hybrid nanocomposites and a layer-by-layer (LBL) method are introduced in this study. The enhancement was investigated by exploring the optimum concentration of GO (0.12 mg mL −1 ) and LBL number (four layers) for the preparation of the BSA-AuNC materials, revealing that GO plays different enhancement roles in the detection of H 2 O 2 and DA. Compared to the results from other gold nanoclusters with different ligands, the photoelectrical enhancement of BSA-AuNCs can be achieved for both negative and positive potentials simultaneously with the same optimum GO concentration and LBL number. Thus, the detection of H 2 O 2 and DA was achieved by BSA-AuNC/GO multilayers with enhanced sensing properties and easier fabrication processes. The two enhancement methods acting together led to an improvement in the limits of detection (LODs) from 325 μM to 23 μM (H 2 O 2 ) and from 7.45 μM to 1.5 μM (DA). Thus, our strategy shows great potential for the improvement of PEC sensing structure, and provides a method for multichannel detection.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2026
·
IF 4.1
Shen Fei,Tang Yuzhe,Meng Xiangzhi,Hou Juan,Sun Jing
Iron regulates redox processes and maintains cellular functions. In this study, a CDs@AuNCs-based ratiometric fluorescence probe was established for selective visual recognition and quantitative analysis of Fe 2+ . The probe combines blue-emitting carbon dots (CDs) and red-emitting gold nanoclusters (AuNCs), generating dual fluorescence emissions with distinct peaks at 435 nm and 610 nm. The I 610 /I 435 ratio was optimized for Fe 2+ detection, achieving excellent sensitivity with a detection limit as low as 3.5 nM. The proposed probe demonstrated superior photostability, high environmental tolerance, and remarkable selectivity towards Fe 2+ over other metal ions and small molecules. Real sample analysis, including ferrous succinate tablets, environmental water, and juice, showed satisfactory recoveries and low RSDs, confirming the reliability and accuracy of the method. CDs@AuNCs ratiometric probe is a promising tool for practical applications in clinical diagnostics, pharmaceutical quality control, and environmental monitoring, offering advantages such as simplicity, rapid response, and cost-effectiveness for Fe 2+ ion detection in complex real samples. Graphical
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Biosensors-Basel
·
2021
·Vol. 11
·期 6
·174
·
IF 5.519
Fanfan Xiao,Yuting Yu,Yang Wu,Lili Tian,Guoyan Zhao,Hailong Pang,Jie Du
In this paper, we propose a simple colorimetric method for the sensitive and selective detection of alkaline phosphatase (ALP) activity based on the turn off/turn on oxidase mimic activity of[email protected][email protected]/graphene oxide hybrids ([email protected]/GO) were easily obtained using the self-assembly method with poly (diallyldimethylammonium chloride) (PDDA)-coated GO and showed high oxidase-like activity compared with[email protected]We found that the pyrophosphate ion (P2O74−, PPi) could effectively inhibit the oxidase mimic activity of[email protected]/GO, and the hydrolysis of PPi by ALP restored the inhibited activity of[email protected]/GO, enabling them to efficiently catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to generate the blue oxidized product oxTMB. The intensity of the color showed a linear dependency with the ALP activity. ALP was detected in the linear range of 0–40 mU/mL with a low detection limit (LOD) of 0.26 mU/mL (S/N = 3). The proposed method is fast, easy, and can be applied to monitor the ALP activity in serum samples accurately and effectively, which suggests its practicability and reliability in the detection of ALP activity in clinical practice.Keywords:[email protected]/GO;oxidase-like activity;PPi;ALP;colorimetric detection
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Analytical Methods
·
2023
·
IF 3.1
Huanzong Zhang,Binhuang Cai,Fan Cai,Mingzhe Lian,Yinghui Wang
Gastric cancer is a prevalent malignant tumor of the gastrointestinal tract accompanied by a high mortality rate; therefore, early gastric cancer screening is critical for improving patient survival. In this study, we present a facile fluorescence immunoassay for highly sensitive screening of pepsinogen I (PG I) based on a one-pot biomimetic mineralization process for the synthesis of gold nanocluster-anchored manganese dioxide (AuNCs/MnO2) nanosheets. MnO2 first quenches the fluorescence of AuNCs through the Förster resonance energy transfer effect, whereas the introduction of ascorbic acid (AA) leads to the decomposition of MnO2 and rapidly recovers the fluorescence of AuNCs. Based on the above principles and phenomena, we developed a sensitive fluorescence immunoassay for the in situ generation of AA to detect PG I. Specifically, in the presence of PG I, the sandwich-type immunoreactivity-enriched alkaline phosphatase-labeled secondary antibody catalyzes the production of AA from the substrate, which enhances the fluorescence intensity. Under optimized conditions, the fluorescence intensity increased linearly with the concentration of PG I (0.05 to 200 ng mL−1) with a limit of detection (LOD) of 0.013 ng mL−1 (S/N = 3). The designed sensing platform has good stability (more than one year) and excellent anti-interference capability and demonstrates satisfactory accuracy for detection in real samples compared to commercial ELISA kits.
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Micro & Nano Letters
·
2019
·Vol. 14
·期 9
·952-956
·
IF 0.975
Yining Zhu,Wang Li,Cheng Ju,Xiang Gong,Wenhao Song,Yu Zhao,Ruijun Li
A bovine serum albumin-stabilised gold nanoclusters/Cu 2+ (BSA-Au NCs/Cu 2+ ) system was constructed as a fluorescent sensor for the ‘off–on’ detection of glutathione (GSH). The fluorescence of BSA-Au NCs could be effectively quenched by Cu 2+ due to the strong affinity between BSA-Au NCs and Cu 2+ . Then, coordination of Cu 2+ to GSH enabled the recovery of the fluorescence of BSA-Au NCs. To assess the selectivity of the BSA-Au NCs/Cu 2+ for GSH, K + , Mg 2+ , Ca 2+ , glucose, cysteine, tryptophan, and phenylalanine were examined under identical conditions, and all of them did not induce noticeable responses to BSA-Au NCs/Cu 2+ except for Cys. There was a good linear dependence of fluorescence intensity on the concentration of GSH in the range of 0.4–20.0 μM with the detection limit of 0.36 μM. The proposed sensor showed excellent performance in the selective detection of GSH.
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ANALYST
·
2019
·Vol. 144
·期 8
·2523-2530
·
IF 4.019
Xi-Jin Wu,Fan Kong,Chun-Qin Zhao,Shou-Nian Ding
Ratiometric fluorescent nanosensors were developed to detect mercury ions (Hg2+) using enhanced dual emissions from glutathione stabilized gold nanoclusters/indium-based metal–organic frameworks modified with cysteine (AuNCs/MIL-68(In)-NH2/Cys). The nanosensors exhibited bright pink fluorescence with AuNCs evenly distributed on MIL-68(In)-NH2. Under 370 nm excitation, the obtained sensor presented double fluorescence emission around 438 nm and 668 nm, which was attributed to MIL-68(In)-NH2 and GSH-AuNCs, respectively. The fluorescence emission was remarkably enhanced after modification with Cys. In the presence of Hg2+, the red fluorescence peak at 668 nm was quenched, while the blue fluorescence peak at 438 nm was slightly altered. The prepared AuNCs/MIL-68(In)-NH2/Cys nanosensors exhibited two linear ranges for the detection of Hg2+, namely from 20 pM to 0.2 μM and 0.2 μM to 60 μM, with a detection limit of 6.7 pM. They also presented high selectivity towards other ions and good performance in real water samples. Moreover, a radial star-shaped microfluidic paper-based analytical device (μPAD), as a straightforward and convenient platform, was successfully fabricated for the visual detection of Hg2+ with a wide detection range from 5 nM to 50 μM.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Li Hou,Juanjuan Huang,Shendong Liu,Tianran Lin,Shulin Zhao
The three-dimensional liposome networks are fabricated and act as signal amplification modules for the fabrication of a magneto-controlled fluorescent immunosensor, in which antibody-conjugated magnetic bead (MB-Ab), streptavidin-biotin recognition and liposome amplification as the triple signal amplification pathway. The Aptamer-Liposome-AuNCs (Apt-Lip-AuNCs) is employed as a fluorescence signal probe, which can release abundant of AuNCs by Triton-X 100 to produce fluorescence emission signal upon being excited at 369 nm. In the presence of target CEA, the labeled anti-CEA on the MB and the immobilized aptamer on the Apt-Lip-AuNCs sandwich the target CEA. Upon addition of streptavidin and Biotin-Lip-AuNCs, three-dimensional liposome networks are formed by a streptavidin-biotin reaction due to the capture of streptavidin for Biotin-Lip-AuNCs and the residual biotin site of liposome on the Apt-Lip-AuNCs, thereby increasing the loading capacity of AuNCs. CEA can be linearly assayed in the range from 0.05 ng mL −1 to 40 ng mL −1 with a detection limit of 13.2 pg mL −1 (3 σ/K ). It provided a concise sensing platform for CEA determination and showed a great potential application for on-site testing in early clinical diagnosis.
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ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Jing Xiong,Kailai Xu,Xiandeng Hou,Peng Wu
The excellent fluorescence property of Au nanoclusters (AuNCs) has received great attention for various chemosensing and biorelated applications, but the sample matrix is still an important problem that causes undesirable fluorescence variation. On the one hand, hydride generation (HG) is an effective strategy to separate the target analyte from the complex sample matrices, but the implementation of HG with AuNC-based fluorescent assays was not realized. On the other hand, due to the ultrasmall size of AuNCs and good catalytic performance of Au, AuNCs are also featuring intriguing catalytic applications. Herein, we proposed a new type of AuNC-based fluorescence assay for Se(IV) detection, in which hydride generation of Se(IV) was coupled with the fluorescence/catalytic dual functions of AuNCs. In a batch hydride generation mode, Se(IV) was first converted to volatile H2Se. When it spread in the headspace to contact with AuNCs supported paper, AuNC-catalyzed oxidation of H2Se by O2 to yield elemental selenium occurred, which further deposited on the surface of AuNCs to induce fluorescence quenching. The catalytic effect of AuNCs was studied in depth via both experimental and theoretical (density functional theory) investigations. Three main steps for H2Se oxidation were identified, with energy barriers in the presence of AuNCs significantly lower than those without. Benefiting from the reduced matrix interference by hydride generation and the unique catalysis/fluorescence of AuNCs, the proposed assay featured high selectivity, good sensitivity, and simplicity, with successful applications for selenium detection in real samples.
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ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Xiaoli Wu,Peiling Wu,Mengying Gu,Jian Xue
Blood glucose measurement plays a very important role in clinical diagnosis and fluorescence analysis has attracted extensive attention. A novel ratiometric fluorescent system with aggregation induced emission (AIE) property for the detection of glucose was established in this work. In this system, bovine serum albumin-stabilized Au nanoclusters (BSA-AuNCs) served both as the fluorescent detection probe and the AIE inducer. An AIE molecule, named sodium 1,2-bis [4-(3-sulfonatopropoxyl) phenyl]-1,2-diphenylethene (BSPOTPE), served as fluorescent reference probe. In the presence of H 2 O 2 , the fluorescence intensity of BSPOTPE/BSA-Au NCs at 680 nm progressively decreased while that at 490 nm remained constant. Glucose can be catalyzed by glucose oxidase (GOx) and produces H 2 O 2 . Therefore, glucose detection can be conveniently achieved by the proposed strategy. The fluorescence intensity change ratio increased linearly with the glucose concentration in the range 1–8 mM. Moreover, the proposed method exhibits a consecutive fluorescence color change (“from red to cyan”) to glucose concentration in the range of 1–8 mM under a 365 nm UV lamp and exhibits bright “red” or bright “cyan” in lower glucose concentrations (lower than 3 mM) or high glucose concentrations (higher than 7 mM), respectively. The work offers an ideal rapid clinical diagnostic method for both normal and abnormal blood glucose screening.
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